| [preparation of an insulin-like substance from molluscs and its antiserum]. | a substance with insulin-like activity was obtained from visceral organs (excluding foot, gills and mantle) of the freshwater bivalve molluscs unio pictorum and anodonta cygnea. injecting this substance to guinea pigs, antiserum was also obtained. using the obtained antiserum in immunostaining experiments, the cells which produce insulin-like substance were identified in the intestinal epithelium of the midgut in a. cygnea. | 1979 | 390928 |
| [the presence of enterovirus in species of shellfish and fish]. | enteroviruses were isolated from 12% of the digestive tract samples collected from anodonta cygnea (9.4%) and dreysena polymorpha (13.2%) shell species and from 8.4% of the branchiae sample from fish species rutilus rutilus, perca fluviatilis and silurus glanis. the isolated belonged to the following antigenic types: echo 2 and 7, coxsackie b 2, 4 and 6, and poliovirus 1 and 2. | 1990 | 2171625 |
| [certain biochemical characteristics of an insulin-like substance from the bivalve mollusk anodonta cygnea]. | an insulin-like substance (ils) was isolated from the visceral organs of the bivalve mollusc anodonta cygnea by chromatography on a sulfocationite cu-23 and purified by reverse phase liquid chromatography. ils was shown to be made up to several fractions with mr ranging from 9 to 20 kda which have identical amino acid composition but different hydrophobicity and n-terminal amino acids. it was supposed that the heterogeneity of ils fractions is due to its genetical or posttranslational polymorphi ... | 1991 | 1912075 |
| [comparative analysis of the glycoproteins of the mineralized tissues of several species of animals]. | studies have been made on carbohydrate components of glycoproteids from normally mineralized tissues of different animals. experiments were made on the scales of the fish leuciscus idus, on shells of hen eggs, on shells of the molluscs modiolus modiolus, anodonta cygnea, rapana thomasiana, bithynia tentaculata, on bone tissue, the enamel and dentine from cows, pigs, dogs and albino rats. in the glycoproteids studied, hexoses, fucoses, ketone sugars, hexosamines, uronic and sialic acids were foun ... | 1978 | 716704 |
| [a method of determining the concentration of thiamine]. | the rate of the production of thiazole, the first product of the reaction, catalyzed by thiaminase i (ec 2.5.1.2) from anodonta cygnea muscles is characterized by an initial flash-up explained by a considerable difference in the rates of the enzyme-substrate complex formation and its further transformation. basing on this phenomenon, a new method for measuring the total thiamine in vitro and in biological material has been developed. optimal conditions for thiamine measurements in the blood and ... | 1989 | 2474699 |
| cytotoxic activity in the coelomic fluid of the annelid eisenia foetida sav. | the annelid eisenia foetida not only causes hemolysis of red blood cells of several vertebrate species, but also has a toxic effect on a variety of cell types, such as chicken fibroblasts, guinea-pig polymorphonuclear leukocytes and insect hemocytes. however, it has no influence on the vitality of the coelomocytes of lumbricus terrestris and other lumbricides, nor on the hemocytes of the snail helix pomatia, the mussels anodonta cygnea and unio tumidus, free cells of the turbellarian euplanaria ... | 1987 | 3571568 |
| diamine oxidase in blood plasma in some vertebrates and anodonta cygnea before and after injection of heparin. | | 1968 | 4981028 |
| studies on the human red cell agglutinins of the swan mussel (anodonta cygnea). | | 1969 | 5403957 |
| [comparative study of the immunological properties of insulins and insulin-like substances of vertebrates and invertebrates]. | comparative studies have been made on immunological properties of insulins (bovine, porcine and fish--oncorhynchus gorbuscha, o. keta) and insulin-like substances of the mollusk anodonta cygnea and starfish asterias rubens in homologous and heterologous for these hormones radio-immune systems. it was concluded that each of these systems reveals in the insulins and insulin-like substances investigated the identical antigenic determinants. at the same time, low affinity of the hormones to heterolo ... | 1983 | 6190333 |
| [lysosomal proteolytic system of eukaryotes in the process of phylogenetic development]. | significant differences have been found between the spectra of lysosomal proteinases in animals from different taxonomic groups, i.e. hydra oligactis. digesia tigrina, anodonta cygnea, salmo irideus, rana ridibunda, gallus bankiva, rattus norvegicus. in lower organisms, which exhibit mainly intracellular digestion (hydrozoa, turbellaria), lysosomal proteolytic system is presented almost exclusively by cathepsin d, whereas in higher animals the quota of cathepsin d activity decreases (4-10 times) ... | 1982 | 7043960 |
| involvement of the adenylyl cyclase signaling system in the action of insulin and mollusk insulin-like peptide. | involvement of the adenylyl cyclase signaling system in the mechanism of action of the mammalian insulin and epidermal growth factor as well as of insulin-like peptide isolated from the bivalve mollusk anodonta cygnea has been studied. it was shown for the first time that insulin and insulin-like peptide exert in vitro the gtp-dependent stimulating action on the adenylyl cyclase activity. epidermal growth factor has an analogous effect. effectiveness of the peptides decreased in the order insuli ... | 1995 | 8590382 |
| on the tyrosine kinase mechanism of the novel effect of insulin and insulinlike growth factor i. stimulation of the adenylyl cyclase system in muscle tissues. | for the first time, insulinlike growth factor i (igf-i), like insulin (pertseva et al., comp biochem physiol 112: 689-695, 1995), was shown to exercise a gtp-dependent stimulating action on adenylyl cyclase (ac; ec 4.6.1.1.) activity in the muscle tissues (membrane fraction) of mammal (rat) and mollusc (anodonta cygnea). by studying the mechanism of the effect of peptides with selective inhibitors of tyrosine kinase activity, tyrphostin 47 (rg50864, 3,4-dihydroxy-alpha-cyanothiocinnamamide) and ... | 1996 | 8951345 |
| on the mechanism of relaxin action: the involvement of adenylyl cyclase signalling system. | the molecular mechanism of relaxin action was studied taking into account the evolutionary relationship of the peptides belonging to the insulin superfamily and using the authors' previous data on the involvement of the adenylyl cyclase (ac) signalling system in the action of insulin and related peptides. human relaxin 2 (10(-12)-10(-8) m) has been shown to cause a dose-dependent activating effect on ac in the human myometrium (+370%), in rat skeletal muscles (+117%) and the smooth foot muscles ... | 1999 | 10235632 |
| bioindicators for toxicity assessment of effluents from a wastewater treatment plant. | the discharge of effluents and toxic compounds into aquatic systems represents a growing environmental problem involving an impact on water ecology and potential effect on human health. most municipal wastewaters are complex mixtures; their complexity led as to carry out a hazard assessment using chemical analyses and biological tests. this study investigates biochemical alteratiobns in two sentinel organisms, the anodonta cygnea mussel and the xenopus laevis frog exposed for different lengths o ... | 1998 | 9839402 |
| a dual role of protein kinase c in insulin signal transduction via adenylyl cyclase signaling system in muscle tissues of vertebrates and invertebrates. | further decoding of a novel adenylyl cyclase signaling mechanism (acsm) of the action of insulin and related peptides detected earlier (pertseva et al. comp biochem physiol b biochem mol biol 1995;112:689-95 and pertseva et al. biochem pharmacol 1996;52:1867-74) was carried out with special attention given to the role of protein kinase c (pkc) in the acsm. it was shown for the first time that transduction of the insulin signal via the acsm followed by adenylyl cyclase (ac, ec 4.6.1.1) activation ... | 2001 | 11322932 |
| [the role of protein kinase c in insulin signal transduction via adenylyl cyclase signaling mechanism]. | activation of proteinkinase c with diacylglycerol or phorbol-12-myristate-13-acetate in the rat muscle membrane or anodonta cygnea mollusc blocks the insulin stimulating signal to adenylyl cyclase via tyrosinekinase type receptor. the same occurs with stimulating effect of biogenic amines to adenylyl cyclase via serpentine type receptor. transduction of the inhibitory signal induced with isoproterenol to adenylyl cyclase remained unchanged in case of the proteinkinase c activation. the findings ... | 2001 | 11601154 |
| [effect of synthetic cationic peptides on activation of the adenylyl cyclase signaling system by biogenic amines in muscle tissue of molluscs and rats]. | the hormone-sensitive adenylyl cyclase signaling system (acs), made of serpentine receptor, heterotrimeric g-protein and enzyme adenylyl cyclase (ac), regulates a wide spectrum of growth and metabolic processes in the cell. molecular mechanisms of functional coupling of acs components still remain obscure. we examined the influence of synthetic cationic peptides ac-ala-his(ala)2-his-ala-nh2 (i), ac-ala-his-(ala)3-his-(ala)2-his-ala-nh2 (ii), and ac-(pro)2-his-(ala)2-his-(ala)3-his-(ala)2-his-ala ... | 2001 | 11517665 |
| the role of abiotic factors and pesticide levels on enzymatic activity in the freshwater mussel anodonta cygnea at three different exposure sites. | natural variation in abiotic factors, such as temperature and ph, probably influence the activity of enzymes used as potential biomarkers in bivalve mollusks to assess environmental contamination in the field. changes in levels of an enzymatic biomarker may thus merely reflect natural variation in the annual physiological cycle of a species rather than exposure to contaminants. to investigate this issue, we documented the relationship between pesticide levels in water and three different enzymat ... | 2003 | 12781840 |
| study of the functional organization of a novel adenylate cyclase signaling mechanism of insulin action. | in this study we continued decoding the adenylate cyclase signaling mechanism that underlies the effect of insulin and related peptides. we show for the first time that insulin signal transduction via an adenylate cyclase signaling mechanism, which is attended by adenylate cyclase activation, is blocked in the muscle tissues of the rat and the mollusk anodonta cygnea in the presence of: 1) pertussis toxin, which impairs the action of the inhibitory gtp-binding protein (gi); 2) wortmannin, a spec ... | 2002 | 11970732 |
| comparative study of biological activity of insulins of lower vertebrates in the novel adenylyl cyclase test-system. | the biological activity of insulins of lower vertebrates (teleosts-oncorhynchus gorbuscha, scorpaena porcus, chondrosteans-acipenser guldenstaedti and cyclostomates-lamperta fluviatilis) was studied and compared with that of standard pig insulin. the determination of biological activity was made using the novel adenylyl cyclase (ac) test-system based on the adenylyl cyclase signaling mechanism (acsm) of insulin action discovered earlier by the authors. the biological activity of insulins was est ... | 2003 | 14599718 |
| [effect of c-terminal peptide of g-protein alfa(s)-subunit on regulation of adenylate cyclase and protein kinase a activities by biogenic amines and glucagon in mollusc and rat muscles]. | the peptide synthesised by us: 387-394-amide (10(-7)-10(-4) m), in a dose dependent manner decreases activities of adenyl cyclase and proteinkinase a evoked by serotonine and glucagon in smooth muscles of the freshwater bivalve mollusc anodonta cygnea and that evoked by beta-agonist isoproterenol--in the rat skeletal muscles. even in concentration 10(-7) m, the peptide completely eliminates potentiation of the hormones' stimulating effect on adenylyl cyclase activity with the non-hydrolizable an ... | 2003 | 14758620 |
| accumulation and depuration of the cyanobacterial toxin cylindrospermopsin in the freshwater mussel anodonta cygnea. | cylindrospermopsin (cyn) is a toxic alkaloid produced by several genera of freshwater cyanobacteria. this compound has been implicated in outbreaks of human sickness and the death of domestic and wild animals. given that several of the cyanobacterial genera known to produce cyn are common components of the phytoplankton of freshwaters including aquaculture facilities, we studied the accumulation of cyn in the freshwater mussel (swan mussel) anodonta cygnea. anodonta were exposed to cyn-producing ... | 2004 | 15019478 |
| identification of the multidrug resistance-associated protein (mrp) related gene in red mullet (mullus barbatus). | multixenobiotic resistance mechanism (mxr) in aquatic organisms is mediated by the activity of the p-glycoprotein (pgp) transporter that binds and actively effluxes different chemicals out of cell. in addition to the pgp, several other, non-pgp transport proteins have been recently identified in different human and animal tissues. given their characteristics and tissue distribution we hypothesized that members of the so-called multidrug resistance-associated protein (mrp) family may be expressed ... | 2004 | 15178032 |
| accumulation and depuration of cyanobacterial paralytic shellfish toxins by the freshwater mussel anodonta cygnea. | the increasing frequency by which the production of paralytic shellfish toxins (pst) by freshwater bloom-forming cyanobacteria is being noticed world-wide raises the possibility of pst bioaccumulation by freshwater mussels. this study evaluates pst accumulation and depuration by the freshwater mussel anodonta cygnea exposed over a 14-day period to high densities (mean = 1.4 x 10(9) cells1(-1), s.d. = 0.29 x 10(9) cellsl(-1)) of the toxic cyanobacterium aphanizomenon issatschenkoi (corresponding ... | 2004 | 15177951 |
| [purification and ligand-receptor analysis of insulin-related peptides from pedal ganglion of the mollusc anodonta cygnea]. | six insulin-related peptides (irps) from pedal ganglions of the molluscs anodonta cygnea have been isolated and purified by reverse-phase chromatography. each peptide (designated as irp8-irp13) showed its own retention time on the hplc column. the testing of irps in radioreceptor systems specific for insulin and insulin growth factor-i (igf-i) showed their ability to bind to both types of receptors. the concentration of irps, producing a 50% inhibition of porcine 125i-insulin binding with rat li ... | 2004 | 15344890 |
| [dissociation action of cationic peptides with hydrophobic radicals on functional coupling between serpentine type receptors and gtp-binding proteins]. | the coupling of hormone-activated receptor and heterotrimeric g protein is an important step of the signal transduction through adenylyl cyclase signal system (acs). the numerous literature data and own results show that g protein-interacting regions, that are localized in cytoplasmic loops of receptors, have considerable positive charge, can form amphiphilic alpha-helices and are tightly associated with the membrane. we studied the influence of model cationic peptides on both basal and stimulat ... | 2004 | 15214172 |
| role of phosphatidylinositol-3-kinase and protein kinase c zeta in the adenylate cyclase signal mechanism of action of relaxin in muscle tissues of rats and mollusks. | we showed that phosphatidylinositol-3-kinase and protein kinase c zeta are involved in the adenylate cyclase signal mechanism of relaxin action. a selective inhibitor of phosphatidylinositol-3-kinase wortmannin blocked the stimulatory effect of relaxin on adenylate cyclase in rat skeletal muscles and anodonta cygnea smooth muscles. antibodies against protein kinase c zeta abolished the relaxin-induced stimulation of adenylate cyclase in rat muscles, but not in mollusk muscles. our results indica ... | 2004 | 15665948 |
| effects of the c-terminal peptide of the alphas subunit of the g protein on the regulation of adenylyl cyclase and protein kinase a activities by biogenic amines and glucagon in mollusk and rat muscles. | the c-terminal parts of the a subunits of heteromeric g proteins play an important role in the functional linkage of g proteins with receptors of the serpentine type. the present report describes studies of the effects of the c-terminal octapeptide 387-394 of the alphas subunit of the mammalian g protein on the transmission of the hormonal signal via the hormone-sensitive adenylyl cyclase signal system, whose major components are receptors of the serpentine type, g proteins, and the enzymes aden ... | 2005 | 15779331 |
| [the study of the multifactorial anthropogenic effect on the ecosystems of the industrial reservoirs of "maiak" industrial complex]. | the analysis of the ecological situation of the southern urals industrial water reservoirs of the nuclear fuel cycle enterprise, "mayak" pa is represented. the study was held in the 80s - early 90s. the subjects of the study were: a cooling water reservoir--kysyl-tash lake (r-2) as well as a radioactive waste storage reservoir (r-10). irtyash lake, which is a drinking water reservoir for the city of ozyorsk and alabuga and kazhakul lakes, located on the boundary of the eastern urals radioactive ... | 2006 | 16579551 |
| [molecular mechanisms of action of dendrons, containing 48-60 sequence of hiv-1 tat-protein, on the functional activity of the adenylyl cyclase signaling systems]. | for the aims of studying molecular mechanisms of functioning of adenylyl cyclase signaling systems (acs), we investigated the influence of synthetic polycationic peptides of the star-like structure (dendrons), containing 48-60 sequence of hiv-1 tat-protein, on the functional activity of acs components in smooth muscles of the mollusc anodonta cygnea and in rat skeletal muscles. it has been shown that the following peptides (gly-arg-lys-lys-arg-arg-gln-arg-arg-arg-pro-pro-gln)2-lys-epsilonahx(= e ... | 2004 | 15704884 |
| [study of molecular mechanisms of the relaxin action on adenylyl cyclase signaling system using synthetic peptides derived from the relaxin receptor lgr7]. | the peptide hormone relaxin in dose-dependent manner stimulates adenylyl cyclase activity in the rat tissues (brain striatum, heart and skeletal muscles) and the muscle tissues of invertebrates--bivalve mollusk anodonta cygnea and earthworm lumbricus terrestris. adenylyl cyclase stimulating effect of the hormone is most expressed in striatum and heart muscles of rats. for identification of the type ofrelaxin receptors, participating in the realization of this effect of the hormone, the peptides ... | 2006 | 16869281 |
| [comparative study of molecular mechanisms of natural and synthetic polycationic peptides action on the activity of the adenylyl cyclase signaling system]. | the molecular mechanisms of action of natural and synthetic polycationic peptides, forming amphiphilic helices, on the heterotrimeric g-proteins and enzyme adenylyl cyclase (ac), components of hormone-sensitive ac system, were studied. it is shown that synthetic peptides c-epsilonahx-wkk(c10)-kkk(c10)-kkkk(c10)-ykk(c10)-kk (peptide i) and (grgdsgrkkrrqrrrppq)2-k-epsilonahx-c(acm)(peptide ii) in dose-dependent manner stimulate the basal ac activity, inhibit forskolin-stimulated ac activity and de ... | 2006 | 16892855 |
| studies on a pmca-like protein in the outer mantle epithelium of anodonta cygnea: insights on calcium transcellular dynamics. | early studies on the outer mantle epithelium (ome) cells of the freshwater bivalve anodonta cygnea (linnaeus, 1758) revealed high ionic calcium concentrations by electrophysiological methods and subsequently a high tendency to reach an intracellular toxic condition. this toxicity could be neutralized by specific mechanisms in the cytosol of ome cells of a. cygnea. the present immunocytochemistry studies of ome cells by light and transmission electron microscopy (tem) clearly showed a positive re ... | 2008 | 17712564 |
| studies of the molecular mechanisms of action of relaxin on the adenylyl cyclase signaling system using synthetic peptides derived from the lgr7 relaxin receptor. | the peptide hormone relaxin produces dose-dependent stimulation of adenylyl cyclase activity in rat tissues (striatum, cardiac and skeletal muscle) and the muscle tissues of invertebrates, i.e., the bivalve mollusk anodonta cygnea and the earthworm lumbricus terrestris, adenylyl cyclase stimulation being more marked in the rat striatum and cardiac muscle. our studies of the type of relaxin receptor involved in mediating these actions of relaxin involved the first synthesis of peptides 619-629, 6 ... | 2007 | 17763990 |
| [streptozocin model of diabetes mellitus in the mollusc anodonta cygnea: functional state of the adenylyl cyclase mechanisms of action of peptides of the insulin superfamily and their effect on carbohydrate metabolism enzymes]. | in terms of development of evolutionary biomedicine using invertebrate animals as models for study of molecular grounds of various human diseases, for the first time the streptozocin (st) model of insulin-dependent diabetes in the mollusc anodonta cygnea has been developed. this model is based on the following authors' data: (1) redetection of insulin-related peptides (irp) in mollusk tissues: (2) discovery of the adenylyl cyclase signal mechanism (acsm) of action of insulin and other peptides o ... | 2007 | 18265556 |
| [structural and functional organization of the adenylyl cyclase signaling mechanism of insulin-like growth factor 1 revealed in the muscle tissue in vertabrates and invertebrates]. | based on the earlier discovered by the authors adenylyl cyclase signaling mechanisms (acsm) of action of insulin and relaxin, the study was performed of the presence a similar action mechanism of another representative of the insulin superfamily--the insulin-like growth factor 1 (igf-1) in the muscle tissues of vertebrates (rat) and invertebrates (mollusc). for the first time there was detected participation of acsm in the igf-1 action, including the six component signaling cascade: receptor tyr ... | 2008 | 18959207 |
| [structural and functional characteristics of the adenylyl cyclase signaling system regulated by biogenic amines and peptide hormones in the muscle of a worm lumbricus terrestris]. | it has been shown for the first time that biogenic amines (catecholamines and tryptophane derivatives) stimulate dose-dependently activity of adenylyl cyclase (ac) and gtp-binding of g-proteins in muscle of the cutaneous-muscle bag of the earthworm lumbricus terrestris. by efficiency of their stimulating action on the ac activity, biogenic amines can be arranged in the following sequence: octopamine > tyramine > tryptamine = serotonin > dopamine > isoproterenol = adrenalin. the sequence of effic ... | 2008 | 18959208 |
| [properties of the adenylate cyclase system in the muscle tissue of the mollusk anodonta cygnea]. | the adenylate cyclase system in the muscular tissue of anodonta cygnea has been studied. a stimulating effect of serotonin, guanine nucleotides and sodium fluoride is found as well as a dependence of the catalytic activity of adenylate cyclase on magnesium and manganese ions. the mollusk enzyme and the vertebral one do not differ in principle in their functional properties. only serotonin out of the tested biogenic amines (epinephrine, norepinephrine, dopamine, serotonin) stimulates the adenylat ... | 2012 | 3629724 |
| association between environmental microbiota and indigenous bacteria found in hemolymph, extrapallial fluid and mucus of anodonta cygnea (linnaeus, 1758). | bivalves filter and accumulate large numbers of microorganisms present in the harvesting water. a complete understanding of the balance between anodonta cygnea and the microbiota present in their surrounding environment remains incomplete. therefore, the aim of this study was to quantify and identify the indigenous bacteria in the biological fluids of a. cygnea collected from mira lagoon in northern portugal. the results showed vibrio metschnikovii and aeromonas sobria as the dominant groups. th ... | 2010 | 20349058 |
| effect of some insecticides on the periodic activity of the fresh-water mussel (anodonta cygnea l.). | the effect of the insecticides hungaria l-7, dimecron-50, thimet-10 g and satox 20 wsc containing lindane, phosphamidon, phorate and trichlorfon, respectively, as active ingredient was investigated on the survival and pumping behaviour of the fresh-water mussel anodonta cygnea l. the shortening of active periods was used as indicator of the sublethal effects. hungaria l-7, thimet-10 g and satox 20 wsc caused significant inhibition of the filtering activity, while dimecron-50 was less effective. ... | 2000 | 89770 |
| the micronucleus assay in anodonta cygnea for the detection of drinking water mutagenicity. | a micronucleus test in gill cells of the freshwater mussel anodonta cygnea has been proposed for the detection of drinking water genotoxicity. animals were exposed for 28 days to a drinking water sample and collected every week. highly significant increases in spontaneous mn frequency were observed at each sampling, especially after 13 days of exposure. as positive control 2 doses of mytomicin c (mmc) were used (10(-8) and 10(-7) m). a second experiment was performed at a municipal waterworks in ... | 2008 | 2266975 |
| adenosine phosphate concentrations and carbohydrate consumption in the tissues of anodonta cygnea l. (mollusca, pelecypoda) under normal and anoxic conditions. | | 2015 | 4784597 |
| neurotransmitter uptake by the central nervous system of fresh-water mussel (anodonta cygnea l): i. in vitro uptake of serotonin, dopamine and noradrenaline. | the accumulation of serotonin, dopamine and noradrenaline in the cerebral, pedal and visceral ganglia is mediated by a high-affinity (uptake 1) and a low-affinity (uptake 2) system. this uptake mechanism is dependent on time and temperature. the monoamine uptake systems in the cerebral, pedal and visceral ganglia are not identical. at low external serotonin concentration, the cerebral ganglia display twice the rate of uptake 1 as compared to the visceral and pedal ganglia. | 2010 | 2878546 |
| [the paramyosin function in glycerinated muscle fibers during blocking with antibodies of its contacts with structural proteins]. | antibodies to paramyosin (apm) induce a partial decrease of the isometric tension in glycerinated fibres of the anodonta cygnea catch muscle in the presence of atp and ca2+; the myofibrillar mg2+ atpase increases concomitantly. assumedly paramyosin inhibits the cross-bridges unlocking, retaining them mechanically in a locked state. the fibres of barnacle giant muscle in an atp deficient solution respond to apm by transient isometric tension development. a model for the participation of paramyosi ... | 2014 | 4052471 |
| 2,4-dichlorophenoxyacetic acid alters intracellular ph and ion transport in the outer mantle epithelium of the bivalve anodonta cygnea. | bivalve molluscs, due to their sedentary mode of life and filter-feeding behavior, are very susceptible to pollutant bioaccumulation and used as sentinel organisms in the assessment of environment pollution. herein we aimed to determine the in vivo, ex vivo and in vitro effects of 2,4-dichlorophenoxyacetic acid (2,4-d), a widely used herbicide, in anodonta cygnea shell growth mechanisms. for that, we evaluated the effect of 2,4-d (100 μm) exposure on the transepithelial short-circuit current (is ... | 2014 | 24854203 |
| biomineralization studies on cellulose membrane exposed to biological fluids of anodonta cygnea. | the present work proposes to analyse the results obtained under in vitro conditions where cellulose artificial membranes were incubated with biological fluids from the freshwater bivalve anodonta cygnea. the membranes were mounted between two half 'ussing chambers' with different composition solutions in order to simulate epithelial surfaces separating organic fluid compartments. the membrane surfaces were submitted to two synthetic calcium and phosphate solutions on opposite sides, at ph 6.0, 7 ... | 2014 | 24710792 |
| proteomic profiling of cytosolic glutathione transferases from three bivalve species: corbicula fluminea, mytilus galloprovincialis and anodonta cygnea. | suspension-feeding bivalves are considered efficient toxin vectors with a relative insensitivity to toxicants compared to other aquatic organisms. this fact highlights the potential role of detoxification enzymes, such as glutathione transferases (gsts), in this bivalve resistance. nevertheless, the gst system has not been extensively described in these organisms. in the present study, cytosolic gsts isoforms (cgst) were surveyed in three bivalves with different habitats and life strategies: corb ... | 2014 | 24473139 |
| bioaccumulation of selected metals in bivalves (unionidae) and phragmites australis inhabiting a municipal water reservoir. | urbanization can considerably affect water reservoirs by, inter alia, input, and accumulation of contaminants including metals. located in the course of river cybina, maltański reservoir (western poland) is an artificial shallow water body built for recreation and sport purposes which undergoes restoration treatment (drainage) every 4 years. in the present study, we demonstrate an accumulation of nine metals (cd, co, cr, cu, fe, mn, ni, pb, zn) in water, sediment, three bivalve species (anodonta ... | 2014 | 24407963 |
| effects of non-steroidal estrogen diethylstilbestrol on ph and ion transport in the mantle epithelium of a bivalve anodonta cygnea. | freshwater bivalves are used as sentinel organisms to detect pollutants effects in the aquatic environment due to their sedentary nature, filter-feeding behaviour. we aimed to determine the in vivo, ex vivo and in vitro influence of diethylstilbestrol (des), a widely used synthetic non-steroidal estrogen and endocrine disruptor, in anodonta cygnea shell growth mechanisms. for that, in vivo exposure to des (0.75μm) during 15 days, in vitro and ex vivo exposure of outer mantle epithelium (ome) cel ... | 2013 | 23953926 |
| [regulation of adenylyl cyclase signaling system by insulin, biogenic amines, and glucagon at their separate and combined action in the muscle membranes of the mollusc anodonta cygnea]. | in smooth muscles of mollusc anodonta cygnea, hormones produce regulatory effects on the adenylyl cyclase (ac) signaling system via receptors of the serpentine (biogenic amine, isoproterenol, glucagon) and of tyrosine kinase (insulin) types. intracellular mechanisms of their action are interconnected. use of hormones, their antagonists, and pertussis toxin at the combined action of insulin and biogenic amines or of glucagon on the ac activity allows revealing possible intersection points in mech ... | 2015 | 23789396 |
| main partitioning criteria for the characterization of the health status in the freshwater mussel anodonta cygnea from spontaneously polluted area in western ukraine. | the aim of this study was to appreciate the consequences of spontaneous human activity for freshwater mollusks in the generally ecologically sustainable area in western ukraine. for this, bivalve mollusk, anodonta cygnea, at three sites, with mixed agricultural and municipal activities (a), close to a municipal water inlet (f) and the cooling pond of a nuclear power plant (n), were studied in spring, summer, and autumn. the set of parameters included the characteristics of oxidative stress (acti ... | 2012 | 22764078 |
| [population-related peculiarities of molecular stress-responsive systems of bivalve mollusk under the effect of tetrazine pesticide]. | tetrazine pesticides are widely used for the treatment of crops in most eu countries and usa. however, data about the effect of environmentally realistic concentrations of biocides on the molecular stress response system in non-target organisms are absent. the aim of our study was the comparison of adaptive capability of bivalve mollusk anodonta cygnea from two populations under the effects of commercial pesticide apollo in terms of biochemical parameters of the digestive gland. the differences ... | 2015 | 22679763 |
| evaluation of biotargeting and ecotoxicity of co²⁺-containing nanoscale polymeric complex by applying multi-marker approach in bivalve mollusk anodonta cygnea. | cobalt (co(2+)) is present in many nanoscaled materials created for various applications. the key goal of our study was to develop sensitive approaches for assessing the bio-risks associated with using novel co(2+)-containing nanoscaled polymeric complex (co-nc). freshwater bivalve mollusk anodonta cygnea (unionidae) was subjected to 14 d action of the developed co-nc, as well as of co(2+) applied in the corresponding concentration (50 μg l(-1)) or polymeric substance (ps). all experimental grou ... | 2012 | 22464855 |
| [evaluation of biological effects of cobalt-nanocomposites with the use of biochemical markers of bivalve mollusk anodonta cygnea]. | intensive implementation of nanomaterials requires development of novel methods for evaluation of their potential ecotoxicity. the aim of our study was to identify specific characteristics of the effect of cobalt-nanocomposite (co-nc) on the molecular stress-responsive system in the digestive gland of bivalve mollusk anodonta cygnea. nanocomposite was synthesized by mixing alcohol solution of copolymer n-vinylpirrolidone, 5-(tret-butylperoxy)-5-methyl-1-hexene-3-yne and dimethylaminoethylmetacry ... | 2015 | 22276427 |
| [ecological and radio-ecological effects from long-term use of the lake kyzyl-tash as a cooling reservoir by the nuclear fuel cycle facility]. | this review introduces long-term study findings on ecological and radiation induced regime of the water reservoir - lake kyzyl-tash (r-2) - used as a heat sink of nuclear-power reactors in the southern urals from 1948 through 2008. it was exhibited that water reservoir exploitation by the nuclear fuel cycle facility "mayak" pa resulted in hydrological, thermal, hydrochemical and radiological ecosystem regimes changes. the central radioactive substances depot in the water reservoir was determined ... | 2016 | 20968056 |
| vulnerability of biomarkers in the indigenous mollusk anodonta cygnea to spontaneous pollution in a transition country. | the aim of this study was to estimate the sensitivity of biomarkers of stress and exposure in the bivalve mollusk anodonta cygnea to spontaneous anthropogenic activities typical for the western ukraine. three sites were examined during spring, summer and autumn: an agricultural site (a); the cooling pond of nuclear power plant (n) and a forestry close to the municipal water inlet (f). common temporal changes of a battery of biochemical markers in the gills and hemolymph and morphological charact ... | 2010 | 20817257 |
| morphological characterization and size of hemocytes in anodonta cygnea. | the anodont (anodonta cygnea) constitutes one of the most important bivalves along of anzali lagoon. in last decade, anodont have suffered a high degree of mortality. description of the morphological characterization of hemocytes is a prerequisite to further exploring the causes of death in bivalves, therefore in this study; the circulating hemocytes of the a. cygnea in anzali lagoon were identified. at first, two types of hemocytes were recognized, granulocytes and agranulocytes, were identifie ... | 2009 | 19328807 |
| heavy metal concentrations in the soft tissues of swan mussel (anodonta cygnea) and surficial sediments from anzali wetland, iran. | concentrations of cadmium, copper, and lead were determined in surficial sediments and the soft tissues (foot and gills) of swan mussel anodonta cygnea from two sampling sites in anzali wetland, which is an internationally important wetland registered in the ramsar convention. the metal contents in the mussel species from the studied region were comparable to other world areas. in most cases, the levels of the metals either fell within the range for other areas or were lower. there were signific ... | 2010 | 19266295 |
| [the effect of pesticide acetamiprid on biochemical markers in tissues of fresh water bivalve mussels anodonta cygnea l. (unionidae)]. | the effects of ecological real concentration of pesticide "mospilan" (active substance - acetamiprid) on the status of oxidative stress biomarkers, neurotoxicity and metallothioneins' (mts) characteristics of freshwater bivalve mussels anodonta cygnea were investigated. the prooxidative changes (decrease of superoxide dismutase activity, reduced glutathione content, increase of content of lipid and proteins peroxidation products) and decrease of the mts content in gills and especially in digesti ... | 2016 | 19248625 |
| seasonal variations of ph, pco2, po2, hco3- and ca2+ in the haemolymph: implications on the calcification physiology in anodonta cygnea. | a study about the relationship between the physical-chemical parameters and the calcium carbonate balance between the haemolymph fluid and mantle calcareous structures was carried out in anodonta cygnea. an intense peak of hco(3) (-) and a highest ph in december-january months may be understood as a preparation period for creating alkaline conditions. an intense ph decrease from january to february in parallel with the hco(3) (-) reduction seems to indicate the beginning process of carbonate pre ... | 2009 | 18985356 |
| multi-biomarkers approach in different organs of anodonta cygnea from the dnister basin (ukraine). | the aim of this study was to compare environmental quality in two sites in western ukraine-rural (r) and urbanized (u)-with the usage of the resident bivalve mollusk anodonta cygnea. the study was realized during three seasons. the metal uptake and a set of biochemical markers were determined. for each season, cd and metallothioneins (mts) contents in the digestive gland and gills of the mollusc were higher at the u site, reflecting its chronic pollution. the oxidative stress in the mollusk was ... | 2009 | 18841409 |
| a mathematical model of the proton balance in the outer mantle epithelium of anodonta cygnea l. | in the freshwater mollusc anodonta cygnea and other unionids, the mantle plays an important role in the regulation of the movements of ions between the shell and the extrapaleal fluid. in this report, a mathematical model that attempts to describe the cell metabolic mechanisms underlying the operation of the outer mantle epithelium as a source of protons is presented. we encoded the information gathered by studying the epithelium in vitro, which includes the electrophysiology of the preparation, ... | 2008 | 18587603 |
| natural waste materials containing chitin as adsorbents for textile dyestuffs: batch and continuous studies. | in this work three natural waste materials containing chitin were used as adsorbents for textile dyestuffs, namely the anodonta (anodonta cygnea) shell, the sepia (sepia officinalis) and the squid (loligo vulgaris) pens. the selected dyestuffs were the cibacron green t3g-e (ci reactive green 12), and the solophenyl green ble 155% (ci direct green 26), both from ciba, commonly used in cellulosic fibres dyeing, the most used fibres in the textile industry. batch equilibrium studies showed that the ... | 2005 | 16140355 |
| isolation, purification and characterization of glycosaminoglycans in the fluids of the mollusc anodonta cygnea. | the isolation, purification and characterization of anodonta cygnea haemolymph, and extrapallial fluid glycosaminoglycans (gags), which have high calcium affinity, were carried out in order to better understand the process of nacreous shell biomineralization. our results show the existence of two different gags with similar contents in the two fluids, throughout the year, but with significant seasonal variations for both. in the winter months, we identified by electrophoresis, only one kind of g ... | 2005 | 16006160 |
| molecular mechanisms of the regulatory effect of biogenic amines on the functional activity of the adenylate cyclase signal system in nerve ganglia of the mollusk anodonta cygnea. | | 2015 | 15999832 |
| sensitivity of adenylyl cyclase signaling system of the mollusk anodonta cygnea ganglions to serotonin and adrenergic agonists. | for the first time, the adenylyl cyclase system (acs) sensitive to biogenic amines in the mollusk anodonta cygnea ganglions was revealed and characterized. serotonin and isoproterenol stimulated ac activity and gtp-binding activity of heterotrimeric g-proteins. the ac-stimulating action of serotonin and isoproterenol was blocked by cyproheptadine and adrenergic antagonists, respectively. using synthetic c-terminal peptides of g-protein alpha-subunit, it was shown that the biogenic amines realize ... | 2005 | 15891091 |
| pleiotropic action of insulin-like peptides of mollusk, anodonta cygnea. | insulin-related peptides (irps) from ganglions of mollusk, anodonta cygnea, were purified and characterized (irp1-irp13) using insulin and insulin-like growth factor-i (igf-i) radioreceptor test systems. the irps were able to bind to insulin and igf-i receptors. dose-dependent curve slopes indicated that most irps bind with higher affinity to igf-i receptors than to insulin receptors. the irp regulatory action on the activity of the adenylyl cyclase signal system showed that these peptides stimu ... | 2005 | 15891090 |
| the concentration-specific response of metallothioneins in copper-loading freshwater bivalve anodonta cygnea. | metallothioneins (mts) from digestive gland of freshwater bivalve mollusc anodonta cygnea, exposed to 10 or to 200 microg x l(-1) copper ions during 14 days, were resolved using subsequent gel-permeation and ion-exchange chromatography on mt-1 and mt-2. in both groups of treated molluscs the content of copper in mt-2 was elevated twice. the significant elevation of this isoform, which is minor in control animals, and the increasing of the zinc level in it were also observed after treatment with ... | 2005 | 19618744 |
| h+-atpase of crude homogenate of the outer mantle epithelium of anodonta cygnea. | the outer mantle epithelium of the freshwater bivalve, anodonta cygnea, is responsible for the mineralisation of the shell. under short circuit conditions, it generates a current that is due to the operation of an electrogenic proton pump located in the apical barrier of that epithelium. bafilomycin a1 and concanamycin a inhibited the short circuit current. the ic50 and maximum inhibition dose were 0.17 and 0.5 microm for bafilomycin a1, and 0.7 and 5 microm for concanamycin a. the present work ... | 2004 | 15596387 |
| paracellular pathway in the shell epithelium of anodonta cygnea. | ultrastructural study of cell-cell connections in the outer mantle epithelium (ome) on high-pressure-frozen specimens revealed zonula adherens, septate junctions and gap junctions in anodonta cygnea. in order to evaluate the permeability of the paracellular pathway, the ome was incubated under gradients of lanthanum and calcium. after lanthanum incubation (4 mm) from the basal side, the septate junctions were penetrated completely by this tracer. when applied from the apical side, lanthanum depo ... | 2004 | 15114649 |
| [insulin-like peptides of cerebro-pleural ganglia of the mollusc anodonta cygnea: isolation, purification and radioligand analysis]. | | 2011 | 14520897 |
| [insulin-like growth factor-1 receptor in tissues of the mollusk anodonta cygnea ]. | | 2011 | 12815967 |
| effect of sub-lethal concentrations of aluminium on the filtration activity of the freshwater mussel anodonta cygnea l. at neutral ph. | significant amounts of aluminium (al) are commonly present in rivers and lakes, largely in particulate form in neutral waters. freshwater bivalves, as filter feeders are therefore exposed to both particulate and dissolved metal and are potentially vulnerable to al. the effect of al on filtering behaviour of the freshwater mussel anodonta cygnea l. was investigated during short (1 hour) and long-term (15 days) exposure to environmentally relevant concentrations (250 and 500 microg l(-1)) at neutr ... | 2002 | 12501933 |
| cytometric, morphologic and enzymatic characterisation of haemocytes in anodonta cygnea. | the haemocytes in bivalve mussels are involved in many processes such as lesion repair, shell repair, elimination of small particles and toxic substances. in anodonta cygnea there are two categories of haemolymph cells, the granulocytes and hyalinocytes. two groups of cells were identified by flow cytometry and morphological studies: one with larger size and granularity representing 75%, and another group of cells (25%) which were approximately half the size. the cytochemical reactions showed pe ... | 2002 | 12044764 |
| morphological and chemical characterization of mineral concretions in the freshwater bivalve anodonta cygnea (unionidae). | the freshwater mussel anodonta cygnea is commonly used as a model organism for biomineralization studies, its peculiar morphofunctional properties also make it an excellent environmental biomonitor. the first detailed on the calcareous concretions from gill and mantle tissue, as well as fluids of the freshwater bivalve a. cygnea, supported by histological, scanning, spectrometry, and spectroscopy analyses. through these analyses, the morphology, structure, and chemical characterization of these ... | 2015 | 25242687 |
| the importance of the mining subsidence reservoirs located along the trans-regional highway in the conservation of the biodiversity of freshwater molluscs in industrial areas (upper silesia, poland). | the objectives of the survey were to analyse the structure of the mollusc communities in the mining subsidence reservoirs that were created as a result of land subsidence over exploited hard coal seams and to determine the most predictive environmental factors that influence the distribution of mollusc species. the reservoirs are located in urbanised and industrialised areas along the trans-regional highway, which has a high volume of vehicular traffic. they all have the same sources of supply b ... | 2017 | 26005225 |
| the swan mussel (anodonta cygnea) in anzali wetland of iran, a potential biomonitor for cd and pb. | protecting wetlands from environmental pollution has been of significant importance, as they are the vital habitats for various kinds of birds and animals. this study has aimed to monitor the contamination of cd and pb in anzali wetland, located in the north of iran using a mussel biomonitor. the contents of cd and pb were measured in the surface sediment, the soft tissue, and the shell of a. cygnea. the samples were collected from four sites in the region. the results demonstrated that the shel ... | 2014 | 24934707 |
| interspecies differences in p-glycoprotein mediated activity of multixenobiotic resistance mechanism in several marine and freshwater invertebrates. | the presence and function of the p-glycoprotein mediated multixenobiotic resistance (mxr) mechanism was demonstrated in numerous aquatic organisms. the aim of this study was to investigate whether in aquatic organisms exists the inherent, species-specific basal level of mxr activity. here the results of the direct comparison of the basal (noninduced) level of mxr activity measured in several marine (mytilus galloprovincialis, monodonta turbinata, patella lusitanica) and freshwater (dreissena pol ... | 2000 | 11050689 |
| organic compounds in the extrapalial fluid and haemolymph of anodonta cygnea (l.) with emphasis on the seasonal biomineralization process. | bivalve mollusks, such as the freshwater mussel anodonta cygnea, show seasonal changes in calcification. this cycle of calcification must either be a cause or a consequence for seasonal fluctuations in the organic composition of the animal's fluids, haemolymph and extrapallial fluid, the liquid media for biomineralization. we monitored the fluids of a. cygnea, throughout a 1-year cycle, for the presence of organic constituents, known to be important for biomineralization, such as proteins, glyco ... | 2000 | 10818264 |
| the action of cd, cu, cr, zn, and pb on fluid composition of anodonta cygnea (l.): organic components. | the heavy metals, cd, cu, cr, zn, and pb, were used to incubate healthy specimens of the freshwater mussel species, anodonta cygnea. afterwards, their biological fluids, either haemolymph or extrapallial fluid were analyzed for the presence of several organic constituents, known to be important for biomineralization, such as proteins, glycosaminoglycans (gags) and glucosamine. proteins were subjected to further study, namely through the total amino acid determination after acid hydrolysis. the m ... | 2000 | 11126745 |
| [the role of sulfhydryl groups in functioning of components of adenylate cyclase signal system in smooth muscles of the mollusk anodonta cygnea (effect of n-ethylmaleimide and p-chloromercuribenzoic acid)]. | the alkylating agent n-ethylameimide and the sulfhydryl group blocker p-chloromercuribenzoic acid (cpma) inhibited in dose-dependent manner both basal activity of adenylyl cyclase (ac) and its activity stimulated by non-hormonal substances (forskolin, sodium fluoride, guanylilimidodiphosphate) in smooth muscles of the freshwater bivalve mollusk anodonta cygnea. the double increase (from 30 to 60 min) in the time of preincubation of a sarcolemmal membrane fraction with ethylmaleimide and cpma led ... | 1999 | 10505332 |
| short- and long-term effects of deltamethrin on filtering activity of freshwater mussel (anodonta cygnea l.). | bioindicator methods were used to study the effects of the pyrethroid insecticide deltamethrin on the filtering behavior of the freshwater mussel anodonta cygnea l in the laboratory, during short-term (30 min) and long-term (1 week) exposure. in the course of the short-term treatment, the water flow through the outflow syphon was monitored. it was found that 1 and 5 micrograms/liter deltamethrin caused an increase, whereas from 10 to 50 micrograms/liter deltamethrin resulted in a depression, in ... | 1997 | 9469868 |
| the effects of pesticides on monoaminergic system related to periodic activity of mussels (anodonta cygnea l.). | 1. the effect of a herbicide (paraquat) and an insecticide (methidation) on the periodic activity of mussel in relation to serotonin and dopamine levels and their release and uptake in nervous system was investigated. 2. lc50 values of methidation in 24-, 48-, and 96-hr treatments, respectively, were 50% lower than those of paraquat. 3. serotonin and dopamine levels decreased in all three ganglia by 20% on average upon 24- and 48-hr in vivo treatments with paraquat. in the 96-hr treatment, howev ... | 1997 | 9195197 |
| accumulation of extractable organic halogens (eox) by the freshwater mussel, anodonta cygnea l., exposed to chlorine bleached pulp and paper mill effluents. | freshwater mussels, anodonta cygnea l., were collected in a lowly polluted area in north-eastern france, and exposed during 28 days at three sites up and downstream of a chlorine bleached pulp and paper mill effluent water, sediment and mussel samples were collected at regular intervals and analyzed for halogenated organic residues using the sum parameters aox (adsorbable organic halogen) and eox (extractable organic halogen). the survey of the water showed a chronic contamination by organic hal ... | 1996 | 8867150 |
| tachykinin- and leucokinin-related peptides in the molluscan nervous system. | distribution of locustatachykinin-like immunoreactive (lomtkli) and leucokinin like immunoreactive (lkli) neurons in the central nervous system (cns) of the pond snail, lymnaea stagnalis was investigated and compared to that found in helix pomatia. occurrence of lomtkli neurons in different ganglia of the freshwater bivalve, anodonta cygnea, was also studied. similar to helix, the lymnaea cns contained lomtkli and lkli neurons mainly in the cerebral and pedal ganglia, but the number of labelled ... | 1995 | 8853699 |
| [the role of a cosubstrate in the kinetic mechanism of action of mollusk thiaminase i]. | mechanisms of interaction between thiaminase i and cosubstrate were studied using thiazole-2-14c-thiamine and 14c-nicotinic acid. the dissociation constant of nicotinic acid in the presence of the enzyme was of an order of 1 x 10(-6) m. a mechanism of the prestationary phase of a thiaminase reaction at -10 degrees was studied. the low temperature step, which demonstrated specific characteristics, was involved in analytic estimation of total thiamine concentration in vitro. native thiaminase i fr ... | 2015 | 8511878 |
| acetylcholine level in the brain and other organs of the bivalve anodonta cygnea l. and its modification by heavy metals. | acetylcholine was detected and measured in the ganglia (60-80 nmol/g), in the heart (10-15 nmol/g) and in the adductor muscles (4-5 nmol/g) of the bivalve anodonta cygnea l. using gas chromatographic determination. treatment of the animals with low concentration of heavy metals, which cause change in the behaviour, resulted in decrease of the brain acetylcholine level. within 7 days treatment cu2+ caused 80 per cent, cd2+ and pb2+ 30 per cent reduction with varying types of recovery after wash. | 1993 | 8493846 |
| beta-agonist-induced inhibitory-guanine-nucleotide-binding regulatory protein coupling to adenylate cyclase in mollusc anodonta cygnea foot muscle sarcolemma. | in the sarcolemma fraction of foot muscles of a fresh-water bivalve mollusc, anodonta cygnea, a direct inhibitory, rather than stimulatory, effect of the beta-adrenergic agonist isoproterenol, at micromolar concentration, on camp level and adenylate cyclase activity, was revealed. it was blocked by beta- but not alpha-adrenergic antagonists. a single class of [3h]dihydroalprenolol-binding sites with binding properties of beta-adrenergic receptor was detected in mollusc sarcolemma. potentiation o ... | 1992 | 1332863 |
| cadmium kinetics in freshwater clams. v. cadmium-copper interaction in metal accumulation by anodonta cygnea and characterization of the metal-binding protein. | freshwater clams were exposed for 6 1/2 weeks to cadmium chloride, at 48 ppb1 cd, or to copper chloride, at 47 ppb cu, or to a mixture of the salts, at 122 ppb cd + 139 ppb cu. the cd-accumulation factor (af) for whole animal was reduced by 90% in the presence of cu. cu-af was reduced by 50% in the presence of cd. copper did not influence the in vitro uptake of cd by the excised gill but increased the elimination of previously accumulated cd. however, decrease of the ventilation rate probably ac ... | 1991 | 1953031 |
| [a new mechanism of inhibiting adenylate cyclase involving a beta-adrenergic receptor]. | the previously unknown mechanism of adenylate cyclase activity inhibition by catecholamines has been found. it is realized through a beta-adrenoreceptor in the smooth muscle of fresh-water mollusc anodonta cygnea. as to its ligand-binding characteristics (one class of binding sites with kd = 0.35 + 0.06 nm, a competitive series of ligands substitution: isoproterenol greater than adrenalin greater than propranolol greater than noradrenaline greater than serotonin = dopamine greater than phentolam ... | 2015 | 1656572 |
| how is o2 consumption maintained independent of ambient oxygen in mussel anodonta cygnea? | the mechanisms of adaptation allowing resting freshwater mussels anodonta cygnea to maintain their oxygen consumption (mo2) constant when the o2 partial pressure in the inspired water (pio2) varied were studied at 13 degrees c. steady-state values of oxygen consumption and/or shell valve activity were determined at prefixed pio2 for periods ranging from 2 to 15 days. values of po2, o2 concentration and acid-base status of arterial blood in the heart were determined after two days. mo2 was mainta ... | 1991 | 2028102 |
| effects of cadmium in freshwater clams. iii. interaction with energy metabolism inanodonta cygnea. | freshwater clams,anodonta cygnea, were exposed to cadmium, at 50 ppb (μg/l), to investigate the effects of semi-chronic exposure on energy metabolism. parameters examined included: adenylate energy charge (aec), glycogen content, blood glucose and protein concentration, the accumulation of anaerobic metabolic end products,viz. lactate and succinate, and mitochondrial nadh-oxidase activity. in all tissues, aec was significantly lowered after 12 weeks. glycogen contents of the separate organs, exc ... | 2011 | 21318501 |
| effects of cadmium in freshwater clams. ii. ultrastructural changes in the renal system ofanodonta cygnea. | an ultrastructural study was made of the renal system of freshwater clams,anodonta cygnea, that had been exposed to cadmium chloride (50 μg cd/l) for 12 weeks. by stereological analysis an extended lysosomal system and a decreased number of mitochondria was apparent in the epithelial cells lining the proximal compartment of the kidney. the increase of the lysosomal system was mainly accountable to the appearance of a distinct type of lysosome, that accumulated in the apical cell region. the decr ... | 2011 | 21318500 |
| effects of cadmium in freshwater clams. i. interaction with essential elements inanodonta cygnea. | the elemental composition of the freshwater clam,anodonta cygnea, was investigated in response to exposure to cadmium at 50 ppb (μg/l) cd during 12 weeks. accumulation of cadmium affected the composition in various tissues and hemolymph. from 2 to 8 weeks of exposure considerable loss of sodium occurred, to about half the levels in control animals. during the last four weeks of exposure, sodium concentrations stabilized at the decreased level while those of potassium started to decline. minor ch ... | 2011 | 21318499 |
| [beta-adrenoreceptors in the muscular tissue of anodonta cygnea]. | the beta-adrenoceptors in the muscular tissue of anodonta cygnea have been studied for the first time with the use of antagonist [125i] iodocyanopindolol. the tissue membrane had only one class of binding sites with kd 2.9 +/- 0.02 pm and the maximal number of binding sites (bmax) 110 +/- 2.4 fmoles/mg of protein. the potency of beta-agonists and antagonists for displacing [125i] iodocyanopindolol for its beta-ar complexes was the following: isoproterenol greater than adrenaline greater than nor ... | 2015 | 2561031 |
| distinct glutathione-dependent enzyme activities and a verapamil-sensitive binding of xenobiotics in a fresh-water mussel anodonta cygnea. | a fresh-water mussel anodonta cygnea, an aquatic invertebrate resistant to pollution, possesses an inherent high potential to bind 2-acetylaminofluorene onto membrane vesicles. this binding is saturable and trypsin- and verapamil-sensitive. simultaneously, this mussel reveals a relatively high inherent activity of glutathione-dependent enzyme activities with a distinct spectrum of substrate affinities. both these activities are similar to the elements of the molecular mechanism involved in the a ... | 1989 | 2818596 |
| cadmium kinetics in freshwater clams. iv. histochemical localization of cadmium in anodonta cygnea and anodonta anatina, exposed to cadmium chloride. | | 1988 | 3377537 |
| ultrastructural and cytochemical studies in the mantle of anodonta cygnea. | the large central region of the mantle of anodonta cygnea was studied using transmission and scanning electron microscopy (tem and sem) for ultrastructural analysis and light microscopy (lm) and tem for cytochemical analysis. x-ray diffraction studies of the organic matrix pellicle deposited on the inner surface of the shell were also carried out. two groups of columnar cells presenting desmosomes on the apical region were observed in the outer epithelium. one group secretes a structural and neu ... | 1988 | 18620246 |
| cadmium kinetics in freshwater clams. iii. effects of zinc on uptake and distribution of cadmium in anodonta cygnea. | | 1987 | 3813660 |
| effect of mosquito killer insecticides on freshwater mussels. | the effect of four insecticides (fyfanon, k-othrin, unitox 7 and unitox 20) was investigated on the freshwater mussels anodonta cygnea l., anodonta anatina l. and unio pictorum l. the studies were performed in hard water (total salinity of 310 mg/l). the lc50 values for 24, 48, 72, 96 hr and 7 days were determined with a static test at 22 degrees c. all the three mussel species proved to be extremely tolerant against the insecticides tested. the effect of sublethal concentrations of these insect ... | 1987 | 2881711 |