Publications

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tylenchida associated with different crops in sennar state (sudan).a study was done on the taxonomy and morphology of plant parasitic nematodes (tylenchida) found in sennar state (sudan). sixty samples of different crops were collected in the sugarcane area. thirty samples originated from soil around the roots of saccharum officinarum (sugarcane) from different ratoons and thirty samples were collected from other crops (mangifera indica; citrus limon; citrus aurantifolia; citrus paradisi; citrus sinensis, phoenix dactylifera, musa sapentium; cassia italica, cap ...200112425088
survey of crop losses in response to phytoparasitic nematodes in the united states for 1994.previous reports of crop losses to plant-parasitic nematodes have relied on published results of survey data based on certain commodities, including tobacco, peanuts, cotton, and soybean. reports on crop-loss assessment by land-grant universities and many commodity groups generally are no longer available, with the exception of the university of georgia, the beltwide cotton conference, and selected groups concerned with soybean. the society of nematologists extension committee contacted extensio ...199919270925
host suitability of eight prunus spp. and one pyrus communis rootstocks to pratylenchus vulnus, p. neglectus, and p. thornei.the effects of pratylenchus vulnus on rootstocks of eight commonly used prunus spp. and one pyrus communis were evaluated under greenhouse conditions during a 15-month period. in a first experiment, two almonds (moncayo and garrigues), one peach (gf-305), and two peach-almond hybrids (gf-677 and adafuel) inoculated with 2,000 nematodes per plant proved to be good hosts of p. vulnus. highest (p < 0.05) numbers of nematodes per gram of fresh root weight were recovered from adafuel and gf-677. root ...199119283165
rather than by direct acquisition via lateral gene transfer, ghf5 cellulases were passed on from early pratylenchidae to root-knot and cyst nematodes.plant parasitic nematodes are unusual metazoans as they are equipped with genes that allow for symbiont-independent degradation of plant cell walls. among the cell wall-degrading enzymes, glycoside hydrolase family 5 (ghf5) cellulases are relatively well characterized, especially for high impact parasites such as root-knot and cyst nematodes. interestingly, ancestors of extant nematodes most likely acquired these ghf5 cellulases from a prokaryote donor by one or multiple lateral gene transfer ev ...201223171084
invasion of wheat roots by pratylenchus thornei. 19676061807
a pest management approach to the control of pratylenchus thornei on wheat in mexico.the lesion nematode, pratylenchus thornei, was clearly demonstrated as a parasite of wheat. it reduced plant stands and stunted plants in the field under the environmental conditions found in sonora, mexico. other soil organisms also may have contributed to the problem. the nematode is widely distributed throughout the wheat-growing region, and may be a problem each growing season. nematicides controlled the nematode and increased yields, but they were not economical. no resistance was found in ...197419308110
effect of time, temperature, and inoculum density on reproduction of pratylenchus thornei in carrot disk cultures.reproduction of pratylenchus thornei on carrot disk cultures at different time periods after inoculation, temperature, and initial inoculum density was studied. at 25 c and with an initial inoculum of 25 females per disk, the final nematode population increased with increasing time after inoculation, although the populations after 25 and 50 days were not different. nematode numbers increased by 1,255-fold and 3,619-fold at 75 and 100 days, respectively. over 35 days incubation at 15, 20, 25, and ...199519277270
yield loss caused by pratylenchus thornei on wheat in south australia.a two-year field trial with 130 plots was conducted at tanunda, south australia. ten cereal cultivars differing in susceptibility to pratylenchus thornei, two poor host crops (non-leguminous), and a bare fallow treatment were used to manipulate the numbers of nematodes in the plots in the first year. initial and final densities were determined for each plot and varied from 0 to 9,400 nematodes/200 g oven-dried soil at the beginning of the second year. a highly susceptible wheat cultivar, warigal ...199919270909
effect of field crops on density of pratylenchus in southeastern australia; part 2: p. thornei.the effect of 93 field crop and pasture cultivars on the end-of-season population densities of pratylenchus thornei in soil was determined in the field in southeastern australia. wheat and barley cultivars had different effects on the population densities of p. thornei. most commercial wheat cultivars that are grown in southeastern australia were susceptible, while the barley cultivars were resistant or moderately resistant. lentil, field pea, fenugreek, linseed, and medic were found to be resis ...200019271015
pratylenchus thornei associated with reduced wheat yield in oregon.pratylenchus thornei reaches high population densities in non-irrigated annual cropping systems in low-rainfall regions of the pacific northwest. two spring wheat varieties with different levels of tolerance and susceptibility to p. thornei were treated or not treated with aldicarb in three experiments. grain yield was inversely correlated (p < 0.05) with pre-plant populations of p. thornei in soil and with p. thornei density in mature roots. as population of p. thornei increased, yield of the m ...200519262842
de novo analysis and functional classification of the transcriptome of the root lesion nematode, pratylenchus thornei, after 454 gs flx sequencing.the migratory endoparasitic root lesion nematode pratylenchus thornei is a major pest of the cereals wheat and barley. in what we believe to be the first global transcriptome analysis for p. thornei, using roche gs flx sequencing, 787,275 reads were assembled into 34,312 contigs using two assembly programs, to yield 6,989 contigs common to both. these contigs were annotated, resulting in functional assignments for 3,048. specific transcripts studied in more detail included carbohydrate active en ...201222309969
influence of urea, hydroxyurea, and thiourea on meloidogyne javanica and infected excised tomato roots in culture.urea (u), hydroxyurea (hu), and thiourea (tu), in various concentrations, were added to chemically defined plant tissue culture medium on which meloidogyne javanica was reared on excised tomato roots. concentrations as low as 3 ppm hu or 12 ppm tu inhibited nematode maturation by 70-90% 4 weeks after inoculation, and the coenocytes in the parasitized tissue were poorly developed. gall weight was also inhibited by 50% in cultures treated with 3 and 6 ppm hu. however, exposing juveniles of m. java ...198419295888
studies on anhydrobiosis of pratylenchus thornei.large populations of pratylenchus thornei, a winter pest of cereals, legumes, and potatoes in the northern negev region of israel, survive 7-8 months of summer drought and return to full activity at the beginning of the rainy season. to demonstrate that it survives the summer in an anhydrobiotic state, all developmental stages of p. thornei were exposed to gradually reduced relative humidity (rh) using glycerin water solutions. at 97.7% rh the nematodes were coiled and able to survive exposure t ...198319295813
nematodes in dryland field crops in the semiarid pacific northwest united states.soils and roots of field crops in low-rainfall regions of the pacific northwest were surveyed for populations of plantparasitic and non-plant-parasitic nematodes. lesion nematodes (pratylenchus species) were recovered from 123 of 130 non-irrigated and 18 of 18 irrigated fields. pratylenchus neglectus was more prevalent than p. thornei, but mixed populations were common. population densities in soil were affected by crop frequency and rotation but not by tillage or soil type (p < 0.05). many fiel ...200419262788
interactions of pratylenchus thornei and fusarium oxysporum f. sp. ciceris on chickpea.abstract fusarium oxysporum f. sp. ciceris and the root-lesion nematode pratylenchus thornei coinfect chickpeas in southern spain. the influence of root infection by p. thornei on the reaction of fusarium wilt-susceptible (cps 1 and pv 61) and wilt-resistant (uc 27) chickpea cultivars to f. oxysporum f. sp. ciceris race 5 was investigated under controlled and field conditions. severity of fusarium wilt was not modified by coinfection of chickpeas by p. thornei and f. oxysporum f. sp. ciceris, in ...199818944890
economic impact of hessian fly (diptera: cecidomyiidae) on spring wheat in oregon and additive yield losses with fusarium crown rot and lesion nematode.damage caused by hessian fly, mayetiola destructor (say), was quantified in spring wheat, triticum aestivum l., trials near pendleton and moro, or, during 2001 and 2002. five field experiments were established to examine genetic resistance to fusarium crown rot, fusarium pseudograminearum (o'donnell & aoki), and economic damage by lesion nematode, pratylenchus neglectus (rensch, 1924) (filipjev schuurmanns & stekhoven, 1941) and pratylenchus thornei (sher & allen, 1941). hessian fly became the d ...200415154461
identification of common root-lesion nematode (pratylenchus thornei sher et allen) loci in bread wheat.plant parasitic nematodes are a major biotic cause of wheat-yield loss in temperate wheat-growing regions. a major strategy to develop resistance to root-lesion nematodes (rlns) in wheat is to assess and then exploit their natural genetic variation. this study examines rln (pratylenchus thornei) resistance in 1 middle eastern landrace (aus4930 7.2) and 1 synthetic hexaploid wheat, croc_1/ae. squarrosa (224)//opata (croc), using f2 and f9 populations generated by crossing aus4930 7.2 and croc wit ...200617213914
detection and quantification of pratylenchus thornei in dna extracted from soil using real-time pcr.the root-lesion nematode pratylenchus thornei is one of the most important pests restricting productivity of wheat in the pacific northwest (pnw). it is laborious and difficult to use microscopy to count and identify the nematodes in soils. a sybr green i-based real-time polymerase chain reaction (pcr) assay was developed to detect and quantify this species from dna extracts of soil. a primer set, designed from the internal transcribed spacer region (its1) of rdna, was highly specific to p. thor ...201221879792
gene silencing in root lesion nematodes (pratylenchus spp.) significantly reduces reproduction in a plant host.root lesion nematodes (rlns, pratylenchus species) are a group of economically important migratory endoparasitic plant pathogens that attack host roots of major crops such as wheat and sugarcane, and can reduce crop yields by 7-15%. pratylenchus thornei and pratylenchus zeae were treated with double stranded rna (dsrna) to study gene silencing, (rna interference, rnai), as a potential strategy for their control. mixed stages of nematodes of both species ingested dsrna when incubated in a basic s ...201323201220
characterization of resistance to pratylenchus thornei (nematoda) in wheat (triticum aestivum): attraction, penetration, motility, and reproduction.lines from a cross between two wheat (triticum aestivum) cultivars with contrasting resistance phenotypes to pratylenchus thornei (nematoda) were investigated to determine the stage at which resistance occurs. host resistance was examined at nematode attraction to and penetration of roots and nematode motility, maturation, and reproduction within roots. there was no significant difference in the rate at which p. thornei was attracted toward or penetrated resistant or susceptible roots. however, ...201424047251
qtl for resistance to root lesion nematode (pratylenchus thornei) from a synthetic hexaploid wheat source.a whole genome average interval mapping approach identified eight qtl associated with p. thornei resistance in a dh population from a cross between the synthetic-derived wheat sokoll and cultivar krichauff. pratylenchus thornei are migratory nematodes that feed and reproduce within the wheat root cortex, causing cell death (lesions) resulting in severe yield reductions globally. genotypic selection using molecular markers closely linked to pratylenchus resistance genes will accelerate the develo ...201424748126
nematicidal activity of fluensulfone against some migratory nematodes under laboratory conditions.the fluoroalkenyl fluensulfone, known to have strong nematicidal activity against meloidogyne spp. (root-knot nematodes), was evaluated in vitro and in soil against the migratory nematodes bursaphelenchus xylophilus, aphelenchoides besseyi, aphelenchoides fragariae, ditylenchus dipsaci, pratylenchus penetrans, pratylenchus thornei and xiphinema index.201424452922
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