evaluation of anti-inflammatory activity of some swedish medicinal plants. inhibition of prostaglandin biosynthesis and paf-induced exocytosis.plants used in swedish traditional medicine to treat inflammatory diseases and/or wounds were selected, based on literature data, for evaluation of inhibitory activity on prostaglandin biosynthesis and platelet activating factor (paf)-induced exocytosis in vitro. fifty-nine water extracts from 52 different plants in 28 families were tested. a number of plants, e.g. calluna vulgaris, corylus avellana, geum urbanum, juniperus communis, polygonum aviculare, potentilla erecta and salix caprea were f ...19958583796
an osmotin-like cryoprotective protein from the bittersweet nightshade solanum dulcamara.cold acclimation in plants is a polygenic phenomenon involving increased expression of several genes. the gene products participate either directly or indirectly towards increasing cold tolerance. evidence of proteins having a direct effect on cold tolerance is emerging but limited. with isolated protoplasts from warm-grown kale (brassica oleracea) as a model system, we tested protein fractions from winter bittersweet nightshade, solanum dulcamara, stems for the presence of proteins that have a ...200011198420
[the accumulation and degradation dynamics of cyanophycin in cyanobacteria grown in symbiotic associations with plant tissues and cells].five different artificial associations of cyanobacterial cells with the cells or tissues of nightshade and rauwolfia were studied. the associations grown on nitrogen-containing media produced heterocysts. cyanobacterial cells in the associations retained their ability to take up bound nitrogen from the medium, to store it in the form of cyanophycin granules, and to use them in the process of symbiotic growth. the synthesis and degradation of cyanophycin granules in cyanobacterial cells were more ...200312901011
dual inhibition of 5-lipoxygenase/cyclooxygenase by a reconstituted homeopathic remedy; possible explanation for clinical efficacy and favourable gastrointestinal order to elucidate potential anti-inflammatory activities of zeel comp. n and its constituents, the inhibition of the synthesis of leukotriene b4 (ltb4) and prostaglandin (pge2) by 5-lipoxygenase (5-lox) and cyclo-oxygenase 1 and 2 (cox 1 and 2) respectively were examined in vitro.200415060721
comparative behavior of ralstonia solanacearum biovar 2 in diverse plant species.ralstonia solanacearum causes bacterial wilt in numerous plant species worldwide. although biovar 2 mostly affects solanaceous crops, identification of new hosts remains a matter of concern since there is still no clear-cut distinction between host and nonhost plants. in this work we provide data based on histological studies on the status of 20 plant species, most of them of potential interest in crop rotation. plants were watered with a beta-glucuronidase-expressing derivative of r. solanacear ...200818943239
ralstonia solanacearum requires pops, an ancient avre-family effector, for virulence and to overcome salicylic acid-mediated defenses during tomato pathogenesis.during bacterial wilt of tomato, the plant pathogen ralstonia solanacearum upregulates expression of pops, which encodes a type iii-secreted effector in the avre family. pops is a core effector present in all sequenced strains in the r. solanacearum species complex. the phylogeny of pops mirrors that of the species complex as a whole, suggesting that this is an ancient, vertically inherited effector needed for association with plants. a pops mutant of r. solanacearum uw551 had reduced virulence ...201324281716
application of variable-number tandem-repeat typing to discriminate ralstonia solanacearum strains associated with english watercourses and disease outbreaks.variable-number tandem-repeat (vntr) analysis was used for high-resolution discrimination among ralstonia solanacearum phylotype iib sequevar 1 (piib-1) isolates and further evaluated for use in source tracing. five tandem-repeat-containing loci (comprising six tandem repeats) discriminated 17 different vntr profiles among 75 isolates from potato, geranium, bittersweet (solanum dulcamara), tomato, and the environment. r. solanacearum isolates from crops at three unrelated outbreak sites where ri ...201323892739
a revision of the dulcamaroid clade of  solanum l. (solanaceae).the dulcamaroid clade of solanum contains 45 species of mostly vining or weakly scandent species, including the common circumboreal weed solanum dulcamara l. the group comprises members of the previously recognised infrageneric groupings sect. andropedas rusby, sect. californisolanum a. child, sect. dulcamara (moench) dumort., sect. holophylla (g.don) walp., sect. jasminosolanum (bitter) seithe, sect.lysiphellos (bitter) seithe, subsect. nitidum a.child and sect. subdulcamara dunal. these infrag ...201323794937
discrimination of solanaceae taxa and quantification of scopolamine and hyoscyamine by atr-ftir spectroscopy.plant species of the solanaceae family (nightshades) contain pharmacologically active anticholinergic tropane alkaloids, e.g., scopolamine and hyoscyamine. tropane alkaloids are of special interest, either as active principles or as starting materials for semisynthetic production of other substances. for genetic evaluation, domestication, cultivation, harvest and post-harvest treatments, quantification of the individual active principles is necessary to monitor industrial processes and the resul ...201425248046
bioindication of heavy metals with aquatic macrophytes: the case of a stream polluted with power plant sewages in poland.the kozi brod (left tributary of the biala przemsza, east of katowice) flows in a highly industrial coal-mining area dominated by the power plant of siersza. concentrations of the microelements nickel (ni), chromium (cr), cobalt (co), zinc (zn), manganese (mn), lead (pb), cadmium (cd), copper (cu), barium (ba), aluminum (al), vanadium (v), and strontium (sr), as well as the macronutrients nitrogen (n), phosphorus (p), potassium (k), calcium (ca), magnesium (mg), iron (fe), and sulfur (s), were m ...200111205536
successful treatment of solanum dulcamara intoxication in a labrador retriever puppy.a 10-week-old intact male labrador retriever dog was presented for acute onset of weakness, ataxia, and generalized muscle tremors. the puppy was suffering respiratory and central nervous system (cns) depression, was mildly pyrexic, and vomited plant material that was identified as creeping nightshade (solanum dulcamara). he responded well to supportive care and was discharged successfully. to the authors' knowledge, this is the first report of solanum dulcamara toxicity occurring in a dog.201526663926
purification and characterization of a thermal hysteresis protein from a plant, the bittersweet nightshade solanum dulcamara.thermal hysteresis proteins (thps), which depress the freezing point of water below the melting point (producing a characteristic thermal hysteresis), are well known for their antifreeze activity in both fish and terrestrial arthropods, but have only recently been identified in plants. this study describes the purification of a thp from winter-collected bittersweet nightshade, solanum dulcamara, using ion exchange and preparative 'free flow' isoelectric focusing. the thp has a molecular mass of ...19948186242
characterization of the fungal gibberellin desaturase as a 2-oxoglutarate-dependent dioxygenase and its utilization for enhancing plant growth.the biosynthesis of gibberellic acid (ga(3)) by the fungus fusarium fujikuroi is catalyzed by seven enzymes encoded in a gene cluster. while four of these enzymes are characterized as cytochrome p450 monooxygenases, the nature of a fifth oxidase, ga(4) desaturase (des), is unknown. des converts ga(4) to ga(7) by the formation of a carbon-1,2 double bond in the penultimate step of the pathway. here, we show by expression of the des complementary dna in escherichia coli that des has the characteri ...201222911627
a novel workflow correlating rna-seq data to phythophthora infestans resistance levels in wild solanum species and potato clones.comparative transcriptomics between species can provide valuable understanding of plant-pathogen interactions. here, we focus on wild solanum species and potato clones with varying degree of resistance against phytophthora infestans, which causes the devastating late blight disease in potato. the transcriptomes of three wild solanum species native to southern sweden, solanum dulcamara, solanum nigrum, and solanum physalifolium were compared to three potato clones, desiree (cv.), sw93-1015 and sa ...201526442032
genomic analysis of the native european solanum species, s. dulcamara.solanum dulcamara (bittersweet, climbing nightshade) is one of the few species of the solanaceae family native to europe. as a common weed it is adapted to a wide range of ecological niches and it has long been recognized as one of the alternative hosts for pathogens and pests responsible for many important diseases in potato, such as phytophthora. at the same time, it may represent an alternative source of resistance genes against these diseases. despite its unique ecology and potential as a ge ...201323713999
biological activity of alkaloids from solanum dulcamara l.alkaloids are well known for their antimicrobial activity. though all natural alkaloids come from plants, not all plants produce alkaloids. plants of the solanaceae family are known for their high alkaloid content. alkaloids are found in all plant parts like roots, stems, leaves, flowers, fruits and seeds. in the present study, those plant parts of solanum dulcamara were selected which have been reported to produce a high content of a specific alkaloid: solanine (from unripe fruits), solasodine ...200919418354
biological screening of some turkish medicinal plant extracts for antimicrobial and toxicity activities.screening of antibacterial activity and toxicity of 22 aqueous plant extracts from 17 turkish plants was conducted. antibacterial activity was performed with six bacteria including escherichia coli, pseudomonas aeruginosa, klebsiella pneumoniae, streptococcus pyogenes, staphylococcus aureus and staphylococcus epidermidis. extracts of tussilago farfara leaves, helichyrsum plicatum flowers, solanum dulcamara aerial parts and urtica dioica leaves gave the best inhibitory activity against s. pyogene ...200818075897
convergent evolution within the genus solanum: the specialised anther cone develops through alternative pathways.many angiosperm species produce cones of anthers which release pollen through pores in response to vibration by pollinating bees ("buzz-pollination"). anther cones of varying degrees of strength are a defining morphological trait for the genus solanum. anthers arranged in a robust ('pepper pot') cone are restricted to a single clade within the genus, and may therefore be assumed to be monophyletic. we show that in some species within this clade, such as tomato (solanum lycopersicum), the anther ...200415094186
cloning and characterization of a thermal hysteresis (antifreeze) protein with dna-binding activity from winter bittersweet nightshade, solanum dulcamara.the gene for a thermal hysteresis (antifreeze) protein (sthp-64) from the bittersweet nightshade, solanum dulcamara, was cloned and characterized. an expression cdna library prepared from november s. dulcamara was screened using a polyclonal antibody generated against a previously purified 67 kda thermal hysteresis protein, and positive clones were identified and sequenced. the full-length thermal hysteresis protein gene was cloned into an escherichia coli expression vector and expressed as a fu ...200211905961
induction of dwarfism in transgenic solanum dulcamara by over-expression of a gibberellin 20-oxidase cdna from pumpkin.the gibberellin (ga) 20-oxidase (cmga20ox1) from immature pumpkin seed produces predominantly inactive tricarboxylic acid gas. we expressed cmga20ox1 under the control of the camv 35s promoter in solanum dulcamara to assess the usefulness of this gene for reducing ga content in transgenic plants. all transgenic plants obtained were semi-dwarfs with smaller, deep-green leaves and highly pigmented stems compared to the wild-type. such transformants flowered earlier than the wild-type plants and pr ...200010929126
detection of ralstonia solanacearum, which causes brown rot of potato, by fluorescent in situ hybridization with 23s rrna-targeted probes.during the past few years, ralstonia (pseudomonas) solanacearum race 3, biovar 2, was repeatedly found in potatoes in western europe. to detect this bacterium in potato tissue samples, we developed a method based on fluorescent in situ hybridization (fish). the nearly complete genes encoding 23s rrna of five r. solanacearum strains and one ralstonia pickettii strain were pcr amplified, sequenced, and analyzed by sequence alignment. this resulted in the construction of an unrooted tree and suppor ...19989797321
transformation of solanum dulcamara protoplasts and regeneration of transgenic plants.we have achieved successful transformation of solanum dulcamara protoplasts by direct dna uptake and regeneration of transgenic plants. the plasmids pdw2 carrying cat gene and pcamvneo carrying nptii gene were used. the electroporation voltage was 1500 v, which gave a field strength of 1500 v/cm with a time decay constant of 59.4 sec. the concentration of plasmid was 20 micrograms/2 x 10(6) protoplasts. under these conditions, a very high transformation efficiency was obtained, with relative tra ...19947993970
poisoning in the horse by woody nightshade (solanum dulcamara). 194720270568
the in planta transcriptome of ralstonia solanacearum: conserved physiological and virulence strategies during bacterial wilt of tomato.plant xylem fluid is considered a nutrient-poor environment, but the bacterial wilt pathogen ralstonia solanacearum is well adapted to it, growing to 10(8) to 10(9) cfu/g tomato stem. to better understand how r. solanacearum succeeds in this habitat, we analyzed the transcriptomes of two phylogenetically distinct r. solanacearum strains that both wilt tomato, strains uw551 (phylotype ii) and gmi1000 (phylotype i). we profiled bacterial gene expression at ~6 × 10(8) cfu/ml in culture or in plant ...201222807564
holiday plants with toxic misconceptions.several plants are used for their decorative effect during winter holidays. this review explores the toxic reputation and proposed management for exposures to several of those, namely poinsettia (euphorbia pulcherrima), english holly (ilex aquifolium), american holly (ilex opaca), bittersweet (solanum dulcamara), jerusalem cherry (solanum pseudocapsicum), american mistletoe (phoradendron serotinum), and european mistletoe (viscum album).201223359840
comparative next-generation mapping of the phytophthora infestans resistance gene rpi-dlc2 in a european accession of solanum dulcamara.phytophthora infestans, the causal agent of late blight, remains the main threat to potato production worldwide. screening of 19 accessions of solanum dulcamara with p. infestans isolate ipo82001 in detached leaf assays revealed strong resistance in an individual belonging to accession a54750069-1. this plant was crossed with a susceptible genotype, and an f(1) population consisting of 63 individuals was obtained. this population segregated for resistance in 1:1 ratio, both in detached leaf assa ...201322907632
identification of a resistance gene rpi-dlc1 to phytophthora infestans in european accessions of solanum dulcamara.initial screening of 14 solanum dulcamara accessions enabled the identification of individuals resistant and susceptible to phytophthora infestans. crosses between contrasting genotypes resulted in three f(2)-bc(1) populations segregating for resistance to late blight in a laboratory assay and under field conditions. genetic profiling of one of these populations using 128 aflp primers generated three markers linked to the resistant phenotype. blast analysis of the sequenced markers resulted in a ...201019936699
[sterol glycosides and fatty acid esters of sterol glycosides in the leaves of solanum dulcamara (author's transl)]. 20164776285
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