| nucleotide sequence and taxonomy of cycas necrotic stunt virus. brief report. | cycas necrotic stunt virus (cnsv) is the only well-characterized virus from gymnosperm. cdna segments corresponding to the bipartite genome rnas (rna1, rna2) were synthesized and sequenced. each rna encoded a single polyprotein, flanked by the 5' and 3' non-coding regions (ncr) and followed by a poly (a) tail. the putative polyproteins encoded by rna1 and rna2 had sets of motifs, which were characteristic of viruses in the genus nepovirus. the polyproteins showed higher sequence identities to ar ... | 2002 | 12417954 |
| development of degenerate and species-specific primers for the differential and simultaneous rt-pcr detection of grapevine-infecting nepoviruses of subgroups a, b and c. | based on the nucleotide sequence homology of rna-1 and rna-2 of nepoviruses isolated from grapevines, three sets of degenerate primers, one for each of the three subgroups of the genus (a, b and c), were designed and proved effective for rt-pcr detection of subgroups in infected grapevines and herbaceous hosts. primers designed specifically for detecting subgroup a species amplified a fragment of 255 bp from samples infected by grapevine fanleaf virus (gflv), arabis mosaic virus (armv), tobacco ... | 2007 | 17187868 |
| next generation sequencing and molecular analysis of artichoke italian latent virus. | next-generation sequencing (ngs) allowed the assembly of the complete rna-1 and rna-2 sequences of a grapevine isolate of artichoke italian latent virus (ailv). rna-1 and rna-2 are 7,338 and 4,630 nucleotides in length excluding the 3' terminal poly(a) tail, and encode two putative polyproteins of 255.8 kda (p1) and 149.6 kda (p2), respectively. all conserved motifs and predicted cleavage sites, typical for nepovirus polyproteins, were found in p1 and p2. ailv p1 and p2 share high amino acid ide ... | 2017 | 28247096 |
| infection cycle of artichoke italian latent virus in tobacco plants: meristem invasion and recovery from disease symptoms. | nepoviral infections induce recovery in fully expanded leaves but persist in shoot apical meristem (sam) by a largely unknown mechanism. the dynamics of infection of a grapevine isolate of artichoke italian latent virus (ailv-v, genus nepovirus) in tobacco plants, including colonization of sam, symptom induction and subsequent recovery of mature leaves from symptoms, were characterized. ailv-v moved from the inoculated leaves systemically and invaded sam in 7 days post-inoculation (dpi), remaini ... | 2014 | 24911029 |