| twelve aberrant strains of staphylococcus aureus subsp. aureus from clinical specimens. | a new biovar of staphylococcus aureus subsp. aureus was isolated from human clinical specimens and described on the basis of studies of 12 isolates that were compared with 11 standard reference strains. both dna hybridization experiments and numerical taxonomy analysis demonstrated that these strains were strictly related to s. aureus subsp. aureus; however, they were significantly different from the latter. the atypical strains belonging to the new biovar can be distinguished from typical s. au ... | 1993 | 8370737 |
| simple enzymatic method for rapid identification of a staphylococcus aureus subspecies aureus biovar. | in order to correctly identify a new biovar of staphylococcus aureus subsp, aureus, (nbsa) a simple, rapid, and reliable enzymatic assay was developed. the assay was based on the detection of the production of three enzymes: alpha-glucosidase, beta-glucosidase and beta-n-acetyl-glucosaminidase. of a total of 46 isolates of staphylococcus aureus subsp. aureus from clinical specimens, the new assay correctly identified 19 as nbsa and 27 as typical staphylococcus aureus subsp. aureus. among the 19 ... | 1997 | 9352265 |
| antibacterial effects of minerals from ores indigenous to korea. | we tested the antibacterial properties of a mix of minerals consisting mainly of sericite, talc, and halloysite from korea. the preparation showed clear growth inhibition of the gram-negative bacteria escherichia coli and pseudomonas aeruginosa, and the gram-positive bacteria staphylococcus aureus subsp. aureus, s. epidermidis, and bacillus cereus, as well as the anaerobic bacterium propionibacterium acnes. these results indicate that this preparation, made from ore minerals indigenous to korea, ... | 2009 | 20112878 |
| complete genome sequence of staphylococcus aureus strain jkd6008, an st239 clone of methicillin-resistant staphylococcus aureus with intermediate-level vancomycin resistance. | we report here the complete 2.92-mb genome sequence of a clinical isolate of methicillin-resistant staphylococcus aureus subsp. aureus that demonstrates intermediate-level vancomycin resistance. the strain, named jkd6008, belongs to multilocus sequence type 239 and was isolated from the bloodstream of a patient in new zealand in 2003. | 2010 | 20802046 |
| [antimicrobial activities of norfloxacin against clinical isolates from ocular infections]. | in order to evaluate antimicrobial activity of norfloxacin (nflx), minimum inhibitory concentration (mics) of nflx and control drugs were determined against clinical isolates from ocular infections that were obtained in our laboratory from july, 1993 to december, 1994. the results are summarized as follows; 1. compared to mic distributions of nflx against clinical isolates from ocular infections studied in 1986 and 1987, the mic80 of nflx against corynebacterium spp., enterobacter spp., serratia ... | 1995 | 7474324 |
| genomic variability of staphylococcus aureus and the other coagulase-positive staphylococcus species estimated by macrorestriction analysis using pulsed-field gel electrophoresis. | the genomic dnas of 95 culture collection and hospital staphylococcus aureus subsp. aureus strains of various origins, as well as the genomic dnas of other coagulase-positive staphylococcus species, were cleaved with restriction endonuclease smai and subjected to pulsed-field gel electrophoresis. the levels of similarity of the smai restriction patterns of the s. aureus subsp. aureus strains varied from 30 to 100%, which is considered characteristic of this species; thus, these organisms belonge ... | 1996 | 8573498 |
| [antimicrobial activities of cefteram against recently clinically detected and isolated strains from patients with dental infections]. | to investigate the antibiotic activity of cefteram (cftm), the minimum inhibitory concentrations (mics) of cftm and of the control drugs were determined against clinically isolated strains received from november 1991 to april 1993 from 19 dental facilities throughout the country, as well as against clinically isolated strains from samples obtained at this center from patients with dental infectious diseases, and the following results were obtained. 1. 430 strains were detected in 198 cases but i ... | 1994 | 8151907 |
| [a case of sepsis caused by methicillin-resistant staphylococcus aureus aureus (mrsa) following retropubic prostatectomy]. | although severe infection caused by methicillin-resistant staphylococcus aureus (mrsa) has become a serious social problem in medical facilities, it is relatively uncommon in the urological field. it is supposed that mrsa in the urine is asymptomatic and mrsa urinary colonization is not likely to occur compared with the other staphylococci. we report a case of sepsis caused by mrsa that occurred in the patient following retropubic prostatectomy. he had had a urinary catheter indwelt and had rece ... | 1994 | 7832074 |
| [antimicrobial activities of cefditoren against clinical isolates obtained from outpatients]. | to examine the antimicrobial activity of cefditoren (cdtr) against strains clinically isolated from outpatients at this hospital from november, 1993 to february, 1994, the minimum inhibitory concentrations (mics) were determined including those of the control drugs. the results were as follows: 1. cdtr showed strong antimicrobial activities against staphylococcus aureus subsp. aureus, streptococcus pyogenes and streptococcus pneumoniae. the mics of cdtr against benzylpenicillin-insensitive or -r ... | 1994 | 7807697 |
| [antimicrobial activities of sulbactam/ampicillin against clinically isolated microbial strains]. | antimicrobial activities were examined for sulbactam/ampicillin (sbt/abpc) against clinically isolated microbial strains in 1987, 1990, 1994. besides, the beta-lactamase productivity and mics of these strains were measured, and the following conclusions were obtained. 1. the ratio of beta-lactamase producing strains were 90% of methicillin (dmppc)-susceptible staphylococcus aureus subsp. aureus (mssa), about 80% of dmppc-resistant s. aureus (mrsa), 100% of escherichia coli, klebsiella pneumoniae ... | 1995 | 7783316 |
| [antibacterial activities of fosfomycin against recent clinical isolates from patients of otitis media and otitis externa]. | clinical isolates from patients with otitis media and/or otitis externa were collected at otorhinology clinics nationwide and sent to us during 1989, 1991 and 1993. minimum inhibitory concentrations (mics) of fosfomycin (fom) and of reference drugs against these strains were determined to investigate year-to-year antibacterial activity of fom. a comparative analysis of the results revealed trends described below. 1. the mic90 of fom against staphylococcus aureus subsp. aureus increased 4 times b ... | 1995 | 7745818 |
| [Catalase-negative Staphylococcus aureus subsp. aureus: a new case in Spain]. | | 2011 | 21855180 |
| antibacterial activity of different honeys against pathogenic bacteria. | to study the antimicrobial activity of honey, 60 samples of various botanical origin were evaluated for their antimicrobial activities against 16 clinical pathogens and their respective reference strains. the microbiological quality of honeys and the antibiotic susceptibility of the various isolates were also examined. the bioassay applied for determining the antimicrobial effect employs the well-agar diffusion method and the estimation of minimum active dilution which produces a 1mm diameter in ... | 2011 | 21524711 |
| a type iv modification-dependent restriction enzyme sauusi from staphylococcus aureus subsp. aureus usa300. | a gene encoding a putative dna helicase from staphylococcus aureus usa300 was cloned and expressed in escherichia coli. the protein was purified to over 90% purity by chromatography. the purified enzyme, sauusi, predominantly cleaves modified dna containing 5mc and 5-hydroxymethylcytosine. cleavage of 5mc-modified plasmids indicated that the sites s5mcngs (s?=?c or g) are preferentially digested. the endonuclease activity requires the presence of adenosine triphosphate (atp) or datp whereas the ... | 2011 | 21421560 |
| defensin of the zebra mussel (dreissena polymorpha): molecular structure, in vitro expression, antimicrobial activity, and potential functions. | a 409 bp full length defensin gene was cloned and sequenced based on an expressed sequence tag (est) obtained from a normalized zebra mussel (dreissena polymorpha) foot cdna library developed in our laboratory. the d. polymorpha defensin (dpd) gene encoded a peptide with 76 amino acid residues. the mature dpd contains 54 amino acids with a fully functional insect defensin a domain. homologue searching against genbank database suggested that this dpd was phylogenetically close to defensins from a ... | 2010 | 20537393 |
| comprehensive characterization of methicillin-resistant staphylococcus aureus subsp. aureus col secretome by two-dimensional liquid chromatography and mass spectrometry. | two-dimensional lc combined with whole protein and peptide mass spectrometry is used to characterize proteins secreted by methicillin-resistant staphylococcus aureus col. protein identifications were accomplished via off-line protein fractionation followed by digestion and subsequent peptide analysis by reverse phase lc-esi-ltq-ft-ms/ms. peptide ms/ms analysis identified 127 proteins comprising 59 secreted proteins, seven cell wall-anchored proteins, four lipoproteins, four membrane proteins, an ... | 2010 | 20418541 |
| biological properties of the chilean native moss sphagnum magellanicum. | an ethanol extract prepared from the gametophyte chilean native moss sphagnum magellanicum was dried out, weighed and dissolved in distilled water. this extract was then assayed for its antibacterial activity against the g(-) bacteria azotobacter vinelandii, erwinia carotovora subsp. carotovora, enterobacter aerogenes, escherichia coli, pseudomonas aeruginosa, salmonella typhi, vibrio cholerae, and the g(+) bacteria staphylococcus aureus subsp. aureus, and streptococcus type beta. the growth of ... | 2009 | 19746269 |
| adhesion and detachment kinetics of several strains of staphylococcus aureus subsp. aureus under three different experimental conditions. | the kinetics of adhesion of five staphylococcus aureus subsp. aureus strains (cect 976, 4459, 4465, 4466 and 5191) to polypropylene at 25 degrees c in the absence of nutrients (pbs medium) were initially compared. those strains with the highest (cect 4459) and the lowest (cect 976) adhesion levels were selected for further studying the effects of a nutrient-rich adhesion-promoting medium (tsb plus 1% glucose-tsbg) as well as of a conditioning film consisting of dried mussel cooking juices (mcj) ... | 2007 | 17418309 |
| nonparallel secretion of antibacterial activity and protein in porcine pancreatic juice. | the antimicrobial activity of exocrine pancreatic juice is an important component of gastrointestinal tract innate defenses, yet little is known about whether secretion is regulated in parallel with digestive enzymes. in this study, we used 8 pigs with pancreatic catheters to quantify antibacterial activity and measure protein content (indicator of enzyme secretion) of pancreatic juice collected hourly from 0700 to 1900, with the animals adapted to being fed at 0800 and 1600. antibacterial activ ... | 2004 | 15028958 |
| a kinetic microplate method for quantifying the antibacterial properties of biological fluids. | the microplate assay for measuring antibacterial activity was adapted by incorporating a known concentration range of gentamicin as an internal standard. staphylococcus aureus subsp. aureus strain atcc 6538p, escherichia coli atcc 25922, and lactobacillus spp. were used as target organisms, although other indicator organisms and antibiotics can be examined. assay range and sensitivity were dependent on the species and density of indicator organism, and conditions (e.g., type, concentration, and ... | 2003 | 14529966 |
| isolation and characterization of a dna probe for staphylococcus aureus subspecies aureus biovar. | the gene encoding for polynucleotide phosphorylase (pnp) of a new biovar of staphylococcus aureus subsp. aureus (nbsa) has been isolated from a genomic library of strain m280(0). the coding region consisted of a 1094-bp hindiii-hindiii dna fragment encoding for a protein of 277 amino acids with a calculated molecular mass of 29.5 kda. the nucleotide sequence of the structural gene, contained a continuous open reading frame of 836 bp, showed significant homology with the genes of bacterial polynu ... | 1999 | 10079526 |
| coagulase-negative variants of methicillin-resistant staphylococcus aureus subsp. aureus strains isolated from hospital specimens. | at the department of clinical bacteriology of medical university of warsaw, s. aureus strains displaying negative reactions in the coagulase tube-test were isolated with a relatively high frequency from clinical specimens. as many as seventeen of such strains all from different patients were isolated during 1994 among mrsa strains. despite coagulase negativity all strains were positive in tests for clumping factor (cf), protein a and thermonuclease. biochemical characteristics, phage patterns an ... | 1998 | 9861681 |
| the polyvalent staphylococcal phage phi 812: its host-range mutants and related phages. | ninety-five percent of 782 culture collection strains, as well as hospital strains of staphylococcus aureus subsp. aureus of different provenance and 43% of 89 culture collection strains of different coagulase-negative species of the genus staphylococcus, were found to be sensitive to the polyvalent phage phi 812 or to at least one of its host-range mutants or to the polyvalent phages sk311, phi 131, and u16. thus sensitivity to the polyvalent staphylococcal phages seems to be one of the common ... | 1998 | 9657943 |
| [antimicrobial activities of clavulanic acid/amoxicillin against freshly isolated clinical strains from outpatients]. | in order to investigate antimicrobial activities of clavulanic acid/amoxicillin (cva/ampc) against freshly isolated clinical strains obtained in 1995, beta-lactamase activities and minimum inhibitory concentration (mics) were determined including those of the control drugs. the results are summarized as follows; 1. detection frequencies of beta-lactamase producing strains were as follows: methicillin-susceptible staphylococcus aureus subsp. aureus (mssa, 90.0%), haemophilus influenzae (22.0%), m ... | 1995 | 8587166 |
| molecular characterization of a catalase-negative staphylococcus aureus subsp. aureus strain collected from a patient with mitral valve endocarditis and pericarditis revealed a novel nonsense mutation in the kata gene. | we report a case of endocarditis and pericarditis caused by catalase-negative staphylococcus aureus. molecular characterization revealed a novel nonsense mutation in the kata gene, leading to a loss of 238 amino acids (47% of the wild-type catalase protein), including the heme-binding site, nadph-binding region, and tyr-337, essential for catalysis. | 2011 | 21715584 |