| a survey of entamoeba histolytica and entamoeba dispar (brumpt) infections on mahé, the seychelles. | e. histolytica, pathogenic, and e. dispar, non-pathogenic, being morphologically identical are, when recovered as cysts in feces, diagnosed as e. histolytica. a survey on the seychelles demonstrated that when culture and zymodeme characterization was used to compare against microscopy alone, the risk of overdiagnosis of e. histolytica infections was drastically reduced. from a total of 313 subjects tested 21 cultures grew amebas. by zymodeme analysis eight were e. histolytica, 40 were e. dispar ... | 1992 | 1340310 |
| on the possible relationships between entamoeba moshkovskii, the laredo strains and brumpt's entamoeba dispar. | | 1969 | 5794460 |
| axenic cultivation of entamoeba dispar brumpt 1925, entamoeba insolita geiman and wichterman 1937 and entamoeba ranarum grassi 1879. | three species of entamoeba have been grown in axenic culture for the first time. in two cases, novel methods for adapting the organisms to growth without bacteria were employed. while e. ranarum was axenized by the classic technique of diamond, from a monoxenic culture with trypanosoma cruzi as the associate, both e. dispar and e. insolita were first grown in axenic culture medium supplemented with lethally irradiated bacteria. from there, e. insolita was axenized directly, but e. dispar initial ... | 1995 | 7581333 |
| [entamoeba histolytica (schaudinn 1903) and entamoeba dispar (e. brumpt 1925) are 2 different species]. | on the basis of epidemiologic and clinical data in humans, and of experimental studies in kittens, e. brumpt in 1925 showed that entamoeba histolytica producing quadrinucleate cysts was a complex of 2 species. he differentiated a pathogenic and invasive form, from a non pathogenic and non invasive one that he called entamoeba dispar. his explanation gained little support until recently, when sargeaunt reported a study of isoenzyme typing and allow then the classification of amoebae resembling e. ... | 1994 | 7866041 |
| host-parasite relationships in amebiasis: conference report. | entamoeba histolytica has been redescribed as the separate species e. histolytica and entamoeba dispar. e. dispar is apparently never invasive in humans, while e. histolytica is the etiologic agent of amebic colitis and liver abscess. virulence factors that may enable e. histolytica to invade include a galactose-specific adhesin, secreted proteases, extracellular matrix receptors, and a cell surface lipophosphoglycan. progress in vaccine development includes the identification of the cysteine-ri ... | 1994 | 7908923 |
| [microbiological diagnosis of malaria and amoebiasis]. | all stages of malaria and amebiasis may be sensitively and specifically diagnosed by means of microbiological methods. microscopical parasitology holds a key position when supplemented by immunodiagnostic assays. molecular biology techniques are currently still inferior to the above procedure. tests for the differentiation between pathogenic and nonpathogenic entamoeba histolytica (entamoeba dispar) require further simplification before generally applicable. | 1994 | 7959857 |
| cloning of entamoeba genes encoding proteolipids of putative vacuolar proton-translocating atpases. | molecular cloning techniques were used to identify genes encoding the proteolipids of putative vacuolar proton-transporting atpases (v-atpases; ec 3.6.1.35) of entamoeba histolytica (ehvma3) and entamoeba dispar. the ehvma3 gene encoded a 177-amino-acid peptide, with an m(r) of 18,110, which showed extensive positional identities with peptides of e. dispar (92%), schizosaccharomyces pombe (58%), and humans (56%). | 1994 | 8039932 |
| entamoeba histolytica: an explanation for the reported conversion of "nonpathogenic" amebae to the "pathogenic" form. | the reported conversion of "nonpathogenic" entamoeba histolytica isolates to the "pathogenic" form during attempted axenization of the amebae is highly controversial. after failing to obtain conversions ourselves we concluded that the simplest explanation for the published observations would be contamination of nonpathogenic cultures with pathogenic amebae. to address this possibility we used a method based on analysis of stable dna polymorphisms that allows the positive identification of indivi ... | 1993 | 8253159 |
| a redescription of entamoeba histolytica schaudinn, 1903 (emended walker, 1911) separating it from entamoeba dispar brumpt, 1925. | explaining the low incidence of invasive disease (10%) in humans infected with entamoeba histolytica has occupied the attention of generations of both clinical and nonclinical investigators. one possible explanation would be the existence of two morphologically identical species-one an invasive pathogen, the other noninvasive. this was first proposed by brumpt in 1925, but his explanation was virtually ignored until 1978 when the first of several publications appeared suggesting that e. histolyt ... | 1993 | 8508172 |
| rapid diagnosis of entamoeba infection by using entamoeba and entamoeba histolytica stool antigen detection kits. | humans are infected by two morphologically identical species of entamoeba: entamoeba histolytica causes amebic colitis and liver abscess, and entamoeba dispar is noninvasive. several weeks of culture and isoenzyme (zymodeme) analysis are required to differentiate e. histolytica from e. dispar. here we report a field trial of commercial antigen detection kits designed to rapidly detect and differentiate e. histolytica from e. dispar in stool specimens. stool specimens from 202 patients with diarr ... | 1995 | 8567882 |
| a homologue of the cysteine proteinase gene (acp1 or eh-cpp3) of pathogenic entamoeba histolytica is present in non-pathogenic e. dispar strains. | one of the three cysteine proteinase genes, acp1 (or cp 3), has been reported to be missing in non-pathogenic strains of entamoeba histolytica (or entamoeba dispar as recently labeled). unexpectedly, a gene fragment very similar in its sequence (95% homology) to acp1 of pathogenic strains was obtained by use of the polymerase chain reaction from genomic dna and cdna of various cloned non-pathogenic strains as well as in 23 clinical isolates from asymptomatic carriers. the finding of the acp1 hom ... | 1996 | 8813676 |
| differentiation of korean isolates of entamoeba histolytica from entamoeba dispar. | cysts of entamoeba histolytica are still found from humans in korea, but not all of the cysts are known as pathogenic. the non-pathogenic strain is regarded as a different species, e. dispar. in this study, korean isolates of conventional e. histolytica were subjected for the differentiation by polymerase chain reaction (pcr) and restriction fragment length polymorphism (rflp) analysis. human stools were screened by routine microscopic examination, and cyst or trophozoite positive stools were in ... | 1996 | 8820737 |
| new concepts regarding the pathogenesis of amebiasis. | our understanding of the pathogenesis of amebiasis has progressed significantly since dr. braude's description of the major clinical syndromes of amebic liver abscess. his hypothesis that invasive amebic strains must be resistant to complement-mediated lysis has been confirmed. we have also shown that entamoeba histolytica activates complement by a unique mechanism, i.e., cleavage of c3 by an extracellular cysteine proteinase. cysteine proteinases are important virulence factors encoded by at le ... | 1995 | 8845450 |
| entamoeba histolytica and entamoeba dispar: differences in numbers and expression of cysteine proteinase genes. | in order to identify molecules that might be responsible for the difference in pathogenicity between the two closely related protozoan parasites entamoeba histolytica and entamoeba dispar, we focussed on cysteine proteinases because this class of enzymes has been considered important for pathogenic tissue destruction. by screening a genomic library derived from an e. histolytica isolate, a total of six distinct genes (ehcp1-ehcp6) encoding typical prepro-forms of cysteine proteinases were identi ... | 1996 | 8930910 |
| direct sequencing of the pcr amplified ssu rrna gene of entamoeba dispar and the design of primers for rapid differentiation from entamoeba histolytica. | since 1993, strains of entamoeba histolytica sensu lato have been assigned to 2 species on the basis of clinical, biochemical, immunological and genetic evidence: the pathogenic strains to e. histolytica sensu stricto, the non-pathogenic strains to entamoeba dispar. analysis of the gene encoding for the small subunit ribosomal rna (ssu rdna) supports the existence of 2 species. however, while 3 whole ssu rdna sequences are available in the data bases for e. histolytica, only a partial sequence h ... | 1996 | 8935948 |
| differentiation of entamoeba histolytica and entamoeba dispar in cyst-passers by immunoblot. | differentiation of invasive strains of entamoeba histolytica according to their pathogenicity has been a topic of long debate, but now the pathogenic species only is regarded as e. histolytica while the non pathogenic species is e. dispar. the present study applied immunoblot to differentiate infections of the two species among microscopically-detected cyst-passers in korea. the crude extract of e. histolytica separated in 5.20% gradient gels, revealed many fractions of 94, 81, 71, 50, 44, 38.5, ... | 1996 | 9017910 |
| stable episomal transfection and gene expression in entamoeba dispar. | | 1996 | 9027760 |
| molecular cloning of histone h1 in entamoeba histolytica and histone h2b in entamoeba histolytica and entamoeba dispar. | | 1997 | 9032995 |
| transfection of entamoeba dispar: inhibition of expression of the lysine-rich 30 kda surface antigen by the transcription of its antisense rna. | | 1997 | 9033010 |
| molecular cloning and biochemical characterization of hexokinases and phosphoglucomutases from entamoeba histolytica and entamoeba dispar. | | 1997 | 9033021 |
| entamoeba dispar: cultivation without viable associate and its characterization. | | 1997 | 9033032 |
| comparative ultrastructural studies of the cell surface and endocytic vacuoles of entamoeba histolytica schaudinn, 1903 and entamoeba dispar brumpt, 1925. | | 1997 | 9033034 |
| entamoeba dispar: ultrastructure and cytopathic effect. | | 1997 | 9033035 |
| development of monoclonal antibodies directed against cysts of entamoeba histolytica and entamoeba dispar. | | 1997 | 9033048 |
| structure and function of the galactose/n-acetyl d-galactosamine inhibitable adhesin of entamoeba dispar. | | 1997 | 9033057 |
| in vivo pathogenesis of entamoeba dispar. | | 1997 | 9033073 |
| a proposal for a molecular biologic system for classifying isolates of entamoeba histolytica and entamoeba dispar. | | 1997 | 9033099 |
| diagnosis of entamoeba histolytica and entamoeba dispar in clinical specimens by pcr-shela. | | 1997 | 9033102 |
| entamoeba histolytica and entamoeba dispar infection in children in bangladesh. | | 1997 | 9033116 |
| entamoeba histolytica and entamoeba dispar infection in children in bangladesh. | the prevalence of infection by the invasive parasite entamoeba histolytica and the noninvasive parasite entamoeba dispar was determined in 2000 children in bangladesh. antigen detection identified more cases of e. histolytica-e. dispar infection than did culture or microscopy. microscopic identification of e. histolytica-e. dispar complex infection in stool did not equate with the diagnosis of amebic dysentery because most amebic infections in this population were due to e. dispar: urban childre ... | 1997 | 9041357 |
| cloning and expression of chitinases of entamoebae. | entamoeba histolytica (eh) and entamoeba dispar (ed) are protozoan parasites that infect hundreds of millions of persons. in the colonic lumen, amebae form chitin-walled cysts, the infectious stage of the parasite. entamoeba invadens (ei), which infects reptiles and is a model for amebic encystation, produces chitin synthase and chitinase during encystation. ei cysts formation is blocked by the chitinase-inhibitor allosamidin. here molecular cloning techniques were used to identify homologous ge ... | 1997 | 9106188 |
| an improved colorimetric pcr-based method for detection and differentiation of entamoeba histolytica and entamoeba dispar in feces. | the epidemiological implications of the recent separation of "entamoeba histolytica" into two separate species, pathogenic e. histolytica sensu stricto and commensal e. dispar, will not become apparent without methods of distinguishing between them which are applicable to large numbers of specimens. we have modified a pcr-based method to produce such a technique which may be completed in 1 day while still identifying 10(-1) e. histolytica and 1 to 10 e. dispar trophozoites per g of feces when pr ... | 1997 | 9114390 |
| molecular biology of the hexokinase isoenzyme pattern that distinguishes pathogenic entamoeba histolytica from nonpathogenic entamoeba dispar. | the electrophoretic patterns of hexokinase and phosphoglucomutase have been widely used to distinguish entamoeba histolytica from entamoeba dispar isolates. although e. histolytica and e. dispar, previously called pathogenic and nonpathogenic entamoeba histolytica, differ clearly in sequences of many homologous genes, a conversion between the two has been reported by several laboratories, in each case showing the conversion of hexokinase (atp, d-hexose 6-phosphotransferase, ec 2.7.1.1) isoenzyme ... | 1997 | 9178270 |
| simple differential detection of entamoeba histolytica and entamoeba dispar in fresh stool specimens by sodium acetate-acetic acid-formalin concentration and pcr. | amoebiasis is caused by two distinct species, a pathogenic form (entamoeba histolytica) and a nonpathogenic form (entamoeba dispar), which are morphologically identical. although the distinction between these two species is of great clinical importance, the methods developed for this purpose either are very time-consuming or involve laborious procedures for isolation of the dna. we report here a simple pcr method starting with fresh stool specimen that allows for the sensitive and reliable disti ... | 1997 | 9196177 |
| preparation of a monoclonal antibody specific for entamoeba dispar and its ability to distinguish e. dispar from e. histolytica. | a monoclonal antibody (mab), mab ed17 (immunoglobulin g2a [igg2a]), prepared against trophozoites of entamoeba dispar saw1734rclar cultured monoxenically with crithidia fasciculata, reacted with 25 of 26 isolates of e. dispar by an indirect fluorescent-antibody test. in contrast, the mab failed to react with any of 20 isolates of e. histolytica or other enteric protozoan parasites. western blot (immunoblot) analysis showed that the molecular mass of the e. dispar antigen recognized by the mab wa ... | 1997 | 9220156 |
| invasive amoebiasis in two patients with aids and cytomegalovirus colitis. | homosexual persons or human immunodeficiency virus (hiv)-infected patients frequently excrete cysts of nonpathogenic strains of entamoeba histolytica ("entamoeba dispar"). however, invasive amoebiasis is rare. we report two patients with aids and cytomegalovirus colitis in whom invasive amoebiasis was histologically diagnosed. it is concluded that e. histolytica has to be considered in hiv-infected patients with colitis. | 1997 | 9230408 |
| a gene homologous to hgl2 of entamoeba histolytica is present and expressed in entamoeba dispar. | | 1997 | 9233678 |
| differentiation of entamoeba histolytica and entamoeba dispar from german travelers and residents of endemic areas. | entamoeba histolytica and e. dispar detected in fecal samples of german travelers and residents of tropical and subtropical countries were cultured and differentiated by the polymerase chain reaction (pcr) and hexokinase isoenzyme typing. twenty-one of 60 isolates were identified as e. histolytica. it was shown that 13 of 18 german short-term travelers were infected with e. histolytica, whereas only three of 22 of the examined inhabitants of tropical countries and five of 20 german long-term tra ... | 1997 | 9242322 |
| protection against invasive amebiasis by a single monoclonal antibody directed against a lipophosphoglycan antigen localized on the surface of entamoeba histolytica. | a panel of monoclonal antibodies was raised from mice immunized with a membrane preparation from entamoeba histolytica, the pathogenic species causing invasive amebiasis in humans. antibody eh5 gave a polydisperse band in immunoblots from membrane preparations from different e. histolytica strains, and a much weaker signal from two strains of the nonpathogenic species entamoeba dispar. although the exact chemical structure of the eh5 antigen is not yet known, the ability of the antigen to be met ... | 1997 | 9348313 |
| does entamoeba histolytica cause irritable bowel syndrome? | symptoms of patients with irritable bowel syndrome (ibs) closely mimic those of patients with non-dysenteric amebic colitis. | 1997 | 9357182 |
| intestinal parasitic infections in patients with malignancy. | the frequency of intestinal parasitic infections was studied retrospectively in 1,029 cancer patients presenting with symptoms of diarrhoea. intestinal parasites were diagnosed by stool examination, using both the direct and concentration techniques and also the modified acid fast stain. parasitic infection was found in 16.5% of the cases. the majority of the patients with intestinal parasitosis had cancer of the haemopoietic system and were on anticancer chemotherapy. the most prevalent parasit ... | 1997 | 9360344 |
| sensitivity of entamoeba histolytica and entamoeba dispar patient isolates to human complement. | twenty-one entamoeba histolytica and 56 entamoeba dispar patient isolates were investigated for their sensitivity to the classical and alternative pathway of human complement, e. histolytica and e. dispar patient isolates were differentiated by polymerase chain reaction and hexokinase isoenzyme typing. it was found that 90.3% (+/- 12.0%) of the trophozoites of e. histolytica were lysed after 30 min by the alternative pathway of complement in the presence of 50% human serum (19 isolates showed ly ... | 1997 | 9364556 |
| prospective study of entamoeba dispar infection in a cohort of mothers and their infants: relationship to serum antibody response. | the sera of cohorts of newborn infants and their mothers, characterized as cyst passers of entamoeba with nonpathogenic zymodemes (e. dispar) and seropositive for amoebic antigens, were analyzed. both cohorts were followed for a period of 12 months by microscopic examination of feces and determination of serum anti-amoebic antibody titers using the indirect hemagglutination assay. control groups (noncyst passer mothers and their infants) were included and followed. to characterize antigens invol ... | 1997 | 9392591 |
| isolation and molecular characterization of a surface-bound proteinase of entamoeba histolytica. | major pathogenic functions of entamoeba histolytica involved in destruction of host tissues are the degradation of extracellular matrix proteins mediated by secreted cysteine proteinases and contact-dependent killing of host cells via membrane-active factors. a soluble protein with an affinity for membranes was purified from amoebic extracts to apparent homogeneity. n-terminal sequencing and subsequent molecular cloning of the factor revealed that it is a member of the cysteine proteinase family ... | 1998 | 9484883 |
| molecular and biochemical characterization of phosphoglucomutases from entamoeba histolytica and entamoeba dispar. | entamoeba histolytica and entamoeba dispar have only recently been defined as two separate species. e. histolytica, the pathogenic species, is the microorganism causing invasive intestinal amoebiasis and/or liver abscess, while the morphologically similar e. dispar is nonpathogenic and noninvasive. the gold standard for the distinction of the two species has been the isoenzyme electrophoresis of phosphoglucomutases (ec 5.4.2.2) and hexokinases (ec 2.7.1.1), but there had also been a controversy ... | 1997 | 9497037 |
| inhibition of expression of the lysine-rich 30 kda surface antigen of entamoeba dispar by the transcription of its antisense rna. | the gene coding for the 30 kda lysine rich surface antigen (ed-ag) that is present on membrane surfaces of entamoeba dispar trophozoites has been characterized. a specific monoclonal antibody mab 318-28 prepared against this antigen reacts with all e. dispar strains tested, but not with any of the antigens of e. histolytica. in order to understand the function of this antigen, we constructed two plasmids, peda-9 and peda-rev, in which the antigen-coding sequence was introduced into the pehact-ne ... | 1997 | 9497043 |
| entamoeba histolytica and entamoeba dispar are distinct species; clinical, epidemiological and serological evidence. | the name of the causative organism of invasive amoebiasis, entamoeba histolytica, was first introduced in 1903, even though this intestinal amoeba had been recognised since 1875. the marked disparity between the number of infected individuals and those with invasive amoebiasis resulted in a number of explanatory hypotheses being proposed. although none of these were universally accepted, brumpt's concept of two morphologically identical species gained increasing acceptance 50-60 years later when ... | 1998 | 9504344 |
| entamoeba dispar: cultivation with sterilized crithidia fasciculata. | four isolates of entamoeba dispar identified by their hexokinase and phosphoglucomutase isoenzyme profile and by their failure to react with entamoeba histolytica-specific monoclonal antibody (4g6) could be grown in either diamond's bi-s-33 medium, newly developed bcsi-s (biosate cysteine starch iron-serum) medium, or casein-free yi-s medium in the presence of crithidia fasciculata (ref-1:prr) sterilized by heating 56 degrees c for 30 min and subsequent incubation with 1% hydrogen peroxide for 2 ... | 1998 | 9561776 |
| the bactericidal activity of blood platelets in patients infested with entamoeba histolytica/entamoeba dispar. | blood platelets participate actively in organism defensive reactions and particularity in antiparasitic immunity. platelets stimulation can be the result of contact with a parasite, the presence of immunoglobulins, complement and lymphokines. the aim of the study was to evaluate the blood platelets bactericidal activity in the course of amoebiasis. the examination was carried out in the group of 22 patients with e. histolytica/e. dispar, diagnosed on the basis of cyst forms presence in faeces. t ... | 1997 | 9581473 |
| evaluation of intestinal protozoan morphology in human fecal specimens preserved in ecofix: comparison of wheatley's trichrome stain and ecostain. | as a result of disposal problems related to the use of mercury compounds, many laboratories have switched from mercuric chloride-based schaudinn's and polyvinyl alcohol (pva) stool preservatives to other, non-mercury-based preservatives. a comparison of organism recoveries and morphologies of the intestinal protozoa was undertaken with pva containing the ecofix zinc-based schaudinn's preservative (meridian diagnostics, inc.); both wheatley's modification of gomori's trichrome stain (wt) and ecos ... | 1998 | 9650947 |
| entamoeba dispar: ultrastructure, surface properties and cytopathic effect. | the cytological features of entamoeba dispar, recently recognized by biochemical and molecular biology criteria as a distinct species, were compared to those of entamoeba histolytica when cultured under axenic conditions, living trophozoites of e. dispar strain saw 76orr clone a were more elongated in form, had a single frontal pseudopodium, and showed a noticeable uroid. in sections of e. dispar trophozoites stained with toluidine blue, characteristic areas of cytoplasmic metachromasia were see ... | 1998 | 9669863 |
| entamoeba histolytica and entamoeba dispar in the northeast brazil. | | 1997 | 9674291 |
| seropositivity for and intestinal colonization with entamoeba histolytica and entamoeba dispar in individuals in northeastern brazil. | in a slum community in northeastern brazil 20% of a sample population was colonized with entamoeba histolytica or entamoeba dispar and 10.6% was colonized with e. histolytica alone. no correlation between seropositivity for anti-ga1nac lectin antibody and colonization was found. these results suggest that colonization does not necessarily produce immunity to reinfection. | 1998 | 9738064 |
| royal society of tropical medicine and hygiene meeting at manson house, london, 19 february 1998. amoebic disease. entamoeba dispar, an organism reborn. | entamoeba dispar was described by emile brumpt in 1925, and was promptly dismissed as a synonym of e. histolytica by almost all his contemporaries. in the 1970s, however, evidence began to accumulate indicating that brumpt may have been correct and e. dispar is now accepted as a distinct species. this review introduces the organism's history and briefly summarizes what we currently know about its biology. the acceptance of e. dispar has profound implications for our understanding of the epidemio ... | 1998 | 9850382 |
| field study on the distribution of entamoeba histolytica and entamoeba dispar in the northern philippines as detected by the polymerase chain reaction. | we used the polymerase chain reaction (pcr) to study the distribution of entamoeba histolytica and e. dispar in 1,872 individuals in 14 communities in the northern philippines. here we report a field study using a dna extraction protocol from formalin-fixed stool specimens as previously reported. this assay detected 137 stools (7.318%) containing e. dispar and 18 stools (0.961%) containing e. histolytica. the most affected age group for e. histolytica/e. dispar infections were those 5-14 years o ... | 1998 | 9886200 |
| isoenzyme and molecular characterization of entamoeba histolytica and entamoeba dispar isolates from symptomatic and asymptomatic subjects. | to correlate the clinical features of amebic infections with the characteristics of entamoeba culture isolates of stools. | 1999 | 10063741 |
| [pleuropulmonary manifestations of amebiasis]. | amibiasis is the third leading cause of death due to parasitic infections in the world. amibiasis is endemic in the warm regions of the world with deficient hygiene and socio-economic situations. entamoeba histolytica is the causal agent of invasive amibiasis, unlike entamoeba dispar which is not a pathogen for humans. amibian colitis and amibian abscess of the liver are the most frequent intestinal and extra-intestinal manifestations. pleuropulmonary complications almost always occur in patient ... | 1998 | 10100347 |
| preparation of recombinant human monoclonal antibody fab fragments specific for entamoeba histolytica. | genes coding for human antibody fab fragments specific for entamoeba histolytica were cloned and expressed in escherichia coli. lymphocytes were separated from the peripheral blood of a patient with an amebic liver abscess. poly(a)+ rna was isolated from the lymphocytes, and then genes coding for the light chain and fd region of the heavy chain were amplified by a reverse transcriptase pcr. the amplified dna fragments were ligated with a plasmid vector and were introduced into escherichia coli. ... | 1999 | 10225840 |
| identification of novel genes of non-pathogenic entamoeba dispar by expressed sequence tag analysis. | | 1999 | 10340493 |
| detection of entamoeba dispar dna in macaque feces by polymerase chain reaction. | we used the polymerase chain reaction (pcr) to determine the prevalence of entamoeba histolytica and e. dispar in the wild population of macaque monkeys (macaca fuscata) in mt. takasaki, oita prefecture, japan. of the 101 samples collected, 41 (42.57%) were found to be positive for e. dispar. however, no e. histolytica was detected from the collected samples. the results of this survey demonstrate the high prevalence of e. dispar in macaque monkeys in the study area. moreover, they provide addit ... | 1999 | 10344544 |
| prevalence and immune response to entamoeba histolytica infection in preschool children in bangladesh. | entamoeba histolytica infection was present in 5% and e. dispar in 13% of asymptomatic 2-5-year-old children from an urban slum of dhaka, bangladesh. entamoeba dispar-infected children were no more likely than uninfected children to have serum antibodies to lectin. in contrast, all children infected with e. histolytica had serum antibodies to lectin. this anti-lectin response included antibodies against the carbohydrate recognition domain, which have been demonstrated in animal models to confer ... | 1999 | 10403338 |
| entamoeba dispar contains but does not secrete acid phosphatase as does entamoeba histolytica. | | 1999 | 10403764 |
| pore-forming peptides of entamoeba dispar. similarity and divergence to amoebapores in structure, expression and activity. | amoebapore, a 77-residue peptide with pore-forming activity from the human pathogen entamoeba histolytica, is implicated in the killing of phagocytosed bacteria and in the cytolytic reaction of the amoeba against host cells. previously, we structurally and functionally characterized three amoebapore isoforms in e. histolytica but recognized only one homolog in the closely related but non-pathogenic species entamoeba dispar. here, we identified two novel amoebapore homologs from e. dispar by mole ... | 1999 | 10518795 |
| entamoeba histolytica and entamoeba dispar: epidemiology and comparison of diagnostic methods in a setting of nonendemicity. | recent studies suggest that stool antigen assays are more sensitive and specific than microscopy for the diagnosis of entamoeba histolytica infection. one hundred twelve patients presenting at 3 centers with symptoms or risk factors of e. histolytica infection were prospectively enrolled in this study to evaluate new diagnostic tests for infections with e. histolytica and entamoeba dispar. four elisa-based stool antigen kits for detecting e. histolytica or e. dispar were blindly compared with st ... | 1999 | 10524983 |
| a dna sequence corresponding to the gene encoding cysteine proteinase 5 in entamoeba histolytica is present and positionally conserved but highly degenerated in entamoeba dispar. | cysteine proteinases of entamoeba histolytica are considered to be one of the most important classes of molecules responsible for the parasite's ability to destroy human tissues. interestingly, one particular cysteine proteinase, located on the surface of e. histolytica trophozoites and designated cysteine proteinase 5 (cp5), is not expressed in the closely related but nonpathogenic species entamoeba dispar. by comparing the e. histolytica and e. dispar genomic loci containing the gene for cp5 ( ... | 1999 | 10531249 |
| characterization of the gene encoding serine acetyltransferase, a regulated enzyme of cysteine biosynthesis from the protist parasites entamoeba histolytica and entamoeba dispar. regulation and possible function of the cysteine biosynthetic pathway in entamoeba. | the enteric protist parasites entamoeba histolytica and entamoeba dispar possess a cysteine biosynthetic pathway, unlike their mammalian host, and are capable of de novo production of l-cysteine. we cloned and characterized cdnas that encode the regulated enzyme serine acetyltransferase (sat) in this pathway from these amoebae by genetic complementation of a cysteine-auxotrophic escherichia coli strain with the amoebic cdna libraries. the deduced amino acid sequences of the amoebic sats exhibite ... | 1999 | 10542289 |
| an improved method to distinguish entamoeba histolytica and entamoeba dispar. | a 482 base pair gene fragment from samples of amoebae e. histolytica and e. dispar was amplified by pcr. the amplification products of fragments from the 2 species of amoebae presented differences in mobility in non-denaturing polyacrylamide gel, probably due to sequence-dependent conformational alterations in the dna fragments. the method described here permits e. histolytica and e. dispar to be distinguished with greater sensitivity and rapidity. | 1999 | 10581613 |
| differences in substrate specificity and kinetic properties of the recombinant hexokinases hxk1 and hxk2 from entamoeba histolytica. | entamoeba histolytica is responsible for amoebic colitis and liver abscess in humans. entamoeba dispar is a closely related, morphologically indistinguishable nonpathogenic species. the hexokinase (atp:d-hexose 6-phosphotransferase, ec 2.7.1.1) isoenzyme patterns distinguish the pathogenic and nonpathogenic species. both species possess two hexokinases with very similar molecular mass and different isoelectric points. in order to understand the role of the two different isoenzymes from e. histol ... | 2000 | 10613700 |
| application of the polymerase chain reaction (pcr) in the epidemiology of entamoeba histolytica and entamoeba dispar infections. | in this paper, we briefly summarize the latest information on the polymerase chain reaction (pcr) as an epidemiologic tool for entamoeba histolytica and entamoeba dispar infections. this method which employs dna template directly extracted from formalin fixed stool specimens offers a good promise for an accurate and reliable epidemiology of the two species. the assay is, sensitive enough to detect as few as five cysts in the stool sample, rapid and selectively differentiates e. histolytica from ... | 1998 | 10622639 |
| entamoeba dispar: cloning and characterization of peroxiredoxin genes. | | 2000 | 10631082 |
| intestinal protozoa in homosexual men in edinburgh. | our objective was to determine the prevalence of intestinal protozoa in homosexual men attending a sexually transmitted diseases (std) clinic, to compare it with the prevalence in a previous study from the same clinic, and to examine the relationship, if any, between the presence of protozoa and sexual practices. men who consecutively attended the clinic and who gave a history of having had recent sexual contact with another male were invited to provide a stool sample for protozoological examina ... | 1999 | 10639057 |
| importance of differentiation of entamoeba histolytica from entamoeba dispar. | | 1999 | 10647224 |
| inhibition of gene expression in entamoeba by the transcription of antisense rna: effect of 5' and 3' regulatory elements. | down regulation of gene expression by antisense rna is one of the ways to investigate the specific contribution of certain components to the physiology and activities of a cell. a successful inhibition of gene expression in entamoeba trophozoites was achieved in stable transfectants by using hybrid plasmid constructs containing promotors that produce transcripts which do not bind to polysomes. different promotors were found to be required for entamoeba histolytica or entamoeba dispar. in e. hist ... | 2000 | 10717304 |
| cloning and biochemical characterization of genes encoding two isozymes of cysteine synthase from entamoeba dispar. | | 2000 | 10717309 |
| absence of lipophosphoglycan-like glycoconjugates in entamoeba dispar. | invasive amoebiasis is the result of infection of entamoeba histolytica. the closely related entamoeba dispar can colonize the human gut but does not cause invasive disease. in this study, e. dispar was analysed for the presence of the lipophosphoglycan-like (lpg) glycoconjugate known to be present on the cell surface of e. histolytica. e. dispar cells were radio-isotope labelled with [3h]galactose or [3h]inositol. the acidic glycoconjugates were extracted and analysed by hydrophobic chromatogra ... | 2000 | 10726263 |
| asymptomatic cyst passers of entamoeba histolytica but not entamoeba dispar in institutions for the mentally retarded in japan. | entamoeba histolytica/entamoeba dispar was isolated from 50 asymptomatic amebic cyst passers in three institutions for the mentally retarded in kanagawa prefecture, japan. to distinguish between e. histolytica and e. dispar, the isolates were analyzed by pcr, reactivity to monoclonal antibodies, and zymodemes. all isolates were identified as e. histolytica. the results lead us to conceive that, in japan, e. histolytica is predominant even in asymptomatic cyst passers. | 2000 | 10729715 |
| comparison of polyvinyl alcohol fixative with three less hazardous fixatives for detection and identification of intestinal parasites. | polyvinyl alcohol (pva) containing the fixative mercuric chloride is considered the "gold standard" for the fixation of ova and parasites in the preparation of permanently stained smears of stool specimens. however, mercuric chloride is potentially hazardous to laboratory personnel and presents disposal problems. we compared three new alternative, nontoxic fixatives with pva, analyzing ease of sample preparation and quality of smears. sixty-eight fresh stool specimens were divided into aliquots ... | 2000 | 10747149 |
| enteropathogens in adult patients with diarrhea and healthy control subjects: a 1-year prospective study in a swedish clinic for infectious diseases. | a 1-year prospective study was conducted to identify enteropathogens in adults with diarrhea (n=851) and in healthy control subjects (n=203) by use of conventional laboratory methods. virulence factor genes for diarrheagenic escherichia coli were detected by polymerase chain reaction. enteropathogens were identified in 56% of patients and 16% of control subjects. the isolation rate was 65% for patients with symptoms for <1 week and for travelers; >1 pathogen was found in 11% of patients. the mos ... | 2000 | 10816147 |
| diagnosis of invasive amebiasis by enzyme-linked immunosorbent assay of saliva to detect amebic lectin antigen and anti-lectin immunoglobulin g antibodies. | saliva from subjects with amebic liver abscess (ala), acute amebic colitis, asymptomatic infection with entamoeba histolytica or entamoeba dispar, and uninfected controls was tested by enzyme-linked immunosorbent assay (elisa) for the presence of e. histolytica galactose-inhibitable lectin antigen and salivary immunoglobulin (igg) antibodies to a recombinant cysteine-rich lectin-derived protein (lc3). salivary lectin antigen was found in 65.8% of subjects with acute colitis, compared to 22.2% of ... | 2000 | 10835000 |
| inter- and intra-strain variation in the 5.8s ribosomal rna and internal transcribed spacer sequences of entamoeba histolytica and comparison with entamoeba dispar, entamoeba moshkovskii and entamoeba invadens. | the ribosomal rna genes in entamoeba histolytica are located on circular dna molecules in about 200 copies per genome equivalent. nucleotide sequence analysis of the 5.8s rrna gene and the flanking internal transcribed spacers was carried out to determine the degree of sequence divergence in the multiple rrna gene copies of a given strain; amongst three different e. histolytica strains (hm-1:imss, rahman and hk-9); and amongst four species of entamoeba (entamoeba histolytica, entamoeba dispar, e ... | 2000 | 10856506 |
| entamoeba histolytica/entamoeba dispar infections in human immunodeficiency virus-infected patients in the united states. | we describe the incidence of and laboratory and clinical characteristics associated with entamoeba histolytica/entamoeba dispar infection diagnosed in human immunodeficiency virus (hiv)-infected persons enrolled in the adult and adolescent spectrum of hiv disease project. from 1 january 1990 to 1 january 1998 (82, 518 person-years of follow-up), 111 patients (98% men) were diagnosed with e. histolytica/e. dispar infection. among hiv-infected patients in the united states, the incidence of diagno ... | 2000 | 10880314 |
| a nested, multiplex, pcr assay for the simultaneous detection and differentiation of entamoeba histolytica and entamoeba dispar in faeces. | the detection of and differentiation between entamoeba histolytica and entamoeba dispar are of great importance, both for diagnosis and for epidemiological studies. most pcr-based methods for the discrimination of these two species employ complex procedures for dna extraction and require different protocols for e. histolytica and e. dispar, leading to relatively high expenditure, labour costs and turnaround times. a simple, rapid, cost-effective and yet sensitive and specific multiplex pcr techn ... | 2000 | 10884867 |
| differentiation of entamoeba histolytica and entamoeba dispar cysts using polymerase chain reaction on dna isolated from faeces with spin columns. | since entamoeba histolytica and entamoeba dispar were formally recognized as two different species at the world health organization (who)/pan american health organization (paho)/united nations educational, scientific and cultural organization (unesco) meeting in mexico city in 1997, the specific differentiation of the two morphologically identical species would seem relevant in clinical diagnosis. several polymerase chain reaction (pcr)-based methods have been described and used successfully, bu ... | 2000 | 10898137 |
| entamoeba dispar, but not e. histolytica, detected in a colony of chimpanzees in japan. | chimpanzees (pan troglodytes) residing in the kumamoto primate research park, sanwa kagaku kenkyusho, were surveyed for the presence of intestinal parasites. stool samples from 107 chimpanzees were examined by microscopy after formalin-ether sedimentation. of these animals, 100 were infected with at least 1 species of ameba. the positivity rates recorded were as follows: entamoeba coli, 88%; e. histolytica/e. dispar, 48%; e. hartmanni, 15%; iodamoeba buetschlii, 8%; endolimax nana, 4%; and entam ... | 2000 | 10935902 |
| diagnosis of amebic liver abscess and intestinal infection with the techlab entamoeba histolytica ii antigen detection and antibody tests. | a noninvasive diagnostic test for amebic liver abscess is needed, because amebic and bacterial abscesses appear identical on ultrasound or computer tomography and because it is rarely possible to identify entamoeba histolytica in stool specimens from patients with amebic liver abscess. here we report a method of detection in serum of circulating e. histolytica gal/galnac lectin to diagnose amebic liver abscess, which was used in patients from dhaka, bangladesh. the techlab e. histolytica ii test ... | 2000 | 10970364 |
| detection of giardia lamblia, entamoeba histolytica/entamoeba dispar, and cryptosporidium parvum antigens in human fecal specimens using the triage parasite panel enzyme immunoassay. | the triage parasite panel (biosite diagnostics, san diego, calif.) is a new qualitative enzyme immunoassay (eia) panel for the detection of giardia lamblia, entamoeba histolytica/e. dispar, and cryptosporidium parvum in fresh or fresh, frozen, unfixed human fecal specimens. by using specific antibodies, antigens specific for these organisms are captured and immobilized on a membrane. panel performance was evaluated with known positive and negative stool specimens (a total of 444 specimens) that ... | 2000 | 10970380 |
| molecular epidemiology of entamoeba spp.: evidence of a bottleneck (demographic sweep) and transcontinental spread of diploid parasites. | entamoeba histolytica causes amebic colitis and liver abscess in developing countries such as mexico and india. entamoeba dispar is morphologically identical but is not associated with disease. here we determined the ploidy of e. histolytica and developed pcr-based methods for distinguishing field isolates of e. histolytica or e. dispar. fluorescence in situ hybridization showed that e. histolytica trophozoites are diploid for five "single-copy" probes tested. intergenic sequences between supero ... | 2000 | 11015408 |
| a simple stool concentration method for the detection and preservation of the vegetative forms of entamoeba histolytica/entamoeba dispar. | | 2000 | 11070212 |
| cytological and immunological methods to identify entamoeba histolytica and entamoeba dispar trophozoites in patients from guanajuato, mexico. | | 2000 | 11070213 |
| prevalence of entamoeba histolytica and entamoeba dispar in a highly endemic rural population. | | 2000 | 11070214 |
| differentiation of entamoeba histolytica and entamoeba dispar using pcr-shela and comparison of antibody response. | | 2000 | 11070218 |
| incidence of entamoeba histolytica/entamoeba dispar in international travelers, extracommunitary immigrants, and adopted children. | | 2000 | 11070219 |
| determination of entamoeba histolytica and entamoeba dispar infection by enzyme-linked immunosorbent assay (elisa) and its clinical correlation in pediatric patients. | | 2000 | 11070222 |
| does entamoeba dispar produce monocyte locomotion inhibitory factor (mlif) like entamoeba histolytica? | | 2000 | 11070239 |
| ultrastructural evidence of smooth endoplasmic reticulum and golgi-like elements in entamoeba histolytica and entamoeba dispar. | | 2000 | 11070268 |
| cultivation of entamoeba dispar: growth-promoting effect of ferredoxin. | | 2000 | 11070287 |
| entamoeba dispar galactose/n-acetyl-d-galactosamine lectin: evidence for differential gene expression and conformational regulation. | | 2000 | 11070297 |
| comparison of xenic and monoxenic entamoeba dispar cultures using hepatic inoculation in hamster. | | 2000 | 11070302 |
| characterization of entamoeba histolytica and entamoeba dispar by biological, biochemical, and molecular parameters. | | 2000 | 11070303 |
| genetic differences between entamoeba histolytica and entamoeba dispar. | | 2000 | 11070305 |