| characterization of the streptococcus adjacens group antigen structure. | serological classification of bacteria requires the presence of an antigen unique to the organism of interest. streptococci are serologically differentiated by group antigens, many of which are carbohydrates, although some are amphiphiles. this report describes the chemical characterization of the streptococcus adjacens group antigen structure. previous studies demonstrated that the amphiphile contained phosphorus, ribitol, galactose, galactosamine, alanine, and fatty acids. phosphodiester bonds ... | 1992 | 1309524 |
| efficacy of temafloxacin in experimental streptococcus adjacens endocarditis and autoradiographic diffusion pattern of [14c]temafloxacin in cardiac vegetations. | temafloxacin, a new fluoroquinolone, alone or in combination with tobramycin, was compared with penicillin, tobramycin, and their combination in the therapy of rabbits with endocarditis caused by streptococcus adjacens gadt, a new species of nutritionally variant streptococci. animals were injected intramuscularly for 4 days with temafloxacin (50 mg/kg of body weight twice daily [b.i.d.]) alone or combined with tobramycin (12 mg/kg once daily), with procaine penicillin (150,000 u/kg b.i.d.) alon ... | 1992 | 1332590 |
| optimal aminoglycoside dosing regimen for penicillin-tobramycin synergism in experimental streptococcus adjacens endocarditis. | the combination of penicillin and aminoglycoside is the recommended therapy for endocarditis caused by nutritionally variant streptococci (nvs). however, the optimal aminoglycoside dosing regimen remains controversial. we compared the efficacies of four regimens of tobramycin alone or combined with procaine penicillin in the therapy of rabbits with endocarditis caused by streptococcus adjacens, a new species of nvs. animals were injected intramuscularly for 4 days with procaine penicillin (150,0 ... | 1992 | 1489184 |
| intraspecies variations in nutritionally variant streptococci: rrna gene restriction patterns of streptococcus defectivus and streptococcus adjacens. | the rrna gene restriction patterns of two species of nutritionally variant streptococci, streptococcus defectivus and streptococcus adjacens, were determined, and the results were compared with the electrophoretic migration profiles of penicillin-binding proteins. reference strains belonging to various streptococcal species were used as controls. our results correlated with the results of dna-dna hybridization experiments and confirmed the delineation of these two species. moreover, they demonst ... | 1991 | 1720653 |
| purification and characterization of streptococcus adjacens (nutritionally variant streptococcus serotype ii) group antigen. | nutritionally variant streptococci (nvs) possess amphiphiles which are serologically distinct from lipoteichoic acid and which serve as group-specific antigens for nvs. the objective of this study was to purify and characterize the nvs serotype ii (streptococcus adjacens) amphiphile. amphiphile was isolated from stationary-phase culture supernatants of nvs strain 81 (nvs serotype ii). phenol-water extracts of culture supernatants were subjected to hydrophobic interaction chromatography and gel f ... | 1991 | 1987076 |
| analysis of adherence of streptococcus defectivus and endocarditis-associated streptococci to extracellular matrix. | pathogenesis of nutritionally variant streptococcal (nvs) endocarditis initiates with bacterial attachment to and colonization of the damaged heart valve surface. underlying extracellular matrix (ecm) exposed to the environment during damage to cardiac endothelium provides additional receptors that could be involved in bacterial adherence. the ability of nvs and endocarditis-associated streptococci to bind ecm was investigated by using an enzyme-linked immunosorbent assay system that incorporate ... | 1991 | 1997435 |
| transfer of streptococcus adjacens and streptococcus defectivus to abiotrophia gen. nov. as abiotrophia adiacens comb. nov. and abiotrophia defectiva comb. nov., respectively. | we performed this study to determine the 16s rrna sequences of the type strains of streptococcus adjacens and streptococcus defectivus and to calculate the phylogenetic distances between these two nutritionally variant streptococci (nvs) and other members of the genus streptococcus. s. adjacens and s. defectivus belonged to one cluster, but this cluster was not closely related to other streptococcal species. a comparative analysis of the sequences of these organisms and other low-g+c-content gra ... | 1995 | 7547302 |
| native valve endocarditis due to nutritionally variant streptococcus adjacens associated with enterococcus faecium. | | 1995 | 7588853 |
| analysis of enzymatic activities for differentiation of two species of nutritionally variant streptococci, streptococcus defectivus and streptococcus adjacens. | strains of nutritionally variant streptococci, streptococcus defectivus (n = 10) and streptococcus adjacens (n = 20), were studied for the production of glycosidic and proteolytic enzyme activities. s. defectivus strains produced neuraminidase and alpha-fucosidase, while s. adjacens strains produced only neuraminidase. the s. adjacens strains produced a very wide range of proteolytic activities with the ability to hydrolyze the majority of aminopeptidase substrates tested, while s. defectivus st ... | 1995 | 7650191 |
| human endocarditis due to nutritionally variant streptococci: streptococcus adjacens and streptococcus defectivus. | streptococcus adjacens and s. defectivus are two recently individualized species of viridans streptococci. both species were previously designated as nutritionally variant streptococci and characterized by their growth as small satellite colonies supported by 'helper' bacteria, or requiring complex media enriched with cysteine or with pyridoxal. structural abnormalities were observed under these conditions and balanced growth can be obtained only with a semi-chemically defined medium, in which t ... | 1995 | 7671920 |
| [extravascular infections caused by streptococcus adjacens. report of 2 cases]. | | 1994 | 7811776 |
| influence of the pre-treatment duration of infection on the efficacies of various antibiotic regimens in experimental streptococcal endocarditis. | the influence of the pre-treatment duration of infection on the efficacies of three different antibiotic regimens was investigated in a rabbit model of subacute endocarditis caused by a novel, nutritionally-variant species, streptococcus adjacens strain gadt. treatment was initiated either 6 or 10 days after bacterial inoculation (days 7 and 11 respectively) and comprised procaine penicillin (150,000 iu/kg bd), alone or combined with tobramycin (12 mg/kg od), teicoplanin (10 mg/kg bd), all admin ... | 1993 | 8144424 |
| streptococcus adjacens endocarditis in a patient with nephrotic syndrome. | streptococcus adjacens has never previously been reported as an etiologic organism of infective endocarditis in taiwan. we describe a case of severe native valve endocarditis caused by s. adjacens, involving the mitral valve, the aortic valve, and the left atrium, in a 29-year-old woman with nephrotic syndrome on steroid therapy. blood cultures yielded gram-positive cocci that grew poorly on blood agar but strongly on chocolate agar. despite aggressive antibiotic treatment, the patients continue ... | 1997 | 9308330 |
| identification of abiotrophia adiacens and abiotrophia defectiva by 16s rrna gene pcr and restriction fragment length polymorphism analysis. | abiotrophia adiacens and abiotrophia defectiva, previously referred to as nutritionally variant streptococci, streptococcus adjacens and streptococcus defectivus, respectively, are causes of infective endocarditis. we describe a method of identifying these two species and also of distinguishing them from 15 other major etiological pathogens of infective endocarditis by means of 16s rrna gene pcr amplification followed by restriction fragment length polymorphism analysis (pcr-rflp). the 16s rrna ... | 1997 | 9316889 |
| growth differences of capnocytophaga canimorsus strains and some other fastidious organisms on various columbia-based blood agar media. | three human isolates of capnocytophaga canimorsus from blood cultures showed no growth when cultivated on sheep blood agar plates based on bbl columbia agar base, whereas the same three strains grew excellently on sheep blood agar plates based on difco columbia agar bases no. 2 or eh. two of the three strains also grew well on blood agar plates based on biomérieux columbia agar. strains that grew better on either difco or biomérieux columbia based blood agar plates were found among eikenella cor ... | 1995 | 9810657 |
| vertebral osteomyelitis and discitis associated with abiotrophia adiacens (nutritionally variant streptococcus) infection. | | 1998 | 9847960 |
| isolation of abiotrophia adiacens from a brain abscess which developed in a patient after neurosurgery. | we report the case of a patient who developed a large brain abscess after neurosurgery. cerebrospinal fluid from the abscess drainage yielded abiotrophia adiacens-specific pcr products and microorganisms that were identified by conventional microbiological methods and by 16s ribosomal dna analysis as abiotrophia adiacens, which was formerly classified as a member of nutritionally variant streptococci. | 1999 | 9986849 |
| abiotrophia species as a cause of endophthalmitis following cataract extraction. | microorganisms of the genus abiotrophia, members of the oral flora, are known as important causes of bacterial endocarditis. in this study, we report two individual cases of acute vitreous infection caused by abiotrophia adiacens and abiotrophia defectiva approximately a week after cataract extraction. abiotrophia isolates were recovered by cultivation of vitreous humor on chocolate agar and identified via conventional and api 20 strep identification systems. an 83-year-old male patient (a) and ... | 1999 | 10203522 |
| abiotrophia balaenopterae sp. nov., isolated from the minke whale (balaenoptera acutorostrata). | phenotypic and phylogenetic studies were performed on a hitherto undescribed micro-organism isolated from a minke whale (balaenoptera acutorostrata). comparative 16s rrna gene sequencing studies demonstrated that the unknown strain constituted a new subline close to, but distinct from, abiotrophia adiacens and abiotrophia elegans. the unknown bacterium was readily distinguished from these two abiotrophia species by biochemical tests and electrophoretic analysis of whole-cell proteins. on the bas ... | 1999 | 10319470 |
| abiotrophia elegans strains comprise 8% of the nutritionally variant streptococci isolated from the human mouth. | ninety-one isolates of nutritionally variant streptococci (nvs) that were previously isolated from the human mouth were regarded as consisting of 7 streptococcus defectivus isolates, 78 streptococcus adjacens isolates, and 6 gemella morbillorum isolates. however, recent references to the taxonomic reclassification of nvs, from s. defectivus to abiotrophia defectiva and from s. adjacens to abiotrophia adiacens, and the newly introduced species abiotrophia elegans as a third abiotrophia species, e ... | 1999 | 10405400 |
| endocarditis caused by abiotrophia species. | two cases of endocarditis with nutritionally variant streptococci are presented. such strains have recently been included in the new genus abiotrophia. a total of 12 additional abiotrophia strains, including the type strains of abiotrophia defectiva and abiotrophia adiacens, were characterized in order to comment on their microbiological characteristics. | 1999 | 10447338 |
| [abiotrophia related endocarditis: contribution of molecular biology]. | former nutritionally-deficient (variant) streptococci were recently separated from other streptococci viridans to constitute a new abiotrophia genus, subdivided into two species, abiotrophia defectiva (ex-streptococcus defectivus) and abiotrophia adjacens (ex-streptococcus adjacens). | 1999 | 10629690 |
| genetic heterogeneities and phenotypic characteristics of strains of the genus abiotrophia and proposal of abiotrophia para-adiacens sp. nov. | the genus abiotrophia represents a heterogeneous group of fastidious cocci that show a dependence on pyridoxal hydrochloride analogs for growth. the genetic heterogeneity in the genus abiotrophia was examined by dna-dna hybridization, pcr assay of genomic dna sequences, and restriction fragment length polymorphism and sequence homology analyses of the pcr-amplified 16s rrna gene. nine type or reference strains of abiotrophia defectiva, abiotrophia adiacens, and abiotrophia elegans and 36 oral ab ... | 2000 | 10655334 |
| the genus abiotrophia (kawamura et al.) is not monophyletic: proposal of granulicatella gen. nov., granulicatella adiacens comb. nov., granulicatella elegans comb. nov. and granulicatella balaenopterae comb. nov. | the genus abiotrophia currently includes four species, abiotrophia defectiva, abiotrophia adiacens, abiotrophia balaenopterae and abiotrophia elegans. recent 16s rrna gene sequencing studies have demonstrated that the genus is not monophyletic and is in need of taxonomic revision. phylogenetically, the genus abiotrophia consists of two distinct lines, a. defectiva, the type species of the genus, and a robust group consisting of a. adiacens, a. balaenopterae and a. elegans. therefore, it is forma ... | 2000 | 10826824 |
| antimicrobial susceptibility of abiotrophia adiacens and abiotrophia defectiva. | the susceptibilities of 27 abiotrophia adiacens (proposed reclassification granulicatella adiacens comb.nov., collins & lawson, 2000) and 12 abiotrophia defectiva isolates were tested by microdilution in pyridoxal hydrochloride and lysed horse blood supplemented mueller-hinton broth. according to nccls interpretative criteria for streptococcus spp. not streptococcus pneumoniae, the susceptibilities of a. adiacens and a. defectiva were, respectively: penicillin, 55% and 8%; amoxicillin, 81% and 9 ... | 2000 | 11109021 |
| abiotrophia species in early dental plaque. | the most characteristic traits that distinguish abiotrophia strains from other streptococci are nutrient requirements, satellitism and pyrrolidonyl arylamidase activity. the presence of abiotrophia strains was studied in early dental plaque formed on sucrose-containing and sucrose-free (glucose-containing) diets. the isolates were from 0- to 3-day dental plaque formed on the buccal surface of a lower premolar in six subjects. identification of abiotrophia strains was based on their pyrrolidonyl ... | 2000 | 11154413 |
| serological properties of abiotrophia and granulicatella species (nutritionally variant streptococci). | serological variations were examined among 12 type or reference strains and 91 oral isolates of vitamin b6-dependent abiotrophia and granulicatella spp. rabbits were immunized with whole cells of 12 selected strains and 10 typing antisera were obtained, which were unreactive with the lancefield group a to g antigen preparations. the reactivity of the antisera and autoclaved cell surface antigen extracts was tested by double diffusion in agar gel and a capillary precipitin test. these typing anti ... | 2000 | 11220686 |
| granulicatella and abiotrophia species from human clinical specimens. | one hundred one isolates of nutritionally variant streptococci from 97 patients were phenotypically characterized and compared with the type strains of granulicatella adiacens (formerly abiotrophia adiacens) (atcc 49175(t)) abiotrophia defectiva (atcc 49176(t)), and granulicatella elegans (formerly abiotrophia elegans) (dsm 11693(t)). of the isolates, 55 and 43 resembled g. adiacens and a. defectiva, respectively, while 3 strains resembled g. elegans. phenotypic characteristics useful in differe ... | 2001 | 11574566 |
| vertebral osteomyelitis and endocarditis of a pacemaker lead due to granulicatella (abiotrophia) adiacens. | systemic infection due to granulicatella (formerly abiotrophia), a species of nutrition-deficient gram-positive cocci, is rare. we present the case of a 68-year-old diabetic male who presented with back pain and a history of fever and chills. imaging studies revealed vertebral osteomyelitis of the th 10/11 region. transesophageal echocardiography disclosed a vegetation adjacent to the pacemaker lead and blood cultures grew granulicatella adiacens. a diagnosis of vertebral osteomyelitis and endoc ... | 2002 | 12382095 |
| granulicatella adiacens and abiotrophia defectiva bacteraemia characterized by 16s rrna gene sequencing. | traditionally, the identification, epidemiology and spectrum of clinical diseases caused by granulicatella adiacens and abiotrophia defectiva are dependent upon their phenotypic characterization. during a 6-year period (july 1995-june 2001), seven and two alpha-haemolytic streptococci were identified as g. adiacens and a. defectiva, respectively, by 16s rrna gene sequencing. three patients with haematological malignancies and neutropenic fever had primary bacteraemia. three patients with valvula ... | 2003 | 12543919 |
| septic arthritis caused by granulicatella adiacens: diagnosis by inoculation of synovial fluid into blood culture bottles. | granulicatella species, formerly known as nutritionally variant streptococci, cause a variety of infections, primarily endocarditis. we report the first culture-proven case of a granulicatella species causing septic arthritis. a 68-year-old female presented with knee pain and swelling. she was initially evaluated with arthrocentesis and arthroscopy, but no organism was identified. her pain improved after a brief course of antibiotics but recurred 3 months later. she underwent repeat arthrocentes ... | 2003 | 12720043 |
| usefulness of the microseq 500 16s ribosomal dna-based bacterial identification system for identification of clinically significant bacterial isolates with ambiguous biochemical profiles. | due to the inadequate automation in the amplification and sequencing procedures, the use of 16s rrna gene sequence-based methods in clinical microbiology laboratories is largely limited to identification of strains that are difficult to identify by phenotypic methods. in this study, using conventional full-sequence 16s rrna gene sequencing as the "gold standard," we evaluated the usefulness of the microseq 500 16s ribosomal dna (rdna)-based bacterial identification system, which involves amplifi ... | 2003 | 12734240 |
| a case of endocarditis due to granulicatella adiacens. | | 2003 | 12848740 |
| inhibition of fibronectin binding of some bacterial cells by subtle ph increase within the physiological range. | the fibronectin (fn)-binding ability of microorganisms is considered to be involved in their pathogenicities. granulicatella adiacens, a member of the oral flora and a causative agent of culture-negative infective endocarditis, showed nearly maximum binding to immobilized fn at ph 7.2 but greatly reduced binding at a slightly higher ph 7.4 and almost no binding at ph 7.6 in the presence of physiological concentration of nacl (0.15 m). a similar ph-sensitive fn-binding property was noted with esc ... | 2003 | 14499992 |
| "streptococcus milleri" endocarditis caused by streptococcus anginosus. | unlike other viridans streptococci, members of the "streptococcus milleri group" are often associated with abscess formation, but are only rare causes of infective endocarditis. although it has been shown that almost all s. intermedius isolates and most s. constellatus isolates, but only 19% of s. anginosus isolates, were associated with abscess formation, no report has addressed the relative importance of the 3 species of the "s. milleri group" in infective endocarditis. during a 5-year period ... | 2004 | 14972375 |
| identification and characterization of bacterial-binding property in the type iii repeat domain of fibronectin. | to characterize fibronectin binding with granulicatella adiacens, a causative agent of infective endocarditis, monoclonal antibodies were generated against human fibronectin and selected for their capacity to inhibit the fibronectin binding of the organism. thermolysin and lysyl-endopeptidase digests of fibronectin were characterized by western blot. the epitope of inhibitory monoclonal antibody was found in the central portion of fibronectin known as the cell-binding domain, and not in the n-te ... | 2004 | 15219833 |
| phenotypic and molecular characterization of erythromycin resistance in four isolates of streptococcus-like gram-positive cocci causing bacteremia. | among nine patients with bacteremia caused by granulicatella or gemella in a 6-year period (july 1995 to june 2001), three had bacteremia caused by erythromycin-resistant granulicatella adiacens and one had bacteremia caused by erythromycin-resistant gemella haemolysans. all four isolates possessed mef genes, whereas none possessed ermt, ermtr, or ermb genes. | 2004 | 15243100 |
| granulicatella (abiotrophia) adiacens infection associated with a total knee arthroplasty. | we describe the first case of prosthetic joint associated infection due to granulicatella adiacens (formerly abiotrophia adiacens). diagnosis was made by broad spectrum pcr, and later by culture. diagnosis and treatment of this microorganism is difficult. two y after revision and antibiotic treatment, infection was under control but not cured. | 2004 | 15513405 |
| detection of novel oral species and phylotypes in symptomatic endodontic infections including abscesses. | this study was undertaken to investigate the occurrence of several uncultivated phylotypes and newly named bacterial species in symptomatic endodontic infections. samples taken from cases clinically diagnosed as acute periradicular abscesses or acute periradicular periodontitis were surveyed for the presence of 12 taxa by means of a 16s rrna-gene-based nested or hemi-nested pcr assay. the most prevalent of the target taxa were dialister invisus, olsenella uli, granulicatella adiacens, and synerg ... | 2005 | 16099112 |
| prosthetic valve endocarditis from granulicatella adiacens (nutritionally variant streptococci). | nutritionally variant streptococci (nvs) are an unusual but significant cause of endocarditis, with increased morbidity and mortality compared to those caused by enterococci or viridans streptococci. for many reasons, nvs endocarditis has remained very difficult to treat medically with antibiotics, and when the bacteria infect prosthetic valves, surgical intervention has historically been required to effect cure. we report the first case of granulicatella adiacens prosthetic valve endocarditis ( ... | 2004 | 16230190 |
| streptococci dominate the diverse flora within buccal cells. | previously, we reported that intracellular actinobacillus actinomycetemcomitans, porphyromonas gingivalis, and tannerella forsythensis were present within buccal epithelial cells from human subjects, as lesser components of a polymicrobial flora. in this study, we further characterized that intracellular flora by using the same double-labeling techniques to identify fusobacterium nucleatum, prevotella intermedia, oral campylobacter species, eikenella corrodens, treponema denticola, gemella haemo ... | 2005 | 16304448 |
| bloodstream and endovascular infections due to abiotrophia defectiva and granulicatella species. | abiotrophia and granulicatella species, previously referred to as nutritionally variant streptococci (nvs), are significant causative agents of endocarditis and bacteraemia. in this study, we reviewed the clinical manifestations of infections due to a. defectiva and granulicatella species that occurred at our institution between 1998 and 2004. | 2006 | 16426445 |
| catonella morbi and granulicatella adiacens: new species in endodontic infections. | this study intended to examine samples from primary endodontic infections for the presence of catonella morbi and granulicatella adiacens, 2 species that have been recently suggested to be involved with infections in other oral sites. | 2006 | 16876072 |
| adherence of abiotrophia defectiva and granulicatella species to fibronectin: is there a link with endovascular infections? | during a 6-year period, we isolated three abiotrophia defectiva, six granulicatella adiacens and two g. 'para-adiacens' strains from clinical specimens. all a. defectiva strains were isolated from immunocompetent patients with endovascular infections, whereas the granulicatella spp. strains were isolated from immunosuppressed patients with primary bacteremia. as the capacity of bacteria to adhere to the host extracellular matrix (ecm) has been implicated in the pathogenesis of endovascular infec ... | 2006 | 17064277 |
| [endocarditis due to granulicatella adiacens]. | case report: a prosthetic aortic valve was implanted in a 41-year-old patient with connatal aortic stenosis. 14 months later a bacterial endocarditis was diagnosed and treated with ceftriaxone. 6 weeks later he had a relapse. satellite streptococci could be cultivated from three blood cultures, later identified as granulicatella adiacens. the patient was treated with penicillin and gentamicin. review of the literature: in a short review of the literature the most important aspects of pathogenesi ... | 2007 | 17221353 |
| [endocarditis due to granulicatella adiacens in an old patient]. | | 2007 | 17565887 |
| characterization of the bacteriolytic activity of nutritionally variant streptococci. | the bacteriolytic activity of nutritionally variant streptococci (nvs), fastidious microaerophilic bacteria, which are members of the genera abiotrophia and granulicatella, was characterized in a renaturating sds polyacrylamide gel electrophoresis system. bacteriolytic profiles appeared quite different for the three species of nvs examined. granulicatella adiacens or abiotrophia defectiva each presented at least seven lytic bands, four of which were in common, while the other three were species- ... | 2007 | 17889570 |
| infectious intracranial aneurysms caused by granulicatella adiacens. | infectious intracranial aneurysms (iias) are uncommon but severe complications of bacterial endocarditis. we report a case of iia due to a rare nutritionally variant streptococcus, granulicatella adiacens. the 31-year-old young patient, without any known risk for infective endocarditis had a silent clinical course until rupture of iias occurred. despite antibiotic treatment and surgical clipping of iias, a second episode of more severe intracerebral hemorrhage occurred four months later. mitral ... | 2008 | 17928184 |
| infective endocarditis caused by nutritionally variant streptococci. | infective endocarditis caused by nutritionally variant streptococci (nvs) has a higher rate of complications than endocarditis caused by other streptococci. the bacteriologic failure rate and the mortality rate are high. however, current knowledge on this disease derives from previous patient data from 1987. recent case reports showed successful antibiotic treatment in the absence of surgery. here, we report the clinical outcome of infective endocarditis caused by nvs in our hospital. | 2007 | 18030177 |
| a novel agent of peritoneal dialysis-related peritonitis: granulicatella adiacens. | | 2008 | 18178954 |
| granulicatella adiacens breast implant-associated infection. | the 1st reported case of breast implant-associated infection due to granulicatella adiacens, formerly known as nutritionally variant streptococci, streptococcus adiacens, and abiotrophia adiacens is presented. microbiology and previously reported cases of infections by this organism are reviewed. | 2008 | 18206331 |
| helcococcus ovis, an emerging pathogen in bovine valvular endocarditis. | the initial isolation of helcococcus ovis from a valvular thrombus prompted us to investigate the prevalence of this bacterium in bovine valvular endocarditis. specimens from 55 affected hearts were examined by culture using columbia blood agar and cross streaking the inoculated plate with a staphylococcus aureus strain. as confirmed by 16s rrna gene sequencing, h. ovis was isolated with an unexpectedly high frequency of 33%, predominantly as heavy growth and pure culture. the majority of h. ovi ... | 2008 | 18716228 |
| fluorescence in situ hybridisation (fish) accelerates identification of gram-positive cocci in positive blood cultures. | sepsis is a life-threatening disease with a high mortality rate. rapid identification of blood culture isolates plays a crucial role in adequate antimicrobial therapy in sepsis patients. to accelerate microbiological diagnosis, a comprehensive panel of oligonucleotide probes for fluorescence in situ hybridisation (fish) targeting gram-positive cocci was compiled and evaluated on 428 positive blood culture specimens. by combining genus-specific and species-specific probes, the assay allowed discr ... | 2008 | 18718741 |
| analytical limits of three beta-glucosidase-based commercial culture methods used in environmental microbiology, to detect enterococci. | the enzyme-based test methods enterolert, chromocult enterococci agar, and mei agar, used to assess water quality through the detection enterococcus spp., have been compared in terms of their analytical specificity and their ability to detect various enterococcal strains. to achieve this goal, we have tested 110 different non-enterococcal bacterial strains and 101 strains of enterococcus spp. isolated from diverse origins. the results obtained showed that 69 (68.3%), 84 (83.2%), and 89 (88.1%) o ... | 2009 | 19700833 |
| comparisons of subgingival microbial profiles of refractory periodontitis, severe periodontitis, and periodontal health using the human oral microbe identification microarray. | this study compared the subgingival microbiota of subjects with refractory periodontitis (rp) to those in subjects with treatable periodontitis (grs = good responders) or periodontal health (ph) using the human oral microbe identification microarray (homim). | 2009 | 19722792 |
| abiotrophia adiacens-related infectious crystalline keratopathy following penetrating keratoplasty. | | 2010 | 20385536 |
| use of groesl as a target for identification of abiotrophia, granulicatella, and gemella species. | we determined the groesl sequences of three species of nutritionally variant streptococci (abiotrophia defectiva, granulicatella adiacens, and granulicatella elegans) and three gemella species (gemella morbillorum, gemella haemolysans, and gemella sanguinis). the nucleotide sequence similarities between the groes and groel genes of the above genera were 41.7 to 85.9% and 63.7 to 84.3%, respectively. the intraspecies similarities of groesl sequences for the isolates of abiotrophia and granulicate ... | 2010 | 20686088 |
| vertebral osteomyelitis associated with granulicatella adiacens. | granulicatella adiacens is a nutritionally variant streptococci. only 3 cases of vertebral osteomyelitis due to these microorganisms have been reported. we experienced a 73-year-old male who consulted us due to fever and back pain of about 1-month duration. on examination, a presystolic murmur was heard in the apical region. echocardiography showed prolapse of the mitral valve, but no vegetation was observed. mri revealed osteomyelitis of lumbar vertebrae. as g. adiacens was detected in blood cu ... | 2010 | 21319041 |
| native valve endocarditis and femoral embolism due to granulicatella adiacens: a rare case report. | the fastidious gram-positive cocci granulicatella adiacens, previously known as nutritionally variant streptococci (nvs) are unusual but significant cause of endocarditis due to increased mortality and morbidity. difficulties in reaching correct bacteriological identification, increased resistance to beta-lactam antibiotics and macrolides and complicated clinical course have contributed to problems in management of cases of infective endocarditis caused by this bacterium. we present the first in ... | 2010 | 21340307 |
| clinical and microbiological studies of children and adolescents receiving orthodontic treatment. | this case-controlled study examined clinical and microbiological parameters in brazilian children and adolescents receiving orthodontic treatment using fixed orthodontic appliances or removable orthodontic appliances. | 2010 | 21344829 |
| granulicatella adiacens bacteremia in an elderly trauma patient. | abstract background: granulicatella adiacens is a normal commensal of human mucosal surfaces, inhabiting the oral cavity and urogenital and gastrointestinal tracts and only rarely causing disease. these gram-positive cocci can be difficult to identify. case report: we report a first case of bacteremia caused by granulicatella adiacens in an elderly female trauma victim. the infection was diagnosed accurately, and the patient was treated successfully. conclusion: granulicatella adiacens may be a ... | 2011 | 21524203 |
| an unusual causative agent for an orbital abscess: granulicatella adiacens. | we present a patient who developed an orbital abscess secondary to a retained organic (wooden) foreign body after a fall. clinically, he had a right sub brow fistula exuding purulent discharge, four millimetres of proptosis, limitation of ocular motility in all directions of gaze, but no signs of optic neuropathy. preoperative computed tomography and magnetic resonance imaging of the orbits was performed to localise the abscess and to facilitate surgical planning. he underwent exploration, drain ... | 2011 | 21574808 |
| [granulicatella adiacens: an underestimated cause of endocarditis]. | we report the case of an old man who was affected by an endocarditis related to granulicatella adiacens, an uncommon bacteria, difficult to isolate and responsible for an important morbidity and mortality. thus, it is mandatory to seek for endocarditis when this germ is demonstrated in order to start quickly an effective antibiotic treatment. inversely, in the presence of unexplained endocarditis, further bacteriological investigations are needed to seek for this life nutrition deficient bacteri ... | 2011 | 21834448 |
| Granulicatella adiacens and early-onset sepsis in neonate. | | 2011 | 22000391 |
| the subgingival microbiota of papillon-lefèvre syndrome. | background: there is little information about the microbiological profiles of periodontal lesions in papillon-lefèvre syndrome (pls) and the significance of bacteria in the pathogenesis of periodontitis in these patients. this comprehensive analysis of the subgingival microbiota in pls patients used 16s rrna clonal analysis and the 16s rrna-based human oral microbe identification microarray (homim). methods: thirteen pls patients, from 7 unrelated families, volunteered for this microbiological s ... | 2011 | 22141356 |
| identification and characterization of a fibronectin-binding protein from granulicatella adiacens. | the interaction of microorganisms with fibronectin plays an important role in infective endocarditis. granulicatella adiacens is a member of the oral microbiota, formerly known as nutritionally variant streptococci, and is often isolated from endocarditis patients. in the present study we identified a surface protein, designated cha, which binds to fibronectin, by a plaque hybridization procedure using the csha sequence as probe, which encodes a fibronectin-binding molecule of streptococcus gord ... | 2011 | 22053963 |
| variations of oral microbiota are associated with pancreatic diseases including pancreatic cancer. | objectivethe associations between oral diseases and increased risk of pancreatic cancer have been reported in several prospective cohort studies. in this study, we measured variations of salivary microbiota and evaluated their potential associations with pancreatic cancer and chronic pancreatitis.methodsthis study was divided into three phases: (1) microbial profiling using the human oral microbe identification microarray to investigate salivary microbiota variation between 10 resectable patient ... | 2011 | 21994333 |
| granulicatella adiacens--an unusual causative agent for carbuncle. | | 2012 | 23455831 |
| microbial signature profiles of periodontally healthy and diseased patients. | to determine microbial profiles that discriminate periodontal health from different forms of periodontal diseases. | 2014 | 25139407 |
| coaggregation and biofilm growth of granulicatella spp. with fusobacterium nucleatum and aggregatibacter actinomycetemcomitans. | members of fastidious granulicatella and aggregatibacter genera belong to normal oral flora bacteria that can cause serious infections, such as infective endocarditis. aggregatibacter actinomycetemcomitans has long been implicated in aggressive periodontitis, whereas dna-based methods only recently showed an association between granulicatella spp. and dental diseases. as bacterial coaggregation is a key phenomenon in the development of oral and nonoral multispecies bacterial communities it would ... | 2015 | 26025449 |
| oral microbiota shift after 12-week supplementation with lactobacillus reuteri dsm 17938 and pta 5289; a randomized control trial. | lactobacillus spp. potentially contribute to health by modulating bacterial biofilm formation, but their effects on the overall oral microbiota remain unclear. | 2015 | 25946126 |
| comparison of oral microbiota in tumor and non-tumor tissues of patients with oral squamous cell carcinoma. | bacterial infections have been linked to malignancies due to their ability to induce chronic inflammation. we investigated the association of oral bacteria in oral squamous cell carcinoma (oscc/tumor) tissues and compared with adjacent non-tumor mucosa sampled 5 cm distant from the same patient (n = 10). by using culture-independent 16s rrna approaches, denaturing gradient gel electrophoresis (dgge) and cloning and sequencing, we assessed the total bacterial diversity in these clinical samples. | 2012 | 22817758 |
| characterization of the salivary microbiome in patients with pancreatic cancer. | clinical manifestations of pancreatic cancer often do not occur until the cancer has undergone metastasis, resulting in a very low survival rate. in this study, we investigated whether salivary bacterial profiles might provide useful biomarkers for early detection of pancreatic cancer. using high-throughput sequencing of bacterial small subunit ribosomal rna (16s rrna) gene, we characterized the salivary microbiota of patients with pancreatic cancer and compared them to healthy patients and pati ... | 2015 | 26587342 |
| differential utilization of basic proline-rich glycoproteins during growth of oral bacteria in saliva. | although saliva is widely recognized as a primary source of carbon and nitrogen for growth of the dental plaque biofilm community, little is known about how different oral bacteria utilize specific salivary components. to address this question, 32 strains representing 16 genera commonly isolated from early plaque biofilms were compared for growth over two transfers in stimulated (by chewing parafilm) whole saliva that was stabilized by heat treatment and dialysis. the cell densities, measured by ... | 2016 | 27316966 |
| erratum for alberti et al., antimicrobial susceptibilities of abiotrophia defectiva, granulicatella adiacens, and granulicatella elegans. | | 2016 | 27217443 |
| differential antimicrobial susceptibilities of granulicatella adiacens and abiotrophia defectiva. | mics of 25 abiotrophia defectiva and 109 granulicatella adiacens isolates were determined by broth microdilution. using clsi breakpoints, the susceptibilities of a. defectiva and g. adiacens isolates were, respectively, 24% and 34% to penicillin, 92% and 22% to ceftriaxone, 48% and 3% to cefepime, 72% and 87% to meropenem, 92% and 10% to cefotaxime, 100% and 97% to levofloxacin, 92% and 80% to clindamycin, and 24% and 50% to erythromycin. all isolates were susceptible to vancomycin. in the penic ... | 2016 | 27216060 |
| antimicrobial susceptibilities of abiotrophia defectiva, granulicatella adiacens, and granulicatella elegans. | nutritionally variant streptococci (nvs) are fastidious gram-positive cocci comprised of the species abiotrophia defectiva, granulicatella adiacens, and granulicatella elegans. nvs are an important cause of bacteremia and infective endocarditis (ie) associated with significant morbidity and mortality. antimicrobial susceptibility testing (ast) was performed for 14 antimicrobials using the broth microdilution mic method described in the clinical and laboratory standards institute (clsi) m45 guide ... | 2015 | 26666926 |
| comparison of maldi-tof ms and vitek 2 system for laboratory diagnosis of granulicatella and abiotrophia species causing invasive infections. | granulicatella and abiotrophia spp. were known as nutritionally variant streptococci (nvs). such strains have caused major diagnostic difficulties due to fastidious culturing and unspecific colony morphology. the present study is aimed at comparing the performance of laboratory available diagnostic methods for nvs isolates and determining the antimicrobial susceptibility of these isolates. fourteen clinical invasive isolates, consisting of 10 granulicatella adiacens, 1 granulicatella elegans, an ... | 2013 | 24034902 |
| microbiological diversity of peri-implantitis biofilm by sanger sequencing. | to examine the microbial diversity associated with implants with or without peri-implantitis and to identify differences between the bacterial communities in these clinical conditions. | 2014 | 23845046 |
| microbial transformation from normal oral microbiota to acute endodontic infections. | endodontic infections are a leading cause of oro-facial pain and tooth loss in western countries, and may lead to severe life-threatening infections. these infections are polymicrobial with high bacterial diversity. understanding the spatial transition of microbiota from normal oral cavities through the infected root canal to the acute periapical abscess can improve our knowledge of the pathogenesis of endodontic infections and lead to more effective treatment. we obtained samples from the oral ... | 2012 | 22839737 |
| granulicatella adiacens prosthetic hip joint infection after dental treatment. | granulicatella adiacens is a gram-positive bacteria and a normal component of oral flora. it is also found in dental plaques, endodontic abscesses and can rarely cause more serious infections. | 2016 | 28348763 |
| infections related to granulicatella adiacens: report of two cases and review of literature. | infections due to nutritionally variant streptococci are diagnosed rarely due to difficulties encountered during identification and isolation. mortality rate in these infections is high therefore appropriate supplemented media and reliable detection systems should be implemented to isolate these fastidious organisms. here, we describe two cases of granulicatella adiacens infections. all microbiologic identifications were made with maldi-tof vitek ms (biomerieux, france), and the results confirme ... | 2017 | 27934842 |
| infective endocarditis and sjögren's syndrome diagnosed simultaneously. | poor dentition and/or dental infection due to insufficient oral care are presumed to be risk factors for infective endocarditis (ie). we present a case of endocarditis caused by granulicatella adiacens and sjögren's syndrome (ss) with oral complications diagnosed simultaneously. a 67-year-old woman was admitted to our hospital with fever, general fatigue, arthralgia, and back pain. she was diagnosed with primary ss according to the criteria of the american-european consensus group. transthoracic ... | 2017 | 27920981 |
| vertebral osteomyelitis due to granulicatella adiacens, a nutritionally variant streptococci. | vertebral osteomyelitis is a common pathology affecting the spine. we present the case of a 46-year-old male who was diagnosed with progressive l2 vertebral osteomyelitis due to a rare pathogen, granulicatella adiacens. granulicatella adiacens is part of the normal body flora and is often difficult to culture on traditional mediums. the patient required a lateral corpectomy and posterior fixation for spinal stabilization and source control. | 2016 | 27800289 |
| granulicatella adiacens : an unusual isolate from urethral discharge. | | 2017 | 27514980 |
| case report of granulicatella adiacens as a cause of bacterascites. | granulicatella adiacens is a gram-positive coccus, formerly grouped with nutritionally variant streptococcus, often found as commensal bacteria of the human oral cavity, urogenital tract, and gastrointestinal tract. prior case reports have demonstrated granulicatella spp. as a pathogen that can cause bacteremia and infective endocarditis particularly of prosthetic valves and pacemaker leads. here, we report on a unique case of granulicatella adiacens bacterascites in a 50-year-old male. | 2015 | 26618012 |
| granulicatella adiacens bacteremia after flexible sigmoidoscopy. | | 2016 | 26323512 |
| granulicatella adiacens, an unusual causative agent in chronic dacryocystitis. | granulicatella adiacens, a recent taxonomic addition, is a commensal organism of the oral, gastrointestinal, and urogenital tracts and is rarely encountered in the orbit and eye. | 2015 | 25897344 |
| salivary microbiota associated with immunoglobulin a nephropathy. | this study aimed at investigating the salivary microbiota of 28 patients affected by immunoglobulin a nephropathy (igan). fourteen healthy volunteers (hc) were used as control. compared to hc, the number of some cultivable bacteria groups (e.g., total anaerobes) significantly (p < 0.05) decreased in the salivary samples of igan patients. total bacteria from salivary samples of igan patients and hc subjects were analyzed by pyrosequencing of 16s rrna gene. paired t test showed no significant (p > ... | 2015 | 25763757 |
| granulicatella adiacens subacute bacterial endocarditis as the underlying cause of type ii mixed cryoglobulinaemia. | a 57-year-old man with type ii mixed cryoglobulinaemia presented to the emergency department with a history of worsening lethargy, malaise and non-drenching night sweats in a relapsing-remitting pattern. he was diagnosed with type ii mixed cryoglobulinaemia 7 months ago following episodes of fever, night sweats, lethargy and malaise associated with a non-blanching, purpuric, raised erythematous rash that responded partially to immunosuppressive therapy and short courses of oral antibiotics. a si ... | 2015 | 25568266 |
| infective endocarditis due to granulicatella adiacens: a case report and review. | infective endocarditis (ie) caused by nutritionally variant streptococci (nvs) is associated with high bacteriologic and treatment failure and mortality rates compared to endocarditis caused by other streptococci. with automated blood culture systems, the rates of nvs-associated ie accounts for 5%-6% cases. we report a case of ie caused by nvs in an elderly female patient with no risk factors. the patient was successfully treated with combination antimicrobial therapy. | 2014 | 24727523 |
| discrepant satellitism for identification of granulicatella adiacens isolates. | | 2014 | 24624359 |
| pyogenic discitis due to abiotrophia adiacens. | abiotrophia species have been referred to as nutritionally variant streptococci because of their fastidious nutritional requirements for growth. abiotrophia species are difficult to identify with conventional solid culture. | 2013 | 24240080 |
| bivalvular endocarditis due to granulicatella adiacens. | male, 50 final diagnosis: bivalvular endocarditis due to granulicatella adiacens symptoms: fever • fatigue | 2013 | 24175010 |
| infective endocarditis caused by granulicatella adiacens. | granulicatella adiacens, a recently nomenclatured bacterium, was considered as one of the nutritionally variant streptococci (nvs) and is a mouth commensal. it is redesignated as a streptococcus like bacterium since it differs from streptococci. we report a case of infective endocarditis (ie) caused by this fastidious and unusual bacteria in a 63-year-old man with rheumatic valvular heart disease. g. adiacens was isolated from four of his blood culture samples, which was sensitive to beta lactam ... | 2015 | 23993006 |
| prosthetic knee arthritis due to granulicatella adiacens after dental treatment. | we report prosthetic knee arthritis in a 55-year-old diabetic man due to granulicatella adiacens, a micro-organism present in the oral flora, usually described in endocarditis but rarely in prosthesis joint infection. this patient had undergone a dental extraction without antibiotic prophylaxis one month before, and an aseptic loosening of the prosthesis had been diagnosed previously. if antimicrobial prophylaxis against infective endocarditis for dental procedures is well established, such an a ... | 2013 | 23764743 |
| pasteurella canis and granulicatella adiacens conjunctivitis outbreak resistant to empirical treatment in a child welfare agency. | to investigate pathogens associated with bacterial conjunctivitis resistant to the empirical treatment and their antibiotic resistance in a child welfare agency. | 2013 | 22800794 |
| endocarditis caused by nutritionally variant streptococci: a case report and literature review. | infective endocarditis (ie) due to abiotrophia and granulicatella species, previously referred to as nutritionally variant streptococci (nvs), occurs rarely and is often associated with negative blood cultures. rates of treatment failure, infection relapse and mortality are higher than those of endocarditis caused by other viridans streptococci. we report a case of endocarditis caused by granulicatella adiacens in a young man with no risk factors, who was successfully treated with surgery and co ... | 2012 | 22767303 |