| fungitoxicity of chemical analogs with heartwood toxins. | trans-stilbene and tropolone as chemical analogs with naturally occurring fungitoxic heartwood compounds were studied with respect to their fungitoxic potency. while stilbene showed no fungitoxic activity towards the fungi aureobasidium pullulans var. melanogenum, penicillium glabrum, and trichoderma harzianum in the concentrations tested, the minimal inhibiting concentration of tropolone was 10(-3) m for penicillium glabrum and trichoderma harzianum, and 10(-5) m for aureobasidium pullulans var ... | 1998 | 9625794 |
| heavy oils, principally long-chain n-alkanes secreted by aureobasidium pullulans var. melanogenum strain p5 isolated from mangrove system. | in this study, the yeast strain p5 isolated from a mangrove system was identified to be a strain of aureobasidium pullulans var. melanogenum and was found to be able to secrete a large amount of heavy oil into medium. after optimization of the medium for heavy oil production and cell growth by the yeast strain p5, it was found that 120.0 g/l of glucose and 0.1 % corn steep liquor were the most suitable for heavy oil production. during 10-l fermentation, the yeast strain p5 produced 32.5 g/l of h ... | 2014 | 25038885 |
| genetic modification of the marine-isolated yeast aureobasidium melanogenum p16 for efficient pullulan production from inulin. | in this study, in order to directly and efficiently convert inulin into pullulan, the inu1 gene from kluyveromyces maximum km was integrated into the genomic dna and actively expressed in the high pullulan producer aureobasidium melanogenum p16 isolated from the mangrove ecosystem. after the ability to produce pullulan from inulin by different transformants was examined, it was found that the recombinant strain ei36, one of the transformants, produced 40.92 u/ml of inulinase activity while its w ... | 2015 | 25985744 |
| deterioration of expanded polystyrene caused by aureobasidium pullulans var. melanogenum. | an expanded-polystyrene factory located in northern buenos aires reported unusual dark spots causing esthetic damage in their production. a fungal strain forming black-olive colonies on extract malt agar medium was isolated from the damaged material and identified as aureobasidium pullullans var. melanogenum. this fungus is particularly known for its capacity to produce hydrolytic enzymes and a biodegradable extracellular polysaccharide known as pullulan, which is used in the manufacture of pack ... | 2015 | 26165967 |
| hydrocarbons, the advanced biofuels produced by different organisms, the evidence that alkanes in petroleum can be renewable. | it is generally regarded that the petroleum cannot be renewable. however, in recent years, it has been found that many marine cyanobacteria, some eubacteria, engineered escherichia coli, some endophytic fungi, engineered yeasts, some marine yeasts, plants, and insects can synthesize hydrocarbons with different carbon lengths. if the organisms, especially some native microorganisms and engineered bacteria and yeasts, can synthesize and secret a large amount of hydrocarbons within a short period, ... | 2015 | 26231137 |
| superficial phaeohyphomycosis caused by aureobasidium melanogenum mimicking tinea nigra in an immunocompetent patient and review of published reports. | aureobasidium pullulans is a ubiquitous black yeast-like fungus belonging to order dothideales. it was regarded as a contaminant, but is now considered a pathogen causing a wide range of human infections. we report a case of superficial phaeohyphomycosis in an immunocompetent patient with clinical presentations mimicking tinea nigra. on microscopic examination of lesion scales, multiple thick-walled, pigmented oval spores with septa were noted. a fungus with black mucoid colonies was repeatedly ... | 2016 | 26883514 |
| aureobasidium melanogenum: a native of dark biofinishes on oil treated wood. | the genus aureobasidium, which is known as a wood staining mould, has been detected on oil treated woods in the specific stain formation called biofinish. this biofinish is used to develop a new protective, self-healing and decorative biotreatment for wood. in order to understand and control biofinish formation on oil treated wood, the occurrence of different aureobasidium species on various wood surfaces was studied. phenotypic variability within aureobasidium strains presented limitations of m ... | 2016 | 26920754 |
| melanin production by a yeast strain xj5-1 of aureobasidium melanogenum isolated from the taklimakan desert and its role in the yeast survival in stress environments. | the yeast strain xj5-1 isolated from the taklimakan desert soil was identified to be a strain of aureobasdium melanogenum and could produce a large amount of melanin when it was grown in the pda medium, but its melanin biosynthesis and expression of the pks gene responsible for the melanin biosynthesis was significantly repressed in the presence of (nh4)2so4. however, a. melanogenum p5 strain isolated from a mangrove ecosystem grown in both the presence and the absence of (nh4)2so4 did not produ ... | 2016 | 27290725 |
| cloning, expression, and characterization of aureobasidium melanogenum lipase in pichia pastoris. | cdna of aureobasidium melanogenum lipase comprises 1254 bp encoding 417 amino acids, whereas genomic dna of lipase comprises 1311 bp with one intron (57 bp). the lipase gene contains a putative signal peptide encoding 26 amino acids. the a. melanogenum lipase gene was successfully expressed in pichia pastoris. recombinant lipase in an inducible expression system showed the highest lipase activity of 3.8 u/ml after six days of 2% v/v methanol induction. the molecular mass of purified recombinant ... | 2016 | 27427953 |
| crea is directly involved in pullulan biosynthesis and regulation of aureobasidium melanogenum p16. | aureobasidium melanogenum p16 is a high pullulan-producing yeast. however, glucose repression on its pullulan biosynthesis must be relieved. after the gene encoding a glucose repressor was cloned, characterized and analyzed, it was found that the repressor belonged to one member of the crea in filamentous fungi, not to one member of the mig1 in yeasts. after the crea gene was fully removed from the yeast strain p16, the glucose repression in the disruptant dg41 was relieved. at the same time, th ... | 2016 | 27628927 |
| both a pks and a pptase are involved in melanin biosynthesis and regulation of aureobasidium melanogenum xj5-1 isolated from the taklimakan desert. | a pks1 gene responsible for the melanin biosynthesis and a npg1 gene in aureobasidium melanogenum xj5-1 were cloned and characterized. an orf of the pks1 gene encoding a protein with 2165 amino acids contained 6495bp while an orf of the npg1 gene encoding a protein with 340 amino acids had 1076bp. after analysis of their promoters, it was found that expression of both the pks1 gene and the npg1 gene was repressed by nitrogen sources and glucose, respectively. the pks deduced from the cloned gene ... | 2017 | 27845206 |
| a glycosyltransferase gene responsible for pullulan biosynthesis in aureobasidium melanogenum p16. | in this study, one of the glucosyltransferase genes for pullulan production was cloned from aureobasidum melanogenum p16 and charaterized. it was found that the ugt1 gene had 4774bp with four introns (47, 52, 54 and 46bp). the n-terminal part of the protein displayed a conserved sequence controlling both sugar donor and accepter for substrate specificity whereas its c-terminal part carried a dxd motif that coordinated donor sugar binding. after complete removal of the gene ugt1, the mutant 1152- ... | 2017 | 27889342 |
| aureobasidium pullulans var. melanigenum fungemia in a pediatric patient. | this report describes a chronically ill child who presented with high fever and was diagnosed with catheter-related sepsis. aureobasidium pullulans variety melanigenum, a dematiaceous fungus that rarely causes opportunistic infections, was recovered from multiple blood cultures. antifungal susceptibilities were performed and the minimum inhibitory concentration (mic) for fluconazole was 64 mg/l, suggestive of fluconazole resistance. the patient made a full recovery after removal of the catheter ... | 2011 | 20513170 |
| yeasts and yeast-like fungi associated with tree bark: diversity and identification of yeasts producing extracellular endoxylanases. | a total of 239 yeast strains was isolated from 52 tree bark samples of the medaram and srisailam forest areas of andhra pradesh, india. based on analysis of d1/d2 domain sequence of 26s rrna gene, 114 strains were identified as ascomycetous; 107 strains were identified as basidiomycetous yeasts; and 18 strains were identified as yeast-like fungi. among the ascomycetous yeasts, 51% were identified as members of the genus pichia, and the remaining 49% included species belonging to the genera clavi ... | 2008 | 18219522 |
| acidophilic xylanase from aureobasidium pullulans: efficient expression and secretion in pichia pastoris and mutational analysis. | a yeast-like fungus aureobasidium pullulans var. melanigenum strain atcc 20524 produces an extracellular acidophilic endo-1,4-beta-xylanase with an optimum ph of 2.0 [ohta et al., j. biosci. bioeng., 92, 262-270 (2001)]. the xyni cdna encoding the precursor protein (xyni) was expressed in the methylotrophic yeast pichia pastoris under the control of the alcohol oxidase i gene promoter. the 34 amino acid prepro-signal peptide of the a. pullulans xyni directed the efficient secretion of 178 mg of ... | 2004 | 16233716 |
| purification and characterization of an acidophilic xylanase from aureobasidium pullulans var. melanigenum and sequence analysis of the encoding gene. | an extracellular endo-1,4-beta-xylanase was purified from the culture supernatant of aureobasidium pullulans var. melanigenum (atcc 20524) grown on oat-spelt xylan. the purified enzyme showed a single band on sds-polyacrylamide gel electrophoresis with an apparent m(r) of 24 kda and had an isoelectric point of 6.7. xylanase activity was optimal at ph 2.0 and 50 degrees c. the genomic dna and cdnas encoding this protein were cloned and sequenced. southern blot analysis indicated that the xylanase ... | 2001 | 16233094 |
| disseminated nosocomial fungal infection by aureobasidium pullulans var. melanigenum: a case report. | we report on a rare case of disseminated nosocomial fungal infection due to aureobasidium pullulans var. melanigenum in a severely traumatized patient. repeated blood and urine cultures yielded multicellular filamentous hyphal structures of varying size accompanied by budding yeast-like-cells of ellipsoidal morphology. the patient became asymptomatic after fluconazole therapy. | 2003 | 12958301 |
| pharmacological profile of hs-142-1, a novel nonpeptide atrial natriuretic peptide antagonist of microbial origin. i. selective inhibition of the actions of natriuretic peptides in anesthetized rats. | hs-142-1, a novel polysaccharide, isolated from the culture broth of aureobasidium pullulans var. melanigenum, has been found to inhibit selectively the binding of [125i]atrial natriuretic peptide (anp) to the guanylyl cyclase-linked anp receptor (anp-b receptor) and production of cyclic gmp by anp. the effect of this compound on renal and vascular actions evoked by exogenously administered natriuretic peptides was examined in anesthetized rats. the increase in urine flow and in the urinary excr ... | 1992 | 1346647 |