| a chromosome map of the european stone fruit yellows phytoplasma. | a physical map of the european stone fruit yellows phytoplasma strain gsfy1 chromosome was constructed using pfge-purified genomic dna from diseased tobacco and tomato plants. the map was generated with single and double digestions of the chromosome with smai, bsshii, apai, bamhi and xhoi restriction endonucleases and the fragments were resolved by pfge. reciprocal double digestions were used to locate 26 restriction sites on the chromosome. southern blot analysis was also used to assist in the ... | 2001 | 11320124 |
| identifying risk factors for european stone fruit yellows from a survey. | abstract european stone fruit yellows (esfy) is becoming a major economic problem for prunus growers in europe. the causal agent ("candidatus phytoplasma prunorum") and its vector (cacopsylla pruni) have been identified, but the present knowledge of the risk factors for this disease relies, at best, on specific experiments. to assess the relative significance of several factors correlated with esfy incidence in the field, an exhaustive survey was performed on apricot and japanese plum orchards i ... | 2006 | 18943755 |
| a rapid protocol of crude rna/dna extraction for rt-qpcr detection and quantification of 'candidatus phytoplasma prunorum'. | many efforts have been made to develop a rapid and sensitive method for phytoplasma and virus detection. taking our cue from previous works, different rapid sample preparation methods have been tested and applied to candidatus phytoplasma prunorum ('ca. p. prunorum') detection by rt-qpcr. a duplex rt-qpcr has been optimized using the crude sap as a template to simultaneously amplify a fragment of 16s rrna of the pathogen and 18s rrna of the host plant. the specific plant 18s rrna internal contro ... | 2016 | 26742106 |
| general phytoplasma detection by a q-pcr method using mycoplasma primers. | phytoplasmas and mycoplasmas are bacteria belonging to the class mollicutes. in this study, a fine tuning of quantitative polymerase chain reaction (qpcr) with a universal mycoplasma primer pair (gpo3f/mgso) targeting the 16s rrna gene was carried out on phytoplasmas. the dissociation curves of dnas from catharanthus roseus phytoplasma-infected micropropagated shoots and from phytoplasma field-infected plant samples showed a single peak at 82.5 °c (±0.5) specifically detecting phytoplasmas belon ... | 2017 | 28558918 |
| molecular test to assign individuals within the cacopsylla pruni complex. | crop protection requires the accurate identification of disease vectors, a task that can be made difficult when these vectors encompass cryptic species. here we developed a rapid molecular diagnostic test to identify individuals of cacopsyllapruni (scopoli, 1763) (hemiptera: psyllidae), the main vector of the european stone fruit yellows phytoplasma. this psyllid encompasses two highly divergent genetic groups that are morphologically similar and that are characterized by genotyping several micr ... | 2013 | 23977301 |