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rhizobium meliloti produces a family of sulfated lipooligosaccharides exhibiting different degrees of plant host specificity.we have shown that a rhizobium meliloti strain overexpressing nodulation genes excreted high amounts of a family of n-acylated and 6-o-sulfated n-acetyl-beta-1,4-d-glucosamine penta-, tetra-, and trisaccharide nod factors. either a c(16:2) or a c(16:3) acyl chain is attached to the nonreducing end subunit, whereas the sulfate group is bound to the reducing glucosamine. one of the tetrasaccharides is identical to the previously described nodrm-1 factor. the two pentasaccharides as well as nodrm-1 ...19921729688
analysis of rhizobium meliloti nodulation mutant wl131: novel insertion sequence isrm3 in nodg and altered nodh protein product.nodulation (nod) genes are required for invasion of legumes by rhizobium bacteria. mutant wl131 is a derivative of 102f51 that has a severe nod- phenotype on alfalfa. upon examination of the extended dna region containing host-specific nodulation genes nodfeg and nodh, we found that the nodg gene of wl131 bears a novel insertion sequence, isrm3. complementation studies implied, however, that the phenotype on alfalfa correlated with the nodh locus. we found that nodh in wl131 encodes an altered g ...19911850728
organization, structure and symbiotic function of rhizobium meliloti nodulation genes determining host specificity for alfalfa.in r. meliloti we have identified four nodulation genes determining plant host-range specificity and have designated them hsnabc and d. the genes code for 9.7, 41.7, 26.7, and 28.6 kd proteins, respectively, and are organized into two transcriptional units. mutations in these genes affect nodulation of their natural plant hosts medicago sativa and melilotus albus to different extents and hsnd mutants have an altered host-range. these nod- mutations are not complementable by nodulation genes of o ...19863731273
[obtaining flour and protein concentrate from seeds of melilotus albus. study of the protein quality].the present research was carried out to determine the nutritive quality of the flour and protein concentrate from melilotus albus (white clover) seeds. the flour was studied first. the protein analysis showed methionine to be the first limiting amino acid with a chemical score of 25, with threonine as the second. the biological value obtained was 27. supplementation studies were performed with different levels of methionine and it was found that the 0.3% level resulted in the best net performanc ...19846442553
characterization of rhizobia that nodulate legume species of the genus lespedeza and description of bradyrhizobium yuanmingense sp. nov.legume species belonging to the genus lespedeza are annual or perennial herb or shrub plants that grow in the northern hemisphere. they are known for the formation of root nodules, but little information is available about their microsymbionts. in this study, 58 root-nodule isolates from lespedeza spp., obtained from china and the usa, were characterized using numerical taxonomic analysis of phenotypic features, sds-page analysis of whole-cell proteins, dna-dna hybridization, 16s rrna gene seque ...200212508891
plant bioassays for an in situ monitoring of air near an industrial area and a municipal solid waste: zilina (slovakia).the process of a bioindication of genotoxic effects of complex mixtures on the environment using higher plants is very appropriate and effective. we present the results of an in situ indication of the genotoxic effects of polluted environment near zilina city. for a more complex monitoring we used: the tradescantia micronucleus (trad-mcn) assay, the tradescantia microspore test and an evaluation of the abortivity of the pollen grains of native plant species. we found significant differences in t ...200616617421
[effects of natural and hybrid lectins on the legume-rhizobium interactions].the effects of hybrid lectins--full-sized pea pisum sativum lectin (psl) with the carbohydrate-binding region of white melilot melilotus albus lectin or wild licorice astragalus glycyphyllos lectin substituted for the corresponding psl region (psl/mal and psl/agl, correspondingly)--on the legume-rhizobium symbiosis were studied. the treatment of the rhizobium leguminosarum bv. viciae in the alfalfa (medicago sativa) rhizosphere with psl induced formation of uninfected pseudonodules on its roots, ...200919235514
additions to the rust fungi of argentina.a contribution is made to the rust fungus flora of argentina: puccinia baccharidis-boliviensis, p. cordyceps, p. pucarae, and aecidium hypseocharidicola are described as new species. cionothrix praelonga, frommeëlla mexicana var. mexicana, phakopsora compressa, prospodium peruvianum, puccinia amphiospora, p. chaetochloae, p. liabi, p. pappophori, p. sanguinolenta, p. subaquila, ravenelia havanensis, uredo leonuri, uromyces orbicularis, and u. cnidoscoli are new reports for argentina. new observa ...200221156523
[sinorhizobium meliloti strains screening for efficient bactarization of melilotus albus medik].the data presents about analytical selection of root nodule bacteria of melilotus to obtain bacterial fertilizer under sweet clover, presowing inoculation of it seeds and form a legume-rhizobial effective symbiosis. from natural melilot population a number of new strains had been allocated, inoculation of them was contributed to an increase of height. biomass melilotus albus medik., and nitrogenase activity in comparison to the influence of the existing production strains. the identification of ...201425007439
therapeutic potential of temperate forage legumes: a review.the discovery of bioactive molecules from botanical sources is an expanding field, preferentially oriented to plants having a tradition of use in medicine and providing high yields and availability. temperate forage legumes are fabaceae species that include worldwide-important crops. these plants possess therapeutic virtues that have not only been used in veterinary and folk medicine, but have also attracted the interest of official medicine. we have examined here medicago sativa (alfalfa), trif ...201626507574
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