| direct detection and identification of enteroviruses from faeces of healthy nigerian children using a cell-culture independent rt-seminested pcr assay. | recently, a cell-culture independent protocol for detection of enteroviruses from clinical specimen was recommended by the who for surveillance alongside the previously established protocols. here, we investigated whether this new protocol will show the same enterovirus diversity landscape as the established cell-culture dependent protocols. faecal samples were collected from sixty apparently healthy children in ibadan, nigeria. samples were resuspended in phosphate buffered saline, rna was extr ... | 2016 | 27087810 |
| molecular typing and characterization of a new serotype of human enterovirus (ev-b111) identified in china. | molecular methods, based on sequencing the region encoding the complete vp1 or p1 protein, have enabled the rapid identification of new enterovirus serotypes. in the present study, the complete genome of a newly discovered enterovirus serotype, strain q0011/xz/chn/2000 (hereafter referred to as q0011), was sequenced and analyzed. the virus, isolated from a stool sample from a patient with acute flaccid paralysis in the tibet region of china in 2000, was characterized by amplicon sequencing and c ... | 2014 | 24503225 |