| rickettsiae-like microorganisms in the midgut and other visceral tissues during development of drosophila auraria. | rickettsiae-like organisms (rlos) were identified for the first time in midgut, malpighian tubules, wreath cells (ventral nephrocytes), spermatogonia and gut muscles in a species of drosophila. their number in the midgut cells of drosophila auraria significantly increased at the late third larval instar and at the beginning of pupation. the rlo population in the larval midgut followed the fate of their host cells and was destroyed during metamorphosis. the rlos pass from the larval to the adult ... | 1992 | 1394092 |
| rickettsiae-like structures in the larval salivary gland cells of drosophila auraria. | rickettsiae-like structures were found in the salivary gland cells of drosophila auraria during different larval and prepupal developmental stages, from the early 3rd instar up to 14 hr after spiracle inversion. these microorganisms are surrounded by a membrane, are constantly intracellular, and occur singly or in groups. their widespread occurrence in various tissues of other drosophila species indicates that they can be considered as symbionts, but their actual functional significance (if any) ... | 1991 | 1999840 |
| latitudinal clines in the properties of a circadian pacemaker. | the circadian rhythm of eclosion activity and its pacemaker were analyzed in a series of latitudinal races of drosophila auraria ranging from 34.2 degrees to 42.9 degrees n in japan. the phase of the rhythm (psi el) to the daily photoperiod (pp) changes as daylength is increased, and the slope of psi el (pp) changes with latitude. is is sufficiently greater in the north to cause a phase reversal of northern and southern races on long versus short photoperiods. this reversal is found in assays of ... | 1989 | 2519590 |
| the organization of the alpha-tubulin gene family in the drosophila montium subgroup of the melanogaster species group. | the alpha 1-, alpha 2-, alpha 3-, and alpha 4-tubulin genes have been mapped by in situ hybridization to the polytene chromosomes of five species representative of the drosophila montium subgroup geographical distribution. a lambda phage clone containing alpha 1-tubulin specific sequences was isolated from a genomic dna library of drosophila auraria and its restriction endonuclease pattern is presented. both well-characterized heterologous and homologous probes were used to assess orthogonality ... | 1998 | 9796099 |
| molecular phylogeny of the drosophila auraria species complex and allied species of japan based on nuclear and mitochondrial dna sequences. | we studied the phylogenetic relationships among the six species of the drosophila auraria species complex and three species of the montium species subgroup endemic to japan. to do this, we examined the dna sequences of the nuclear its1 and the mitochondrial coii of these species. intraspecific variation in its1 was observed for most of the species studied. however, interspecific variation was small and of the same magnitude as intraspecific variation among d. auraria, d. triauraria, and d. quadr ... | 2007 | 17396022 |
| comparison of the polytene chromosomes of the salivary gland, the fat body and the midgut nuclei of drosophila auraria. | photo-maps of the fat body and midgut polytene chromosomes of drosophila auraria were constructed. these photo-maps are compared with a new, more detailed photo-map of the salivary gland chromosomes of the same species. seven, not previously described inverted tandem-duplications were detected, raising the number of such structures found in this species to 31. the constancy of the banding pattern based on the analysis of the above chromosomes is discussed. | 2008 | 2262142 |
| the hsp70 locus of drosophila auraria (montium subgroup) is single and contains copies in a conserved arrangement. | the restriction endonuclease pattern of a number of hsp70-homologous clones isolated from a library of heat shock cdna from drosophila auraria, a species belonging to the montium subgroup of the melanogaster species group, reveals two types of clones, a and b, differing in a single restriction site. both types, as well as hsp70-specific probes derived from both hsp70 loci of drosophila melanogaster, hybridize in situ with a single band at region 32 a of the 2l polytene arm, indicating a clustere ... | 1998 | 9933411 |
| what the loss of the hormone neuroparsin in the melanogaster subgroup of drosophila can tell us about its function. | the twelve sequenced drosophila genomes show the vast majority of neurohormone and neuropeptide genes to be very well conserved. nonetheless, the gene encoding the hormone neuroparsin has undergone significant evolution. although drosophila melanogaster has one of the best known genomes, no neuroparsin gene can be detected in either the assembled genome or any of the individual sequencing traces. this gene is similarly absent from the genomes of other species in the melanogaster subgroup, even t ... | 2010 | 20226240 |
| genetic changes to a transcriptional silencer element confers phenotypic diversity within and between drosophila species. | the modification of transcriptional regulation has become increasingly appreciated as a major contributor to morphological evolution. however, the role of negative-acting control elements (e.g. silencers) in generating morphological diversity has been generally overlooked relative to positive-acting "enhancer" elements. the highly variable body coloration patterns among drosophilid insects represents a powerful model system in which the molecular alterations that underlie phenotypic diversity ca ... | 2015 | 26115430 |
| isolation, characterization, and localization of beta-tubulin genomic clones of three drosophila montium subgroup species. | genomic libraries were constructed from three drosophila species, namely drosophila auraria, drosophila serrata, and drosophila kikkawai, belonging to the drosophila montium subgroup of the drosophila melanogaster species group. clones containing beta-tubulin specific sequences were isolated, characterized by restriction endonuclease digestions and southern hybridizations, and mapped by in situ hybridization on the polytene chromosomes of the species studied. the distribution of the beta-tubulin ... | 2002 | 12033631 |
| [molecular phylogeny of drosophila auraria species complex]. | drosophila auraria species complex consists of five sibling species d. auraria, d. biauraria, d. triauraria, d. quadraria and d. subauraria. the complete sequences of nuclear its1 (internal transcribed specer 1) and mitochondrial co ii (cytochrome oxdase ii), and partial sequences of nuclear adh (alcohol dehydrogenase) of these five sibling species and their closely-related species, d. rufa, were determined. using d. rufa, d. melanogaster and d. yukuba as outgroups, both most-parsimony (mp) and ... | 2002 | 11838374 |
| ultrastructural studies on the effect of heat shock treatment on larval salivary gland cells of drosophila auraria. | in this study, the effect of heat shock treatment on drosophila auraria late 3rd instar larval salivary glands was examined. heat shock treatment was applied on whole animals and on isolated salivary glands. the fine structural changes were examined using transmission electron microscopy, after a temperature rise from normal (25 +/- 1degreesc) to 37 degreesc or 40 degreesc for various periods of time. the acpace histochemical technique was used to demonstrate the acid phosphatase activity on lys ... | 2000 | 11297376 |
| mass determination of the unit cell of the innermost chorionic layer in drosophilidae by scanning transmission electron microscopy. | the innermost chorionic layer (icl) in eggshells of drosophila melanogaster is a naturally occurring patchwork of thin three-dimensional crystalline plates located between the inner endochorion and the vitelline envelope. the mass-per-unit area of the icl has been measured from scanning transmission electron microscope images of isolated unstained material and it was possible to distinguish up to four layers with the majority of the crystalline sheets being one to three layers thick. taking into ... | 1999 | 10544051 |
| the heat-shock gene hsp83 of drosophila auraria: genomic organization, nucleotide sequence, and long antiparallel coupled orfs (lac orfs). | the genomic organization of the hsp83 gene of drosophila auraria, a far-eastern endemic species belonging to the montium subgroup of the melanogaster species group, is presented here. based on in situ hybridization on polytene chromosomes, cdna and genomic clone mapping, nucleotide sequencing, and genomic southern analysis, hsp83 is shown to be present as a single-copy gene at locus 64b on the 3l chromosome arm in d. auraria. this gene is organized into two exons separated by a 929-bp intron. th ... | 1998 | 9493358 |
| heat shock gene expression during recovery after transient cold shock in drosophila auraria (diptera: drosophilidae). | newly synthesized polypeptides during recovery from prolonged cold treatment (0 degree to -1 degree c) of drosophila auraria, a montium subgroup species, of the melanogaster species group, were analysed in denaturing polyacrylamide gels. in addition, during the cold shock recovery period, northern analysis of the hsp83 mrna was performed. a significant induction of two polypeptides, which exhibited electrophoretic mobilities, with the heat inducible 83 and 70 kd hsp83 and hsp70 was detected, but ... | 1997 | 9693879 |
| a drosophila hsp70 gene contains long, antiparallel, coupled open reading frames (lac orfs) conserved in homologous loci. | a clone isolated from a drosophila auraria heat-shock cdna library presents two long, antiparallel, coupled (lac) open reading frames (orfs). one strand orf is 1,929 nucleotides long and exhibits great identity (87.5% at the nucleotide level and 94% at the amino acid level) with the hsp70 gene copies of d. melanogaster, while the second strand orf, in antiparallel in-frame register arrangement, is 1,839 nucleotides long and exhibits 32% identity with a putative, recently identified, nad(+)-depen ... | 1995 | 7563128 |
| genetic basis and evolution of species-specific courtship song in the drosophila auraria complex. | the interpulse interval (ipi) of courtship song in the drosophila auraria complex is the only parameter that is consistently species-specific among the several courtship elements examined within the complex. the genetic basis of the species-specific courtship song was examined by analysing the song of interspecific hybrids and of backcross progeny. ipi of all interspecific hybrids except two showed intermediate values, suggesting autosomal control of species-specific ipi. however, significant de ... | 1994 | 8206363 |
| the beta-tubulin genes of drosophila auraria are arranged in a cluster. | when the beta 1-, beta 2- and beta 3-tubulin-specific dnas from drosophila melanogaster were used as probes to recognize tubulin-specific sequences in the chromosomes of drosophila auraria, they were found to hybridize to the same polytene band in region 32c of the 2l polytene chromosome. three overlapping clones were isolated from a lambda embl3 genomic library of d. auraria, and they all contain beta-tubulin-specific sequences based on hybridization and partial-sequencing experiments of subclo ... | 1994 | 8082170 |
| [study on the genetics of drosophila auraria species complex]. | | 1994 | 7848665 |
| [study on evolutionary genetics of drosophila auraria species complex--cladistic analysis and phenetic analysis]. | in the present paper, seventeen geographic strains of d. auraria species complex and five geographic strains of other four species, which belong to the montium species subgroup were biochemically examined for the construction of phylogenetic tree based on phenetic and cladistic analysis. eighteen isoenzymes were analyzed by isoelectric focusing polyacrylamide gel electrophoresis in the above twenty two geographic strains. the phenogram of the d. auraria species complex is supported by upgma prog ... | 1994 | 7718276 |
| insecticidal effects of essential oils. a study of the effects of essential oils extracted from eleven greek aromatic plants on drosophila auraria. | effects of the essential oils (eos) extracted from eleven aromatic plants belonging to the lamiaceae family (common in the greek flora) were examined upon three different developmental stages of drosophila auraria. all of the eos examined exhibited insecticidal effects, either by preventing egg hatching, or by causing the death of larvae and adult flies. in several cases, malformation and/or prohibition of puparium formation was also observed. | 1992 | 1612141 |
| functional morphology of the crop of drosophila auraria. | the crop epithelium of drosophila auraria consists of a single layer of thin epithelial cells whose apex is covered with cuticle. the epithelial cells contain small amounts of rough endoplasmic reticulum, golgi complexes and secretory granules. the digestive activities of the crop cavity are probably due to enzymes provided by the salivary glands and midgut cells. the epithelium displays continuous folds, which are more frequent in the upper region. the crop of drosophila is capable of expanding ... | 1992 | 1505206 |
| complex carbohydrate presence in the gut and malpighian tubules of drosophila auraria larvae (insecta, diptera): a cytochemical study. | cytochemical techniques were used for the qualitative characterization of carbohydrates in the foregut, midgut, hindgut and malpighian tubules of drosophila auraria larvae. periodate-reactive (neutral) polysaccharides were present in the foregut cuticle, peritrophic membrane, cells of the proventriculus which produce the peritrophic membrane, as well as the glycocalyx of the midgut and malpighian tubule cells. sulphated and carboxylated complex carbohydrates were detected in the cuticle of both ... | 1992 | 1283121 |
| developmental changes in fat body and midgut chromosomes of drosophila auraria. | changes in puffing activity of fat body (fb) and midgut (mg) chromosomes of drosophila auraria during late larval and white prepupal development as well as after in vitro culture with or without ecdysterone were studied and compared with those of the salivary gland (sg). the balbiani rings characteristic of the sg chromosomes of d. auraria, are not formed in fb and mg. most of the inverted tandem chromosomal duplications that have been found to be common to all three tissues showed differentiati ... | 1991 | 1914663 |
| evolutionary implications of duplications and balbiani rings in drosophila. a study of drosophila serrata. | drosophila serrata, a species of subgroup montium, exhibits in its salivary-gland chromosomes a high number of inverted tandem duplications and a well-formed balbiani ring. a photographic map, the duplications, and the balbiani ring of this species are presented. also presented are the most prominent puffs during normal larval development and after ecdysone treatment, as well as intercalary heterochromatin regions of the above chromosomes. these data are discussed and compared with those of dros ... | 1990 | 2227403 |
| temperature dependence and evolutionary adjustment of critical night length in insect photoperiodism. | the photoperiodic responses of drosophila auraria are shown to involve its circadian system functioning as the "clock" that measures the duration of darkness at night. attempts at further clarification of this finding were based on the widely held assumption that adaptive adjustment of critical night length is caused by change in the circadian system's entrainment behavior. three different experimental programs yielded data that are incompatible with this starting premise. collectively, the obse ... | 1987 | 16593882 |
| possible correlations of polypeptides and balbiani rings in the salivary glands of drosophila auraria peng. | salivary glands of various stocks of drosophila auraria and some of its close relatives were examined with a variety of electrophoretic techniques both from larval and prepupal stages, and after ecdysterone treatments, in an effort to detect possible translation products of the two balbiani rings (br1 and br2) found in the salivary gland chromosomes of these species. two polypeptides (p2 and p1), with molecular weights of 12,000 and 53,000, respectively, have been detected, the appearance of whi ... | 1987 | 3506528 |
| dilated cisternae of nuclear envelope and rough endoplasmic reticulum in the proventriculus of drosophila auraria larvae. | in the cells of the middle layer of the proventriculus of drosophila auraria larvae, the nuclear envelope and the rough endoplasmic reticulum are always found in the form of dilated cisternae. the length of these cisternae can reach 10 mu. there are indications that materials from the outer membrane of the nuclear envelope are directly transported to the golgi complex of the examined cells. | 1985 | 4028199 |
| ultrastructural analysis of peritrophic membrane formation of drosophila auraria larvae. | the cells of the external layer of the proventriculus of drosophila auraria larvae, which produce the peritrophic membrane are characterized by bears microvilli, polytenic nuclei, large quantities of ribosomes, very well developed rough endoplasmic reticulum and many golgi complexes. the examined cells present one of the rare exceptions of insect intestinal cells, that form a prominent secretory product. the material of the peritrophic membrane, that gives a positive neutral polysaccharide react ... | 1985 | 3999190 |
| effect of he-ne laser on the midgut cells of drosophila auraria larvae and its correlation with acupuncture. | the he-ne laser is generally considered as a "cold" or "soft" laser. however, the present study indicates that doses from 0.3 to 0.5 j/cm2 of he-ne laser caused intensive damage that was mainly observed on the plasma membrane and the mitochondria of the midgut cells of drosophila auraria larvae. in contrast, doses less than 0.3 j/cm2 of the he-ne laser didn't cause any visible effect, as measured by the electron microscope. the possible use of the he-ne laser and the subsequent effect in relatio ... | 1985 | 2861722 |
| acid phosphatases in drosophila auraria. | a study was carried out dealing with the acid phosphatases, in larvae, pupae and adults of drosophila auraria. the most interesting finding is that the acid phosphatases are observed in 3 different phenotypes appearing as groups of 3 bands of different mobilities in the homozygotes and giving a 5 band phenotype in the heterozygote. a hypothesis is discussed in an attempt to explain the phenotypes observed and their variation during the development of the species. | 1976 | 976440 |
| behavioral differentiation of three races of drosophila auraria. | | 2006 | 5111029 |