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comparative study of "micrococcus sp." strains ccm 168 and ccm 1405 and members of the genus salinicoccus.two culture collection strains, ccm 168 and ccm 1405, previously assigned to the genus micrococcus were shown by molecular chemical characterization to belong to the genus salinicoccus. a more detailed comparison of the physiological and biochemical properties of these strains and comparison with the type strain of salinicoccus roseus indicated high degrees of relatedness among the three strains. dna-dna hybridization studies confirmed the high degrees of relatedness. all of the data demonstrate ...19938494738
salinicoccus alkaliphilus sp. nov., a novel alkaliphile and moderate halophile from baer soda lake in inner mongolia autonomous region, china.a novel alkaliphilic and moderately halophilic gram-positive coccus, designated strain t8t, was isolated from baer soda lake in inner mongolia autonomous region, china. strain t8t grew in the presence of 0-25% (w/v) nacl and at ph 6.5-11.5, with optimum growth at 10% (w/v) nacl and ph 9.0. it grew at 10.0-46.0 degrees c, with an optimum growth temperature of 32.0 degrees c. the organism was strictly aerobic, non-motile, non-sporulating and catalase- and oxidase-positive. the dna g+c content was ...200212054239
diversity of moderately halophilic bacteria producing extracellular hydrolytic enzymes.the aim of this study was to determine the diversity of moderately halophilic bacteria with hydrolase activities.200312534822
jeotgalicoccus halotolerans gen. nov., sp. nov. and jeotgalicoccus psychrophilus sp. nov., isolated from the traditional korean fermented seafood jeotgal.two gram-positive, non-motile, non-spore-forming, halotolerant and moderately halophilic cocci (strains ykj-101t and ykj-115t) were isolated from the traditional korean fermented seafood jeotgal, and were investigated using a polyphasic taxonomic approach. phylogenetic analysis of 16s rdna sequences showed that strains ykj-101t and ykj-115t are most closely related to the cluster comprising two salinicoccus species. the peptidoglycan type of the strains is a3alpha, based on l-lys-gly(3-4)-l-ala( ...200312710632
screening and characterization of the protease cp1 produced by the moderately halophilic bacterium pseudoalteromonas sp. strain cp76.a total of 26 proteolytic moderate halophiles were isolated and characterized. most isolates were members of the genus salinivibrio (16 strains), while others were identified as bacillus (4 strains), salinicoccus (2 strains), or members of the gamma-proteobacteria (4 strains). strain cp76 was selected as the best producer of an extracellular protease, designated cp1, and was used for further studies. sequence analysis of the 16s rrna gene in addition to phenotypic tests led to the placement of t ...200312768453
identification of sulfoquinovosyl diacylglycerol as a major polar lipid in marinococcus halophilus and salinicoccus hispanicus and substitution with phosphatidylglycerol.the sulfonolipid sulfoquinovosyl diacylglycerol normally associated with photosynthetic membranes was identified as a major lipid in marinococcus halophilus, salinicoccus hispanicus ("marinococcus hispanicus"), and marinococcus sp. h8 (planococcus sp. h8). phosphatidylglycerol and 0%-10% cardiolipin accounted for the remaining polar lipids in these moderately halophilic, gram-positive bacteria. negative-ion fast atom bombardment mass spectrometry was used to quantify these three polar lipids fro ...200616604117
biodiversity of moderately halophilic bacteria in hypersaline habitats in egypt.screening bacteria from different saline environments in alexandria. egypt, lead to the isolation of 76 gram-negative and 14 gram-positive moderately halophilic bacteria. the isolates were characterized taxonomically for a total of 155 features. these results were analyzed by numerical techniques using simple matching coefficient (ssm) and the clustering was achieved by the unweighed pair-group method of association (upgma). at 75% similarity level the gram-negative bacteria were clustered in 7 ...200616778349
salinicoccus salsiraiae sp. nov.: a new moderately halophilic gram-positive bacterium isolated from salted skate.two moderately halophilic low g + c gram-positive bacteria were isolated from a sample of salted skate (class chondrychthyes, genus raja). phylogenetic analysis of the 16s rrna gene sequence of strains rh1(t) and rh4 showed that these organisms represented a novel species of the genus salinicoccus. the new isolates formed pink-red colonies and flocculated in liquid media, with optimum growth in media containing 4% nacl and ph of about 8.0. these organisms are aerobic but reduce nitrate to nitrit ...200616819559
isolation and initial characterization of the tellurite reducing moderately halophilic bacterium, salinicoccus sp. strain qw6.among the 49 strains of moderately halophilic bacteria isolated from the salty environments of iran, a gram-positive coccus designated as strain qw6 showed high capacity in the removal of toxic oxyanions of tellurium in a wide range of culture medium factors including ph (5.5-10.5), temperature (25-45 degrees c), various salts including nacl, kcl, and na(2)so(4) (0.5-4 m), selenooxyanions (2-10 mm), and at different concentrations of potassium tellurite (0.5-1 mm) under aerobic condition. phenot ...200816971100
salinicoccus jeotgali sp. nov., isolated from jeotgal, a traditional korean fermented seafood.a novel, moderately halophilic, gram-positive coccus, designated strain s2r53-5(t), was isolated from jeotgal, a traditional korean fermented seafood. the organism was strictly aerobic, non-motile, non-sporulating and catalase- and oxidase-positive. strain s2r53-5(t) grew in the presence of 0.5-15 % (w/v) nacl and at ph 6.5-11.0, with optimum growth at 5 % (w/v) nacl and ph 7.0. the temperature range for growth was 20.0-30.0 degrees c, with an optimum temperature of 30 degrees c. phylogenetic an ...200717329798
stability of microbial communities in goat milk during a lactation year: molecular approaches.the microbial communities in milks from one herd were evaluated during 1-year of lactation, using molecular methods to evaluate their stability and the effect of breeding conditions on their composition. the diversity of microbial communities was measured using two approaches: molecular identification by 16s and 18s rdna sequencing of isolates from counting media (two milks), and direct identification using 16s rdna from clone libraries (six milks). the stability of these communities was evaluat ...200717604934
salinicoccus luteus sp. nov., isolated from a desert soil.a moderately halophilic bacterium, strain yim 70202(t), was isolated from a desert soil sample collected from egypt and was subjected to a taxonomic investigation. in a phylogenetic dendrogram based on 16s rrna gene sequence analysis, strain yim 70202(t) was affiliated to the salinicoccus clade, showing 94.5-96.8 % 16s rrna gene sequence similarity to the recognized species of the genus salinicoccus, in which salinicoccus roseus ccm 3516(t) was the nearest neighbour. the dna-dna relatedness valu ...200717684278
salinicoccus siamensis sp. nov., isolated from fermented shrimp paste in thailand.fifteen strains of moderately halophilic, gram-positive cocci were isolated from a traditional fermented shrimp paste ('ka-pi') produced in thailand. these bacteria were strictly aerobic, non-motile, non-sporulating and catalase- and oxidase-positive. they produced orange pigment and grew in the presence of 1.5-25 % (w/v) nacl. they grew optimally in 10 % (w/v) nacl, at ph 8.5 and at 37 degrees c. the cell-wall peptidoglycan was of l-lys type. menaquinone with six isoprene units (mk-6) was a maj ...200717766863
salinicoccus kunmingensis sp. nov., a moderately halophilic bacterium isolated from a salt mine in yunnan, south-west china.a novel yellow-pigmented, non-motile, non-sporulating, catalase- and oxidase-positive, obligately aerobic, moderately halophilic, facultatively alkaliphilic gram-positive coccus, strain yim y15(t), was isolated from a brine sample from a salt mine in yunnan, south-west china. strain yim y15(t) grew in the presence of 0.5-25 % (w/v) nacl and at ph 6.0-10.0, with optimum growth at 8-10 % (w/v) nacl and ph 8.0. it grew at 4-45 degrees c, with optimum growth at 37.0 degrees c. the major cellular fat ...200717911305
[phylogenetic diversity of culturable bacteria in the ancient salt deposits of the yipinglang salt mine, p. r. china].the microbial diversity of cultivable bacteria, isolated from the ancient salt deposits from the yipinglang salt mine (ypl) in the yunnan province, p. r. china,was investigated by using conventional culture-dependent method and phylogenetic analyses based on 16s rrna gene sequence comparisons. 38 bacteria strains were isolated from the brine, halite and saline soil samples on mba (marine broth agar 2216, difco) and isp 2 (international streotomyces project medium 2) media supplemented with 0.5-3 ...200717944352
salinicoccus salitudinis sp. nov., a new moderately halophilic bacterium isolated from a saline soil sample.a novel pale-yellow-pigmented, moderately halophilic, facultatively alkaliphilic, non-motile, non-spore-forming, catalase- and oxidase-positive, obligately aerobic gram-positive coccus, strain yim-c678t was isolated from a saline soil sample collected from a hypersaline habitat in the qaidam basin, northwest china. the organism grew at 4-37 degrees c and ph 6.0-11.0, with optimum growth at 25 degrees c and ph 8.0. strain yim-c678t grew optimally in the presence of 10-12% (w/v) nacl and growth wa ...200818060347
salinicoccus iranensis sp. nov., a novel moderate halophile.a novel moderately halophilic, gram-positive bacterium, designated strain qw6(t), was isolated from textile industry wastewater in qom, iran. strain qw6(t) was strictly aerobic, non-motile, non-sporulating and oxidase- and catalase-positive. it grew at salinities of 1-25% (w/v) nacl, showing optimal growth at 7.5-10.0% (w/v). growth occurred at 5.0-45.0 degrees c and over the ph range 6.5-10.0; growth was optimal at 35 degrees c and ph 7.5. phylogenetic analysis based on 16s rrna gene sequence c ...200818175706
salinicoccus halodurans sp. nov., a moderate halophile from saline soil in china.a moderately halophilic, gram-positive coccus, designated strain w24(t), was isolated from saline soil in qinghai province, china. the isolate was able to grow at salinities of 0-24 % (w/v) nacl (optimally at 8 %, w/v), at ph 5.5-9.0 (optimally at ph 7.5) and at 8-43 degrees c (optimally at 28 degrees c). the genomic dna g+c content of strain w24(t) was 45.8 mol%. the predominant isoprenoid quinone was mk-6 and the cell wall contained lysine and glycine as diagnostic diamino acids. the polar lip ...200818599690
screening and isolation of halophilic bacteria producing extracellular hydrolyses from howz soltan lake, iran.screening of bacteria from different areas of howz soltan playa, a hypersaline lake in the central desert zone of iran, led to the isolation of 231 moderately halophilic bacteria, which were able to grow optimally in media with 5-15% of salt, and 49 extremely halophilic microorganisms that required 20-25% of salt for optimal growth. these isolates produced a great variety of extracellular hydrolytic enzymes. a total of 195, 177, 100, 95, 92, 68, 65, 33, and 28 strains produced lipases, amylases, ...200919037673
nosocomiicoccus ampullae gen. nov., sp. nov., isolated from the surface of bottles of saline solution used in wound cleansing.strains trf-1(t) and tc-9 were isolated from transfer spikes of two separate bottles containing 0.9 % nacl (physiological saline) solution used repeatedly to wash wounds in hospital wards, months apart. phylogenetic analysis of 16s rrna gene sequences revealed that strains trf-1(t) and tc-9 formed a distinct branch within the family bacillaceae most closely related to the members of the genus jeotgalicoccus. the two strains, with identical 16s rrna gene sequences, showed sequence similarities of ...200819060087
salinicoccus albus sp. nov., a halophilic bacterium from a salt mine.a novel gram-positive-staining, catalase- and oxidase-positive, non-motile, non-spore-forming coccus, designated yim-y21(t), was isolated from a salt mine in yunnan, south-west china. the strain was moderately halophilic, facultatively alkaliphilic and obligately aerobic. colonies were white. growth occurred with 1.0-30.0 % nacl (optimum, 10.0 % nacl), at ph 6.0-10.0 (optimum, ph 8.5) and at 5-40 degrees c (optimum, 25 degrees c). the major amino acid constituents of the cell wall were glycine a ...200919329623
[phylogenetic diversity of cultivable bacteria associated with a sea anemone from coast of the naozhou island in zhanjiang, china].to understand the diversity of cultivable bacteria isolated from a sea anemone collected from the coastal water of the naozbou island in the leizhou bay on south china sea.200919445182
salinicoccus carnicancri sp. nov., a halophilic bacterium isolated from a korean fermented seafood.a novel, moderately halophilic bacterium belonging to the genus salinicoccus was isolated from crabs preserved in soy sauce: a traditional korean fermented seafood. colonies of strain crm(t) were ivory and the cells were non-motile, gram-positive cocci. the organism was non-sporulating, catalase-positive and oxidase-negative. the major fatty acids of strain crm(t) were iso-c(15 : 0) (22.0 %), anteiso-c(15 : 0) (40.6 %) and anteiso-c(17 : 0) (12.1 %). the cell wall peptidoglycan contained lysine ...201019656926
[diversity of culturable bacteria associated with the sea urchin hemicentrotus pulcherrimus from naozhou island].to investigate the diversity of culturable bacteria isolated from the sea urchin hemicentrotus pulcherrimus collected from a tidal flat of naozhou island (20 degrees 52'n - 20 degrees 56'n 110 degrees 33'e - 110 degrees 38'e), leizhou bay, south china sea, china.200920112668
notes on the almost unknown genus jeotgalicoccus.to facilitate isolation and differentiation of the almost entirely unknown jeotgalicoccus spp.201020156307
salinicoccus kekensis sp. nov., a novel alkaliphile and moderate halophile isolated from keke salt lake in qinghai, china.a novel alkaliphilic and moderate halophilic bacterium, designated strain k164(t), was isolated from keke salt lake in qinghai, china. the strain grew with 2.0-20.0% (w/v) nacl, at 4-50 degrees c and ph 6.5-11.5, with an optimum of 8% (w/v) nacl, 37degrees c and ph 10, respectively. the predominant respiratory quinone was menaquinone 6 (mk-6) and the major polar lipid was phosphatidylethanolamine. the major cellular fatty acids were anteiso-c(15:0) and iso-c(15:0). the genomic dna g+c content wa ...201020449768
salinicoccus lutaonensis sp. nov., isolated from a coastal hot spring of green island (lutao), taiwan.a gram-positively behaving coccus (cc-spl15-2(t)) was isolated from a coastal hot spring of green island (lutao), taiwan. by 16s rrna gene sequence similarity studies, it was shown that isolate cc-spl15-2(t) clearly belonged to the genus salinicoccus most closely related to salinicoccus hispanicus (98.3%) and salinicoccus roseus (96.7 %), similarities to all other salinicoccus species was lower than 96.5%. in accordance with characteristics of the genus salinicoccus the quinone system was mainly ...201021037029
salinicoccus qingdaonensis sp. nov., a novel bacterium isolated from coastal seawater of qingdao during a bloom of green algae.a novel gram-positive, white-pigmented, non-motile, non-sporulating, catalase- and oxidase- positive, strictly aerobic coccus, designated strain zxm223t, was isolated from a seawater sample collected from the coast of qingdao. it grew at ph 6.0-10.5 and 0-25.0 % (w/v) nacl, with optimum at ph 8.5 and 3.0 % (w/v) nacl. growth occurred at temperatures of 16-42 ºc (optimum at 28 °c). the major fatty acids were anteiso-c15 : 0 and iso-c15 : 0. mk-6 was the major respiratory quinone. the polar lipids ...201121498663
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