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serratia fonticola as an infectious agent. 19921396739
serratia fonticola isolated from a leg abscess.serratia fonticola was isolated from a very large leg abscess in a patient following an accident. this is the first documented human infection due to s. fonticola.19911890188
characterization of enterobacteria by esterase specific-activity profiles.the spectrum of specific activities and the electrophoretic mobilities of esterases produced by 550 strains of enterobacteriaceae belonging to 36 species and subclassified into six groups (group 1, escherichia coli, shigella and escherichia hermanii; group 2, genus salmonella and genus citrobacter; group 3, genus klebsiella and genus enterobacter; group 4, genus serratia and serratia fonticola; group 5, genus proteus, genus providencia and genus morganella; and group 6, genus yersinia) were anal ...19902202780
isolation of serratia fonticola from birds.eight hundred and sixty-seven strains of gram-negative rods obtained from fecal specimens of 90 wild european birds were isolated on selective media. eighteen of these were identified as serratia fonticola both biochemically and by dna hybridization. the biochemical characteristics of the new strains are compared with published results.19863739460
[identification of a cephalosporinase susceptible to clavulanic acid in a clinical strain of serratia fonticola].we analyzed the beta-lactamase production of a serratia fonticola isolated for its resistance to cefuroxime (minimum inhibitory concentration > 256 mg/l) in december 1993 from a patient hospitalized in meaux. the wild strain was resistant to amoxycillin but sensitive to augmentin, that suggested the production of a beta-lactamase susceptible to clavulanic acid. for the wild strain, beta-lactamase production was inducible and only one enzyme with an isoelectric point of 8.12 was detected. beta-la ...19957567122
evaluation of rapid one system for identification of 379 strains in the family enterobacteriaceae and oxidase-negative, gram-negative nonfermenters.the ability of the rapid one system (innovative diagnostic systems, inc., norcross, ga.) to identify 364 strains in the family enterobacteriaceae and 15 oxidase-negative, gram-negative, nonfermentative rods was evaluated. kits were inoculated with no. 2 mcfarland standard suspensions, and reactions were interpreted after 4 h of incubation at 35 degrees c. overall, the method correctly identified (to the species level or to the genus level for salmonellas and non-shigella sonnei shigella species) ...19948027345
incidence of histamine-forming bacteria and histamine content in scombroid fish species from retail markets in the barcelona area.incidence and diversity of histidine decarboxylating bacteria were determined in samples of tunafish, bonito and mackerel purchased at different retail markets. histamine-forming bacteria occurred in a low proportion and always accounted for less than 0.1% of the total bacterial load in the fish samples studied. similarly, histamine content in fish samples also was low ( < 25 ppm) and all of them met current histamine standards established by the european union. histamine was found in 83.3% of t ...19968652349
minimum growth temperatures of hafnia alvei and other enterobacteriaceae isolated from refrigerated meat determined with a temperature gradient incubator.minimum growth temperatures of hafnia alvei (n = 156) and other enterobacteriaceae isolates (n = 162) from refrigerated meat samples (n = 88) and control strains of h. alvei (n = 81) from clinical and environmental samples were determined with a plate-type continuous temperature gradient incubator on nutrient agar. the dominant species, hafnia alvei and serratia liquefaciens had mean minimum growth temperatures of 2.6 (range, 0.2-3.7 degrees c) and 1.7 (range, 0.2-2.6 degrees c), respectively. v ...19979105940
novel class a beta-lactamase sed-1 from citrobacter sedlakii: genetic diversity of beta-lactamases within the citrobacter genus.citrobacter sedlakii 2596, a clinical strain resistant to aminopenicillins, carboxypenicillins, and early cephalosporins such as cephalothin, but remaining susceptible to acylureidopenicillins, carbapenems, and later cephalosporins such as cefotaxime, was isolated from the bile of a patient treated with beta-lactam and quinolone antibiotics. the isolate produced an inducible class a beta-lactamase of pi 8.6, named sed-1, which was purified. characterized by a molecular mass of 30 kda, sed-1 pref ...200111451687
biochemical-genetic characterization of the chromosomally encoded extended-spectrum class a beta-lactamase from rahnella aquatilis.from whole-cell dna of a clinical isolate of the enterobacterial species rahnella aquatilis, a beta-lactamase gene was cloned that encoded a chromosomally encoded ambler class a enzyme, rahn-1. rahn-1, with a pi of 7.2, shares 76, 73, and 71% amino acid identity with the extended-spectrum beta-lactamase of chromosomal origin from serratia fonticola and with the plasmid-mediated beta-lactamases ctx-m-2 and ctx-m-1, respectively. the hydrolysis spectrum of the clavulanic acid-inhibited rahn-1 was ...200111557504
evolution of the gyrb gene and the molecular phylogeny of enterobacteriaceae: a model molecule for molecular systematic studies.phylogenetic trees showing the evolutionary relatedness of enterobacteriaceae based upon gyrb and 16s rrna genes were compared. congruence among trees of these molecules indicates that the genomes of these species are not completely mosaic and that molecular systematic studies can be carried out. phylogenetic trees based on gyrb sequences appeared to be more reliable at determining relationships among serratia species than trees based on 16s rrna gene sequences. gyrb sequences from serratia spec ...200211931166
natural antimicrobial susceptibilities of strains of 'unusual' serratia species: s. ficaria, s. fonticola, s. odorifera, s. plymuthica and s. rubidaea.the natural susceptibility to 71 antibiotics of 104 serratia strains of serratia ficaria (n = 15), serratia fonticola (n = 18), serratia odorifera (n = 16), serratia plymuthica (n = 32) and serratia rubidaea (n = 23) was examined. mics were determined using a microdilution procedure in isosensitest broth for all the strains and in cation-adjusted mueller-hinton broth for some strains. with few exceptions, all species tested were uniformly naturally resistant to penicillin g, oxacillin, cefazolin ...200312654765
sfh-i, a subclass b2 metallo-beta-lactamase from a serratia fonticola environmental isolate.an environmental isolate of serratia fonticola resistant to carbapenems was shown to contain a genetic determinant encoding a metallo-beta-lactamase of the subclass b2. the sfh-i enzyme exhibits some divergence from the previously characterized enzymes of this subclass. this is the first example of a naturally occurring metallo-beta-lactamase in enterobacteriaceae.200312821491
molecular characterization of a carbapenem-hydrolyzing class a beta-lactamase, sfc-1, from serratia fonticola utad54.an environmental isolate of serratia fonticola resistant to carbapenems contains a gene encoding a class a beta-lactamase with carbapenemase activity. the enzyme was designated sfc-1. the bla(sfc-i) gene is contained in the chromosome of s. fonticola utad54 and is absent from other s. fonticola strains.200415155245
bacteriology of a bear bite wound to a human: case report.human contact with bears has become more frequent, as has the resultant bear maulings and bite injuries. we report the bacteriology of a patient bitten by a grizzly bear (ursus arctos) from the rocky mountains foothills area east of banff national park, alberta, canada. the patient received field care, including metronidazole and cefazolin. subsequent deep-wound cultures grew serratia fonticola, serratia marcescens, aeromonas hydrophila, bacillus cereus, and enterococcus durans but no anaerobes.200415243122
isolation of serratia fonticola from skin lesions in a nile crocodile (crocodylus niloticus) with an associated septicaemia.this paper describes the first isolation of serratia fonticola in a nile crocodile (crocodylus niloticus). this organism was initially isolated from skin lesions and blood and subsequently from a variety of organs during necropsy. s. fonticola was confirmed as the pathogen causing the infection.200817451977
competitive inhibition bacteria of bovine origin against salmonella serovars.studies were conducted to isolate bacteria inhibitory to salmonella enterica serovar typhimurium definitive type (dt) 104 in vitro from cattle not carrying salmonella and to determine the inhibitory activity of the isolated bacteria through competitive growth in cattle feces artificially contaminated with salmonella typhimurium dt104 and s. enterica serovar newport. fecal samples (108) were obtained from dairy and beef cows. s. enterica serovars were isolated from 9.25% of the samples and includ ...200717803135
molecular identification of coliform bacteria isolated from drinking water reservoirs with traditional methods and the colilert-18 system.the accuracy of a traditional method (lactose utilization with acid and gas production) for the detection of coliform bacteria and e. coli was tested in comparison with method iso 9308-1 (based on acid formation from lactose) and the colilert-18 system (detection of beta-galactosidase). a total of 345 isolates were identified after isolation from water samples using api 20e strips. the colilert-18 led to the highest number of positive findings (95% of the isolates were assigned to coliforms), wh ...200817870668
biochemical characterization of sfc-1, a class a carbapenem-hydrolyzing beta-lactamase.the carbapenem-hydrolyzing beta-lactamase sfc-1 from serratia fonticola utad54 was overexpressed in escherichia coli, purified, and characterized. the enzyme exhibited an apparent molecular mass of 30.5 kda, determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. sfc-1 hydrolyzes penicillins, cephalosporins, aztreonam, and carbapenems and is inhibited by clavulanic acid, sulbactam, and tazobactam.200717875998
serratia glossinae sp. nov., isolated from the midgut of the tsetse fly glossina palpalis gambiensis.we report the isolation of a novel bacterium, strain c1(t), from the midgut of the tsetse fly glossina palpalis gambiensis, one of the vector insects responsible for transmission of the trypanosomes that cause sleeping sickness in sub-saharan african countries. strain c1(t) is a motile, facultatively anaerobic, rod-like bacterium (0.8-1.0 microm in diameter; 2-6 microm long) that grows as single cells or in chains. optimum growth occurred at 25-35 degrees c, at ph 6.7-8.4 and in medium containin ...201019667382
molecular and biochemical characterization of the natural chromosome-encoded class a beta-lactamase from pseudomonas luteola.pseudomonas luteola (formerly classified as cdc group ve-1 and named chryseomonas luteola) is an unusual pathogen implicated in rare but serious infections in humans. a novel beta-lactamase gene, bla(lut-1), was cloned from the whole-cell dna of the p. luteola clinical isolate lam, which had a weak narrow-spectrum beta-lactam-resistant phenotype, and expressed in escherichia coli. this gene encoded lut-1, a 296-amino-acid ambler class a beta-lactamase with a pi of 6 and a theoretical molecular m ...201019884377
novel ambler class a carbapenem-hydrolyzing beta-lactamase from a pseudomonas fluorescens isolate from the seine river, paris, france.a pseudomonas fluorescens isolate (pf-1) resistant to carbapenems was recovered during an environmental survey performed with water from the seine river (paris). it expressed a novel ambler class a carbapenemase, bic-1, sharing 68 and 59% amino acid identities with beta-lactamases sfc-1 from serratia fonticola and the plasmid-encoded kpc-2, respectively. beta-lactamase bic-1 hydrolyzed penicillins, carbapenems, and cephalosporins except ceftazidime and monobactams. the bla(bic-1) gene was chromo ...201019901091
effects of bovine milk lactoperoxidase system on some bacteria.bovine lactoperoxidase (lpo) was purified from skimmed milk using amberlite cg-50-h+ resin, cm sephadex c-50 ion-exchange chromatography, and sephadex g-100 gel filtration chromatography. lactoperoxidase was purified 20.45-fold with a yield of 28.8%. purity of enzyme checked by sodium dodecyl sulphate-polyacrylamide gel electrophoresis method and a single band was observed. km was 0.25 mm at 20 degrees c, vmax value was 7.95 micromol/ml min at 20 degrees c (ph 6.0). antibacterial study was done ...201020198919
bio-reduction of fe(iii) ores using three pure strains of aeromonas hydrophila, serratia fonticola and clostridium celerecrescens and a natural consortium.the present work describes a research approach to the anaerobic bioleaching of fe(iii) ores. three strains (serratia fonticola, aeromonas hydrophila and clostridium celerecrescens) isolated from an acidic abandoned mine were selected to test their ability to reduce dissimilatory fe(iii). total iron bio-reduction was achieved after 48h using either the consortium or the aeromonas cultures. in the latter case, there was no evidence of precipitates and fe(ii) remained in solution at neutral ph thro ...201020627716
crystal structure of serratia fonticola sfh-i: activation of the nucleophile in mono-zinc metallo-ß-lactamases.metallo-ß-lactamases (mbls) or class b ß-lactamases are zinc-dependent enzymes capable of inactivating almost all classes of ß-lactam antibiotics. to date, no mbl inhibitors are available for clinical use. of the three mbl subclasses, b2 enzymes, unlike those from subclasses b1 and b3, are fully active with one zinc ion bound and possess a narrow spectrum of activity, hydrolyzing carbapenem substrates almost exclusively. these remain the least studied mbls. sfh-i, originally identified from the ...201121762699
predominant enterobacteria on modified-atmosphere packaged meat and poultry.enterobacteria on modified-atmosphere (ma) packaged meat (n = 54) and poultry (n = 32) products were enumerated, and 899 isolates were picked and ribotyped. for identification, 16s rrna genes of representative strains were sequenced and analyzed. altogether 54 (60%) of the samples contained enterobacteria >10(4) cfu/g. in 34% of the poultry samples, enterobacteria counts were >10(6) cfu/g suggesting that enterobacteria may contribute to spoilage of ma packaged poultry. the enterobacteria identif ...201223541191
quorum sensing activity of serratia fonticola strain rb-25 isolated from an ex-landfill site.quorum sensing is a unique bacterial communication system which permits bacteria to synchronize their behaviour in accordance with the population density. the operation of this communication network involves the use of diffusible autoinducer molecules, termed n-acylhomoserine lactones (ahls). serratia spp. are well known for their use of quorum sensing to regulate the expression of various genes. in this study, we aimed to characterized the ahl production of a bacterium designated as strain rb-2 ...201424625739
adhesion to brown trout skin mucus, antagonism against cyst adhesion and pathogenicity to rainbow trout of some inhibitory bacteria against saprolegnia parasitica .biological control of saprolegniosis with bacteria might be an alternative to the use of chemical compounds. among criteria for the selection of such bacteria are their absence of pathogenicity to fish and their ability to prevent adhesion of the pathogen to the skin mucus. the pathogenicity to rainbow trout of 21 bacterial isolates with in vitro inhibitory activity against saprolegnia parasitica was studied. fifteen of the isolates, identified as aeromonas sobria, pantoea agglomerans, pseudomon ...201323670078
on the active site of mononuclear b1 metallo β-lactamases: a computational study.metallo-β-lactamases (mβls) are zn(ii)-based bacterial enzymes that hydrolyze β-lactam antibiotics, hampering their beneficial effects. in the most relevant subclass (b1), x-ray crystallography studies on the enzyme from bacillus cereus point to either two zinc ions in two metal sites (the so-called '3h' and 'dch' sites) or a single zn(ii) ion in the 3h site, where the ion is coordinated by asp120, cys221 and his263 residues. however, spectroscopic studies on the b1 enzyme from b. cereus in the ...201222532071
in vitro and in vivo antimicrobial activity of propolis on the microbiota from gastrointestinal tract of chickens.the aim of this study was to examine the effect of propolis extracts on the microbial colonization of chicken gastrointestinal tract in vivo. the propolis was administered to both feed mixtures in various amounts except of the control group. the addition of 150 mg propolis to 1 kg of feed was included in the first experimental group, the addition of 450 mg.kg(-1) in the second experimental group, the addition of 600 mg.kg(-1) the third experimental group and 800 mg kg(-1) in the fourth one. the ...201222702827
serum bactericidal assay: new role in salmonella detection.while inspecting animal feed for salmonella contamination, we routinely observed bacterial colonies on selective agars that were similar in appearance to those formed by salmonella. these were identified as citrobacter freundii, proteus mirabilis, and serratia fonticola using biochemical and serological techniques. because the presence of these bacterial species confounds identification of salmonella, we refer to them as "interference bacteria." polyvalent antisera against these interference bac ...201726822285
prevalence and characterization of esbl- and ampc-producing enterobacteriaceae on retail vegetables.in total 1216 vegetables obtained from dutch stores during 2012 and 2013 were analysed to determine the prevalence of 3rd-generation cephalosporin (3gc) resistant bacteria on soil-grown fresh produce possibly consumed raw. vegetables grown conventionally and organically, from dutch as well as foreign origin were compared. included were the following vegetable types; blanched celery (n=192), bunched carrots (n=190), butterhead lettuce (n=137), chicory (n=96), endive (n=188), iceberg lettuce (n=19 ...201525828704
extended spectrum ß-lactamase- and constitutively ampc-producing enterobacteriaceae on fresh produce and in the agricultural environment.the attribution of fresh produce to the overall community-associated exposure of humans to esbl- or ampc-producing bacteria is currently unknown. to address this issue, the prevalence of esbl- and ampc-producing enterobacteriaceae on fresh produce produced in the netherlands was determined. seven vegetable types that are consumed raw were selected: blanched celery, bunched carrots, chicory, endive, iceberg lettuce, mushrooms, and radish. the vegetables were mostly obtained from supermarkets. to ...201424211774
susceptibility patterns of uropathogens identified in hospitalised children with community-acquired urinary tract infections in thrace, greece.urinary tract infections (utis) are common infections associated with considerable morbidity and mortality, particularly in paediatric patients. the alarmingly increasing antimicrobial resistance of contemporary uropathogens in children necessitates the re-evaluation of antibiotic treatment. we evaluated uropathogens isolated from children hospitalised due to a community-acquired uti over a 5.5-year period in a university hospital of northern greece and their antibiotic susceptibility patterns. ...201527873675
a geospatial analysis of flies and the spread of antimicrobial resistant bacteria.livestock is often colonized with esbl-producing enterobacteriaceae (esbl-e) and staphylococcus aureus. there is a risk that flies spread antimicrobial resistant bacteria from livestock to humans. here, we screened flies from urban and rural areas near the city of münster, germany, for the presence of esbl-e and s. aureus and compared molecular characteristics of these isolates with those isolated from humans in the same region. in total, 1346 single flies were grinded and cultured overnight in ...201627345493
listeria monocytogenes-carrying consortia in food industry. composition, subtyping and numerical characterisation of mono-species biofilm dynamics on stainless steel.in order to find out how real listeria monocytogenes-carrying biofilms are in industrial settings, a total of 270 environmental samples belonging to work surfaces from fish (n = 123), meat (n = 75) and dairy industries (n = 72) were analysed in order to detect l. monocytogenes. 12 samples were positive for l. monocytogenes and a total of 18 different species were identified as accompanying microbiota in fish and meat industry. no l. monocytogenes was found in samples from dairy industry. molecul ...201526001376
characterization of an extended-spectrum class a β-lactamase from a novel enterobacterial species taxonomically related to rahnella spp./ewingella spp.to characterize the naturally occurring β-lactamase gene identified from a clinical isolate belonging to a novel enterobacterial species that is closely related to rahnella spp. and ewingella spp.201323580557
chania multitudinisentens gen. nov., sp. nov., an n-acyl-homoserine-lactone-producing bacterium in the family enterobacteriaceae isolated from landfill site soil.phylogenetic and taxonomic characterization was performed for bacterium rb-25t, which was isolated from a soil sample collected in a former municipal landfill site in puchong, malaysia. growth occurred at 20-37 °c at ph 5-8 but not in the presence of 9 % (w/v) nacl or higher. the principal fatty acids were c16:0, c18:1ω7c and summed feature 3 (c16:1ω7c and/or iso-c15:0 2-oh). ubiquinone-8 was the only isoprenoid quinone detected. polar lipid analysis revealed the presence of phospholipid, phosph ...201626978486
serratia glossinae geiger et al. 2010 is a later synonym of serratia fonticola gavini et al. 1979.serratia glossinae dsm 22080(t) was compared with serratia fonticola atcc 29844(t) to clarify the taxonomic relationship of both species. 16s rrna gene sequence comparisons demonstrated that these species share 99.6% sequence similarity. investigation of substrate utilization profiles displayed no striking differences from the type strains of both species. dna-dna hybridizations between both strains showed 100% (99.9%) similarity. therefore, the reclassification of s. glossinae as a later synony ...201525667392
density- and trait-mediated effects of a parasite and a predator in a tri-trophic food web.despite growing interest in ecological consequences of parasitism in food webs, relatively little is known about effects of parasites on long-term population dynamics of non-host species or about whether such effects are density or trait mediated. we studied a tri-trophic food chain comprised of (i) a bacterial basal resource (serratia fonticola), (ii) an intermediate consumer (paramecium caudatum), (iii) a top predator (didinium nasutum) and (iv) a parasite of the intermediate consumer (holospo ...201525382389
draft genome sequence of plant-growth-promoting rhizobacterium serratia fonticola strain au-ap2c, isolated from the pea rhizosphere.plant health can be augmented by plant-growth-promoting rhizobacteria (pgpr) that confer biofertilizer, phytostimulation, and biocontrol activities. herein, we provide the high-quality draft genome sequence of serratia fonticola strain au-ap2c, a gram-negative motile pgpr of the pea plant, conferring phosphate solubilization, ammonia production, and antifungal activity against fusarium sp. the 4.9-mb genome contains genes related to plant growth promotion and synthesis of siderophores.201324309742
serratia oryzae sp. nov., isolated from rice stems.a novel endophytic bacterium, strain j11-6t, was isolated from rice stems. its taxonomic position was investigated using a polyphasic approach. the novel strain was gram-staining-negative, facultatively anaerobic, motile and rod-shaped. although the results of phylogenetic analysis based on 16s rrna gene sequences indicated that j11-6t represented a member of the genus rahnella, multilocus sequence analysis (mlsa) on the basis of concatenated partial atpd, gyrb, rpob and infb gene sequences show ...201728853686
overlapping podospora anserina transcriptional responses to bacterial and fungal non self indicate a multilayered innate immune response.recognition and response to non self is essential to development and survival of all organisms. it can occur between individuals of the same species or between different organisms. fungi are established models for conspecific non self recognition in the form of vegetative incompatibility (vi), a genetically controlled process initiating a programmed cell death (pcd) leading to the rejection of a fusion cell between genetically different isolates of the same species. in podospora anserina vi is c ...201627148175
serratia fonticola, pathogen or bystander? a case series and review of the literature.serratia fonticola is an unusual human pathogen, previously described primarily as causing skin and soft tissue infections following trauma. there is little information in the literature about its treatment or susceptibilities. we describe the case of a 67-year-old male with paraplegia who developed urosepsis due to s. fonticola. blood and urine cultures obtained prior to the initiation of antimicrobials both grew s. fonticola. the patient completed a 15-day course of antimicrobials and had an u ...201627347484
microbial studies of a selenium-contaminated mine site and potential for on-site remediation.surface water selenium (se) concentrations are above regulatory standards at several active and inactive phosphate mine sites in the us western phosphate resource area. the focus of the present study was to examine the impacts of the microbial communities on the oxidation state of se in overburden waste from the smoky canyon phosphate mine in idaho, usa. microbial populations were found that reduce soluble selenate (seo (4) (2-) ) to insoluble elemental se. microcosm experiments were conducted f ...200616804682
characterization of serratia isolates from soil, ecological implications and transfer of serratia proteamaculans subsp. quinovora grimont et al. 1983 to serratia quinivorans corrig., sp. nov.eleven strains of serratia were isolated from different soils and the guts of invertebrates and characterized by their sensitivity to eight indigenous bacteriophages. they were also classified according to bacteriocin production and sensitivity, biolog plate and api 20e strip profiles and 16s rrna sequence information. one strain was thus identified as serratia plymuthica, another as serratia fonticola. the remaining strains were shown to be closely related to serratia proteamaculans subsp. quin ...200212508898
identification of a chromosome-borne expanded-spectrum class a beta-lactamase from erwinia persicina.from whole-cell dna of an enterobacterial erwinia persicina reference strain that displayed a penicillinase-related antibiotic-resistant phenotype, a beta-lactamase gene was cloned and expressed in escherichia coli. it encoded a clavulanic-acid-inhibited ambler class a beta-lactamase, erp-1, with a pi value of 8.1 and a relative molecular mass of ca. 28 kda. erp-1 shared 45 to 50% amino acid identity with the most closely related enzymes, the chromosomally encoded enzymes from citrobacter koseri ...200212384342
gene transfer and plasmid instability within pilot-scale sewage filter beds and the invertebrates that live in them.the environmental plasmid pqkh6 was transferred conjugatively between strains of pseudomonas putida at mean frequencies of up to 8.4x10(-4) within pilot-scale sewage filter beds. this frequency was 10-fold higher than that reported previously for this environment and was probably due to seasonal temperature changes. many (45%) of the plasmids isolated subsequently from the filter beds had restriction fragment length polymorphism (rflp) profiles that differed from that expected for pqkh6. rflp an ...200111295459
characterization of sfo-1, a plasmid-mediated inducible class a beta-lactamase from enterobacter cloacae.enterobacter cloacae 8009 produced an inducible class a beta-lactamase which hydrolyzed cefotaxime efficiently. it also hydrolyzed other beta-lactams except cephamycins and carbapenems. the activity was inhibited by clavulanic acid and imipenem. the bla gene was transferable to escherichia coli by electroporation of plasmid dna. the molecular mass of the beta-lactamase was 29 kda and its pi was 7.3. all of these phenotypic characteristics of the enzyme except for inducible production resemble th ...19999925524
characterization and amino acid sequence analysis of a new oxyimino cephalosporin-hydrolyzing class a beta-lactamase from serratia fonticola cuv.serratia fonticola cuv produces two isoenzymes (forms i and ii) with beta-lactamase activity which were purified by a five-step procedure. the isoenzymes had identical kinetic parameters and isoelectric point (pi = 8.12). they were characterized by a specific activity towards benzylpenicillin of 1650 u/mg. the beta-lactamase hydrolyzed benzylpenicillin, amoxycillin, ureidopenicillins, first- and second-generation cephalosporins. carboxypenicillins and isoxazolylpenicillins were hydrolyzed to a l ...19979300809
sheen formation and growth response of groundwater bacteria to reduced oxygen concentrations during incubation of m-endo medium.in vitro pure-culture studies were conducted to assess growth and sheen formation of groundwater bacteria on m-endo medium incubated under reduced oxygen concentrations (0, 4, 8, 12, and 16%). coliform and noncoliform bacteria were isolated from 17 untreated, rural groundwater supplies on m-endo medium. all 16 coliform isolates tested were capable of sheen formation at oxygen concentrations of 4% or greater, yet some of these same isolates (enterobacter aerogenes, enterobacter cloacae, and hafni ...19921393829
childhood delayed septic arthritis of the knee caused by serratia fonticola.we report a first documented case of serratia fonticola infection in a child with septic arthritis, after falling off his bicycle and an infection with hawthorn thorns. the aetiologic agent was found to be a s. fonticola strain which was first isolated from two synovial fluid samples, only after culture enrichment, with failure of dna amplification. accurate diagnostic was done, despite s. fonticola is often considered as an opportunistic pathogenic bacterium. alone ciprofloxacin treatment was n ...200919401267
rapid detection of serratia fonticola by taqman quantitative real-time pcr using primers targeting the gyrb gene.a gyrb gene is present in the majority of bacterial species, and encodes the atpase domain of dna gyraseb-subunit protein, which is essential for transcription and replication of bacteria. the gyrb gene exhibits higher nucleotide sequence variability than the 16s rdna gene and thus could be more reliable in differentiating serratia fonticola. a species-specific primer pair and probe were designed for quantitative real-time pcr detection of s. fonticola using gyrb as the target gene. nine members ...201728821942
prevalence and characterization of extended-spectrum β-lactamase (esbl) and ampc β-lactamase producing enterobacteriaceae in fresh pork meat at processing level in germany.esbl or ampc β-lactamase producing enterobacteriaceae is an increasing concern in human medicine. a distribution via the food chain is discussed, but less is known about these bacteria on fresh pork meat. the aim of this study was to investigate the prevalence of esbl/ampc enterobacteriaceae bacteria in fresh pork meat at processing level in germany. the analysis comprised microbiological hygiene parameters and further pheno- and genotypical characterization of esbl/ampc isolates. the examinatio ...201728646667
misidentification of providencia stuartii as serratia fonticola by vitek 2. 201425265761
Biochemical characterization of Sfh-I, a subclass B2 metallo-beta-lactamase from Serratia fonticola UTAD54.The subclass B2 metallo-ß-lactamase (MBL) Sfh-I from Serratia fonticola UTAD54 was cloned and overexpressed in Escherichia coli. The recombinant protein binds one equivalent of zinc, as shown by mass spectrometry, and preferentially hydrolyzes carbapenem substrates. However, compared to other B2 MBLs, Sfh-I also shows limited hydrolytic activity against some additional substrates and is not inhibited by a second equivalent of zinc. These data confirm Sfh-I to be a subclass B2 metallo-ß-lactamase ...201121876065
assessment of bacterial biodetoxification of herbicide atrazine using aliivibrio fischeri cytotoxicity assay with prolonged contact time.in our study, we determined and compared the atrazine-biodetoxification ability of 41 bacterial strains and 21 consortia created of those with over 50% degradation rate in pure cultures. biodegradation capacity was measured with gc-ms. detoxification was assessed based on the cytotoxic effect of end-products to aliivibrio fischeri in chronic bioluminescence inhibition assay with 25 h contact time. chronic a. fischeri assay adapted to a microplate, which is suitable for examine numerous residues ...201728466204
the basis for carbapenem hydrolysis by class a β-lactamases: a combined investigation using crystallography and simulations.carbapenems are the most potent β-lactam antibiotics and key drugs for treating infections by gram-negative bacteria. in such organisms, β-lactam resistance arises principally from β-lactamase production. although carbapenems escape the activity of most β-lactamases, due in the class a enzymes to slow deacylation of the covalent acylenzyme intermediate, carbapenem-hydrolyzing class a β-lactamases are now disseminating in clinically relevant bacteria. the reasons why carbapenems are substrates fo ...201223030300
draft genome sequence of a plant growth-promoting rhizobacterium, serratia fonticola strain au-p3(3).plant growth-promoting rhizobacteria (pgpr), found in the rhizospheric region of plants, not only suppress plant disease, but also directly improve plant health by improving the availability of nutrients and by providing phytostimulants. herein, we report the high-quality genome sequence of serratia fonticola strain au-p3(3), a pgpr of the pea plant, which confers phosphate solubilization, indole-3-acetic acid production, ammonia production, hydrogen cyanide (hcn) production, and siderophore pro ...201324233592
draft genome sequence of serratia fonticola lmg 7882t isolated from freshwater.serratia fonticola is a gram-negative bacterium with a wide distribution in aquatic environments. on some occasions, it has also been regarded as a significant human pathogen. in this work, we report the first draft genome sequence of an s. fonticola strain (lmg 7882(t)), which was isolated from freshwater.201324265499
draft genome sequence of serratia fonticola utad54, a carbapenem-resistant strain isolated from drinking water.serratia fonticola utad54 is an environmental isolate that is resistant to carbapenems due to the presence of a class a carbapenemase and a metallo-β-lactamase that are unique to this strain. its draft genome sequence was obtained to clarify the molecular basis of its carbapenem resistance and identify the genomic context of its carbapenem resistance determinants.201324285645
complete genome sequence of serratia fonticola dsm 4576 t, a potential plant growth promoting bacterium.here, we present the first complete genome sequence of serratia fonticola dsm 4576(t), a potential plant growth promoting (pgp) bacterium which confers solubilization of inorganic phosphate, indole-3-acetic acid production, hydrogen cyanideproduction, siderophore production and assimilation of ammonia through the glutamate synthase (gs/gogat) pathway. this genome sequence is valuable for functional genomics and ecological studies which are related to pgp and biocontrol activities.201526376471
serratia aquatilis sp. nov., isolated from drinking water systems.a cream-white-pigmented, oxidase-negative bacterium (strain 2015-2462-01t), isolated from a drinking water system, was investigated in detail to determine its taxonomic position. cells of the isolate were rod-shaped and stained gram-negative. a comparison of the 16s rrna gene sequence of strain 2015-2462-01t with sequences of the type strains of closely related species of the genus serratia revealed highest similarity to serratia fonticola (98.4 %), serratia proteamaculans (97.8 %), serratia liq ...201626537514
genomic and phenotypic analyses of serratia fonticola strain gs2: a rhizobacterium isolated from sesame rhizosphere that promotes plant growth and produces n-acyl homoserine lactone.the genus serratia incorporates many agriculturally important species. serratia fonticola strain gs2, isolated from a sesame rhizosphere, can produce n-acyl homoserine lactone quorum sensing signal molecules and indole-3-acetic acid (iaa). here we report the complete genome sequence and phenotypic characteristics based on genomic information of this bacterium. the complete genome sequence of s. fonticola strain gs2 consists of a chromosome of 6.1 mbp and two plasmids of 132 kbp and 94 kbp. the g ...201727923736
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