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inhibition of initial adhesion of uropathogenic enterococcus faecalis by biosurfactants from lactobacillus isolates.in this study, 15 lactobacillus isolates were found to produce biosurfactants in the mid-exponential and stationary growth phases. the stationary-phase biosurfactants from lactobacillus casei subsp. rhamnosus 36 and atcc 7469, lactobacillus fermentum b54, and lactobacillus acidophilus rc14 were investigated further to determine their capacity to inhibit the initial adhesion of uropathogenic enterococcus faecalis 1131 to glass in a parallel-plate flow chamber. the initial deposition rate of e. fa ...19968787394
effect of lactobacillus rhamnosus strain gg (atcc 53103) on the growth of streptococcus sobrinus in vitro.lactobacillus gg, a recently characterized l. rhamnosus gg strain (atcc 53103), has been shown to exert inhibitory activity against a variety of bacterial species, including streptococci. we isolated and studied the effect of the inhibitory substance of lactobacillus gg on some oral streptococci. the inhibitory activity of the isolated substance was weak, but some growth inhibition was observed in streptococcus sobrinus pretreated with the substance in comparison with untreated controls. zones o ...19957552958
lactobacillus rhamnosus septicemia in patients with prolonged aplasia receiving ceftazidime-vancomycin. 19912009881
lipoquinones of some spore-forming rods, lactic-acid bacteria and actinomycetes.the respiratory quinones of 73 strains of gram-positive bacteria including spore-forming rods, lactic-acid bacteria and actinomyctes were examined. menaquinones with seven isoprenoid units (mk-7) were the main quinone type found in representatives of the genus bacillus and in sporolactobacillus inulinus. however, a strain of b. thuringiensis produced mk-8 in addition to mk-7, and strains of b. lentus and b. pantothenticus appeared to produce mk-9 and mk-8, respectively, with no mk-7. in the clos ...1979119033
the effect of lactobacillus gg on the initiation and promotion of dmh-induced intestinal tumors in the rat.male fischer 344 rats were fed a 20% or a 5% corn oil diet and were injected subcutaneously with dimethylhydrazine (dmh) weekly for 16 weeks. in addition, an approximately equal number of animals challenged with dmh were fed daily, until the end of the study, 2 x 10(10) lactobacillus casei subsp. rhamnosus strain gg starting three weeks before dmh administration or after the ninth weekly injection. the feeding of the lactobacillus gg before and during carcinogen treatment resulted in a significa ...19968710689
absence of cholic acid 7 alpha-dehydroxylase activity in the strains of lactobacillus and bifidobacterium.to investigate the presence of 7 alpha-dehydroxylase activity on bile acids in the bacterial strains of fermented milk products, 46 strains of lactobacillus casei, lactobacillus paracasei, lactobacillus rhamnosus, lactobacillus acidophilus, lactobacillus gasseri, bifidobacterium adolescentis, bifidobacterium bifidum, bifidobacterium breve, bifidobacterium infantis, bifidobacterium longum, lactococcus lactis spp. lactis, and streptococcus salivarius spp. thermophilus were tested for their ability ...19947814703
effect of acetate on sorbitol fermentation by oral lactobacilli.the rate of acid production and end-products from sorbitol were measured under anaerobic conditions in washed-cell suspensions of oral strains of lactobacillus casei subsp. casei and lactobacillus casei subsp. rhamnosus. the enzymatic activities were assayed in cell extracts of these strains. the cells fermented sorbitol to lactate, formate, ethanol and acetate under anaerobic conditions. exposure of the cells to air (oxygen) led to inactivation of pyruvate formate-lyase and inhibition of anaero ...19958602342
the aggregation of human platelets by lactobacillus species.the ability to aggregate human platelets was examined for five lactobacillus rhamnosus strains and five lactobacillus paracasei subsp. paracasei strains isolated from patients with infective endocarditis (ie), 25 laboratory isolates from the same two species, and 14 strains from five other oral species, namely lactobacillus acidophilus, lactobacillus fermentum, lactobacillus oris, lactobacillus plantarum and lactobacillus salivarius. amongst the l. rhamnosus strains, platelets were aggregated by ...19938126421
enzyme production by lactobacilli and the potential link with infective endocarditis.fifty-six strains of lactobacilli were examined for the production of glycosidases and proteases (arylamidases) that could be associated with the ability to grow in vivo and/or be a factor in the pathogenesis of endocarditis. the strains were from seven species, with an emphasis on lactobacillus rhamnosus and lact. paracasei subsp. paracasei, both of which have been associated with endocarditis and provided 12 of the 13 strains isolated from cases of the disease. other species were lact. acidoph ...19957698950
lactobacillus rhamnosus infection in a child following bone marrow transplant.we report a case where lactobacillus rhamnosus was isolated from pericardial effusion and blood in a child following a bone marrow transplant for aplastic anaemia. a resume of cases in which this organism has been implicated as a pathogen is also presented.19968708379
intra-species characterization of clinical isolates and biotechnologically used strains of lactobacillus rhamnosus by analysis of the total soluble cytoplasmatic proteins with silver staining.lactobacillus rhamnosus is often used in milk products because of its technological properties, however it is supposed to be related to human infections as well. therefore, 23 l. rhamnosus strains, including the type strain, were investigated. nine strains resulted from biotechnological sources, 14 isolates had a clinical background, 13 came from a swedish culture collection. biochemical and physiological properties were proved by classical tests. analysis of the total soluble cytoplasmatic prot ...19957654512
vancomycin resistance factor of lactobacillus rhamnosus gg in relation to enterococcal vancomycin resistance (van) genes.lactobacillus rhamnosus gg (atcc 53103) is a probiotic strain used in fermented dairy products in many countries and is also used as a food supplement in the form of freeze-dried powder. the relationship of the vancomycin resistance factor in l. rhamnosus gg and the vancomycin resistance (van) genes of enterococcus faecalis and e. faecium were studied using polymerase chain reaction (pcr), southern hybridization and conjugation methods. our results show that the vancomycin resistance determinant ...19989706787
effect of growth conditions on production of rhamnose-containing cell wall and capsular polysaccharides by strains of lactobacillus casei subsp. rhamnosus.strains of lactobacillus casei subsp. rhamnosus possessing two cell wall polysaccharides, a hexosamine-containing h-polysaccharide and a rhamnose-containing r-polysaccharide, were examined for the effect of growth conditions on the production of these two components. in strain nctc 6375, r- and h-polysaccharides accounted for an estimated 44 and 20%, respectively, of the cell wall for organisms grown in batch culture with glucose as the carbohydrate source. growth on fructose-containing media re ...19836401290
nucleic acids of species of lactobacillus.nucleic acid composition of 17 cultures representing the type or neotype strains of 15 named lactobacillus species was studied. nucleic acid characterization of these isolates was accompanied by a comparative study of conventional phenotypic reactions. the overall guanine plus cytosine mean deoxyribonucleic acid base composition ranged from 33 to 50% and genome sizes varied between 700 and 1500 x 10(6) daltons. sporolactobacillus inulinus contained 2500 x 10(6) daltons of chromosomal deoxyribonu ...19853939582
the colonization of a simulator of the human intestinal microbial ecosystem by a probiotic strain fed on a fermented oat bran product: effects on the gastrointestinal microbiota.the effects of lactobacillus-gg-fermented oat bran product on the microbiota and its metabolic activity in the human gut were investigated, using a simulator of the human intestinal microbial ecosystem (shime), by analysing the bacterial population, shortchain fatty acids and gas production. in addition, the effects of fermented oat bran supernatant and supernatant samples from reactors 4, 5 and 6 (large intestine) on the growth of escherichia coli ihe 13047, enterococcus faecalis vtt e-93203, l ...19989763692
antibodies in normal and diseased pulps reactive with microorganisms isolated from deep caries.immunoglobulin molecules in the supernatant fluids (sf) from pulpal explant cultures have been observed to react with microorganisms implicated in infections of root canals. in this study, the reactivity of immunoglobulin molecules in the sf from normal and irreversible pulpitis pulps to six strains of predominant microorganisms isolated from the immediate layer of carious lesions above the pulps used for explant cultures was investigated using an enzyme-linked immunosorbent assay. two atcc stra ...19921402561
capd peritonitis caused by lactobacillus rhamnosus. 19921363113
[effect of glucose and lactose on the utilization of citrate by lactobacillus casei subsp. rhamnosus atcc 7469].the utilization of citrate by lactobacillus casei subsp. rhamnosus atcc 7469 in a complex medium containing glucose, lactose or citrate was investigated, as an approach to the question of the transport of this acid and the possible relationship with the production of flavour compounds (diacetyl and acetoin). this lactobacillus uses citrate as an energy source in the absence of carbohydrates. external ph and growth increases when citrate is added to complex medium. the presence of citrate does no ...19921302866
effect of nutrient composition on the in vitro growth of urogenital lactobacilli and uropathogens.previous clinical studies have shown that nutrients and probiotic agents can alter the composition of the vaginal flora. the present in vitro study has shown that uropathogens have a growth advantage over lactobacilli, but potentially there are natural substances that could be applied vaginally to stimulate lactobacilli growth to the detriment of the pathogens. when chemically defined medium representative of vaginal fluid at ph 5.5 was supplemented with skim milk, it acted as a better substrate ...19989851026
lactobacillus rhamnosus gg (atcc 53103) and platelet aggregation in vitro.lactobacillus rhamnosus gg is an experimentally and clinically well documented probiotic used in different dairy products. the present study aimed to investigate the safety aspects of lactobacillus rhamnosus gg, particularly with respect to platelet aggregation, the initiating event in thrombosis. platelet rich plasma was separated from the blood of healthy volunteers, and the effects of lactobacillus rhamnosus gg (atcc 53103), lactobacillus rhamnosus (atcc 7469) and enterococcus faecium t2l6 in ...19979237125
purification and characterization of an aminopeptidase from lactobacillus casei subsp. rhamnosus s93.an aminopeptidase of broad specificity was extracted by cell lysis of a selected strain of lactobacillus casei subsp. rhamnosus during the late exponential phase. the enzyme was purified 195-fold from crude extract by using an f.p.l.c. system. native and sds/page of the purified enzyme showed a single protein band of 89 kda. the maximum aminopeptidase activity was observed at ph 7.0 and 39 degrees c. the enzyme hydrolysed a range of nitroanilide-substituted amino acids, as well as dipeptides, an ...19948192866
effect of age, amount of inoculum and inoculation medium composition on lactic acid production from glucose by lactobacillus casei subsp. rhamnosus.effect of composition of the medium used for the inoculum cultivation, of the age and amount of the inoculum was investigated using a 3-l glass fermentor with a working volume of 1 l. the highest productivity of the culture was obtained when using a 20% (v/v) 1-d inoculum grown in the mrs medium. yields of lactic acid were 88-97%, while the l(+)-isomer represented about 80% of the total product.19911841859
optimization of l-(+)-lactic acid production by ring and disc plastic composite supports through repeated-batch biofilm fermentation.four customized bioreactors, three with plastic composite supports (pcs) and one with suspended cells (control), were operated as repeated-batch fermentors for 66 days at ph 5 and 37 degrees c. the working volume of each customized reactor was 600 ml, and each reactor's medium was changed every 2 to 5 days for 17 batches. the performance of pcs bioreactors in long-term biofilm repeated-batch fermentation was compared with that of suspended-cell bioreactors in this research. pcs could stimulate b ...19979212403
ingredient selection for plastic composite supports for l-(+)-lactic acid biofilm fermentation by lactobacillus casei subsp. rhamnosus.plastic composite supports containing 50% agricultural products (oat hulls, soybean hulls, yeast extract, soybean flour, dried bovine erythrocytes, bovine albumin, and/or mineral salts) and 50% (wt/wt) polypropylene were produced by high-temperature twin-screw extrusion. the research employed two half sets of a five-factorial fractional design (2(5 - 1)) to evaluate the effects of different agricultural components on the properties of the plastic composite supports and to select the best plastic ...19979212402
use of virginiamycin to control the growth of lactic acid bacteria during alcohol fermentation.the antibiotic virginiamycin was investigated for its effects on growth and lactic acid production by seven strains of lactobacilli during the alcoholic fermentation of wheat mash by yeast. the lowest concentration of virginiamycin tested (0.5 mg lactrol kg-1 mash), was effective against most of the lactic acid bacteria under study, but lactobacillus plantarum was not significantly inhibited at this concentration. the use of virginiamycin prevented or reduced potential yield losses of up to 11% ...19979172435
development of oligonucleotide primers from the 16s-23s rrna intergenic sequences for identifying different dairy and probiotic lactic acid bacteria by pcr.spacer regions between the 16s and 23s rrna genes of different dairy and probiotic lactic acid bacteria were amplified by the polymerase chain reaction (pcr) with conserved primers and the nucleotide sequences of these spacer regions were determined. amplification/oligonucleotide primer pairs were designed for lactobacillus paracasei, lactobacillus rhamnosus, lactobacillus delbrueckii, lactobacillus acidophilus, lactobacillus helveticus and streptococcus thermophilus based on the differences in ...19979081225
reclassification of lactobacillus casei subsp. casei atcc 393 and lactobacillus rhamnosus atcc 15820 as lactobacillus zeae nom. rev., designation of atcc 334 as the neotype of l. casei subsp. casei, and rejection of the name lactobacillus paracasei.the type strain of lactobacillus casei subsp. casei (atcc 393) exhibits low levels of dna homology with other strains of l. casei subsp. casei (8 to 46%) and strains of lactobacillus paracasei (30 to 50%), but exhibits a level of dna similarity of 80% with lactobacillus rhamnosus atcc 15820, the original type strain of "lactobacterium zeae" kuznetsov 1959. strains atcc 393t (t = type strain) and atcc 15820t are members of one protein profile cluster that is separate from the other lactobacillus ...19968573516
comparative sequence analyses of the genes coding for 16s rrna of lactobacillus casei-related taxa.the primary structures of the 16s rrna genes of the type strains of lactobacillus casei and related taxa were determined by pcr dna-sequencing methods. the sequences of lactobacillus casei, lactobacillus zeae, lactobacillus paracasei, and lactobacillus rhamnosus were different. the knuc values ranged from 0.0040 to 0.0126. on the basis of the knuc values and the levels of dna-dna relatedness among the strains of these species, the l. casei-related taxa should be classified in the following three ...19978995801
a microbiology investigation on probiotic pharmaceutical products used for human health.many and different probiotic pharmaceutical products are presently commercialised in the world. on this regard, a microbiological investigation was carried out to screen the microorganisms incorporated into these products, commonly used for human health. after determination of the cell number and viability of bacteria, several experiments were performed in vitro in order to characterise the microorganisms and to evaluate their probiotic value. among all the strains identified, best results were ...19979265768
in vitro studies of porcine lactobacilli for possible probiotic use.fourteen strains of lactobacilli isolated from the gut of suckling pigs were identified and studied to determine their susceptibility to antimicrobial feed additives, acid tolerance, adherence to epithelial cells from the porcine intestine and antimicrobial activity. four strains were identified as lactobacillus casei subsp. casei, two strains as lactobacillus rhamnosus as well as lactobacillus reuteri and three strains as lactobacillus salivarius. the remaining strains could not be identified. ...19979451838
inhibition of purified enolases from oral bacteria by fluoride.enolase activity in strains of oral streptococci previously has been found to be inhibited by 50% (ki) by fluoride concentrations ranging from 50 to 300 microm or more in the presence of 0.5 to 1.0 mm inorganic phosphate ions. in this study, enolase was extracted and partly purified by a two-step process from five oral bacterial species and the effect of fluoride on the kinetics of enolase examined. the molecular weight of the putative enolase proteins was 46-48 kda. the vmax values ranged from ...19979227132
lactobacillus species associated with active caries lesions.lactobacilli that are most frequently associated with active caries lesions in dentine were isolated and identified as part of a research project which aims to determine the role of the predominant species of these organisms in the carious process. samples of carious dentine were collected from 12 patients with open caries lesions (group a) and stimulated saliva samples were collected from 12 patients with a dmft = 0 and confirmed presence of lactobacilli in the oral cavity (group b). after seri ...19989588995
peritonitis associated with vancomycin-resistant lactobacillus rhamnosus in a continuous ambulatory peritoneal dialysis patient: organism identification, antibiotic therapy, and case report.a case of lactobacillus rhamnosus-associated peritonitis in a patient undergoing continuous ambulatory peritoneal dialysis is reported. the patient was treated with vancomycin after isolation of glycopeptide-susceptible coagulase-negative staphylococci. after a skin rash developed, vancomycin was discontinued and replaced with teicoplanin. seven weeks after the glycopeptide therapy was discontinued, a lactobacillus strain was isolated in pure cultures. the isolate was identified first incorrectl ...19989620421
cloning and characterization of a prolinase gene (pepr) from lactobacillus rhamnosus.a peptidase gene expressing l-proline-beta-naphthylamide-hydrolyzing activity was cloned from a gene library of lactobacillus rhamnosus 1/6 isolated from cheese. peptidase-expressing activity was localized in a 1.5-kb saci fragment. a sequence analysis of the saci fragment revealed the presence of one complete open reading frame (orf1) that was 903 nucleotides long. the orf1-encoded 34.2-kda protein exhibited 68% identity with the pepr protein from lactobacillus helveticus. additional sequencing ...19989572959
ability of dairy strains of lactic acid bacteria to bind a common food carcinogen, aflatoxin b1.this study was conducted to examine the ability of selected dairy strains of lactic acid bacteria to remove aflatoxin b1 (afb1) from liquid media. both lactobacillus rhamnosus strain gg (lbgg) and l. rhamnosus strain lc-705 (lc705) can significantly (p > 0.05) remove afb1 when compared with that by other strains of either gram-positive or gram-negative bacteria. removal of afb1 by lbgg and lc705 was a rapid process with approximately 80% afb1 removed at 0 hr. removal of afb1 by these two strains ...19989651049
study of root caries in an artificial mouth.the object of this project was to develop a laboratory model system to study root and enamel caries. model plaque biofilms containing consortia of four (streptococcus mutans, streptococcus sobrinus, actinomyces naeslundii, and lactobacillus rhamnosus) or six (s mutans, s sobrinus, s mitis, a naeslundii, l rhamnosus, and veillonella dispar) species of caries pathogens were developed in a artificial mouth. caries lesions similar to those found in vivo were produced on enamel and dentine blocks and ...19989676475
physicochemical alterations enhance the ability of dairy strains of lactic acid bacteria to remove aflatoxin from contaminated media.lactobacillus rhamnosus gg and lactobacillus rhamnosus lc-705, previously shown to effectively bind to aflatoxin b1, were subjected to various chemical and physical treatments to examine the effects of these treatments on the binding affinity of these strains toward aflatoxin b1. treatment of bacterial pellets of both strains with hydrochloric acid significantly (p < 0.05) enhanced the binding ability to bind aflatoxin b1 was also observed when the bacterial pellets were subjected to heat treatm ...19989709211
analysis of ph-driven disruption of oral microbial communities in vitro.previously, a mixed culture chemostat system was used to demonstrate that the ph generated from carbohydrate metabolism, rather than carbohydrate availability per se, was responsible for the shifts observed in the oral microflora which are associated with high carbohydrate diets and the development of dental caries. the aim of this study was to determine more accurately the microbially generated ph at which such shifts occurred. nine oral bacteria were grown in three independent chemostats, and ...19989745120
persistence of colonization of human colonic mucosa by a probiotic strain, lactobacillus rhamnosus gg, after oral consumption.lactobacillus rhamnosus gg is one of the most thoroughly studied probiotic strains. its advantages in the treatment of gastrointestinal disorders are well documented. the aim of the present study was to demonstrate with colonic biopsies the attachment of strain gg to human intestinal mucosae and the persistence of the attachment after discontinuation of gg administration. a whey drink fermented with strain gg was fed to human volunteers for 12 days. fecal samples were collected before, during, a ...19999872808
recovery of lactobacillus rhamnosus gg from human colonic biopsies.the colonization of lactobacillus rhamnosus gg (atcc 53103, henceforth l.gg) in five human colonoscopy patients was studied. the test subjects consumed whey drink fermented with the bacterium for 12 d before the colonoscopy. the presence of l.gg was subsequently checked both in the faecal samples and in the colonic biopsies obtained from various locations in the large intestine. in all patients l.gg was the dominant faecal lactic acid bacterium as a result of the administration. in four patients ...19979172443
oat bran beta-gluco- and xylo-oligosaccharides as fermentative substrates for lactic acid bacteria.the influence of oat bran oligosaccharides on carbohydrate utilization and fermentation end-products was studied with reference to three different lactic acid bacteria (lab: lactobacillus rhamnosus, lactobacillus plantarum and lactococcus lactis). the main results were that all three lab utilized oat beta-gluco-oligosaccharides, while only l. plantarum utilized xylo-oligosaccharides. the main products of lab metabolism were lactic acid, acetic acid, formic acid and ethanol. the results indicated ...19989924948
strain-specific identification of probiotic lactobacillus rhamnosus with randomly amplified polymorphic dna-derived pcr primers.in the present work, strain-specific pcr primers for lactobacillus rhamnosus lc 1/3 are described. the randomly amplified polymorphic dna (rapd) technique was used to produce potential strain-specific markers. they were screened for specificity by hybridization with dna from 11 l. rhamnosus strains. a 613-bp rapd marker found to be strain-specific was sequenced, and a primer pair specific to l. rhamnosus lc 1/3 was constructed based on the sequence. the primer pair was tested with 11 lactobacill ...19989835567
the effect of lactose derivatives on intestinal lactic acid bacteria.nine strains of lactic acid bacteria were studied for growth and fermentation end products on lactulose, lactitol, and lactobionic acid. in addition, human fecal and biopsy isolates were screened for new potential by probiotic strains utilizing lactose derivatives, and one new isolate of lactobacillus rhamnosus was enriched. the utilization of lactose derivatives and the effect on the fermentation end products were dependent on strain. typical mixed-acid fermentations were observed with lb. rham ...199910068946
cell surface characteristics of lactobacillus casei subsp. casei, lactobacillus paracasei subsp. paracasei, and lactobacillus rhamnosus strains.hydrophilic and electrostatic cell surface properties of eight lactobacillus strains were characterized by using the microbial adhesion to solvents method and microelectrophoresis, respectively. all strains appeared relatively hydrophilic. the strong microbial adhesion to chloroform, an acidic solvent, in comparison with microbial adhesion to hexadecane, an apolar n-alkane, demonstrated the particularity of lactobacilli to have an important electron donor and basic character and consequently the ...19979143109
insertional inactivation of genes responsible for the d-alanylation of lipoteichoic acid in streptococcus gordonii dl1 (challis) affects intrageneric coaggregations.most human oral viridans streptococci participate in intrageneric coaggregations, the cell-to-cell adherence among genetically distinct streptococci. two genes relevant to these intrageneric coaggregations were identified by transposon tn916 mutagenesis of streptococcus gordonii dl1 (challis). a 626-bp sequence flanking the left end of the transposon was homologous to dlta and dltb of lactobacillus rhamnosus atcc 7469 (formerly called lactobacillus casei). a 60-kb probe based on this flanking se ...199910225909
effects of lactobacilli on yeast-catalyzed ethanol fermentations.normal-gravity (22 to 24 degrees plato) wheat mashes were inoculated with five industrially important strains of lactobacilli at approximately 10(5), approximately 10(6), approximately 10(7), approximately 10(8), and approximately 10(9) cfu/ml in order to study the effects of the lactobacilli on yeast growth and ethanol productivity. lactobacillus plantarum, lactobacillus paracasei, lactobacillus #3, lactobacillus rhamnosus, and lactobacillus fermentum were used. controls with yeast cells but no ...19979361399
regulated expression of the streptococcus mutans dlt genes correlates with intracellular polysaccharide accumulation.intracellular polysaccharides (ips) are glycogen-like storage polymers which contribute significantly to streptococcus mutans-induced cariogenesis. we previously identified and cloned a locus from the s. mutans chromosome which is required for the accumulation of ips. sequencing of this locus revealed at least four contiguous open reading frames, all of which are preceded by a common promoter region and are transcribed in the same direction. analysis of the amino acid sequence deduced from the f ...199910197997
probiotics inhibit enteropathogenic e. coli adherence in vitro by inducing intestinal mucin gene expression.probiotic agents, live microorganisms with beneficial effects for the host, may offer an alternative to conventional antimicrobials in the treatment and prevention of enteric infections. the probiotic agents lactobacillus plantarum 299v and lactobacillus rhamnosus gg quantitatively inhibited the adherence of an attaching and effacing pathogenic escherichia coli to ht-29 intestinal epithelial cells but did not inhibit adherence to nonintestinal hep-2 cells. ht-29 cells were grown under conditions ...199910198338
comparison of ribotyping, randomly amplified polymorphic dna analysis, and pulsed-field gel electrophoresis in typing of lactobacillus rhamnosus and l. casei strains.a total of 24 strains, biochemically identified as members of the lactobacillus casei group, were identified by pcr with species-specific primers. the same set of strains was typed by randomly amplified polymorphic dna (rapd) analysis, ribotyping, and pulsed-field gel electrophoresis (pfge) in order to compare the discriminatory power of the methods. species-specific primers for l. rhamnosus and l. casei identified the type strain l. rhamnosus atcc 7469 and the neotype strain l. casei atcc 334, ...199910473394
identification of lactobacillus isolates from the gastrointestinal tract, silage, and yoghurt by 16s-23s rrna gene intergenic spacer region sequence comparisons.lactobacillus isolates were identified by pcr amplification and sequencing of the region between the 16s and 23s rrna genes (spacer region). the sequences obtained from the isolates were compared to those of reference strains held in genbank. a similarity of 97.5% or greater was considered to provide identification. to check the reliability of the method, the v2-v3 region of the 16s rrna gene was amplified and sequenced in the case of isolates whose spacer region sequences were less than 99% sim ...199910473450
lactobacilli from human gastrointestinal mucosa are strong stimulators of il-12 production.interaction of macrophages with bacteria is a stimulus for production of cytokines such as il-10 and il-12. il-12 stimulates t cell and natural killer (nk) cell cytotoxicity and interferon-gamma (ifn-gamma) production. il-10 opposes the t cell-stimulating action of il-12, decreases the release of proinflammatory cytokines from macrophages, and stimulates b cells. we have studied the capacity of human intestinal isolates from the three lactobacillus species dominating on the human gastrointestina ...199910337019
differentiation of lactobacillus casei, lactobacillus paracasei and lactobacillus rhamnosus by polymerase chain reaction.lactobacillus casei, lact. paracasei and lact. rhamnosus form a closely related taxonomic group within the heterofermentative lactobacilli. these three species are difficult to differentiate using traditional fermentation profiles. we have developed polymerase chain reaction primers which are specific for each of these species based on differences in the v1 region of the 16s rrna gene. sixty-three lactobacillus isolates from cheese were identified using these primers. the 12 lact. rhamnosus and ...199910499296
efficiency of transcription from promoter sequence variants in lactobacillus is both strain and context dependent.the introduction of consensus -35 (ttgaca) and -10 (tataat) hexamers and a tg motif into the lactobacillus acidophilus atcc 4356 wild-type slpa promoter resulted in significant improvements (4.3-, 4.1-, and 10.7-fold, respectively) in transcriptional activity in lactobacillus fermentum br11. in contrast, the same changes resulted in decreased transcription in lactobacillus rhamnosus gg. the tg motif was shown to be important in the context of weak -35 and -10 hexamers (l. fermentum br11) or a co ...199910515955
antiproliferative effects of homogenates derived from five strains of candidate probiotic bacteria.unheated and heat-treated homogenates were separately prepared from candidate probiotic bacteria, including lactobacillus rhamnosus gg, bifidobacterium lactis, lactobacillus acidophilus, lactobacillus delbrueckii subsp. bulgaricus, and streptococcus thermophilus. we compared the phytohemagglutinin-induced proliferation of mononuclear cells in the presence of homogenates and in the presence of a control containing no homogenate by assessing thymidine incorporation in cell cultures. all homogenate ...199910543777
screening of probiotic activities of forty-seven strains of lactobacillus spp. by in vitro techniques and evaluation of the colonization ability of five selected strains in humans.the probiotic potential of 47 selected strains of lactobacillus spp. was investigated. the strains were examined for resistance to ph 2.5 and 0.3% oxgall, adhesion to caco-2 cells, and antimicrobial activities against enteric pathogenic bacteria in model systems. from the results obtained in vitro, five strains, lactobacillus rhamnosus 19070-2, l. reuteri dsm 12246, l. rhamnosus lgg, l. delbrueckii subsp. lactis chcc 2329, and l. casei subsp. alactus chcc 3137, were selected for in vivo studies. ...199910543808
[persistent lactobacillus casei subspecies rhamnosus bacteremia in a 14 year old girl with acute myeloid leukemia. a case report].the present clinical observation is related to a 14-year-old girl suffering from acute myeloid leukemia. the clinical course was complicated by episodes of severe enterocolitis, e. coli- septicemia, pancreatitis and pneumonia. in the course of continued cytostatic and antibiotic treatment a persistent asymptomatic lactobacillus casei subsp. rhamnosus-bacteremia became detectable by a total of 18 blood cultures. microbial cultures of the faeces revealed colony-forming unites of this germ in order ...199910407810
liver abscess due to a lactobacillus rhamnosus strain indistinguishable from l. rhamnosus strain gg. 199910452653
x-prolyl dipeptidyl aminopeptidase gene (pepx) is part of the glnra operon in lactobacillus rhamnosus.a peptidase gene expressing x-prolyl dipeptidyl aminopeptidase (pepx) activity was cloned from lactobacillus rhamnosus 1/6 by using the chromogenic substrate l-glycyl-l-prolyl-beta-naphthylamide for screening of a genomic library in escherichia coli. the nucleotide sequence of a 3.5-kb hindiii fragment expressing the peptidase activity revealed one complete open reading frame (orf) of 2,391 nucleotides. the 797-amino-acid protein encoded by this orf was shown to be 40, 39, and 36% identical with ...200010613874
development of multi-species consortia biofilms of oral bacteria as an enamel and root caries model system.the aim was to establish defined-species consortium plaque biofilms to investigate enamel and root caries in an artificial mouth. strains of the putative enamel and root caries pathogens, streptococcus mutans, strep. sobrinus, actinomyces naeslundii and lactobacillus rhamnosus, were screened in batch culture for potential cariogenic properties: a low terminal ph, ability to aggregate, and catabolic diversity. the strains selected were grown as monoculture biofilms and as consortium plaque biofil ...200010669090
identification of lactobacillus species of human origin by a commercial kit, api50chl.the efficacy of a biochemical kit, api50chl kit, for identification of intestinal and vaginal lactobacilli from humans was evaluated by comparing with the results of dna-dna hybridization assay. the results showed that in total only 52 of the 172 strains (30.2%) tested were identified correctly by the kit at species level. especially all strains of some species, such as lactobacillus gasseri and lactobacillus crispatus, were misidentified as lactobacillus acidophilus by the kit. however, the kit ...199910681709
inducible transport of citrate in lactobacillus rhamnosus atcc 7469.lactobacillus rhamnosus atcc 7469 exhibited diauxie when grown in a medium containing both glucose and citrate as energy source. glucose was used as the primary energy source during the glucose-citrate diauxie. uptake of citrate was carried out by an inducible citrate transport system. the induction of citrate uptake system was repressed in the presence of glucose. this repression was reversible and mediated by camp.19968896349
prophylactic lactobacillus gg reduces antibiotic-associated diarrhea in children with respiratory infections: a randomized study.antimicrobial treatment may disturb the colonization resistance of gastrointestinal microflora, which may induce clinical symptoms, most commonly diarrhea. the severity of antibiotic-associated diarrhea may range from a brief, self-limiting disease to devastating diarrhea with electrolyte disturbances, dehydration, crampy abdominal pain, pseudomembranous colitis, toxic megacolon, or even death. the incidence of diarrhea in children receiving a single antimicrobial treatment is unclear. in additi ...199910545590
effects of orally administered viable lactobacillus rhamnosus gg and propionibacterium freudenreichii subsp. shermanii js on mouse lymphocyte proliferation.immunomodulation by probiotics is a subject of growing interest, but the knowledge of dose response and time profile relationships is minimal. in this study we examined the effects of lactobacillus rhamnosus gg (lgg) and propionibacterium freudenreichii subsp. shermanii js (pjs) on the proliferative activity of murine lymphocytes ex vivo. dose dependency was assessed by treating animals perorally with a low or a high dose (i.e., 10(9) or 10(12) viable bacteria/kg of body weight) for 7 days. the ...199910548566
human ileostomy glycoproteins as a model for small intestinal mucus to investigate adhesion of probiotics.human ileostomy glycoproteins were used as a model for small intestinal mucus to investigate the adhesion of 12 lactobacillus strains, one lactococcus strain and one propionibacterium strain, both probiotic and dairy strains were tested. adhesive and non-adhesive escherichia coli strains were used as controls. all the strains were also tested for their adhesion to polystyrene. adhesion to ileostomy glycoproteins and to polystyrene varied significantly among the strains tested. lactobacillus rham ...199910196761
acute oral toxicity and bacterial translocation studies on potentially probiotic strains of lactic acid bacteria.three potentially probiotic lactic acid bacteria (lab) strains, lactobacillus rhamnosus hn001 (dr20(tm)), lb. acidophilus hn017 and bifidobacterium lactis hn019 (dr10()), have recently been identified and characterized. the present study was designed to evaluate the acute oral toxicity of these strains to mice, and also to investigate bacterial translocation and gut mucosal pathology in balb/c mice fed hn019, hn001 or hn017 for 8 consecutive days at a high dose of 10(11)cfu/mouse/day. results sh ...200010717355
effect of 8 week intake of probiotic milk products on risk factors for cardiovascular diseases.to investigate the effect of a probiotic milk product containing the culture causido(r) and of two alternative products on risk factors for cardiovascular disease in overweight and obese subjects.200010745279
genetic organization and polymorphism of the guaa gene encoding the gmp synthetase in lactobacillus rhamnosus.the guaa gene encoding gmp synthetase was cloned from a potential probiotic strain of lactobacillus rhamnosus. dna sequence and northern blot analysis indicated that (i) guaa did not belong to an guaab operonic structure, conversely to enteric bacteria, (ii) l. rhamnosus guaa seemed to be highly expressed, and (iii) genetic regulation might differ from bacillus subtilis. moreover, differences in the genetic organization of guaa allowed discrimination of some closely related l. rhamnosus strains, ...200010688693
enhancement of natural and acquired immunity by lactobacillus rhamnosus (hn001), lactobacillus acidophilus (hn017) and bifidobacterium lactis (hn019).consumption of lactic acid bacteria (lab) has been suggested to confer a range of health benefits including stimulation of the immune system and increased resistance to malignancy and infectious illness. in the present study, the effects of feeding lactobacillus rhamnosus (hn001, dr20), lactobacillus acidophilus (hn017) and bifidobacterium lactis (hn019, dr10) on in vivo and in vitro indices of natural and acquired immunity in healthy mice were examined. mice were fed daily with l. rhamnosus, l. ...200010743496
production of lactic acid by lactobacillus rhamnosus with vitamin-supplemented soybean hydrolysate.batch fermentation studies were performed to evaluate the potentials of a complex nitrogen source, soybean, as an alternative to yeast extract for the economical production of lactic acid by lactobacillus rhamnosus. an enzyme-hydrolysate of soybean meal, soytone, with an adequate supplementation of vitamins was found to be highly effective in supporting lactic acid production from glucose and lactose. the effects of seven selected vitamins: d-biotin, pyridoxine, p-aminobenzoic acid, nicotinic ac ...200010689079
citrate utilization by homo- and heterofermentative lactobacilli.citrate utilization by several homo- and heterofermentative lactobacilli was determined in kempler and mckay and in calcium citrate media. the last medium with glucose permitted best to distinguish citrate-fermenting lactobacilli. lactobacillus rhamnosus atcc 11443, lactobacillus zeae atcc 15820 and lactobacillus plantarum atcc 8014 used citrate as sole energy source, whereas in the other strains, glucose and citrate were cometabolized. some lactobacilli strains produced aroma compounds from cit ...200010772153
ability of lactobacillus and propionibacterium strains to remove aflatoxin b, from the chicken duodenum.the ability of lactobacillus rhamnosus strains gg and lc-705 to remove afb1 from the intestinal luminal liquid medium has been tested in vivo using a chicken intestinal loop technique. in this study, the gg strain of l. rhamnosus decreased afb1 concentration by 54% in the soluble fraction of the luminal fluid within 1 min. this strain was more efficient in binding afb1 compared with l. rhamnosus strain lc-705 (p < 0.05) that removed 44% of afbl under similar conditions. accumulation of afb1 into ...200010772225
a probiotic strain of l. acidophilus reduces dmh-induced large intestinal tumors in male sprague-dawley rats.probiotic bacteria strains were examined for their influence on 1,2-dimethylhydrazine (dmh)-induced intestinal tumors in 100 male sprague-dawley rats. lactobacillus acidophilus (delvo pro la-1), lactobacillus rhamnosus (gg), bifidobacterium animalis (cscc1941), and streptococcus thermophilus (dd145) strains were examined for their influence when added as freeze-dried bacteria to an experimental diet based on a high-fat semipurified (ain-93) rodent diet. four bacterial treatments were compared: l ...199910693169
comparative analysis of the genes encoding 23s-5s rrna intergenic spacer regions of lactobacillus casei-related strains.in this study, investigations into the 23s-5s rrna intergenic spacer regions (isrs) of the lactobacillus casei group were performed. a 1.6 kb fragment, from lactobacillus paracasei strain atcc 27092, containing part of the 5s rrna gene (60 bp), the 5s-23s spacer region (198 bp) and part of the 23s rrna gene (1295 bp) was cloned and sequenced (genbank no. af098107). this fragment was used as a probe to determine the rrna restriction fragment length polymorphism (rflp) patterns of nine strains bel ...200010758849
weissella confusa (basonym: lactobacillus confusus) bacteremia: a case report.infection with lactobacillus is rare, and only a handful of species have been identified as being clinically significant: lactobacillus casei, lactobacillus rhamnosus, and lactobacillus leichmannii. the literature contains one case report of bacteremia caused by weissella confusa (basonym: lactobacillus confusus), but the clinical significance of the infection was unclear. we describe a case of w. confusa bacteremia in a 46-year-old man with a history of abdominal aortic dissection and repair. t ...200111283096
structure of an extracellular polysaccharide produced by lactobacillus rhamnosus strain c83.the extracellular polysaccharide produced by lactobacillus rhamnosus strain c83 was found to be composed of d-glucose and d-galactose in a molar ratio of 2:3. the primary structure of the polysaccharide was shown by sugar analysis, methylation analysis, fabms, partial acid hydrolysis and nuclear magnetic resonance (nmr) spectroscopy to consist of a pentasaccharide repeating unit having the following structure: -->3)-alpha-d-glcp-(1-->2)-beta-d-galf-(1-->6)-alpha-d-galp-(1-->6 )-alpha-d -glcp-(1- ...199810335590
analysis of the fecal microflora of human subjects consuming a probiotic product containing lactobacillus rhamnosus dr20.the composition of the fecal microflora of 10 healthy subjects was monitored before (6-month control period), during (6-month test period), and after (3-month posttest period) the administration of a milk product containing lactobacillus rhamnosus dr20 (daily dose, 1.6 x 10(9) lactobacilli). monthly fecal samples were examined by a variety of methods, including bacteriological culture analysis, fluorescent in situ hybridization with group-specific dna probes, denaturing gradient gel electrophore ...200010831441
biosynthesis of lipoteichoic acid in lactobacillus rhamnosus: role of dltd in d-alanylation.the dlt operon (dlta to dltd) of lactobacillus rhamnosus 7469 encodes four proteins responsible for the esterification of lipoteichoic acid (lta) by d-alanine. these esters play an important role in controlling the net anionic charge of the poly (grop) moiety of lta. dlta and dltc encode the d-alanine-d-alanyl carrier protein ligase (dcl) and d-alanyl carrier protein (dcp), respectively. whereas the functions of dlta and dltc are defined, the functions of dltb and dltd are unknown. to define the ...200010781555
lactobacilli and streptococci activate nf-kappa b and stat signaling pathways in human macrophages.gram-positive bacteria induce the production of several cytokines in human leukocytes. the molecular mechanisms involved in gram-positive bacteria-induced cytokine production have been poorly characterized. in this work we demonstrate that both nonpathogenic lactobacillus rhamnosus gg and pathogenic streptococcus pyogenes (group a streptococci) induce nf-kappa b and stat dna-binding activity in human primary macrophages as analyzed by emsa. nf-kappa b activation was rapid and was not inhibited b ...200010725732
rapid identification of 11 human intestinal lactobacillus species by multiplex pcr assays using group- and species-specific primers derived from the 16s-23s rrna intergenic spacer region and its flanking 23s rrna.rapid and reliable two-step multiplex polymerase chain reaction (pcr) assays were established to identify human intestinal lactobacilli; a multiplex pcr was used for grouping of lactobacilli with a mixture of group-specific primers followed by four multiplex pcr assays with four sorts of species-specific primer mixtures for identification at the species level. primers used were designed from nucleotide sequences of the 16s-23s rrna intergenic spacer region and its flanking 23s rrna gene of membe ...200010856652
production of exopolysaccharide by lactobacillus rhamnosus r and analysis of its enzymatic degradation during prolonged fermentation.the potential of lactobacillus rhamnosus r for producing exopolysaccharide (eps) when grown on basal minimum medium supplemented with glucose or lactose was investigated. eps production by l. rhamnosus r is partially growth associated and about 500 mg of eps per liter was synthesized with both sugars. the product yield coefficient (y(eps/s)) was 3.15 (0.0315 g of eps [g of lactose](-1)) and 2.88 (0.0288 g of eps [g of glucose](-1)). it was clearly shown that the amount of eps produced declined u ...200010831403
identification of salivary lactobacillus rhamnosus species by dna profiling and a specific probe.the lactobacillus genus has been shown to be associated with the dental carious process, but little is known about the species related to the decay, although lactobacillus rhamnosus is suspected to be the most implicated species. conventional identification methods based on biochemical criteria lead to ambiguous results, since the lactobacillus species found in saliva are phenotypically close. to clarify the role of this genus in the evolution of carious disease, this work aimed to find a rapid ...200111316369
safety assessment of potential probiotic lactic acid bacterial strains lactobacillus rhamnosus hn001, lb. acidophilus hn017, and bifidobacterium lactis hn019 in balb/c mice.the general safety of immune-enhancing lactic acid bacteria (lab) strains lactobacillus rhamnosus hn001 (dr20), lb. acidophilus hn017, and bifidobacterium lactis hn019 (dr10) was investigated in a feeding trial. groups of balb/c mice were orally administered test lab strains or the commercial reference strain lb. acidophilus la-1 at 2.5 x 10(9), 5 x 10(10) or 2.5 x 10(12) colony forming units (cfu)/kg body weight/day for 4 weeks. throughout this time, their feed intake, water intake, and live bo ...200010857928
probiotic activities of lactobacillus casei rhamnosus: in vitro adherence to intestinal cells and antimicrobial properties.the interest of probiotics as remedies for a broad number of gastrointestinal and other infectious diseases has gained wide interest over the last few years, but little is known about their underlying mechanism of action. in this study, the probiotic activities of a human isolate of lactobacillus casei subsp. rhamnosus strain (lcr35) were investigated. using intestinal caco-2 cell line in an in vitro model, we demonstrated that this strain exhibited adhesive properties. the inhibitory effects of ...200111316370
analysis of promoter sequences from lactobacillus and lactococcus and their activity in several lactobacillus species.promoter-active fragments were isolated from the genome of the probiotic organism lactobacillus rhamnosus strain gg using the promoter-probe vector pnz272. these promoter elements, together with a promoter fragment isolated from the vaginal strain lactobacillus fermentum br11 and two previously defined promoters (lactococcus lactis and lactobacillus acidophilus atcc 4356 slpa), were introduced into three strains of lactobacillus. primer-extension analysis was used to map the transcriptional star ...200010896218
factors affecting the sequestration of aflatoxin by lactobacillus rhamnosus strain gg.the interaction of a potent carcinogen, aflatoxin b(1) (afb(1)), with a probiotic strain of lactic acid bacteria, lactobacillus rhamnosus strain gg (gg), has been investigated. the binding of afb(1) to gg in the late exponential-early stationary phase was studied for viable, heat-killed and acid-killed bacteria. in general, viable, heat-killed and acid-killed gg responded in a similar manner. the effects of pronase e, lipase and m-periodate on afb(1) binding and release were consistent with afb( ...200010996299
micro-organisms administered in the benefit of the host: myths and facts.to evaluate the published literature on the potential benefit of micro-organisms on the general well being of the host.200011057452
characterisation of the shrinkage of calcium alginate gel membrane with immobilised lactobacillus rhamnosus.to quantify the shrinkage of calcium alginate gel membrane as a support matrix for immobilising cells during the fermentation of lactobacillus rhamnosus, factors including time, ph, membrane thickness, and the concentrations of immobilised cells, lactic acid, glucose, and calcium chloride were examined by statistical experimental design. a plackett-burman design was used for the first screening experiment to identify the important factors which caused the divergent effects. uniform design, a pow ...200010952001
changes in the cultivable flora in deep carious lesions following a stepwise excavation procedure.this study examined the cultivable microflora before and after stepwise excavation procedures in deep carious lesions in 9 permanent teeth, categorized according to degrees of proximal surface destruction. the final excavation was performed 4-6 months after the initial treatment, which included peripheral dentine excavation and removal of the central cariogenic biomass and the superficial necrotic dentine. dentine colour and consistency were assessed by means of standardized scales before the ap ...200011093026
phage-related dna polymorphism in dairy and probiotic lactobacillus.various dna-based methods are presently being applied for identification of industrial bacterial cultures including dairy starter and probiotic strains of lactobacillus. the success of strain-specific identification depends on the power of the dna-based methods to reveal intraspecies dna polymorphism. this study reveals that all eleven arbitrarily chosen lactobacillus rhamnosus starter, laboratory and probiotic strains contain lb. rhamnosus phage lc-nu related nucleotide sequences. one of these ...200110900381
binding of aflatoxin b1 alters the adhesion properties of lactobacillus rhamnosus strain gg in a caco-2 model.lactic acid bacteria have been previously reported to possess antimycotoxigenic activities both in vitro and in vivo. the objective of this study was to investigate the effect of aflatoxin b1 on adhesion capability of lactobacillus rhamnosus strain gg using a caco-2 adhesion model. removal of aflatoxin b1 by l. rhamnosus strain gg reduced the adhesion capability of this strain from 30% to 5%. it is therefore concluded that aflatoxins may influence the adhesion properties of probiotics able to se ...200010716575
the effect of orally administered viable probiotic and dairy lactobacilli on mouse lymphocyte proliferation.four common lactobacillus strains were screened for their effects on proliferation of mouse splenic lymphocytes. mice received perorally 10(9) viable bacteria kg(-1) body weight for 7 days. lactobacillus acidophilus treatment enhanced ex vivo basal proliferation (by 43%) and b-cell response at suboptimal and optimal concentrations of lipopolysaccharide (lps) (by 27-28%). conversely, lactobacillus casei, lactobacillus gasseri and lactobacillus rhamnosus inhibited both basal proliferation (by 14-5 ...199910536299
probiotic therapy and functional foods for prevention of urinary tract infections: state of the art and science.in the past year, interest has heightened in the potential for probiotics to prevent urinary tract infection (uti). mainly, this has been due to concerns about antibiotic resistance and recognition of the scientific efficacy of probiotics. the critical factors in any successful application of probiotics to patient care are the scientific basis for selecting probiotic strains and clinical verification that they are effective against the recurrence of uti. three strains--lactobacillus rhamnosus gr ...200011095902
interleukin-10 generation in atopic children following oral lactobacillus rhamnosus gg.oral lactobacillus rhamnosus gg ingestion for 5 days to 4 weeks has been shown to alleviate clinical symptoms of gastrointestinal inflammation and atopic dermatitis. to determine whether oral lactobacillus rhamnosus gg may act by generating immunosuppressive mediator in atopic children. lactobacillus rhamnosus gg (atcc 53103) at a daily dose of 2 x 1010 cfu was added for 4 weeks to the diets of nine children (mean age, 21 months) with atopic dermatitis. blood and faecal samples were collected be ...200011122221
quantitative approach in the study of adhesion of lactic acid bacteria to intestinal cells and their competition with enterobacteria.to describe the phenomena of bacterial adhesion to intestinal cells and the competition for adhesion between bacteria, mathematical equations based on a simple dissociation process involving a finite number of bacterial receptors on intestinal cell surface were developed. the equations allow the estimation of the maximum number of lactobacillus sp. and escherichia coli cells that can adhere to caco-2 cells and intestinal mucus; they also characterize the affinity of the bacteria to caco-2 cells ...200010966378
adherence of probiotic bacteria to human intestinal mucus in healthy infants and during rotavirus infection.the concentration of fecal mucin and the adhesion of specific probiotics and their combinations in the intestinal mucus of infants during and after rotavirus diarrhea and in healthy children were determined. mucus was prepared from fecal samples from 20 infants during and after rotavirus diarrhea and from 10 healthy age-matched children. mucin concentration was determined, and the adhesion of five probiotics-lactobacillus rhamnosus gg, lactobacillus casei shirota, lactobacillus paracasei f19, la ...200111238211
protection from gastrointestinal diseases with the use of probiotics.probiotics are nonpathogenic microorganisms that, when ingested, exert a positive influence on the health or physiology of the host. they can influence intestinal physiology either directly or indirectly through modulation of the endogenous ecosystem or immune system. the results that have been shown with a sufficient level of proof to enable probiotics to be used as treatments for gastrointestinal disturbances are 1) the good tolerance of yogurt compared with milk in subjects with primary or se ...200111157353
dry sausage fermented by lactobacillus rhamnosus strains.the ability of three probiotic lactobacillus rhamnosus strains gg, e-97800 and lc-705 and one commercial pediococcus pentosaceus starter strain (control) to produce dry sausage was studied. during the fermentation process the numbers of inoculated lactic acid bacteria increased from approx. 7 log10 to 8-9 log10 cfu/g and the ph values decreased from 5.6 to 4.9-5.0. the sensory test indicated that the dry sausages fermented by l. rhamnosus lc-705 were inferior to the control sausages. the presenc ...200111252505
oral probiotics can resolve urogenital infections.we report the first clinical evidence that probiotic lactobacilli can be delivered to the vagina following oral intake. in 10 women with a history of recurrent yeast vaginitis, bacterial vaginosis (bv) and urinary tract infections, strains lactobacillus rhamnosus gr-1 and lactobacillus fermentum rc-14 suspended in skim milk and given twice daily for 14 days, were recovered from the vagina and identified by morphology and molecular typing within 1 week of commencement of therapy. in six cases of ...200111172991
probiotics in foods not containing milk or milk constituents, with special reference to lactobacillus plantarum 299v.lactic acid fermentation is the simplest and safest way of preserving food and has probably always been used by humans. species such as lactobacillus plantarum, lactobacillus rhamnosus, lactobacillus paracasei, lactobacillus acidophilus, and lactobacillus salivarius are common in the human mucosa, from the mouth to the rectum. in food, l. paracasei and l. rhamnosus are usually associated with dairy products whereas l. plantarum is found in fermented foods of plant origin. a probiotic food produc ...200111157345
influence of temperature on flavour compound production from citrate by lactobacillus rhamnosus atcc 7469.the citrate utilization by lactobacillus rhamnosus atcc 7469 was found to be temperature-dependent. the maximum citrate utilization and incorporation of [1,5-14c]citrate rate were observed at 37 degreesc. at this temperature, maximum citrate lyase activity and specific diacetyl and acetoin production (y(da%)) were observed. the high levels of alpha-acetolactate synthase and low levels of diacetyl reductase, acetoin reductase and l-lactate dehydrogenase found at 37 degreesc led to an accumulation ...200111297355
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