| leukemoid reaction in epidermal squamous cell carcinoma. | there have been no previous concrete reports of leukemoid reactions associated with squamous cell carcinoma originating in cutaneous tissue. here we report a case of epidermal squamous cell carcinoma of the sacral region and an associated leukemoid reaction. the tumor invaded deeply and destroyed both the sacrum and coccyx. the white blood cell count was greater than 20,000/mm3. after resection of the tumor, white blood cells transiently decreased, but did not fall under 10,000/mm3. post-operati ... | 1996 | 8854586 |
| in vitro activity of gemifloxacin (sb 265805) against anaerobes. | gemifloxacin mesylate (sb 265805), a new fluoronaphthyridone, was tested against 359 recent clinical anaerobic isolates by the national committee for clinical laboratory standards reference agar dilution method with supplemented brucella blood agar and an inoculum of 10(5) cfu/spot. comparative antimicrobials tested included trovafloxacin, levofloxacin, grepafloxacin, sparfloxacin, sitafloxacin (du-6859a), penicillin g, amoxicillin clavulanate, imipenem, cefoxitin, clindamycin, and metronidazole ... | 1999 | 10471570 |
| comparative activity of moxifloxacin in vitro against obligately anaerobic bacteria. | the antimicrobial activity of moxifloxacin and seven other antibiotics (four of them quinolones) against 292 strains of obligately anaerobic bacteria was assessed employing a broth microdilution technique performed in wilkens-chalgren broth. mic50/mic90 values (mg/l) for moxifloxacin were as follows: bacteroides fragilis (n = 62) 0.25/2, bacteroides ovatus (n = 70) 1/4, bacteroides vulgatus (n = 29) 0.25/1, bacteroides thetaiotaomicron (n = 17) 2/2, bacteroides caccae (n = 11) 1/2, prevotella sp ... | 2000 | 10795599 |
| in vitro activity of telithromycin (hmr 3647) and seven other antimicrobial agents against anaerobic bacteria. | we assessed the in vitro activity of telithromycin (hmr 3647) and seven other antimicrobials against 292 strains of obligately anaerobic bacteria. mics were determined with the microdilution technique and wilkins-chalgren broth according to din 58940-83. mic50/mic90s (mg/l) for telithromycin were 4/4 for bacteroides fragilis, bacteroides ovatus and bacteroides thetaiotaomicron, 2/4 for fusobacterium spp. and bilophila wadsworthia, 2/2 for bacteroides caccae, 1/4 for bacteroides vulgatus, 0.25/4 ... | 2000 | 10882699 |
| advances in clinical laboratory tests for inflammatory bowel disease. | inflammatory bowel disease (ibd) is a generic term that refers to crohn's disease and chronic ulcerative colitis (uc). the cd and uc are considered to be distinct forms of ibd; but there is a subgroup of cd with a uc-like presentation. the genetic factors play a significant role in ibd. ibd is associated with a strong familial pattern. recent studies support the hypothesis that ibd patients have a dysregulated immune response to endogenous bacteria in the gastrointestinal tract. the serologic re ... | 2003 | 12927679 |
| fermentation of pectin and glucose, and activity of pectin-degrading enzymes in the rabbit caecal bacterium bacteroides caccae. | to compare fermentation pattern in cultures of bacteroides caccae supplied with pectin and glucose, and identify enzymes involved in metabolism of pectin. | 2004 | 15214734 |
| lessons learned from the anaerobe survey: historical perspective and review of the most recent data (2005-2007). | the rationale and lessons learned through the evolution of the national survey for the susceptibility of bacteroides fragilis group from its initiation in 1981 through 2007 are reviewed here. the survey was conceived in 1980 to track emerging antimicrobial resistance in bacteroides species. | 2010 | 20067390 |
| mucosa-associated bacteria in two middle-aged women diagnosed with collagenous colitis. | to characterize the colon microbiota in two women histologically diagnosed with collagenous colitis using a culture-independent method. | 2012 | 22529692 |
| human-specific fecal bacteria in wastewater treatment plant effluents. | the objective of this study was to identify fecal bacteria able to persist after wastewater treatment and that could be used as indicators of human fecal contamination. in a first step, the diversity of bacteroidales, clostridiaceae, bifidobacterium, and bacillus-streptococcus-lactobacillus cluster (bsl) was analysed using a fingerprint technique (ce-sscp) and 16s rdna libraries in waters collected at the end of the treatment process in different urban wastewater treatment plants. for each group ... | 2010 | 19945729 |
| [bacteria isolated from surgical infections and its susceptibilities to antimicrobial agents--special references to bacteria isolated between april 2006 and march 2007]. | tendency of isolated bacteria from infections in abdominal surgery during the period from april 2006 to march 2007 were investigated in a multicenter study in japan, and the following results were obtained. in this series, 474 strains including 23 strains of candida spp. were isolated from 170 (75.2%) of 226 patients with surgical infections. two hundred and twenty-six strains were isolated from primary infections, and 224 strains were isolated from postoperative infections. from primary infecti ... | 2008 | 18814799 |
| fecal calprotectin levels and serological responses to microbial antigens among children and adolescents with inflammatory bowel disease. | noninvasive, sensitive, and specific tools for early identification of chronic inflammatory bowel disease (ibd) are needed for clinical practice. the aim was to identify new noninvasive test combinations for characterization of ibd in children and adolescents by comparing serological responses to microbial antigens and fecal calprotectin, a new promising marker for intestinal inflammation. | 2009 | 18618670 |
| elevated serum anti-saccharomyces cerevisiae, anti-i2 and anti-ompw antibody levels in patients with suspicion of celiac disease. | expression of anti-saccharomyces cerevisiae antibodies (asca) identifies patients and individuals at risk for crohn's disease and has also been reported in 40-60% of celiac disease (cd) cases, suggesting a role of host response to enteric microbiota in the development of inflammatory lesions. in this prospective study in patients with suspicion of cd, we evaluate the frequency and association of asca to serological responses for other host microbial targets formally associated with crohn's disea ... | 2008 | 18496744 |
| elevated serum anti-i2 and anti-ompw antibody levels in children with ibd. | bacteria are implicated as important factors in the pathogenesis of inflammatory bowel disease (ibd). the aim of this study was to seek evidence of possible bacterial targets of the immune response related to ibd in children. | 2006 | 16670528 |
| rapid identification of the species of the bacteroides fragilis group by multiplex pcr assays using group- and species-specific primers. | we report a rapid and reliable two-step multiplex polymerase chain reaction (pcr) assay to identify the 10 bacteroides fragilis group species - bacteroides caccae, b. distasonis, b. eggerthii, b. fragilis, b. merdae, b. ovatus, b. stercoris, b. thetaiotaomicron, b. uniformis and b. vulgatus. these 10 species were first divided into three subgroups by multiplex pcr-g, followed by three multiplex pcr assays with three species-specific primer mixtures for identification to the species level. the pr ... | 2003 | 12757940 |
| colonic bacteria express an ulcerative colitis panca-related protein epitope. | bacteria are a suspected pathogenic factor in inflammatory bowel disease, but the identity of the relevant microbial species remains unresolved. the panca autoantibody is associated with most cases of ulcerative colitis (uc) and hence reflects an immune response associated with the disease process. this study addresses the hypothesis that panca identifies an antigen(s) expressed by bacteria resident in the human colonic mucosa. libraries of colonic bacteria were generated using aerobic and anaer ... | 2000 | 10678972 |
| inflammatory pseudotumor of the liver associated with extrahepatic infection. | we present two cases that support an infectious cause of inflammatory pseudotumor of the liver. each patient had sepsis due to chronic abdominal abscesses, along with a liver mass. both patients had exploratory celiotomy, and histologic examination of the liver mass revealed inflammatory pseudotumor of the liver. evidence of a mixed bacterial/actinomyces infection was noted in hepatic tissue sections in one patient, and bacteroides caccae was cultured directly from the liver biopsy in the other ... | 1997 | 9003818 |
| comparative activities of eight quinolones against members of the bacteroides fragilis group. | the in vitro activities of five new quinolones (clinafloxacin [ci-960 or pd-127391], bay y 3118, e-4868, e-5065, and e-5068) against 100 bacteroides fragilis group bacterial isolates were compared with those of ciprofloxacin, ofloxacin, and sparfloxacin. overall, e-5068 was the most active in vitro (mic for 90% of isolates tested [mic90], 0.25 microgram/ml); this was followed by clinafloxacin and bay y 3118 (mic90, 0.5 microgram/ml). ciprofloxacin, sparfloxacin, and ofloxacin were the least acti ... | 1994 | 8092852 |
| [necrotizing fasciitis after a cat bite: a case report]. | we report on a previously healthy 56-year-old woman who was referred to our hospital with fever and progressive left foot pain. she had been bitten by a cat 7 days previously, and cephalosporins had been prescribed for treatment. however, her clinical symptoms deteriorated, and physical examination on admission was compatible with necrotizing fasciitis. treatment with ampicillin-sulbactam and clindamycin was initiated. in addition, immediate surgical debridement was performed, resulting in thera ... | 2013 | 23713332 |
| [bacteria isolated from surgical infections and its susceptibilities to antimicrobial agents - special references to bacteria isolated between april 2011 and march 2012]. | bacteria isolated from surgical infections during the period from april 2011 to march 2012 were investigated in a multicenter study in japan, and the following results were obtained. in this series, 785 strains including 31 strains of candida spp. were isolated from 204 (78.8%) of 259 patients with surgical infections. five hundred and twenty three strains were isolated from primary infections, and 231 strains were isolated from surgical site infection. from primary infections, anaerobic gram-ne ... | 2014 | 25796741 |
| probiotic bifidobacterium longum alters gut luminal metabolism through modification of the gut microbial community. | probiotics are well known as health-promoting agents that modulate intestinal microbiota. however, the molecular mechanisms underlying this effect remain unclear. using gnotobiotic mice harboring 15 strains of predominant human gut-derived microbiota (hgm), we investigated the effects of bifidobacterium longum bb536 (bb536-hgm) supplementation on the gut luminal metabolism. nuclear magnetic resonance (nmr)-based metabolomics showed significantly increased fecal levels of pimelate, a precursor of ... | 2015 | 26315217 |
| intestinal microbiota distinguish gout patients from healthy humans. | current blood-based approach for gout diagnosis can be of low sensitivity and hysteretic. here via a 68-member cohort of 33 healthy and 35 diseased individuals, we reported that the intestinal microbiota of gout patients are highly distinct from healthy individuals in both organismal and functional structures. in gout, bacteroides caccae and bacteroides xylanisolvens are enriched yet faecalibacterium prausnitzii and bifidobacterium pseudocatenulatum depleted. the established reference microbial ... | 2016 | 26852926 |
| a microfluidics-based in vitro model of the gastrointestinal human-microbe interface. | changes in the human gastrointestinal microbiome are associated with several diseases. to infer causality, experiments in representative models are essential, but widely used animal models exhibit limitations. here we present a modular, microfluidics-based model (humix, human-microbial crosstalk), which allows co-culture of human and microbial cells under conditions representative of the gastrointestinal human-microbe interface. we demonstrate the ability of humix to recapitulate in vivo transcr ... | 2016 | 27168102 |
| generation of genome-scale metabolic reconstructions for 773 members of the human gut microbiota. | genome-scale metabolic models derived from human gut metagenomic data can be used as a framework to elucidate how microbial communities modulate human metabolism and health. we present agora (assembly of gut organisms through reconstruction and analysis), a resource of genome-scale metabolic reconstructions semi-automatically generated for 773 human gut bacteria. using this resource, we identified a defined growth medium for bacteroides caccae atcc 34185. we also showed that interactions among m ... | 2017 | 27893703 |
| early microbial markers of celiac disease. | seroreactivity against the saccharomyces cerevisiae (asca), pseudomonas fluorescens-associated sequence (i2), and bacteroides caccae tonb-linked outer membrane protein (ompw) has been detected in celiac disease patients with small-bowel mucosal atrophy. levels of these antibodies decrease during a gluten-free diet, but their functions and time of appearance in celiac disease are not known. we aimed to search for evidence of possible microbial targets of the immune responses in the early-stage ce ... | 2014 | 24518796 |
| bacteroides stercorirosoris sp. nov. and bacteroides faecichinchillae sp. nov., isolated from chinchilla (chincilla lanigera) faeces. | strains of gram-negative anaerobic rods were isolated from chinchilla (chinchilla lanigera) faeces, and three strains, st161t, st33 and st37t, were investigated taxonomically. based on the phylogenetic analyses and specific phenotypic characteristics, the three strains belonged to the genus bacteroides. phylogenetic analyses of their 16s rrna gene sequences revealed that strain st161t formed a distinct line of descent, and the highest sequence similarity to st161t was found with strain st33 (98. ... | 2011 | 21724955 |
| design of species-specific oligonucleotide probes for the detection of bacteroides and parabacteroides by fluorescence in situ hybridization and their application to the analysis of mouse caecal bacteroides-parabacteroides microbiota. | aims: to develop species-specific monitoring techniques for rapid detection of bacteroides and parabacteroides inhabiting the mouse intestine by fluorescence in situ hybridization. methods and results: the specificity of oligonucleotide probes was evaluated by fluorescence whole-cell hybridization. oligonucleotide probes specific for each species hybridized only with the target bacteria. using these probes, caecal bacteroides-parabacteroides microbiota of conventional mice and specific pathoge ... | 2011 | 21535330 |
| application of quantitative real-time pcr for rapid identification of bacteroides fragilis group and related organisms in human wound samples. | our goal was to establish a quantitative real-time pcr (qrt-pcr) method to detect bacteroides fragilis group and related organisms from clinical specimens. compared to conventional anaerobic culture, qrt-pcr can provide accurate and more rapid detection and identification of b.-áfragilis group and similar species. b.-áfragilis group and related organisms are the most frequently isolated anaerobic pathogens from clinical samples. however, culture and phenotypic identification is quite time-consum ... | 2011 | 21439390 |
| [bacteria isolated from surgical infections and their susceptibility to antimicrobial agents--special reference to bacteria isolated between april 2007 and march 2008]. | bacteria isolated from infections in abdominal surgery during the period from april 2007 to march 2008 were investigated in a multicenter study in japan, and the following results were obtained. in this series, 707 strains including 24 strains of candida spp. were isolated from 181 (79.0%) of 229 patients with surgical infections. three hundred and ninety-five strains were isolated from primary infections, and 288 strains were isolated from postoperative infections. from primary infections, anae ... | 2009 | 19860320 |
| serological responses to microbial antigens in celiac disease patients during a gluten-free diet. | immunoglobulin a (iga) autoantibodies to tissue transglutaminase (ttg) are commonly used for screening and diagnosing of celiac disease (cd). seroreactivity for anti-saccharomyces cerevisiae antibody (asca) and bacterial antigens have also been detected in cd patients. the aim of this study was to examine prospectively serologic responses to microbial targets in adult cd patients at the time of diagnosis and during a gluten-free diet (gfd). further, we wanted to evaluate whether these serologic ... | 2009 | 18987962 |
| molecular cloning of a bacteroides caccae tonb-linked outer membrane protein identified by an inflammatory bowel disease marker antibody. | commensal enteric bacteria are a required pathogenic factor in inflammatory bowel disease (ibd), but the identity of the pertinent bacterial species is unresolved. using an ibd-associated panca monoclonal antibody, a 100-kda protein was recently characterized from an ibd clinical isolate of bacteroides caccae (p2lc3). in this study, consensus oligonucleotides were designed from 100-kda peptides and used to identify a single-copy gene from the p2lc3 genome. sequence analysis of the genomic clone ... | 2001 | 11553542 |