| rt-pcr using redundant primers to detect the three viruses associated with carrot motley dwarf disease. | a method was developed for detecting and distinguishing the viruses associated with carrot motley dwarf (cmd) disease, i.e. carrot mottle umbravirus (cmov), carrot red leaf virus (crlv) and the virus known as carrot red leaf virus-associated rna (crlvarna). redundant primers were made that targeted the rna-dependent rna polymerase (rdrp) gene in all available sequences of umbraviruses and in a subset of polerovirus genomes, and specific and redundant primers were made to target the same gene in ... | 2000 | 10960703 |
| the genome organization and affinities of an australian isolate of carrot mottle umbravirus. | the genomic sequence of an australian isolate of carrot mottle umbravirus (cmov-a) was determined from cdna generated from dsrna. this provides the first data on the genome organization and phylogeny of an umbravirus. the 4201-nucleotide genome contains four major open reading frames (orfs). analysis suggests that orf2 encodes an rna-dependent rna polymerase, that orf4 encodes a movement protein, and that the virus has no coat protein gene. the functions of orfs 1 and 3 remain unknown. orf2 is p ... | 1996 | 8862426 |
| nucleotide sequence of a satellite rna associated with carrot motley dwarf in parsley and carrot. | carrot motley dwarf (cmd) is known to result from a mixed infection by two viruses, the polerovirus carrot red leaf virus and one of the umbraviruses carrot mottle mimic virus or carrot mottle virus. some umbraviruses have been shown to be associated with small satellite (sat) rnas, but none have been reported for the latter two. a cmd-affected parsley plant was used for sap transmission to test plants, that were used for dsrna isolation. the presence of a 0.8-kbp dsrna indicated the occurrence ... | 2009 | 19020967 |