| bud endophytes of scots pine produce adenine derivatives and other compounds that affect morphology and mitigate browning of callus cultures. | endophytes are found in meristematic bud tissues of scots pine (pinus sylvestris l.) especially prior to growth, which would suggest their involvement in growth of the bud. to test this hypothesis, production of phytohormones by two bacterial (methylobacterium extorquens, pseudomonas synxantha) and one fungal endophyte (rhodotorula minuta) was studied by mass spectrometry. the most common gibberellins, auxins, or cytokinins were not detected in the fractions studied. instead, m. extorquens and r ... | 2004 | 15153198 |
| molecular bacterial diversity and bioburden of commercial airliner cabin air. | culture-independent, biomarker-targeted bacterial enumeration and identification strategies were employed to estimate total bacterial burden and diversity within the cabin air of commercial airliners. samples from each of 4 flights on 2 commercial carriers were collected via air-impingement. the total viable microbial population ranged from below detection limits to 4.1 x 10(6) cells/m(3) of air, as assessed by the atp assay. a gradual accumulation of microbes was observed from the time of passe ... | 2007 | 18026220 |
| regulation of pyrimidine nucleotide biosynthesis in pseudomonas synxantha. | regulation of pyrimidine nucleotide biosynthesis in pseudomonas synxantha atcc 9890 was investigated and the pyrimidine biosynthetic pathway enzyme activities were affected by pyrimidine supplementation in cells grown on glucose or succinate as a carbon source. in pyrimidine-grown atcc 9890 cells, the activities of four de novo enzymes could be depressed which indicated possible repression of enzyme synthesis. to learn whether the pathway was repressible, pyrimidine limitation experiments were c ... | 2007 | 17578676 |
| reduction of cr(vi) and bioaccumulation of chromium by gram positive and gram negative microorganisms not previously exposed to cr-stress. | resistance to cr(vi) is usually associated with its cellular exclusion, precluding enrichment techniques for the isolation of organisms accumulating cr(vi) via bioreduction to insoluble cr(iii). a technique was developed to screen for potential cr(vi) reduction in approx. 2000 isolates from a coastal environment, based on the non-specific reduction of selenite and tellurite to se0 and te0, and reduction of tetrazolium blue to insoluble blue formazan. the most promising strains were further scree ... | 2002 | 12164635 |
| physical mapping, bac-end sequence analysis, and marker tagging of the soilborne nematicidal bacterium, pseudomonas synxantha bg33r. | a bacterial artificial chromosome (bac) library was constructed for the genome of the rhizosphere-inhabiting fluorescent pseudomonad pseudomonas synxantha bg33r. three thousand bac clones with an average insert size of 140 kbp and representing a 70-fold genomic coverage were generated and arrayed onto nylon membranes. ecori fingerprint analysis of 986 bac clones generated 23 contigs and 75 singletons. hybridization analysis allowed us to order the 23 contigs and condense them into a single conti ... | 2002 | 11881828 |
| dna-based and culture-based characterization of a hydrocarbon-degrading consortium enriched from arctic soil. | a hydrocarbon-degrading consortium was enriched from fuel-contaminated soil from the northeastern tip of ellesmere island (82 degrees 30'n, 62 degrees 19'w). the enrichment culture was grown on jet a-1 fuel at 7 degrees c. bacterial 16s rna gene (rdna) fragments were amplified by polymerase chain reaction (pcr) from members of the above consortium and cloned into a plasmid vector. partial sequences (approximately 500 bp) were determined for 29 randomly selected rdna clones. the majority of seque ... | 2001 | 11822837 |
| detection of intracellular bacteria in the buds of scotch pine (pinus sylvestris l.) by in situ hybridization. | bacterial isolates were obtained from pine (pinus sylvestris l.) tissue cultures and identified as methylobacterium extorquens and pseudomonas synxantha. the existence of bacteria in pine buds was investigated by 16s rrna in situ hybridization. bacteria inhabited the buds of every tree examined, primarily colonizing the cells of scale primordia and resin ducts. | 2000 | 10877808 |
| population structure and diversity of phenazine-1-carboxylic acid producing fluorescent pseudomonas spp. from dryland cereal fields of central washington state (usa). | certain strains of the rhizosphere bacterium pseudomonas fluorescens contain the phenazine biosynthesis operon (phzabcdefg) and produce redox-active phenazine antibiotics that suppress a wide variety of soilborne plant pathogens. in 2007 and 2008, we isolated 412 phenazine-producing (phz(+)) fluorescent pseudomonas strains from roots of dryland wheat and barley grown in the low-precipitation region (<350 mm annual precipitation) of central washington state. based on results of box-pcr genomic fi ... | 2012 | 22383119 |
| biodegradation of waste greases and biochemical properties of a novel lipase from pseudomonas synxantha ps1. | a lipase-producing bacterial strain was isolated from oil-well-produced water in shengli oilfield (shandong province, china) and was identified as pseudomonas synxantha by 16s rdna sequence analysis (named pseudomonas synxantha ps1). strain ps1 showed a maximum lipase activity of 10.8 u/ml after culturing for 48 h at 30 °c, with lactose (4 g/l) as carbon source, tryptone (8 g/l) as nitrogen source, olive oil (0.5%, v/v) as inductor, and the initial ph 8.0. meanwhile, the lipase gene from p. synx ... | 2016 | 27321682 |
| diversity and biomineralization potential of the epilithic bacterial communities inhabiting the oldest public stone monument of cluj-napoca (transylvania, romania). | in this study, we investigated the biomineralization potential and diversity of the epilithic bacterial communities dwelling on the limestone statue of saint donatus, the oldest public monument of cluj-napoca city (transylvania region, nw romania). their spatial distribution together with phylogenetic and metabolic diversity, as well as their capacity to precipitate calcium carbonate was evaluated by combining molecular and phenotypic fingerprinting methods with x-ray diffraction, fourier transf ... | 2017 | 28326074 |
| bacterial biota of nigeen lake waters (kashmir valley). | one of the greatest apprehensions of water consumers all over the world with respect to the quality of drinking water is its contamination with pathogenic microorganisms. this research work determined the potential bacterial contaminants of the waters of nigeen lake, a subsidiary of dal lake and is regarded as a separate lake in kashmir. the study was carried out from may 2014 to november 2014 excluding august and september at four different sites. during the study the bacterial flora showed var ... | 2016 | 27165539 |
| metabolic pathway for a new strain pseudomonas synxantha lsh-7': from chemotaxis to uptake of n-hexadecane. | bacteria can use n-hexadecane as a carbon source, but it remains incompletely understood whether n-hexadecane is transformed into metabolic intermediates prior to cellular uptake or not. we newly isolated a strain identified as pseudomonas synxantha lsh-7' and conducted chemotaxis experiment of this bacterial strain towards n-hexadecane, hexadecanol and hexadecanoic acid with qualitative assays respectively. furthermore, we described the identification of extracellular alkane hydroxylase and alc ... | 2017 | 28051099 |
| the use of customised probiotics in the cultivation of western king prawns (penaeus latisulcatus kishinouye, 1896). | this study presents a comprehensive review of probiotics usage in aquaculture with a specific emphasis on our research series on the effectiveness of the customised probiotics, pseudomonas synxantha and pseudomonas aeruginosa on the cultivation of western king prawns, penaeus latisulcatus. these customised probiotics resulted from tests using five inhibition test methods between the bacteria isolated from two commercial probiotic products and vibrio spp. isolated from western king prawns and oth ... | 2009 | 19463955 |
| isolation and identification of phosphate solubilizing bacteria able to enhance the growth and aloin-a biosynthesis of aloe barbadensis miller. | the effect of four phosphate solubilizing bacteria (psb) was studied on growth and aloin-a content of aloe barbadensis in soil containing tricalcium phosphate (tcp). psb were identified based on 16s rrna gene sequencing as pseudomonas synxantha, burkholderia gladioli, enterobacter hormaechei and serratia marcescens. these psb solubilized 25-340 μg ml(-1) of tcp into the liquid phase. the treatment of plants with individual psb or mixture of these increased soil available p, p uptake in plants an ... | 2012 | 22417676 |
| autotransporter domain-dependent enzymatic analysis of a novel extremely thermostable carboxylesterase with high biodegradability towards pyrethroid pesticides. | the estps1 gene, which encodes a novel carboxylesterase of pseudomonas synxantha ps1 isolated from oil well-produced water, was cloned and sequenced. estps1 has an open reading frame of 1923 bp and encodes the 640-amino acid carboxylesterase (estps1), which contains an autotransporter (at) domain (357-640 amino acids). homology analysis revealed that estps1 shared the highest identity (88%) with esta from pseudomonas fluorescens a506 (ncbi database) and belonged to the carboxylesterase family (e ... | 2017 | 28615636 |
| bioactive compound from pseudomonas synxantha inhibits the growth of mycobacteria. | tuberculosis is a dreaded disease and the current situation demands new anti-tubercular agent(s) for the management of public health. towards this direction, we obtained a contaminant organism on a mycobacterium smegmatis lawn having growth inhibitory activity against the later. in the current study, efforts were targeted to identify this organism and characterize the bioactive compound from this isolate that inhibited the growth of mycobacteria. the result revealed that the organism is a strain ... | 2014 | 24439826 |