| agrobacterium bioassay strain for ultrasensitive detection of n-acylhomoserine lactone-type quorum-sensing molecules: detection of autoinducers in mesorhizobium huakuii. | an ultrasensitive bioassay system for the detection of n-acylhomoserine lactones (ahls) was constructed in agrobacterium tumefaciens by using the t7 expression system to overproduce the ahl receptor trar. this strain detected many diverse ahls, some at extremely low concentrations. we used this strain to detect for the first time ahls made by mesorhizobium huakuii, which symbiotically fixes nitrogen in association with the legume astragalus sinicus, a source of green manure throughout eastern as ... | 2003 | 14602662 |
| [screening of target protein interacting with lipid transfer protein ase246 of astragalus sinicus and expression characteristics of corresponding encoding gene]. | ase246 was a novel nodule-specific expressed nodulin gene encoding non-specific lipid transfer protein 1 (nsltp1) of a. sinicus. we screened and identified host plant target protein interacting with ase246, and characterized the expression patterns of target gene under symbiotic and stress conditions. | 2010 | 21141465 |
| [construction of astragalus sinicus ad-cdna library and identification of proteins interact with the leghemoglobin]. | the objectives of this work were (i) to construct a yeast two-hybrid ad-cdna library of astragalus sinicus and provide a fundamental system to screen target proteins involved symbiotic nitrogen fixation, and (ii) to isolate the target proteins interacting with the leghemoglobin. | 2010 | 21365913 |
| the function of three indigenous plasmids in mesorhizobium huakuii 2020 and its symbiotic interaction with sym pjb5ji of rhizobium leguminosarum. | a mesorhizobium huakuii strain 2020, isolated from a rice-growing field in southern china, contains three indigenous plasmids named p2020a, p2020b and p2020c, respectively. the plasmids were deleted via tn5-sacb insertion, and two cured derivatives were obtained. interestingly, the mutant 2020d29 curing of p2020c could significantly enhance the capacity of symbiotic nitrogen fixation. but the mutant 2020d8 curing of p2020b lost the ability to nodulate astragalus sinicus. furthermore, the third p ... | 2008 | 18368313 |
| rna-seq and microarrays analyses reveal global differential transcriptomes of mesorhizobium huakuii 7653r between bacteroids and free-living cells. | mesorhizobium huakuii 7653r occurs either in nitrogen-fixing symbiosis with its host plant, astragalus sinicus, or free-living in the soil. the m. huakuii 7653r genome has recently been sequenced. to better understand the complex biochemical and developmental changes that occur in 7653r during bacteroid development, rna-seq and microarrays were used to investigate the differential transcriptomes of 7653r bacteroids and free-living cells. the two approaches identified several thousand differentia ... | 2014 | 24695521 |
| cloning and identification of lpsh, a novel gene playing a fundamental role in symbiotic nitrogen fixation of mesorhizobium huakuii. | mesorhizobium huakuii 7653r forms a symbiotic relationship with astragalus sinicus to produce nitrogen-fixing root nodules under nitrogen-limiting conditions. by screening its transposon-inserted mutant library, a mutation in the lpsh gene was found to form pseudonodules on a. sinicus. its effect was further confirmed by double-crossover mutant hk242. dna sequence analysis revealed that the lpsh gene encodes a deduced protein of 397 amino acids with a predicted molecular mass of 43.6 kd. lpsh co ... | 2007 | 17486406 |
| arbuscular mycorrhizal fungi mediated uptake of lanthanum in chinese milk vetch (astragalus sinicus l.). | a glasshouse experiment was carried out to study the effect of mycorrhizal formation by gigaspora margarita, glomus intraradices or acaulospora laevis on plant growth and lanthanum (la) uptake of astragalus sinicus l. in soils spiked with la at five levels (0, 1, 5, 10 and 20 mg kg(-1)). la application decreased mycorrhizal infection frequency and activity of fungal succinate dehydrogenase and alkaline phosphatase. increasing la concentrations in soil led to increased la accumulation in tissues ... | 2007 | 17475308 |
| the role of nectar plants in severe outbreaks of armyworm mythimna separata (lepidoptera: noctuidae) in china. | the period from march to mid april, when oriental armyworm mythimna separata (walker) moths migrate from south china to central china over several nights, is an important window of time in annual armyworm population increase. the presence of nectar sources along the pathway of the migratory population is a prerequisite for moths to reach target habitats and lay eggs. using flowering periods and geographic distributions, the major spring nectar plants suitable for m. separata moths were identifie ... | 2006 | 17092356 |
| bioremediation of cadmium contaminated soil using symbiosis between leguminous plant and recombinant rhizobia with the mtl4 and the pcs genes. | cadmium contamination in rice grains is one of the important issues in asian countries. we have developed a novel bio-remediation system based on the symbiosis between leguminous plant and genetically engineered rhizobia. we designed two types of recombinant rhizobia, carrying two genes, synthetic tetrameric metallothionein (mtl4) and cdna encoding phytochelatin synthase from arabidopsis thaliana (atpcs). the mtl4 and atpcs genes were transferred to mesorhizobium huakuii subsp. rengei b3, which ... | 2007 | 16950497 |
| phylogenetic position of mesorhizobium huakuii subsp. rengei, a symbiont of astragalus sinicus cv. japan. | the phylogenetic position of rhizobium huakuii bv. renge, a symbiont of astragalus sinicus cv. japan (renge-sou), was studied. the following phylogenetic approaches were used: restriction fragment length polymorphism (rflp)-polymerase chain reaction (pcr) analysis of a full-length 16s rdna fragment, 16s rdna analysis of the first 300-bp sequence, bacteriophage typing, and amplification of the genomic region by random primer. all the data suggest that strains of r. huakuii bv. renge should be cla ... | 1999 | 16232424 |
| subcellular localization of copper in tolerant and non-tolerant plant. | the ability of elsholtzia splendens naki (e. splendens) to accumulate copper appears to be governed by its high degree of coppertolerance. however, the tolerance mechanism on the physiological basis is unknown. using transmission electron microscope (tem) and energy dispersive analysis of x-rays (edx), the likely location of copper within the cells of the tolerant and non-tolerant was determined. here the role of vacuolar and cell wall compartmentalization in this copper tolerant plant were inve ... | 2005 | 16083123 |
| [interaction between am fungi and rhizobium and effects of flavonoids on it]. | study showed that the biomass and nodule number of astragalus sinicus, am tungal infection rate, and hyphal enzyme activity of alp and sdh were significant influenced by rhizobium, am fungi and flavonoids. rh + amf treatments had obvious differences with control in the biomass and nodule numbers of astragalus sinicus, am fungal infection rate and hyphal enzyme activity; and flavonoid treatments had more obvious differences with rh + amf treatments and control in these aspects, but no obvious dif ... | 2004 | 15669489 |
| mini transposon vector mediated foreign gene expression in mesorhizobium huakuii subsp. rengei. | among the transposable elements, mini-tn5 transposon vector has proven to be of greater utility for insertion mutagenesis of variety of gram negative bacteria. the mini-tn5 vector containing promoter less egfp gene and gentamycin resistant gene was used for the present study. the transposon vector was introduced to m. huakuii from e. coli s17 by conjugation. the conjugants were screened for stable expression of egfp both in free-living and in nodules of astragalus sinicus. the result showed that ... | 2004 | 15511011 |
| curing of symbiotic plasmid of mesorhizobium huakuii subsp. rengei isolated from astragalus sinicus. | astragalus sinicus (chinese milk vetch), a green manure leguminous plant, harbors mesorhizobium huakuii subsp. rengei strain b3 in the root nodules. the visualization of symbiotic plasmid of strain b3 showed the presence of one sym plasmid of about 425 kbp. curing of sym plasmid by temperature and acrydine orange was studied. growing rhizobial cells at high temperature (37 degrees c) or treating the cells with acrydine orange at 50 mg/l eliminated sym plasmid of m. huakuii strain b3, which was c ... | 2003 | 15248496 |
| use of the tobacco feedback-insensitive anthranilate synthase gene (asa2) as a selectable marker for legume hairy root transformation. | the feedback-insensitive anthranilate synthase ( asa2) cdna--isolated from a 5-methyltryptophan (5mt)-resistant tobacco cell line--driven by the camv 35s promoter or 606 bp of the native asa2 promoter, was introduced into the forage legume plant astragalus sinicus or soybean ( glycine max), using agrobacterium rhizogenes strains dc-ar2 or k599, respectively. hairy roots of a. sinicus transformed with 35s-asa2 but not 606- asa2 could be directly selected using 20-75 micro m 5mt. asa2 mrna was exp ... | 2004 | 15168071 |
| enhanced accumulation of cd2+ by a mesorhizobium sp. transformed with a gene from arabidopsis thaliana coding for phytochelatin synthase. | we expressed the arabidopsis thaliana gene for phytochelatin synthase (pcs(at)) in mesorhizobium huakuii subsp. rengei b3, a microsymbiont of astragalus sinicus, a legume used as manure. the pcs(at) gene was expressed under the control of the nifh promoter, which regulates the nodule-specific expression of the nifh gene. the expression of the pcs(at) gene was demonstrated in free-living cells under low-oxygen conditions. phytochelatin synthase (pcs) was expressed and catalyzed the synthesis of p ... | 2003 | 12620871 |
| a novel bioremediation system for heavy metals using the symbiosis between leguminous plant and genetically engineered rhizobia. | a novel plant-bacterial remediation system for heavy metals (hm) was developed by expression of tetrameric human metallothionein (mtl4) in mesorhizobium huakuii subsp. rengei b3, a strain which infects and forms nodules on a green manure, astragalus sinicus. the mtl4 gene was fused to the nifh and nolb promoters, which generated nodule- specific expression of the mtl4 gene. the expression analysis of the mtl4 gene was demonstrated in free-living cells in the presence of cd(2+) and cu(2+), under ... | 2002 | 12385715 |
| dna synthesis and fragmentation in bacteroids during astragalus sinicus root nodule development. | histo- and cytochemical techniques were used to study the dna replication and fragmentation patterns in bacteroids formed by mesorhizobium huakuii subsp. rengei in nodules of astragalus sinicus. dna replication was detected by the incorporation of 5-bromo deoxy-uridine. signals denoting dna synthesis were observed in plant nuclei within the nodule meristem and in bacteroids near the meristem. the tunel (tdt-mediated dutp nick-end labeling) assay was used to measure dna fragmentation. in nutrient ... | 2001 | 11330661 |
| the involvement of a cysteine proteinase in the nodule development in chinese milk vetch infected with mesorhizobium huakuii subsp. rengei. | cys proteinases play important roles in plant cell development and senescence. a cdna, asnodf32, obtained by differential screening of a nodule cdna library of the leguminous plant chinese milk vetch (astragalus sinicus), represents a nodule-specific cys proteinase similar to that reported for the actinorhizal alnus glutinosa-flankia symbiosis. a characteristic feature of this proteinase is the presence of a putative vacuolar targetting signal, lqda, within its propeptide. expression of the asno ... | 2000 | 11080286 |
| symbiosis of astragalus cicer with its microsymbionts: partial nodc gene sequence, host plant specificity, and root nodule structure. | astragalus cicer (cicer milkvetch) nodule bacteria were investigated for host plant specificity and partial nodc gene sequences, whilst their native host was studied for the microscopic structure of root nodules. the strains under investigation formed nodules not only on the original host but also on astragalus glycyphyllos, astragalus sinicus, lotus corniculatus, and phaseolus vulgaris. the nodules induced on the cicer milkvetch were classified as indeterminate and characterized by apical, pers ... | 2000 | 11016697 |
| increasing tryptophan synthesis in a forage legume astragalus sinicus by expressing the tobacco feedback-insensitive anthranilate synthase (asa2) gene. | a cdna clone that encodes a feedback-insensitive anthranilate synthase (as), asa2, isolated from a 5-methyl-tryptophan (trp) (5mt)-resistant tobacco cell line under the control of the constitutive cauliflower mosaic virus 35s promoter, was introduced into the forage legume astragalus sinicus by agrobacterium rhizogenes with kanamycin selection. the 35s-asa2 gene was expressed constitutively as demonstrated by northern-blot hybridization analyses and the presence of feedback-insensitive as. hairy ... | 2000 | 10889256 |
| the common nodulation genes of astragalus sinicus rhizobia are conserved despite chromosomal diversity. | the nodulation genes of mesorhizobium sp. (astragalus sinicus) strain 7653r were cloned by functional complementation of sinorhizobium meliloti nod mutants. the common nod genes, nodd, noda, and nodbc, were identified by heterologous hybridization and sequence analysis. the noda gene was found to be separated from nodbc by approximately 22 kb and was divergently transcribed. the 2. 0-kb noddbc region was amplified by pcr from 24 rhizobial strains nodulating a. sinicus, which represented differen ... | 2000 | 10877796 |
| structure of the mesorhizobium huakuii and rhizobium galegae nod factors: a cluster of phylogenetically related legumes are nodulated by rhizobia producing nod factors with alpha,beta-unsaturated n-acyl substitutions. | rhizobia are symbiotic bacteria that synthesize lipochitooligosaccharide nod factors (nfs), which act as signal molecules in the nodulation of specific legume hosts. based on the structure of their n-acyl chain, nfs can be classified into two categories: (i) those that are acylated with fatty acids from the general lipid metabolism; and (ii) those (= alphau-nfs) that are acylated by specific alpha,beta-unsaturated fatty acids (containing carbonyl-conjugated unsaturation(s)). previous work has de ... | 1999 | 10564467 |
| Identification of a novel gene for biosynthesis of a bacteroid-specific electron carrier menaquinone. | Ubiquinone (UQ) has been considered as an electron mediator in electron transfer that generates ATP in Rhizobium under both free-living and symbiosis conditions. When mutated, the dmtH gene has a symbiotic phenotype of forming ineffective nodules on Astragalus sinicus. The gene was isolated from a Mesorhizobium huakuii 7653R transposon-inserted mutant library. The DNA sequence and conserved protein domain analyses revealed that dmtH encodes demethylmenaquinone (DMK) methyltransferase, which cata ... | 2011 | 22194970 |
| structural studies of the o-specific polysaccharide from the lipopolysaccharide of mesorhizobium huakuii strain s-52, the symbiotic partner of astragalus sinicus. | the o-specific polysaccharide (ops) obtained by mild-acid degradation of the lipopolysaccharide isolated from mesorhizobium huakuii strain s-52 was studied by sugar and ethylation analyses along with (1)h and (13)c nmr spectroscopy. it was concluded that the ops was composed of trisaccharide repeating units containing two residues of 6-deoxy-l-talose (6dtal) and one l-rhamnose (rha), whose sequence in the ops was determined by noesy and hmbc experiments. the minor 3-o-acetylation (about 10%) of ... | 2011 | 21486667 |
| the differential behavior of arbuscular mycrorrhizal fungi in interaction with astragalus sinicus l. under salt stress. | three arbuscular mycorrhizal (am) fungi (glomus mosseae, glomus claroideum, and glomus intraradices) were compared for their root colonizing ability and activity in the root of astragalus sinicus l. under salt-stressed soil conditions. mycorrhizal formation, activity of fungal succinate dehydrogenase, and alkaline phosphatase, as well as plant biomass, were evaluated after 7 weeks of plant growth. increasing the concentration of nacl in soil generally decreased the dry weight of shoots and roots ... | 2011 | 20393756 |
| baca is indispensable for successful mesorhizobium-astragalus symbiosis. | a homologue of sinorhizobium meliloti baca was isolated from mesorhizobium huakuii 7653r, which is capable of fixing atmospheric nitrogen in symbiotic association with leguminous astragalus sinicus (chinese milk vetch). inactivation of the baca gene abolished the ability of m. huakuii 7653r to establish a successful symbiosis with its host plant. simultaneously, compared with wild-type m. huakuii 7653r, the baca mutant was more sensitive to cell envelope-disrupting agents (acidic solution, ethan ... | 2009 | 19352651 |
| a nodule-specific plant cysteine proteinase, asnodf32, is involved in nodule senescence and nitrogen fixation activity of the green manure legume astragalus sinicus. | asnodf32, encoding a nodule-specific cysteine proteinase in astragalus sinicus, is probably involved in nodule senescence. to obtain direct evidence of its role in nodule senescence, agrobacterium rhizogenes-mediated rna interference was applied to a. sinicus hairy roots. real-time qrt-pcr was used to estimate the efficiency of suppression. the senescent phenotype of transgenic nodules was examined with paraffin-embedded slides, tunel (tdt-mediated dutp nick-end labeling) assay, and transmission ... | 2008 | 18643938 |
| characterization of astragalus sinicus rhizobia by restriction fragment length polymorphism analysis of chromosomal and nodulation genes regions. | two hundred and four isolates of rhizobia were sampled from root nodules of astragalus sinicus grown in rice fields of six southern provinces of china. genotypic diversity was determined by southern hybridization using noddbc genes as a probe, restriction fragment length polymorphism (rflp) analysis of pcr-amplified 16s-23s rdna intergenic spacers (igs), and plasmid profile. our results show that rhizobia associated with a. sinicus were very diverse, and 10 genotypes were resolved within the pre ... | 1999 | 10525842 |
| asnodc22, a novel nodulin gene of astragalus sinicus, encodes a protein that localizes along the cell wall of bacteria-infected cells in a nodule. | a novel nodulin gene, asnodc22, was isolated from astragalus sinicus cv. japan (renge-sou) by differential screening. the transcript of asnodc22 was nodule specific, and its size was approximately 0.8 knt. asnodc22 encoded an unknown orf containing a putative signal peptide and a pair of similar hepta-peptides (csaqvsc, csaqlsc). for immunological study, antiserum against asnodc22 protein was prepared in mice. by immunoblot analysis, the antiserum detected a nodule specific band at approximately ... | 1998 | 9787458 |
| triterpene glycosides from the seeds of astragalus sinicus l. | from the seeds of astragalus sinicus l. (leguminosae), seven triterpene glycosides were isolated and identified as soyasaponin i-iii methyl esters (1-3) which were treated with ch2n2 during the separation procedure, soyasaponin iv (4), soyasapogenol b 3-o-beta-d-glucuronopyranoside (5), 3-o-alpha-l-rhamnopyranosyl(1-->2)-beta-d-xylopyranosyl(1-->2)-beta-d- glucuronopyranosyl 3 beta, 22 beta, 24-trihydroxy-11-oxoolean-12-ene (6), whose sapogenol (8) was obtained by enzymatic hydrolysis using glyc ... | 1992 | 1294336 |
| promotion of metal accumulation in nodule of astragalus sinicus by the expression of the iron-regulated transporter gene in mesorhizobium huakuii subsp. rengei b3. | toxic metal contamination in agricultural fields is an important worldwide problem. in previous studies, we developed a bioremediation system based on the symbiosis between astragalus sinicus and the recombinant rhizobium, mesorhizobium huakuii subsp. rengei b3 developed by overexpressing a synthetic tetrameric metallothionein gene (mtl4) and cdna encoding the phytochelatin synthase from arabidopsis thaliana (atpcs). to promote the transport of metals into the nodules of the rhizobium and the ac ... | 2008 | 18640604 |
| a nodule-specific lipid transfer protein ase246 participates in transport of plant-synthesized lipids to symbiosome membrane and is essential for nodule organogenesis in chinese milk vetch. | rhizobia in legume root nodules fix nitrogen in symbiosomes, organelle-like structures in which a membrane from the host plant surrounds the symbiotic bacteria. however, the components that transport plant-synthesized lipids to the symbiosome membrane remain unknown. this study identified and functionally characterized the chinese milk vetch (astragalus sinicus) lipid transfer protein ase246, which is specifically expressed in nodules. it was found that ase246 can bind lipids in vitro. more impo ... | 2013 | 24367021 |
| a jaz protein in astragalus sinicus interacts with a leghemoglobin through the tify domain and is involved in nodule development and nitrogen fixation. | leghemoglobins (lbs) play an important role in legumes-rhizobia symbiosis. lbs bind o2 and protect nitrogenase activity from damage by o2 in nodules, therefore, they are regarded as a marker of active nitrogen fixation in nodules. additionally, lbs are involved in the nitric oxide (no) signaling pathway, acting as a no scavenger during nodule development and nitrogen fixation. however, regulators responsible for lb expression and modulation of lb activity have not been characterized. in our prev ... | 2015 | 26460857 |
| rhizobia with 16s rrna and nifh similar to mesorhizobium huakuii but novel reca, glnii, noda and nodc genes are symbionts of new zealand carmichaelinae. | new zealand became geographically isolated about 80 million years ago and this separation gave rise to a unique native flora including four genera of legume, carmichaelia, clianthus and montigena in the carmichaelinae clade, tribe galegeae, and sophora, tribe sophoreae, sub-family papilionoideae. ten bacterial strains isolated from nz carmichaelinae growing in natural ecosystems grouped close to the mesorhizobium huakuii type strain in relation to their 16s rrna and nifh gene sequences. however, ... | 2012 | 23118889 |
| mesorhizobium qingshengii sp. nov., isolated from effective nodules of astragalus sinicus. | in a study on the diversity of rhizobia isolated from root nodules of astragalus sinicus, five strains showed identical 16s rrna gene sequences. they were related most closely to the type strains of mesorhizobium loti, mesorhizobium shangrilense, mesorhizobium ciceri and mesorhizobium australicum, with sequence similarities of 99.6-99.8%. a polyphasic approach, including 16s-23s intergenic spacer (igs) rflp, comparative sequence analysis of 16s rrna, atpd, glnii and reca genes, dna-dna hybridiza ... | 2013 | 23041644 |
| functional analysis of the novel mycorrhiza-specific phosphate transporter aspt1 and pht1 family from astragalus sinicus during the arbuscular mycorrhizal symbiosis. | arbuscular mycorrhizas contribute significantly to inorganic phosphate (pi) uptake in plants. gene networks involved in the regulation and function of the pht1 family transporters in legume species during am symbiosis are not fully understood. in order to characterize the six distinct members of pht1 transporters in mycorrhizal astragalus sinicus, we combined cellular localization, heterologous functional expression in yeast with expression/subcellular localization studies and reverse genetics a ... | 2013 | 23442117 |
| comparative enzymology of the adenosine triphosphate sulphurylases from leaf tissue of selenium-accumulator and non-accumulator plants. | 1. atp sulphurylases were partially purified (20-40-fold) from leaf tissue of astragalus bisulcatus, astragalus racemosus (selenium-accumulator species) and astragalus hamosus and astragalus sinicus (non-accumulator species). activity was measured by sulphate-dependent pp(i)-atp exchange. the enzymes were separated from pyrophosphatase and adenosine triphosphatase activities. the properties of the astragalus atp sulphurylases were similar to the spinach enzyme. 2. the atp sulphurylases from both ... | 1974 | 4377098 |
| rhizobium arenae sp. nov., isolated from the sand of desert mu us, china. | a gram-strain-negative, rod-shaped, motile bacterium, designated mim27t, was isolated from the sand of desert mu us, china. the strain could grow at 4-45 °c (optimum, 37 °c), at ph 6.5-9.0 (optimum, 8.0) and in the presence of 0-3 % (w/v) nacl (optimum, 0 %). phylogenetic analysis on 16s rrna gene sequence demonstrated that the strain was belonged to the genus rhizobium, with the highest similarity (96.5 %) to rhizobium pakistanensis bn-19t. analysis of nitrogen fixation gene nifh and three hous ... | 2017 | 28126039 |
| isolation of the pcb-degrading bacteria mesorhizobium sp. zy1 and its combined remediation with astragalus sinicus l. for contaminated soil. | a bacterial strain zy1 capable of utilizing pcbs as its carbon source was isolated from the root nodules of chinese milk vetch (astragalus sinicus l.). the strain was identified as mesorhizobium sp. according to its physiological-biochemical properties and the analysis of its 16s rrna gene sequence. when the initial od600 was 0.15, 62.7% of 15 mg l(-1) 3,3',4,4'-tcb in a liquid culture was degraded by mesorhizobium sp. zy1 within 10 days. mesorhizobium sp. zy1 also greatly increased the biotrans ... | 2016 | 26292091 |
| a purple acid phosphatase plays a role in nodule formation and nitrogen fixation in astragalus sinicus. | the asppd1 gene from astragalus sinicus encodes a purple acid phosphatase. to address the functions of asppd1 in legume-rhizobium symbiosis, its expression patterns, enzyme activity, subcellular localization, and phenotypes associated with its over-expression and rna interference (rnai) were investigated. the expression of asppd1 was up-regulated in roots and nodules after inoculation with rhizobia. phosphate starvation reduced the levels of asppd1 transcripts in roots while increased those leve ... | 2015 | 26105827 |
| rhizobium oryzicola sp. nov., potential plant-growth-promoting endophytic bacteria isolated from rice roots. | bacterial strains zyy136(t) and zyy9 were isolated from surface-sterilized rice roots from a long-term experiment of rice-rice--astragalus sinicus rotation. the 16s rrna gene sequences of strains zyy136(t) and zyy9 showed the highest similarity, of 97.0%, to rhizobium tarimense pl-41(t). sequence analysis of the housekeeping genes reca, thrc and atpd clearly differentiated the isolates from currently described species of the genus rhizobium. the dna-dna relatedness value between zyy136(t) and zy ... | 2015 | 26016492 |
| host-plant dependent population genetics of the invading weevil hypera postica. | population genetics of invading pests can be informative for understanding their ecology. in this study, we investigated population genetics of the invasive alfalfa weevil hypera postica in fukuoka prefecture, japan. we analyzed mitochondrial trnaleu-coii, nuclear ef-1α gene fragments, and wolbachia infection in relation to three leguminous host plants: vicia angustifolia, vicia villosa, and a new host astragalus sinicus cultivated as a honey source and green manure crop. a parsimony network gen ... | 2015 | 25336385 |
| effects of winter cover crops straws incorporation on ch4 and n2o emission from double-cropping paddy fields in southern china. | residue management in cropping systems is believed to improve soil quality. however, the effects of residue management on methane (ch4) and nitrous oxide (n2o) emissions from paddy field in southern china have not been well researched. the emissions of ch4 and n2o were investigated in double cropping rice (oryza sativa l.) systems with straw returning of different winter cover crops by using the static chamber-gas chromatography technique. a randomized block experiment with three replications wa ... | 2014 | 25271923 |
| evaluating oxidation-reduction properties of dissolved organic matter from chinese milk vetch (astragalus sinicus l.): a comprehensive multi-parametric study. | green manuring is a common practice in replenishment of soil organic matter and nutrients in rice paddy field. owing to the complex interplay of multiple factors, the oxidation--reduction (redox) properties of dissolved organic matter (dom) from green manure crops are presently not fully understood. in this study, a variety of surrogate parameters were used to evaluate the redox capacity and redox state of dom derived from chinese milk vetch (cmv, astragalus sinicus l.) via microbial decompositi ... | 2014 | 24956785 |
| effects of winter cover crops residue returning on soil enzyme activities and soil microbial community in double-cropping rice fields. | residue management in cropping systems is useful to improve soil quality. however, the studies on the effects of residue management on the enzyme activities and microbial community of soils in south china are few. therefore, the effects of incorporating winter cover crop residue with a double-cropping rice (oryza sativa l.) system on soil enzyme activities and microbial community in southern china fields were studied. the experiment has conducted at the experimental station of the institute of s ... | 2014 | 24956152 |
| anti-inflammatory activity of compounds isolated from astragalus sinicus l. in cytokine-induced keratinocytes and skin. | inflammation is a part of the complex biological responses of a tissue to injury that protect the organ by removing injurious stimuli and initiating the healing process, and is considered as a mechanism of innate immunity. to identify biologically active compounds against pathogenic inflammatory and immune responses, we fractionated water, aqueous methanol and n-hexane layers from nine kinds of leguminosae and examined anti-inflammatory activity of the fractions in human keratinocytes and mouse ... | 2014 | 24651533 |
| a novel nodule-enhanced gene encoding a putative universal stress protein from astragalus sinicus. | a nodule-enhanced gene, asd243, was identified from infected roots of astragalus sinicus using suppressive subtractive hybridization (ssh). it encodes a 20-kd protein related to the bacterial universal stress protein family (usp). sequence analysis showed that asd243 is highly similar to the bacterial mj0577-type of atp-binding usp proteins, which have been proposed to function as a molecular switch. expression analyses revealed that asd243 was transcribed in all plant organs, and progressively ... | 2007 | 16884822 |
| thirteen nodule-specific or nodule-enhanced genes encoding products homologous to cysteine cluster proteins or plant lipid transfer proteins are identified in astragalus sinicus l. by suppressive subtractive hybridization. | thirteen nodule-specific or nodule-enhanced genes have been revealed by suppressive subtractive hybridization (ssh) with two mrna populations of infected and uninfected control roots of astragalus sinicus. eleven of them encode small polypeptides showing homology to cysteine cluster proteins (ccps) that contain a putative signal peptide and conserved cysteine residues. among these ccp-like genes, asg257 codes for a homologue of the defensin 2 family and asd255 contains a scorpion toxin-like doma ... | 2006 | 16831842 |
| molecular cloning and characterization of two cdnas encoding asparagine synthetase from astragalus sinicus nodules. | two cdnas that encode asparagine synthetase were cloned from root nodules of astragalus sinicus cv. japan (renge-sou). the expression of the transcripts was nodule-enhanced. the expression of both genes was reduced in nodules when (nh4)2so4 was added to the culture medium. this is the first report of the inhibition of asparagine synthetase gene expression by ammonium in root nodules. | 2000 | 16232798 |
| effects of gold banded lily (lilium auratum lindl) or chinese milk vetch (astragalus sinicus l) on spontaneous mammary tumourigenesis in shn mice. | some guanidino compounds have been found to inhibit spontaneous mammary tumourigenesis in mice. in the present study, the effects of chronic treatment with gold banded lily (lilium auratum lindi) or chinese milk vetch (astragalus sinicus l), which contains l-arginine or l-canavanine, on spontaneous mammary tumourigenesis were examined in shn mice. free access to drinking water containing hot water extract of each natural product at a concentration of 0.5% significantly inhibited the development, ... | 2004 | 11724289 |
| functional centromeres in astragalus sinicus include a compact centromere-specific histone h3 and a 20-bp tandem repeat. | the centromere plays an essential role for proper chromosome segregation during cell division and usually harbors long arrays of tandem repeated satellite dna sequences. although this function is conserved among eukaryotes, the sequences of centromeric dna repeats are variable. most of our understanding of functional centromeres, which are defined by localization of a centromere-specific histone h3 (cenh3) protein, comes from model organisms. the components of the functional centromere in legume ... | 2011 | 22065151 |
| the effect of cd on mycorrhizal development and enzyme activity of glomus mosseae and glomus intraradices in astragalus sinicus l. | the influence of cadmium (cd) on the root colonization of astragalus by two arbuscular mycorrhizal fungi (amf) was investigated. astragalus sinicus l. grown in the soil in the presence of four levels of cd was inoculated individually with glomus mosseae, glomus intraradices, or by a mixed inoculum of these two fungi. histochemical vital stains were used to measure the efficiency and function of the mycorrhizal symbiosis after 7weeks. nested pcr with alp-active stained roots was applied to monito ... | 2009 | 19232430 |
| [influence of double rice cropping system innovation on paddy soil profile form and soil characteristics]. | field experiments were conducted on the double rice cropping paddy field in red soil area to evaluate the influence of cropping system innovation on soil profile form and related soil characteristics. four cropping systems of rice-rice-chinese milkvetch (astragalus sinicus linn.), forage, paddy-upland rotation, and upland were substituted for the double rice cropping system. the results indicated that compared with those under double rice cropping system, the thickness of cultivated horizon unde ... | 2008 | 18655589 |
| the symbiosis phenotype and expression patterns of five nodule-specific genes of astragalus sinicus under ammonium and salt stress conditions. | in previous works, we isolated 14 nodule-specific or nodule-enhanced genes from astragalus sinicus by suppressive subtractive hybridization. in this study, we have further identified the expression patterns of five nodule-specific genes of a. sinicus under salt and ammonium stress. transcription levels of genes tested were quantified by quantitative fluorescence real-time rt-pcr. results showed that: (1) about 80 mm nacl and all stress treatments containing (nh(4))(2)so(4) significantly inhibite ... | 2007 | 17415569 |