| butyrivibrio hungatei sp. nov. and pseudobutyrivibrio xylanivorans sp. nov., butyrate-producing bacteria from the rumen. | two novel gram-negative, anaerobic, non-spore-forming, butyrate-producing bacterial species, strains mz 5t and jk 615t, were isolated from the rumen fluid of cow and sheep. both strains were curved rods that were motile by means of single polar or subpolar flagellum and common in the rumen microbial ecosystem. strain mz 5t produced high xylanase, proteinase, pectin hydrolase and dnase activities; 1,4-beta-endoglucanase was also detected in the culture medium. the bacterium utilized a wide range ... | 2003 | 12656174 |
| detection of possible ai-2-mediated quorum sensing system in commensal intestinal bacteria. | the vibrio harveyi strain bb170-autoinducer bioassay was used to detect possible quorum sensing autoinducer-2 molecule (ai-2) in culture fluids of commensal intestinal bacteria. culture fluids of bacteroides vulgatus, clostridium proteoclasticum, escherichia coli, eubacterium rectale, lachnospira multipara, pseudobutyrivibrio ruminis, roseburia intestinalis, ruminococcus albus and ruminococcus flavefaciens contained ai-2-like molecules. the pcr bands from some of the tested strains could be also ... | 2008 | 18661296 |
| sucrose phosphorylase of the rumen bacterium pseudobutyrivibrio ruminis strain a. | to verify the taxonomic affiliation of bacterium butyrivibrio fibrisolvens strain a from our collection and to characterize its enzyme(s) responsible for digestion of sucrose. | 2009 | 19320946 |
| phylogenetic analysis of 16s rrna gene sequences reveals rumen bacterial diversity in yaks (bos grunniens). | six matured male yaks (bos grunniens) with a mean live weight of 450 +/- 23 kg (mean +/- sd), were housed indoors in metabolism cages and fed pelleted lucerne (medicago sativum). after an adjustment period of 24 days of feeding the diet, samples of rumen content were obtained for analysis of the bacteria in the liquor. the diversity of rumen bacteria was investigated by constructing a 16s rrna gene clone library using the general bacterial primers f27 and r1492. a total of 130 clones, comprising ... | 2010 | 19757178 |
| detection and identification of rumen bacteria constituting a fibrolytic consortium dominated by fibrobacter succinogenes. | a fibrolytic consortium, dominated by the rumen cellulolytic bacterium fibrobacter succinogenes, was artificially constructed on hay stems to detect and identify rumen bacteria that can potentially interact with f. succinogenes. consortium-bacterial members were determined by dgge and sequencing analysis targeted bacterial 16s rdna. an artificial consortium was formed in a 2-step incubation of hay stems; the first step with group 1, 2 or 3 f. succinogenes strains, the second step with rumen flui ... | 2010 | 20163675 |
| dominant bacterial communities in the rumen of gayals (bos frontalis), yaks (bos grunniens) and yunnan yellow cattle (bos taurs) revealed by denaturing gradient gel electrophoresis. | the dominant rumen bacteria in gayals, yaks and yunnan yellow cattle were investigated using pcr-dgge approach. the analysis of dgge profiles, identification of dominant bands and phylogenetic analysis 16s rdna sequences in dgge profiles were combined to reveal the dominant bacterial communities and compared the differences between those cattle species. dgge profiles revealed that gayals had the most abundant dominant bacteria and the lowest similarity of intraspecies between individuals than ot ... | 2010 | 21140219 |
| molecular diversity of bacteria in yunnan yellow cattle (bos taurs) from nujiang region, china. | the rumen content of four yunnan yellow cattle (bos taurs) were collected to determine the bacteria diversity by using 16s rrna gene sequence analysis. a total of 129 sequences were examined and the sequences were referred as 107 otu (operational taxonomy unit) according to the similarity level of 97% in gene sequence. similarity analysis revealed that yunnan yellow cattle had 12 sequences (10 otu) shared 97% or greater similarity with cultured rumen bacteria butyrivibrio fibrisolvens, succinicl ... | 2011 | 21598111 |
| fructanolytic and saccharolytic enzymes of the rumen bacterium pseudobutyrivibrio ruminis strain 3--preliminary study. | p. ruminis strain 3 was isolated from the ovine rumen and identified on the basis of comparison of its 16s rrna gene with genbank. the bacterium was able to grow on timothy grass fructan, inulin, sucrose, fructose and glucose as a sole carbon source, reaching absorbance of population in a range of 0.4-1.2. during 1 d the bacteria exhausted 92-97% of initial dose of saccharides except for inulin (its utilization did not exceed 33%). the bacterial cell extract catalyzed the degradation of timothy ... | 2010 | 20680564 |
| bacterial diversity in the rumen of gayals (bos frontalis), swamp buffaloes (bubalus bubalis) and holstein cow as revealed by cloned 16s rrna gene sequences. | libraries of rumen bacterial 16s rrna gene sequences of gayals (bos frontalis) and swamp buffaloes (bubalus bubalis) were cloned and sequenced in the present work to compare the bacterial diversity with the third published library of holstein cow. sequence similarity of 97% was used as the definition of operational taxonomic unit (otu). the majority of the 470 sequences retrieved fell into the phyla of low g + c subdivision (329 sequences) and cytophaga-flexibacter-bacteroides (cfb, 123 sequence ... | 2010 | 19662514 |
| isolation and characterization of putative pseudobutyrivibrio ruminis promoters. | novel plasmids were constructed for the analysis of dna fragments from the rumen bacterium pseudobutyrivibrio ruminis. five previously unidentified promoters were characterized using a novel primer extension method to identify transcription start sites. the genes downstream of these promoters were not identified, and their activity in expression of genomic traits in wild-type p. ruminis remains putative. comparison with promoters from this and closely related species revealed a consensus sequenc ... | 2007 | 17768250 |
| effect of fatty acids on growth of conjugated-linoleic-acids-producing bacteria in rumen. | microorganisms with high activity of linoleic acid delta12-cis,delta11-trans-isomerase were isolated from the digestive tract of ruminants and characterized. the isolate with the highest isomerase activity was identified as pseudobutyrivibrio ruminis. the susceptibility of this strain to 3 fatty acids added to the grow medium was determined. a significant inhibition of bacterial growth (during a 3-d period) by linoleic acid (0.1 %) and oleic acid (5 ppm) was observed; no inhibition was found in ... | 2006 | 17007428 |
| β-fructofuranosidase and sucrose phosphorylase of rumen bacterium pseudobutyrivibrio ruminis strain 3. | the subject of this study was the fructan and sucrose degrading enzymes of bacterium pseudobutyrivibrio ruminis strain 3. it was stated that cell extract from bacteria growing on inulin contained β-fructofuranosidase (ec 3.2.1.80 and/or ec 3.2.1.26) and sucrose phosphorylase (ec 2.4.1.7), while the bacteria maintained on sucrose showed only phosphorylase. partially purified β-fructofuranosidase digested inulooligosaccharides and sucrose to fructose or fructose and glucose, respectively, but was ... | 2012 | 22805847 |
| isolation of pseudobutyrivibrio ruminis and pseudobutyrivibrio xylanivorans from rumen of creole goats fed native forage diet. | we isolated and identified functional groups of bacteria in the rumen of creole goats involved in ruminal fermentation of native forage shrubs. the functional bacterial groups were evaluated by comparing the total viable, total anaerobic, cellulolytic, hemicellulolytic, and amylolytic bacterial counts in the samples taken from fistulated goats fed native forage diet (atriplex lampa and prosopis flexuosa). alfalfa hay and corn were used as control diet. the roll tubes method increased the possibi ... | 2012 | 23275250 |
| phosphorolytic cleavage of sucrose by sucrose-grown ruminal bacterium pseudobutyrivibrio ruminis strain k3. | rumen bacterium pseudobutyrivibrio ruminis strain k3 utilized over 90% sucrose added to the growth medium as a sole carbon source. zymographic studies of the bacterial cell extract revealed the presence of a single enzyme involved in sucrose digestion. thin layer chromatography showed fructose and glucose-1-phosphate (glc1p) as end products of the digestion of sucrose by identified enzyme. the activity of the enzyme depended on the presence of inorganic phosphate and was the highest at the conce ... | 2010 | 20680577 |