| some unusual members of the family pasteurellaceae isolated from human sources--phenotypic features and genomic relationships. | twelve human isolates exhibiting the properties of the family pasteurellaceae but phenotypically deviating from established species, or resembling species of animal origin that are only rarely reported to occur in human materials, were checked for their identities by dna-dna hybridization. the collection consisted of one strain of actinobacillus lignieresii, two strains of actinobacillus hominis (mannose-positive), two hitherto undescribed actinobacillus or actinobacillus-like species, [pasteure ... | 1991 | 1930571 |
| actinobacillus rossii sp. nov., actinobacillus seminis sp. nov., nom. rev., pasteurella bettii sp. nov., pasteurella lymphangitidis sp. nov., pasteurella mairi sp. nov., and pasteurella trehalosi sp. nov. | evidence from numerical taxonomic analysis and dna-dna hybridization supports the proposal of new species in the genera actinobacillus and pasteurella. the following new species are proposed: actinobacillus rossii sp. nov., from the vaginas of postparturient sows; actinobacillus seminis sp. nov., nom. rev., associated with epididymitis of sheep; pasteurella bettii sp. nov., associated with human bartholin gland abscess and finger infections; pasteurella lymphangitidis sp. nov. (the blg group), w ... | 1990 | 2223608 |
| a minimal medium for growth of pasteurella multocida. | we developed a minimal medium supporting the growth of both toxigenic and nontoxigenic strains of pasteurella multocida to optical densities of > 0.5 (600 nm). p. multocida p1059 (atcc 15742), one of a number of strains which can cause fowl cholera, was used as the model strain in this study. the medium was composed of 17 ingredients including cysteine, glutamic acid, leucine, methionine, inorganic salts, nicotinamide, pantothenate, thiamine, and an energy source. leucine was not required for gr ... | 1996 | 8764479 |
| phylogenetic relationships and diversity within the pasteurella haemolytica complex based on 16s rrna sequence comparison and outer membrane protein and lipopolysaccharide analysis. | the outer membrane protein (omp) and lipopolysaccharide (lps) profiles of 30 untypeable isolates of pasteurella haemolytica were examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and compared with the profiles of typeable isolates. the phylogenetic relationships of 28 isolates representing each of the serotypes of p. haemolytica and pasteurella trehalosi, as well as untypeable isolates of p. haemolytica, were determined by comparing 16s rrna sequences. the analysis of the omp ... | 1996 | 8782683 |
| intra-specific diversity within pasteurella trehalosi based on variation of capsular polysaccharide, lipopolysaccharide and outer-membrane proteins. | intra-specific diversity within pasteurella trehalosi was investigated by analysis of variation of capsular polysaccharide, and lipopolysaccharide (lps) and outer-membrane protein (omp) profiles. sixty isolates of p. trehalosi, from diverse geographical locations within the uk, were examined. capsular polysaccharide serotypes were determined by indirect haemagglutination assay; lps and omp profiles were compared by sds-page analysis. capsular serotyping identified three isolates of serotype t3, ... | 1996 | 8868430 |
| the isolation of recombinant plasmids expressing secreted antigens of pasteurella haemolytica a1 and the characterization of an immunogenic 60 kda antigen. | a collection of recombinant plasmids had been isolated which express secreted antigens of pasteurella haemolytica a1 likely to be important in protection. the recombinant escherichia coli clones were found to express the p. haemolytica a1 antigens by western immunoblot analysis, using sera from calves which had been vaccinated with presponse and were subsequently resistant to experimental challenge with p. haemolytica a1. detailed analysis of three of the recombinant plasmids (pgs1-17, pgs3-19 a ... | 1996 | 8870198 |
| divergent activity and function of superoxide dismutases in pasteurella haemolytica serotypes a1 and a2 and pasteurella trehalosi serotype t10. | representative strains of pasteurella haemolytica serotypes a1 and a2 and pasteurella trehalosi serotype t10 were examined for the presence of superoxide dismutase. visualisation of superoxide dismutase enzyme activity on polyacrylamide gels, and specific inhibition with potassium cyanide verified a copper/zinc (cu/zn) superoxide dismutase only in serotype a2 whereas serotypes a1 and t10 showed other superoxide dismutase activity. using a simple freeze-thaw method the cellular location of supero ... | 1997 | 9170262 |
| genetic relationships among pasteurella trehalosi isolates based on multilocus enzyme electrophoresis. | genetic diversity among 60 british pasteurella trehalosi isolates representing the four recognized capsular serotypes, t3, t4, t10 and t15, and recovered predominantly from sheep suffering from systemic pasteurellosis, was estimated by analysing electrophoretically demonstrable allelic variation at structural genes encoding 19 enzymes. thirteen of the locl were polymorphic and 20 distinctive multilocus genotypes (electrophoretic types, ets) were identified. the population structure of p. trehalo ... | 1997 | 9274038 |
| characterization of a cacag pentanucleotide repeat in pasteurella haemolytica and its possible role in modulation of a novel type iii restriction-modification system. | in a previous study, a recombinant plasmid that contains a cacag pentanucleotide repeat was isolated from a pasteurella haemolytica a1 library. southern hybridization analysis using a (cacag)5probe indicated the presence of two loci that contain the pentanucleotide repeats on the genome of p.haemolytica a1. additional hybridization analyses against genomic dna from related microorganisms indicated that the repeats are only present in p.haemolytica and pasteurella trehalosi t3. the various seroty ... | 1999 | 10037813 |
| polymerase chain reaction techniques for differentiating cytotoxic and noncytotoxic pasteurella trehalosi from rocky mountain bighorn sheep. | to evaluate 2 polymerase chain reaction (pcr)-based methods for differentiating cytotoxic and noncytotoxic pasteurella trehalosi from rocky mountain bighorn sheep (ovis canadensis canadensis). | 1999 | 10328428 |
| serotypes of pasteurella haemolytica and pasteurella trehalosi isolated from farm animals in hungary. | the biochemical and serological characteristics of 486 p. haemolytica and 31 p. trehalosi strains (517 in total) isolated from different lesions of cattle, sheep, goats, pigs and poultry were examined. a total of 476 p. haemolytica strains (97.9%) showed the characteristics typical of the former biotype a of p. haemolytica, while 10 isolates (2.1%), all from poultry, could not be biotyped. a total of 481 strains (93.0%) could be assigned to one of the 17 serotypes of p. haemolytica-p. trehalosi ... | 1999 | 10379234 |
| conservation of expression and n-terminal sequences of the pasteurella haemolytica 31-kilodalton and pasteurella trehalosi 29-kilodalton periplasmic iron-regulated proteins. | this study examined the conservation of expression of a 31-kda iron-regulated protein by serotypes of pasteurella haemolytica and pasteurella trehalosi associated with pasteurellosis of cattle and sheep. a polyclonal antibody prepared against the purified 31-kda periplasmic iron-regulated protein from p. haemolytica serotype a1 showed that all p. haemolytica serotypes expressed similar 31-kda proteins with identical n-terminal sequences, whereas p. trehalosi serotypes expressed immunologically d ... | 1999 | 10391874 |
| sequence diversity and molecular evolution of the leukotoxin (lkta) gene in bovine and ovine strains of mannheimia (pasteurella) haemolytica. | the molecular evolution of the leukotoxin structural gene (lkta) of mannheimia (pasteurella) haemolytica was investigated by nucleotide sequence comparison of lkta in 31 bovine and ovine strains representing the various evolutionary lineages and serotypes of the species. eight major allelic variants (1.4 to 15.7% nucleotide divergence) were identified; these have mosaic structures of varying degrees of complexity reflecting a history of horizontal gene transfer and extensive intragenic recombina ... | 2001 | 11157953 |
| seasonal incidence and antibiotic susceptibility patterns of pasteurellaceae isolated from american bison (bison bison). | ninety pharyngeal tonsils were collected from 2-year-old american bison (bison bison) bulls and sampled for members of the pasteurellaceae family. particular attention was paid to seasonal incidence and antimicrobial resistance in serotypes and biovariants. multiple strains of pasteurella haemolytica (39%), p. trehalosi (68%), p. multocida (34%) and haemophilus somnus (13%) were cultured from 86 out of the 90 (96%) tonsil samples. pasteurella trehalosi was the most common and evenly distributed ... | 2001 | 11227200 |
| evaluation of ewe vaccination as a tool for increasing bighorn lamb survival following pasteurellosis epizootics. | we conducted field and laboratory experiments to evaluate whether treating pregnant bighorn ewes with a combination of an experimental pasteurella trehalosi and mannheimia haemolytica (formerly p. haemolytica) vaccine and a commercially-available bovine p. multocida and m. haemolytica vaccine would increase lamb survival following a pneumonia epidemic. three free-ranging bighorn herds affected by pasteurellosis outbreaks between november 1995 and june 1996 were included in the field experiment. ... | 2001 | 11272504 |
| mosaic structure and molecular evolution of the leukotoxin operon (lktcabd) in mannheimia (pasteurella) haemolytica, mannheimia glucosida, and pasteurella trehalosi. | the mosaic structure and molecular evolution of the leukotoxin operon (lktcabd) was investigated by nucleotide sequence comparison of the lktc, lktb, and lktd genes in 23 mannheimia (pasteurella) haemolytica, 6 mannheimia glucosida, and 4 pasteurella trehalosi strains. sequence variation in the lkta gene has been described previously (r. l. davies et al., j. bacteriol. 183:1394-1404, 2001). the leukotoxin operon of m. haemolytica has a complex mosaic structure and has been derived by extensive i ... | 2002 | 11741868 |
| studies on the production of quorum-sensing signal molecules in mannheimia haemolytica a1 and other pasteurellaceae species. | the bioluminescence assay system using vibrio harveyi reporter strains were used to examine quorum-sensing autoinducer (ai) activity from mannheimia haemolytica a1 cell-free culture supernatant. we showed that m. haemolytica a1 cell-free culture supernatant contains molecules that can stimulate the quorum-sensing system that regulates the expression of the luciferase operon in v. harveyi. specifically, m. haemolytica a1 can stimulate only the quorum system 2 but not system 1, suggesting that the ... | 2002 | 11786252 |
| mannheimia haemolytica serotype 1 and pasteurella trehalosi serotype 10 culture supernatants contain fibrinogen-binding proteins. | fibrinogen-binding proteins were found in the culture supernatants of mannheimia haemolytica serotype 1 (atcc 43270) and pasteurella trehalosi serotype 10 (eco-100). sheep fibrinogen was biotinylated and shown to bind to proteins in the culture supernatants by modified western blot. fibrinogen-binding proteins in the culture supernatant may be important virulence factors leading to the characteristic fibrinous pneumonia caused by these organisms and may be critical antigenic targets for immune p ... | 2002 | 12406660 |
| survey of restriction-modification systems and transformation in mannheimia haemolytica and pasteurella trehalosi. | a significant obstacle to molecular studies of mannheimia (pasteurella) haemolytica, has been its resistance to genetic transformation. the lack of competence of many m. haemolytica strains has been attributed to the presence of restriction modification systems. in this study, representative strains of 12 m. haemolytica serotypes and four pasteurella trehalosi serotypes were successfully transformed by electroporation using a recombinant vector derived from the native m. haemolytica a1 serotype ... | 2003 | 12488074 |
| pcr-detection of hemophilus paragallinarum, hemophilus somnus, mannheimia (pasteurella) hemolytica, mannheimia spp., pasteurella trehalosi, and pasteurella multocida. | | 2003 | 12512370 |
| diversity of mannheimia haemolytica and pasteurella trehalosi serotypes from apparently healthy sheep and abattoir specimens in the highlands of wollo, north east ethiopia. | the prevalence and serotypic diversity of mannheimia [pasteurella] haemolytica and pasteurella trehalosi from nasal swabs, sera and abattoir specimens from sheep in the highlands of wollo, north east ethiopia was investigated. prevalence rates of 83% and 75% of these microorganisms were found in the serum samples and nasal swabs, respectively, from apparently healthy sheep. in a local abattoir, 205 lungs were investigated, 34% of which showed pneumonia, from which samples were collected from 51 ... | 2003 | 12625399 |
| novel protease produced by a pasteurella trehalosi serotype 10 isolate from a pneumonic bighorn sheep: characteristics and potential relevance to protection. | a strain of pasteurella trehalosi serotype 10, e(co)-100, isolated from a bighorn sheep that had succumbed to pneumonic pasteurellosis during an epizootic, was compared to well-characterized strains of p. trehalosi serotype 10 and mannheimia haemolytica serotype 1. the gene for leukotoxin a (lkta) from e(co)-100 was sequenced and found to be identical on an amino acid basis to a published sequence for lkta from p. trehalosi serotype 10. however, the toxic activity in culture supernatant measured ... | 2003 | 12637002 |
| sequence diversity and molecular evolution of the heat-modifiable outer membrane protein gene (ompa) of mannheimia(pasteurella) haemolytica, mannheimia glucosida, and pasteurella trehalosi. | the ompa (or heat-modifiable) protein is a major structural component of the outer membranes of gram-negative bacteria. the protein contains eight membrane-traversing beta-strands and four surface-exposed loops. the genetic diversity and molecular evolution of ompa were investigated in 31 mannheimia (pasteurella) haemolytica, 6 mannheimia glucosida, and 4 pasteurella trehalosi strains by comparative nucleotide sequence analysis. the ompa proteins of m. haemolytica and m. glucosida contain four h ... | 2004 | 15317779 |
| comparative phylogenies of the housekeeping genes atpd, infb and rpob and the 16s rrna gene within the pasteurellaceae. | phylogenies of housekeeping gene and 16s rrna gene sequences were compared to improve the classification of the bacterial family pasteurellaceae and knowledge of the evolutionary relationships of its members. deduced partial protein sequences of the housekeeping genes atpd, infb and rpob were compared in 28, 36 and 28 representative taxa of the pasteurellaceae, respectively. the monophyly of representatives of the genus gallibacterium was recognized by analysis of all housekeeping genes, while m ... | 2004 | 15388716 |
| serological and molecular comparison of mannheimia haemolytica and pasteurella trehalosi strains isolated from wild and domestic ruminants in the french alps. | over a period of 17 years, 84 bacterial isolates identified as mannheimia haemolytica or m. glucosida, and 52 isolates identified as pasteurella trehalosi were detected in the lungs of domestic and wild ruminants in the french alps. the isolates were serotyped according to their surface capsular antigens, and those sharing common antigens were further characterized by pulsed field gel electrophoresis. the results showed that the bacterial isolates included in the study clustered according to the ... | 2006 | 16624723 |
| complete nucleotide sequence of a p2 family lysogenic bacteriophage, varphimhaa1-phl101, from mannheimia haemolytica serotype a1. | the 34,525 nucleotide sequence of a double-stranded dna bacteriophage (phimhaa1-phl101) from mannheimia haemolytica serotype a1 has been determined. the phage encodes 50 open reading frames. twenty-three of the proteins are similar to proteins of the p2 family of phages. other protein sequences are most similar to possible prophage sequences from the draft genome of histophilus somni 2336. fourteen open reading frames encode proteins with no known homolog. the p2 orthologues are collinear in phi ... | 2006 | 16631219 |
| phylogenetic diversity of pasteurellaceae and horizontal gene transfer of leukotoxin in wild and domestic sheep. | wild and domestic animal populations are known to be sources and reservoirs of emerging diseases. there is also a growing recognition that horizontal genetic transfer (hgt) plays an important role in bacterial pathogenesis. we used molecular phylogenetic methods to assess diversity and cross-transmission rates of pasteurellaceae bacteria in populations of bighorn sheep, dall's sheep, domestic sheep and domestic goats. members of the pasteurellaceae cause an array of deadly illnesses including ba ... | 2007 | 16635591 |
| plasmid-mediated florfenicol resistance in pasteurella trehalosi. | a florfenicol-resistant pasteurella trehalosi isolate from a calf was investigated for the presence and the location of the gene flor. | 2006 | 16670108 |
| diversity of temperate bacteriophages induced in bovine and ovine mannheimia haemolytica isolates and identification of a new p2-like phage. | the diversity of temperate bacteriophages was examined in 32 mannheimia haemolytica, six mannheimia glucosida and four pasteurella trehalosi isolates. phage particles were induced and identified by electron microscopy in 24 (75%) m. haemolytica isolates, but in only one (17%) m. glucosida and one (25%) p. trehalosi isolate. the m. haemolytica phages were relatively diverse as seven siphoviridae, 15 myoviridae and two podoviridae-like phages were identified; the myoviridae-type phages also exhibi ... | 2006 | 16842340 |
| reclassification of [pasteurella] trehalosi as bibersteinia trehalosi gen. nov., comb. nov. | [pasteurella] trehalosi is an important pathogen of sheep, being primarily associated with serious systemic infections in lambs but also having an association with pneumonia. the aim of the present investigation was to characterize a broad collection of strains tentatively identified as [p.] trehalosi in order to reclassify and rename this taxon to support improvements in our understanding of the pathogenesis and epidemiology of this important organism. the type strain for [p.] trehalosi, strain ... | 2007 | 17392184 |
| effect of vaccinating lambs against pneumonic pasteurellosis under new zealand field conditions on their weight gain and pneumonic lung lesions at slaughter. | in a field trial, 9174 lambs from seven commercial sheep flocks with a history of subclinical pneumonia were either vaccinated with ovipast plus (intervet) or given a placebo by systematic random allocation; they were vaccinated twice at an interval of four to six weeks and grazed on pasture in the same paddocks. they were weighed at the first vaccination, 11 and 23 weeks later, and one to three days before they were slaughtered. the extent of the pneumonic lesions in their lungs was scored visu ... | 2008 | 18178931 |
| serotypes a1 and a2 of mannheimia haemolytica are susceptible to genotypic, capsular and phenotypic variations in contrast to t3 and t4 serotypes of bibersteinia (pasteurella) trehalosi. | mannheimia haemolytica and bibersteinia (pasteurella) trehalosi are the most common bacterial isolates that cause pulmonary diseases in ruminants worldwide. the disease is determined by specific serotypes found in cattle and small ruminants. the molecular epidemiology of strains involved in disease is important in the control of outbreaks as well as in the preparation of vaccines. this study aimed to detect the instability and variations of bacterial strains that may affect the analysis of epide ... | 2008 | 18179587 |
| microorganisms associated with a pneumonic epizootic in rocky mountain bighorn sheep (ovis canadensis canadensis). | a comprehensive study of a pneumonic epizootic was initiated when the first signs of disease were noted in a metapopulation of bighorn sheep inhabiting hells canyon, bordering idaho, oregon, and washington. a total of 92 bighorn sheep were tested for etiologic agents during the following 6-mo study period. the study population included bighorn sheep believed to be the subpopulation in which disease was first noted, and these sheep were translocated to a holding facility in an effort to contain t ... | 2007 | 18229860 |
| [fatty acid composition of cells and lipopolysaccliarides of mannheimia haemolytica, mannheimia glucosida and bibersteinia trehalosi strains]. | the mannheimia haemolytica, mannheimia glucosida and bibersteinia trehalosi strains and the similar fatty acid composition of cells with domination of c(16:1) and c(16:0), which were in almost equal quantities, c(14:0 and c(18:1) + c(18:2). the fatty acid composition of lipopolysaccharides (lps) of the studied bacteria had no essential differences too. it was mainly represented by c(14:0) and 3-oh-c(14:0) which consisted of more than 80% of all lps fatty acids. c(12:0), c(16:1) and c(16:0) were ... | 2008 | 19351048 |
| variation in pasteurella (bibersteinia) and mannheimia spp. following transport and antibiotic treatment in free-ranging and captive rocky mountain bighorn sheep (ovis canadensis canadensis). | morbidity and mortality associated with respiratory disease following capture and translocation of bighorn sheep (ovis canadensis canadensis) is a significant concern, particularly when establishing new or augmenting existing bighorn populations. administration of prophylactic antibiotics at the time of capture is often done to minimize the risk of respiratory disease, but the efficacy of this practice is unknown. the effects of oxytetracycline and florfenicol on the pasteurella (bibersteinia) a ... | 2009 | 19368250 |
| an ecologic study comparing distribution of pasteurella trehalosi and mannheimia haemolytica between sierra nevada bighorn sheep, white mountain bighorn sheep, and domestic sheep. | the prevalence and phenotypic variability of pasteurella and mannheimia isolates from sierra nevada bighorn sheep (ovis canadensis sierrae), white mountain bighorn sheep (ovis canadensis nelsoni), and domestic sheep (ovis aries) from california, usa, were compared. the white mountain bighorn sheep population had a recent history of pneumonia-associated mortality, whereas the sierra nevada bighorn sheep population had no recent history of pneumonia-associated mortality. the domestic sheep flocks ... | 2009 | 19901369 |
| bibersteinia trehalosi inhibits the growth of mannheimia haemolytica by a proximity-dependent mechanism. | mannheimia (pasteurella) haemolytica is the only pathogen that consistently causes severe bronchopneumonia and rapid death of bighorn sheep (bhs; ovis canadensis) under experimental conditions. paradoxically, bibersteinia (pasteurella) trehalosi and pasteurella multocida have been isolated from bhs pneumonic lungs much more frequently than m. haemolytica. these observations suggest that there may be an interaction between these bacteria, and we hypothesized that b. trehalosi overgrows or otherwi ... | 2010 | 20038698 |
| bibersteinia trehalosi necrotising hepatitis associated with sudden death in an adult cow. | | 2010 | 20643888 |
| evidence for a common gene pool and frequent recombinational exchange of the tbpba operon in mannheimia haemolytica, mannheimia glucosida and bibersteinia trehalosi. | the tbpba operon was sequenced in 42 representative isolates of mannheimia haemolytica (32), mannheimia glucosida (6) and bibersteinia trehalosi (4). a total of 27 tbpb and 20 tbpa alleles were identified whilst the tbpba operon was represented by 28 unique alleles that could be assigned to seven classes. there were 1566 (34.8% variation) polymorphic nucleotide sites and 482 (32.1% variation) variable inferred amino acid positions among the 42 tbpba sequences. the tbpba operons of serotype a2 m. ... | 2010 | 20884693 |
| a bighorn sheep die-off in southern colorado involving a pasteurellaceae strain that may have originated from syntopic cattle. | we investigated a pasteurellosis epizootic in free-ranging bighorn sheep (ovis canadensis) wherein a pasteurellaceae strain carried by syntopic cattle (bos taurus) under severe winter conditions appeared to contribute to pneumonia in affected bighorns. twenty-one moribund or dead bighorn sheep were found on the "fossil ridge" herd's winter range, colorado, usa, between 13 december 2007 and 29 february 2008. eight carcasses examined showed gross or microscopic evidence of acute to subacute fibrin ... | 2010 | 20966277 |
| domestic sheep (ovis aries) pasteurellaceae isolates from diagnostic submissions to the caine veterinary teaching center (1990-2004). | a retrospective study of pasteurellaceae isolated from domestic sheep (ovis aries) was conducted. the aim was to identify pasteurellaceae present in animals that were clinically healthy and others with evidence of respiratory disease. the bacteria had been isolated from samples submitted to the university of idaho caine veterinary teaching center as part of disease diagnostic testing. the 844 isolates identified mainly three species of pasteurellaceae: mannheimia haemolytica, pasteurella multoci ... | 2011 | 21345625 |
| a multivalent mannheimia/bibersteinia vaccine protects bighorn sheep against mannheimia haemolytica challenge. | bighorn sheep (bhs) are more susceptible than domestic sheep (ds) to mannheimia haemolytica pneumonia. although both species carry m. haemolytica as a commensal bacterium in the nasopharynx, ds carry mostly leukotoxin (lkt)-positive strains while bhs carry lkt-negative strains. consequently, antibodies to surface antigens and lkt are present at much higher titers in ds, but lower in bhs. the objective of this study was to determine whether repeated immunization of bhs with multivalent mannheimia ... | 2011 | 21832104 |
| subcutaneous botryomycosis due to bibersteinia trehalosi in a texas longhorn steer. | a 3-year-old texas longhorn steer had a long history of progressive swelling of the soft tissues of the jaw and neck. at necropsy, multifocal to coalescing dermal and subcutaneous pyogranulomas were surrounded by fibrous tissue. microscopically, the pyogranulomas contained aggregates of gram-negative coccobacilli surrounded by splendore-hoeppli material and were separated by bands of fibrovascular tissue (botryomycosis). phylogenetic analysis of multilocus sequence-typing data revealed that the ... | 2011 | 22194356 |
| antimicrobial susceptibility patterns and sensitivity to tulathromycin in goat respiratory bacterial isolates. | bacterial pneumonia is a common and often life-threatening respiratory problem in both meat and dairy goats. options for approved antibiotic therapy in goats to combat these bacterial infections are severely limited and frequently drugs must be used in an extra-label manner. tulathromycin, a triamilide macrolide antimicrobial drug shown to be effective against swine and cattle respiratory bacterial agents, has been identified as a potentially useful drug in caprines. the present study was conduc ... | 2011 | 22078276 |
| comparative methylome analysis of the occasional ruminant respiratory pathogen bibersteinia trehalosi. | we examined and compared both the methylomes and the modification-related gene content of four sequenced strains of bibersteinia trehalosi isolated from the nasopharyngeal tracts of nebraska cattle with symptoms of bovine respiratory disease complex. the methylation patterns and the encoded dna methyltransferase (mtase) gene sets were different between each strain, with the only common pattern being that of dam (gatc). among the observed patterns were three novel motifs attributable to type i re ... | 2016 | 27556252 |
| frederiksenia canicola gen. nov., sp. nov. isolated from dogs and human dog-bite wounds. | polyphasic analysis was done on 24 strains of bisgaard taxon 16 from five european countries and mainly isolated from dogs and human dog-bite wounds. the isolates represented a phenotypically and genetically homogenous group within the family pasteurellaceae. their phenotypic profile was similar to members of the genus pasteurella. matrix-assisted laser desorption/ionization time-of-flight mass spectrometry clearly identified taxon 16 and separated it from all other genera of pasteurellaceae sho ... | 2014 | 24510449 |
| assessing timing and causes of neonatal lamb losses in a bighorn sheep ovis canadensis canadensis herd via use of vaginal implant transmitters. | we evaluated the use of vaginal implant transmitters (vits) as a means of detecting, capturing, and radio collaring rocky mountain bighorn sheep ( ovis canadensis canadensis) lambs to estimate survival and to facilitate carcass recovery to assess causes of mortality. we focused on one of several bighorn herds in colorado, usa, suffering from depressed recruitment that was not preceded by a classic all-age die-off. we captured, radio-collared, diagnosed pregnancy by ultrasound examination, and in ... | 2017 | 28192042 |
| disease complexity in a declining alaskan muskox (ovibos moschatus) population. | the muskox ( ovibos moschatus ) population inhabiting the eastern north slope (ens) of alaska, us declined dramatically during 1999-2006, whereas populations in western alaska (wa) were stable or increasing. to understand morbidity and mortality factors contributing to the decline, alaska department of fish and game conducted pathologic investigations of carcasses from 2005 until 2008. additionally, archived sera from both ens and wa muskoxen collected during 1984-92, before the documented begin ... | 2017 | 28099077 |
| mannheimia haemolytica and bibersteinia trehalosi serotypes isolated from merino breed lambs in extremadura (southwestern spain). | pneumonia caused by mannheimia haemolytica is an important disease in ruminants. because of its economic significance, several methods have been developed to study the pathogenicity and epidemiology of m. haemolytica. in this study, bacterial isolates of m. haemolytica and bibersteinia trehalosi identified from the lungs of sheep were serotyped by means of indirect haemagglutination. of the 598 lungs studied, 34 isolates were identified and serotyped. in decreasing order, m. haemolytica serotype ... | 2016 | 27784951 |
| how respiratory pathogens contribute to lamb mortality in a poorly performing bighorn sheep ( ovis canadensis ) herd. | we evaluated bighorn sheep ( ovis canadensis ) ewes and their lambs in captivity to examine the sources and roles of respiratory pathogens causing lamb mortality in a poorly performing herd. after seven consecutive years of observed december recruitments of <10%, 13 adult female bighorn sheep from the remnant gribbles park herd in colorado, us were captured and transported to the thorne-williams wildlife research center in wyoming in march 2013. ewes were sampled repeatedly over 16 mo. in april ... | 2017 | 27690193 |
| genome sequence of bibersteinia trehalosi strain y31 isolated from the pneumonic lung of a bighorn sheep. | here, we report the genome sequence for bibersteinia trehalosi strain y31, isolated from the lungs of a bighorn sheep (ovis canadensis) that had succumbed to pneumonia, which exhibits proximity-dependent inhibition (pdi) of mannheimia haemolytica the sequence will be used to understand the mechanism of pdi for these organisms. | 2016 | 27445392 |
| fusobacterium necrophorum in north american bighorn sheep ( ovis canadensis ) pneumonia. | fusobacterium necrophorum has been detected in pneumonic bighorn sheep (bhs; ovis canadensis ) lungs, in addition to the aerobic respiratory pathogens mannheimia haemolytica , bibersteinia trehalosi , pasteurella multocida , and mycoplasma ovipneumoniae . similar to m. haemolytica , f. necrophorum produces a leukotoxin. leukotoxin-induced lysis and degranulation of polymorphonuclear leukocytes (pmns) and macrophages are responsible for acute inflammation and lung tissue damage characteristic of ... | 2016 | 27224212 |
| northern ireland disease surveillance report, october to december 2015. | ·pneumonia and encephalitis due to histophilus somni in heifers ·pneumonia due to bibersteinia trehalosi in a cow ·fasciolosis in ewes and lambs ·dosing gun injuries in lambs ·histomonosis in chickens these are among matters discussed in the northern ireland animal disease surveillance quarterly report for october to december 2015. | 2016 | 26868239 |
| a semi-multifunctional sialyltransferase from bibersteinia trehalosi and its comparison to the pasteurella multocida st1 mutants. | sialic acids are well known for their crucial roles in many physiological and pathological processes. improvement in the efficacy of protein drugs, an increase in the anti-inflammatory activity of intravenous immunoglobulin, preparation of infant milk and the diagnosis of diseases are examples of why there is a need for efficient in vitro sialylation. sialyltransferases are crucial enzymes for the synthesis of sialo-oligosaccharides. here, we introduce a new α2,3-sialyltransferase from bacteria ... | 2015 | 26477829 |
| ursidibacter maritimus gen. nov., sp. nov. and ursidibacter arcticus sp. nov., two new members of the family pasteurellaceae isolated from the oral cavity of bears. | thirty-three suspected strains of the family pasteurellaceae isolated from the oral cavity of polar and brown bears were characterized by genotypic and phenotypic tests. phylogenetic analysis of partial 16s rrna gene and rpob sequences showed that the investigated isolates formed two closely related monophyletic groups, representing two novel species of a new genus. based on 16s rrna gene sequence comparison bibersteinia trehalosi was the closest related species with a validly published name, wi ... | 2015 | 26220443 |
| intercurrent tickborne fever infection and bibersteinia trehalosi septicaemia in a five-week-old lamb. | | 2015 | 26139677 |
| bibersteinia trehalosi infection in cattle and sheep. | | 2015 | 25698822 |
| serum concentrations of haptoglobin and haptoglobin-matrix metalloproteinase 9 (hp-mmp 9) complexes of bovine calves in a bacterial respiratory challenge model. | serum haptoglobin (hp) and haptoglobin matrix metalloproteinase 9 complexes (hp-mmp 9) have been identified as biomarkers with diagnostic potential in cattle with conditions resulting in an acute inflammatory response. the purpose of this study was to evaluate potential diagnostic applications of serum hp and hp-mmp 9 concentrations in calves with brd and establish a timeline for their detection in calves experimentally challenged with bibersteinia trehalosi and mannheimia haemolytica. thirty-fi ... | 2014 | 25480520 |
| epizootic pneumonia of bighorn sheep following experimental exposure to mycoplasma ovipneumoniae. | bronchopneumonia is a population limiting disease of bighorn sheep (ovis canadensis). the cause of this disease has been a subject of debate. leukotoxin expressing mannheimia haemolytica and bibersteinia trehalosi produce acute pneumonia after experimental challenge but are infrequently isolated from animals in natural outbreaks. mycoplasma ovipneumoniae, epidemiologically implicated in naturally occurring outbreaks, has received little experimental evaluation as a primary agent of bighorn sheep ... | 2014 | 25302992 |
| growth of mannheimia haemolytica: inhibitory agents and putative mechanism of inhibition. | leukotoxin-producing mannheimia haemolytica consistently causes fatal pneumonia in bighorn sheep (bhs) under experimental conditions. surprisingly, by culture methods, it has been isolated from pneumonic bhs lungs less frequently than other bacteria. however, in one study pcr assays detected m. haemolytica from over 70% of the pneumonic lung samples that were negative for this organism by culture, suggesting that the growth of m. haemolytica is inhibited by other bacteria. previously, we have sh ... | 2014 | 25246231 |
| efficacy of a multivalent modified-live virus vaccine containing a mannheimia haemolytica toxoid in calves challenge exposed with bibersteinia trehalosi. | to determine the efficacy of a multivalent modified-live virus (mlv) vaccine containing a mannheimia haemolytica toxoid to reduce pneumonia and mortality rate when administered to calves challenge exposed with virulent bibersteinia trehalosi. animals-74 holstein calves. | 2014 | 25061709 |
| acute die-off of chamois (rupicapra rupicapra) in the eastern austrian alps due to bacterial bronchopneumonia with pasteurellaceae. | monitoring circulating pathogens in wildlife populations is important in evaluating causes and sources of disease as well as understanding transmission between wild and domestic animals. in spring 2010, a sudden die-off in a chamois (rupicapra rupicapra) population sharing habitat with livestock occurred in northeastern austria. nineteen animals were submitted for examination. necropsy and pathohistologic and bacteriologic results yielded lesions associated with pasteurellaceae species. addition ... | 2014 | 24807183 |
| randomized clinical trial to evaluate the pathogenicity of bibersteinia trehalosi in respiratory disease among calves. | bibersteinia trehalosi causes respiratory disease in ruminants particularly in wild and domestic sheep. recently, there has been an increased number of b. trehalosi isolates obtained from diagnostic samples from bovine respiratory disease cases. this study evaluated the role of b. trehalosi in bovine respiratory disease using an intra-tracheal inoculation model in calves. thirty six cross bred 2-3 month old dairy calves were inoculated intra-tracheally with either leukotoxin negative b. trehalos ... | 2014 | 24745347 |
| bighorn sheep × domestic sheep hybrids survive mannheimia haemolytica challenge in the absence of vaccination. | bighorn sheep (bhs, ovis canadensis) are much more susceptible than domestic sheep (ds, ovis aries) to pneumonia caused by leukotoxin (lkt)-producing members of the family pasteurellaceae, particularly mannheimia haemolytica and bibersteinia trehalosi. leukotoxin is widely accepted as the critical virulence factor of these bacteria since lkt-negative mutants do not cause death of bhs. typically, ds carry lkt-positive m. haemolytica and/or b. trehalosi as commensal bacteria in their nasopharynx. ... | 2014 | 24629771 |
| complete closed genome sequences of three bibersteinia trehalosi nasopharyngeal isolates from cattle with shipping fever. | bibersteinia trehalosi is a respiratory pathogen affecting cattle and related ruminants worldwide. b. trehalosi is closely related to mannheimia haemolytica and is often associated with bovine respiratory disease complex (brdc), a polymicrobial multifactorial disease. we present three complete closed genome sequences of this species generated using an automated assembly pipeline. | 2014 | 24526647 |
| pcr assay detects mannheimia haemolytica in culture-negative pneumonic lung tissues of bighorn sheep (ovis canadensis) from outbreaks in the western usa, 2009-2010. | mannheimia haemolytica consistently causes severe bronchopneumonia and rapid death of bighorn sheep (ovis canadensis) under experimental conditions. however, bibersteinia trehalosi and pasteurella multocida have been isolated from pneumonic bighorn lung tissues more frequently than m. haemolytica by culture-based methods. we hypothesized that assays more sensitive than culture would detect m. haemolytica in pneumonic lung tissues more accurately. therefore, our first objective was to develop a p ... | 2014 | 24171569 |
| phylogenetic and epidemiologic relationships among pasteurellaceae from colorado bighorn sheep herds. | we used 16s rrna sequencing and leukotoxin gene (lkta) screening via pcr assay to clarify phylogenetic and epidemiologic relationships among pasteurellaceae isolated from bighorn sheep (ovis canadensis). only six of 21 bighorn isolates identified as "mannheimia haemolytica" in original laboratory reports appeared to be isolates of m. haemolytica sensu stricto based on 16s rrna sequence comparisons; the remainder grouped with m. glucosida (n=8) or m. ruminalis (n=7). similarly, 16s rrna sequence ... | 2013 | 23778616 |
| diversity of bacterial species in the nasal cavity of sheep in the highlands of ethiopia and first report of histophilus somni in the country. | a study was conducted to isolate bacterial species/pathogens from the nasal cavity of apparently healthy and pneumonic sheep. nasal swabs were collected aseptically, transported in tryptose soya broth and incubated for 24 h. then, each swab was streaked onto chocolate and blood agar for culture. bacterial species were identified following standard bacteriological procedures. accordingly, a total of 1,556 bacteria were isolated from 960 nasal swabs collected from three different highland areas of ... | 2013 | 23328945 |
| role of bibersteinia trehalosi, respiratory syncytial virus, and parainfluenza-3 virus in bighorn sheep pneumonia. | pneumonic bighorn sheep (bhs) have been found to be culture- and/or sero-positive for bibersteinia trehalosi, respiratory syncytial virus (rsv), and parainfluenza-3 virus (pi-3). the objective of this study was to determine whether these pathogens can cause fatal pneumonia in bhs. in the first study, two groups of four bhs each were intra-tracheally administered with leukotoxin-positive (group i) or leukotoxin-negative (group ii) b. trehalosi. all four animals in group i developed severe pneumon ... | 2013 | 23025980 |
| proximity-dependent inhibition of growth of mannheimia haemolytica by pasteurella multocida. | mannheimia haemolytica, pasteurella multocida, and bibersteinia trehalosi have been identified in the lungs of pneumonic bighorn sheep (bhs; ovis canadensis). of these pathogens, m. haemolytica has been shown to consistently cause fatal pneumonia in bhs under experimental conditions. however, m. haemolytica has been isolated by culture less frequently than the other bacteria. we hypothesized that the growth of m. haemolytica is inhibited by other bacteria in the lungs of bhs. the objective of th ... | 2012 | 22798357 |
| pasteurellaceae isolated from bighorn sheep (ovis canadensis) from idaho, oregon, and wyoming. | to elucidate the species and biovariants of pasteurellaceae isolated from clinically normal bighorn sheep (ovis canadensis) or bighorn sheep with evidence of respiratory disease. | 2012 | 22738054 |
| causes of pneumonia epizootics among bighorn sheep, western united states, 2008-2010. | epizootic pneumonia of bighorn sheep is a devastating disease of uncertain etiology. to help clarify the etiology, we used culture and culture-independent methods to compare the prevalence of the bacterial respiratory pathogens mannheimia haemolytica, bibersteinia trehalosi, pasteurella multocida, and mycoplasma ovipneumoniae in lung tissue from 44 bighorn sheep from herds affected by 8 outbreaks in the western united states. m. ovipneumoniae, the only agent detected at significantly higher prev ... | 2012 | 22377321 |