| rio mamore virus: genetic characterization of a newly recognized hantavirus of the pygmy rice rat, oligoryzomys microtis, from bolivia. | human hantavirus disease occurs throughout much of south america. the rodent hosts and the specific etiologic agent(s) are largely unknown, but many reported cases occurred within the habitation ranges of oryzomine rodents (rice rats). we have identified a genetically novel hantavirus (rio mamore virus [rm]) of the pygmy rice rat oligoryzomys microtis in bolivia. the complete sequence of the small (s) genome and the partial sequence of the medium (m) genome are described. this virus is closely r ... | 1997 | 9311652 |
| isolation and genetic characterization of a hantavirus (bunyaviridae: hantavirus) from a rodent, oligoryzomys microtis (muridae), collected in northeastern peru. | this paper describes the isolation and partial genetic characterization of a hantavirus from a pygmy rice rat, oligoryzomys microtis, collected within the urban area of iquitos, loreto department, peru. the virus, designated htn-007, exhibited the highest degree of genetic similarity to rio mamore virus, which was originally described from the same rodent species in eastern bolivia. comparison of small and medium segment nucleotide sequence data from htn-007 and rio mamore virus revealed 87% and ... | 1999 | 10432063 |
| genetic investigation of novel hantaviruses causing fatal hps in brazil. | although hantavirus pulmonary syndrome (hps) was discovered in north america in 1993, more recent investigations have shown that the disease is a much larger problem in south america, where a greater number of cases and hps-associated viruses have now been detected. here we describe the genetic investigation of three fatal hps cases from brazil, including a 1995 case in castelo dos sonhos (cas) in the state of mato grosso and two 1996 cases in the counties of araraquara (ara) and franca (fra), i ... | 1999 | 10534737 |
| analysis of hantavirus genetic diversity in argentina: s segment-derived phylogeny. | nucleotide sequences were determined for the complete s genome segments of the six distinct hantavirus genotypes from argentina and for two cell culture-isolated andes virus strains from chile. phylogenetic analysis indicates that, although divergent from each other, all argentinian hantavirus genotypes group together and form a novel phylogenetic clade with the andes virus. the previously characterized south american hantaviruses laguna negra virus and rio mamore virus make up another clade tha ... | 2002 | 11907216 |
| hantavirus pulmonary syndrome in central bolivia: relationships between reservoir hosts, habitats, and viral genotypes. | in august 2002, two cases of hantavirus pulmonary syndrome (hps) were confirmed in mineros and concepcion, within the santa cruz department of bolivia. extensive alteration of the native ecosystem, from dense forest to pasture or sugarcane, had occurred in both regions. an ecologic assessment of reservoir species associated with the human disease identified a single hantavirus antibody-positive oligoryzomys microtis from mineros and three hantavirus antibody-positive calomys callosus from concep ... | 2005 | 15728866 |
| newly recognized hantaviruses associated with hantavirus pulmonary syndrome in northern brazil: partial genetic characterization of viruses and serologic implication of likely reservoirs. | following the occurrence of the first laboratory-confirmed cases of hantavirus pulmonary syndrome (hps) in maranhao state, brazil, rodents were trapped and rodent materials screened by elisa for antibodies to sin nombre and andes hantaviruses. antibody-positive samples were tested by rt-pcr, amplified products were sequenced, and phylogenetic trees were constructed for comparison with known hantaviruses. from 104 rodent blood samples collected (40 bolomys lasiurus, 52 holochilus sciureus, 12 oli ... | 2005 | 15815145 |
| geographical range of rio mamoré virus (family bunyaviridae, genus hantavirus) in association with the small-eared pygmy rice rat (oligoryzomys microtis). | hantavirus htn.007 was originally isolated from a small-eared pygmy rice rat (oligoryzomys microtis) captured in northeastern peru. the results of analyses of nucleotide and amino acid sequence data in this study indicated that htn.007 is a strain of rio mamoré virus (riomv) which is enzootic in small-eared pygmy rice rat populations in bolivia. as such, the results of this study extend our knowledge of the geographical range of riomv and support the notion that the small-eared pygmy rice rat is ... | 2010 | 20687859 |
| serologic evidence for human hantavirus infection in peru. | while human illness associated with hantavirus infection has been documented in many countries of south america, evidence for hantavirus transmission in peru has been limited to the isolation of rio mamore virus from a pigmy mouse rat (oligoryzomys microtis) in the amazon city of iquitos. to address the possibility of human hantavirus exposure in the region, we screened febrile patients reporting to health clinics in iquitos from 2007 to 2010 for serological evidence of recent hantavirus infecti ... | 2012 | 22616720 |
| detection of different south american hantaviruses. | hantaviruses are the etiologic agents of hemorrhagic fever with renal syndrome (hfrs) in old world, and hantavirus pulmonary syndrome (hps)/hantavirus cardiopulmonary syndrome (hcps), in the new world. serological methods are the most common approach used for laboratory diagnosis of hcps, however theses methods do not allow the characterization of viral genotypes. the polymerase chain reaction (pcr) has been extensively used for diagnosis of viral infections, including those caused by hantavirus ... | 2015 | 26220480 |
| development of a novel plaque reduction neutralisation test for hantavirus infection. | in the americas, hantaviruses cause severe cardiopulmonary syndrome (hcps) with a high fatality rate. hantavirus infection is commonly diagnosed using serologic techniques and reverse transcription-polymerase chain reaction. this paper presents a novel plaque reduction neutralisation test (prnt) for detecting antibodies to brazilian hantavirus. using prnt, plaque detection was enhanced by adding 0.6% of dimethyl sulfoxide into the overlay culture medium of the infected cells. this procedure faci ... | 2015 | 26132430 |
| development of a one-step sybr green i real-time rt-pcr assay for the detection and quantitation of araraquara and rio mamore hantavirus. | hantaviruses are members of the family bunyaviridae and are an emerging cause of disease worldwide with high lethality in the americas. in brazil, the diagnosis for hantaviruses is based on immunologic techniques associated with conventional rt-pcr. a novel one-step sybr green real-time rt-pcr was developed for the detection and quantitation of araraquara (arav) and rio mamore hantavirus (riomv). the detection limit of assay was 10 copies/μl of rna in vitro transcribed of segment s. the specific ... | 2013 | 24056673 |
| hantavirus pulmonary syndrome in santa cruz, bolivia: outbreak investigation and antibody prevalence study. | we report the results of an investigation of a small outbreak of hantavirus pulmonary syndrome in 2002 in the department of santa cruz, bolivia, where the disease had not previously been reported. two cases were initially reported. the first case was a physician infected with laguna negra virus during a weekend visit to his ranch. four other persons living on the ranch were igm antibody-positive, two of whom were symptomatic for mild hantavirus pulmonary syndrome. the second case was a migrant s ... | 2012 | 23094116 |
| pneumonitis in syrian golden hamsters (mesocricetus auratus) infected with rio mamoré virus (family bunyaviridae, genus hantavirus). | rio mamoré virus is an etiological agent of hantavirus pulmonary syndrome in south america. the purpose of this study was to determine whether rio mamoré virus strain htn-007 in syrian golden hamsters is pathogenic. none of 37 adult hamsters infected by intramuscular injection of htn-007, including 10 animals killed on day 42 or 43 post-inoculation, exhibited any symptom of disease. histological abnormalities included severe or moderately severe pneumonitis in 6 (46.2%) of the 13 animals killed ... | 2014 | 25064267 |
| experimental infection of rio mamore hantavirus in sigmodontinae rodents. | this study shows an experimental spillover infection of sigmodontinae rodents with rio mamore hantavirus (riomv). necromys lasiurus and akodon sp were infected with 103 rna copies of riomv by intraperitoneal administration. the viral genome was detected in heart, lung, and kidney tissues 18 days after infection (ai), and viral excretion in urine and faeces began at four and six ai, respectively. these results reveal that urine and faeces of infected rodents contain the virus for at least 18 days ... | 2016 | 27223653 |
| rio mamoré virus and hantavirus pulmonary syndrome, brazil. | | 2014 | 25152089 |
| maripa hantavirus in french guiana: phylogenetic position and predicted spatial distribution of rodent hosts. | a molecular screening of wild-caught rodents was conducted in french guiana, south america to identify hosts of the hantavirus maripa described in 2008 in a hantavirus pulmonary syndrome (hps) case. over a 9-year period, 418 echimyids and murids were captured. viral rna was detected in two sigmodontine rodents, oligoryzomys fulvescens and zygodontomys brevicauda, trapped close to the house of a second hps case that occurred in 2009 and an o. fulvescens close to the fourth hps case identified in ... | 2014 | 24752689 |
| [hantavirus pulmonary syndrome (rio mamore virus) in the peruvian amazon region]. | hantavirus infection is a viral zoonotic infection borne by rodents which most letal form clinical is the hantavirus pulmonary syndrome (sph, spanish abbreviation). the mamore river variant originates in south america and was found in rodents without any association to human diseases. two cases of sph were identified in the peruvian amazon region in november 2011. in both cases, a molecular diagnostic testing was conducted by the instituto nacional de salud from peru. a phylogenetic analysis of ... | 2012 | 23085803 |
| diversity and distribution of hantaviruses in south america. | hantaviruses are important contributors to disease burden in the new world, yet many aspects of their distribution and dynamics remain uncharacterized. to examine the patterns and processes that influence the diversity and geographic distribution of hantaviruses in south america, we performed genetic and phylogeographic analyses of all available south american hantavirus sequences. we sequenced multiple novel and previously described viruses (anajatuba, laguna negra-like, two genotypes of castel ... | 2012 | 23055565 |
| complete genome sequence of a novel hantavirus variant of rio mamoré virus, maripa virus, from french guiana. | we report the first complete genome sequence of maripa virus identified in 2009 from a patient with hantavirus pulmonary syndrome in french guiana. maripa virus corresponds to a new variant of the rio mamoré virus species in the bunyaviridae family, genus hantavirus. | 2012 | 22492924 |