| differentiation of allium carlaviruses isolated from different parts of the world based on the viral coat protein sequence. | common primers which amplify the 3' terminal genomic rnas of allium carlaviruses were designed based on the nucleotide sequence of shallot latent virus (slv), garlic latent virus (glv) and garlic common latent virus (gclv). a total of fifteen cdnas encoding the coat protein (cp) of the carlaviruses, including the biologically identified isolates slv, glv and gclv as well as viruses from infected allium plants cultivated in different parts of the world, were amplified by rt-pcr with the common pr ... | 1998 | 9687867 |
| the global trade in fresh produce and the vagility of plant viruses: a case study in garlic. | as cuisine becomes globalized, large volumes of fresh produce are traded internationally. the potential exists for pathogens infecting fresh produce to hitchhike to new locations and perhaps to establish there. it is difficult to identify them using traditional methods if pathogens are novel, scarce, and/or unexpected. in an attempt to overcome this limitation, we used high-throughput sequencing technology as a means of detecting all rna viruses infecting garlic (allium sativum l.) bulbs importe ... | 2014 | 25133543 |
| simultaneous detection of four garlic viruses by multiplex reverse transcription pcr and their distribution in indian garlic accessions. | indian garlic is infected with onion yellow dwarf virus (oydv), shallot latent virus (slv), garlic common latent virus (garclv) and allexiviruses. identity and distribution of garlic viruses in various garlic accessions from different geographical regions of india were investigated. oydv and allexiviruses were observed in all the garlic accessions, while slv and garclv were observed only in a few accessions. a multiplex reverse transcription (rt)-pcr method was developed for the simultaneous det ... | 2014 | 24598229 |
| viruses occurring in onion crop in amasya province, the major onion producing region in turkey. | amasya is the greatest onion producing area in turkey. onion fields from amasya region were surveyed for virus diseases in 2009-2011 and tested for the presence of the most important onion viruses such as onion yellow dwarf virus (oydv), iris yellow spot virus (iysv), leek yellow stripe virus (lysv), shallot latent virus (slv) and garlic common latent virus (gclv). the presence of virus diseases and their identification was ascertained through symptom observation in the fields, sap transmission ... | 2013 | 24426263 |
| molecular characterization of coat protein gene of garlic common latent virus isolates from india: an evidence for distinct phylogeny and recombination. | the coat protein (cp) gene of five indian garlic common latent virus (garclv) isolates was sequenced and it was 960 bp long in all the five isolates, encoding a protein of 319 amino acids. comparative nucleotide sequence analysis revealed diversity of 4.3% among the indian isolates and of 11.9% among all isolates worldwide. amino acid sequence comparison showed a significant variability in the n-terminal of cp of garclv. various protein analysis tools identified thirteen conserved domains and mo ... | 2013 | 23553322 |