| cloning, sequencing and expression of the gene encoding the cell-envelope-associated proteinase from lactobacillus paracasei subsp. paracasei ncdo 151. | the gene encoding the cell-envelope-associated proteinase of lactobacillus paracasei subsp. paracasei ncdo 151 (formerly lactobacillus casei ncdo 151) was cloned and sequenced. the gene was located on the chromosome and encoded a polypeptide of 1902 amino acids. the proteinase is n-terminally cleaved upon maturation. it shows extensive homology to the lactococcus lactis subsp. cremoris wg2 proteinase. similar to the situation in lactococcus, a maturation gene was found upstream of the proteinase ... | 1992 | 1512565 |
| purification and n-terminal amino acid sequence determination of the cell-wall-bound proteinase from lactobacillus paracasei subsp. paracasei. | the cell-wall-bound proteinase from lactobacillus paracasei subsp. paracasei ncdo 151 was purified to homogeneity by anion-exchange and hydrophobic-interaction chromatography, chromatofocusing and gel-filtration. the purification resulted in a 600-700-fold increase in specific activity of the proteinase and the final yield was approximately 20%. upon chromatofocusing, two proteolytically active components, termed pro135 and pro110, were detected. pro135 had an isoelectric point of 4.2. it had an ... | 1992 | 1564442 |
| cloning of promoter-like sequences from lactobacillus paracasei subsp. paracasei cg11 and their expression in escherichia coli, lactococcus lactis, and lactobacillus reuteri. | fragments of chromosomal dna from lactobacillus paracasei subsp. paracasei cg11 (formerly lactobacillus casei cg11) capable of functioning as promoters were isolated using the broad host range, promoter-probe vector pgkv210. five such fragments designated p61, p79, p80, p116, and p144 were completely sequenced and analyzed. fragment p61 had the highest transcriptional efficiency in escherichia coli and lactobacillus reuteri whereas p80 was the most active in lactococcus lactis. in general, the o ... | 1994 | 7704831 |
| synthesis and angiotensin converting enzyme-inhibitory activity of n-[(1s)-1-carboxy-5-(4-piperidyl)pentyl]-l-alanine derivatives. | as part of a search for potent and long-lasting angiotensin converting enzyme (ace) inhibitors, various types of n-[(1s)-1-carboxy-5-(4-piperidyl)pentyl]-l-alanine derivatives (7a, 8-11) were prepared. the key synthetic intermediate, n-[(1s)-5-(1-benzyloxycarbonyl-4-piperidyl)-1- ethoxycarbonylpentyl]-l-alanine (17a), was synthesized by asymmetric reduction of the alpha-oxoester (13) with lactobacillus paracasei subsp. paracasei followed by a substitution reaction with tert-butyl l-alaninate (15 ... | 1994 | 8004703 |
| the aggregation of human platelets by lactobacillus species. | the ability to aggregate human platelets was examined for five lactobacillus rhamnosus strains and five lactobacillus paracasei subsp. paracasei strains isolated from patients with infective endocarditis (ie), 25 laboratory isolates from the same two species, and 14 strains from five other oral species, namely lactobacillus acidophilus, lactobacillus fermentum, lactobacillus oris, lactobacillus plantarum and lactobacillus salivarius. amongst the l. rhamnosus strains, platelets were aggregated by ... | 1993 | 8126421 |
| fermentation of fructans by epiphytic lactic acid bacteria. | a total of 712 strains of lactic acid bacteria isolated from forage grasses were studied for their ability to ferment fructans of phlein- as well as inulin-type. only 16 strains utilized phlein and eight of these also fermented inulin. they were identified as lactobacillus paracasei subsp. paracasei, lact. plantarum, lact. brevis and pediococcus pentosaceus. in the species lact. paracasei subsp. paracasei, all strains gave positive results, whereas the other positive strains possessed unique pro ... | 1994 | 8200866 |
| cell surface characteristics of lactobacillus casei subsp. casei, lactobacillus paracasei subsp. paracasei, and lactobacillus rhamnosus strains. | hydrophilic and electrostatic cell surface properties of eight lactobacillus strains were characterized by using the microbial adhesion to solvents method and microelectrophoresis, respectively. all strains appeared relatively hydrophilic. the strong microbial adhesion to chloroform, an acidic solvent, in comparison with microbial adhesion to hexadecane, an apolar n-alkane, demonstrated the particularity of lactobacilli to have an important electron donor and basic character and consequently the ... | 1997 | 9143109 |
| growth inhibition of staphylococcus aureus by h2o2-producing lactobacillus paracasei subsp. paracasei isolated from the human vagina. | h2o2 production by certain lactobacillus strains is one of the mechanisms that helps to regulate the vaginal ecosystem. this paper describes the kinetics of h2o2 production by two different strains of lactobacillus paracasei subsp. paracasei under different culture conditions and the effect of this metabolite on the growth of staphylococcus aureus. l. paracasei f2 produced 2.72 mmol 1-1 h2o2 while l. paracasei f28 produced 1.84 mmol l(-1), both in agitated cultures. although l. paracasei f2 prod ... | 1999 | 10076905 |
| characterization of lactic acid bacteria isolated from a thai low-salt fermented fish product and the role of garlic as substrate for fermentation. | lactic acid bacteria (lab) isolated from raw materials (fish, rice, garlic and banana leaves) and processed som-fak (a thai low-salt fermented fish product) were characterized by api 50-ch and other phenotypic criteria. lactococcus lactis subsp. lactis and leuconostoc citreum were specifically associated with fish fillet and minced fish, lactobacillus paracasei subsp. paracasei with boiled rice and weisella confusa with garlic mix and banana leaves. in addition, lactobacillus plantarum, lactobac ... | 1999 | 10100902 |
| differentiation of lactobacillus isolates from infant faeces by sds-page and rrna-targeted oligonucleotide probes. | isolates of lactobacilli from infant faeces phenotypically characterized as lactobacillus paracasei subsp. paracasei (six strains), lact. rhamnosus (six strains), lact. gasseri (three strains), lact. acidophilus (one strain) and lact. fermentum/reuteri (three strains) according to recent classification systems were subjected to sds-page of whole cell proteins and rrna-targeted oligonucleotide probe hybridization, in order to confirm the phenotypic characterization and elucidate the exact taxonom ... | 1999 | 10594716 |
| effects of lactic acid bacteria in inoculants on changes in amino acid composition during ensilage of sterile and non-sterile ryegrass. | a study was carried out on the changes occurring in the amino acid fraction of a hybrid ryegrass during ensilage in laboratory-scale silos to help to establish the relative roles of plant and microbial proteases on protein degradation in the silo. herbage treatments included (i) normal grass without treatment (ii) lambda-irradiated grass (sterile) without treatment (iii) sterile, inoculated with a strain of lactobacillus plantarum and (iv) sterile, inoculated with a strain of lactobacillus parac ... | 2000 | 11021576 |
| lactic acid bacteria from healthy oral cavity of thai volunteers: inhibition of oral pathogens. | the aims of the present study were to screen and characterize the antimicrobial lactic acid bacteria which were isolated from healthy oral cavities of thai volunteers, and to characterize their inhibiting substances. among 3790 isolates (suspected to be lactic acid bacteria) from 130 volunteers, five showed an appreciable effect against sarcina lutea atcc 9341, bacillus cereus atcc 11778, escherichia coli atcc 25922, staphylococcus aureus atcc 6538, streptococcus mutans dtmu 1, strep. salivarius ... | 2001 | 11168719 |
| strategy for manipulation of cheese flora using combinations of lacticin 3147-producing and -resistant cultures. | the aim of the present study was to develop adjunct strains which can grow in the presence of bacteriocin produced by lacticin 3147-producing starters in fermented products such as cheese. a lactobacillus paracasei subsp. paracasei strain (dpc5336) was isolated from a well-flavored, commercial cheddar cheese and exposed to increasing concentrations (up to 4,100 arbitrary units [au]/ml) of lantibiotic lacticin 3147. this approach generated a stable, more-resistant variant of the isolate (dpc5337) ... | 2001 | 11375183 |
| determination of the growth stages of lactobacillus paracasei subsp. paracasei m3 from bulgarian yellow cheese by electroconductivity. | the growth curve and the bacteriocin production by lactobacillus paracasei subsp. paracasei m3-a strain isolated from bulgarian yellow cheese, was studied using electroconductivity measurements in the culture medium (mrs). the bacteriocin produced by this bacterium inhibits strains belonging to the candida species as well as bacillus subtilis atcc 6633. the use of the direct impedance technique coupled with statistical treatment of the results for the strain's growth curve allowed the detection ... | 2001 | 11438187 |
| the growth and interaction of yeasts and lactic acid bacteria isolated from zimbabwean naturally fermented milk in uht milk. | nine yeast and four lactic acid bacterial strains, previously isolated from zimbabwean traditionally fermented milk, were inoculated into ultra-high temperature treated (uht) milk in both single and yeast-lactic acid bacteria co-culture. the lactic acid bacteria (lab) strains consisted of lactococcus lactis subsp. lactis biovar. diacetylactis c1, l. lactis subsp. lactis lc39, l. lactis subsp. lactis lc261 and lactobacillus paracasei subsp. paracasei lb11. the yeast strains used were candida kefy ... | 2001 | 11545217 |
| lactic acid bacteria and yeasts in kefir grains and kefir made from them. | in an investigation of the changes in the microflora along the pathway: kefir grains (a)-->kefir made from kefir grains (b)-->kefir made from kefir as inoculum (c), the following species of lactic acid bacteria (83-90%) of the microbial count in the grains) were identified: lactococcus lactis subsp. lactis, streptococcus thermophilus, lactobacillus delbrueckii subsp. bulgaricus, lactobacillus helveticus, lactobacillus casei subsp. pseudoplantarum and lactobacillus brevis. yeasts (10-17%) identif ... | 2002 | 11938463 |
| selection of escherichia coli-inhibiting strains of lactobacillus paracasei subsp. paracasei. | the aim of this study was to select escherichia coli-inhibiting strains among lactic acid bacteria. on the basis of phenotypical and technological characteristics, 20 strains of lactic acid bacteria were screened from a total of 225 isolates, obtained from nine samples of artisanal caprino d'aspromonte cheese, made from raw goats' milk. the antagonistic activity of these 20 strains was detected in plates against three different strains of e. coli. two strains of lactobacillus paracasei subsp. pa ... | 2002 | 12483469 |
| significant differences between lactobacillus casei subsp. casei atcc 393t and a commonly used plasmid-cured derivative revealed by a polyphasic study. | many studies on lactobacillus casei subsp. casei (l. casei) have been carried out using strain atcc 393 (plz15-). four strains of l. casei atcc 393t and three of atcc 393 (plz15-) were compared using phenotypic methods and many of the available genotyping techniques. these tests showed that strains of atcc 393t obtained from independent public type-culture collections were significantly different from the plasmid-free (plz15-) strains of atcc 393t. these findings were confirmed by sequencing the ... | 2003 | 12656154 |
| lectin-like binding of lactobacilli considered for their use in probiotical preparations for animal use. | three gut lactobacilli from piglets (lactobacillus plantarum l 5, lactobacillus paracasei l 81, lactobacillus fermentum l 670) and lactobacillus casei subsp. pseudoplantarum l.c.) from a calf were examined by microtitre plate binding assay for their lectin-like binding activity after their cultivation on rogosa agar and in mrs broth. three ecm (extracellular matrix) molecules (fetuin, porcine fibronectin and porcine mucin) were selected for this assay. additionally, the effect of heparin on the ... | 2003 | 12680274 |
| protease, peptidase and esterase activities by lactobacilli and yeast isolates from feta cheese brine. | the study of peptidase, esterase and caseinolytic activity of lactobacillus paracasei subsp. paracasei, debaryomyces hansenii and sacchromyces cerevisiae isolates from feta cheese brine. | 2003 | 12807455 |
| isolation and partial biochemical characterization of a proteinaceous anti-bacteria and anti-yeast compound produced by lactobacillus paracasei subsp. paracasei strain m3. | new proteinaceous active substance produced by lactobacillus paracasei subsp. paracasei strain m3 used as a starter for bulgarian yellow cheese was identified and studied. it displayed bactericidal and fungistatic activities. its activity was checked against over 60 bacterial and yeast strains. it was efficient against bacillus subtilis atcc 6633, several l. delbrueckii species, helicobacter pylori ncipd 230 and some yeast species, for example candida albicans, c. pseudointermedia nbimcc 1532, c ... | 2003 | 12927708 |
| six cases of lactobacillus bacteraemia: identification of organisms and antibiotic susceptibility and therapy. | six cases of bacteraemia in hospitalized patients, 5 with a depressed immune status, were caused by lactobacilli. sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole-cell proteins and api 50 ch carbohydrate patterns assigned the causative agents to the species lactobacillus rhamnosus, lactobacillus curvatus, lactobacillus delbrueckii subsp. lactis and lactobacillus paracasei subsp. paracasei. | 2003 | 12953954 |
| two distinct pathways for the formation of hydroxy fa from linoleic acid by lactic acid bacteria. | twenty-three of 86 strains of lactic acid bacteria transformed linoleic acid into hydroxy fa. two distinct conversion pathways were in operation. two strains of lactobacillus acidophilus and a strain of pediococcus pentosaceus produced 13(s)-hydroxy-9-octadecenoic acid 113(s)-oh 18:11 and 10,13dihydroxyoctadecanoic acid (10,13-oh 18:0) as main and minor products, respectively, whereas 13 strains, including l. casei subsp. casei, l. paracasei subsp. paracasei, l. rhamnosus, l. lactis subsp. cremo ... | 2003 | 14870930 |
| purification and characterization of a branched-chain amino acid aminotransferase from lactobacillus paracasei subsp. paracasei chcc 2115. | purification and characterization of an aminotransferase (at) specific for the degradation of branched-chain amino acids from lactobacillus paracasei subsp. paracasei chcc 2115. | 2004 | 14962140 |
| a mixed culture of propionibacterium jensenii and lactobacillus paracasei subsp. paracasei inhibits food spoilage yeasts. | screening for antimicrobial features of 197 propionibacteria and tests with several antifungal lactobacilli led to the development of three protective cultures containing propionibacterium jensenii sm11 and lactobacillus paracasei subsp. paracasei strain sm20, sm29 or sm63. these cultures showed inhibitory activities (up to 5 orders of magnitude) against yeasts in dairy products such as yoghurt or cheese surface at refrigerator temperatures (6 degrees c) without an influence on the quality prope ... | 2004 | 15046312 |
| screening of lactic-acid bacteria from south african barley beer for the production of bacteriocin-like compounds. | strains of lactobacillus paracasei subsp. paracasei (strain st11br), l. pentosus (strain st151br), l. plantarum (strain st13br), and lactococcus lactis subsp. lactis (strain st34br) producing bacteriocin-like peptides were isolated from barley beer produced in the western, northern and eastern provinces of south africa. the peptides (bacst11br, bacst151br, bacst13br and bacst34br) lost their activity after treatment with proteinase k, a proteinase, papain, chymotrypsin, trypsin, pepsin and prona ... | 2004 | 15530005 |
| isolation and identification of lactic acid bacteria with effect of immune protection to eschericia coli in mice. | lactic acid bacteria were isolated from an alcohol fermentation broth, and the activity as a probiotic was examined using pathogenic e. coli. thirty-six strains exhibiting good growth were isolated in the medium of concentrated mush which was a residue resulted in the alcohol distillation process. one of these strains, lactobacillus paracasei subsp. paracasei i-5, could be grown in the medium containing 8 vol% ethanol and at 45 degrees c. the characteristics were different from the type strain, ... | 2004 | 15630189 |
| characterization and antimicrobial activity of bacteriocin 217 produced by natural isolate lactobacillus paracasei subsp. paracasei bgbuk2-16. | the strain lactobacillus paracasei subsp. paracasei bgbuk2-16. which was isolated from traditionally homemade white-pickled cheese, produces bacteriocin 217 (bac217; approximately 7 kda). the onset of bac217 biosynthesis was observed in the logarithmic phase of growth, and the production plateau was reached after 9 or 12 h of incubation at 37 and 30 degrees c, respectively, when culture entered the early stationary phase. biochemical characterization showed that bac217 retained antimicrobial act ... | 2004 | 15633678 |
| fermentation of a milk-soymilk and lycium chinense miller mixture using a new isolate of lactobacillus paracasei subsp. paracasei ntu101 and bifidobacterium longum. | a milk-soymilk mixture was fermented using lactobacillus paracasei subsp. paracasei ntu101 and bifidobacterium longum bcrc11847 at different inoculum ratios (1:1, 1:2, 1:5, 2:1, and 5:1). when the inoculum ratio was 1:2, the cell numbers of both strains were balanced after 12 h of cultivation. the ph and titratable acidity were very similar at the various inoculum ratios of cultivation. the milk-soymilk mixture was supplemented with 5, 10, 15, and 20% lycium chinense miller juice and fermented w ... | 2004 | 15662546 |
| detection of antifungal properties in lactobacillus paracasei subsp. paracasei sm20, sm29, and sm63 and molecular typing of the strains. | lactobacilli isolated from different food and feed samples such as raw milk, cheese, yoghurt, olives, sour dough, as well as corn and grass silage, were screened for their antifungal activities. out of 1,424 isolates tested, 82 were shown to be inhibitory to different yeasts (candida spp. and zygosaccharomyces bailii) and a penicillium sp., which were previously isolated from spoiled yoghurt and fruits. carbohydrate fermentation patterns suggested that a substantial portion, 25%, belonged to the ... | 2005 | 15690811 |
| homofermentative lactic acid bacteria of a traditional cheese, comlek peyniri from cappadocia region. | comlek peyniri is a typical artisanal cheese in central anatolia. this type of cheese was made by using the indigenous lactic acid bacteria (lab) flora of cow or ewes' milk. majority of the samples were taken from fresh cheese because the aim was to isolate homofermentative lab. initially 661 microbial isolates were obtained from 17 cheese samples. only 107 were found to be homofermentative lab. these isolates were selected and identified by using both phenotypic and molecular methods. phenotypi ... | 2005 | 15747727 |
| phenotypic and genotypic characterization of lactobacilli isolated from spanish goat cheeses. | lactic acid bacteria from 18 spanish goat cheeses produced by seven dairies were isolated to evaluate the genetic diversity of this bacterial community. 136 representative isolates were characterized by phenotyping and randomly amplified polymorphic dna (rapd-pcr) analysis. ten species were identified with predominance of lactobacillus paracasei subsp. paracasei. the presence of l. curvatus, l. pentosus, l. cellobiosus and l. rhamnosus has not hitherto been reported in spanish goat cheeses. a hi ... | 2005 | 16014303 |
| the effects of lactobacillus-fermented milk on lipid metabolism in hamsters fed on high-cholesterol diet. | the objective of this study was to evaluate the effects of local lactobacillus strains (ntu 101 and 102) on cholesterol-lowering effects in vivo. thirty male hamsters were housed, divided into five groups, and fed on a cholesterol diet (5 g/kg diet) to induce hypercholesterolemia. milk fermented by lactobacillus paracasei subsp. paracasei ntu 101, lactobacillus plantarum ntu 102, and lactobacillus acidophilus bcrc 17010 was administrated for this study. after treatment with different fermented m ... | 2006 | 16172889 |
| lactobacillus paracasei subsp. paracasei 8700:2 degrades inulin-type fructans exhibiting different degrees of polymerization. | ten strains of lactobacilli were assessed for their capacity to degrade inulin-type fructans, which are well-known prebiotics. both oligofructose and inulin were tested. the dairy isolate lactobacillus acidophilus ibb 801 degraded only oligofructose. the human isolate lactobacillus paracasei subsp. paracasei 8700:2 degraded oligofructose and long-chain inulin and grew rapidly on both energy sources. in both cases, fractions of different degrees of polymerization were fermented. moreover, large a ... | 2005 | 16269678 |
| ribotyping of lactobacillus casei group strains isolated from dairy products. | a series of lactobacilli isolated from dairy products were characterized using biotyping and ribotyping with ecori and hindiii restriction enzymes. biotyping assigned 14 strains as lactobacillus casei, 6 strains as lactobacillus paracasei subsp. paracasei and 12 as lactobacillus rhamnosus. the obtained ribotype patterns separated all analyzed strains into two clearly distinguished groups corresponding to l. rhamnosus and l. casei/l. paracasei subsp. paracasei. the hindiii ribotypes of individual ... | 2005 | 16295661 |
| probiotic lactobacilli and bacteraemia in stockholm. | the objective of the present study was to determine the incidence of lactobacilli-induced bacteraemia in stockholm, sweden, between january 1998 and march 2004, and to identify the possible presence of probiotic strains. isolated strains were checked for growth on selective lactobacilli-agar and the biochemical profiles were determined. the lactobacilli strains were further analysed with molecular methodologies to ascertain eventual similarities with the probiotic strains lactobacillus paracasei ... | 2006 | 16709533 |
| dose-response study of probiotic bacteria bifidobacterium animalis subsp lactis bb-12 and lactobacillus paracasei subsp paracasei crl-341 in healthy young adults. | this study was performed to investigate the dose-response effects of supplementation with bifidobacterium animalis subsp lactis (bb-12) and lactobacillus paracasei subsp paracasei (crl-431) on blood lipids, recovery from feces and bowel habits. changes of the fecal microflora was analyzed in the 10(10) cfu/day probiotic and placebo group. | 2006 | 16721394 |
| potential of lactic acid bacteria isolated from specific natural niches in food production and preservation. | autochthonous strains of lactic acid bacteria (lab) have been isolated from traditionally homemade cheeses collected from specific ecological localities across serbia and montenegro. genetic and biochemical analysis of this lab revealed that they produce bacteriocins, proteinases and exopolysaccharides. lab produces a variety of antimicrobial substances with potential importance for food fermentation and preservation. apart from the metabolic end products, some strains also secrete antimicrobial ... | 2006 | 16764959 |
| [effectiveness of oral administration of lactobacillus paracasei subsp. paracasei f19 in association with vaginal suppositories of lactobacillus acidofilus in the treatment of vaginosis and in the prevention of recurrent vaginitis]. | the aim of this paper was to estimate the effectiveness of the contemporary oral administration of lactobacilllus paracasei subsp paracasei f19 in association with vaginal suppositories containing lactobacilllus acidofilus in the treatment of bacterial vaginosis and in the prevention of recurrent vaginitis. | 2006 | 16783294 |
| characterization of microflora in homemade semi-hard white zlatar cheese. | the zlatar cheese belongs to the group of traditionally homemade cheeses, which are produced from nonpasteurized cow's milk, without adding of any bacterial starter culture. changes were followed in lactic acid bacteria population and chemical composition during the ripening period of cheese up to 60 days. results showed that the percentage of lactic acid cocci was higher in raw milk and one day old cheese and their percentage was gradually decreasing, whereas the number of lactobacilli was incr ... | 2007 | 17182146 |
| lactobacillus paracasei subsp. paracasei b21060 suppresses human t-cell proliferation. | recent studies have shown that probiotics are beneficial in t-cell-mediated inflammatory diseases. the molecular mechanism by which probiotics work remains elusive, but accumulating evidence indicates that probiotics can modulate immune cell responses. since t cells express receptors for bacterial products or components, we examined whether different strains of lactobacilli directly regulate the functions of human t cells. cd4(+) t cells were isolated from blood and intestinal lamina propria (lp ... | 2007 | 17242060 |
| molecular and biochemical characterizations of human oral lactobacilli as putative probiotic candidates. | the objective of this study was to characterize the lactobacilli from the human oral cavity as a potential source of probiotic strains. | 2007 | 17311634 |
| lactic acid bacteria in marinades used for modified atmosphere packaged broiler chicken meat products. | lactic acid bacteria (lab) in some marinades commonly used in finland for modified atmosphere packaged poultry meat products were enumerated and identified to determine whether the marinades contained lab species that cause meat spoilage. the concentrations of lab in 51 marinade samples ranged from less than 100 to 8.0 x 10(5) cfu/ml. seventeen of the samples produced lab growth only after enrichment, and in five samples no growth was detected either by direct culturing or enrichment. eighty-eig ... | 2007 | 17388074 |
| dominant lactic acid bacteria and their technological properties isolated from the himalayan ethnic fermented milk products. | ethnic people of the himalayan regions of india, nepal, bhutan and china consume a variety of indigenous fermented milk products made from cows milk as well as yaks milk. these lesser-known ethnic fermented foods are dahi, mohi, chhurpi, somar, philu and shyow. the population of lactic acid bacteria (lab) ranged from 10(7) to 10(8) cfu/g in these himalayan milk products. a total of 128 isolates of lab were isolated from 58 samples of ethnic fermented milk products collected from different places ... | 2007 | 17562218 |
| molecular characterization of a novel bacteriocin and an unusually large aggregation factor of lactobacillus paracasei subsp. paracasei bgsj2-8, a natural isolate from homemade cheese. | screening the collection of natural isolates from semi-hard homemade cheese resulted in isolation and characterization of strain lactobacillus paracasei subsp. paracasei bgsj2-8. the strain bgsj2-8 harbors several important phenotypes, such as bacteriocin production, aggregation phenomenon, and production of proteinase. bacteriocin sj was purified by three-step chromatography. mass spectrometry established molecular mass of the active peptide at 5372 da. the auto-aggregation phenotype of wild-ty ... | 2007 | 17657532 |
| microbial reclamation of squid pen for the production of a novel extracellular serine protease by lactobacillus paracasei subsp paracasei tku012. | a protease-producing bacterium, strain tku012, was isolated from infant vomited milk and identified as lactobacillus paracasei subsp paracasei. strain tku012 produced protease when it was grown in a medium containing squid pen powder of marine waste. an extracellular protease was purified from culture supernatant by deae-sepharose and sephacryl s-100 chromatography. a protease, purified 77-fold to homogeneity in an overall yield of 11%, has a molecular weight of about 49 kda estimated by sds-pag ... | 2008 | 17888653 |
| preliminary characterization of lactic acid bacteria isolated from zlatar cheese. | isolation, characterization and identification of lactic acid bacteria (lab) from artisanal zlatar cheese during the ripening process and selection of strains with good technological characteristics. | 2007 | 18045397 |
| characterization of lactic acid bacteria isolated from bukuljac, a homemade goat's milk cheese. | the bukuljac cheese is traditionally homemade cheese, produced from heat-treated goat's milk without the addition of any bacterial starter culture. the presence of lactic acid bacteria (lab) in bukuljac cheese has been analyzed by using a polyphasic approach including microbiological and molecular methods such as rep-pcr with (gtg)5 primer. lactobacillus paracasei subsp. paracasei represents a dominant strain in the microflora of analyzed cheese. out of 55 gram-positive and catalase-negative iso ... | 2008 | 18177967 |
| bioconversion of squid pen by lactobacillus paracasei subsp paracasei tku010 for the production of proteases and lettuce growth enhancing biofertilizers. | a protease-producing bacterium, strain tku010, was isolated from infant vomited milk and identified as lactobacillus paracasei subsp. paracasei. a surfactant-stable protease, purified 64-fold from the third day culture supernatant to homogeneity in an overall yield of 11%, has a molecular weight of about 49,000. the enzyme degraded casein and gelatin, but did not degrade albumin, fibrin, and elastin. the enzyme activity was increased about 1.5-fold by the addition of 5mm ba(2+). however, fe(2+) ... | 2008 | 18187322 |
| casitone-dependent transcriptional regulation of the prtp and prtm genes in the natural isolate lactobacillus paracasei subsp. paracasei. | the prtp-prtm intergenic region of lactobacillus paracasei subsp. paracasei bghn 14 was cloned and sequenced. the nucleotide sequence of the prtp-prtm intergenic region in bghn 14, containing divergently orientated p(prtp) and p(prtp) promoters, was shorter by 35 bp in comparison with that in lactococci. the nucleotide sequence involved in casitone-dependent transcriptional regulation of the lactococcal prt genes was not found in the bghn14. the activity of p(prtm) in l. lactis nz9000 was very l ... | 2007 | 18450219 |
| time-dependent persistence of enhanced immune response by a potential probiotic strain lactobacillus paracasei subsp. paracasei ntu 101. | the possible time-dependent role of lactic acid bacteria (lab) in immunomodulation was investigated in balb/c mice fed daily with lactobacillus paracasei subsp. paracasei ntu 101 (10(8) colony forming units) for 3, 6, and 9 weeks, and following feeding with lactobacillus-free food for a further 7 days. we observed up-regulation of the antigen-presenting ability of dendritic cells, and expression of natural killer group-2 d (nkg2d) molecules capable of trigger natural killer cell-mediated cytotox ... | 2008 | 18809220 |
| sugar fermentation in probiotic bacteria--an in vitro study. | food supplemented with probiotic bacteria is a rapidly growing sector of the market. the aim of the present study was to evaluate and compare the acid production of selected probiotic strains available in commercial products. | 2008 | 18954354 |
| rep-pcr characterization and biochemical selection of lactic acid bacteria isolated from the delta area of egypt. | samples of raw milk and traditional dairy products were collected from different rural areas in the delta region. 170 isolates from these products were identified using repetitive genomic element-pcr (rep-pcr) fingerprinting. the identified isolates were tested for efficiency of biomass production and separation, acidifying activity, autolytic and aminopeptidase properties, antagonistic activities and exopolysaccharide production. the obtained results revealed that the lactobacillus delbrueckii ... | 2009 | 18992953 |
| atherosclerosis-preventing activity of lactic acid bacteria-fermented milk-soymilk supplemented with momordica charantia. | in this study, the milk-soymilk and milk-soymilk supplemented with momordica charantia , a common oriental vegetable possessing medicinal activities, were fermented by lactic bacteria. the objective of this study was to investigate the effects of milk-soymilk and fermented milk-soymilk with or without m. charantia on atherosclerosis in hyperlipidemic hamsters. fermented 25% milk and 75% soymilk combinations, supplemented with 1% m. charantia solution, can improve the acceptability of the ferment ... | 2009 | 19216552 |
| characterization of low-molecular-weight antiyeast metabolites produced by a food-protective lactobacillus-propionibacterium coculture. | we developed a ph-controlled batch fermentation process with separately immobilized cells of the protective coculture of lactobacillus paracasei subsp. paracasei sm20 and propionibacterium jensenii sm11 in supplemented whey permeate medium yielding cell-free supernatants with high antiyeast activity against candida pulcherrima and rhodotorula mucilaginosa. the antiyeast compounds were resistant to proteinase k and pronase e treatments and showed high heat resistance (121 degrees c for 15 min). d ... | 2008 | 19244902 |
| acid production by oral strains of candida albicans and lactobacilli. | both candida albicans and lactobacilli are common colonizers of carious lesions in children and adolescents. the purpose of this study is to compare the velocity of acid production between c. albicans and several lactobacillus species at different ph levels and concentrations of glucose. washed, pure resting-cell suspensions were obtained by culturing a total of 28 oral isolates comprising the species c. albicans, lactobacillus rhamnosus, lactobacillus paracasei paracasei, lactobacillus paracase ... | 2009 | 19246906 |
| beneficial preventive effects of gastric mucosal lesion for soy-skim milk fermented by lactic acid bacteria. | in this study, lactobacillus paracasei subsp. paracasei ntu101 and lactobacillus plantarum ntu 102 were used as starter to ferment soy-skim milk, and the optimal mixing ratio was evaluated. the influence of lactic acid bacteria (lab)-fermented soy-skim milk on mucosal integrity in a gastric mucosal lesion rat model was also investigated. after 24 h, cell densities of l. paracasei subsp. paracasei ntu 101 and l. plantarum ntu 102 fermented in 75% soy milk and 25% milk (optimal condition) were 1.2 ... | 2009 | 19358530 |
| variation in specificity of the prtp extracellular proteinases in lactococcus lactis and lactobacillus paracasei subsp. paracasei. | comparison of cell-wall-bound extracellular proteinases (ceps) from lactobacillus paracasei (lbp) ssp. paracasei natural isolates bghn14, bgar75 and bgar76 with lactococcus lactis (lcl) ssp. cremoris wg2, in their action on alpha(s1)-, beta- and kappa-casein was done. the ceps of lbp strains were able to degrade alpha(s1)- and beta-caseins and their caseinolytic specificity depended on the type of buffer used. these ceps, compared with lcl wg2, differ in four amino acid residues in small segment ... | 2009 | 19649733 |
| antigen-presenting cells exposed to lactobacillus acidophilus ncfm, bifidobacterium bifidum bi-98, and bi-504 reduce regulatory t cell activity. | the effect in vitro of six different probiotic strains including lactobacillus acidophilus ncfm, lactobacillus salivarius ls-33, lactobacillus paracasei subsp. paracasei ys8866441, lactobacillus plantarum lp-115, bifidobacterium bifidum bi-504 and bi-98 was studied on splenic enteroantigen-presenting cells (apc) and cd4(+)cd25(+) t-regulatory cells (tregs) in splenocyte-t cell proliferation assays. | 2010 | 19714743 |
| validation of the dorsal air pouch model to predict and examine immunostimulatory responses in the gut. | to validate the use of the air pouch system to predict and examine early immune responses induced by the presumptive probiotics lactobacillus paracasei subsp. paracasei b112, dc205, dc215 and dc412 strains in the gut mucosa. | 2010 | 20002910 |
| effect of the administration of lactobacillus paracasei subsp. paracasei ntu 101 on peyer's patch-mediated mucosal immunity. | the role of lactic acid bacteria in gut mucosal immunity was investigated by comparing the enhanced effects in the peyer's patches and spleen of balb/c mice fed daily with lactobacillus paracasei subsp. paracasei ntu 101 for 3 to 9 weeks. after feeding with lactobacillus, the percentage of cd4+ t cells in both peyer's patches and the spleen was significantly increased; however, expression of cd 154 molecules, which play a pivotal role in cell-to-cell communication, on cd4+ t cells and the percen ... | 2010 | 20417727 |
| construction of a new shuttle vector and its use for cloning and expression of two plasmid-encoded bacteriocins from lactobacillus paracasei subsp. paracasei bgsj2-8. | a new shuttle-cloning vector, pa13, was constructed and successfully introduced into escherichia coli, lactobacillus and lactococcus strains. it showed high segregational and structural stability in all three hosts. the natural plasmid psj2-8 from l. paracasei subsp. paracasei bgsj2-8 was cloned into pa13 using bamhi to obtain the construct, pb5. sequencing and in silico analysis of pb5 revealed fifteen open reading frames (orf). plasmid psj2-8 harbours genes encoding the production of two bacte ... | 2010 | 20439125 |
| immunomodulating activity of lactobacillus paracasei subsp. paracasei ntu 101 in enterohemorrhagic escherichia coli o157h7-infected mice. | the present study investigated the immunomodulating activity of lactobacillus paracasei subsp. paracasei ntu 101 in enterohemorrhagic escherichia coli o157:h7-infected balb/c mice. mice were given l. paracasei subsp. paracasei ntu 101 (10(8) colony-forming units) for 7 days, before and after the challenge with e. coli o157:h7. feeding lactobacillus for 7 days resulted in an increased postchallenge weight gain and lower cumulative morbidity rates. we observed the upregulation of dendritic cells, ... | 2010 | 20942489 |
| co-culturing of lactobacillus paracasei subsp. paracasei with a lactobacillus delbrueckii subsp. delbrueckii mutant to make high cell density for increased lactate productivity from cassava bagasse hydrolysate. | to increase the productivity of lactic acid, a co-culture of lactobacilli was made by mixing 1:1 ratio of lactobacillus paracasei subsp. paracasei and a fast growing l. delbrueckii subsp. delbrueckii mutant. the culture was embedded on to polyurethane foam (puf) cubes as a biofilm and used for fermentation. in order to prevent the cell leakage, the puf cubes were further entrapped in calcium cross-linked alginate. the maximum lactic acid production using a high cell density free culture was >38 ... | 2010 | 20972788 |
| widely distributed lysogeny in probiotic lactobacilli represents a potentially high risk for the fermentative dairy industry. | prophages account for most of the genetic diversity among strains of a given bacterial species, and represent a latent source for the generation of virulent phages. in this work, a set of 30 commercial, collection and dairy-isolated lactobacillus casei group strains were used. a species-specific pcr assay allowed a reclassification, mainly of strains previously considered lactobacillus casei, into either lactobacillus paracasei or lactobacillus rhamnosus. all the strains were induced with mitomy ... | 2010 | 21131090 |
| conversion of squid pen by a novel strain lactobacillus paracasei subsp. paracasei tku010, and its application in antimicrobial and antioxidants activity. | tku010 was isolated from infant vomited milk and identified as lactobacillus paracasei subsp. paracasei. tku010 had desirable properties concerning its ability to withstand adverse conditions in the gastrointestinal tract. the hydrolysate of casein enhanced the growth of tku010 most obviously (17.20-18.25 od(660)), followed by the hydrolysate of spp (16.00-15.06 od(660)). incubating with spp, both the culture supernatant of tku010 on the first day and the fourth day showed inhibitory activities ... | 2010 | 21282904 |
| antihypertensive effects of lactobacillus-fermented milk orally administered to spontaneously hypertensive rats. | products fermented with lactic acid bacteria may show antihypertensive effects via substances such as angiotensin i-converting enzyme inhibitor (acei) and ?-aminobutyric acid (gaba). it was previously found that milk fermented with lactobacillus paracasei subsp. paracasei ntu 101 (101fm) or lactobacillus plantarum ntu 102 (102fm) has acei and gaba activities. this study aimed to investigate the antihypertensive effects of 101fm and 102fm orally administered to spontaneously hypertensive rats (sh ... | 2011 | 21446645 |
| lactobacillus saniviri sp. nov. and lactobacillus senioris sp. nov., isolated from human faeces. | two gram-positive-staining strains, yit 12363t and yit 12364t, isolated from human faeces were rod-shaped, non-motile, asporogenous, facultative anaerobic and did not exhibit catalase activity. comparative analyses of 16s rrna, phes and rpoa gene sequence demonstrated that the novel strains were members of the genus lactobacillus. on the basis of 16s rrna gene sequence similarity, the type strains of lactobacillus casei (95.3%), lactobacillus paracasei subsp. paracasei (95.6%), lactobacillus par ... | 2011 | 21515698 |
| immunomodulatory and antioxidant potential of lactobacillus exopolysaccharides. | background: immunomodulation by probiotic microorganisms has become a topic of increasing interest in food microbiology. polysaccharides are broadly used in the food industry as gelling, thickening, stabilizing, or emulsifying agents. some probiotics such as lactic acid bacteria also produce exopolysaccharides that stimulate macrophage production of cytokines. the aim of this study was to characterize the effects of exopolysaccharides of lactobacillus paracasei subsp. paracasei ntu 101 (101ep) a ... | 2011 | 21560134 |
| isolation and phenotypic characterization of lactobacillus casei and lactobacillus paracasei bacteriophage-resistant mutants. | aims: to isolate and characterize bacterial strains derived from lactobacillus casei and lactobacillus paracasei strains and resistant to phage mlc-a. methods and results: two of nine assayed strains rendered resistant mutants with recovery efficiencies of 83% (lact. paracasei atcc 27092) and 100% (lact. casei atcc 27139). dna similarity coefficients (rapd-pcr) confirmed that no significant genetic changes occurred while obtaining resistant mutants. neither parent nor mutant strains spontaneou ... | 2011 | 21599814 |
| screening of species-specific lactic acid bacteria for veal calves multi-strain probiotic adjuncts. | the selection of promising specific species of lactic acid bacteria with potential probiotic characteristics is of particular interest in producing multi species-specific probiotic adjuncts in veal calves rearing. the aim of the present work was to select and evaluate in vitro the functional activity of lactic acid bacteria, bifidobacterium longum and bacillus coagulans strains isolated from veal calves in order to assess their potential use as multi species-specific probiotics for veal calves. ... | 2011 | 21619939 |
| antiosteoporotic effects of lactobacillus -fermented soy skim milk on bone mineral density and the microstructure of femoral bone in ovariectomized mice. | osteoporosis is a major skeletal disease associated with loss of estrogen in postmenopausal women. in this study, lactobacillus paracasei subsp. paracasei ntu 101 (ntu 101f) and lactobacillus plantarum ntu 102 (ntu 102f) were used as starters to ferment soy skim milk. this was then used as a nutritional supplement for 8 weeks to ovariectomized (ovx) mice. this study reveals that soy skim milk fermented with lactobacilli can increase the contents of aglycone isoflavones, soluble calcium, and vita ... | 2011 | 21668014 |
| effect of bioactive compounds in lactobacilli-fermented soy skim milk on femoral bone microstructure of aging mice. | background: soy skim milks fermented with lactobacilli contain various phytochemicals such as isoflavones and peptides. we used lactobacilli-fermented soy skim milk as a nutritional supplement for 6 weeks to investigate its anti-osteoporosis effect in 13-month-old female balb/c aging mice. freeze-dried powder of soy skim milk fermented by lactobacillus paracasei subsp. paracasei ntu 101 (ntu 101f) and l. plantarum ntu 102 (ntu 102f) were used in this study. results: the trabecular bone volumes i ... | 2011 | 21815163 |
| characterization of certain bacterial strains for potential use as starter or probiotic cultures in dairy products. | the present work was aimed at characterizing 12 strains of lactic acid bacteria (lab) to obtain improved potential starter or probiotic cultures that could be used for making dairy products from ewe's milk and cow's milk. eight strains with antimicrobial properties, isolated from ewe's milk and from cheese made from ewe's and/or cow's milk, were studied. they were identified as enterococcus faecalis (five strains), lactococcus lactis subsp. cremoris, leuconostoc mesenteroides, and lactobacillus ... | 2011 | 21819671 |
| accelerated production of dry fermented sausage. | the scope of this paper is to review work connected with accelerated ripening of dry fermented sausages by addition of proteolytic enzymes. an overview of the following topics is given: practical sausage experiments with addition of various proteinases of bacterial origin, including data from sensory, biochemical and gc/ms analyses; biochemical and genetic characterization of the enzyme shown to be most useful in these experiments, the serine proteinase from lactobacillus paracasei subsp. paraca ... | 1996 | 22060654 |
| Beneficial effects of Lactobacillus paracasei subsp. paracasei NTU 101 and its fermented products. | It is well-known that probiotics have a number of beneficial health effects in humans and animals, including the reduction of symptoms in lactose intolerance and enhancement of the bioavailability of nutrients. Probiotics have showed to possess antimutagenic, anticarcinogenic and hypocholesterolemic properties. Further, they were also observed to have antagonistic actions against intestinal and food-borne pathogens, to decrease the prevalence of allergies in susceptible individuals and to have i ... | 2011 | 22159887 |
| beneficial effects of a strain of lactobacillus paracasei subsp. paracasei in staphylococcus aureus-induced intestinal and colonic injury. | the aim of this study was to investigate the in vitro and in vivo anti-staphylococcal activity of a lactic acid bacterial strain and its effect on the intestinal histological damage caused by staphylococcus aureus infection. | 2011 | 21963346 |
| characterization and purification of a bacteriocin from lactobacillus paracasei subsp. paracasei bmk2005, an intestinal isolate active against multidrug-resistant pathogens. | a strain of lactobacillus paracasei subsp. paracasei bmk2005 isolated from healthy infant faeces has shown a remarkable antibacterial activity against 32 bacterial pathogenic strains of human clinical isolates. among them, 13 strains belonging to species of escherichia coli, citrobacter freundii, citrobacter diversus, klebsiella oxytoca, enterobacter cloacae and pseudomonas aeruginosa were resistant to cefotaxime (ctx) and ceftazidime (caz), and 4 strains of staphylococcus aureus were resistant ... | 2011 | 22805936 |