| synthesis of carbon-14 and tritium labeled sagamicin. | sagamicin, an aminoglycoside antibiotic, was labeled with tritium by means of a platinum catalyzed hydrogen exchange reaction and by carbon-14 in two biosynthetic procedures. 14c-methyl-l-methionine was used as the radioactive precursor in these biological processes involving micromonospora sagamiensis. the distribution of radioactivity in 14c-sagamicin and gentamicin c1 was studied by mild acid hydrolysis and hofmann degradation. the results showed that both c- and n-methyl groups were derived ... | 1977 | 591466 |
| the gentamicin antibiotics. 6. gentamicin c2b, an aminoglycoside antibiotic produced by micromonospora purpurea mutant ji-33. | a mutant strain of micromonospora purpurea, designated var. ji-33, produced an antibiotic complex consisting primarily of gentamicin c1a. a further product of this fermentation was identical to a very minor component isolated from the fermentation of the parent organism and named gentamicin c2b. physical measurements indicated its structure to be 6'-n-methylgentamicin c1a and this was confirmed by synthesis from gentamicin c1a. the in vitro antibacterial activity of gentamicin c2b was very simil ... | 1975 | 1092638 |
| multiple-dose pharmacokinetics in serum and sputum of sagamicin administered intramuscularly to patients. | sagamicin is an aminoglycosidic antibiotic produced by micromonospora sagamiensis, chemically related with gentamicin. single- and multiple-dose pharmacokinetics have been investigated in patients affected by bronchopulmonary infections treated with 60 mg sagamicin i.m. every 12 h. the two concentration-time curves for sputum and serum are parallel; while there is no evidence of accumulation in the serum at the 7th day of treatment, sagamicin concentrations in the sputum are significantly higher ... | 1987 | 3568801 |
| [safety evaluation of micronomicin iv. acute toxicity in rats, rabbits and dogs after drip intravenous infusion]. | micronomicin (mcr) is a new aminoglycoside antibiotic produced by micromonospora sagamiensis var. nonreducans which was isolated from soil collected at sagamihara city by nara et al. this antibiotic shows a close similarity to gentamicin c components in physical and chemical properties. the antibacterial activity of mcr is broad-spectrum and almost equal to that of gentamicin c complex. mcr exhibits particularly high activity against pseudomonas, proteus, klebsiella pneumoniae, serratia, etc, as ... | 1983 | 6674532 |
| [safety evaluation of micronomicin v. subacute toxicity in rats after intravenous injection]. | micronomicin (mcr) is a new aminoglycoside antibiotic produced by micromonospora sagamiensis var. nonreducans which was isolated from soil collected at sagamihara city by nara et al. this antibiotic shows a close similarity to gentamicin c components in physical and chemical properties. the antibacterial activity of mcr is broad-spectrum and almost equal to that of gentamicin c complex. mcr exhibits particularly high activity against pseudomonas, proteus, klebsiella pneumoniae, serratia, etc. as ... | 1983 | 6674533 |
| [safety evaluation of micronomicin vi. subacute toxicity in rabbits after drip intravenous infusion]. | micronomicin (mcr) is a new aminoglycoside antibiotic produced by micromonospora sagamiensis var. nonreducans which was isolated from soil collected at sagamihara city by nara et al. this antibiotic shows a close similarity to gentamicin c components in physical and chemical properties. the antibacterial activity of mcr is broad-spectrum and almost equal to that of gentamicin c complex. mcr exhibits particularly high activity against pseudomonas, proteus, klebsiella pneumoniae, serratia, etc. as ... | 1983 | 6674534 |
| [safety evaluation of micronomicin vii. fertility study by intravenous injection in rats]. | micronomicin (mcr) is a new aminoglycoside antibiotic produced by micromonospora sagamiensis var. nonreducans which was isolated from soil collected at sagamihara city by nara et al. this antibiotic shows a close similarity to gentamicin c components in physical and chemical properties. the antibacterial activity of mcr is broad-spectrum and almost equal to that of gentamicin c complex. mcr exhibits particularly high activity against pseudomonas, proteus, klebsiella pneumoniae, serratia, etc. as ... | 1983 | 6674535 |
| [safety evaluation of micronomicin viii. teratogenicity studies in rabbits after intravenous injection]. | micronomicin (mcr) is a new aminoglycoside antibiotic produced by micromonospora sagamiensis var. nonreducans which was isolated from soil collected at sagamihara city by nara et al. this antibiotic shows a close similarity to gentamicin c components in physical and chemical properties. the antibacterial activity of mcr is broad-spectrum and almost equal to that of gentamicin c complex. mcr exhibits particularly high activity against pseudomonas, proteus, klebsiella pneumoniae, serratia, etc. as ... | 1983 | 6674536 |
| [safety evaluation of micronomicin ix. perinatal and postnatal study by intravenous injection in rats]. | micronomicin (mcr) is a new aminoglycoside antibiotic produced by micromonospora sagamiensis var. nonreducans which was isolated from soil collected at sagamihara city by nara et al. this antibiotic shows a close similarity to gentamicin c components in physical and chemical properties. the antibacterial activity of mcr is broad-spectrum and almost equal to that of gentamicin c complex. mcr exhibits particularly high activity against pseudomonas, proteus, klebsiella pneumoniae, serratia, etc. as ... | 1983 | 6674537 |
| 2-hydroxysagamicin: a new antibiotic produced by mutational biosynthesis of micromonospora sagamiensis. | | 1982 | 6802790 |
| sagamicin and the related aminoglycosides: fermentation and biosynthesis. iii. isolation and characterization of micromonospora sagamiensis mutants blocked in gentamicin c1 pathway. | production of sagamicin and gentamicin c1 in micromonospora sagamiensis was regulated by cobalt ion. in a parental strain, ky11510, cobalt ion stimulated gentamicin c1 production and suppressed sagamicin production. by ultraviolet light or n-methyl-n'-nitro-n-nitrosoguanidine treatment, six mutants blocked in gentamicin c1 biosynthesis were obtained from ky11510. these mutants were classified into two types. the first type, four mutants, produced no gentamicin c1 even when cobalt ion was added t ... | 1983 | 6833127 |
| [safety evaluation of micronomicin. i. subacute and chronic toxicity in dogs]. | micronomicin (mcr) is a new aminoglycoside antibiotic produced by micromonospora sagamiensis var. nonreducans which was isolated from soil collected at sagamihara by nara et al. the purified antibiotic showed a close similarity to gentamicin c complex in physical and chemical properties. the antibacterial activity of mcr is broad-spectrum and almost equal to that of gentamicin c complex. mcr exhibits particularly high activity against pseudomonas, proteus, klebsiella pneumoniae, serratia, etc. a ... | 1983 | 6842822 |
| [safety evaluation of micronomicin. ii. irritation by repeated intravenous injection in dogs]. | micronomicin (mcr) is a new aminoglycoside antibiotic produced by micromonospora sagamiensis var. nonreducans which was isolated from soil collected at sagamihara by nara et al. the purified antibiotic showed a close similarity to gentamicin c complex in physical and chemical properties. the antibacterial activity of mcr is broad-spectrum and almost equal to that of gentamicin c complex. mcr exhibits particularly high activity against pseudomonas, proteus, klebsiella pneumoniae, serratia, etc. a ... | 1983 | 6842823 |
| [safety evaluation of micronomicin. iii. teratogenicity studies in rats]. | micronomicin (mcr) is a new aminoglycoside antibiotic produced by micromonospora sagamiensis var. nonreducans which was isolated from soil collected at sagamihara by nara et al. the purified antibiotic showed a close similarity to gentamicin c complex in physical and chemical properties. the antibacterial activity of mcr is broad-spectrum and almost equal to that of gentamicin c complex. mcr exhibits particularly high activity against pseudomonas, proteus, klebsiella pneumoniae, serratia, etc. a ... | 1983 | 6842824 |
| [review [new antibiotics series iii]: micronomicin (author's transl)]. | micronomicin is a new aminoglycosidic antibiotic discovered and developed by kyowa hakko kogyo co., ltd. it is produced by micromonospora sagamiensis var. nonreducans. investigation of micronomicin performed in 134 research facilities in japan led to the following results. 1) micronomicin showed a broad antibacterial spectrum against gram positive and gram negative bacteria. 2) in susceptibility tests of clinical isolates, micronomicin was almost similarly active to gm. 3) bactericidal activity ... | 1982 | 7047790 |
| sagamicin and the related aminoglycosides: fermentation and biosynthesis. i. biosynthetic studies with the blocked mutants of micromonospora sagamiensis. | the mutants blocked in the gentamicin c1 production were derived from a sagamicin producing strain of micromonospora sagamiensis. the intermediates produced by these mutants were isolated and properly identified. comparing the biotransformation activities in the resting cells of the mutants with those of a dos idiotroph, ky 11525, the blocked steps in sagamicin and gentamicin biosynthesis were proposed in each mutant. mutant ky 11564 was found to produce gentamicin c2a (c-6'-epimer of gentamicin ... | 1982 | 7082452 |
| production of antibiotic su-2 complex by a 2-deoxystreptamine idiotroph of micromonospora sagamiensis. | a 2-deoxystreptamine idiotrophic mutant of micromonospora sagamiensis, ky 11509, was found to produce unknown antibacterial substances, which were named su-2 complex. each component, su-1, su-2 and su-3 were isolated from a culture broth of ky 11509. chromatographic data suggested that these components were new antibiotics. the antibiotics exhibited potent and broad spectrum of antibacterial activity. the amount of su-1, su-2 and su-3 production reached their maximum level (197, 82 and 58 microg ... | 1982 | 7096193 |
| cell wall composition of micromonospora olivoasterospora, micromonospora sagamiensis, and related organisms. | cell walls of 19 micromonospora species were analyzed for their components. all the cell walls had xylose and arabinose, but the presence of glucose, galactose, mannose, or rhamnose depended on the strain. amino acids present in the walls consisted of glycine, glutamic acid, diaminopimelic acid, and alanine, in a molar ratio of approximately 1:1:1:0.6--0.8. 3-hydroxydiaminopimelic acid, together with meso-diaminopimelic acid, was found in many species and was isolated from micromonospora olivoas ... | 1981 | 7217010 |
| accumulation of 2-deoxy-scyllo-inosamine by a 2-deoxystreptamine-requiring idiotroph of micromonospora sagamiensis. | | 1980 | 7451373 |
| eight new species of the genus micromonospora, micromonospora citrea sp. nov., micromonospora echinaurantiaca sp. nov., micromonospora echinofusca sp. nov. micromonospora fulviviridis sp. nov., micromonospora inyonensis sp. nov., micromonospora peucetia sp. nov., micromonospora sagamiensis sp. nov., and micromonospora viridifaciens sp. nov. | a previous phylogenetic study on type strains of the genus micromonospora and micromonospora species bearing non-validly published names has pointed towards the species status of several of latter strains. subsequent studies on morphological, cultural, chemotaxonomic, metabolic, and genomic properties, and on whole cell mass spectrometric analyses by matrix adsorbed laser desorption/ionization time-of-flight (maldi-tof) confirmed the species status, leading to the proposal of eight new micromono ... | 2005 | 15997706 |
| micromonospora fluostatini sp. nov., isolated from marine sediment. | the novel actinomycete strain pwb-003t, which produced fluostatins b and c antibiotics, was isolated from nearshore sediment collected from panwa cape, phuket province, thailand. data from the present polyphasic study indicated that strain pwb-003t represented a member of the genus micromonospora. it produced single spores on substrate mycelia and contained meso-diaminopimelic acid in the cell-wall peptidoglycan. whole-cell hydrolysate contained ribose, xylose, arabinose, mannose and glucose. th ... | 2015 | 26358439 |
| femtosecond laser-based mutagenesis strategy for micronomicin production enhancement of micromonospora sagamiensis atcc 21826. | in this work, a mutant mx3004 with improved micronomicin (mcr) production was derived from micromonospora sagamiensis atcc21826, which was treated with femtosecond laser under the optimized irradiation conditions of 75 mw and 180 s, with a maximum of positive mutation rate of 17.8 % and the mortality rate of 69.2 %. a novel high-throughput method was established using microplate reader by quantifying the concentration of mcr for efficient screening of positive mutant from large numbers of mutant ... | 2013 | 23355136 |