| the amino acid sequence determination of a granulin and polyhedrin from two baculoviruses infecting agrotis segetum. | the amino acid sequence of agrotis segetum granulosis virus (asgv) granulin and a. segetum nuclear polyhedrosis virus (asnpv) polyhedrin was determined by sequencing tryptic and chymotryptic peptides from reduced and carboxymethylated proteins and tryptic fragments of oxidized and maleylated granulin. the comparison of the established peptide structures with the primary structures of other occlusion body proteins from related baculoviruses was also used for the polypeptide chains' reconstruction ... | 1992 | 1604817 |
| a simplified method for the characterization of nuclear polyhedrosis virus genomes. | a simplified restriction endonuclease analysis procedure is described which allows the characterization of baculovirus dna obtained directly from a single larvae without purification of virus. this rapid method was used to demonstrate the genomic stability of nuclear polyhedrosis viruses (npvs) from agrotis segetum, euxoa messoria and mamestra brassicae after several passages in euxoa scandens. | 1989 | 2550318 |
| adaptation of antennal neurons in moths is associated with cessation of pheromone-mediated upwind flight. | a wind-borne plume of sex pheromone from a female moth or a synthetic source has a fine, filamentous structure that creates steep and rapid fluctuations in concentration for a male moth flying up the plume's axis. the firing rates from single antennal neurons on agrotis segetum antennae decreased to nearly zero within seconds after the antennae were placed in a pheromone plume 70 cm downwind of a high-concentration source known from previous studies to cause in-flight arrestment of upwind progre ... | 1988 | 3200859 |
| electron microscopy of nuclear polyhedrosis virus from agrotis segetum schiff. and a. exclamationis l. (lepidoptera, noctuidae). | | 1971 | 4102572 |
| [a new heterophil agglutinin "anti-aas" in caterpillars and pupae of agrotis segetum (schiff) (author's transl)]. | | 1972 | 4283241 |
| [range of the hosts of the causative agent of pébrine (nosema bombycis) in the mulberry silkworm]. | biological interrelations between the agent of microsporidiosis of the silkworm and local species of insects were studied. 7 species of lepidoptera (agrotis segetum, chloridea obsoleta, laphygma exigua, plusia gamma, pieris brassicae, p. rapae, lymantria dispar) were infected with the suspension of nosema bombycis spores. results have shown that 6 species of insects are susceptible to nosema bombycis excluding only the gipsy moth. so, n. bombycis can be considered to be a parasite with a wide ra ... | 1980 | 6769083 |
| gut bacteria may be involved in interactions between plants, herbivores and their predators: microbial biosynthesis of n-acylglutamine surfactants as elicitors of plant volatiles. | n-acylamino acids are dominant and widespread constituents of insect oral secretions (regurgitants), serving the insect as biosurfactants in the digestive process. during feeding the conjugates may be introduced into damaged leaves and contribute there to the elicitation of plant defenses such as the induction of volatile biosynthesis. from gut segments of spodoptera exigua, mamestra brassicae and agrotis segetum 23 bacterial strains were isolated, ten of which were able to synthesise typical le ... | 2000 | 11030433 |
| immunolocalization of odorant-binding proteins in noctuid moths (insecta, lepidoptera). | odorant-binding proteins were studied in the noctuid moths agrotis segetum, autographa gamma, helicoverpa armigera, heliothis virescens and spodoptera littoralis using antisera raised against the pheromone-binding protein (pbp) and general odorant-binding protein 2 (gobp2) of antheraea polyphemus (saturniidae). proteins immunoreacting with these antisera were only found on the antennae and pbp and gobp2 could be identified on western blots of males and females of all five species. pbps were pred ... | 2001 | 11555483 |
| comparative study on the susceptibility of cutworms (lepidoptera: noctuidae) to agrotis segetum nucleopolyhedrovirus and agrotis ipsilon nucleopolyhedrovirus. | the common cutworm (agrotis segetum) and the black cutworm (agrotis ipsilon) are serious soil pests of many vegetable and field crops all over the world. we have demonstrated the cross-infectivity of two baculoviruses, a. segetum nucleopolyhedrovirus (agsenpv) and a. ipsilon nucleopolyhedrovirus (agipnpv) for these two insect pests. the susceptibility of a. segetum to agipnpv was confirmed by dna restriction endonuclease analyses of dna isolated from virus harvested from infected a. segetum larv ... | 2003 | 14615215 |
| molecular characterization of agrotis segetum nucleopolyhedrovirus from poland. | the turnip moth, agrotis segetum (lepidoptera, noctuidae), is an important pest insect in europe, asia, and africa. we have genetically characterized and classified a nucleopolyhedrovirus isolated from a. segetum larvae in poland (agsenpv-p). the restriction pattern of agsenpv-p was distinct from an isolate from england/france (agsenpv-uk and agsenpv-f). sequence analysis of three conserved baculovirus genes, polyhedrin, lef-8 and pif-2, revealed that agsenpv-p differs substantially from the alr ... | 2005 | 16055149 |
| genome sequence of an enhancin gene-rich nucleopolyhedrovirus (npv) from agrotis segetum: collinearity with spodoptera exigua multiple npv. | the genome sequence of a polish isolate of agrotis segetum nucleopolyhedrovirus (agsenpv-a) was determined and analysed. the circular genome is composed of 147,544 bp and has a g+c content of 45.7 mol%. it contains 153 putative, non-overlapping open reading frames (orfs) encoding predicted proteins of more than 50 aa, together making up 89.8 % of the genome. the remaining 10.2 % of the dna constitutes non-coding regions and homologous-repeat regions. one hundred and forty-three agsenpv-a orfs ar ... | 2006 | 16476975 |
| analysis of the genome of spodoptera frugiperda nucleopolyhedrovirus (sfmnpv-19) and of the high genomic heterogeneity in group ii nucleopolyhedroviruses. | the genome of the most virulent among 22 brazilian geographical isolates of spodoptera frugiperda nucleopolyhedrovirus, isolate 19 (sfmnpv-19), was completely sequenced and shown to comprise 132,565 bp and 141 open reading frames (orfs). a total of 11 orfs with no homology to genes in the genbank database were found. of those, four had typical baculovirus promoter motifs and polyadenylation sites. computer-simulated restriction enzyme cleavage patterns of sfmnpv-19 were compared with published p ... | 2008 | 18420798 |
| a functional f analogue of autographa californica nucleopolyhedrovirus gp64 from the agrotis segetum granulovirus. | the envelope fusion protein f of plutella xylostella granulovirus is a computational analogue of the gp64 envelope fusion protein of autographa californica nucleopolyhedrovirus (acmnpv). granulovirus (gv) f proteins were thought to be unable to functionally replace gp64 in the acmnpv pseudotyping system. in the present study the f protein of agrotis segetum gv (agsegv) was identified experimentally as the first functional gp64 analogue from gvs. agsef can rescue virion propagation and infectivit ... | 2008 | 18562524 |
| biological control of soil pests by mixed application of entomopathogenic and fungivorous nematodes. | in greenhouse experiments, massive application of the fungivorous nematode, aphelenchus avenae, in summer at 26-33 c (1 x l0 nematodes/500 cm(3) autoclaved soil) or in autumn at 18-23 c (5 x 10 nematodes/500 cm(3) autoclaved soil) suppressed pre-emergence damping-off of cucumber seedlings due to rhizoctonia solani ag-4 by 67% or 87%, respectively. application of 2 x l0 a. avenae to sterilized soil infested with r. solani caused leafminer-like symptom on the cotyledons, which did not occur in mix ... | 1991 | 19283109 |
| host-range expansion of spodoptera exigua multiple nucleopolyhedrovirus to agrotis segetum larvae when the midgut is bypassed. | given the high similarity in genome content and organization between spodoptera exigua multiple nucleopolyhedrovirus (semnpv) and agrotis segetum nucleopolyhedrovirus (agsenpv), as well as the high percentages of similarity found between their 30 core genes, the specificity of these npvs was analysed for the respective insect hosts, s. exigua and a. segetum. the ld(50) for agsenpv in second-instar a. segetum larvae was 83 occlusion bodies per larva and the lt(50) was 8.1 days. agsenpv was orally ... | 2010 | 19923260 |
| effects of bt maize on agrotis segetum (lepidoptera: noctuidae): a pest of maize seedlings. | the lepidopteran stemborers busseola fusca (fuller) (lepidoptera: noctuidae), sesamia calamistis (hampson) (lepidoptera: noctuidae), and chilo partellus (swinhoe) (lepidoptera: crambidae) are effectively controlled by bt maize that expresses the cry1ab insecticidal protein. another noctuid species, the cutworm agrotis segetum (denis and schiffermüller) (lepidoptera: noctuidae), which is the most common and injurious pest of maize seedlings in south africa, is exposed to bt toxin for a part of it ... | 2010 | 20388305 |
| analysis of the agrotis segetum pheromone gland transcriptome in the light of sex pheromone biosynthesis. | moths rely heavily on pheromone communication for mate finding. the pheromone components of most moths are modified from the products of normal fatty acid metabolism by a set of tissue-specific enzymes. the turnip moth, agrotis segetum uses a series of homologous fatty-alcohol acetate esters ((z)-5-decenyl, (z)-7-dodecenyl, and (z)-9 tetradecenyl acetate) as its sex pheromone components. the ratio of the components differs between populations, making this species an interesting subject for studi ... | 2015 | 26385554 |
| complete genome sequence of agrotis segetum granulovirus shanghai strain. | the complete nucleotide sequence of agrotis segetum granulovirus shanghai strain (agsegv-l1) was determined and compared with that of agsegv xinjiang strain (agsegv-xj). the circular genome of agsegv-l1 is 131,442 bp and has a g+c content of 37.27 %. it includes 149 orfs, 24 of which are unique to agsegv (agsegv-l1 and agsegv-xj [genbank accession no. nc_005839]). the average level of amino acid sequence identity between agsegv-l1 and other granulovirus (gv) homologues (except agsegv-xj) ranged ... | 2014 | 24519461 |
| the genome sequence of agrotis segetum nucleopolyhedrovirus b (agsenpv-b) reveals a new baculovirus species within the agrotis baculovirus complex. | the genome of agrotis segetum nucleopolyhedrovirus b (agsenpv-b) was completely sequenced and compared with whole genome sequences of the agrotis segetum nucleopolyhedrovirus a (agsenpv-a) and agrotis ipsilon nucleopolyhedrovirus (agipnpv). the agsenpv-b genome is 148,981 bp in length and encodes 150 putative open reading frames. agsenpv-b contains two copies of the gene viral enhancing factor (vef), making the agrotis nucleopolyhedroviruses and a. segetum granulovirus (agsegv) very rich in vef ... | 2015 | 25471493 |
| detection and quantitation of agrotis baculoviruses in mixed infections. | at least four distinct baculoviruses, namely the agrotis segetum nucleopolyhedrovirus a (agsenpv-a), the a. segetum nucleopolyhedrovirus b (agsenpv-b), the agrotis ipsilon nucleopolyhedrovirus (agipnpv) and the a. segetum granulovirus (agsegv) have been isolated from larval stages (cutworms) of the species a. segetum and a. ipsilon (lepidoptera: noctuidae), which are serious soil pests in agriculture. cutworms can become infected by at least one of these four baculoviruses and also co-infections ... | 2014 | 24291149 |
| genomic sequence analysis of the illinois strain of the agrotis ipsilon multiple nucleopolyhedrovirus. | the agrotis ipsilon multiple nucleopolyhedrovirus (agipmnpv) is a group ii nucleopolyhedrovirus (npv) from the black cutworm, a. ipsilon, with potential as a biopesticide to control infestations of cutworm larvae. the genome of the illinois strain of agipmnpv was completely sequenced. the agipmnpv genome was 155,122 nt in size and contained 163 open reading frames (orfs), including 61 orfs found among all lepidopteran baculoviruses sequenced to date. phylogenetic inference placed agipmnpv in a c ... | 2009 | 19015970 |
| vegetative insecticidal protein of bacillus thuringiensis blb459 and its efficiency against lepidoptera. | bacillus thuringiensis strain blb459 supernatant showed a promising activity against lepidopteran pests with extremely damages in the larvae midgut. investigations of the genes that encode secreted toxin demonstrated that this strain harbored a vip3-type gene named vip3(459). based on its original nucleotide and amino acid sequences, this gene was cloned into pet-14b vector and overexpressed in escherichia coli. the expressed protein was purified and tested against different insects and interest ... | 2017 | 28223048 |
| susceptibility of agrotis segetum (noctuidae) to bacillus thuringiensis and analysis of midgut proteinases. | seventy-eight bacillus thuringiensis isolates were selected for a screening against the lepidoptera species agrotis segetum to search the higher insecticidal activity. in a preliminary bioassay, the spore-crystal mixture of 78 b. thuringiensis isolates was tested against l1 larvae of a. segetum. fifty-two isolates had more than 60% corrected mortality after 3 days. seven isolates caused a corrected mortality of 100% on a. segetum. twelve isolates were selected for a second bioassay investigating ... | 2015 | 24839868 |
| agrotis segetum midgut putative receptor of bacillus thuringiensis vegetative insecticidal protein vip3aa16 differs from that of cry1ac toxin. | considering the fact that agrotis segetum is one of the most pathogenic insects to vegetables and cereals in the world, particularly in africa, the mode of action of vip3aa16 of bacillus thuringiensis bupm95 and cry1ac of the recombinant strain bns3cry-(phtcry1ac) has been examined in this crop pest. a. segetum proteases activated the vip3aa16 protoxin (90kda) yielding three bands of about 62, 45, 22kda and the activated form of the toxin was active against this pest with an lc50 of about 86ng/c ... | 2013 | 23876657 |
| betabaculovirus f proteins showed different efficiencies when rescuing the infectivity of gp64-null autographa californica nucleopolyhedrovirus. | the agrotis segetum granulovirus (agsegv) f protein was previously identified as the first betabaculovirus f protein with functional homology to autographa californica nucleopolyhedrovirus (acmnpv) gp64. in the current study, f proteins from xestia c-nigrum granulovirus (xecngv), cydia pomonella granulovirus (cpgv), phthorimaea operculella granulovirus (phopgv), choristoneura occidentalis granulovirus (chocgv) and plutella xylostella gv (plxygv) were studied for their ability to rescue the infec ... | 2013 | 23245471 |
| a moth pheromone brewery: production of (z)-11-hexadecenol by heterologous co-expression of two biosynthetic genes from a noctuid moth in a yeast cell factory. | moths (lepidoptera) are highly dependent on chemical communication to find a mate. compared to conventional unselective insecticides, synthetic pheromones have successfully served to lure male moths as a specific and environmentally friendly way to control important pest species. however, the chemical synthesis and purification of the sex pheromone components in large amounts is a difficult and costly task. the repertoire of enzymes involved in moth pheromone biosynthesis in insecta can be seen ... | 2013 | 24330839 |
| the origins of replication of granuloviruses. | the genomes of eight granuloviruses (gvs), have been analyzed for the presence of homologous regions (hrs) that may act as origins of replication. thirteen 74-76-bp palindromes within 11 hrs have previously been identified in the cydia pomonella gv (cpgv) genome and found to replicate in an infection-dependent dna replication assay. we report a further palindrome within one of the hrs, which was found to replicate, bringing the total to 14 palindromes. we also report imperfect palindromes, with ... | 2008 | 18612587 |
| stable isotope signatures reflect dietary diversity in european forest moths. | information on larval diet of many holometabolous insects remains incomplete. carbon (c) and nitrogen (n) stable isotope analysis in adult wing tissue can provide an efficient tool to infer such trophic relationships. the present study examines whether moth feeding guild affiliations taken from literature are reflected in isotopic signatures. | 2016 | 27555876 |
| receptor for detection of a type ii sex pheromone in the winter moth operophtera brumata. | how signal diversity evolves under stabilizing selection in a pheromone-based mate recognition system is a conundrum. female moths produce two major types of sex pheromones, i.e., long-chain acetates, alcohols and aldehydes (type i) and polyenic hydrocarbons and epoxides (type ii), along different biosynthetic pathways. little is known on how male pheromone receptor (pr) genes evolved to perceive the different pheromones. we report the identification of the first pr tuned to type ii pheromones, ... | 2016 | 26729427 |
| annual migration of agrotis segetum (lepidoptera: noctuidae): observed on a small isolated island in northern china. | migration behavior of the turnip moth, agrotis segetum (lepidoptera: noctuidae), is not well known by far. here, we present the data from an 11-year study on a. segetum by means of searchlight trapping and ovarian dissection on beihuang (bh) island, which located in the center of the bohai strait in northern china. the data showed a large number of a. segetum flight across the strait each year, which provides direct evidence that a. segetum is a long-distance migrant, migrating at least 40-60 km ... | 2015 | 26114576 |
| a distinct group ii alphabaculovirus isolated from a peridroma species. | the genome sequence of an alphabaculovirus isolated from a peridroma species indicated that it is a novel member of a group ii lineage most closely related to alphabaculoviruses from spodoptera exigua and agrotis segetum. it contains a genome of 151,110 nucleotides (nt), with a g+c content of 53.3%. | 2015 | 25838477 |
| mortality of cutworm larvae is not enhanced by agrotis segetum granulovirus and agrotis segetum nucleopolyhedrovirus b coinfection relative to single infection by either virus. | mixed infections of insect larvae with different baculoviruses are occasionally found. they are of interest from an evolutionary as well as from a practical point of view when baculoviruses are applied as biocontrol agents. here, we report mixed-infection studies of neonate larvae of the common cutworm, agrotis segetum, with two baculoviruses, agrotis segetum nucleopolyhedrovirus b (agsenpv-b) and agrotis segetum granulovirus (agsegv). by applying quantitative pcr (qpcr) analysis, coinfections o ... | 2015 | 25681187 |
| first record of steinernema kraussei (rhabditida: steinernematidae) from turkey and its virulence against agrotis segetum (lepidoptera: noctuidae). | during a survey of entomopathogenic nematodes (epns) in the eastern black sea region of turkey in 2009-2012, a steinernematid species was recorded and isolated using the galleria-baiting method. the isolate was identified as steinernema kraussei based on its morphological and molecular properties. the analysis of the its rdna sequence placed the turkish population of s. kraussei in the "feltiae-kraussei" group in the clade that contains different isolates of the species. this is the first record ... | 2013 | 24379483 |
| functional evolution of a multigene family: orthologous and paralogous pheromone receptor genes in the turnip moth, agrotis segetum. | lepidopteran pheromone receptors (prs), for which orthologies are evident among closely related species, provide an intriguing example of gene family evolution in terms of how new functions may arise. however, only a limited number of prs have been functionally characterized so far and thus evolutionary scenarios suffer from elements of speculation. in this study we investigated the turnip moth agrotis segetum, in which female moths produce a mixture of chemically related pheromone components th ... | 2013 | 24130875 |
| the complete mitogenome of the turnip moth agrotis segetum (lepidoptera: noctuidae). | abstract the complete mitochondrial genome (mitogenome) of the turnip moth agrotis segetum (lepidoptera: noctuidae) was determined (genbank accession no. kc894725). this is the first sequenced mitogenome from the subfamily noctuinae of noctuidae. the length of this mitogenome is 15,378 bp with a a+t content of 80.7%. there are 37 typical animal mitochondrial genes and a a+t-rich region. the trna gene trnm was the only rearranged gene compared with the pupative ancestral arrangement of insects. a ... | 2014 | 23795842 |
| [expression and synergistic function of enhancin-like gene of agrotis segetum granulovirus]. | enhancin is an enhancing protein chiefly found in insect baculoviruses. one enhancin homologue was identified, by blast method, in agrotis segetum granulovirus (agsegv) genome, named enhancin-like. sequence analysis indicated that this gene includes the conserved domains, conserved in other enhancin, and it has no signal peptide or a-transmembrane helix. a proline-rich domain, which is similar to those of mammals, is present at its c-terminal. to analyze the synergistic function of agsegv enhanc ... | 2012 | 22764529 |
| male and female noctuid moths attracted to synthetic lures in europe. | in field tests in europe, traps baited with a blend of isoamyl alcohol, acetic acid, and isobutanol (compounds previously found attractive to a number of noctuids in north america) caught the following noctuid moths: agrotis segetum schiff., agrotis crassa hbn., agrotis exclamationis l., amathes (xestia) c-nigrum l., apatele rumicis l., amphipyra pyramidea l., dipterygia scabriuscula l., discestra trifolii hfn., euxoa aquilina schiff., euclidia glyphica l., mamestra brassicae l., mamestra olerac ... | 2010 | 20449640 |
| global transcriptional analysis of pheromone biosynthesis-related genes in the female turnip moth, agrotis segetum (noctuidae) using a custom-made cdna microarray. | using a custom-made cdna microarray, global transcriptional analyses were conducted to identify genes differentially regulated in the pheromone gland as compared to the remaining insect tissue of the moth agrotis segetum (noctuidae). a two-fold or larger difference in relative expression levels was found for 227 of 864 genes investigated comparing the two tissues. unexpectedly, an antennal binding protein homologue, containing a pheromone-binding/general odorant-binding protein pfam domain, was ... | 2009 | 19376228 |
| the release of a pheromonotropic neuropeptide, pban, in the turnip moth agrotis segetum, exhibits a circadian rhythm. | in the female turnip moth, agrotis segetum, a pheromone biosynthesis activating neuropeptide (pban) stimulates sex pheromone biosynthesis which exhibits a daily rhythm. here we show data supporting a circadian rhythm in pban release from the corpora cardiaca, which we propose regulates the endogenous rhythm in sex pheromone biosynthesis. this conclusion is drawn as the observed daily rhythm in pban-like immunoreactivity in the hemolymph is persistent in constant darkness and is phase-shifted by ... | 2009 | 19041654 |
| transcriptional analysis of the pheromone gland of the turnip moth, agrotis segetum (noctuidae), reveals candidate genes involved in pheromone production. | moths generally rely on pheromone communication for mate finding. the pheromone components of most moths are produced by a common pathway of fatty-acid biosynthesis coupled with species-specific modifications of the final products. some genes involved in moth pheromone production have previously been described, whereas others remain to be characterized and thus the molecular mechanisms accounting for the production of species-specific blends are far from understood. the turnip moth, agrotis sege ... | 2008 | 18237286 |
| appetitive flight patterns of male agrotis segetum moths over landscape scales. | an analysis is presented of the first harmonic radar studies of pheromone-plume locating flights of male agrotis segetum moths over distances of up to 500 m. upon release most moths flew in a direction having a downwind component. the first significant changes in flight orientations occur in the immediate vicinity of a pheromone source. moths that were initially flying downwind change course and start flying crosswind whilst those that initially flew crosswind change course and start flying upwi ... | 2007 | 17109897 |
| the circadian rhythm of the sex-pheromone-mediated behavioral response in the turnip moth, agrotis segetum, is not controlled at the peripheral level. | the pheromone-mediated upwind flight of male turnip moths was observed in a flight tunnel at different times of day under conditions of a light-dark (ld) cycle, constant darkness (dd), and a shifted photoperiod. under both ld and dd conditions, a significantly larger number of males flew to the pheromone during both the scotophase and the subjective scotophase than during the photophase and the subjective photophase for 2 consecutive days. when 1-day-old moths were transferred to a shifted ld cy ... | 2003 | 14582856 |
| responses in highly selective sensory neurons to blends of pheromone components in the moth agrotis segetum. | pheromone detecting sensory neurons in moths are known to be highly sensitive and selective. female-emitted sexual pheromones are normally mixtures of a few to several components. however, not much is known about how receptor neurons respond to blends of compounds. in the present study we investigated how four physiological types of pheromone component-selective neurons responded to binary mixtures or to the complete blend in the turnip moth agrotis segetum. we found that responses to mixtures o ... | 2002 | 12770093 |
| entomopoxvirus of cotton bollworm, helicoverpa armigera (hbn.). | occurrence of an entomopoxvirus (epv) from a lepidopteran insect viz;. cotton bollworm, h. armigera (haepv) along with gross pathological symptoms is reported for the first time in india. histopathological study revealed that the fat body being the most favoured site of infection followed by haemocytes and gut epithelium. haepv was found to be not cross infective to six of the agricultural lepidopteran insect pests except for the potato black cutworm, agrotis segetum registering 100% mortality s ... | 2002 | 12597557 |
| endogenous control of circadian rhythms of pheromone production in the turnip moth, agrotis segetum. | the circadian variation of pheromone production in the turnip moth, agrotis segetum, was characterized by quantifying (z)-7-dodecenyl acetate (z7-12:oac), the most abundant pheromone component produced by female turnip moth, at different times of day. under 17:7 h light-dark cycle (ld), the peak of z7-12:oac production occurred around 4 h into the scotophase, while there was very little pheromone production during the photophase. when females were maintained under constant darkness (dd), the per ... | 2002 | 11948972 |
| olfactory protocerebral pathways processing sex pheromone and plant odor information in the male moth agrotis segetum. | we investigated protocerebral processing of behaviorally relevant signals in the turnip moth, agrotis segetum. single neurons were studied both physiologically and morphologically using intracellular recording techniques. in moth pheromone communication systems, the presence of the complete, female-produced pheromone blend is necessary for male attraction. we predicted that more protocerebral neurons, compared with al, would display blend interactions. however, only a few protocerebral neurons r ... | 2001 | 11246213 |
| temperature-induced pupil movements in insect superposition eyes. | in this paper, we describe the hitherto largely overlooked effect of temperature on the pupil of insect compound eyes. in the turnip moth agrotis segetum and in two other nocturnal insects with superposition eyes, the lacewing euroleon nostras and the codling moth cydia pomonella, the pupil not only opens and closes with changes in the ambient light level, as expected, but also with changes in temperature in the absence of light. in complete darkness, the pupil of a. segetum responds over a wide ... | 2000 | 10648210 |
| intraspecific nucleotide variation at the pheromone binding protein locus in the turnip moth, agrotis segetum. | inter- and intraspecific amino acid variability in the pheromone binding proteins (pbps) of the lepidoptera is believed to contribute to a molecular mechanism of pheromone blend discrimination. messenger rna coding for pbp sequence in agrotis segetum (noctuidae) was cloned, and nucleotide and inferred amino acid variation across a 769-bp region of a pbp locus was studied in two populations. a single gene copy was fully sequenced, revealing an intron/exon structure conserved with distant saturnii ... | 1999 | 10620043 |
| central processing of pulsed pheromone signals by antennal lobe neurons in the male moth agrotis segetum. | male moths use female-produced pheromones as orientation cues during the mate-finding process. in addition to the needs of evaluating the quality and quantity of the pheromone signal, the male moth also needs to resolve the filamentous structure of the pheromone plume to proceed toward the releasing point successfully. to understand how a discontinuous olfactory signal is processed at the central level, we used intracellular recording methods to characterize the response patterns of antennal lob ... | 1999 | 10085338 |
| enantiomers of cis- and trans-3-(4-propyl-cyclopent-2-enyl) propyl acetate. a study on the bioactive conformation and chiral recognition of a moth sex pheromone component. | the enantiomers of cis- and trans-3-(4-propyl-cyclopent-2-enyl) propyl acetate, which are conformationally constrained analogues of (z)-5-decenyl acetate (1), a sex pheromone component of the turnip moth, agrotis segetum, have been synthesized and tested using the electrophysiological single-sensillum technique. the analogues mimic a cisoid and transoid conformation of 1, respectively. in addition, the enantiomers of each of the cis- and trans-isomers are conformationally constrained analogues o ... | 1997 | 9459015 |
| discrimination among pheromone component blends by interneurons in male antennal lobes of two populations of the turnip moth, agrotis segetum. | a difference in female pheromone production and male behavioral response has previously been found in two populations of the turnip moth, agrotis segetum, originating from sweden and zimbabwe, respectively. in this study, we investigated the pheromone response of antennal lobe interneurons of males of the two populations by intracellular recordings, stimulating with single pheromone components and various inter- and intra-populational pheromone blends. three major physiological types of antennal ... | 1996 | 8755596 |
| conformationally constrained analogues of (z)-5-decenyl acetate, a pheromone component of agrotis segetum. | conformationally constrained analogues of (z)-5-decenyl acetate (1), a pheromone component of the turnip moth, agrotis segetum, have been synthesized and tested by using electrophysiological single-cell recordings. in the constrained analogues the terminal alkyl chain in 1 has been incorporated in a six-membered (3 and 4) or five-membered (6) ring system. these cycli compounds are also conformationally constrained analogues of the previously deduced bioactive conformations of the corresponding c ... | 1996 | 8733633 |
| ab initio calculations on (z)-5-decenyl acetate, a component of the pheromone complex of agrotis segetum (lepidoptera: noctuidae) and electrophysiological studies with chain elongated analogues. | conformational analyses of (z)-5-decenylacetate, a sex pheromone component of the turnip moth, agrotis segetum, and double unsaturated pheromone analogues 4 and 5 have been performed by ab initio calculations using gaussian 92. two minima were found for a cisoid and a transoid conformer, differing for 0.03 kcal/mol only. conformational energies of diene analogues (5z,7e)-5,7-decadienyl acetate (4) and (3e,5z)-3,5,-decadienyl acetate (5) were determined for conformers required to mimic spatial re ... | 1996 | 8733629 |
| the peripheral auditory characteristics of noctuid moths: responses to the search-phase echolocation calls of bats | the noctuid moths agrotis segetum and noctua pronuba show peak auditory sensitivity between 15 and 25 khz, and a maximum sensitivity of 35 db spl. a. segetum shows a temporal integration time of 69 ms. it is predicted that bats using high-frequency and short-duration calls will be acoustically less apparent to these moths. short-duration frequency-modulated (fm) calls of plecotus auritus are not significantly less acoustically apparent than those of other fm bats with slightly longer call durati ... | 1996 | 9318627 |
| migration of neurons between ganglia in the metamorphosing insect nervous system. | migration of neurons over long distances occurs during the development of the adult central nervous system of the sphinx moth manduca sexta, and the turnip moth agrotis segetum. from each of the suboesophageal and three thoracic ganglia, bilaterally-paired clusters of immature neurons and associated glial cells migrate posteriorly along the interganglionic connectives, to enter the next posterior ganglion. the first sign of migration is observed at the onset of metamorphosis, when posterio-later ... | 1995 | 28306061 |
| how is pheromone specificity encoded in proteins? | pheromone specificity in the lepidoptera is encoded in protein components of the antennal sensillum lymph and dendritic membrane. in this paper, we highlight recent work on the molecular determinants of pheromone binding affinity of pheromone binding proteins (pbps) of three genera. first, we describe new cdna sequences for lymantria dispar (lymantriidae) and agrotis segetum (noctuidae). these data enrich the conclusions derived from our functional studies. secondly, we indicate how preparation ... | 1995 | 8590031 |
| alkyl ether and enol ether analogs of (z)-5-decenyl acetate, a pheromone component of the turnip moth,agrotis segetum: probing a proposed bioactive conformation for chain-elongated analogs. | in order to test a previous conclusion that chain-elongated analogs of (z)-5-decenyl acetate(1), a pheromone component of the turnip moth,agrotis segetum, adopt a loop conformation of the terminal alkyl chain in the bioactive conformation, a series of alkyl ether and enol ether analogs of1 and (z)-5-dodecenyl acetate(2) have been synthesized and tested using singlecell electrophysiology. in these analogs a methylene group in positions 7 and 9 of1 and in positions 7 and 11 in2 have been replaced ... | 1995 | 24234320 |
| oxime ether analogs of sex pheromone components of turnip moth (agrotis segetum schiffermüller). | oxime ether analogs of sex pheromone components of the turnip moth (agrotis segetum schiff.) were synthesized by the acidolytic opening of cyclic enol ethers witho-alkyl hydroxylamine hydrochlorides. the compounds varying in chain lengths and in the position of the c=n double bond were studied by electrophysiological single sensillum recordings (electrosen-sillography: esg). the esg data indicate in general reduced receptor interaction of all analogs investigated in comparison with natural phero ... | 1994 | 24242304 |
| characterization of new baculovirus genotypes arising from inoculation of pieris brassicae with granulosis viruses. | previous studies have shown that of 15 artogeia (pieris) rapae granulosis virus isolates (argv1 to argv15) only two, argv1 and argv2, gave a normal dose-mortality response in larvae from an established colony of pieris brassicae. we report here that at extremely high doses, approaching 10000 times the ld50 for argv1 and argv2, three other argv isolates caused low and irregular levels of mortality in p. brassicae. at similar doses agrotis segetum gv caused 43% mortality in one infection, but no d ... | 1993 | 8445365 |
| electrophysiological and behavioral responses of turnip moth males,agrotis segetum to fluorinated pheromone analogs. | the electrophysiological and behavioral responses of maleagrotis segetum to fluorinated analogs of (z)-5-decenyl acetate (z5-10∶oac) were investigated. the single sensillum recordings showed that 4,4-difluoro-(z)-5-decenyl acetate (4,4-f2), 7,7-difluoro-(z)-5-decenyl acetate (7,7-f2), 10,10,10-trifluoro-(z)-5-decenyl acetate (10,10,10-f3) and 7,7,8,8-tetrafluoro-(z)-5-decenyl acetate (7,7,8,8-f4) were each 100-fold less active than the natural z5-10 ∶ oac, whereas the 7,7,8,8,9,9,10,10,10-nonafl ... | 1993 | 24248519 |
| postembryonic development of leucokinin i-immunoreactive neurons innervating a neurohemal organ in the turnip moth agrotis segetum. | in the abdominal ganglia of the turnip moth agrotis segetum, an antibody against the cockroach neuropeptide leucokinin i recognizes neurons with varicose fibers and terminals innervating the perisympathetic neurohemal organs. in the larva, the abdominal perisympathetic organs consist of a segmental series of discrete neurohemal swellings on the dorsal unpaired nerve and the transverse nerves originating at its bifurcation. these neurohemal structures are innervated by varicose terminals of leuco ... | 1992 | 1423486 |
| functional specialization of olfactory glomeruli in a moth. | the specific function of the glomerular structures present in the antennal lobes or olfactory bulbs of organisms ranging from insects to humans has been obscure because of limitations in neuronal marking methods. by tracing individual neurons in the moth agrotis segetum, it was determined that physiologically distinct types of pheromone receptor neurons project axons to different regions of the macroglomerular complex (mgc). each glomerulus making up the mgc has a specific functional identity, i ... | 1992 | 1598574 |
| introduction of methyl groups to acetate substituted chain of (z)-5-decenyl acetate, a pheromone component of turnip moth,agrotis segetum: synthesis, single-sensillum recordings, and structure-activity relationships. | methyl-substituted analogs of (z)-5-decenyl acetate, a pheromone component of the turnip moth,agrotis segetum, have been synthesized and studied by electrophysiological single-sensillum recordings and molecular mechanics calculations [mm2(85)]. the analogs are monomethyl substituted in the 2, 3, 4, and 5 positions and geminally dimethyl substituted in the 2, 3, and 4 positions. the methyl groups have been employed as space probes to study the degree of steric complementarity between the acetate- ... | 1992 | 24253872 |
| response of steinernema carpocapsae infective juveniles to the plasma of three insect species. | exsheathed infective juveniles of steinernema carpocapsae all strain were attracted to the plasma of three species of insects in agar plate bioassays. plasma of pieris rapae crucivora, spodoptera litura, and agrotis segetum attracted 88.6%, 80.4%, and 64.4%, respectively, of steinernema carpocapsae juveniles added to plates. autoclaved plasma of s. litura larvae attracted more juveniles than saline controls, but less than nonautoclaved plasma. the active agent passed through a 14,000 mw dialysis ... | 1992 | 19283217 |
| replacement of the terminal methyl group in a moth sex pheromone component by a halogen atom: hydrophobicity and size effects on electrophysiological single-cell activities. | analogs of (z)-5-decenyl acetate, a pheromone component of the turnip moth,agrotis segetum, with a chloro, bromo, or iodo substituent in place of the terminal methyl group have been synthesized and tested by electrophysiological single-sensillum recordings. the electrophysiological results have been interpreted in terms of substituent size and hydrophobicity. interaction energies based on receptor interaction models have been calculated using the molecular mechanics [mm2(85)] method. the results ... | 1991 | 24257799 |
| alkyl substitution in terminal chain of (z)-5-decenyl acetate, a pheromone component of turnip moth,agrotis segetum. synthesis, single-sensillum recordings, and structure-activity relationships. | structure-activity relationships for 6-, 7-, 8-, and 9-alkyl substituted analogs of (z)-5-decenyl acetate, a pheromone component of the turnip moth,agrotis segetum, have been studied by electrophysiological single-sensillum recordings, and interpreted in terms of a receptor-interaction model. the compounds were prepared by alkenyl cuprate reactions withα,β-unsaturated carbonyl derivatives or alkyl halides. the electrophysiological results indicate steric repulsive interactions between the alkyl ... | 1991 | 24258437 |
| [primary structure of granulin of the granulosis virus of agrotis segetum]. | | 1990 | 2090117 |
| structure-activity relationships for chain-shortened analogs of (z)-5-decenyl acetate, a pheromone component of the turnip moth,agrotis segetum. | structure-activity relationships for chain-shortened analogs of (z)-5-decenyl acetate, a pheromone component of the turnip moth,agrotis segetum, have been studied by electrophysiological single-sensillum technique and interpreted in terms of a previously reported receptor-interaction model. the results indicate that the terminal methyl group, as well as the acetate group, interacts with highly complementary receptor sites. the terminal alkyl chain is suggested to interact with a hydrophobic "poc ... | 1990 | 24263583 |
| effects of double-bond configuration on interaction between a moth sex pheromone component and its receptor : a receptor-interaction model based on molecular mechanics. | the dependence of the electrophysiological activity on the change of double-bond configuration of (z)-5-decenyl acetate, a pheromone component of the turnip moth,agrotis segetum, and a dienic analog, (e)-2,(z)-5-decadienyl acetate, have been investigated by single-cell measurements and molecular mechanics calculations (mm2). a previously reported model for the interaction between a moth sex pheromone component and its receptor has been refined. this new model gives an essentially quantitative co ... | 1987 | 24301472 |
| novel fraction collector for studying the oviposition rhythm in the turnip moth. | turnip moths, agrotis segetum (schiff.) (lepidoptera: noctuidae), were held in a transparent plastic box with a window opening to a rotating paper drum upon which the females could lay eggs. a novel fraction collector, consisting of a standard 24-hr wall timer and simple electronic circuit, served to rotate the paper in hourly increments. the entire apparatus was housed in an environmental chamber on a 16 hr light: 8 hr dark photoperiod at constant 23.7 degrees c and 55% r.h. under these conditi ... | 1987 | 3508740 |
| behavioral responses of male turnip moths,agrotis segetum, to sex pheromone in a flight tunnel and in the field. | the response of individual male turnip mothsagrotis segetum was observed in a sustained flight tunnel to a mixture of decyl acetate, (z)-5-decenyl acetate, (z)-7-dodecenyl acetate, and (z)-9-tetradecenyl acetate in proportions similar to those found in gland extracts from virgin females (0.6: 1:5:2.5). lures containing 3-30 μg (z)-5-decenyl acetate proved to be maximally attractive, with approximately 60% of the males completing all behavioral steps from activation to copulation efforts. a 300-μ ... | 1985 | 24310383 |
| individual variation in the pheromone of the turnip moth,agrotis segetum. | female turnip moths (agrotis segetum) from a laboratory culture inbred for more than 30 generations, and the offspring (first and third generation) from field-collected insects were analyzed individually for acetates and alcohols in the pheromone gland. quantitative analysis of individual components was performed at the subnanogram level by gas chromatographymass spectrometry (selected ion monitoring). the titer of the pheromone, i.e., the sum of the homologous acetates (z)-5-decenyl acetate, (z ... | 1985 | 24310381 |
| structure-activity relationships between stimulus molecule and response of a pheromone receptor cell in turnip moth,agrotis segetum : modifications of the acetate group. | the response of an antennal receptor cell of the turnip moth,agrotis segetum, was recorded during stimulation with a series of (z)-7-dodecenyl acetate analogs with structural variations of the acetate group. the investigated receptor cell is known to be highly selective to (z)-7-dodecenyl acetate. all parts of the acetate group were found to be of great importance for full biological activity. the results indicate very strict requirements on the shape of the polar functional group, as well as on ... | 1984 | 24318425 |
| determination of the vapor pressures of moth sex pheromone components by a gas chromatographic method. | the vapor pressures of decyl acetate, five decenyl acetate isomers, (z)-7-dodecenyl acetate, and (z)-9-tetradecenyl acetate have been determined at three to six temperatures in the interval 25-45 °c by a gas chromatographic method suitable for accurate measurements of the low vapor pressures of moth sex pheromone components at biologically relevant temperatures. the vapor pressure values at 30.5 °c are 3.80 pa for decyl acetate, 4.08-5.40 pa for the decenyl acetate isomers, 0.562 pa for (z)-7-do ... | 1983 | 24407406 |
| [dna of the polyhedras and granules of the turnip moth agrotis segetum schiff]. | the properties of dna of nuclear polyhedrosis (npv) and granulosis (gv) viruses of turnip moth, agrotis segetum schiff were studied. these dnas were found to differ in all their properties. npv dna belongs to the gc-type, gv dna to at type: the content of gc-pairs for npv was 53.6% for gv 40.7%, the melting temperature in i ssc was 91.3 degrees c and 86.6 degrees c, respectively. the molecular weight of dna first determined by 4 independent methods (electron microscopy, restriction analysis, zon ... | 2016 | 6346688 |
| a biochemical and biological comparison of three european isolates of nuclear polyhedrosis viruses from agrotis segetum. | isolates of multiply-enveloped nuclear polyhedrosis viruses from agrotis segetum (lepidoptera: noctuidae) populations in england (asnpve), france (asnpvf) and poland (asnpvp), were compared biochemically and for their infectivity to a. segetum and mamestra brassicae larvae. the electrophoretic profiles of dna restriction endonuclease fragments and viral proteins appeared identical for asnpve and asnpvf. asnpvp was distinct by these techniques, although some of the virus particle polypeptides had ... | 1983 | 6299246 |
| effect of gamma radiation on potato cutworm agrotis segetum schiffer-mueller. | | 1982 | 7170334 |
| sex pheromone components of the turnip moth,agrotis segetum : chemical identification, electrophysiological evaluation and behavioral activity. | analysis of female abdominal tips ofagrotis segetum by means of gc-ms showed the presence of 13 aliphatic acetates and alcohols. (z)-7-dodecenyl acetate was found to be the main component in the extracts at amounts of about 1 ng/female. (z)-9-tetradecenyl acetate and (z)-7-dodecenol were present to the extent of 49 and 19%, respectively, of the main component. minor components could be identified as decyl acetate, (z)-5-decenyl acetate, dodecyl acetate, (z)-9-dodecenyl acetate, tetradecyl acetat ... | 1982 | 24414737 |
| [dna packing inside viral particles of agrotis segetum (schiff) turnip moth granulosis virus]. | the structure of high molecular (88 million daltons) dna of turnip moth granulosis virus obtained in the linear and cyclic forms was studied. electron microscopic examination of the fine structure of dna isolated from virus particles treated with n-toluene sulphonate-n-cyclohexyl-n'-beta-4 (methyl morpholine)-ethylcarbodiimide were carried out. modified dna areas were visualized by two methods: with p32 protein and phosphotungstate acid. in both cases a period of recurrence close to the length o ... | 2016 | 7257315 |
| fine-structural characteristics of the antennal sensilla of agrotis segetum (insecta: lepidoptera). | the turnip moth agrotis segetum possesses seven different types of sensilla: four single-walled (sw), one double-walled (dw), one terminal-pore (tp), and one poreless sensilla (np). the sw 1 and sw 2 sensilla have the same external appearance, being long and slender, but differ in the branching pattern of the sensory processes: unbranched and branched in sw 1 and sw 2, respectively. the sw 3 sensilla are shorter, sickle-shaped, and contain a large number of branches from the sensory processes. t ... | 1981 | 7249062 |
| [pathogenic bacterial flora of the winter cutworm (agrotis segetum schiff)]. | | 2003 | 4783474 |
| [the packing of dna in the virus particles of agrotis segetum (schiff.) granules]. | | 2016 | 5086851 |
| [the bacterial flora of healthy agrotis segetum schiff in various developmental stages]. | | 2000 | 5085877 |
| [study of (agrotis segetum schiff. l.) in the microflora forest steppe of the ukraine]. | | 1972 | 4663639 |
| electron microscope investigation of granulosis viruses of dendrolimus sibiricus and agrotis segetum. | | 1971 | 5092828 |
| [histochemical and cytophotometric study of neuro-secretory cells of the dart-moth agrotis segetum schiff with special consideration of the ddd reaction]. | | 1966 | 4172418 |