| analysis of synonymous codon usage bias and phylogeny of coat protein gene in banana bract mosaic virus isolates. | the banana is one of the world's most important livelihood crops. banana plants are principally infected by four virus species, banana bunchy top virus (genus babuvirus), cucumber mosaic virus (genus cucumovirus), banana streak virus (genus badnavirus) and banana bract mosaic virus (genus potyvirus). the objective of this study is to understand the codon usage pattern and phylogeny of coat protein gene in different banana bract mosaic virus (bbrmv) isolates. the bbrmv coat protein (cp) gene was ... | 2017 | 28770241 |
| detection of cardamom mosaic virus and banana bract mosaic virus in cardamom using sybr green based reverse transcription-quantitative pcr. | cardamom being perennial, propagated vegetatively, detecting viruses in planting material is important to check the spread of viruses through infected material. thus development of effective and sensitive assay for detection of viruses is need of the time. in this view, assay for the detection of cardamom mosaic virus (cdmv) and banana bract mosaic virus (bbrmv), infecting cardamom was developed using sybr green one step reverse transcription-quantitative pcr (rt-qpcr). the rt-qpcr assay amplifi ... | 2014 | 24426323 |
| genetic diversity and recombination analysis in the coat protein gene of banana bract mosaic virus. | banana bract mosaic virus (bbrmv), a member of the genus potyvirus, family potyviridae, is the causal agent of the bract mosaic disease (bbrmd) that causes serious yield losses in banana and plantain in india and the philippines. in this study, global genetic diversity and molecular evolution of bbrmv based on the capsid protein (cp) gene were investigated. multiple alignments of cp gene of 49 bbrmv isolates showed nucleotide (nt) and amino acid (aa) identity of 79-100 and 80-100 %, respectively ... | 2014 | 24691817 |
| population structure of banana bract mosaic virus reveals recombination and negative selection in the helper component protease (hc-pro) gene. | banana bract mosaic virus (bbrmv) is a serious constraint in the production of banana and plantain in india. in this study, we have cloned, sequenced and analyzed the helper component proteinase (hc-pro) gene of 22 isolates from india and compared with previously reported bbrmv isolates. sequence identity of bbrmv isolates encoding hc-pro gene, were 92-100 % both at the nucleotide (nt) and amino acid level. phylogenetic analysis based on nt sequences of non recombinant isolates showed that tn15, ... | 2014 | 25674623 |
| deep sequencing of banana bract mosaic virus from flowering ginger (alpinia purpurata) and development of an immunocapture rt-lamp detection assay. | banana bract mosaic virus (bbrmv) has never been reported in banana plants in hawaii. in 2010, however, it was detected in a new host, flowering ginger (alpinia purpurata). in this study, we characterize the a. purpurata isolate and study its spread in flowering ginger in hawaii. a laboratory study demonstrated that bbrmv could be transmitted from flowering ginger to its natural host, banana, therefore raising a serious concern about the potential risk to the rapidly growing banana industry of h ... | 2016 | 27038825 |
| biology, etiology, and control of virus diseases of banana and plantain. | banana and plantain (musa spp.), produced in 10.3 million ha in the tropics, are among the world's top 10 food crops. they are vegetatively propagated using suckers or tissue culture plants and grown almost as perennial plantations. these are prone to the accumulation of pests and pathogens, especially viruses which contribute to yield reduction and are also barriers to the international exchange of germplasm. the most economically important viruses of banana and plantain are banana bunchy top v ... | 2015 | 25591881 |
| complete genome sequence of a banana bract mosaic virus isolate infecting the french plantain cv. nendran in india. | the first complete genome sequence of an indian isolate (try) of banana bract mosaic virus (bbrmv) was determined following virus rna extraction from the french plantain cv. nendran (aab). the complete genome was 9711 nucleotides excluding the poly(a) tail and had a genome organization similar to that of a philippine (phi) isolate characterized earlier. when compared to bbrmv-phi, the complete genome sequence of bbrmv-try was 94% identical at the nucleotide level and its ten mature proteins had ... | 2011 | 22134527 |
| purification, properties, and diagnosis of banana bract mosaic potyvirus and its distinction from abaca mosaic potyvirus. | abstract using biochemical, serological, and cytopathological evidence, we have confirmed that banana bract mosaic virus (bbrmv) is a distinct member of the family potyviridae. virions of a philippine isolate of bbrmv were purified from field-infected banana cv. cardaba. particles were approximately 725-nm long, banded at a density equivalent to 1.29 to 1.31 g/ml in cesium chloride equilibrium gradients, and had an a(260/280) of 1.17. yields of about 4 mg/kg were obtained from fresh or frozen le ... | 1997 | 18945091 |
| a reliable ic one-step rt-pcr method for the detection of bbrmv to ensure safe exchange of musa germplasm. | an immunocapture (ic) one-step rt-pcr assay was developed to improve the detection of banana bract mosaic virus (bbrmv) in single and bulked samples of banana plants. in this paper, an atypical strain of bbrmv was described, the bbrmv "ref" strain, and we showed that detection with available bbrmv tools using elisa and rt-pcr approaches was not reliable. primer sets bract n1/nr and n2/nr specific to bbrmv were designed and used in rt-pcr and ic-rt-pcr assays with two commercial kits that allow t ... | 2008 | 18675303 |
| design and application of two novel degenerate primer pairs for the detection and complete genomic characterization of potyviruses. | two pairs of degenerate primers were designed from sequences within the potyviral ci (cifor/cirev) and hc-pro-coding regions (hpfo/hprev), and these were shown to be highly specific to members of the genus potyvirus. using the cifor and cirev primers, three novel potyviruses infecting crop and weed species from vietnam were detected, namely telosma mosaic virus (telmv) infecting telosma (telosma cordata, asclepiadaceae), peace lily mosaic virus (pelmv) infecting peace lily (spathiphyllum patinii ... | 2008 | 17906831 |
| development of a multiplex immunocapture pcr with colourimetric detection for viruses of banana. | a multiplex, immunocapture pcr (m-ic-pcr) was developed for the simultaneous detection of three viruses from crude sap extracts of banana and plantain (musa spp.). a reverse transcription step was required for banana bract mosaic virus and cucumber mosaic virus, which have ssrna genomes. the detection of banana bunchy top virus (ssdna genome) was not adversely affected by inclusion in this step. all the three viruses could be detected simultaneously from a mixed infection. identification and det ... | 2000 | 10996641 |
| characterization and expression of the coat protein-coding region of banana bract mosaic potyvirus, development of diagnostic assays and detection of the virus in banana plants from five countries in southeast asia. | we have sequenced the entire coat protein (cp)-coding region and 5' 162 nucleotides of the 3' untranslated region (utr) of nine different isolates of banana bract mosaic virus (bbrmv) from five different countries. further, we have sequenced the 3' 621 nucleotides of the nib-coding region of a philippines isolate. this is the first report of bbrmv in thailand, vietnam and western samoa. when the sequences of the cp-coding region and 3' utr were compared to each other, variability of between 0.3% ... | 1999 | 10542022 |
| banana bract mosaic virus: characterisation using potyvirus specific degenerate pcr primers. | banana bract mosaic (bbmv) is a relatively new, non-persistently aphid transmitted disease of bananas in the philippines. partially purified preparations from infected plants contained low numbers of flexuous virions, 660 to 760 nm in length, and a 38kda protein, possibly the coat protein, which reacted with a general potyvirus antiserum in western blots. there were insufficient virions for conventional antiserum production or cdna synthesis. therefore, dna was amplified using potyvirus-specific ... | 1995 | 7733824 |