Publications

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the noguchi-adler phenomenon: an ultrastructural study of the effects of homologous antiserum on the growth of promastigotes of leishmania braziliensis braziliensis and leishmania hertigi hertigi.a light and electron microscope study was made of the agglutinated bodies formed on growing promastigotes of a leishmania b braziliensis and a l. h. hertigi strain in their homologous antisera to determine the role of leishmanial excreted factor (ef) in the noguchi-adler phenomenon, since the l. b. braziliensis strain produced a demonstrable ef, whereas the l. h. hertigi strain did not. promastigotes of l. b. braziliensis, when grown in high concentrations of homologous antiserum, formed noguchi ...1979395912
studies on the ultrastructure, virus-like particles and infectivity of leishmania hertigi.five strains of leishmania hertigi hertigi isolated in panama and three strains of l. hertigi deanei isolated in brazil were studied. ultrastructural examination of promastigotes grown in culture showed virus-like particles (vlps), 55--60 nm diameter, in the cytoplasm of all strains. the vlps were normally either organized in paracrystalline clusters or associated with induced tubules. in some cases the vlps were associated with dense vesicular bodies. mitochondria with which the vlps were assoc ...1979496473
the morphological, biochemical and serological characterization of strains of leishmania hertigi from panama and brazil and their differentiation. 1978655741
finding of leishmania hertigi in the costa rican porcupine. 1977915623
pathogenicity of crithidia fasciculata in the haemocoele of glossina.the mosquito flagellate crithidia fasciculata produces intense haemocoelic infections following intrahaemocoelic inoculation into 5 species of glossina--g. austeni, g. fuscipes fuscipes, g. morsitans morsitans, g. palpalis gambiensis and g. tachinoides. all glossina inoculated with c. fasciculata died between days 4 and 9. neither trypanosoma brucei procyclics nor leishmania hertigi promastigotes similarly inoculated into glossina species, at the same dose, multiplied within the haemocoele and n ...19872884835
investigation of virus-like particles in leishmania hertigi.the promastigote forms of an isolate of leishmania hertigi contain large numbers of virus-like particles (vlps) in paracrystalline arrays. the vlps are not present in the amastigote form and it was not possible to purify them from large amounts of the promastigotes.19873683213
leishmania hertigi sp. n., from the tropical porcupine, coendou rothschildi thomas. 19715090970
naturally occurring agglutinins against trypanosomatid flagellates in the haemolymph of insects.in vitro studies of the behaviour of the trypanosomatid flagellates trypanosoma brucei and leishmania hertigi in the presence of cell-free haemolymph of locusts, schistocerca gregaria and cockroaches, periplaneta americana revealed the presence of parasite agglutinins. the range of normal values of agglutination titres was 2(-4) to 2(-13). physico-chemical treatment of haemolymph indicated that these agglutinins are protein or glycoprotein in nature and are only partially affected by heat treatm ...19846096791
anti-trypanosomal factor in the haemolymph of glossina.the motility of cultured procyclic forms of trypanosoma brucei brucei antat serodeme derived from eatro 1125 was greatly reduced when incubated in vitro with the haemolymph of glossina morsitans morsitans at dilutions as low as 1:512 after incubation periods of 1-2 h at 27 degrees c. this effect was demonstrated in the haemolymph of male and female, teneral and non-teneral g.m. morsitans but was abolished by heat inactivation. a significant reduction in the motility of cultured forms of trypanos ...19826187195
haemagglutinins and parasite agglutinins in haemolymph and gut of glossina.agglutinating activity was found in the haemolymph and in extracts of midgut and hindgut of glossina austeni against calf, guinea pig and chicken erythrocytes for the first time. trypanosoma brucei procyclic forms were also agglutinated by midgut and hindgut extracts, but not by haemolymph. the 3 fractions tested failed to react with erythrocytes of four other vertebrates and other trypanosomatids (leishmania hertigi and crithidia fasciculata) examined. the reciprocal titre of agglutinating acti ...19846495384
[vaccination with leishmania hertigi against the subsequent infection with leishmania mexicana and leishmania braziliensis in hamsters]. 19806782641
some methods for the enzymic characterization of latin-american leishmania with particular reference to leishmania mexicana amazonensis and subspecies of leishmania hertigi.30 brazilian stocks of leishmania mexicana amazonensis and 13 stocks of subspecies of leishmania hertigi were characterized by starch-gel electrophoresis, using 18 enzymes selected from a total of 36 investigated. l. m. amazonensis was separable from subspecies of l. hertigi by enzymic profiles of 11 enzymes. the l. m. amazonensis stocks, which were from a wide range of hosts in a large geographical area, were enzymically extremely homogeneous, and could only be subdivided on two enzymes; sub-gr ...19807385303
the leishmania hertigi (kinetoplastida; trypanosomatidae) complex and the lizard leishmania: their classification and evidence for a neotropical origin of the leishmania-endotrypanum clade.the relationships of the leishmania hertigi complex and the lizard leishmania species to the main groups of mammalian leishmania and endotrypanum parasites were examined. restriction fragment length polymorphisms and sequences of small subunit ribosomal rna genes and hybridization studies of kinetoplast dna indicated that the l. hertigi complex was more closely related to the genus endotrypanum than to the genus leishmania. the lizard leishmania species were found to be at the crown of the leish ...19979304821
evolution of the genus leishmania revealed by comparison of dna and rna polymerase gene sequences.previous hypotheses of leishmania evolution are undermined by limitations in the phylogenetic reconstruction method employed or due to the omission of key parasites. in this experiment, sequences of the gene encoding the dna polymerase alpha catalytic polypeptide (pola) were analysed phylogenetically in combination with those encoding the rna polymerase ii largest subunit gene (rpoiils) to infer a comprehensive phylogeny of leishmania. nineteen species of parasites were studied, comprising repre ...19979364962
phylogenetic relationships of leishmania species based on trypanosomatid barcode (ssu rdna) and ggapdh genes: taxonomic revision of leishmania (l.) infantum chagasi in south america.phylogenetic studies on trypanosomatid barcode using v7v8 ssu rrna and ggapdh gene sequences have provided support for redefining some trypanosomatid species and positioning new isolates. the genus leishmania is a slow evolving monophyletic group and including important human pathogens. the phylogenetic relationships of this genus have been determined by the natural history of its vertebrate hosts, vector specificity, clinical manifestations, geographical distribution and molecular approaches us ...201424747606
leishmania (leishmania) hertigi in a porcupine (coendou sp.) found in brasília, federal district, brazil.studies report the occurrence of leishmania (leishmania) hertigi in northern states of brazil. in the present investigation, we describe the isolation of l. (l.) hertigi from a porcupine (coendou sp.) found in brasília, federal district, center-west region of brazil. during a study on canine visceral leishmaniasis conducted in the city of brasília, federal district, a porcupine was found dead on a public road. the animal was identified and fragments of intact skin and spleen were collected for i ...201323538497
aspects on the ecology of phlebotomine sand flies and natural infection by leishmania hertigi in the southeastern amazon basin of brazil.the medical and veterinary importance of sand flies (diptera: psychodidae) follow as a result of some species ability to transmit the zoonotic protozoa of the genus leishmania. of all municipalities in the state of mato grosso, sinop ranks first in reported cases of american cutaneous leishmaniasis (acl). sinop urban zone encompasses three permanent forest preservation areas (apps) that provide refuge for insects and other vertebrate hosts. we assessed ecological parameters and investigated the ...201828964769
in vitro anti-leishmania activity of tetracyclic iridoids from morinda lucida, benth.leishmaniasis is an infectious disease transmitted by the sand fly. it is caused by over 20 different species of leishmania and has affected over 14 million people worldwide. one of the main forms of control of leishmaniasis is chemotherapy, but this is limited by the high cost and/or toxicity of available drugs. we previously found three novel compounds with an iridoid tetracyclic skeleton to have activity against trypanosome parasites. in this study, we determined the activity of the three ant ...201627536194
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