| growth of a wild strain and of a pimelic acid-utilizing mutant of pseudomonas azelaica on aliphatic dicarboxylic acids. | | 1974 | 4436649 |
| purification and characterization of 2-hydroxybiphenyl 3-monooxygenase, a novel nadh-dependent, fad-containing aromatic hydroxylase from pseudomonas azelaica hbp1. | 2-hydroxybiphenyl 3-monooxygenase (hbpa), the first enzyme of 2-hydroxybiphenyl degradation in pseudomonas azelaica hbp1, was purified 26-fold with a yield of 8% from strain hbp1 grown on 2-hydroxybiphenyl. the enzyme was also purified from a recombinant of escherichia coli jm109, which efficiently expressed the hbpa gene. computer densitometry of scanned slab gels revealed a purity of over 99% for both enzyme preparations. gel filtration, subunit cross-linking, and sds-polyacrylamide gel electr ... | 1997 | 9305879 |
| an acidic glutaryl-7-aminocephalosporanic acid acylase from pseudomonas nitroreducens. | a glutaryl-7-aminocephalosporanic acid (gl-7-aca) acylase was purified 58-fold from pseudomonas nitroreducens in a two-step procedure involving osmotic shock and carboxymethyl-sepharose chromatography with a yield of 26%. the molecular mass of the native enzyme was 58 kda. sds/page revealed that it consisted of two non-identical subunits with molecular masses of 35 and 21 kda. the isoelectric point of the purified enzyme was 5.3. the enzyme had an optimal ph of 5.5 and an optimal temperature of ... | 1998 | 9756463 |
| an integrated process for the production of toxic catechols from toxic phenols based on a designer biocatalyst. | we describe the biocatalytic production of 3-phenylcatechol from 2-phenylphenol with the whole cell biocatalyst escherichia coli jm101 (phbp461). the recombinant produces 2-hydroxybiphenyl 3-monooxygenase, an enzyme from pseudomonas azelaica hbp1. this enzyme introduces a hydroxyl-group at the c3-position of a variety of 2-substituted phenols, such as 2-phenylphenol. this permits the biocatalytic production of 3-substituted catechols, which are difficult to synthesize chemically. both 2-phenylph ... | 1999 | 9951522 |
| production of polyhydroxyalkanoates by pseudomonas nitroreducens. | a strain coded as 1.2343 was isolated from oil-contaminated soil in an oil-field in north china tianjian city and it was identified as pseudomonas nitroreducens. the strain demonstrated some unusual ability to synthesize polyhydroxybutyrate (phb) homopolymer from medium-chain-length (mcl) fatty acids including hexanoate and octanoate. while polyhydroxyalkanoates (pha) consisting of mcl hydroxyalkanoate (ha) monomers such as hydroxyoctanoate (ho) and hydroxydecanoate (hd) were the major compositi ... | 1999 | 10510722 |
| catalytic mechanism of 2-hydroxybiphenyl 3-monooxygenase, a flavoprotein from pseudomonas azelaica hbp1. | 2-hydroxybiphenyl 3-monooxygenase (ec 1.14.13.44) from pseudomonas azelaica hbp1 is an fad-dependent aromatic hydroxylase that catalyzes the conversion of 2-hydroxybiphenyl to 2, 3-dihydroxybiphenyl in the presence of nadh and oxygen. the catalytic mechanism of this three-substrate reaction was investigated at 7 degrees c by stopped-flow absorption spectroscopy. various individual steps associated with catalysis were readily observed at ph 7.5, the optimum ph for enzyme turnover. anaerobic reduc ... | 1999 | 10559214 |
| hbpr, a new member of the xylr/dmpr subclass within the ntrc family of bacterial transcriptional activators, regulates expression of 2-hydroxybiphenyl metabolism in pseudomonas azelaica hbp1. | the regulation of 2-hydroxybiphenyl and 2,2'-dihydroxybiphenyl degradation in pseudomonas azelaica is mediated by the regulatory gene, hbpr. the hbpr gene encodes a 63-kda protein belonging to the ntrc family of prokaryotic transcriptional activators and having the highest homology to members of the xylr/dmpr subclass. disruption of the hbpr gene in p. azelaica and complementation in trans showed that the hbpr protein was the key regulator for 2-hydroxybiphenyl metabolism. induction experiments ... | 2000 | 10629187 |
| transcriptional organization and dynamic expression of the hbpcad genes, which encode the first three enzymes for 2-hydroxybiphenyl degradation in pseudomonas azelaica hbp1. | pseudomonas azelaica hbp1 degrades the toxic substance 2-hydroxybiphenyl (2-hbp) by means of three enzymes that are encoded by structural genes hbpc, hbpa, and hbpd. these three genes form a small noncontiguous cluster. their expression is activated by the product of regulatory gene hbpr, which is located directly upstream of the hbpcad genes. the hbpr protein is a transcription activator and belongs to the so-called xylr/dmpr subclass within the ntrc family of transcriptional activators. transc ... | 2001 | 11114926 |
| pcr cloning of type ii polyhydroxyalkanoate biosynthesis genes from two pseudomonas strains. | two polyhydroxyalkanoate synthase genes, phac1 from pseudomonas pseudoalcaligenes hbq06 and phac2 from pseudomonas nitroreducens 0802, were cloned using a pcr cloning strategy based on the type ii pha loci property of pseudomonas strains. the complete open reading frames (orfs) of phac1 (p. nitroreducens hbq06) and phac2 (p. nitroreducens 0802) were identified from the pcr products. using the sequence information, the complete pha synthase genes were pcr cloned directly from the genomic dna and ... | 2001 | 11430409 |
| unusual location of two nearby pairs of upstream activating sequences for hbpr, the main regulatory protein for the 2-hydroxybiphenyl degradation pathway of "pseudomonas azelaica" hbp1. | "pseudomonas azelaica" hbp1 degrades 2-hydroxybiphenyl (2-hbp) and 2,2'-dihbp by employing a meta-cleavage pathway encoded by the hbpcad genes. the regulatory gene hbpr, located directly upstream of the hbpcad genes and oriented in the opposite direction, encodes a transcription activator protein belonging to the so-called xylr/dmpr subclass within the ntrc family. hbpr activates transcription from two separate sigma(54)-dependent promoters upstream of the hbpc and the hbpd genes, in the presenc ... | 2001 | 11495995 |
| changing the substrate reactivity of 2-hydroxybiphenyl 3-monooxygenase from pseudomonas azelaica hbp1 by directed evolution. | the substrate reactivity of the flavoenzyme 2-hydroxybiphenyl 3-monooxygenase (ec, hbpa) was changed by directed evolution using error-prone pcr. in situ screening of mutant libraries resulted in the identification of proteins with increased activity towards 2-tert-butylphenol and guaiacol (2-methoxyphenol). one enzyme variant contained amino acid substitutions v368a/l417f, which were inserted by two rounds of mutagenesis. the double replacement improved the efficiency of substrate hydroxylation ... | 2002 | 11733527 |
| identification and physical characterization of the hbpr binding sites of the hbpc and hbpd promoters. | pseudomonas azelaica hbp1 can use 2-hydroxybiphenyl (2-hbp) and 2,2'-dihydroxybiphenyl as sole carbon and energy sources by means of the hbp regulon. this regulon is composed of three genes, hbpca and hbpd, coding for enzymes of a meta-cleavage pathway and the hbpr gene, which codes for a xylr/dmpr-type transcription regulator. it was previously shown that hbpr activates transcription from two sigma(54)-dependent promoters, p(hbpc) and p(hbpd), in the presence of 2-hbp. in this study, by using g ... | 2002 | 12003931 |
| hydroxylation of indole by laboratory-evolved 2-hydroxybiphenyl 3-monooxygenase. | directed enzyme evolution of 2-hydroxybiphenyl 3-monooxygenase (hbpa; ec ) from pseudomonas azelaica hbp1 resulted in an enzyme variant (hbpa(ind)) that hydroxylates indole and indole derivatives such as hydroxyindoles and 5-bromoindole. the wild-type protein does not catalyze these reactions. hbpa(ind) contains amino acid substitutions d222v and v368a. the activity for indole hydroxylation was increased 18-fold in this variant. concomitantly, the k(d) value for indole decreased from 1.5 mm to 7 ... | 2002 | 12105208 |
| synthesis of 3-tert-butylcatechol by an engineered monooxygenase. | recombinant escherichia coli jm101 was used for the in vivo biocatalytic synthesis of 3-tert-butyl- catechol. the bacterial strain synthesized the laboratory-evolved variant hbpa(t2) of 2-hydroxybiphenyl 3-monooxygenase (hbpa, ec 1.14.13.44) from pseudomonas azelaica hbp1. the mutant enzyme hbpa(t2) is able to hydroxylate 2-tert-butylphenol to the corresponding catechol, a reaction that is not catalyzed by the wild-type enzyme. the biotransformation was performed in a 3-l bioreactor for 24 h. to ... | 2003 | 12514800 |
| pseudomonas koreensis sp. nov., pseudomonas umsongensis sp. nov. and pseudomonas jinjuensis sp. nov., novel species from farm soils in korea. | among pseudomonas strains isolated from korean agricultural soils, four strains (ps 9-14 group: ps 1-2, ps 1-10, ps 5-5 and ps 9-14t) from the suwon, goesan and samchok regions, three strains (ps 3-10 group: ps 2-22, ps 3-1 and ps 3-10t) from umsong region and four strains (pss 26 group: pss 14, pss 25, pss 26t and pss 27) from jinju region were identified as three independent groups on the basis of 16s rdna sequence analysis. while, on the basis of 16s rdna sequence analysis, ps 9-14t and ps 3- ... | 2003 | 12656147 |
| crystallization and preliminary x-ray analysis of native and selenomethionine 2-hydroxybiphenyl 3-monooxygenase. | 2-hydroxybiphenyl 3-monooxygenase (hbpa; ec 1.14.13.44) from pseudomonas azelaica hbp1 was produced in escherichia coli both as native and semet-labelled protein. the two enzymes were purified to homogeneity and crystallized by the hanging-drop vapour-diffusion method. the crystals belong to the monoclinic space group c2, with unit-cell parameters a = 108.6, b = 196.8, c = 79.3 a, beta = 97.7 degrees for the native protein and a = 108.3, b = 196.8, c = 79.0 a, beta = 97.8 degrees for semet hbpa. ... | 2003 | 12657798 |
| conversion of 2-fluoromuconate to cis-dienelactone by purified enzymes of rhodococcus opacus 1cp. | the present study describes the (19)f nuclear magnetic resonance analysis of the conversion of 3-halocatechols to lactones by purified chlorocatechol 1,2-dioxygenase (clca2), chloromuconate cycloisomerase (clcb2), and chloromuconolactone dehalogenase (clcf) from rhodococcus opacus 1cp grown on 2-chlorophenol. the 3-halocatechol substrates were produced from the corresponding 2-halophenols by either phenol hydroxylase from trichosporon cutaneum or 2-hydroxybiphenyl 3-mono-oxygenase from pseudomon ... | 2003 | 12957954 |
| dna-based methodologies for rapid detection, quantification, and species- or strain-level identification of respiratory pathogens (mycobacteria and pseudomonads) in metalworking fluids. | mycobacteria and pseudomonads occurring in modern metalworking fluids (mwf) have been implicated in occupational health hazards as causal agents for hypersensitivity pneumonitis (hp) and other respiratory illnesses in machine workers exposed to these fluids and their aerosols. unlike the conventional cultural and biochemical methods, which are often slow and ambiguous and detect only culturable cells, dna-based methods offer a time-saving alternative for reliable detection and identification of ... | 2003 | 14555451 |
| design of new promoters and of a dual-bioreporter based on cross-activation by the two regulatory proteins xylr and hbpr. | the hbpr protein is the sigma54-dependent transcription activator for 2-hydroxybiphenyl degradation in pseudomonas azelaica. the ability of hbpr and xylr, which share 35% amino acid sequence identity, to cross-activate the phbpc and pu promoters was investigated by determining hbpr- or xylr-mediated luciferase expression and by dna binding assays. xylr measurably activated the phbpc promoter in the presence of the effector m-xylene, both in escherichia coli and pseudomonas putida. hbpr weakly st ... | 2004 | 15479251 |
| characterization of hbpr binding by site-directed mutagenesis of its dna-binding site and by deletion of the effector domain. | in the presence of 2-hydroxybiphenyl, the enhancer binding protein, hbpr, activates the sigma54-dependent p(hbpc) promoter and controls the initial steps of 2-hydroxybiphenyl degradation in pseudomonas azelaica. in the activation process, an oligomeric hbpr complex of unknown subunit composition binds to an operator region containing two imperfect palindromic sequences. here, the hbpr-dna binding interactions were investigated by site-directed mutagenesis of the operator region and by dna-bindin ... | 2005 | 15794762 |
| specific identification of (r)-3-hydroxyacyl-acp: coa transacylase gene from pseudomonas and burkholderia strains by polymerase chain reaction. | polyhydroxyalkanoates (pha) were biodegradable thermoplastics. due to their broad applications, direct biosynthesis of pha from inexpensive substrates, such as carbohydrates, is actively pursued. it has been recently revealed that (r)-3-hydroxyacyl-acp: coa transacylase (phag) played an important role in this pathway. in this study, a polymerase chain reaction (pcr) protocol was developed for the rapid and specific identification of phag gene from various bacteria. using the pcr strategy, the co ... | 2005 | 15859323 |
| microbial demetallization of crude oil: nickel protoporphyrin disodium as a model organo-metallic substrate. | a soil isolate designated as ya-1 strain was selected for its ability to degrade nickel protoporphyrin disodium (nippds). the strain was capable of utilizing nippds as the sole source of carbon. this strain, a gram-negative aerobic rod, was identified as pseudomonas azelaica ya-1 based on the result of its 16s rrna analysis. product analyses by hplc showed that this strain can decompose the porphyrin ring to which a metal ion is bound. however, the use of whole bacterial cells cannot result in e ... | 2000 | 16232901 |
| growth factors, kinetics and biodegradation mechanism associated with pseudomonas nitroreducens tx1 grown on octylphenol polyethoxylates. | the growth properties and biodegradation mechanism of a gram-negative bacterium, pseudomonas nitroreducens tx1 that was able to grow on branched octylphenol polyethoxylates (opeo(n), average n=9.5) as the sole carbon source over a wide concentration range (1-100,000 mgl(-1)) were studied. analysis of growth factors indicated the highest specific growth rate (micro) of 0.53 h(-1) was obtained at an initial concentration of 5,000 mgl(-1) opeo(n). an optimal c/n ratio of 12 was obtained for (nh(4)) ... | 2006 | 16545517 |
| pseudomonas delhiensis sp. nov., from a fly ash dumping site of a thermal power plant. | a phenanthrene- and citronellol-degrading bacterium, strain rld-1(t), was isolated from the fly ash dumping site of a thermal power plant in delhi, india. the 16s rrna gene sequence indicated that this strain belongs to the genus pseudomonas; high levels of sequence similarity were found with respect to pseudomonas citronellolis dsm 50332(t) (98.9 %), pseudomonas jinjuensis dsm 16612(t) (97.6 %) and pseudomonas nitroreducens dsm 14399(t) (97.5 %). phylogenetic analysis based on 16s rrna gene seq ... | 2007 | 17329778 |
| pseudomonas knackmussii sp. nov. | the taxonomic position of pseudomonas sp. b13(t), isolated as a 3-chlorobenzoate-degrading organism and used for several groundbreaking studies on the enzymology and genetics of the degradative pathway for haloaromatic compounds, was studied in detail. the previously performed physiological studies, the detection of ubiquinone q-9, the polyamine pattern with putrescine and spermidine as major polyamines, a fatty acid profile with c(18 : 1)omega7c, summed feature 3 and c(16 : 0) as quantitatively ... | 2007 | 17329787 |
| characterization of 'pseudomonas azelaica' dsm 9128, leading to emended descriptions of pseudomonas citronellolis seubert 1960 (approved lists 1980) and pseudomonas nitroreducens iizuka and komagata 1964 (approved lists 1980), including pseudomonas multiresinivorans as its later heterotypic synonym. | polyphasic characterization of strain dsm 9128, described as 'pseudomonas azelaica' by janota-bassalik et al. [acta microbiol pol b 3, 143-153 (1971)], and four biochemically similar isolates was performed with the aim of validly publishing the name 'pseudomonas azelaica'. based on 16s rrna gene sequence analysis, dna-dna hybridization, fatty acid patterns and extensive biochemical testing, it was concluded that dsm 9128, two further strains and the type strains of pseudomonas nitroreducens and ... | 2007 | 17392224 |
| hydroxylated polychlorinated biphenyl detection based on a genetically engineered bioluminescent whole-cell sensing system. | the metabolites of polychlorinated biphenyls (pcbs), such as hydroxylated pcbs (oh-pcbs), have been identified as environmental contaminants. various studies have shown that some oh-pcbs can potentially contribute to health problems. detection of these compounds in environmental and biological samples could provide useful information about their levels and lead to a better understanding of their apparent toxicity. to that end, we have developed a whole-cell sensing system for the detection of oh ... | 2007 | 17602671 |
| metabolic characterization of newly isolated pseudomonas nitroreducens jin1 growing on eugenol and isoeugenol. | newly isolated soil bacterium strain jin1 was able to grow on both eugenol and isoeugenol each as sole source of carbon and energy. based on bacterial 16s rdna analysis, jin1 belongs to pseudomonas nitroreducens with a similarity of 98.92% (14/1297). p. nitroreducens jin1 was found to biotransform eugenol and isoeugenol to vanillin by different pathways. eugenol was biotransformed to vanillin through coniferyl alcohol and ferulic acid similarly to the pathway shown previously by pseudomonassp. h ... | 2007 | 17867641 |
| [isolation, idetification of 1,2, 4-trichlorobenzene-degrading strain pseudomonas nitroreducens j5-1 and cloning of chlorocatechol 1,2-dioxygenase gene]. | a bacterium capable of utilizing 1,2,4-trichlorobenzene as sole carbon source was isolated from the polluted soil sample. this baterium was identified as pseudomonas nitroreducens according to its physiological & biochemical analysis and its 16s rdna sequence (genbank accession no. ef107515). when the initial concentration of 1,2,4-tcb is 400 mg/l, j5-1 can achieve a maximum degradation rate of 90%. when the initial concentration of 1,2,4-tcb is 20 mg/l, the effect of degradation is the best. de ... | 2007 | 17926427 |
| description of a novel indole-oxidizing bacterium pseudomonas indoloxydans sp. nov., isolated from a pesticide-contaminated site. | a gram-negative, deep brown-pigmented gammaproteobacteria, strain ipl-1(t), capable of oxidizing indole was isolated from a lindane-contaminated site and subjected to a polyphasic taxonomic study. most of the physiological and biochemical properties, major fatty acids (c(18:1)omega7c, c(16:1)omega7c/iso c(15:0) 2oh and c(16:0)), estimated dna g+c content (67.2mol%) and 16s rrna gene sequence analysis showed that strain ipl-1(t) belonged to the genus pseudomonas. strain ipl-1(t) exhibited highest ... | 2008 | 18406094 |
| pseudomonas panipatensis sp. nov., isolated from an oil-contaminated site. | a gram-negative, motile, rod-shaped, non-sporulating, aerobic bacterial strain (esp-1(t)) was isolated from oil-contaminated soil of panipat oil refinery, india, and its taxonomic position was determined using a polyphasic approach. strain esp-1(t) grew in the presence of 2 % nacl at 30 degrees c and was characterized chemotaxonomically by having c(16 : 0) as the major fatty acid followed by c(17 : 0) cyclo and c(18 : 1)omega7c. phylogenetic analysis based on 16s rrna gene sequences showed that ... | 2008 | 18523175 |
| isolation and characterization of a novel simazine-degrading bacterium from agricultural soil of central chile, pseudomonas sp. mhp41. | s-triazine herbicides are used extensively in south america in agriculture and forestry. in this study, a bacterium designated as strain mhp41, capable of degrading simazine and atrazine, was isolated from agricultural soil in the quillota valley, central chile. strain mhp41 is able to grow in minimal medium, using simazine as the sole nitrogen source. in this medium, the bacterium exhibited a growth rate of mu=0.10 h(-1), yielding a high biomass of 4.2 x 10(8) cfu ml(-1). resting cells of strai ... | 2008 | 18647357 |
| [cloning and sequence analysis of 1,2,4-trichlorobenzene dioxygenase and dehydrogenase genes]. | pseudomonas nitroreducens j5-1 is able to use monochlorobenzene, 1,2-dichlorobenzene, 1,3-dichlorobenzene and 1,2,4-trichlorobenzene as sole carbon and energy sources, and it differs from those 1,2,4-trichlorobenzene degrading bacteria reported in substrate utilizing characters. pcr technique was used to amplify the genes of chlorobenzene dioxygenase and dehydrogenase of j5-1, and they were named as tcba and tcbb, respectively. homology analysis indicated that these genes and gene products were ... | 2008 | 18763518 |
| rhizosphere remediation of chlorpyrifos in mycorrhizospheric soil using ryegrass. | the potential of ryegrass for rhizosphere bioremediation of chlorpyrifos in mycorrhizal soil was investigated by the green house pot culture experiments. the pot cultured soil amended at initial chlorpyrifos concentration of 10mg/kg was observed to be degraded completely within 7 days where the rest amended concentrations (25-100mg/kg) decreased rapidly under the influence of ryegrass mycorrhizosphere as the incubation progressed till 28 days. this bioremediation of chlorpyrifos in soil is attri ... | 2009 | 19720454 |
| isolation, identification and characterization of soil microbes which degrade phenolic allelochemicals. | to isolate and characterize microbes in the soils containing high contents of phenolics and to dissolve the allelopathic inhibition of plants through microbial degradation. | 2010 | 19912433 |
| isoeugenol monooxygenase and its putative regulatory gene are located in the eugenol metabolic gene cluster in pseudomonas nitroreducens jin1. | the plant-derived phenylpropanoids eugenol and isoeugenol have been proposed as useful precursors for the production of natural vanillin. genes involved in the metabolism of eugenol and isoeugenol were clustered in region of about a 30 kb of pseudomonas nitroreducens jin1. two of the 23 orfs in this region, orfs 26 (iemr) and 27 (iem), were predicted to be involved in the conversion of isoeugenol to vanillin. the deduced amino acid sequence of isoeugenol monooxygenase (iem) of strain jin1 had 81 ... | 2010 | 20091296 |
| development of an autofluorescent pseudomonas nitroreducens with dehydrochlorinase activity for efficient mineralization of gamma-hexachlorocyclohexane (gamma-hch). | biodegradation or bioremediation is a more efficient and environmental friendly method for detoxification of hexachlorocyclohexane (hch) residues compared to physical and chemical methods. here, we report the functional expression of dehydrochlorinase (lina) and enhanced green fluorescent protein (egfp) in pseudomonas nitroreducens for efficient biodegradation of gamma-hch. the broad-host-range plasmid pvag33, harboring dehydrochlorinase gene (lina) and enhanced green fluorescent protein gene (e ... | 2010 | 20132847 |
| molecular cloning and characterization of γ-glutamyltranspeptidase from pseudomonas nitroreducens ifo12694. | γ-glutamyltranspeptidase from pseudomonas nitroreducens ifo12694 (pnggt) exhibited higher hydrolytic activity than transfer activity, as compared with other γ-glutamyltranspeptidases (ggts). pnggt showed little activity towards most of l-amino acids and towards glycyl-glycine, which is often used as a standard γ-glutamyl accepter in ggt transfer reactions. the preferred substrates for pnggt as a γ-glutamyl accepter were amines such as methylamine, ethylamine, and isopropylamine. | 2010 | 20834145 |
| why two are not enough: degradation of p-toluenesulfonate by a bacterial community from a pristine site in moorea, french polynesia. | in previous work, only one culture (strain ta12) from a pristine site was reported to utilize the xenobiotic compound p-toluenesulfonate (tsa) as a sole source of carbon and energy for aerobic growth. 'strain ta12' has now been recognized as a community of three bacteria: achromobacter xylosoxidans ta12-a, ensifer adhaerens ta12-b and pseudomonas nitroreducens ta12-c. achromobacter xylosoxidans ta12-a and e. adhaerens ta12-b were identified as the tsa degraders. these two organisms contain sever ... | 2011 | 21204940 |
| characterisation of the putative effector interaction site of the regulatory hbpr protein from pseudomonas azelaica by site-directed mutagenesis. | bacterial transcription activators of the xylr/dmpr subfamily exert their expression control via ¤â(54)-dependent rna polymerase upon stimulation by a chemical effector, typically an aromatic compound. where the chemical effector interacts with the transcription regulator protein to achieve activation is still largely unknown. here we focus on the hbpr protein from pseudomonas azelaica, which is a member of the xylr/dmpr subfamily and responds to biaromatic effectors such as 2-hydroxybiphenyl. w ... | 2011 | 21379585 |
| isolation of a gene responsible for the oxidation of trans-anethole to para-anisaldehyde by pseudomonas putida jyr-1 and its expression in escherichia coli. | a plasmid, pta163, in escherichia coli contained an approximately 34-kb gene fragment from pseudomonas putida jyr-1 that included the genes responsible for the metabolism of trans-anethole to protocatechuic acid. three tn5-disrupted open reading frame 10 (orf 10) mutants of plasmid pta163 lost their abilities to catalyze trans-anethole. heterologously expressed orf 10 (1,047 nucleotides [nt]) under a t7 promoter in e. coli catalyzed oxidative cleavage of a propenyl group of trans-anethole to an ... | 2012 | 22610435 |
| characterization of a mexab-oprm efflux system necessary for productive metabolism of pseudomonas azelaica hbp1 on 2-hydroxybiphenyl. | pseudomonas azelaica hbp1 is one of the few bacteria known to completely mineralize the biocide and toxic compound 2-hydroxybiphenyl (2-hbp), but the mechanisms of its tolerance to the toxicity are unknown. by transposon mutant analysis and screening for absence of growth on water saturating concentrations of 2-hbp (2.7 mm) we preferentially found insertions in three genes with high homology to the mexa, mexb, and oprm efflux system. mutants could grow at 2-hbp concentrations below 100 μm but at ... | 2013 | 23882265 |
| draft genome sequence of pseudomonas nitroreducens strain tx1, which degrades nonionic surfactants and estrogen-like alkylphenols. | pseudomonas nitroreducens tx1 atcc pta-6168 was isolated from rice field drainage in taiwan. the bacterium is of special interest because of its capability to use nonionic surfactants (alkylphenol polyethoxylates) and estrogen-like compounds (4-t-octylphenol and 4-nonylphenol) as a sole carbon source. this is the first report on the genome sequence of p. nitroreducens. | 2014 | 24482523 |
| enrichment and characterization of a bacterial culture that can degrade 4-aminopyridine. | the agrichemical 4-aminopyridine is used as a bird repellent in crop fields and has an epileptogenic action in a variety of animals, including man and mouse. 4-aminopyridine is biodegraded in the environment through an unknown mechanism. | 2013 | 23517195 |
| isolation and identification of cellulolytic bacteria from the gut of holotrichia parallela larvae (coleoptera: scarabaeidae). | in this study, 207 strains of aerobic and facultatively anaerobic cellulolytic bacteria were isolated from the gut of holotrichia parallela larvae. these bacterial isolates were assigned to 21 genotypes by amplified ribosomal dna restriction analysis (ardra). a partial 16s rdna sequence analysis and standard biochemical and physiological tests were used for the assignment of the 21 representative isolates. our results show that the cellulolytic bacterial community is dominated by the proteobacte ... | 2012 | 22489111 |
| sodium lauryl ether sulfate (sles) degradation by nitrate-reducing bacteria. | the surfactant sodium lauryl ether sulfate (sles) is widely used in the composition of detergents and frequently ends up in wastewater treatment plants (wwtps). while aerobic sles degradation is well studied, little is known about the fate of this compound in anoxic environments, such as denitrification tanks of wwtps, nor about the bacteria involved in the anoxic biodegradation. here, we used sles as sole carbon and energy source, at concentrations ranging from 50 to 1000 mg l(-1), to enrich an ... | 2017 | 28299401 |
| investigation, expression, and molecular modeling of orf2, a metagenomic lipolytic enzyme. | one clone exhibiting lipolytic activity was selected among 30 positives from a metagenomic library of a microbe consortium specialized in petroleum hydrocarbon degradation. from this clone, a sublibrary was constructed and a metagenome contig was assembled and analyzed using the orf finder; thus, it was possible to identify a potential orf that encodes a lipolytic enzyme, denoted orf2. this orf is composed of 1035-bp 345 amino acids and displayed 98 % identity with an alpha/beta hydrolase from p ... | 2015 | 25764223 |
| characterization of an isoeugenol monooxygenase (iem) from pseudomonas nitroreducens jin1 that transforms isoeugenol to vanillin. | the isoeugenol monooxygenase (iem) gene from pseudomonas nitroreducens jin1, responsible for the conversion of isoeugenol to vanillin, was cloned and overexpressed in escherichia coli. the purified iem had a predicted molecular mass of 54 kda. the v(max), k(m), and k(cat) values for it, using isoeugenol as substrate, were 4.2 µmol vanillin min(-1) mg(-1) of protein, 120 µm, and 3.8 s(-1), respectively. maximum substrate turnover for iem occurred at 30 °c and ph 9.0. an (18)oxygen-labeling experi ... | 2013 | 23391906 |
| transcriptional control of the isoeugenol monooxygenase of pseudomonas nitroreducens jin1 in escherichia coli. | vanillin is one of the most valuable compounds in the flavoring and fragrance industries, and many attempts to produce natural vanillin have been made in recent years. isoeugenol monooxygenase (iem) converts the phenylpropanoid compound isoeugenol to vanillin. in pseudomonas nitroreducens jin1, the positive regulatory protein iemr is divergently expressed from iem, and the promoter region is located between the genes. in this study, we investigated the transcriptional regulation of iem in escher ... | 2012 | 23047101 |
| pyrene biodegradation enhancement potential of lipopeptide biosurfactant produced by paenibacillus dendritiformis cn5 strain. | effect of biosurfactant on biodegradation of pyrene was studied using a microbial consortium predominantly composed of pseudomonas viridiflava (49.5%) and pseudomonas nitroreducens (32.5%) in a batch experiment containing lipopeptidic biosurfactant, produced by paenibacillus dendritiformis cn5 strain, and mineral salt medium. the results showed that the lipopeptide at 600 and 300mgl(-1) enhanced pyrene degradation to 83.5% and 67% respectively in 24days compared to 16% degradation in its absence ... | 2017 | 27627697 |
| pseudomonas nitritireducens sp. nov., a nitrite reduction bacterium isolated from wheat soil. | a gram-negative, non-mobile, polar single flagellum, rod-shaped bacterium wzbfd3-5a2(t) was isolated from a wheat soil subjected to herbicides for several years. cells of strain wzbfd3-5a2(t) grow optimally on luria-bertani agar medium at 30 °c in the presence of 0-4.0 % (w/v) nacl and ph 8.0. 16s rrna gene sequence analysis revealed that strain wzbfd3-5a2(t) belongs to the genus pseudomonas. physiological and biochemical tests supported the phylogenetic affiliation. strain wzbfd3-5a2(t) is clos ... | 2012 | 22918457 |
| genome of pseudomonas nitroreducens df05 from dioxin contaminated sediment downstream of the san jacinto river waste pits reveals a broad array of aromatic degradation gene determinants. | p. nitroreducens df05 is a gram negative, motile, aerobic, rod-shaped and psychrotrophic bacterium that was isolated from contaminated san jacinto river sediment near river terrace park in channelview, texas. the draft genome of strain df05 consists of a total of 192 contigs assembled at the scaffold level totaling 6,487,527 bp and encoding for 5862 functional proteins, 1116 of which are annotated as hypothetical proteins. the bacterial chromosome has a gc content of 65.15% and contains 22 rrna ... | 2017 | 28856100 |
| transposon mutagenesis identifies genes critical for growth of pseudomonas nitroreducens tx1 on octylphenol polyethoxylates. | pseudomonas nitroreducens tx1 is of special interest because of its ability to utilize 0.05% to 20% octylphenol polyethoxylates (opeon) as a sole source of carbon. in this study, a library containing 30,000 tn5-insertion mutants of the wild-type strain tx1 was constructed and screened for opeon utilization, and 93 mutants were found to be unable to grow on opeon in total, 42 separate disrupted genes were identified, and the proteins encoded by the genes were then classified into various categori ... | 2016 | 27590807 |
| a crystal structure of 2-hydroxybiphenyl 3-monooxygenase with bound substrate provides insights into the enzymatic mechanism. | 2-hydroxybiphenyl 3-monooxygenase (hbpa) is an fad dependent monooxygenase which catalyzes the ortho-hydroxylation of a broad range of 2-substituted phenols in the presence of nadh and molecular oxygen. we have determined the structure of hbpa from the soil bacterium pseudomonas azelaica hbp1 with bound 2-hydroxybiphenyl, as well as several variants, at a resolution of 2.3-2.5å to investigate structure function correlations of the enzyme. an observed hydrogen bond between 2-hydroxybiphenyl and h ... | 2015 | 26275805 |
| genome sequence of pseudomonas azelaica strain aramco j. | we report here the draft genome sequence of pseudomonas azelaica strain aramco j (7.3 mbp; gc content, 61.9%), one of the few bacteria that can completely mineralize different hydroxybiphenyls, e.g., 2-hydroxybiphenyl, 2,2'-dihydroxybiphenyl, and 3-hydroxybiphenyl. the findings obtained from its genome annotation suggest that this strain becomes a useful biocatalyst for aromatic bioconversions. | 2015 | 25744991 |
| structures of the apo and fad-bound forms of 2-hydroxybiphenyl 3-monooxygenase (hbpa) locate activity hotspots identified by using directed evolution. | the fad-dependent monooxygenase hbpa from pseudomonas azelaica hbp1 catalyses the hydroxylation of 2-hydroxybiphenyl (2hbp) to 2,3-dihydroxybiphenyl (23dhbp). hbpa has been used extensively as a model for studying flavoprotein hydroxylases under process conditions, and has also been subjected to directed-evolution experiments that altered its catalytic properties. the structure of hbpa has been determined in its apo and fad-complex forms to resolutions of 2.76 and 2.03 å, respectively. compariso ... | 2015 | 25737306 |
| pyrimidine nucleotide synthesis in pseudomonas nitroreducens and the regulatory role of pyrimidines. | control of pyrimidine biosynthesis in the commercially important, hydrocarbon-utilizing bacterium pseudomonas nitroreducens atcc 33634 was investigated. when glucose-grown wild-type cells were supplemented with uracil or orotic acid, the pyrimidine biosynthetic activities were depressed. pyrimidine limitation of glucose-grown cells of an orotate phosphoribosyltransferase mutant caused aspartate transcarbamoylase and dihydroorotase activities to increase by about 4-fold while the other enzyme act ... | 2014 | 24867376 |
| genome sequence of pseudomonas azelaica hbp1, which catabolizes 2-hydroxybiphenyl fungicide. | pseudomonas azelaica hbp1 (dsm 8897) is one of the few bacteria able to completely mineralize the 2-hydroxybiphenyl biocide. here, we report the draft genome sequence of this strain (7.4 mbp; g+c content, 63.5%) and the findings obtained from its genome annotation. | 2014 | 24526653 |
| examining chemical compound biodegradation at low concentrations through bacterial cell proliferation. | we show proof of principle for assessing compound biodegradation at 1-2 mg c per l by measuring microbial community growth over time with direct cell counting by flow cytometry. the concept is based on the assumption that the microbial community will increase in cell number through incorporation of carbon from the added test compound into new cells in the absence of (as much as possible) other assimilable carbon. we show on pure cultures of the bacterium pseudomonas azelaica that specific popula ... | 2013 | 23339277 |
| isolation and characterization of a lipopeptide bioemulsifier produced by pseudomonas nitroreducens tsb.mj10 isolated from a mangrove ecosystem. | pseudomonas nitroreducens tsb.mj10 exhibiting growth and bioemulsifier production with 0.5% sodium benzoate as the sole carbon source was isolated from a mangrove ecosystem in the vicinity of a petroleum pump. the bioemulsifier is a lipopeptide that is stable over a ph range of 5-11 and a temperature range of 20-90°c and showed emulsifying activity in the presence of relatively high nacl concentrations (up to 25%). the bioemulsifier formed stable emulsions with aliphatic (hexadecane, n-heptane, ... | 2012 | 22940327 |
| effects of carbon and nitrogen sources on rhamnolipid biosurfactant production by pseudomonas nitroreducens isolated from soil. | rhamnolipid biosurfactant production by pseudomonas nitroreducens isolated from petroleum-contaminated soil was investigated. the effects of carbon, nitrogen and carbon to nitrogen ratio on biosurfactant production were examined using mineral salts medium as the growth medium. the tenso-active properties (surface activity and critical micelle concentrations of the produced biosurfactant were also evaluated. the best carbon source, nitrogen source were glucose and sodium nitrate giving rhamnolipi ... | 2012 | 22805814 |
| γ-glutamyl transfer reactions by glutaminase from pseudomonas nitroreducens ifo 12694 and their application for the syntheses of theanine and γ-glutamylmethylamide. | in a mixture containing γ-glutamyl donor (donor) and γ-glutamyl acceptor (acceptor), the glutaminase of pseudomonas nitroreducens ifo 12694 simultaneously catalyzed a γ-glutamyl transfer reaction and hydrolysis of the donor. the variation of the activities responding to the concentration of glutathione and glycylglycine indicated that the enzyme might be classified in a group of glutaminases that shows hydrolysis prior to transfer reaction. on the other hand, the results with glutamine and ethyl ... | 1998 | 27396994 |
| purification and some properties of glutaminase from pseudomonas nitroreducens ifo 12694. | glutaminase (ec 3.5.1.2) was isolated from pseudomonas nitroreducens ifo 12694 grown on 0.6% sodium glutamate as a nitrogen source (325-fold purification, 13% yield). the molecular weight of the enzyme was estimated to be 40,000 by gel filtration and sds-gel electrophoresis. the enzyme hydro-lyzed glutamine optimally at ph 9, and its km was 6.5 mm. d-glutamine, γ-glutamyl p-nitroanilide, γ-glutamylmethylamide, γ-glutamylethylamide (theanine), and glutathione showed respectively 107, 85, 78, 74, ... | 1996 | 27299717 |