| prevalence of staphylococcus species during the periparturient period in primiparous and multiparous cows. | during a 14-mo period, 77 multiparous and 36 primiparous cows were sampled to determine the prevalence of staphylococci during the periparturient period. distal streak canal swabs were taken at 14 d prepartum, and foremilk was sampled the first 5 consecutive wk of lactation. staphylococcus aureus was isolated from 7.6% of quarters of primiparous cows but from only .6% of quarters of multiparous cows at parturition. prevalence in primiparous cows declined to 3.5% by the wk-1 sampling. quarter pre ... | 1992 | 1500579 |
| hemolytic interactions of dermatophilus congolensis. | the strains of dermatophilus congolensis grew on blood agar with washed sheep erythrocytes with marked total hemolysis. in testing for hemolytic interactions they gave a significant synergistic effect of a characteristic shape with rhodococcus equi and streptococcus agalactiae, whereas with staphylococcus aureus producing beta hemolysin and with staphylococcus aureus producing delta hemolysin a simultaneous synergistic as well as antagonistic effect were observed. first of all a conspicuous inhi ... | 1992 | 1621476 |
| prevalence of intramammary infection and teat canal colonization in unbred and primigravid dairy heifers. | teat canal keratin (n = 461) and mammary gland secretions (n = 370) were collected from 31 unbred and 85 primigravid jersey heifers from one research and three commercial dairy herds. of 97 heifers from which secretion samples were obtained, 96.9% had intramammary infections and 29% showed clinical symptoms. seventy-five percent of quarters were infected. staphylococcus aureus were isolated from 36 (37.1%) heifers and 55 (14.9%) quarters. one hundred and eight (93.1%) heifers and 326 (70.7%) qua ... | 1990 | 1690231 |
| encapsulation of coagulase-negative staphylococci of bovine origin. | capsule expression was assessed in six coagulase-negative staphylococcal strains in serum-soft agar and by india ink and electron microscopy. classification of strains as encapsulated by serum-soft agar and india ink methods differed. staphylococcus chromogenes, staph. hyicus, and staph. simulans grew as diffuse colonies in serum-soft agar and unstained halos were detected in india ink preparations. staphylococcus hominis and staph. simulans grew as diffuse colonies in serum-soft agar but no uns ... | 1991 | 1716624 |
| effect of naturally occurring coagulase-negative staphylococci infections on new infections by mastitis pathogens in the bovine. | microbiological data from 1123 uninfected quarters and 216 quarters with preexisting coagulase-negative staphylococci infections were analyzed to determine the influence of infection status on subsequent new infection rate. overall, prevalence of new infections in uninfected quarters was approximately two times that in quarters already harboring a coagulase-negative staphylococcus infection. new infections by coagulase-negative staphylococci were greater in uninfected quarters than in quarters w ... | 1991 | 1894794 |
| comparative studies on bacteriolytic properties of staphylococcus chromogenes and staphylococcus hyicus. | staphylococcus chromogenes and staphylococcus hyicus showed bacteriolytic activities towards a micrococcus luteus reference strain. this was demonstrated on tryptone soya agar containing m. luteus cells. both bacteriolytic enzymes could be isolated by ionic exchange chromatography and subsequent gel filtration. the isolated bacteriolysin of s. chromogenes lysed the m. luteus reference culture, was heat inactivated by 95 degrees c and precipitated specifically with antiserum produced against the ... | 1991 | 1950253 |
| evaluation of the staph-zym system with staphylococci isolated from bovine intramammary infections. | a total of 148 staphylococci isolated from bovine intramammary infections were used to evaluate the staph-zym system (rosco, taastrup, denmark). the overall accuracy of the system was 91.9%. the system correctly identified all strains of staphylococcus aureus, staphylococcus simulans, and staphylococcus xylosus and 95% of staphylococcus intermedius strains. of 33 staphylococcus hyicus strains, 31 (93.9%) were classified correctly by the staph-zym system, as well as 8 (80%) of 10 staphylococcus c ... | 1991 | 1993769 |
| expression of glycocalyx by coagulase-negative staphylococcus species isolated from bovine milk. | two hundred and six strains of coagulase-negative staphylococcus species were assessed for expression of glycocalyx on serum soft agar, india ink and adherence techniques. the organisms were maintained on trypticase soy agar plates at 4 degrees c for 30 d (120 strains) or stored at -80 degrees c in skim milk for 90 d (60 strains). additionally, 26 milk samples from cows known to have excreted coagulase-negative staphylococci were used to inoculate serum soft agar directly. nine of 26 direct cult ... | 1991 | 2030097 |
| a convenient method for differentiation of coagulase-negative staphylococci isolated from bovine mammary glands. | the utility of trehalose-mannitol broth and arabinose-cellobiose broth for identification of staphylococcus epidermidis and novobiocin-resistant staphylococci was determined using 236 coagulase-negative staphylococci isolated from bovine mammary glands. none of the 49 s. epidermidis strains was positive in trehalose-mannitol broth; whereas, all strains of staphylococcus hyicus, staphylococcus chromogenes, staphylococcus haemolyticus, staphylococcus hominis, staphylococcus warneri, and staphyloco ... | 1991 | 2045549 |
| protective effect of staphylococcus chromogenes infection against staphylococcus aureus infection in the lactating bovine mammary gland. | the susceptibility of uninfected or staphylococcus chromogenes-infected quarters to challenge with staphylococcus aureus was measured. seventeen s. chromogenes-infected quarters were challenged by infusion of s. aureus into the teat sinus; 47% (8 of 17) became infected and all 18 uninfected quarters challenged similarly with s. aureus became infected. no differences in daily milk yield were seen between uninfected quarters and s. chromogenes-infected quarters prior to s. aureus infusion. postinf ... | 1990 | 2099367 |
| [coagulase negative staphylococci isolated from cow milk]. | the species composition was determined in the set of 52 randomly selected strains of coagulase-negative staphylococci, which were isolated from the milk of dairy cows in 1989. of this set of strains, the following species were identified: staphylococcus hyicus subsp. chromogenes (26.9% strains of the set), s. hyicus subsp. hyicus (10.3%), s. xylosus (19.3%), s. saprophyticus (11.5%), s. warneri (9.6%), s. haemolyticus (9.6%), s. hominis (3.8%). attention is drawn to the increasing occurrence and ... | 1990 | 2100424 |
| enterotoxin production by staphylococci isolated from healthy goats. | the ability of 342 staphylococcal isolates from different anatomical sites in healthy goats to produce staphylococcal enterotoxins (se) was investigated. se were produced by 74.3% of the 70 coagulase-positive strains and by 22% of the coagulase-negative strains studied. most enterotoxigenic strains were isolated from the skin of udders and teats and from milk. sec was the se type most frequently produced, either alone (67.9%) or in combination with others. five coagulase-negative species not pre ... | 1990 | 2339886 |
| characteristic bacteriolytic activities of staphylococcus hyicus. | staphylococcus hyicus demonstrated characteristic bacteriolytic activities towards a micrococcus luteus reference strain. this lytic activity was demonstrated on medium containing m. luteus cells as large zones of transparency around the culture streak. smaller zones of transparency were observed with staphylococcus intermedius, staphylococcus chromogenes, and some strains of staphylococcus aureus but not with other coagulase-negative staphylococcal species. the distribution and extent of the ba ... | 1989 | 2768457 |
| interaction of sub-epithelial connective tissue components with staphylococcus aureus and coagulase-negative staphylococci from bovine mastitis. | staphylococcus aureus and coagulase-negative staphylococci (cns) isolated from bovine mastitis were examined for their ability to interact with 125i-labelled fibronectin, fibrinogen and type ii collagen. their relative surface hydrophobicity and production of extracellular capsule were also investigated. almost all s. aureus strains bound fibronectin (mean value 23%), fibrinogen (mean value 12%) and type ii collagen (mean value 16%). cns bound fibronectin (mean value 6%) and type ii collagen (me ... | 1988 | 3218077 |
| synergistic hemolysis associated with coagulase-negative staphylococci isolated from bovine mammary glands. | a total of 353 coagulase-negative staphylococcus (cns) isolates from infected bovine mammary glands were tested for cytolysin production by using the synergistic hemolysin assay (sha). overall, 34.6% of cns isolates were sha positive. human-associated, coagulase-negative staphylococcal species contained the greatest number of sha-positive strains. milk leukocyte levels expressed as somatic cell counts (scc) were elevated with sha-positive staphylococcus epidermidis, staphylococcus hominis, and s ... | 1987 | 3693535 |
| biotypes of staphylococcus epidermidis and micrococcus organisms, isolated from intramammary infections, reclassified into species of the genus staphylococcus (epidermidis, hyicus, xylosus, and sciuri). | in a previous report (5), strains of staphylococcus epidermidis and micrococcus isolated from bovine intramammary infections were classified by the baird-parker (b-p) system and by serologic typing of the proteolytic enzymes. since then, newer methods for distinguishing the genera staphylococcus and micrococcus and for defining new species have been reported. by utilizing these methods, the organisms from the original study were reclassified as follows: s. epidermidis b-p subgroup ii, proteinase ... | 1983 | 6340953 |
| evaluation of the staph-ident and staphase systems for identification of staphylococci from bovine intramammary infections. | the staph-ident and staphase systems (analytab products, plainview, n.y.) were compared with conventional methods for identification of staphylococci isolated from bovine intramammary infections. adjunct testing by colony morphology, pigmentation, and biochemical tests was conducted to resolve discrepant identifications. the initial accuracies of the conventional scheme and staph-ident were 92.1 and 89.2%, respectively. staphylococcus hyicus subsp. chromogenes could not be identified by means of ... | 1984 | 6386862 |
| mastitis in dairy heifers: initial studies on prevalence and control. | initial studies to determine the prevalence of mastitis in heifers of breeding age and in pregnant dairy heifers demonstrated that imi were present in 97% of heifers and 75% of quarters. the most common isolates were staphylococcus aureus, staphylococcus hyicus, and staphylococcus chromogenes; scc ranged from 12.4 to 17.3 x 10(6)/ml. approximately 29% of heifers and 15% of quarters exhibited clinical mastitis at breeding age, as evidenced by clots or flakes in mammary secretions. histologic exam ... | 1995 | 7593856 |
| use of liposome-immunopotentiated exopolysaccharide as a component of an ovine mastitis staphylococcal vaccine. | experiments on the development of a vaccine against staphylococcal mastitis were carried out in ewes. the vaccine (spanish patent no. 9200223) has the following components: (i) inactivated (formalinized) bacteria (staphylococcus aureus and a coagulase-negative staphylococcal species. staphylococcus simulans) and s. aureus toxoid in presence of an adjuvant (dextran sulfate, mw 500,000); and (ii) s. aureus exopolysaccharide included within liposomes. high serum antibody titres were obtained agains ... | 1994 | 8165857 |
| reversible enzymic protection of the alpha-amino group of amino acid derivatives using an aminopeptidase a. | in previous papers we have reported that an aminopeptidase a (ec 3.4.11.7) purified from staphylococcus chromogenes was able to catalyse the introduction of l-malic acid at the n-terminus of tyr and phe derivatives. we now show that this enzyme can be used for selective alpha-amino protection of derivatives of probably all amino acids, except gly and pro, by the malyl group. the following l-malyl derivatives were synthesized in thermodynamically controlled reactions with yields ranging from 4 to ... | 1993 | 8397888 |
| purification and characterization of an aminopeptidase a from staphylococcus chromogenes and its use for the synthesis of amino-acid derivatives and dipeptides. | an aminopeptidase with original specificity was purified 3800-fold to homogeneity from a cellular extract of staphylococcus chromogenes. the enzyme was specific for acidic amino acids (asp and glu) at the n-terminus of peptides and thus can be classified as an aminopeptidase a. however, its specificity was not restricted to acidic amino acids: alpha-hydroxy acids such as l-malic and l-lactic acids were also accepted in position p1. the enzyme had a broad specificity for the residue at position p ... | 1993 | 8425520 |
| characteristics of coagulase-negative staphylococci isolated from bovine intramammary infections. | coagulase-negative staphylococci (cns) isolated from 86 different bovine intramammary infections (imi) were investigated for their plasmid content, antimicrobial resistance, and infection characteristics. plasmids were isolated from 30.2% of cns. number of plasmid bands ranged from 1 to 5. with the exception of tetracycline resistance, the presence of plasmids was not related to antibiotic resistance. staphylococcus chromogenes was the cns most frequently isolated from bovine imi. intramammary i ... | 1993 | 8506610 |
| effects of freezing on the viability of nine pathogens from quarters with subclinical mastitis. | milk samples from 45 quarters containing mastitis pathogens were collected from lactating cows to determine the viability of those pathogens after freezing. an initial bacteria count was conducted, and samples were divided into 2-ml portions and frozen. weekly bacteria counts were conducted for 6 wk. viability after freezing was determined on five isolates of nine bacterial species: staphylococcus aureus, staphylococcus hyicus, staphylococcus chromogenes, staphylococcus xylosus, streptococcus ag ... | 1996 | 8708092 |
| characterization of non-pigmented staphylococcus chromogenes. | a large number of non-pigmented staphylococcus chromogenes were isolated from the skin of piglets with exudative epidermitis and healthy pigs. their characteristics were homologous with s. chromogenes type strain, except for pigment production. strains of non-pigmented s. chromogenes exhibited high levels of dna homology with s. chromogenes type strain. the colony morphology and pigmentation of non-pigmented s. chromogenes was very similar to s. hyicus type strain, but their characteristics diff ... | 1996 | 8844615 |
| diversity of adenine conversion in staphylococci. | the special position of staphylococcus intermedius in comparison with staphylococcus saprophyticus, staphylococcus chromogenes, and staphylococcus hyicus was shown by deamination of adenine to hypoxanthine. s. chromogenes and s. hyicus converted adenine to adenosine. estimation of adenine conversion may be used for differentiation of s. intermedius from other coagulase-positive staphylococci. | 1996 | 8893524 |
| prevalence and duration of intramammary infection in danish heifers during the peripartum period. | milk samples were collected from 180 heifers from 20 herds around parturition to study the prevalence and persistence of intramammary infection (imi). a total of 3382 quarter milk samples was collected each week from 4 wk before parturition to 4 wk after parturition. staphylococcus chromogenes, the most commonly found bacterial species before parturition, was isolated from 15% of all quarters. however, imi with staph. chromogenes decreased shortly after parturition to around 1% of quarters. infe ... | 1997 | 9058272 |
| molecular properties of the erythromycin resistance plasmid ppv141 from staphylococcus chromogenes. | the 2.3-kb erythromycin resistance (emr) plasmid ppv141 of staphylococcus chromogenes 3688 was isolated and characterized. nucleotide sequence analysis identified orf1 and orf2 separated by a 445-bp spacing, encoding a 158-residue replication protein (rep141) and a 244-residue erythromycin resistance protein (erm, rrna adenine n-6-methyltransferase), respectively. structural analysis and southern hybridization showed that the rep and ermm genes in ppv141 shared homology with other known emr plas ... | 1997 | 9169203 |
| isolation of staphylococcus chromogenes from an unusual case of impetigo in a goat. | | 1997 | 9194303 |
| pulsed-field gel electrophoresis of staphylococcus hyicus and staphylococcus chromogenes genomic dna and its taxonomic, epidemiologic and ecologic applications in veterinary medicine. | one hundred and thirty-eight strains of staphylococcus hyicus and 21 strains of s. chromogenes isolated from animals were analyzed by pulsed-field gel electrophoresis (pfge) after restriction endonuclease smal digestion of chromosomal dna. eighty-eight strains of s. hyicus from pigs with or without exudative epidermitis (ee) generated 16 to 26 fragments in the size range of < 1 to 485 kb, and yielded 39 different patterns. with regard to the strains from pigs with ee, pfge patterns differed acco ... | 1997 | 9234218 |
| cleavage of influenza a virus h1 hemagglutinin by swine respiratory bacterial proteases. | cleavage of influenza a virus hemagglutinin (ha) is required for expression of fusion activity and virus entry into cells. extracellular proteases are responsible for the proteolytic cleavage activation of avirulent avian and mammalian influenza viruses and contribute to pathogenicity and tissue tropism. the relative contributions of host and microbial proteases to cleavage activation in natural infection remain to be established. we examined 23 respiratory bacterial pathogens and 150 aerobic ba ... | 1997 | 9311838 |
| identification of staphylococcal and streptococcal causes of bovine mastitis using 16s-23s rrna spacer regions. | bovine mastitis is caused mainly by certain staphylococcus and streptococcus species. the sequences of the 16s-23s rrna spacer regions were determined for the nine species which cause mastitis: staphylococcus aureus, staphylococcus chromogenes, staphylococcus epidermidis, staphylococcus hyicus, staphylococcus simulans, staphylococcus xylosus, streptococcus agalactiae, streptococcus dysgalactiae and streptococcus uberis. significant variation was found between the spacer sequences of different sp ... | 1997 | 9387227 |
| identification of staphylococcus species by 16s-23s rdna intergenic spacer pcr analysis. | to investigate whether 16s-23s rdna (rdna) spacer region length polymorphisms are suitable for the identification of staphylococcus strains, the 16s-23s rdna intergenic spacer region lengths of 221 strains belonging to 31 species were studied by using a pcr-based method. each species presented a specific 16s-23s pattern made of 1-8 fragments ranging from 104-771 bp, with the exception of the species staphylococcus warnei, staphylococcus caprae and staphylococcus piscifermentans, which presented ... | 1998 | 9734063 |
| bacteria associated with clinical mastitis in dairy heifers. | a 1-yr field investigation of clinical mastitis in heifers was carried out in 24 veterinary districts in norway. quarter lacteal secretions from cases that occurred prepartum or within 14 d postpartum were examined bacteriologically. the study included 1040 heifers with clinical mastitis, and the total number of quarters that were clinically affected was 1361. the organisms that were most frequently isolated from samples from these quarters were staphylococcus aureus (44.3%), streptococcus dysga ... | 1999 | 10212457 |
| bacteriological studies of blood, tissue fluid, lymph and lymph nodes in patients with acute dermatolymphangioadenitis (dla) in course of 'filarial' lymphedema. | filarial lymphedema is complicated by frequent episodes of dermatolymphangioadenitis (dla). severe systemic symptoms during attacks of dla resemble those of septicemia. the question we asked was whether bacterial isolates can be found in the peripheral blood of patients during the episodes of dla. out of 100 patients referred to us with 'filarial' lymphedema 14 displayed acute and five subacute symptoms of dla. all were on admission blood microfilariae negative but had a positive test in the pas ... | 1999 | 10546838 |
| cytotoxic activity of coagulase-negative staphylococci in bovine mastitis. | secreted toxins play important roles in the pathogenesis of bacterial infections. in this study, we examined the presence of secreted cytotoxic factors of coagulase-negative staphylococci (cons) from bovine clinical and subclinical mastitis. a 34- to 36-kda protein with cell-rounding cytotoxic activity was found in many cons strains, especially in staphylococcus chromogenes strains. the protein caused cell detachment and cell rounding in several cell lines, including hep-2, int 407, cho-k1, and ... | 2000 | 10678913 |
| identification and antimicrobial susceptibility of staphylococcus chromogenes isolates from intramammary infections of dairy cows. | staphylococcus chromogenes is a highly prevalent species in subclinical mastitis with a well-established impact on somatic cell count. few data are available on its antimicrobial susceptibility. the objective of this study was three-fold: (1) to evaluate simple identification tests by comparing them with a genomic method; (2) to determine minimal inhibitory concentrations (mics) of different antibiotics; (3) to search for the presence of important resistance mechanisms and resistance-determining ... | 2002 | 12034545 |
| prepartum teat apex colonization with staphylococcus chromogenes in dairy heifers is associated with low somatic cell count in early lactation. | a high number of dairy heifers freshen with udder health problems. the prevalence of teat apex colonization (tac) with staphylococcus chromogenes, one of the most widespread coagulase-negative staphylococci (cns) in milk samples from freshly calved dairy heifers, was measured cross-sectionally in non-lactating heifers on eight commercial dairy farms in belgium. the influence of age on this prevalence, and the association between teat apex colonization with s. chromogenes prepartum and quarter mi ... | 2003 | 12523986 |
| occurrence of resistance to antibiotics, metals, and plasmids in clinical strains of staphylococcus spp. | presence of staphylococci resistant to antibiotics is of concern, as is the possible spread of resistance determinants among staphylococcus species. during the past several years, a series of staphylococcal isolates that demonstrated reduced susceptibility to vancomycin or other glycopeptides has been reported. however, there are no useful data on species sensitivity of coagulase-negative staphylococci to heavy metals. | 2003 | 12700009 |
| experimentally induced teat stenosis in dairy ewes: clinical, pathological and ultrasonographic features. | a strain of staphylococcus chromogenes was introduced into the teat cistern of five ewes, teat inflammation and stenosis being the primary consequences. initially, the inoculated teats were swollen and warm; later, a hard structure was palpated running lengthwise inside the teat, with a thick ring above the tip of the teat, which interfered with expression of milk. mastitis, confirmed by clinical, cytological, bacteriological and histological findings, was evident 4 days after infection. ultraso ... | 2004 | 14693127 |
| purification and characterization of a novel staphylococcus chromogenes exfoliative toxin. | | 2004 | 15107037 |
| in vitro growth inhibition of major mastitis pathogens by staphylococcus chromogenes originating from teat apices of dairy heifers. | earlier field observations suggest that teat apex colonization by staphylococcus chromogenes pre-partum in dairy heifers protects udder quarters against elevated somatic cell counts early post-partum. to explain these findings, the in vitro inhibitory capability of s. chromogenes from teat apices of heifers towards some major mastitis pathogens was tested using a modified cross-streaking method. two out of 10 s. chromogenes isolates, both originating from two different teats from the same heifer ... | 2004 | 15223126 |
| exudative epidermitis in pigs caused by toxigenic staphylococcus chromogenes. | staphylococcus chromogenes is closely related to staphylococcus hyicus, which is recognised as the causative agent of exudative epidermitis (ee) in pigs. s. chromogenes is part of the normal skin flora of pigs, cattle and poultry and has so far been considered non-pathogenic to pigs. a strain of s. chromogenes producing exfoliative toxin type b, exhb, was identified by the use of a multiplex pcr specific for the exfoliative toxins from s. hyicus. the exfoliative toxin from s. chromogenes reacted ... | 2005 | 15708827 |
| bap-dependent biofilm formation by pathogenic species of staphylococcus: evidence of horizontal gene transfer? | the biofilm-associated protein (bap) is a surface protein implicated in biofilm formation by staphylococcus aureus isolated from chronic mastitis infections. the bap gene is carried in a putative composite transposon inserted in sapibov2, a mobile staphylococcal pathogenicity island. in this study, bap orthologue genes from several staphylococcal species, including staphylococcus epidermidis, staphylococcus chromogenes, staphylococcus xylosus, staphylococcus simulans and staphylococcus hyicus, w ... | 2005 | 16000737 |
| assessment of accuracy of disk diffusion tests for the determination of antimicrobial susceptibility of common bovine mastitis pathogens: a novel approach. | a novel approach was used to assess disk diffusion accuracy for determination of antibiotic susceptibility of various bovine mastitis pathogens (escherichia coli, staphylococcus aureus, staphylococcus chromogenes, and streptococcus dysgalactiae). mic and disk diffusion diameters were compared for 587 bovine mastitis bacterial isolates collected in israel and 3,186 drug-organism combinations. results were analyzed by roc curves, bayesian statistics, and standard descriptive methods. low correlati ... | 2005 | 16359193 |
| identification of biochemically atypical staphylococcus aureus clinical isolates with three automated identification systems. | between january and april 2002, a total of 271 strains of staphylococcus aureus were isolated from clinical specimens at toho university omori hospital, japan, including 201 (74.2 %) which were identified as meticillin-resistant s. aureus (mrsa). however, 34 (12.5 %) were biochemically atypical, because they did not produce acid on mannitol salt agar or did not agglutinate in staphaurex testing but were categorized as mrsa by pcr analysis and by antibiotic susceptibility. three automatic identif ... | 2006 | 16533985 |
| species distribution and properties of staphylococci from canine dermatitis. | the occurrence and phenotypic, and genotypic properties of 24 staphylococcus isolates from canine dermatitis were investigated. the predominant staphylococcal species was staphylococcus intermedius. the other species such as staphylococcus chromogenes, staphylococcus sciuri, staphylococcus aureus, staphylococcus saprophyticus, staphylococcus epidermidis, and staphylococcus capitis were only occasionally isolated. the study showed low level biochemical diversity among s. intermedius isolates. res ... | 2007 | 17126372 |
| staphylococcal and micrococcal adherence to canine and feline corneocytes: quantification using a simple adhesion assay. | in this paper a simple adhesion assay suitable for the assessment of bacterial adhesion to both canine and feline corneocytes is described. using this assay staphylococcus intermedius, staphylococcus aureus and staphylococcus chromogenes were shown to adhere well to both canine and feline corneocytes. the numbers of adherent bacteria were, however, generally lower for feline corneocytes. both staphylococcus hominis and a micrococcus species adhered poorly to canine and feline corneocytes. this i ... | 2007 | 17222237 |
| [sensitivity to various antibiotics of coagulase-negative staphylococci isolated from samples of milk from dutch dairy cattle]. | during recent years the prevalence of coagulase-negative staphylococci in milk samples from dutch dairy cows has increased. in 1999 16.2% of the bacteria isolated from milk collected from cows with subclinical mastitis were coagulase-negative staphylococci. in 2004 this proportion was 42.2%. the proportion of coagulase-negative staphylococci of the bacteria isolated from milk samples from cows with clinical mastitis was 7.3% in 1999 and 14.1% in 2004. in this study, the susceptibility of 108 coa ... | 2007 | 17436810 |
| antibiotic resistance patterns of coagulase-negative staphylococcus strains isolated from blood cultures of septicemic patients in turkey. | the aim of this study is to determine antibiotic resistance patterns and slime production characteristics of coagulase-negative staphylococci (cons) caused nosocomial bacteremia. a total of 200 cons strains were isolated from blood samples of patients with true bacteremia who were hospitalized in intensive care units and in other departments of istanbul university cerrahpasa medical hospital between 1999 and 2006. among 200 cons isolates, staphylococcus epidermidis was the most prevalent species ... | 2009 | 17475456 |
| bovine intramammary infections caused by coagulase-negative staphylococci may persist throughout lactation according to amplified fragment length polymorphism-based analysis. | persistence of coagulase-negative staphylococci (cns) in intramammary infections during lactation was studied in a research dairy herd of university of helsinki. milk samples from 328 udder quarters of 82 dairy cows (30 primiparous, 52 multiparous) were collected 2 wk before calving, at calving, and every 4 wk thereafter until the end of lactation or until the cow left the herd. the cns isolated from the milk samples were analyzed with the api staph id 32 (biomérieux, marcy l'etoile, france) tes ... | 2007 | 17582115 |
| identification of coagulase-negative staphylococci from bovine mastitis using rflp-pcr of the groel gene. | coagulase-negative staphylococci (cns) have become the predominant pathogens causing bovine mastitis in many countries. cns infections are associated with damage to milk secretory tissue of the mammary gland by increased connective tissue stroma, moderate increases of somatic cells count in milk and significant production decreases. these consequences impose serious economic losses for the farmers and the dairy industry. routine veterinary laboratories do not usually identify cns at the species ... | 2008 | 18295414 |
| [aerobic bacterial flora from the digestive tract of the common vampire bat, desmodus rotundus (chiroptera: phyllostomidae)]. | this study addresses the composition of microbial flora in the vampire bat (desmodus rotundus) primarily because all available data are outdated, and because of the economical significance of this bat species. twenty-one bats were collected and their aerobic bacteria documented separately for stomach and intestine. bacteria were identified through the analytical profile index (api), and results analyzed with the apilab software. a total of thirty bacterial species were isolated from sixteen fema ... | 2006 | 18491612 |
| coagulase-negative staphylococci-emerging mastitis pathogens. | coagulase-negative staphylococci (cns) have become the most common bovine mastitis isolate in many countries and could therefore be described as emerging mastitis pathogens. the prevalence of cns mastitis is higher in primiparous cows than in older cows. cns are not as pathogenic as the other principal mastitis pathogens and infection mostly remains subclinical. however, cns can cause persistent infections, which result in increased milk somatic cell count (scc) and decreased milk quality. cns i ... | 2009 | 18848410 |
| host response in bovine mastitis experimentally induced with staphylococcus chromogenes. | an experimental infection model was developed to study host response to intramammary infection in cows caused by staphylococcus chromogenes. cns intramammary infections have become very common in modern dairy herds, and they can remain persistent in the mammary gland. more information would be needed about the pathophysiology of cns mastitis, and an experimental mastitis model is a means for this research. six primiparous holstein-friesian cows were challenged with s. chromogenes 4 weeks after c ... | 2009 | 18938048 |
| prevalence and persistence of coagulase-negative staphylococcus species in three dairy research herds. | coagulase-negative staphylococcus species (cns) were isolated from 11.3% (1407 of 12,412) of mammary quarter milk samples obtained from cows in three dairy research herds in 2005. approximately 27% (383/1407) of cns was identified to the species level. the species distribution among those cns identified from all herds was staphylococcus chromogenes (48%), staphylococcus hyicus (26%), staphylococcus epidermidis (10%), staphylococcus simulans (7%), staphylococcus warneri (2%), staphylococcus homin ... | 2009 | 18950962 |
| antimicrobial susceptibility of coagulase-negative staphylococcus species isolated from bovine milk. | coagulase-negative staphylococcus (cns) isolates (n=168) obtained from milk from heifers and dairy cows were screened for minimum inhibitory concentration (mic) to antimicrobials used commonly for mastitis therapy. of the 10 cns species included in the study, the predominant species were staphylococcus chromogenes (n=61), staphylococcus epidermidis (n=37), staphylococcus hyicus (n=37), and staphylococcus simulans (n=16). the majority of cns was susceptible to ampicillin, oxacillin, cephalothin, ... | 2009 | 18950969 |
| control of heifer mastitis: antimicrobial treatment-an overview. | initial studies in louisiana, usa to determine the prevalence of mastitis in breeding age dairy heifers demonstrated that intramammary infections (imis) were present in 97% of heifers and 75% of quarters. most common isolates were staphylococcus aureus, staphylococcus hyicus, and staphylococcus chromogenes; somatic cell counts (sccs) ranged from 12.4 to 17.3 x 10(6)ml(-1). histologic examination of staph. aureus-infected quarters demonstrated significant reductions in alveolar epithelial and lum ... | 2009 | 18963599 |
| prevalence and herd-level risk factors for intramammary infection with coagulase-negative staphylococci in dutch dairy herds. | in this study, the prevalence of intramammary infection (imi) with coagulase-negative staphylococci (cns) in the netherlands was estimated on 49 randomly selected herds with at least 40 lactating cows. in total, 4220 quarter milk samples were collected. the prevalence of cns imi in the netherlands was estimated at 10.8% at quarter level and 34.4% at cow level, making it the most frequently isolated group of pathogens. fourteen species of cns were identified; the most frequently isolated species ... | 2009 | 18977613 |
| performance of api staph id 32 and staph-zym for identification of coagulase-negative staphylococci isolated from bovine milk samples. | in this study, the accuracy of two phenotypic tests, api staph id 32 and staph-zym, was determined for identification of coagulase-negative staphylococci (cns) from bovine milk samples in comparison with identification based on dna-sequencing. a total of 172 cns isolated from bovine milk were classified into 17 species. the most frequently isolated species based on rpob sequencing were staphylococcus chromogenes and staphylococcus epidermidis, followed by staphylococcus xylosus, staphylococcus w ... | 2009 | 19110383 |
| effect of bovine lactoferrin on the internalization of coagulase-negative staphylococci into bovine mammary epithelial cells under in-vitro conditions. | coagulase-negative staphylococci (cns) have emerged as bovine mastitis pathogens in many countries. cns mastitis is generally mild but can persist in the udder for long periods. pathogenesis of cns intramammary infection is not well understood. in the present study, adhesion, invasion and intracellular replication of twenty-two cns strains isolated from bovine mastitis and the effect of bovine lactoferrin (blf) on the internalization were studied in vitro in a bovine mammary epithelial (bme) cel ... | 2009 | 19121234 |
| [unanticipated outbreak of q fever during a study using sheep, and its significance for further projects]. | effective measures for protection of laboratory staff against infections due to experiments with pathogens are a challenge for safeguarding against hazards. investigations on limited epidemic infections and their causes are of high relevance. during an experiment with pregnant sheep 5 of 7 persons who took part in the operation procedures developed q fever 10 to 24 days after exposure. one sheep aborted spontaneously 3 days after the third experiment. examinations of the aborted material reveale ... | 2009 | 19226931 |
| response to somatic cell count-based selection for mastitis resistance in a divergent selection experiment in sheep. | a divergent selection experiment in sheep was implemented to study the consequences of log-transformed somatic cell score (scs)-based selection on resistance to natural intramammary infections. using dams and progeny-tested rams selected for extreme breeding values for scs, we created 2 groups of ewes with a strong divergence in scs of approximately 3 genetic standard deviations. a survey of 84 first-lactation ewes of both the high and low scs lines indicated favorable responses to scs-based sel ... | 2009 | 19233814 |
| staphylococcus massiliensis sp. nov., isolated from a human brain abscess. | gram-positive, catalase-positive, coagulase-negative, non-motile, non-fermentative and novobiocin-susceptible cocci were isolated from a human brain abscess sample (strain 5402776(t)). this novel strain was analysed by a polyphasic taxonomic approach. the respiratory quinones detected were mk-7 (93 %) and mk-6 (7 %) and the major fatty acids were c(15 : 0) iso (60.5 %), c(17 : 0) iso (8.96 %) c(15 : 0) anteiso (7.93 %) and c(19 : 0) iso (6.78 %). the peptidoglycan type was a3alpha l-lys-gly(2-3) ... | 2010 | 19666814 |
| bovine subclinical mastitis caused by different types of coagulase-negative staphylococci. | subclinical mastitis caused by intramammary infections (imi) with coagulase-negative staphylococci (cns) is common in dairy cows and may cause herd problems. control of cns mastitis is complicated by the fact that cns contain a large number of different species. the aim of the study was to investigate the epidemiology of different cns species in dairy herds with problems caused by subclinical cns mastitis. in 11 herds, udder quarter samples were taken twice 1 mo apart, and cns isolates were iden ... | 2009 | 19762813 |
| identification of first exfoliative toxin in staphylococcus pseudintermedius. | staphylococcus aureus, staphylococcus hyicus, and staphylococcus chromogenes are known to cause skin infections in human or animals by producing exfoliative toxins (ets). staphylococcus pseudintermedius can also cause canine pyoderma, but no exfoliative toxins or similar toxins have been reported. pcr with degenerate primers targeted to the conserved regions in eta, etb, and etd from s. aureus and shetb from s. hyicus, and subsequent chromosome walking identified a novel gene, designated as exi ... | 2009 | 19891731 |
| staphylococcus chromogenes-induced folliculitis in goat kids. | | 2010 | 20190219 |
| high concentration of human lactoferrin in milk of rhlf-transgenic cows relieves signs of bovine experimental staphylococcus chromogenes intramammary infection. | six transgenic cows producing recombinant human lactoferrin (rhlf) in their milk and five normal cows at the same lactation stage were experimentally infected with staphylococcus chromogenes to study the effect of a high concentration of lactoferrin in milk. coagulase-negative staphylococci such as s. chromogenes have become very common as agents causing mild or subclinical mastitis. all transgenic cows became infected but showed no clinical signs, unlike the control cows, which developed mild c ... | 2010 | 20400186 |
| study of microbial contamination and dosing accuracy of oral dispensers. | the optimal administration of liquid medications requires accurate dose delivery. this is particularly important in the treatment of infants and children, as well as elderly people, who are more sensitive to dosage errors. previous studies revealed significant dosage inaccuracies with measuring spoons. oral syringes are therefore generally recommended instead. there is no data on the efficacy of standard cleaning techniques, and consequently on the degree of microbial contamination associated wi ... | 2010 | 20831530 |
| volatile and biogenic amines, microbiological counts, and bacterial amino acid decarboxylase activity throughout the salt-ripening process of anchovies (engraulis encrasicholus). | chemical and microbiological parameters were studied during the industrial production of salt-ripened anchovies (engraulis encrasicholus). gradual acidification and increases in the proteolysis index and in total volatile basic nitrogen were observed. at the end of the maturing process, the values reached ph 5.55 +/- 0.03, 21.33 +/- 5.82%, and 44.06 +/- 12.47 mg/ 100 g, respectively. in the three studied anchovy batches, the biogenic amines tyramine, histamine, putrescine, cadaverine, and agmati ... | 2005 | 21132979 |
| assessment of bacterial diversity in the cattle tick rhipicephalus (boophilus) microplus through tag-encoded pyrosequencing. | ticks are regarded as the most relevant vectors of disease-causing pathogens in domestic and wild animals. the cattle tick, rhipicephalus (boophilus) microplus, hinders livestock production in tropical and subtropical parts of the world where it is endemic. tick microbiomes remain largely unexplored. the objective of this study was to explore the r. microplus microbiome by applying the bacterial 16s tag-encoded flx-titanium amplicon pyrosequencing (btefap) technique to characterize its bacterial ... | 2011 | 21211038 |
| antimicrobial susceptibility of coagulase-negative staphylococci isolated from bovine milk samples. | the aim of this study was to examine whether antimicrobial resistance profiles of coagulase-negative staphylococcus (cns) species isolated from milk of dairy cows differed between bacterial species, and to compare results obtained by phenotypic and genotypic profiling of resistance to penicillin, oxacillin and macrolide-lincosamide (ml) antibiotics. of 170 cns isolates, 83 (48.8%) were phenotypically susceptible to all antimicrobial agents tested in minimum inhibitory concentration (mic) assays, ... | 2011 | 21333468 |
| staphylococcus agnetis sp. nov., a coagulase-variable species from bovine subclinical and mild clinical mastitis. | thirteen gram-positive coagulase-variable staphylococci were isolated from subclinical and mild clinical mastitic bovine milk (n = 12) and a teat apex (n = 1). the results of sequence analysis of the 16s rrna gene and two housekeeping genes, rpob and tuf, and dna fingerprinting with amplified fragment length polymorphism (aflp) analysis show that the isolates form a separate branch within the genus staphylococcus. the phylogenetically most closely related species are s. hyicus and s. chromogenes ... | 2011 | 21335502 |
| the importance of staphylococci and threshold value of somatic cell count for diagnosis of sub-clinical mastitis in pirlak sheep at mid-lactation. | this study investigated the bacterial agents causing sub-clinical mastitis and the mean somatic cell counts (scc) of milk in pirlak sheep at mid-lactation. the percentage of infected udder halves was 11.4% (53/464). the most frequently isolated species were coagulase-negative staphylococci (cns) (64.2%), followed by staphylococcus aureus (24.5%) and escherichia coli (11.3%). among the cns, the most common species was staphylococcus epidermidis (38.2%). the other species isolated from milk sample ... | 2011 | 21366722 |
| some coagulase-negative staphylococcus species affect udder health more than others. | a longitudinal study in 3 dairy herds was conducted to profile the distribution of coagulase-negative staphylococcus (cns) species causing bovine intramammary infection (imi) using molecular identification and to gain more insight in the pathogenic potential of cns as a group and of the most prevalent species causing imi. monthly milk samples from 25 cows in each herd as well as samples from clinical mastitis were collected over a 13-mo period. coagulase-negative staphylococci were identified to ... | 2011 | 21524522 |
| vaccine development for the prevention of staphylococcal mastitis in dairy cows. | staphylococci are the most common and costly mammary disease of dairy cattle worldwide. target of rnaiii activating protein (trap), a membrane associated 167aa protein, is highly conserved among staphylococci and was shown in staphylococcus aureus to be involved in bacterial stress response. the aims of this study were to test the safety and efficacy of recombinant trap (rtrap) vaccine in dairy animals. the vaccine was safe as 2-3 subcutaneous injections of rtrap (54-100μg) with adjuvant isa 206 ... | 2011 | 21524801 |
| cns species and antimicrobial resistance in clinical and subclinical bovine mastitis. | coagulase-negative staphylococci (cns) are often associated with bovine mastitis. knowledge about the relative importance of specific cns species in different types of mastitis, and differences in antimicrobial resistance among cns species is, however, scarce. therefore, the aims of this study were to compare prevalence and antimicrobial susceptibility of cns species in clinical and subclinical mastitis using material from two national surveys. overall, staphylococcus chromogenes and staphylococ ... | 2011 | 21561725 |
| distribution of coagulase-negative staphylococcus species from milk and environment of dairy cows differs between herds. | in many parts of the world, coagulase-negative staphylococci (cns) are the predominant pathogens causing intramammary infections (imi) in dairy cows. the cows' environment is thought to be a possible source for cns mastitis and this was investigated in the present paper. a longitudinal field study was carried out in 6 well-managed dairy herds to determine the distribution and epidemiology of various cns species isolated from milk, causing imi and living freely in the cows' environment, respectiv ... | 2011 | 21605763 |
| [investigation of reduced vancomycin susceptibility in methicillin-resistant staphylococci.] | the first staphylococcus aureus strain with reduced susceptibility to vancomycin was reported from japan in 1996, and since then an increasing numbers of cases had been reported from various countries. along with the unfeasibility in the identification of these strains with routine laboratory methods, the use of glycopeptid antibiotics in infections due to these strains may result in therapeutic failure. the aim of this study was to investigate the prevalence of vancomycin intermediate staphyloc ... | 2011 | 21644067 |
| identification and antibiogram of microbes associated with bovine mastitis. | an investigation of mastitis in cattle was carried out in anand city and in nearby villages of gujarat state using california mastitis test (cmt) kit. the prevalence of clinical and subclinical mastitis was found to be 5.5% and 15.75%, respectively. staphylococcus aureus was identified through strain specific polymerase chain reaction; the remaining isolates identified on the basis of molecular analysis by 16s rdna sequencing and phylogenetic analysis were staphylococcus species, b. pumilus, sta ... | 2011 | 21774624 |
| molecular epidemiology of mastitis pathogens of dairy cattle and comparative relevance to humans. | mastitis, inflammation of the mammary gland, can be caused by a wide range of organisms, including gram-negative and gram-positive bacteria, mycoplasmas and algae. many microbial species that are common causes of bovine mastitis, such as escherichia coli, klebsiella pneumoniae, streptococcus agalactiae and staphylococcus aureus also occur as commensals or pathogens of humans whereas other causative species, such as streptococcus uberis, streptococcus dysgalactiae subsp. dysgalactiae or staphyloc ... | 2011 | 21968538 |
| Multiplex PCR assay for species identification of bovine mastitis pathogens. | To develop and evaluate a multiplex PCR (mPCR) assay for simultaneous detection of 10 bacterial species causing bovine mastitis namely, Staphylococcus aureus, Staphylococcus chromogenes, Staphylococcus epidermidis, Staphylococcus sciuri, Staphylococcus haemolyticus, Staphylococcus simulans, Streptococcus agalactiae, Streptococcus dysgalactiae, Streptococcus uberis and Escherichia coli in milk. | 2011 | 21972842 |
| Coagulase-negative staphylococci from non-mastitic bovine mammary gland: characterization of Staphylococcus chromogenes and Staphylococcus haemolyticus by antibiotic susceptibility testing and pulsed-field gel electrophoresis. | During routine microbiological examination of milk samples from dairy cows without clinical signs of mastitis, quarter milk samples of 231 dairy cows from 12 herds were investigated for the presence of coagulase-negative staphylococci (CNS). The isolates were identified on the basis of colony morphology, Gram staining, catalase and coagulase test and the commercial kit, API Staph. CNS was detected in 29% (67/231) of the cows. A total of seven CNS species were identified with the most prevalent b ... | 2011 | 22067091 |
| analysis of the bacterial diversity existing on animal hide and wool: development of a preliminary pcr-restriction fragment length polymorphism fingerprint database for identifying isolates. | twenty-one bacterial strains were isolated from imported cattle hide and rabbit wool using two types of media, nutrient broth, and nutrient broth with serum. the bacteria identified were brevibacillus laterosporus, leclercia adecarboxylata, peptococcus niger, bacillus circulans, raoultella ornithinolytica, bacillus subtilis, bacillus cereus, bacillus thermobacillus, bacillus choshinensis, bacillus sphaericus, acinetobacter haemolyticus, sphingomonas paucimobilis, bacillus thuringiensis, staphylo ... | 2012 | 23451394 |
| high-resolution melt analysis for species identification of coagulase-negative staphylococci derived from bovine milk. | coagulase-negative staphylococci (cns) are the most frequently isolated pathogens isolated from bovine milk. in this study, we report a rapid assay for species identification of cns using high-resolution melt analysis (hrma) of 16s rdna sequences. real-time polymerase chain reaction amplification of 16s rrna gene fragment, spanning the variable region v1 and v2, was performed with a resulting amplicon of 215 bp. a library of distinct melt curves of reference strains of 13 common cns species was ... | 2012 | 23273337 |
| potential use of bacillus thuringiensis bacteriocins to control antibiotic-resistant bacteria associated with mastitis in dairy goats. | mastitis caused by microbial infections in dairy goats reduces milk yield, modifies milk composition, and potentially contributes to morbidity in herds and consumers of dairy products. microorganisms associated with mastitis in dairy goats are commonly controlled with antibiotics, but it is known that continued use of these chemical agents promotes antibiotic resistance among bacterial populations. recently, it has been shown that bacteriocins of bacillus thuringiensis inhibit growth of food-bor ... | 2016 | 26022411 |
| comparative analysis of the acute response of zebrafish danio rerio skin to two different bacterial infections. | skin is an important innate immune organ in fish; however, little is known about the skin's immune response to infectious pathogens. we conducted a comparative analysis of the acute immune response of zebrafish danio rerio skin against gram-positive (staphylococcus chromogenes) and gram-negative (citrobacter freundii) bacterial infections. gene expression profiles induced from the two different infections were identified by microarray hybridization, with many genes demonstrating an acute immune ... | 2013 | 24341765 |
| distribution of cfr in staphylococcus spp. and escherichia coli strains from pig farms in china and characterization of a novel cfr-carrying f43:a-:b- plasmid. | the multi-resistance gene cfr is widely distributed among various gram-positive and gram-negative species in livestock in china. to better understand the epidemiology of cfr among staphylococcus spp. and e. coli isolates, 254 staphylococcus spp. and 398 e. coli strains collected from six swine farms in china were subjected to prevalence and genetic analysis. forty (15.7%) staphylococcus spp. isolates, including 38 staphylococcus sciuri strains, one staphylococcus chromogenes strain, and one stap ... | 2017 | 28293235 |
| visualizing the indefinable: three-dimensional complexity of 'infectious diseases'. | the words 'infection' and 'inflammation' lack specific definitions. here, such words are not defined. instead, the ability to visualize host-microbial interactions was explored. | 2015 | 25875169 |
| reduction of teat skin mastitis pathogen loads: differences between strains, dips, and contact times. | the purpose of these experiments was to (1) assess differences in mastitis pathogen strain sensitivities to teat disinfectants (teat dips), and (2) determine the optimum time for premilking teat dips to remain in contact with teat skin to reduce pathogen loads on teat skin. two experiments were conducted using the excised teat model. in experiment 1, the differences in mastitis pathogen strain sensitivities to 4 commercially available dips (dip a: 1% h2o2; dip b: 1% chlorine dioxide; dip c: 1% i ... | 2015 | 25497825 |
| antibacterial activity of essential oils, their blends and mixtures of their main constituents against some strains supporting livestock mastitis. | ten of the most known and used commercial essential oils (cinnamomum zeylanicum l., citrus bergamia risso, eucalyptus globulus labill., foeniculum vulgare mill., origanum majorana l., origanum vulgare l., rosmarinus officinalis l., satureja montana l., thymus vulgaris l. ct. carvacrol, thymus vulgaris l. ct. thymol) were tested against six bacteria strains staphylococcus aureus, staphylococcus chromogenes, staphylococcus sciuri, staphylococcus warneri, staphylococcus xylosus and escherichia coli ... | 2014 | 24727086 |
| short communication: prevalence of methicillin resistance in coagulase-negative staphylococci and staphylococcus aureus isolated from bulk milk on organic and conventional dairy farms in the united states. | the objective of this study was to evaluate the presence of methicillin-resistant staphylococcus aureus and coagulase-negative staphylococcus spp. in bulk tank milk samples from 288 organic and conventional dairy farms located in new york, wisconsin, and oregon from march 2009 to may 2011. due to recent publications reporting the presence mecc (a meca homolog not detected by traditional meca-based pcr methods), a combination of genotypic and phenotypic approaches was used to enhance the recovery ... | 2014 | 24582450 |
| effect of stage of lactation on the immune competence of goat mammary gland. | the present research was undertaken to evaluate the effect of stage of lactation on immune competence of the goat mammary gland. the prevalence and etiology of intramammary infections and cytokine pattern were studied in individual caprine milk during lactation. a total of 1,200 caprine milk samples were collected during early, mid, and late lactation (less than 40 d, from 110 to 130 d, and more than 200 d in lactation, respectively) and analyzed for milk quality, bacterial cell load, and cytoki ... | 2016 | 26947302 |
| bovine-associated cns species resist phagocytosis differently. | coagulase-negative staphylococci (cns) cause usually subclinical or mild clinical bovine mastitis, which often remains persistent. symptoms are usually mild, mostly only comprising slight changes in the appearance of milk and possibly slight swelling. however, clinical mastitis with severe signs has also been reported. the reasons for the differences in clinical expression are largely unknown. macrophages play an important role in the innate immunity of the udder. this study examined phagocytosi ... | 2013 | 24207012 |
| the diversities of staphylococcal species, virulence and antibiotic resistance genes in the subclinical mastitis milk from a single chinese cow herd. | staphylococci are the leading pathogens of bovine mastitis which is difficult to control. however, the published data on the prevalence of staphylococcal species, virulence and antibiotic resistance genes in bovine mastitis from china are limited. in this study, 104 out of 209 subclinical mastitis milk samples from a single chinese dairy herd were cultured-positive for staphylococci (49.8%), which were further identified as coagulase-positive staphylococci (cps) or coagulase-negative staphylococ ... | 2015 | 26276706 |
| an investigation of resistance to β-lactam antimicrobials among staphylococci isolated from pigs with exudative epidermitis. | a high proportion of staphylococci isolated from pigs affected with exudative epidermitis were found to be resistant to β-lactam antimicrobials. the primary objective of this research was to investigate and characterize β-lactam resistance in staphylococcus hyicus, staphylococcus aureus and other staphylococci isolated from these pigs. | 2013 | 24131819 |
| staphylococcus chromogenes, a coagulase-negative staphylococcus species that can clot plasma. | staphylococcus chromogenes is one of the main coagulase-negative staphylococci isolated from mastitis of dairy cows. we describe s. chromogenes isolates that can clot plasma. since the main pathogen causing mastitis is coagulase-positive staphylococcus aureus, the coagulase-positive phenotype of s. chromogenes described here can easily lead to misidentification. | 2016 | 26912749 |
| interaction between bovine-associated coagulase-negative staphylococci species and strains and bovine mammary epithelial cells reflects differences in ecology and epidemiological behavior. | bacteria adherence seems to be an essential first stage for the internalization of bacteria into the cytoplasm of the host cell, which is considered an important virulence strategy enabling bacteria to occupy a microenvironment separated from host defense mechanisms. thus, this study aimed to explore the difference in the capacity of 4 bovine-associated staphylococci species or strains to adhere to and internalize into bovine mammary epithelial cells (mec). three different isolates of coagulase- ... | 2016 | 26830736 |
| phenotypic antimicrobial susceptibility and occurrence of selected resistance genes in gram-positive mastitis pathogens isolated from wisconsin dairy cows. | in the united states, few intramammary antimicrobials exist that are approved for treatment of bovine mastitis; thus, ensuring judicious use of these products is a priority. the objectives of this study were to determine phenotypic susceptibility and presence of selected antimicrobial resistance genes from staphylococci, streptococci, and streptococcal-like organisms recovered from cases of clinical mastitis occurring in cows on large wisconsin farms. staphylococcus aureus (n=35 from 19 herds), ... | 2015 | 25912858 |
| species-level identification of staphylococci isolated from bovine mastitis in brazil using partial 16s rrna sequencing. | staphylococci isolated from bovine milk and not classified as staphylococcus aureus represent a heterogeneous group of microorganisms that are frequently associated with bovine mastitis. the identification of these microorganisms is important, although it is difficult and relatively costly. genotypic methods add precision in the identification of staphylococcus species. in the present study, partial 16s rrna sequencing was used for the species identification of coagulase-positive and coagulase-n ... | 2015 | 25704228 |
| technical note: a pilot study using a mouse mastitis model to study differences between bovine associated coagulase-negative staphylococci. | coagulase-negative staphylococci (cns) are a group of bacteria classified as either minor mastitis pathogens or commensal microbiota. recent research suggests species- and even strain-related epidemiological and genetic differences within the large cns group. the current pilot study investigated in 2 experiments whether a mouse mastitis model validated for bovine staphylococcus aureus can be used to explore further differences between cns species and strains. in a first dose titration experiment ... | 2015 | 25497801 |