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[morphofunctional features of the development of the hypothalamo-hypophyseal neurosecretory system and adenohypophysis in the round goby gobius melanostomas pallas during early ontogenesis].the formation of the hypothalamo-hypophysial neurosecretory system (hhns) and adenohypophysis in the round goby takes place during the embryogenesis. the hhns functional differentiation begins earlier than the anatomical one. the homori-positive neurosecretory material was found in the neurohypophysis of one day old larvae. the first, within 7-10 days after the hatching, to be differentiated are the acidophilic cells of mesoadenohypophysis, then, on the 10-15th day, the acidophilic cells of proa ...1977904848
[chromosomes of the black sea gobiidae--gobius melanostomus (pallas) and gobius batrachocephalus (pallas) ].the chromosome set of g. melanostomus (pallas) consists of 46 acrocentric chromosomes (2n-46). there are 30 chromosomes in the caryotype of g. batrachocephalus (pallas): 16 metacentric and 14 acrocentric. the equal number of arms in the chromosome sets of the studied fish (fn-46) evidences for phylogenetic relationships of these species.19751221581
[distribution of gomori-positive neurosecretion in the hypothalamo-hypophyseal system of gobius melanostomus pallas]. 19684195485
[change in the antigenic composition of the hypophysis of gobius melanostomus in relation to its sexual cycle]. 19734202091
[some data of a morphological comparison of the hypophyses of gobius melanostomus pall. and gobius batrachocephalus pall. characterized by intermittent and single spawning]. 19714327406
[morphophysiologic characteristics of the hypophysis in the round fish (gobius melanostomus pallas) in connection with the reproductive cycle]. 19695364910
[adaptive changes of the retina in ontogeny of gobius melanostomus (pall.)]. 19715565960
[sex differences in the pituitary gonadotropic function in gobius melanostomus pallas]. 19817261874
[change in the activity of antioxidant enzymes in the process of early ontogenesis of various species of black sea fishes].the dynamics of activities of lipoxygenase, superoxide dismutase, catalase, proxidase and glutathione reductase during the development of eggs and hatched larvae of five species of black sea fishes was studied. the increase of enzyme activities at the end of embryogenesis and in the larvae was established. high correlations between the activities of superoxide dismutase, catalase and glutathione peroxidase (r > 0.95) and the average coefficients for neogobius melanostomus (0.4957) for proterorhi ...19958588261
[dioxins and furans in edible species of fish from the gulf of gdańsk].the levels of pcdds and pcdfs in samples of lamprey, perch, herring, cod, flounder, round goby, eelpout and pikeperch caught in the gulf of gdańsk were determined by hrgc/hrms. the results are expressed as tcdd equivalents, calculated according to international (i-teqs), nordic (n-teqs) models. the levels (pg teqs/g wet weight) were from 0.263 in lamprey to 6.047 in herring (i-teqs). except of herring nearly all fish species sampled contained pcdds and pcdfs in concentration below 1 pg teqs/g we ...19969064741
parasites of the recently established round goby (neogobium melanostomus) and tubenose goby (proterorhinus marmoratus) (gottidae) [corrected] from the st. clair river and lake st. clair, michigan, usa. 19979229568
putative steroidal pheromones in the round goby, neogobius melanostomus: olfactory and behavioral responses.to identify potential hormonal pheromones of the round goby (neogobius melanostomus), a species recently introduced to the great lakes, we used electro-olfactogram (eog) recording to examine olfactory responsiveness to more than 100 steroids and prostaglandins. neogobius detected free and conjugated 18-, 19- and 21-carbon steroids, but did not detect prostaglandins. eog cross-adaptation, used to determine if neogobius can discriminate the detected compounds at the sensory level, suggested that t ...200111441438
chlorinated cyclodiene pesticide residues in blue mussel, crab, and fish in the gulf of gdańsk, baltic sea.chlordane components (chls) and their metabolites (heptachlor, cis-heptachlor epoxide, u82, mc4, trans-chlordane, mc5, cis-chlordane, mc7, oxychlordane, mc6- and trans- and cis-nonachlor) and aldrin, dieldrin, endrin, isodrin, endosulfan 1, endosulfan 2, and mirex were quantified in the soft tissues of blue mussel, a whole crab, and whole fishes collected from the spatially different sites in the gulf of gdańsk. six to twelve chlordane compounds and metabolites and dieldrin were detected in all ...200111718327
methyl-testosterone induces male-typical ventilatory behavior in response to putative steroidal pheromones in female round gobies (neogobius melanostomus).male round gobies (neogobius melanostomus) transiently increase their rate of ventilation when exposed to nanomolar concentrations of three putative steroidal pheromones (etiocholanolone, etio; estrone, e1; estradiol-glucuronide, e2-3g), whereas females exhibit this response only to etio. in this study we implanted females with silastic capsules that were empty (blank) or filled with methyl-testosterone (mt) to determine whether androgen induces a male-typical pattern of ventilatory response. as ...200212367564
morphology and histochemistry of the peripheral olfactory organ in the round goby, neogobius melanostomus (teleostei: gobiidae).this first comprehensive study of the peripheral olfactory organ from a representative of the large and economically important order of teleost fishes, the perciformes, shows a compact structure with olfactory sensory neurons distributed widely throughout the olfactory chamber. the spatial organization of the nasal cavity in the bottom-dwelling round goby (gobiidae, neogobius melanostomus) was examined using impression material injection, immunocytochemistry, and transmission electron microscopy ...200312740897
polychlorinated naphthalenes and polychlorinated biphenyls in benthic organisms of a great lakes food chain.invasion of zebra mussels, dreissena polymorpha, and round gobies, neogobius melanostomus, into the great lakes has altered the food web structure and thereby the pathways of toxic contaminants such as polychlorinated biphenyls (pcbs) and polychlorinated naphthalenes (pcns). in this study, concentrations of pcns and pcbs were measured in organisms of a great lakes benthic food chain encompassing zebra mussels. pcns were found in all of the benthic organisms, including phytoplankton, algae, amphi ...200415346781
in vitro biosynthesis of novel 5beta-reduced steroids by the testis of the round goby, neogobius melanostomus.previous studies indicate that, in the round goby neogobius melanostomus, the reproductively mature male releases a pheromone that attracts ripe females. furthermore, studies suggest that the pheromone may be a steroid (more specifically a 5beta-reduced androgen) produced by specialized glandular tissue in the testes. in the present study, it is shown that the testis of the male round goby contains such specialized glandular tissue. in vitro, the testes convert [3h]androstenedione into 3alpha-hy ...200515596066
perfluorinated compounds in aquatic organisms at various trophic levels in a great lakes food chain.trophic transfer of perfluorooctanesulfonate (pfos) and other related perfluorinated compounds was examined in a great lakes benthic foodweb including water-algae-zebra mussel-round goby-smallmouth bass. in addition, perfluorinated compounds were measured in livers and eggs of chinook salmon and lake whitefish, in muscle tissue of carp, and in eggs of brown trout collected from michigan. similarly, green frog livers, snapping turtle plasma, mink livers, and bald eagle tissues were analyzed to de ...200515883668
modeling the effects of chronic toxicity on fish populations: the influence of life-history strategies.five life-history scenarios were composed to simulate fish population adaptations for survival under various environmental conditions. the scenarios encompassed differences in stage-specific survival, fecundity and hatch success, number of spawning events, and life-span effect response to chronic toxic impacts. the strategies represent a good survivor/generalist (gs), a species exhibiting high young-of-the-year survival, a species exhibiting high adult survival, a species with no parental care o ...200516117134
trophic transfer and biotransformation of polychlorinated biphenyls in zebra mussel, round goby, and smallmouth bass in lake erie, usa.trophic transfer of polychlorinated biphenyl (pcb) congeners in zebra mussels (dreissena polymorpha), round gobies (neogobius melanstomus), and smallmouth bass (micropterus dolomieu) were assessed in four sites along the south shore of the west and central basin of lake erie (all sites were in oh, usa). total pcb levels in smallmouth bass (1,091-1,520 ng/g wet weight) and round gobies (118-256 ng/g wet weight) were similar among sites despite a west-to-east decrease in total pcb concentrations i ...200616629146
olfactory sensory input increases gill ventilation in male round gobies (neogobius melanostomus) during exposure to steroids.in teleostean fish, ventilation increases have been observed in response to low dissolved oxygen levels, visual stimuli, and gustatory cues. however, olfactory sensory input may also stimulate gill ventilation rate. we investigated whether olfactory sensory input mediates gill ventilation responses, as suggested by the observation that steroidal compounds detected by the olfactory system elicited increases in opercular activity in the perciform teleost, the round goby (neogobius melanostomus). c ...200616631399
persistence of clostridium botulinum neurotoxin type e in tissues from selected freshwater fish species: implications to public health.rainbow trout (oncorhynchus mykiss), round gobies (neogobius melanostomas), yellow walleye (stizostedion vitreum), and yellow perch (perca flavescens) were given clostridium botulinum neurotoxin type e (bont/e) at four doses (0, 800, 1500, and 4000 mouse lethal doses). bont/e was sought in the fish tissues at death or at the conclusion of the experiment (10 days after treatment). fish were divided into a "fillet" (axial musculature) and a "nonfillet" sample before testing for bont/e toxicity wit ...200616715821
the lesser evils of battling round goby infiltration. 200616717263
colonization of the round goby, neogobius melanostomus (gobiidae) by parasites in the new environment of the gulf of gdańsk (southern baltic).the round goby (neogobius melanostomus) is a non-indigenous species in the baltic sea, introduced to its waters (the gulf of gdańsk) from the black, azov, and caspian seas. for this reason, an attempt was made to determine the species' parasitic fauna in its new environment. within 1994-2000, a total of 201 round goby specimens caught in the gulf of gdańsk were examined. the parasites found represented protozoans (trichodina domerguei domerguei), digeneans (diplostomum spp. metacercariae), cesto ...200216888941
metazoa parasites of the invasive round goby apollonia melanostoma (neogobius melanostomus) (pallas) (gobiidae: osteichthyes) in the gulf of gdańsk, baltic sea, poland: a comparison with the black sea.the known metazoa parasite fauna of the invasive round goby apollonia melanostoma (formerly neogobius melanostomus) consists of 12 species. the core of the parasite fauna comprises two species: cryptocotyle concavum and diplostomum spathaceum; secondary species are absent; satellite species include cercariae gen. sp. and ergasilus sieboldi; rare species are acanthocephalus lucii, anguillicola crassus, bothriocephalus sp., dichelyne minutus, hysterothylacium aduncum, pomphorhynchus laevis, piscic ...200717048001
toxicity of clostridium botulinum type e neurotoxin to great lakes fish: implications for avian botulism.since 1999, large-scale mortalities of fish-eating birds have been observed on the great lakes, and more specifically on lake erie. type e botulism has been established as the primary cause of death. the mechanism of type e botulism exposure in fish-eating birds is unclear. given that these birds are thought to eat live fish exclusively, it seems likely that their prey play a key role in the process, but the role of fish as potential transport vectors of botulinum neurotoxin type e (bont/e) to b ...200617092878
repeated low-level exposure of the round goby (neogobius melanostomas) to clostridium botulinum type e neurotoxin.in a 4-mo study (june 2004-september 2004), round gobies (neogobius melanostomas) were dosed orally every 72 hr for up to 21 days with clostridium botulinum neurotoxin type e (bont/e) at one of four doses: 0, 50, 250, and 500 mouse lethal doses (mld). fish were observed for changes in pigmentation and behavior for the duration of the experiment. mortality was observed with all treatments, with the exception of the 0 mld control. clinical signs observed were consistent with prior research and app ...200617092879
differential behavioral responses by reproductive and non-reproductive male round gobies (neogobius melanostomus) to the putative pheromone estrone.previous studies have shown that the frequency of gill ventilation during exposure to estrone and gonadal extracts in the round goby (neogobius melanostomus) is linked to olfactory sensory input. control over gill ventilation may be a regulatory mechanism used for odorant sampling during reproductive periods. in this study, we examined changes in gill ventilation in osmic and anosmic (nasal occluded), reproductive and non-reproductive male round gobies to a putative steroidal pheromone estrone ( ...200717293144
use of chemical communication in the management of freshwater aquatic species that are vectors of human diseases or are invasive.chemical communication occurs when both originator (signaller) and one or more receiver(s) possess specializations for chemical exchange of information. chemical information can be used by a wide variety of species to locate food and mates, avoid predators and engage in social interactions. in this review, we focus on chemical signalling between mates or cues from nest sites or hosts by selected aquatic pest species and indicate how chemical information can be used to manage pests. the pests are ...200717367788
the seminal vesicle synthesizes steroids in the round goby neogobius melanostomus.in this study, we examine the possible contribution of the seminal vesicles of the male round goby to the production of putative steroidal pheromones. a previous study showed that the testes of the round goby are rich in steroid-producing leydig-like cells; and when incubated in vitro, convert tritiated androstenedione to at least six other steroids, including one not previously identified in fish--namely 3alpha-hydroxy-5beta-androstane-11,17-dione (11-oxo-etiocholanolone, 11-oxo-etio). the semi ...200717512768
[convergence of early ontogeneses of the teleostean fish neogobius melanostomus (perciformes, gobiidae) and higher vertebrates].in the teleostean fish round goby with nonlarval type of development (anamnia) and even-toed mammals pig and sheep (amniota), the processes of digestion during early ontogenesis are similar, which is correlated with the parental care and embryonization of development.200717668466
mortality event in freshwater drum aplodinotus grunniens from lake ontario, canada, associated with viral haemorrhagic septicemia virus, type iv.a mortality event primarily affecting freshwater drum aplodinotus grunniens was noted during april and may 2005 in the bay of quinte, lake ontario, canada. a conservative estimate of the number of dead drum was approximately 100 metric tonnes. large numbers of dead round goby neogobius melanostomus were also seen, as well as a few muskellunge esox masquinongy. in the drum, there was a consistent histological pattern of variably severe panvasculitis, a necrotising myocarditis, meningoencephalitis ...200717760383
detection of viral hemorrhagic septicemia in round gobies in new york state (usa) waters of lake ontario and the st. lawrence river.in may 2006 a large mortality of several thousand round gobies neogobius melanostomus (pallas, 1814) occurred in new york waters of the st. lawrence river and lake ontario. necropsies of sampled fish from these areas showed pallor of the liver and gills, and hemorrhagic areas in many organs. histopathologic examination of affected tissues revealed areas of necrosis and hemorrhage. inoculations of fathead minnow pimephales promelas (rafinesque, 1820) cell cultures with dilutions of tissue samples ...200717803104
molecular identification of two strains of third-stage larvae of contracaecum rudolphii sensu lato (nematoda: anisakidae) from fish in poland.contracaecum sp. larvae (l3) from fish were identified using nucleotide sequences of the internal transcribed spacers its-1 and its-2 of the ribosomal dna. the nematode larvae originated from fish in a freshwater situation (crucian carp carassius carassius, from selment wielki lake in mazury, northeastern poland) and a brackish-water region (caspian round goby neogobius melanostomus from the baltic sea, gdafisk bay at the polish coast). two strains (contracaecum rudolphii a and b) of contracaecu ...200717918390
ancient divisions, recent expansions: phylogeography and population genetics of the round goby apollonia melanostoma.during the past two decades, the round goby apollonia melanostoma (=neogobius melanostomus) has expanded its range via shipping transport and canals, extending north and west from the ponto-caspian region of eurasia and to the north american great lakes. exotic populations of the round goby have been very successful in the baltic sea and the great lakes regions, exerting significant ecological changes. our study evaluates the population genetic and biogeographical structure of the round goby acr ...200818466236
effect of diel period and season on seining effort required to detect changes in lake erie beach fish assemblages.using beach seining data from lake erie beaches, we examined the effect of diel period and season on two common study objectives considered sensitive to sampling effort: (1) has fish assemblage composition changed between two sampling time periods?, and (2) how many samples are necessary to provide a representative sample of the fish assemblage? across a range of effect sizes and power levels, sample sizes were estimated for species richness, total fish abundance, round goby (neogobius melanosto ...200918491208
chemical amplification in an invaded food web: seasonality and ontogeny in a high-biomass, low-diversity ecosystem.the global spread of invasive species is changing the structure of aquatic food webs worldwide. the north american great lakes have proved particularly vulnerable to this threat. in nearshore areas, invasive benthic species such as dreissenid mussels and round gobies (neogobius melanostomus) have gained dominance in recent years. such species are driving the flow of energy and material from the water column to the benthic zone, with dramatic effect on nutrient and contaminant cycling. here, we d ...200818544008
temporal and spatial patterns of contaminants in lake erie watersnakes (nerodia sipedon insularum) before and after the round goby (apollonia melanostomus) invasion.temporal and spatial trends in contaminant concentrations were assessed in lake erie watersnakes, a threatened (usa)/endangered (canada) species restricted to western lake erie. temporal changes in plasma contaminant levels were determined in 1990 and 2003, and spatial patterns in 2003 at 12 sites, throughout the species' range. during this period, the watersnakes' diet changed from fish (75%) and amphibians (25%) that avoid zebra mussels, to round gobies (95%) that feed extensively on zebra mus ...200818771794
invasion genetics of the eurasian round goby in north america: tracing sources and spread patterns.the eurasian round goby neogobius melanostomus (apollonia melanostoma) invaded the north american great lakes in 1990 through ballast water, spread rapidly, and now is widely distributed and moving through adjacent tributaries. we analyse its genetic diversity and divergence patterns among 25 north american (n = 744) and 22 eurasian (n = 414) locations using mitochondrial dna cytochrome b gene sequences and seven nuclear microsatellite loci in order to: (i) identify the invasion's founding sourc ...200919140965
effects of vitamin b1 (thiamine) deficiency in lake trout (salvelinus namaycush) alevins at hatching stage.the objectives of this study were to examine the relationship between thiamine concentrations in unfertilized eggs and yolksac individuals of lake trout (salvelinus namaycush), along with any associated histopathological changes in the tissues of alevins at the hatching stage. we address these questions in a lake trout population from different spawning grounds of lake michigan (north and south), known for compromised survival due to early mortality syndrome (ems). however, a dichotomous forage ...200919576291
[haemoglobin system of black sea round goby under experimental hypoxia conditions].haemoglobin of the black sea round goby (neogobius melanostomus p.) resisting to external hypoxia was studied. haemoglobin of this species was fractionated into 4 components (2 minor and 2 major ones) by the disk-electrophoresis in polyacrylamid gel. the major fractions were eluted and their respiration parameters were investigated. one of them showed high affinity for o2 (p50 5.81 +/- 0.74 mm hg at ph 8.3) and heightened sensibility to ph (bohr effect amounted to -0.81 +/- 0.09 at ph of 8.3 --> ...200419621744
parasites of apollonia melanostoma (pallas 1814) and neogobius kessleri (guenther 1861) (osteichthyes, gobiidae) from the danube river in austria.two invasive fish species, the round goby apollonia melanostoma syn. neogobius melanostomus (pallas 1814) and the bighead goby neogobius kessleri (günther, 1861), have established a firm population in austrian waters during the past 15 years. as there have been no records of the parasite fauna from these populations, a total of 79 specimens of a. melanostoma and 12 specimens of n. kessleri were examined for parasites between may and october 2007 from three different sampling sites from the danub ...201019627624
bucephalus polymorphus baer, 1827 - a new fish parasite in austria?during parasitological studies of the invasive round goby fish species apollonia melanostoma (pallas, 1814) from the danube river in 2007 the digenean bucephalus polymorphus baer, 1827 was detected for the first time in austria. encysted metacercariae of the parasite were found on the fins, skin and gills of the fish. the occurrence of the parasite is strictly dependant on the zebra mussel dreissena polymorpha (pallas, 1771), which acts as the obligatory first intermediate host in its life cycle ...200919915818
the use of surrogate species in risk assessment: using life history data to safeguard against false negatives.the use of surrogate species is an important tool in predicting the effects of management decisions or the establishment of protective measures for endangered/threatened species. while relying on a handful of model species to predict the fate of scores of distantly related target species has been criticized, a quantitative measure linking life history traits and population predictions has been sorely missing. we derive here a closed-form expression aimed at determining conditions under which sub ...201020136742
influence of thiamine deficiency on lake trout larval growth, foraging, and predator avoidance.diet-related thiamine deficiency increases the acute mortality, known as early mortality syndrome, of salmonines from some of the great lakes. the consequences of thiamine deficiency as measured at the egg stage for other important early life stage processes like growth, foraging efficiency, and predator avoidance that may also result in mortality, are unknown. accordingly, we investigated the impacts of low thiamine on the specific growth rate (sgr) of first-feeding fry, the ability of first-fe ...200920218504
signatures of contamination in invasive round gobies (neogobius melanostomus): a double strike for ecosystem health?the invasive round goby has a recognised role in transferring contaminants through foodwebs, but little work has been done on contaminant impacts on round gobies themselves. here we present the first case study of contaminant biomarkers and subpopulation structure variation in round gobies, in relation to habitat contamination, within a canadian area of concern, hamilton harbour. copper and cadmium were elevated in livers of fish from contaminated habitats. although catch abundances were similar ...201020615549
characterization of vitellogenin gene expression in round goby (neogobius melanostomus) using a quantitative polymerase chain reaction assay.a growing concern over endocrine disruption in aquatic species has prompted the development of molecular assays to monitor environmental impacts. this study describes the development of quantitative polymerase chain reaction (qpcr) assays to characterize the expression of two vitellogenin (vtg) genes in the invasive round goby (neogobius melanostomus). fragments from the 18srrna (housekeeping gene), vtg ii, and vtg iii genes were cloned and sequenced. the qpcr assays were developed to detect hep ...201020891019
release of free and conjugated forms of the putative pheromonal steroid 11-oxo-etiocholanolone by reproductively mature male round goby (neogobius melanostomus pallas, 1814).previous studies of the round goby (neogobius melanostomus pallas, 1814), an invasive fish species in the laurentian great lakes of north america, have shown that this species has the ability to both synthesize and smell steroids that have a 5 beta-reduced and 3 alpha-hydroxyl (5 beta,3 alpha) configuration. an enzyme-linked immunoassay (eia) for 3 alpha-hydroxy-5 beta-androstane-11,17-dione (11-o-etio) has been used to show a substantial rise in the rate of release of immunoreactive compounds i ...201020944082
the effect of stimulus type and background noise on hearing abilities of the round goby neogobius melanostomus.the auditory abilities of the round goby neogobius melanostomus were quantified using auditory evoked potential recordings, using tone bursts and conspecific call stimuli. fish were tested over a range of sizes to assess effects of growth on hearing ability. tests were also run with and without background noise to assess the potential effects of masking in a natural setting. neogobius melanostomus detected tone bursts from 100 to 600 hz with no clear best frequency in the pressure domain but wer ...201021078014
detection of the spatiotemporal trends of mercury in lake erie fish communities: a bayesian approach.the temporal trends of total mercury (thg) in four fish species in lake erie were evaluated based on 35 years of fish contaminant data. our bayesian statistical approach consists of three steps aiming to address different questions. first, we used the exponential and mixed-order decay models to assess the declining rates in four intensively sampled fish species, i.e., walleye (stizostedion vitreum), yellow perch (perca flavescens), smallmouth bass (micropterus dolomieui), and white bass (morone ...201121329342
the effect of elevated steroids released by reproductive male round gobies, neogobius melanostomus, on olfactory responses in females.the round goby, neogobius melanostomus, is a highly successful invasive species in the laurentian great lakes. previous behavioral studies implied that females are attracted by pheromones to the nests of reproductive males, and that males release putative steroidal pheromones--unconjugated as well as conjugated forms of 3a-hydroxy-5ß-androstane-11,17-dione (11-o-etio)-following stimulation of the hypothalamic--gonadal axis with salmon gonadotropin releasing hormone analog (sgnrha). in this study ...201121365215
the colonization of the invasive round goby neogobius melanostomus by parasites in new localities in the southwestern baltic sea.the round goby neogobius melanostomus is a bottom-dwelling fish native for the ponto-caspian basin, which started to colonize the baltic sea since 1991. the parasites of this fish species in the southwestern baltic region are studied for the first time. the round goby in the sw baltic sea was infected with 12 parasite species: 1 species of microsporidia, 1 species of monogenea, 1 of cestoda, 3 species of trematoda, 3 species of nematoda, 2 of acanthocephala, and 1 species of bivalvia. microspori ...201121409440
dispersal strategies, secondary range expansion and invasion genetics of the nonindigenous round goby, neogobius melanostomus, in great lakes tributaries.dispersal strategies are important mechanisms underlying the spatial distribution and colonizing ability of all mobile species. in the current study, we use highly polymorphic microsatellite markers to evaluate local dispersal and colonization dynamics of the round goby (neogobius melanostomus), an aquatic invader expanding its range from lake to river environments in its introduced north american range. genetic structure, genotype assignment and genetic diversity were compared among 1262 round ...201121492265
a novel approach to avt and it studies in fish brain and pituitary: in vitro perfusion technique.the study was designed to develop a new procedure for perfusion of brain and pituitary explants collected from three-spined stickleback (gasterosteus aculeatus) and round goby (neogobius melanostomus). the procedure was elaborated for studies of arginine vasotocin (avt) and isotocin (it) release from explants of both species. avt and it, analogs of mammalian vasopressin and oxytocin, are neurohormones produced in hypothalamus and released in neurohypophysis of teleostei. both nonapeptides are us ...201121569795
round goby neogobius melanostomus attraction to conspecific and heterospecific egg odours.in this study, cannibalistic and predatory responses of juvenile neogobius melanostomus (either fed or deprived of food and caught during the reproductive summer or non-reproductive winter season) to water washings of fertilized eggs from conspecifics (n. melanostomus), heterospecifics (rainbow trout oncorhynchus mykiss) and control lake water were tested. fed juveniles (summer season) spent significantly more time on the side of the flume containing stimuli from conspecific eggs over control wa ...201121651542
age and growth of the round goby neogobius melanostomus in the gulf of gdańsk several years after invasion. is the baltic sea a new promised land?the ages of 8 to 23·5 cm total length (l(t)) round goby neogobius melanostomus collected monthly during 2006 and 2007 in the gulf of gdańsk (baltic sea) ranged from 2 to 6 years, with age class 4+ years dominant. males were larger at age than females. the fastest growth occurred in the first 2 years of life in both sexes. females were heavier at a given l(t) than males, but only for fish > c. 15 cm. a strong relationship between n. melanostomus otolith size and fish size was found, with no diffe ...201121651546
responses of round goby (neogobius melanostomus) olfactory epithelium to steroids released by reproductive males.the wild perciform teleost neogobius melanostomus (the round goby) originated from the ponto-caspian region and is now a highly successful invasive species in the laurentian great lakes. males may attract females into their nests for spawning by releasing reproductive pheromones, and it has been previously shown that reproductive males synthesize and release the 5+¦-reduced and 3+¦-hydroxyl steroids 3+¦-hydroxy-5+¦-androstane-11,17-dione (11-oxo-etiocholanolone; 11-o-etio) and 3+¦-hydroxy-5+¦-an ...201121735225
twenty years of invasion: a review of round goby neogobius melanostomus biology, spread and ecological implications.the round goby neogobius melanostomus is one of the most wide-ranging invasive fish on earth, with substantial introduced populations within the laurentian great lakes watershed, the baltic sea and several major european rivers. rapid expansion and deleterious ecosystem effects have motivated extensive research on this species; here this research is synthesized. maps of the global distribution are provided and the invasion history of n. melanostomus, which spread more rapidly at first in north a ...201222268429
temporal patterns in growth and survival of the round goby neogobius melanostomus.monthly, overwinter and annual instantaneous growth rates for round goby neogobius melanostomus were calculated with maximal growth occurring in july and august and almost no growth observed between ice appearance (october) and melt (march). annual absolute growth rates averaged 27·3 ± 1·9 mm for males and 19·8 ± 2·4 mm for females. the most parsimonious cormack-jolly-seber model indicated that both the survival and recapture probabilities were dependent on sampling date, but not sex. survival e ...201223331141
invasive ponto-caspian amphipods and fish increase the distribution range of the acanthocephalan pomphorhynchus tereticollis in the river rhine.non-indigenous species that become invasive are one of the main drivers of biodiversity loss worldwide. in various freshwater systems in europe, populations of native amphipods and fish are progressively displaced by highly adaptive non-indigenous species that can perform explosive range extensions. a total of 40 ponto-caspian round gobies neogobius melanostomus from the rhine river near düsseldorf, north rhine-westphalia, germany, were examined for metazoan parasites and feeding ecology. three ...201223300895
nematode eel parasite found inside acanthocephalan cysts--a "trojan horse" strategy?the invasive eel parasite anguillicoloides crassus (syn. anguillicola crassus) is considered one of the major causes for the decline of the european eel (anguilla anguilla) panmictic population. it impairs the swim bladder function and reduces swimming performance of its host. the life cycle of this parasite involves different intermediate and paratenic hosts. despite an efficient immune system of the paratenic fish hosts acting against infections with a. crassus, levels of parasitized eels rema ...201425403767
non-native gobies facilitate the transmission of bucephalus polymorphus (trematoda).introduced species can modify local host-parasite dynamics by amplifying parasite infection which can 'spill-back' to the native fauna, whether they are competent hosts for local parasites, or by acting as parasite sinks with 'dilution' of infection decreasing the parasite burden of native hosts. recently infection by the trematode bucephalus polymorphus has increased in several european rivers, being attributed to the introduction of intermediate host species from the ponto-caspian region. usin ...201526187653
survey of viral haemorrhagic septicaemia virus in wild fishes in the southeastern black sea.species diversity in the black sea ecosystem has been declining rapidly over the last 2 decades. to assess the occurrence and distribution of viral haemorrhagic septicaemia virus (vhsv) in various wild fish species, a wild marine fish survey was carried out in 2009, 2010, and 2011. the pooled or individual samples of kidney, liver, and spleen of 5025 specimens, belonging to 17 fish species, were examined virologically using cell culture. the cells showing cytopathic effects (cpe) were subjected ...201424991737
study of loma acerinae (microsporidia) detected from three ponto-caspian gobies (gobiidae) in ukraine.a previously recognized glugea-belonging microsporidia (g. melanostomi, g. markievichi, g. naidenovi) were found in the intestinal mucosa of neogobius melanostomus, n. fluviatilis, and proterorhinchus semilunaris (teleostei, gobiidae) from lower dnieper river and black sea coastal inlets, ukraine. light microscope observations demonstrated small spherical xenomes about 0.35 mm in diameter filled by developmental stages of parasites. mature spores were ellipsoidal, slightly narrowed at anterior p ...201728331963
first detection of circovirus-like sequences in amphibians and novel putative circoviruses in fishes.the negative samples of a collection, established originally for seeking new adeno- and herpesviruses in lower vertebrates, were screened for the pres-ence of circoviruses by a consensus nested pcr targeting the gene coding for the replication-associated protein. six fish samples representing five species, namely asp (aspius aspius), roach (rutilus rutilus), common bream (abramis brama), round goby (neogobius melanostomus) and monkey goby (neogobius fluviatilis), as well as three frog samples we ...201424334078
getting what is served? feeding ecology influencing parasite-host interactions in invasive round goby neogobius melanostomus.freshwater ecosystems are increasingly impacted by alien invasive species which have the potential to alter various ecological interactions like predator-prey and host-parasite relationships. here, we simultaneously examined predator-prey interactions and parasitization patterns of the highly invasive round goby (neogobius melanostomus) in the rivers rhine and main in germany. a total of 350 n. melanostomus were sampled between june and october 2011. gut content analysis revealed a broad prey sp ...201425338158
in vivo and in vitro phenotypic differences between great lakes vhsv genotype ivb isolates with sequence types vcg001 and vcg002.viral hemorrhagic septicemia virus (vhsv) is an aquatic rhabdovirus first recognized in farmed rainbow trout in denmark. in the past decade, a new genotype of this virus, ivb was discovered in the laurentian great lakes basin and has caused several massive die-offs in some of the 28 species of susceptible north american freshwater fishes. since its colonization of the great lakes, several closely related sequence types within genotype ivb have been reported, the two most common of which are vcg0 ...201425722533
experimental transmission of vhsv genotype ivb by predation.preliminary surveillance of wild baitfish during the 2006 viral hemorrhagic septicemia virus genotype ivb (vhsv ivb) outbreaks indicated emerald shiners notropis atherinoides and bluntnose minnow pimephales notatus were infected with high levels of vhsv without showing clinical signs of disease. the movement and use of baitfish was recognized as the most probable vector for the introduction of vhsv to inland waters, such as conesus lake and skaneateles lake in new york, budd lake in michigan, an ...201323998650
neuropeptides isotocin and arginine vasotocin in urophysis of three fish species.in this study, for the first time, both neuropeptides isotocin (it) and arginine vasotocin (avt) have been identified and measured in urophysis, the neurohaemal organ of the caudal neurosecretory system of teleost fish. so far, avt, but not it, was quantified by radioimmunoassay (ria) in urophysis of several fish species. we have used high-performance liquid chromatographic assay with fluorescence detection (hplc-fl) preceded by solid-phase extraction (spe) and liquid chromatography-electrospray ...201323142930
metals in edible fish from vistula river and dead vistula river channel, baltic sea.metals including al, ba, ca, cd, co, cr, cu, fe, hg, k, mg, mn, na, ni, pb, sr and zn were determined in muscle tissue of 12 fish species by inductively coupled plasma-atomic emission spectroscopy (icp-aes) and cold vapour-atomic absorption spectroscopy (cv-aas). fish were collected from vistula river at lower course and dead vistula river channel in south of baltic sea in poland. the fish species examined include round goby (neogobius melanostomus), crucian carp (carassius carassius), bull-rout ...201222428891
toxic metals in tissues of fishes from the black sea and associated human health risk exposure.the anthropogenic activities in the black sea area are responsible for toxic metal contamination of sea food products. in this study, several toxic metals: cadmium, lead, nickel, chromium, and copper were quantified in different tissues (digestive tract, muscle, skeleton, skin) of nine fish species (neogobius melanostomus, belone belone, solea solea, trachurus mediterraneus ponticus, sardina pilchardus, engraulis encrasicolus, pomatomus saltatrix, sprattus sprattus, scorpaena porcus) by using at ...201728127691
the use of environmental dna in invasive species surveillance of the great lakes commercial bait trade.over 180 non-native species have been introduced in the laurentian great lakes region, many posing threats to native species and ecosystem functioning. one potential pathway for introductions is the commercial bait trade; unknowing or unconcerned anglers commonly release unused bait into aquatic systems. previous surveillance efforts of this pathway relied on visual inspection of bait stocks in retail shops, which can be time and cost prohibitive and requires a trained individual that can rapidl ...201525169113
dietary habits of invasive ponto-caspian gobies in the croatian part of the danube river basin and their potential impact on benthic fish communities.invasive ponto-caspian (p-c(1)) gobies have recently caused dramatic changes in fish assemblage structures throughout the danube basin. while their presence in the croatian part of the basin has been noted and distribution studied, their dietary habits and impacts on native fish communities have, until now, been unknown. in 2011, 17 locations in the sava river basin were sampled for fish and 15 for benthic invertebrates. fish population monitoring data, available for nine seasons (2003-2006 and ...201626054972
fish pollution with anthropogenic 137cs in the southern baltic sea.this paper presents the results of a study on changes in (137)cs activity concentrations in three fish species from the southern baltic sea: cod (gadus morhua), herring (clupea harengus) and flounder (platichtys flesus), in the period 2000-2010. during the study period a marked decline in cesium activity concentration in fish muscle tissue was observed, which reflected changes in radionuclide activity concentration in seawater. no statistically significant temporal trends were determined in chan ...201322863062
distribution and accumulation of elements (as, cu, fe, hg, mn, and zn) in tissues of fish species from different trophic levels in the danube river at the confluence with the sava river (serbia).pikeperch (sander lucioperca), european catfish (silurus glanis), common carp (cyprinus carpio), and gobies (neogobius gymnotrachelus, neogobius melanostomus) were collected from the danube river (belgrade section), and samples of liver, muscle, or whole-body composites (in the case of gobies) were analyzed for as, cu, fe, hg, mn, and zn with inductively coupled plasma optical spectrometry to find out if there was a correlation between accumulation of these elements in predatory and prey species ...201323389858
synergistic impacts by an invasive amphipod and an invasive fish explain native gammarid extinction.worldwide freshwater ecosystems are increasingly affected by invasive alien species. in particular, ponto-caspian gobiid fishes and amphipods are suspected to have pronounced effects on aquatic food webs. however, there is a lack of systematic studies mechanistically testing the potential synergistic effects of invasive species on native fauna. in this study we investigated the interrelations between the invasive amphipod dikerogammarus villosus and the invasive fish species neogobius melanostom ...201627417858
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