| characterisation of lactic acid bacteria isolated from naturally fermented greek dry salami. | a total of 348 lactic acid bacteria isolated from five batches of naturally fermented dry salami at various stages of ripening were characterised. the majority of the strains were assigned to two main phylogenetic groups of species: (i) the psychrotrophic, formerly called atypical, meat streptobacteria (169 strains) and (ii) a new genus weissella (120), which was recently proposed (collins et al., 1993) to include leuconostoc paramesenteroides and some other closely related species. meat strepto ... | 1994 | 7848780 |
| taxonomic studies on some leuconostoc-like organisms from fermented sausages: description of a new genus weissella for the leuconostoc paramesenteroides group of species. | taxonomic studies were performed on some unknown leuconostoc-like organisms from fermented greek sausage. comparative 16s rrna sequence analysis showed the unidentified organisms represent a new line within the leuconostoc paramesenteroides group of species. on the basis of the results of this and earlier phylogenetic investigations, it is proposed that leuconostoc paramesenteroides and related species be reclassified in a new genus weissella. in addition a new species, weissella hellenica, is p ... | 1993 | 8294308 |
| 16s-23s rdna intergenic sequences indicate that leuconostoc oenos is phylogenetically homogeneous. | the study of the intra-specific phylogenetic structure of leuconostoc oenos is essential to understand the participation of several strains in malo-lactic fermentation (mlf). rflp of the pcr-amplified 16s-23s rdna intergenic spacer region (isr) was performed in leuc. oenos and other related species. the rflp patterns with seven endonucleases were identical for the 37 leuc. oenos strains, but differed from those obtained for all other species tested. this method could provide an invaluable insigh ... | 1996 | 8760923 |
| rrna gene restriction patterns as a characterization tool for lactobacillus sake strains producing ropy slime. | the rrna gene restriction patterns (ribotypes) of 69 ropy slime producing lactobacillus sake strains isolated mainly from vacuum-packaged meat products of ten meat plants were determined. ribotypes were compared to the corresponding patterns of non-ropy l. sake strains, and also to other species of the genus lactobacillus, carnobacterium and weissella associated with meat products. ropy slime-producing l. sake strains were divided into four characteristic groups corresponding to the phenotypic c ... | 1996 | 8854182 |
| analysis of the beta' subunit of dna-dependent rna polymerase does not support the hypothesis inferred from 16s rrna analysis that oenococcus oeni (formerly leuconostoc oenos) is a tachytelic (fast-evolving) bacterium. | rrna sequencing has shown that leuconostocs comprise three distinct phylogenetic lineages which have been designated separate genera (viz., the genera leuconostoc sensu stricto, oenococcus, and weissella). in addition, the 16s rrna line formed by oenococcus oeni (formerly leuconostoc oenos) is exceptionally long; this fact, together with variations in the compositions of conserved positions in the 16s rrna, has led to the hypothesis (d. yang and c. r. woese, syst. appl. microbiol. 12:145-149, 19 ... | 1996 | 8863429 |
| bacterial spoilage of meat and cured meat products. | the influence of environmental factors (product composition and storage conditions) on the selection, growth rate and metabolic activity of the bacterial flora is presented for meat (pork and beef) and cooked, cured meat products. the predominant bacteria associated with spoilage of refrigerated beef and pork, are brochothrix thermosphacta, carnobacterium spp., enterobacteriaceae, lactobacillus spp., leuconostoc spp., pseudomonas spp. and shewanella putrefaciens. the main defects in meat are off ... | 1996 | 8913812 |
| lactic acid bacteria of foods and their current taxonomy. | application of molecular genetic techniques to determine the relatedness of food-associated lactic acid bacteria has resulted in significant changes in their taxonomic classification. during the 1980s the genus streptococcus was separated into the three genera enterococcus, lactococcus and streptococcus. the lactic acid bacteria associated with foods now include species of the genera carnobacterium, enterococcus, lactobacillus, lactococcus, leuconostoc, oenococcus, pediococcus, streptococcus, te ... | 1997 | 9168311 |
| characterization of strains of leuconostoc mesenteroides by analysis of soluble whole-cell protein pattern, dna fingerprinting and restriction of ribosomal dna. | of 215 leuconostocs isolated from field grass, natural whey cultures and water-buffalo milk, 178 were identified as leuconostoc mesenteroides ssp. mesenteroides while 37 strains could not be identified. biochemical characterization allowed seven groups to be defined. representative strains of each group and different habitat and nine reference strains were selected for further analyses. protein profiles appeared suitable for species discrimination, but did not differentiate between the three sub ... | 1997 | 9172399 |
| multiple bacteriocin production by leuconostoc mesenteroides ta33a and other leuconostoc/weissella strains. | leuconostoc (lc.) mesenteroides ta33a produced three bacteriocins with different inhibitory activity spectra. bacteriocins were purified by adsorption/desorption from producer cells and reverse phase high-performance liquid chromatography. leucocin c-ta33a, a novel bacteriocin with a predicted molecular mass of 4598 da, inhibited listeria and other lactic acid bacteria (lab). leucocin b-ta33a has a predicted molecular mass of 3466 da, with activity against leuconostoc/weissella (w.) strains, and ... | 1997 | 9353216 |
| influence of lactobacillus spp. from an inoculant and of weissella and leuconostoc spp. from forage crops on silage fermentation | lactobacillus spp. from an inoculant and weissella and leuconostoc spp. from forage crops were characterized, and their influence on silage fermentation was studied. forty-two lactic acid-producing cocci were obtained from forage crops and grasses. all isolates were gram-positive, catalase-negative cocci that produced gas from glucose, and produced more than 90% of their lactate in the d-isomer form. these isolates were divided into groups a and b by sugar fermentation patterns. two representati ... | 1998 | 9687461 |
| usefulness of rapid gc analysis of cellular fatty acids for distinguishing weissella viridescens, weissella paramesenteroides, weissella hellenica and some non-identifiable, arginine-negative weissella strains of meat origin. | in an attempt to differentiate between weissella viridescens, w. paramesenteroides, w. hellenica and some atypical arginine-negative weissella isolates from meats, their cellular fatty acid composition was determined by a rapid gc method. results showed that w. viridescens synthesized eicosenoic (n-c20:1) acid, while the other two species did not. meanwhile, unlike w. paramesenteroides, w. hellenica lacked cyclopropane fatty acids with 19 carbon atoms, i.e. dihydrosterculic or lactobacillic acid ... | 1998 | 9704112 |
| molecular characterization of the lactobacillus community in traditional processing of mozzarella cheese. | the natural lactobacillus community involved in traditional mozzarella cheese production has been investigated. the bacterial associations of whey, curd before stretching and mozzarella were analyzed using randomly amplified polymorphic dna (rapd) to follow growth kinetics, and 16s rdna sequencing to identify the taxonomical position of isolated strains. analysis of rapd fingerprints revealed that the lactobacillus community was composed of 13 different biotypes and the sequence analysis of 16s ... | 1998 | 9761338 |
| diversity of lactic acid bacteria associated with ducks. | the diversity of lactic acid bacteria occurring in the crop and intestinal content of ducks of different ages (5 to 34 days) was evaluated using the rapd-pcr technique and comparative sequence analysis of 16s rrna. cluster analysis derived from isolates grown on rogosa agar used for the enrichment of lactic acid bacteria, revealed at least twenty six different groups of organisms representing species, subspecies and strains belonging to streptococcus, enterococcus, pediococcus, weissella and lac ... | 1998 | 9924827 |
| identification of lactic acid bacteria from chili bo, a malaysian food ingredient. | ninety-two strains of lactic acid bacteria (lab) were isolated from a malaysian food ingredient, chili bo, stored for up to 25 days at 28 degreesc with no benzoic acid (product a) or with 7,000 mg of benzoic acid kg-1 (product b). the strains were divided into eight groups by traditional phenotypic tests. a total of 43 strains were selected for comparison of their sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) whole-cell protein patterns with a sds-page database of lab. iso ... | 1999 | 9925588 |
| lactobacillus perolens sp. nov., a soft drink spoilage bacterium. | lactic acid bacteria that are able to spoil soft drinks with low ph comprise a limited number of acidotolerant or acidophilic species of the genera lactobacillus, leuconostoc and weissella. various gram-positive rods causing turbidity and off-flavour were isolated from orange lemonades. physiological and biochemical studies including sds-page whole-cell protein analysis showed a homogeneous group of organisms. the 16s rrna gene sequence analysis of two representatives revealed that they formed a ... | 1999 | 10553287 |
| polyphasic study of the spatial distribution of microorganisms in mexican pozol, a fermented maize dough, demonstrates the need for cultivation-independent methods to investigate traditional fermentations. | the distribution of microorganisms in pozol balls, a fermented maize dough, was investigated by a polyphasic approach in which we used both culture-dependent and culture-independent methods, including microbial enumeration, fermentation product analysis, quantification of microbial taxa with 16s rrna-targeted oligonucleotide probes, determination of microbial fingerprints by denaturing gradient gel electrophoresis (dgge), and 16s ribosomal dna gene sequencing. our results demonstrate that dgge f ... | 1999 | 10584005 |
| improved screening procedure for biogenic amine production by lactic acid bacteria. | an improved screening plate method for the detection of amino acid decarboxylase-positive microorganisms (especially lactic acid bacteria) was developed. the suitability and detection level of the designed medium were quantitatively evaluated by confirmation of amine-forming capacity using an hplc procedure. the potential to produce the biogenic amines (ba) tyramine, histamine, putrescine, and cadaverine, was investigated in a wide number of lactic acid bacteria (lab) of different origin, includ ... | 1999 | 10598112 |
| the spoilage microflora of cured, cooked turkey breasts prepared commercially with or without smoking. | lactobacillus sakei subsp. carnosus was predominant in the spoilage flora of sliced, vacuum-packed, smoked, oven-cooked turkey breast fillets which developed mild, sour spoilage flavors after 4 weeks storage at 4 degrees c. in contrast, leuconostoc mesenteroides subsp. mesenteroides predominated in the spoilage flora of sliced, vacuum-packed, unsmoked, boiled turkey breast fillets from the same plant which were also stored at 4 degrees c. the spoilage flora of the unsmoked breasts grew faster th ... | 2000 | 10857539 |
| temperature-dependent variation in api 50 ch fermentation profiles of lactobacillus species. | api 50 ch fermentation profiles of 45 lactobacillus, one atopobium, and three weissella strains incubated at 30 degrees c and 37 degrees c were evaluated. atopobium uli and ten species of lactobacillus showed stable patterns despite the change in temperature. the rest of the type strains showed discrepancy between the two incubation temperatures: 18 strains lost, 12 additionally fermented another sugar, and 7 others fermented a different one in lieu. the variation was maximum in l. delbrueckii s ... | 2000 | 10919394 |
| molecular diversity of leuconostoc mesenteroides and leuconostoc citreum isolated from traditional french cheeses as revealed by rapd fingerprinting, 16s rdna sequencing and 16s rdna fragment amplification. | for a long time, the identification of the leuconostoc species has been limited by a lack of accurate biochemical and physiological tests. here, we use a combination of rapd, 16s rdna sequencing, and 16s rdna fragment amplification with specific primers to classify different leuconostocs at the species and strain level. we analysed the molecular diversity of a collection of 221 strains mainly isolated from traditional french cheeses. the majority of the strains were classified as leuconostoc mes ... | 2000 | 10930080 |
| lactobacillus acidipiscis sp. nov. and weissella thailandensis sp. nov., isolated from fermented fish in thailand. | eleven strains of homofermentative, rod-shaped lactic acid bacteria and five strains of heterofermentative, sphere-shaped lactic acid bacteria were isolated from fermented fish (pla-ra and pla-chom) in thailand. they were identified as new species and named lactobacillus acidipiscis sp. nov. and weissella thailandensis sp. nov., respectively, on the basis of phylogenetic analysis of the 16s rrna gene sequences, dna relatedness and phenotypic characteristics. the type strain of l. acidipiscis is ... | 2000 | 10939653 |
| evaluation of numerical analyses of rapd and api 50 ch patterns to differentiate lactobacillus plantarum, lact. fermentum, lact. rhamnosus, lact. sake, lact. parabuchneri, lact. gallinarum, lact. casei, weissella minor and related taxa isolated from kocho and tef. | the study was carried out to assess the agreement of api 50 ch fermentation data of food lactobacilli with their rapd profiles to determine whether the system could be used alone as a reliable taxonomic tool for this genus. | 2000 | 11123470 |
| phenotypic and molecular identification and clustering of lactic acid bacteria and yeasts from wheat (species triticum durum and triticum aestivum) sourdoughs of southern italy. | the microflora of 25 wheat sourdoughs from the apulia region, southern italy, was characterized. the sourdoughs were mainly produced from triticum durum wheat. the number of lactic acid bacteria and yeasts ranged from ca. log 7.5 to log 9.3 colony forming units (cfu)/g and from log 5.5 to log 8.4 cfu/g, respectively. about 38% of the 317 isolates of lactic acid bacteria were identified by conventional physiological and biochemical tests. phenotypic identification was confirmed by 16s rdna and 16 ... | 2001 | 11252516 |
| assessment of acid production by various human oral micro-organisms when palatinose or leucrose is utilized. | one promising way of reducing caries is by using sucrose substitutes in food, e.g., palatinose or leucrose. previous experiments addressing cariogenic potential of sucrose substitutes have focused mainly on streptococcus mutans. however, given the many other micro-organisms in the oral cavity, this study compared the acid production of 100 bacterial strains representing 44 different species, by batch fermentation in a test tube containing, as a sole carbohydrate source, glucose, sucrose, palatin ... | 2001 | 11269732 |
| behavior of variable v3 region from 16s rdna of lactic acid bacteria in denaturing gradient gel electrophoresis. | separation of amplified v3 region from 16s rdna by denaturing gradient gel electrophoresis (dgge) was tested as a tool for differentiation of lactic acid bacteria commonly isolated from food. variable v3 regions of 21 reference strains and 34 wild strains referred to species belonging to the genera pediococcus, enterococcus, lactococcus, lactobacillus, leuconostoc, weissella, and streptococcus were analyzed. dgge profiles obtained were species-specific for most of the cultures tested. moreover, ... | 2001 | 11270655 |
| weissella confusa (basonym: lactobacillus confusus) bacteremia: a case report. | infection with lactobacillus is rare, and only a handful of species have been identified as being clinically significant: lactobacillus casei, lactobacillus rhamnosus, and lactobacillus leichmannii. the literature contains one case report of bacteremia caused by weissella confusa (basonym: lactobacillus confusus), but the clinical significance of the infection was unclear. we describe a case of w. confusa bacteremia in a 46-year-old man with a history of abdominal aortic dissection and repair. t ... | 2001 | 11283096 |
| 16s rdna-based identification of bacteria from conjunctival swabs by pcr and dgge fingerprinting. | establishment of a new molecular biology technique for the identification of multiple bacteria from the ocular environment, which can be applied supplementarily to cultivation in cases of severe bacterial infections. | 2001 | 11328723 |
| detection of lactobacillus, pediococcus, leuconostoc, and weissella species in human feces by using group-specific pcr primers and denaturing gradient gel electrophoresis. | denaturing gradient gel electrophoresis (dgge) of dna fragments generated by pcr with 16s ribosomal dna-targeted group-specific primers was used to detect lactic acid bacteria (lab) of the genera lactobacillus, pediococcus, leuconostoc, and weissella in human feces. analysis of fecal samples of four subjects revealed individual profiles of dna fragments originating not only from species that have been described as intestinal inhabitants but also from characteristically food-associated bacteria s ... | 2001 | 11375166 |
| molecular diversity of lactobacillus spp. and other lactic acid bacteria in the human intestine as determined by specific amplification of 16s ribosomal dna. | a lactobacillus group-specific pcr primer, s-g-lab-0677-a-a-17, was developed to selectively amplify 16s ribosomal dna (rdna) from lactobacilli and related lactic acid bacteria, including members of the genera leuconostoc, pediococcus, and weissella: amplicons generated by pcr from a variety of gastrointestinal (gi) tract samples, including those originating from feces and cecum, resulted predominantly in lactobacillus-like sequences, of which ca. 28% were most similar to the 16s rdna of lactoba ... | 2002 | 11772617 |
| taxonomic study of weissella confusa and description of weissella cibaria sp. nov., detected in food and clinical samples. | a taxonomic study was conducted to clarify the relationships of two bacterial populations belonging to the genus weissella. a total of 39 strains originating mainly from malaysian foods (22 strains) and clinical samples from humans (9 strains) and animals (6 strains) were analysed using a polyphasic taxonomic approach. the methods included classical phenotyping, whole-cell protein electrophoresis, 16s and 23s rdna rflp (ribotyping), determination of 16s rdna sequence homologies and dna-dna reass ... | 2002 | 11837296 |
| weissella kimchii sp. nov., a novel lactic acid bacterium from kimchi. | a gram-positive, catalase-negative, non-sporulating, facultatively anaerobic, short rod-shaped bacterium, with cells measuring 0.3-0.5 x 1-2 microm and designated strain chj3t, was isolated from partially fermented kimchi, a traditional korean fermented vegetable food. the strain produced co2 gas, d-lactate from glucose and dextran from sucrose and hydrolysed aesculin and arginine. it also fermented n-acetylglucosamine, amygdalin, arbutin, cellobiose, d-fructose, galactose, beta-gentiobiose, glu ... | 2002 | 11931163 |
| isolation, identification and changes in the composition of lactic acid bacteria during the malting of two different barley cultivars. | malt has a complex microbial population, which changes as the malting process commences. little is known about the proliferation of lactic acid bacteria (lab) in each of the malting phases. in this study, we determined the number of lab present in the different phases of malting with clipper and prisma barley cultivars. the strains were identified to species level by using numerical analysis of total soluble cell protein patterns, rapd-pcr banding patterns and 16s rrna sequencing. the number of ... | 2002 | 12038579 |
| variations in the membrane fatty acid composition of resistant or susceptible leuconostoc or weissella strains in the presence or absence of mesenterocin 52a and mesenterocin 52b produced by leuconostoc mesenteroides subsp. mesenteroides fr52. | mesenterocins 52a (mes52a) and 52b (mes52b) are antimicrobial peptides produced by leuconostoc mesenteroides subsp. mesenteroides fr 52. mes52a is a class iia bacteriocin of lactic acid bacteria with a broad spectrum of activity. mes52b is an atypical class ii bacteriocin with a narrow spectrum of activity. four leuconostoc and weissella wild-type strains were selected for their susceptibility or insensitivity to these mesenterocins. four strains resistant to mes52a or mes52b were generated from ... | 2002 | 12039749 |
| weissella soli sp. nov., a lactic acid bacterium isolated from soil. | phylogenetic analysis of the 16s rrna gene of bacterial isolates from garden soil showed relatedness to weissella kandleri and weissella confusa. however, the sequences had notable differences, and dna-dna hybridizations confirmed that the isolates are separate from these two species. the isolates could be further distinguished from all previously described weissella species by electrophoretic analysis of whole-cell proteins, as well as by the results from different biochemical tests. the name w ... | 2002 | 12054246 |
| identification of weissella species by the genus-specific amplified ribosomal dna restriction analysis. | the weissella species have been increasingly isolated from many food and meat samples. for rapid and systematic identification of weissella species, an amplified ribosomal dna restriction analysis (ardra) has been developed. this method employs both polymerase chain reaction (pcr) and restriction pattern analysis: first, pcr using the genus-specific primers that were designed from 16s rdna sequence produces a 725-bp band only from weissella species and next, restriction analysis using mnli, msei ... | 2002 | 12076783 |
| microbial community dynamics during the scamorza altamurana cheese natural fermentation. | the growth dynamics of the natural microbial community responsible for the fermentation of scamorza altamurana, a typical southern italian cheese made using backslopping, was investigated applying a polyphasic approach combining 1) microbial enumeration with culture media, 2) randomly amplified polymorphic dna (rapd) fingerprinting of microbial communities, 3) sequencing of partial 16s ribosomal dna (rdna) genes, and 4) physiological tests. viable cell counts on different culture media showed th ... | 2002 | 12146469 |
| weissella koreensis sp. nov., isolated from kimchi. | a taxonomic study was carried out on two strains that came from kimchi, a traditional korean fermented-vegetable food. the dna g+c content of these strains was 37 mol %. both strains contained lys-ala-ser in the cell walls. on the basis of morphological, physiological and chemotaxonomic characteristics, together with data from 16s rdna sequence comparisons and dna-dna reassociation, it is proposed that these strains represent a novel species of the genus weissella, weissella koreensis sp. nov. t ... | 2002 | 12148637 |
| effect of weissella confusa strain pl9001 on the adherence and growth of helicobacter pylori. | viable and nonviable weissella confusa strain pl9001 inhibited the binding of helicobacter pylori to human gastric-cell line mkn-45 cells by more than 90%. spent culture supernatant of pl9001 rapidly decreased the viability of h. pylori, rupturing cell walls. the results suggest that pl9001 is a probiotic that can reduce the infectivity and persistence of h. pylori. | 2002 | 12200324 |
| a pcr-based method for identification of lactobacilli at the genus level. | we developed a polymerase chain reaction (pcr)-based method for the identification of lactobacilli at the genus level. one specific primer, lblma1-rev, was designed by analysing similarities between the nucleotide sequence of the spacer between the 16s and 23s rrna genes in a number of lactobacillus strains. amplification with lblma1-rev and r16-1, a universal primer, generated a pcr product for 23 lactobacillus species. electrophoresis did not reveal any discrete bands when escherichia coli, la ... | 2002 | 12351242 |
| phylogenetic, amino acid content and indel analyses of the beta subunit of dna-dependent rna polymerase of gram-positive and gram-negative bacteria. | in this study, we have sequenced the rpob gene, encoding the beta subunit of dna-dependent rna polymerase, from a selection of gram-positive and gram-negative bacteria. the presence of insertions and deletions (indels) in the beta subunit separate the gram-positive and gram-negative bacteria from each other and support the division of the gram-positive organisms into two clades based on dna g+c content. phylogenetic and amino acid content analyses further separate the clostridia from bacilli, le ... | 2002 | 12361249 |
| microbiological and fermentation characteristics of togwa, a tanzanian fermented food. | selected microbiological and metabolic characteristics of sorghum, maize, millet and maize-sorghum togwa were investigated during natural fermentation for 24 h. the process was predominated by lactic acid bacteria (lab) and yeasts. the mesophiles, lactic acid bacteria, and yeasts increased and the enterobacteriaceae decreased to undetectable levels within 24 h. the isolated microorganisms were tentatively identified as lactobacillus plantarum, lactobacillus brevis, lactobacillus fermentum, lacto ... | 2003 | 12423921 |
| interactive inhibition of meat spoilage and pathogenic bacteria by lysozyme, nisin and edta in the presence of nitrite and sodium chloride at 24 degrees c. | to develop a nisin- and lysozyme-based antimicrobial treatment for use with processed ham and bologna, in vitro experiments were conducted to determine whether inhibition enhancing interactions occur between the antimicrobials lysozyme, chrisin (a commercial nisin preparation), edta, nacl and nano(2). inhibitory interactions were observed between a number of agents when used against specific pathogenic and spoilage bacteria. the observed interactions included lysozyme with edta (enterococcus fae ... | 2003 | 12423927 |
| the biodiversity of lactic acid bacteria in greek traditional wheat sourdoughs is reflected in both composition and metabolite formation. | lactic acid bacteria (lab) were isolated from greek traditional wheat sourdoughs manufactured without the addition of baker's yeast. application of sodium dodecyl sulfate-polyacrylamide gel electrophoresis of total cell protein, randomly amplified polymorphic dna-pcr, dna-dna hybridization, and 16s ribosomal dna sequence analysis, in combination with physiological traits such as fructose fermentation and mannitol production, allowed us to classify the isolated bacteria into the species lactobaci ... | 2002 | 12450829 |
| isolation, characterisation and identification of lactic acid bacteria from bushera: a ugandan traditional fermented beverage. | one hundred and thirteen strains of lactic acid bacteria (lab) were selected from 351 isolates from 15 samples of traditionally fermented household bushera from uganda and also from laboratory-prepared bushera. isolates were phenotypically characterised by their ability to ferment 49 carbohydrates using api 50 chl kits and additional biochemical tests. coliforms, yeasts and lab were enumerated in bushera. the ph, volatile organic compounds and organic acids were also determined. the lab counts i ... | 2003 | 12494920 |
| specific probiotic characterization of weissella hellenica ds-12 isolated from flounder intestine. | a total of 199 microorganisms were isolated from the intestinal contents of flounder (paralichthys olivaceus) in a fish farm in seoul, korea. among these strains, ds-12 was selected as a candidate for flounder probiotics because of its excellent exhibition of antimicrobial activity against fish pathogens such as edwardsiella, pasteurella, aeromonas, and vibrio, and initiate growth in 10% nacl, 10% bile, and in broth at ph 3 for 90 min. this strain was gram-positive, and catalase-negative coccoid ... | 1998 | 12501410 |
| phylogenetic diversity of lactic acid bacteria associated with paddy rice silage as determined by 16s ribosomal dna analysis. | a total of 161 low-g+c-content gram-positive bacteria isolated from whole-crop paddy rice silage were classified and subjected to phenotypic and genetic analyses. based on morphological and biochemical characters, these presumptive lactic acid bacterium (lab) isolates were divided into 10 groups that included members of the genera enterococcus, lactobacillus, lactococcus, leuconostoc, pediococcus, and weissella: analysis of the 16s ribosomal dna (rdna) was used to confirm the presence of the pre ... | 2003 | 12514026 |
| characterization of sourdough lactic acid bacteria based on genotypic and cell-wall protein analyses. | to evaluate the effectiveness of two independent methods in differentiating a large population of lactic acid bacteria (lab) isolated from wheat flours and sourdoughs and to correlate eventual differences/similarities among strains with their geographical origin and/or process parameters. | 2003 | 12631200 |
| fatal case of endocarditis due to weissella confusa. | this is the first reported case of endocarditis due to the lactobacillus-like vancomycin-resistant gram-positive bacillus weissella confusa. full identification and susceptibility testing of lactobacillus-like organisms recovered in blood culture should be performed for patients with clinical presentations that suggest endocarditis. | 2003 | 12734290 |
| crystallization and preliminary x-ray analysis of weissella viridescens femx udp-murnac-pentapeptide:l-alanine ligase. | synthesis of the cell-wall peptidoglycan of firmicutes involves a unique family of peptide-bond-forming enzymes that use amino-acyl-trnas as substrates and are referred to as fem proteins as they are factors essential for methicillin resistance in staphylococcus aureus. the femx udp-murnac-pentapeptide:l-alanine ligase of weissella viridescens was overexpressed, purified and crystallized. native data were collected to 1.7 a resolution. the crystals belong to space group p2(1), with unit-cell par ... | 2003 | 12777772 |
| identification of lactic acid bacteria isolated from human vaginal secretions. | a total of 57 lactic acid bacteria were isolated from the vaginal secretions of 259 patients. of these strains, 37 were isolated from patients attending pre-natal clinics and the remaining strains from patients attending post-natal clinics. the strains were identified by using simple physiological and biochemical tests and their phenotypic relatedness determined by numerical analysis of total soluble cell protein patterns. the genotypic relatedness of representative strains selected from each of ... | 2003 | 12785305 |
| bacteriophage ecology in commercial sauerkraut fermentations. | knowledge of bacteriophage ecology in vegetable fermentations is essential for developing phage control strategies for consistent and high quality of fermented vegetable products. the ecology of phages infecting lactic acid bacteria (lab) in commercial sauerkraut fermentations was investigated. brine samples were taken from four commercial sauerkraut fermentation tanks over a 60- or 100-day period in 2000 and 2001. a total of 171 phage isolates, including at least 26 distinct phages, were obtain ... | 2003 | 12788716 |
| effect of proteolytic starter cultures as leavening agents of pizza dough. | lactic acid bacteria (lab) and yeasts were selected on the basis of in vitro proteolytic activity against wheat gluten protein and then assayed as leavening agents for pizza dough. trials were carried out to compare a proteolytic starter (prt(+)), consisting of lactobacillus sakei t56, weissella paramesenteroides a51 and candida krusei g271, and a non-proteolytic starter (prt(-)), consisting of lb. sakei t58, w. paramesenteroides a58 and saccharomyces cerevisiae t22. the proteolytic activity of ... | 2003 | 12810294 |
| thermal resistances and lactate and diacetate sensitivities of bacteria causing bologna discolouration. | this report describes the effects of heat, sodium lactate, and sodium diacetate on the viabilities of weissella viridescens atcc 12706, aerococcus viridans mpl-1 and mpl-b, and carnobacterium viridans atcc baa 336. the latter three organisms were isolated from commercial product and have previously been shown to produce green discolourations in cooked cured bologna. w. viridescens was heat resistant in beef bologna (d(60 degrees c)=14.7 min) but not in apt broth. a. viridans and c. viridans were ... | 2003 | 12915033 |
| novel leuconostoc citreum starter culture system for the fermentation of kimchi, a fermented cabbage product. | to determine the dominant microorganisms involved in kimchi fermentation and to examine their effect on kimchi fermentation, we randomly isolated and characterized 120 lactic acid bacteria from kimchi during a 5-day fermentation at 15 degrees c. leuconostoc citreum was dominant during the early and mid-phases of kimchi fermentation whereas lactobacillus sake/lactobacillus curvatus or lactobacillus brevis were found during later stages. eighty-two out of 120 isolates (68%) were identified as leuc ... | 2003 | 14574101 |
| weissella confusa infection in primate (cercopithecus mona). | | 2003 | 14626220 |
| crystal structures of weissella viridescens femx and its complex with udp-murnac-pentapeptide: insights into femabx family substrates recognition. | members of the femabx protein family are novel therapeutic targets, as they are involved in the synthesis of the bacterial cell wall. they catalyze the addition of amino acid(s) on the peptidoglycan precursor using aminoacylated trna as a substrate. we report here the high-resolution structure of weissella viridescens l-alanine transferase femx and its complex with the udp-murnac-pentapeptide. this is the first structure example of a femabx family member that does not possess a coiled-coil domai ... | 2004 | 14962386 |
| genetic evidence that weissella kimchii choi et al. 2002 is a later heterotypic synonym of weissella cibaria björkroth et al. 2002. | the taxonomic positions of weissella kimchii and weissella cibaria were examined using phenotypic tests, 16s rrna gene sequencing and dna-dna hybridization. the two species displayed very similar sugar-fermentation patterns and produced the d-form of lactic acid. phylogenetic analysis showed that w. cibaria is the closest neighbour of w. kimchii, sharing about 99.7 % similarity in the 16s rrna gene sequence. dna-dna hybridization experiments further reinforced these results, demonstrating a 98.2 ... | 2004 | 15023961 |
| detection of leuconostoc strains at a meat processing plant using polymerase chain reaction. | to simplify the labor-intensive conventional routine testing of samples to detect leuconostoc at a meat processing plant, we developed polymerase chain reaction (pcr) primers specific for leuconostoc from 16s rrna gene sequences. these primers did not detect other common lactic acid bacteria such as lactobacillus plantarum, lact. sake, lact. fermentum, lact. acidophilus and weissella viridescens. pcr with this primer detected all leuconostoc species tested (leu. mesenteroides subsp. mesenteroide ... | 2004 | 15168557 |
| cell envelope analysis of insensitive, susceptible or resistant strains of leuconostoc and weissella genus to leuconostoc mesenteroides fr 52 bacteriocins. | mesenterocins 52a and 52b belong to class ii of lactic acid bacteria bacteriocins. to study susceptibility, insensitivity and resistance to these mesenterocins, four wild-type bacterial strains and four resistant strains, all from leuconostoc or weissella genus, were compared. several cell envelope features were investigated: susceptibilities to antibiotics and to lysozyme, cell morphology and membrane phospholipids contents. the strain insensitive to the two mesenterocins appeared to be resista ... | 2004 | 15556709 |
| characterization and identification of lactic acid bacteria in "morcilla de burgos". | a total of 176 lactic acid bacteria (lab) isolated from a typical spanish blood sausage called "morcilla de burgos" were identified by means of phenotypic characteristics and 16s rdna rflp (ribotyping). lab were isolated from "morcilla" of different producers and in different storage periods, which includes unpackaged, vacuum and modified atmosphere packaged "morcilla" and vacuum packed and pasteurised "morcilla". the knowledge of specific spoilage bacteria of "morcilla de burgos" will be useful ... | 2005 | 15582739 |
| physical and genetic map of the weissella paramesenteroides dsmz 20288t chromosome and characterization of different rrn operons by its analysis. | the weissella paramesenteroides dsmz 20288t chromosome was analysed by pulsed-field gel electrophoresis, enabling the construction of a physical and genetic map. a total of 21 recognition sites of the restriction enzymes asci, i-ceui, noti and sfii were mapped on the chromosome, which was found to be circular with an estimated size of 2026 kb. this is believed to constitute the first study into the genomic organization of a strain of this genus, addressing the localization of important chromosom ... | 2004 | 15583160 |
| enhancement in ex vivo phagocytic capacity of peritoneal leukocytes in mice by oral delivery of various lactic-acid-producing bacteria. | lactic-acid-producing bacteria (labs) are known to have immunomodulating activity. in the current study, various labs were tested for their immunity-enhancing activity, especially the phagocytic activity of leukocytes. viable but not heat-killed cells of weissella kimchii strain pl9001, lactobacillus fermentum strain pl9005, and l. plantarum strain pl9011 significantly increased the ex vivo phagocytic capacity of mouse peritoneal leukocytes to ingest fluorescein isothiocyanate (fitc)-labeled esc ... | 2004 | 15696260 |
| isolation and identification of lactic acid bacteria from soil using an enrichment procedure. | to survey, and identify and classify the ecological distribution of lactic acid bacteria from soil in japan and taiwan. | 2005 | 15715644 |
| identification to the species level of lactobacillus isolated in probiotic prospecting studies of human, animal or food origin by 16s-23s rrna restriction profiling. | the accurate identification of lactobacillus and other co-isolated bacteria during microbial ecological studies of ecosystems such as the human or animal intestinal tracts and food products is a hard task by phenotypic methods requiring additional tests such as protein and/or lipids profiling. | 2005 | 15788104 |
| developing microbial spoilage population in vacuum-packaged charcoal-broiled european river lamprey (lampetra fluviatilis). | microbiological and sensory changes in vacuum-packaged charcoal-broiled river lampreys from three lamprey processing plants were monitored as a function of time at 8 degrees c. the lampreys were examined every 7 days up to 8 weeks for aerobic plate count (apc) and lactic acid bacteria (lab). the highest mean apc and lab were 6.01 log cfu/g and 4.86 log cfu/g, respectively. only 6 out of 15 lots reached an apc value of 7.0 log cfu/g during storage. the sensory scores remained at the baseline leve ... | 2004 | 15862876 |
| structure-based site-directed mutagenesis of the udp-murnac-pentapeptide-binding cavity of the femx alanyl transferase from weissella viridescens. | weissella viridescens femx (femx(wv)) belongs to the fem family of nonribosomal peptidyl transferases that use aminoacyl-trna as the amino acid donor to synthesize the peptide cross-bridge found in the peptidoglycan of many species of pathogenic gram-positive bacteria. we have recently solved the crystal structure of femx(wv) in complex with the peptidoglycan precursor udp-murnac-pentapeptide and report here the site-directed mutagenesis of nine residues located in the binding cavity for this su ... | 2005 | 15901708 |
| analysis of kimchi microflora using denaturing gradient gel electrophoresis. | a polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge) technique was used to determine the microfloral composition during the fermentation of kimchi, a traditional korean fermented vegetable food. the kimchi was fermented at 10 degrees c or 20 degrees c for 30 or 20 days, respectively. dgge of the partially amplified 16s rdna was performed and the most intense bands sequenced. the application of this culture-independent molecular technique determined that the lactic acid ... | 2005 | 15992614 |
| potential application of ribosomal intergenic spacer analysis to the microbial community analysis of agronomic products. | ribosomal intergenic spacer analysis (risa) has been applied to the microbial community analysis of agronomic products in combination with a simple and rapid dna extraction method, consisting of a one-step extraction and two-step purification, for a variety of agronomic products. risa appears to be a useful tool for the study of the community structures of food-associated microbes and their use as a unique fingerprinting signature for each agronomic product. sequencing analyses of amplicons gene ... | 2005 | 15998122 |
| citi, a transcription factor involved in regulation of citrate metabolism in lactic acid bacteria. | a large variety of lactic acid bacteria (lab) can utilize citrate under fermentative conditions. although much information concerning the metabolic pathways leading to citrate utilization by lab has been gathered, the mechanisms regulating these pathways are obscure. in weissella paramesenteroides (formerly called leuconostoc paramesenteroides), transcription of the citmdefcgrp citrate operon and the upstream divergent gene citi is induced by the presence of citrate in the medium. although genet ... | 2005 | 16030208 |
| identification of enterococcal, streptococcal and weissella species in the faecal flora of individually owned dogs. | to improve the limited information on the composition of the faecal gram-positive coccal flora of healthy dogs by the use of a molecular identification method. | 2005 | 16033466 |
| characterization of weissella kimchii pl9023 as a potential probiotic for women. | from more than 100 lactic acid bacteria, weissella kimchii pl9023 was selected as producing the most hydrogen peroxide and was characterized as a probiotic for women. w. kimchii pl9023 inhibited the growth and adherence of vaginal isolates of candida albicans, escherichia coli, staphylococcus aureus and streptococcus agalactiae. the surface components involved in adherence of w. kimchii pl9023 to vaginal epithelial cells appeared to be glycoproteins, as determined by susceptibility to proteinase ... | 2005 | 16055277 |
| bacterial community structure in kimchi, a korean fermented vegetable food, as revealed by 16s rrna gene analysis. | kimchi is a traditional korean food fermented from a variety of vegetables. we elucidated the microbial community structure of five commercially produced kimchis made from chinese cabbage by examining culture-independent 16s rrna gene clone libraries. most of the clones (347 out of 348) belonged to lactic acid bacteria and included several species of the genera lactobacillus, leuconostoc and weissella. weissella koreensis was found in all the samples and predominated in three of them (42.6-82%). ... | 2005 | 16084269 |
| diversity and technological properties of predominant lactic acid bacteria from fermented cassava used for the preparation of gari, a traditional african food. | traditional fermentation of cassava is dominated by a lactic acid bacteria (lab) population. fermentation is important for improving product flavour and aroma as well as safety, especially by reduction of its toxic cyanogenic glucosides. the production of gari from cassava in benin typically occurs on a household or small industrial scale, and consequently suffers from inconsistent product quality and may not always be safe for consumption. therefore, the diversity of lab from a typical cassava ... | 2005 | 16104351 |
| monitoring the lactic acid bacterial diversity during shochu fermentation by pcr-denaturing gradient gel electrophoresis. | the presence of lactic acid bacteria (lab) during shochu fermentation was monitored by pcr-denaturing gradient gel electrophoresis (dgge) and by bacteriological culturing. no lab were detected from fermented mashes by pcr-dgge using a universal bacterial pcr primer set. however, pcr-dgge using a new primer specific for the 16s rdna of lactococcus, streptococcus, tetragenococcus, enterococcus, and vagococcus and two primers specific for the 16s rdna of lactobacillus, pediococcus, leuconostoc, and ... | 2005 | 16233780 |
| phenotypic identification and technological properties of lactic acid bacteria isolated from traditionally processed fish products of the eastern himalayas. | sukako maacha, gnuchi, sidra and sukuti are traditional smoked and sun-dried fish products of the eastern himalayan regions of nepal and india. a total of 40 samples of sukako maacha (14), gnuchi (6), sidra (10) and sukuti (10) were collected and were analysed for microbial load. population of lactic acid bacteria (lab) as well as aerobic mesophilic counts ranged from 4.7-8.3 to 5.1-8.5 log cfu g(-1), respectively. a total of 189 strains of lab were isolated from sukako maacha, gnuchi, sidra and ... | 2006 | 16278028 |
| coaggregation ability of weissella cibaria isolates with fusobacterium nucleatum and their adhesiveness to epithelial cells. | in the present study, we evaluated the ability of weissella cibaria isolated from the oral cavity to coaggregate with fusobacterium nucleatum, and the adhesiveness of these strains to epithelial cells. w. cibaria efficiently coaggregated with f. nucleatum, and adhered to epithelial cells. we tested the effects of various factors on the coaggregation. the coaggregation and adhesiveness of w. cibaria disappeared upon exposure to pronase or licl, suggesting that proteinaceous components on the surf ... | 2005 | 16288835 |
| inhibitory effect of weissella cibaria isolates on the production of volatile sulphur compounds. | the objective of this study was to characterize the inhibitory effects of weissella cibaria isolates on volatile sulphur compounds (vsc) production both in vitro and in vivo. | 2006 | 16489950 |
| diversity of weissella viridescens strains associated with "morcilla de burgos". | unidentified heterofermentative lactic acid bacterium (lab) isolates detected earlier in vacuum or modified atmosphere (ma) packaged "morcilla de burgos", a spanish cooked meat product, were identified in the present study. in the previous study, these strains had been studied by numerical analysis of hindiii rflp- and phenotyping. however, neither of these methods had resulted in species identification. even these unknown strains clustered together in the numerical analysis of the rflp patterns ... | 2006 | 16503360 |
| usefulness of length heterogeneity-pcr for monitoring lactic acid bacteria succession during maize ensiling. | the use of length-heterogeneity pcr was explored to monitor lactic acid bacteria succession during ensiling of maize. bacterial diversity was studied during the fermentation of 30-day-old maize in optimal and spoilage-simulating conditions. a length heterogeneity pcr profile database of lactic acid bacteria isolated from the silage and identified by 16s rrna gene sequencing was established. although interoperonic 16s rrna gene length polymorphisms were detected in some isolates, strain analysis ... | 2006 | 16542413 |
| lactic acid bacteria associated with wine grapes from several australian vineyards. | the detection and isolation of lactic acid bacteria by enrichment methods from wine grapes cultivated in vineyards located in new south wales, australia. | 2006 | 16553726 |
| microbial population dynamics of kimchi, a fermented cabbage product. | lactic acid bacteria are known to perform significant roles in the fermentation of kimchi, a fermented cabbage product. however, the microbial population dynamics inherent to kimchi fermentation remain to be clearly elucidated. in this study, we have characterized the microbial dynamics via the identification of a total of 970 bacterial isolates, representing 15 species of the genera lactobacillus, leuconostoc, and weissella, all of which were primarily identified by pcr-based restriction enzyme ... | 2006 | 16553862 |
| lactic acid bacteria isolated from canine faeces. | lactic acid bacteria (lab) were isolated and sequenced from the faeces of healthy dogs. five of these strains were selected and further characterized to clarify the potential of these strains as probiotics for canine. | 2006 | 16834600 |
| defined multi-species semi-liquid ready-to-use sourdough starter. | this paper aimed at setting up a protocol for the manufacture of a defined multi-species semi-liquid ready-to-use sourdough starter consisting of lactic acid bacteria (lab) selected on the basis of their capacity of rapid acidification and adaptation to several technological factors. preliminarily, 56 strains of sourdough lab were screened based on the acidification kinetics during sourdough fermentation. the influence of temperature (25-37 degrees c), nacl (2%, w/w, of wheat flour), initial cel ... | 2007 | 16943090 |
| effect of weissella cibaria isolates on the formation of streptococcus mutans biofilm. | the objective of this study was to isolate and identify lactic acid bacteria able to inhibit the in vitro formation of streptococcus mutans biofilm as well as the in vivo formation of oral biofilm. two strains, cms1 and cms3, exhibiting profound inhibitory effects on the formation of s. mutans biofilm and the proliferation of s. mutans, were isolated from children's saliva and identified as weissella cibaria by 16s rdna sequencing. the water-soluble polymers produced from sucrose by the w. cibar ... | 2006 | 16946611 |
| leuconostoc carnosum associated with spoilage of refrigerated whole cooked hams in greece. | a polyphasic taxonomic approach was used to identify a major atypical group of gas-forming, arginine-negative lactic acid bacteria associated with spoilage of whole (nonsliced) refrigerated (4 degrees c) cooked hams produced in two greek industrial meat plants. biochemical characterization revealed that the ham isolates shared their phenotypic properties with leuconostoc carnosum, weissella viridescens, and weissella hellenica. however, gas chromatographic analysis of cellular fatty acids clearl ... | 2006 | 16995536 |
| alteration of the canine small-intestinal lactic acid bacterium microbiota by feeding of potential probiotics. | five potentially probiotic canine fecal lactic acid bacterium (lab) strains, lactobacillus fermentum lab8, lactobacillus salivarius lab9, weissella confusa lab10, lactobacillus rhamnosus lab11, and lactobacillus mucosae lab12, were fed to five permanently fistulated beagles for 7 days. the survival of the strains and their potential effects on the indigenous intestinal lab microbiota were monitored for 17 days. denaturing gradient gel electrophoresis (dgge) demonstrated that the five fed lab str ... | 2006 | 17021203 |
| antimicrobial susceptibility of non-enterococcal intrinsic glycopeptide-resistant gram-positive organisms. | non-enterococcal gram-positive bacteria that are intrinsically vancomycin-resistant have been infrequently isolated in association with serious infections. however, well-documented infections have lately been reported with increasing frequency. because these organisms may be pathogens, we tested the mics of 19 antimicrobial agents by the agar dilution method for predicting susceptibility. the activity of these antimicrobial agents was assessed against 28 strains (lactobacillus rhamnosus, 6; lact ... | 2007 | 17049799 |
| severe infective endocarditis of native valves caused by weissella confusa detected incidentally on echocardiography. | weissella confusa is gram-positive, catalase-negative, short rod or coccobacillus that has been isolated from a wide variety of habitats. we describe a rare, severe infective endocarditis of native valves caused by w. confusa that was detected incidentally on echocardiography. the patient recovered completely with valvular replacement plus antibiotic therapy. | 2007 | 17052757 |
| microbial community structure and dynamics of dark fire-cured tobacco fermentation. | the italian toscano cigar production includes a fermentation step that starts when dark fire-cured tobacco leaves are moistened and mixed with ca. 20% prefermented tobacco to form a 500-kg bulk. the dynamics of the process, lasting ca. 18 days, has never been investigated in detail, and limited information is available on microbiota involved. here we show that toscano fermentation is invariably associated with the following: (i) an increase in temperature, ph, and total microbial population; (ii ... | 2007 | 17142368 |
| enzymatic activities of lactic acid bacteria isolated from cornetto di matera sourdoughs. | forty-one strains of lactic acid bacteria (lab) isolated from cornetto di matera sourdoughs were screened for their enzymatic activities, to elucidate their possible roles during the fermentation process. urease, peptidase, phytase, phosphatase and beta-glucosidase activities were measured spectrophotometrically using synthetic substrates. proteolytic activities were examined in model doughs, using neutral and acidified sterile doughs as controls. all strains had low urease, glutamyl aminopeptid ... | 2007 | 17174429 |
| glucan and fructan production by sourdough weissella cibaria and lactobacillus plantarum. | after a large screening on sourdough lactic acid bacteria, exopolysaccharide (eps)-forming strains of weissella cibaria, lactobacillus plantarum, and pediococcus pentosaceus were selected. after 6 days of incubation at 30 degrees c, the synthesis of eps in mrs-based broth ranged from 5.54 to 7.88 mg ml-1. eps had an apparent molecular mass of ca. 104 da. as shown by carbohydrate consumption, the synthesis of eps was found from sucrose only. two types of homopolysaccharides were synthesized: gluc ... | 2006 | 17177514 |
| characterisation and biochemical properties of predominant lactic acid bacteria from fermenting cassava for selection as starter cultures. | a total of 375 lactic acid bacteria were isolated from fermenting cassava in south africa, benin, kenya and germany, and were characterised by phenotypic and genotypic tests. these could be divided into five main groups comprising strains of facultatively heterofermentative rods, obligately heterofermentative rods, heterofermentative cocci, homofermentative cocci and obligately homofermentative rods, in decreasing order of predominance. most of the facultatively heterofermentative rods were iden ... | 2007 | 17188771 |
| congruence of evolutionary relationships inside the leuconostoc-oenococcus-weissella clade assessed by phylogenetic analysis of the 16s rrna gene, dnaa, gyrb, rpoc and dnak. | the phylogenetic structure of the leuconostoc-oenococcus-weissella clade was evaluated by comparison of 16s rrna gene, dnaa, gyrb, rpoc and dnak sequence analysis. phylogenies obtained with the different genes were in overall good agreement and a well-supported, almost fully resolved phylogenetic tree was obtained when the combined data were analysed in a bayesian approach. a rapid basal diversification of the three genera is suggested. evolutionary rates of the 16s rrna gene in these genera see ... | 2007 | 17267964 |
| dynamics and biodiversity of populations of lactic acid bacteria and acetic acid bacteria involved in spontaneous heap fermentation of cocoa beans in ghana. | the ghanaian cocoa bean heap fermentation process was studied through a multiphasic approach, encompassing both microbiological and metabolite target analyses. a culture-dependent (plating and incubation, followed by repetitive-sequence-based pcr analyses of picked-up colonies) and culture-independent (denaturing gradient gel electrophoresis [dgge] of 16s rrna gene amplicons, pcr-dgge) approach revealed a limited biodiversity and targeted population dynamics of both lactic acid bacteria (lab) an ... | 2007 | 17277227 |
| weissellicin 110, a newly discovered bacteriocin from weissella cibaria 110, isolated from plaa-som, a fermented fish product from thailand. | weissella cibaria 110, isolated from the thai fermented fish product plaa-som, was found to produce a bacteriocin active against some gram-positive bacteria. bacteriocin activity was not eliminated by exposure to high temperatures or catalase but was destroyed by exposure to the proteolytic enzymes proteinase k and trypsin. the bacteriocin from w. cibaria 110 was purified, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that the purified bacteriocin contained one protein ban ... | 2007 | 17293526 |
| identification of lactic acid bacteria isolated from human blood cultures. | fifteen lactic acid bacterial strains were isolated from blood cultures from 15 different patients in the faculty hospital in brno, czech republic. all strains were identified using biochemical tests and repetitive pcr using the (gtg)5 primer. doubtful identification results were confirmed by whole-cell protein analysis. the strains were assigned to the genera lactobacillus (eight strains representing seven species), leuconostoc (six strains representing four species) and weissella (one strain). ... | 2007 | 17328753 |
| species diversity and relative abundance of vaginal lactic acid bacteria from women in uganda and korea. | lactobacilli play an important role in maintaining vaginal health of women. the aim of this study was to compare the species richness and relative abundance of lactobacillus and other lactic acid bacteria in women of two geographically distant countries, uganda and korea. | 2007 | 17381754 |
| quantitative analysis of the intestinal bacterial community in one- to three-week-old commercially reared broiler chickens fed conventional or antibiotic-free vegetable-based diets. | to explore the effect of drug-free poultry production on the intestinal microflora of broiler chickens, the bacterial community of this environment was quantitatively profiled in both conventionally reared birds and birds reared without antibiotic growth promotants (agps) on a vegetable-based diet. | 2007 | 17381758 |
| pcr monitoring of lactobacillus and bifidobacterium dynamics in fermentations by piglet intestinal microbiota. | a new group-specific primer (lact71r), targeting the 16s-23s rdna intergenic spacer region of lactobacillus, was tested in its specificity to amplify rdna of lactobacilli from piglet intestinal origin by polymerase chain reaction (pcr). lact71r and lab0677f, a lactobacillus group-specific primer targeting the 16s rdna, generated a common amplicon by pcr with dna from lactobacillus and pediococcus reference strains, but not from weissella strains. sequence analysis of clones obtained by pcr ampli ... | 2007 | 17440917 |
| isolation and characterization of lactic acid bacteria from lakes. | lactic acid bacteria (lab) were isolated from lake-water samples collected at 7 lakes distributed in yamanashi prefecture, japan. sampling was performed year round. 112 cultures were isolated and divided into classes by phenotype and then into groups by restriction fragment length polymorphism analysis and sequencing of 16s ribosomal dna. phenotypic and biochemical characteristics identified eleven different bacterial groups (a to k), and the results showed that the isolates represented seven ge ... | 2007 | 17440921 |