| nucleotide sequence of the genome of a citrus isolate of olive latent virus 1. | the 3699 nt genome of olive latent virus 1 (olv-1), described years ago from southern italy as a putative sobemovirus, was completely sequenced. olv-1 genomic rna was not polyadenylated and had a structure virtually identical to that of species of the necrovirus rather than the sobemovirus genus. five open reading frames (orfs) were identified, of which the 5'-proximal encoded a 23k protein and ended with an amber codon whose readthrough could yield a putative 82k product. this polypeptide had e ... | 1996 | 8678829 |
| the complete genome sequence of a new necrovirus isolated from olea europaea l. | the complete nucleotide sequence of a virus isolated from olea europaea l. (gp isolate), previously identified as an isolate of tobacco necrosis virus d (tnv-d) based on its coat protein sequence, was determined. the viral rna genome consists of 3683 nucleotides and contains five open reading frames. the putative rna-dependent rna polymerase shows 91.2% amino acid identity with that of an isolate of olive latent virus 1 (olv-1) and the coat protein reveals highest sequence identity with that of ... | 2004 | 15592885 |
| subcellular localization and immunodetection of movement proteins of olive latent virus 1. | polyclonal sera raised to escherichia coli-expressed movement proteins encoded by orf 3 (p8k) and orf 4 (p6k) of olive latent virus 1, were used for their immunodetection in infected nicotiana benthamiana plants. in subfractionated locally infected tissues 4 days post inoculation (d.p.i.) that were analysed by western blot, p8k was found in the fast-sedimenting fractions p1 and p30 containing membranous material and/or cell organelles and, likely, the fibrous structures mentioned below, but not ... | 2005 | 15747053 |
| synthesis of infectious transcripts of olive latent virus 1: genes required for rna replication and virus movement. | genomic rna of olive latent virus 1 (olv-1) contains five open reading frames (orfs) encoding proteins of 23, 82, 8, 6 and 30 (cp) kda. a full-length cdna copy of olv-1rna was prepared and cloned in a low-copy-number vector (pmuc-19) downstream of or t7 rna polymerase promoter. transcripts derived from this template, denoted pmuc-olv, were highly infectious when inoculated in local and systemic hosts and infected tissues contained virus-like particles. genes required for replication and virus mo ... | 1999 | 10446644 |
| a degenerate pair of primers for simultaneous detection of four alpha- and betanecroviruses. | the high infection levels due to olive latent virus 1 (olv-1), olive mild mosaic virus (ommv) (alphanecrovirus) and tobacco necrosis virus d (tnv-d) (betanecrovirus) in portuguese olive orchards prompted us to develop a rapid pcr-based assay for the simultaneous detection of these viruses aimed at the sanitary selection and marketing of plant material in compliance with european union regulations. a pair of degenerate oligonucleotide primers, parrdrp5' and parcoat3' was designed based on conserv ... | 2014 | 25116202 |
| genetic diversity of the coat protein of olive latent virus 1 isolates. | the cp gene variability among 21 olive latent virus 1 (olv-1) isolates obtained from different hosts and locations and at different times was assessed. amplicons obtained by rt-pcr were cloned, and at least 10 sequences from each isolate were analyzed and compared. olv-1 sequences available in genbank were included. the encoded cps consisted of 270 amino acids, except those of isolates g1s and c7 (269 aa) and g6 (271 aa). comparison of cp genomic sequences of the isolates under study showed very ... | 2014 | 24352437 |
| molecular characterisation of the full-length genome of olive latent virus 1 isolated from tomato. | olive latent virus 1 (olv-1) is a species of the necrovirus genus. so far, it has been reported to infect olive, citrus tree and tulip. here, we determined and analysed the complete genomic sequence of an isolate designated as cm1, which was collected from tomato plant in the wielkopolska region of poland and represents the prevalent isolate of olv-1. the cm1 genome consists of monopartite single-stranded positive-sense rna genome sized 3,699 nt with five open reading frames (orfs) and small int ... | 2011 | 21181333 |
| monoclonal antibody to olive latent virus 1. | | 2006 | 17177614 |
| the role of the c-terminal region of olive latent virus 1 coat protein in host systemic infection. | a full-length cdna clone of olive latent virus 1 (olv-1), a member of the genus necrovirus, family tombusviridae, was subjected to site-directed mutagenesis, and coat protein gene mutants were constructed. a mutant clone, denoted delta3297, was obtained by deleting the nucleotide in position 3297, thus inducing a frameshift and replacing the last 49 amino acids of the viral coat protein (cp) by a shorter sequence of 39 amino acids. this mutant was viable, stable, able to synthesize a smaller cp, ... | 2006 | 16699830 |
| complete nucleotide sequence of an olive latent virus 1 isolate from olive trees. annotated sequence record. | | 2005 | 16132177 |
| viral properties, primary structure and phylogenetic analysis of the coat protein of an olive latent virus 1 isolate from olea europaea l. | an olive latent virus 1 isolate designated gm6, obtained from a portuguese olive tree, was characterized and the coat protein gene sequenced and analysed. the purified virus particles showed to be isometric with ca. 30 nm in diameter and contained a single-stranded rna species with ca. 3.7 kb. the dsrna profile obtained from infected tissues showed three major species with ca. 3.7, 1.5 and 1.3 kbp. sds-page analysis revealed a major peptide with an apparent molecular mass of 32 kda identified as ... | 2005 | 15681070 |