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characterization and complete nucleotide sequence of strawberry mottle virus: a tentative member of a new family of bipartite plant picorna-like viruses.an isolate of strawberry mottle virus (smov) was transferred from fragaria vesca to nicotiana occidentalis and chenopodium quinoa by mechanical inoculation. electron micrographs of infected tissues showed the presence of isometric particles of approximately 28 nm in diameter. smov-associated tubular structures were also conspicuous, particularly in the plasmodesmata of c. quinoa. dsrna extraction of smov-infected n. occidentalis yielded two bands of 6.3 and 7.8 kbp which were cloned and sequence ...200211752720
a new potato virus in a new lineage of picorna-like viruses.on constructing a cdna library for potato, 'contaminating' sequences with a significant identity to apple latent spherical virus (alsv) were found. determination of the remaining genome sequence indicated the presence of a bipartite virus with an rna1 and 2 of 7034 and 3315 nucleotides, respectively, excluding a poly(a)tail. rna1 encodes a single polyprotein (233 kda) and shares highest amino acid identity with alsv at 65%. conserved amino acid motifs typical for helicase, protease and rna-depen ...200415503203
cheravirus and sadwavirus: two unassigned genera of plant positive-sense single-stranded rna viruses formerly considered atypical members of the genus nepovirus (family comoviridae).the genus nepovirus (family comoviridae) was known both for a good level of homogeneity and for the presence of atypical members. in particular, the atypical members of the genus differed by the number of capsid protein (cp) subunits. while typical nepoviruses have a single cp subunit with three structural domains, atypical nepoviruses have either three small cp subunits, probably corresponding to the three individual domains, or a large and a small subunit, probably containing two and one struc ...200717585366
a new virus related to satsuma dwarf virus: the nucleotide sequence of the 3'-terminal regions of hyuganatsu virus rnas 1 and 2. brief report.the sequences of the 3'-terminal 1306 and 2160 nucleotides of rnas 1 and 2 of a virus serologically related to satsuma dwarf virus (sdv) from hyuganatsu ( citrus tamurana hort. ex tan.) were determined, respectively. we found that the partial rna-dependent rna polymerase region in rna1 and the coat proteins (cps) region in rna2 of the virus tentatively named hyuganatsu virus (hv) have 78.3-84.0% and 76.9-80.7% amino acid sequence identities to those of known sdv-related viruses (sdv-rvs), i.e., ...200415221545
satsuma dwarf and related viruses belong to a new lineage of plant picorna-like viruses.satsuma dwarf virus (sdv) and two closely related viruses, citrus mosaic (cimv), and naval orange infectious mottling (nimv), seriously affect citrus varieties grown in japan and east asia. all three viruses have icosahedral particles built of two proteins encapsidating two single-stranded genomic rnas. the natural mode of transmission of these sdv-like viruses is unknown, and they were previously placed among tentative members of the family comoviridae. recently, a complete genome of sdv was se ...200111556400
sequence diversity and interrelationships among isolates of satsuma dwarf-related viruses.the 3'-region of rna2 of three viruses (natsudaidai dwarf virus (isolate nd-1), and two unidentified isolates (lb-1, az-1)), which were related to satsuma dwarf virus (sdv), were sequenced. phylogenetic analysis including the previously reported sdv-related viruses (citrus mosaic virus (cimv, ci-968), navel orange infectious mottling virus (nimv, ni-1)) showed that they were classified into three groups, sdv (s-58), cimv (ci-968, lb-1, az-1, nd-1), and nimv (ni-1). the results suggested these gr ...200111402866
the nucleotide sequence of the coat protein genes of satsuma dwarf virus and naval orange infectious mottling virus.the sequence of the 3'-terminal 4320 and 2409 nucleotides were determined for rna2 of satsuma dwarf virus (sdv) and navel infectious mottling virus (nimv). both sequences contained a part of a long open reading frame which encodes larger and smaller coat proteins (cps) at the 3'-terminus followed by a 3'non-coding region upstream of a poly (a) tail. amino acid sequence identity for larger and smaller cps ranged 81-84% and 68-78%, respectively, among sdv, nimv and the previously sequenced citrus ...19989541624
nucleotide sequences and taxonomy of satsuma dwarf virus.the nucleotide sequences of genomic rna1 (6795 nt) and rna2 (5345 nt) of satsuma dwarf virus (sdv), a tentative member of the genus nepovirus, were determined. the deduced genome organization of sdv showed similarities to the organization in como-, faba- and nepoviruses. there is extensive amino acid sequence similarity in the n-terminal regions of the proteins encoded by rna1 and rna2, as reported previously only for tomato ringspot nepovirus. however, unlike definitive nepoviruses, which have ...199910092021
development of multiplex pcr for simultaneous detection of citrus viruses and the incidence of citrus viral diseases in late-maturity citrus trees in jeju island.satsuma dwarf virus (sdv) or citrus mosaic sadwavirus (cimv) were not consistently detected in rt-pcr assay with the primer sets based on gene of japan isolates. sdv and cimv isolates were distinctively divided into two groups based on phylogenetic analysis of pp2 gene cloned from 22 korean isolates, and the korean cimv and sdv isolates shared 95.5-96.2% and 97.1-97.7% sequence identity with japanese isolate, respectively. we developed pp2-1 primer set based on the pp2 gene sequence of korean is ...201728592949
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