| agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. | two strains of a new gram-positive coryneform bacterium isolated from soil and from a sandstone surface are described. strain 2002-39/1t (t = type strain) is a coccoid, nonmotile, non-acid-fast, microaerophilic organism. the menaquinones of this strain are mk-12 and mk-11, and the main components of the whole-cell sugars are glucose and rhamnose. no mycolic acids are present. the g+c content of the dna is 74 mol%. comparative 16s ribosomal dna studies and a cell wall analysis revealed that this ... | 1996 | 8573501 |
| agrococcus citreus sp. nov., isolated from a medieval wall painting of the chapel of castle herberstein (austria). | a bacterial strain, d-1/1at, isolated from a medieval wall painting of the chapel of herberstein (styria, austria) was characterized by a polyphasic approach. strain d-1/1at shared 98.1% 16s rrna sequence similarity to agrococcus jenensis. the chemotaxonomic characteristics including polar lipid pattern, whole cell sugars, quinone system, polyamine pattern, cell wall composition and fatty acid profile were in good agreement with those of agrococcus jenensis. the g+c content of the dna was determ ... | 1999 | 10425775 |
| agrococcus baldri sp. nov., isolated from the air in the 'virgilkapelle' in vienna. | five coccoid, gram-positive strains were isolated from the air of the 'virgilkapelle' in vienna. a representative of these five strains, v-108t, shared 99.0 and 98.4% 16s rdna sequence similarity, respectively, with agrococcus jenensis dsm 9580 and agrococcus citreus dsm 12453t. colonies of the five strains were white when grown in the dark and turned yellow in the light. the strains displayed highly similar biochemical and physiological characteristics and showed only small differences in their ... | 2002 | 12148630 |
| bacterial diversity in a nonsaline alkaline environment: heterotrophic aerobic populations. | heterotrophic populations were isolated and characterized from an alkaline groundwater environment generated by active serpentinization, which results in a ca(oh)2-enriched, extremely diluted groundwater with ph 11.4. one hundred eighty-five strains were isolated in different media at different ph values during two sampling periods. to assess the degree of diversity present in the environment and to select representative strains for further characterization of the populations, we screened the is ... | 2004 | 15574939 |
| microcella putealis gen. nov., sp. nov., a gram-positive alkaliphilic bacterium isolated from a nonsaline alkaline groundwater. | three gram-positive bacteria designated cv-2t, cv-40 and ac-30 were isolated from a highly alkaline groundwater environment (ph 11.4). these organisms formed very small rod-shaped cells, are aerobic, non-spore-forming, catalase-positive, oxidase-negative, with an optimum growth temperature of 35 degrees c and optimum ph value of growth between 8.5 and 9.0. the strains possessed a novel b-type cell-wall peptidoglycan structure with lysine as the diamino acid; the major respiratory quinones were m ... | 2005 | 16104348 |
| surface microflora of four smear-ripened cheeses. | the microbial composition of smear-ripened cheeses is not very clear. a total of 194 bacterial isolates and 187 yeast isolates from the surfaces of four irish farmhouse smear-ripened cheeses were identified at the midpoint of ripening using pulsed-field gel electrophoresis (pfge), repetitive sequence-based pcr, and 16s rrna gene sequencing for identifying and typing the bacteria and fourier transform infrared spectroscopy and mitochondrial dna restriction fragment length polymorphism (mtdna rflp ... | 2005 | 16269673 |
| agrococcus lahaulensis sp. nov., isolated from a cold desert of the indian himalayas. | the taxonomic position of a lemon-yellow-pigmented actinobacterium, strain k22-21(t), isolated from a soil sample from lahaul-spiti valley in the indian himalayas, was determined using a polyphasic approach. the strain had phenotypic and chemical properties that were consistent with its classification in the genus agrococcus. alignment of the 16s rrna gene sequence of strain k22-21(t) with sequences from agrococcus jenensis dsm 9580(t), agrococcus baldri dsm 14215(t) and agrococcus citreus dsm 1 ... | 2006 | 16902012 |
| agrococcus casei sp. nov., isolated from the surfaces of smear-ripened cheeses. | seven gram-positive, coryneform bacteria with virtually identical whole-organism protein patterns were isolated from the surface of smear-ripened cheeses. representatives of these strains were the subject of a polyphasic study designed to establish their taxonomic status. the organisms formed a distinct branch in the microbacteriaceae 16s rrna gene tree and were most closely related to members of the genus agrococcus, sharing sequence similarities of 95.4-98.7 %. the chemotaxonomic profiles of t ... | 2007 | 17220448 |
| gene sequence phylogenies of the family microbacteriaceae. | the type strains of 32 species of 13 genera of the family microbacteriaceae were analysed with respect to gene-coding phylogeny for dna gyrase subunit b (gyrb), rna-polymerase subunit b (rpob), recombinase a (reca), and polyphosphate kinase (ppk). the resulting gene trees were compared with the 16s rrna gene phylogeny of the same strains. the topology of neighbour-joining and maximum parsimony phylogenetic trees, based on nucleic-acid sequences and protein sequences of housekeeping genes, differ ... | 2007 | 17551787 |
| [microbial diversity in scorpion intestine (buthus martensii karsch)]. | scorpion is an important officinal animal, and has a high nutritional value. in this study, the culture-independent and culture-dependent methods were used to investigate the microbial diversity in the scorpion's intestine. results based on culture-independent method showed the bacteria to be related to alpha, beta, gamma-proteobacteria. bacteria isolated by the culture-dependent method were high g + c, gram-positive bacteria. the genera enterobacter, serratia and ochrobactrum were detected by b ... | 2007 | 18062268 |
| agrococcus jejuensis sp. nov., isolated from dried seaweed. | a novel marine actinomycete, designated strain ssw1-48(t), was isolated from a dried seaweed sample collected from a beach in jeju, republic of korea. cells of the organism were non-motile, non-endospore-forming, gram-positive and rod-shaped. colonies were circular, smooth, translucent and yellow-coloured. the diagnostic diamino acid in the peptidoglycan was 2,4-diaminobutyric acid and the murein was of the acetyl type. mycolic acids were absent. the predominant menaquinones were mk-10 and mk-9. ... | 2008 | 18842844 |
| agrococcus versicolor sp. nov., an actinobacterium associated with the phyllosphere of potato plants. | the taxonomic position of a group of actinobacterial strains isolated from the phyllosphere of potato plants was investigated by using a polyphasic approach. although the similarity values for their 16s rrna gene sequences suggested an intermediate position between microbacterium and agrococcus, the phylogenetic tree demonstrated a clear clustering of the representative strain, k 114/01(t), within the genus agrococcus. the presence of 2,4-diaminobutyric acid as the diagnostic diamino acid in the ... | 2008 | 19060068 |
| agrococcus terreus sp. nov. and micrococcus terreus sp. nov., isolated from forest soil. | two bacterial strains, dng5t and v3m1t, isolated from forest soil of the changbai mountains in china, were characterized using a polyphasic approach. analysis of their 16s rrna gene sequences indicated that strains dng5t and v3m1t were phylogenetically related to members of the genus agrococcus (96.0-98.4% similarity) and micrococcus (96.7-98.0% similarity), respectively, within the order actinomycetales. strains dng5t and v3m1t were gram-stain-positive and strictly aerobic and formed yellow col ... | 2010 | 19783614 |
| population dynamics of two antilisterial cheese surface consortia revealed by temporal temperature gradient gel electrophoresis. | surface contamination of smear cheese by listeria spp. is of major concern for the industry. complex smear ecosystems have been shown to harbor antilisterial potential but the microorganisms and mechanisms involved in the inhibition mostly remain unclear, and are likely related to complex interactions than to production of single antimicrobial compounds. bacterial biodiversity and population dynamics of complex smear ecosystems exhibiting antilisterial properties in situ were investigated by tem ... | 2010 | 20222967 |
| agrococcus carbonis sp. nov., isolated from soil of a coal mine. | an actinobacterial strain, designated g4(t), isolated from a coal mine was subjected to polyphasic taxonomic characterization. cells were gram-stain-positive, yellow-pigmented, non-motile and non-spore-forming cocci. this organism possessed a type b peptidoglycan with diaminobutyric acid as diagnostic diamino acid. the major respiratory quinones were mk-9, mk-10 and mk-11. the major fatty acids were anteiso-c(15 : 0) (41.6 %) and anteiso-c(17 : 0) (32.8 %). the predominant cellular polar lipids ... | 2010 | 20601492 |
| surface microbial consortia from livarot, a french smear-ripened cheese. | the surface microflora (902 isolates) of livarot cheeses from three dairies was investigated during ripening. yeasts were mainly identified by fourier transform infrared spectroscopy. geotrichum candidum was the dominating yeast among 10 species. bacteria were identified using biotype 100 strips, dereplicated by repetitive extragenic palindromic pcr (rep-pcr); 156 representative strains were identified by either box-pcr or (gtg)(5)-pcr, and when appropriate by 16s rdna sequencing and sds-page an ... | 2011 | 21815832 |
| changes in the bacterial community structure and diversity during bamboo retting. | microbial retting is a critical step in obtaining fiber bundles from bamboo culm using indigenous microorganisms. a cultivation-independent technique for monitoring the changes in bacteria community during bamboo retting was applied in this work. this technique involves genetic profiling of pcr-amplified small-subunit rrna and the single-strand conformation polymorphism (sscp) gel analysis of the pcr-amplified 16s rdna fragments. the study revealed that both the structure and the diversity of in ... | 2011 | 21695788 |