| [the mutagenic action of the dna fragments of bovine adenovirus type 3 (bav-3) containing early e1 (oncogene) and e4 regions]. | a study of mutagenic action of dna fragments of bovine adenovirus type 3 (bav-3), containing early e1 (oncogenic) and e4 regions has been carried out. viral dna was digested with xba 1 restriction endonuclease. the mutagenic effect revealed itself by induction of resistance to 6-mercaptopurine in chinese hamster cells. the data obtained suggest that the early regions e1 and e4 can cause mutagenic effect in mammalian cells. | 1989 | 2760327 |
| the action of the tumour promoter, tpa, on mutagenesis induced by different agents (uv light, chemical and viral mutagens). | the present paper deals with effects of 12-o-tetradecanoylphorbol-13-acetate (tpa) on the frequency of induced mutations to 6-mercaptopurine (6mp) and ouabain resistance in chinese hamster and mouse cells. uv light, bovine adenovirus 3(bav-3) and 5-bromodeoxyuridine (brdu) were used as mutagens. tpa was shown to raise the frequency of gene mutations induced by uv light and bav-3 but it did not enhance the mutagenic effect of brdu. we also examined the ability of bav-3 and brdu to induce tumours ... | 1987 | 3614245 |
| [role of the oncogene in the mutagenic activity of bovine adenovirus type 3]. | the mutagenic and carcinogenic effect of two ecori-fragments of bovine adenovirus type 3 (bav-3) dna inserted into pbr325 has been studied. the c fragment (located between 3,6 and 19,7 map units) contains the viral oncogene, the c fragment (between 44,3 and 63,7 map units) displays no transforming activity. it has been established that oncogene bav-3 statistically true increases the yield of mutants resistant to 6-mercaptopurine (6mp) in chinese hamster cells. the c fragment, pbr325 without vira ... | 1985 | 4040773 |
| [mutagens and the tumor promoter]. | we studied the effect of 12-o-tetradecanoylphorbol-13-acetate (tpa) and saccharin on the frequency of induced mutations of resistance to 6-mercaptopurine and ouabain in chinese hamster and mouse cells. uv-rays, bovine adenovirus-3 (bav-3) and 5-bromdeoxyuridine (brdu) were used as mutagens. in the case of bav-3 and brdu, we investigated, apart from the mutagenic effect, the tumor-inducing activity of these mutagens in mice, brdu proved to have no carcinogenic effect. the data about the influence ... | 1985 | 4054615 |
| [mutagenic action of the dna of the highly oncogenic bovine adenovirus type 3 (bav-3)]. | the induction of mutations to the 6-mercaptopurine resistance has been studied in chinese hamster cells after transfection with highly oncogenic bovine adenovirus type 3 dna (bav-3). a statistically significant increase in the yield of resistant mutants is induced by viral dna after two days expression time. the value of induction varies from 56,12 x 10(-5) to 70,02 x 10(-5). the comparison of the mutagenic activity of dna and the intact viral particles has revealed that the number of dna molecu ... | 1983 | 6683105 |
| [induction of chromosome aberrations in chinese hamster cells by oncogenic bovine adenovirus type 3]. | | 1980 | 7190444 |
| characteristics of in vitro transformed cells essential for their in vivo survival, selection and metastasizing activity. | two questions were investigated: (1) the dynamics of in vivo selection of tumor cells possessing metastasizing activity (ma) at the time preceding the appearance of visible lung metastases, and (2) the possible connection of in vivo selection of metastatic tumor cells with their susceptibility to h2o2 produced by the cytotoxic oxidative burst of activated neutrophils, monocytes and macrophages. spontaneously in vitro transformed syrian hamster embryo cells (strain sthe), never selected in vivo a ... | 1986 | 3005179 |
| [use of organ cultures of animal trachea and lung for studying respiratory viruses]. | organ cultures of the trachea and the lungs of newborn guinea pigs, rabbits, chickens, cow and pig embryos supported multiplication of influenza a2 and adenovirus type 3 viruses. trachea organ cultures were shown to be more sensitive to the viruses under study. organ cultures of the chicken trachea can be used for diagnosis of respitaroy virus infections. | 1976 | 945643 |
| tumor-specific transplantation antigen(s) of bovine adenoviruses. | protection against bovine adenovirus type 3-induced primary or transplantable tumors was studied in hamsters immunized with bovine adenoviruses, human adenovirus type 12 (a-12), simian adenovirus type 7 (sa7), or chicken-embryo-lethal-orphan (celo) virus. newborn hamsters inoculated with 2.3 times 10-5 plaqueforming units of bovine adenovirus type 3 were given injections of bovine serotypes 1, 2, or 3 during the latent period of tumor development. no hamsters immunized with type 3 and only 47% o ... | 1975 | 803562 |
| faecal virome analysis of wild animals from brazil. | the brazilian cerrado fauna shows very wide diversity and can be a potential viral reservoir. therefore, the animal's susceptibility to some virus can serve as early warning signs of potential human virus diseases. moreover, the wild animal virome of this biome is unknown. based on this scenario, high-throughput sequencing contributes a robust tool for the identification of known and unknown virus species in this environment. in the present study, faeces samples from cerrado birds (psittacara le ... | 2019 | 31480274 |
| gastrointestinal microbial population of turkey (meleagris gallopavo) affected by hemorrhagic enteritis virus. | hemorrhagic enteritis (he) is an acute viral disease that affects avian species, particularly turkeys, compromising their commercial production and having a negative effect on animal welfare. turkey adenovirus 3 (tadv-3), is the main causal agent of the disease. in this study, we considered 3 groups of turkeys to achieve 2 purposes: 1) a preliminary investigation on the microbiota content in the 4 parts of healthy turkey's intestine (group a), namely duodenum, jejunum, ileum, and ceca was done; ... | 2017 | 28938792 |
| vaccination to conserved influenza antigens in mice using a novel simian adenovirus vector, panad3, derived from the bonobo pan paniscus. | among approximately 1000 adenoviruses from chimpanzees and bonobos studied recently, the pan adenovirus type 3 (panad3, isolated from a bonobo, pan paniscus) has one of the best profiles for a vaccine vector, combining potent transgene immunogenicity with minimal pre-existing immunity in the human population. in this study, we inserted into a replication defective panad3 a transgene expressing a fusion protein of conserved influenza antigens nucleoprotein (np) and matrix 1 (m1). we then studied ... | 2013 | 23536756 |
| molecular characterization, phylogeny analysis and pathogenicity of a muscovy duck adenovirus strain isolated in china in 2014. | this study aimed to characterize a novel adenovirus (adv) isolated from diseased muscovy ducks in china. after the adv was successfully propagated in duck embryo fibroblasts, the morphological and physicochemical properties of the virions were studied by electron microscopy and different tests. the results of the analyses were in conformity with adv properties. the full genome sequence was determined and analyzed. the new isolate (named ch-gd-12-2014) shared over 91% sequence identity with duck ... | 2016 | 26989945 |
| genetic and molecular epidemiological characterization of a novel adenovirus in antarctic penguins collected between 2008 and 2013. | antarctica is considered a relatively uncontaminated region with regard to the infectious diseases because of its extreme environment, and isolated geography. for the genetic characterization and molecular epidemiology of the newly found penguin adenovirus in antarctica, entire genome sequencing and annual survey of penguin adenovirus were conducted. the entire genome sequences of penguin adenoviruses were completed for two chinstrap penguins (pygoscelis antarctica) and two gentoo penguins (pygo ... | 2016 | 27309961 |
| a novel adenovirus in chinstrap penguins (pygoscelis antarctica) in antarctica. | adenoviruses (family adenoviridae) infect various organ systems and cause diseases in a wide range of host species. in this study, we examined multiple tissues from chinstrap penguins (pygoscelis antarctica), collected in antarctica during 2009 and 2010, for the presence of novel adenoviruses by pcr. analysis of a 855-bp region of the hexon gene of a newly identified adenovirus, designated chinstrap penguin adenovirus 1 (cspadv-1), showed nucleotide (amino acid) sequence identity of 71.8% (65.5% ... | 2014 | 24811321 |
| in vitro inhibition of influenza virus infection by a crude extract from isatis indigotica root resulting in the prevention of viral attachment. | isatis indigotica root (iir) has been widely used as a chinese medicinal herb to treat regular seasonal influenza over the long history of traditional chinese medicinal practice. however, its inhibitory activities against influenza virus infections along with the associated mechanisms have not been investigated comprehensively. in this study, the chemical nature, mode of action and in vitro anti-influenza activities of a crude extract ( ... | 2011 | 22179315 |
| serologic surveillance for selected viral agents in captive and free-ranging populations of arabian oryx (oryx leucoryx) from saudi arabia and the united arab emirates. | a total of 294 sera collected between 1999 and 2001 from eight captive and one free-ranging herds of arabian oryx (oryx leucoryx) distributed in saudi arabia (sa) and the united arab emirates (uae) were assayed for antibodies against 13 selected viral agents. arabian oryx have been exposed to bluetongue virus (btv), epizootic hemorrhagic disease virus (ehdv), rinderpest virus (rpv), bovine respiratory syncytial virus (brsv), bovine adenovirus 3 (bav-3), cervid herpesvirus-1, foot-and-mouth disea ... | 2005 | 15827212 |
| odocoileus hemionus deer adenovirus is related to the members of atadenovirus genus. | the odocoileus hemionus deer adenovirus (odadv-1) causes systemic and local vasculitis and proves extremely lethal for mule deer. to characterize the virus, part of the genome flanking the fiber gene was cloned and sequenced. the sequence revealed two open-reading frames that mapped to pviii hexon-associated protein precursor and fiber protein of several other adenoviruses. the highest amino acid homology for pviii and fiber was found with the members of the proposed atadenovirus genus: ovine ad ... | 2002 | 12038693 |
| long-term viral serology of semi-free-living and captive ungulates. | between 1973 and 1994, blood samples were collected at whipsnade wild animal park (uk) from three ungulate species kept in enclosures, including 28 european bison (bison bonasus), 37 scimitar-horned oryx (oryx dammah), and 49 père david's deer (elaphurus davidianus), and also from 144 semi-free-living chinese water deer (hydropotes inermis). these samples were tested for the presence of antibodies against three bovine viral diarrhea virus (bvdv)-like strains, three alpha-herpesvirus strains, enz ... | 1998 | 9732031 |
| [seroprevalence of viral infections in llamas (lama glama) in the republic of argentina]. | this study reports the seroprevalence of bovine viral infections in llamas (lama glama) in argentina. this is the first study made in the country including 390 llamas and testing antibodies against eight viruses. samples were collected from nine farms distributed in three different provinces: buenos aires, córdoba and jujuy. the samples were tested for antibodies against eight viruses known to infect cattle: bovine herpesvirus type 1 (bhv-1), bovine viral diarrhea virus (bvdv), bovine adenovirus ... | 1997 | 9229724 |
| pathology and immunogenicity in the cotton rat (sigmodon hispidus) model after infection with a bovine adenovirus type 3 recombinant virus expressing the firefly luciferase gene. | the histopathology of adenovirus pneumonia in cotton rats (sigmodon hispidus) due to bovine adenovirus type 3-luciferase recombinant virus (bad3-luc), which has a 0.7 kb deletion from the early region 3 (e3) replaced with the firefly luciferase gene, was compared with that produced by the parental wild-type (wt) bovine adenovirus type 3 (bad3). after intranasal inoculation of cotton rats with 3 x 10(7) p.f.u. of bad3-luc, the infectious virus titres in the lungs at various times post-infection w ... | 1996 | 8558115 |
| pathogenesis and immunogenicity of bovine adenovirus type 3 in cotton rats (sigmodon hispidus). | intranasal inoculation of cotton rats (sigmodon hispidus) with 10(8) pfu of bovine adenovirus type 3 (bad3) resulted in limited virus replication in the lung and trachea. histopathological changes in the lungs were characterized by necrosis and hyperplasia of bronchiolar epithelium, eosinophilic intranuclear inclusions, pneumocyte type ii hyperplasia in the alveoli, and mild peribronchiolar and perivascular lymphocytic infiltration. immunohistochemically, viral antigens were observed more freque ... | 1995 | 7483256 |
| a seroepidemiologic survey of three pronghorn (antilocapra americana) populations in southeastern idaho, 1975-1977. | sera from 104 adult and 42 fawn pronghorn antelope (antilocapra americana) from southeastern idaho were tested against selected livestock pathogens. the numbers positive/numbers tested (% positive) were as follows: bovine virus diarrhea - adults 2/102 (2), fawns 0/41 (0);; infectious bovine rhinotracheitis - adults 27/101 (27), fawns 9/42 (22); parainfluenza 3 - adults 79/104 (76), fawns 22/42 (52); bovine adenovirus 7 - adults 42/103 (41), fawns 20/48 (48); bovine adenovirus 3 - adults 11/32 (3 ... | 1980 | 6246285 |
| replication of human adenoviruses in guinea pig embryo cells: an ultrastructural study. | guinea pig embryo (gpe) cells showed different degrees of susceptibility to human adenovirus types as determined by virus infectivity assay and electron microscopic examination. adenovirus 2 and 5 induced extensive cellular changes and produced high titers of infectious virus in gpe cells as in human cells. mature progeny virus and protein crystals were observed in both cell types. adenovirus 7 induced some cellular changes in gpe cells but only a small number of cells yielded progeny virus as d ... | 1987 | 3827599 |
| rodents versus pig model for assessing the performance of serotype chimeric ad5/3 oncolytic adenoviruses. | oncolytic adenoviruses (ad) are promising tools for cancer therapeutics. most ad-based therapies utilize species c serotypes, with adenovirus type 5 (ad5) most commonly employed. prior clinical trials demonstrated low efficiency of oncolytic ad5 vectors, mainly due to the absence of ad5 primary receptor (coxsackie and adenovirus receptor, car) on cancer cells. engineering serotype chimeric vectors (ad5/3) to utilize adenovirus type 3 (ad3) receptors has greatly improved their oncolytic potential ... | 2019 | 30744019 |
| immune stability predicts tuberculosis infection risk in a wild mammal. | immunity is one of the most variable phenotypic traits in animals; however, some individuals may show less fluctuation in immune traits, resulting in stable patterns of immune variation over time. it is currently unknown whether immune variation has consequences for infectious disease risk. in this study, we identified moderately stable immune traits in wild african buffalo and asked whether the stability of these traits affected bovine tuberculosis (tb) infection risk. we found that adaptive im ... | 2019 | 31575363 |
| seroprevalence and risk factors for selected respiratory and reproductive tract pathogen exposure in european bison (bison bonasus) in poland. | after the complete extinction from the wild of european bison (bison bonasus) at the beginning of the twentieth century, the worldwide species population was restored to approximately 5500 individuals, with the species however remaining endangered. despite numerous studies on the ecology and genetics of european bison, the threats of infectious diseases have been largely unexamined. the aim of this study was to screen the exposure of the world's largest population of european bison to the pathog ... | 2018 | 29426407 |
| propagation of an avirulent turkey hemorrhagic enteritis virus isolate in chickens. | a series of studies were undertaken to optimize the propagation of hemorrhagic enteritis virus (hev) in specific-pathogen-free (spf) chickens. a total of 562 spf chickens were orally inoculated with an australian avirulent hev isolate of turkey origin at 9, 14, 21, or 28 days of age with 5, 6, 7, or 8 log 10 genomic copies (gc), while 102 chickens served as uninfected controls. no clinical signs were observed in infected chickens. there was an inoculum-dose-dependent increase in the relative spl ... | 2018 | 29620462 |
| from bench to almost bedside: the long road to a licensed ebola virus vaccine. | the ebola virus (ebov) disease epidemic during 2014-16 in west africa has accelerated the clinical development of several vaccine candidates that have demonstrated efficacy in the gold standard nonhuman primate (nhp) model, namely cynomolgus macaques. areas covered: this review discusses the pre-clinical research and if available, clinical evaluation of the currently available ebov vaccine candidates, while emphasizing the translatability of pre-clinical data generated in the nhp model to clinic ... | 2017 | 29148858 |
| isolation and characterization of a novel cervid adenovirus from white-tailed deer (odocoileus virginianus) fawns in a captive herd. | a novel adenovirus, ceadv1, was isolated from buffy coat and nasal swab samples collected from two captive white-tailed deer (odocoileus virginianus) fawns. the isolation was an incidental finding in the course of screening animals for use in a research study on an unrelated pathogen. in the screening process, virus isolation was performed on both nasal swabs and buffy coat samples and cytopathic effect was observed. electron microscopy revealed viral particles with the shape and morphology of a ... | 2017 | 28662929 |
| enteritis in an alpaca (vicugna pacos) associated with a potentially novel adenovirus. | adenovirus-associated enteritis was diagnosed by histopathology of small intestine in a 2-year-old alpaca (vicugna pacos). electron microscopy confirmed intracytoplasmic and intranuclear adenoviral particles within enterocytes. nucleic acid was extracted from paraffin-embedded tissue sections, and a pan-adenovirus nested polymerase chain reaction (pcr) assay was employed to target a partial sequence of the polymerase gene. the pcr product (321 bp) was cloned and sequenced. comparison of the nucl ... | 2012 | 22786976 |
| pathogenesis of a chinese strain of bovine adenovirus type 3 infection in albino guinea pigs. | bovine adenovirus type 3 (bav-3) is considered one of the most important respiratory tract agents of cattle and is widespread among cattle around the world. a bav-3 strain was isolated from a bovine nasal swab for the first time in china in 2009 and named hlj0955. subsequently, bav-3 has frequently been isolated from calves with respiratory diseases in china. to date, only limited study on the pathogenesis of bav-3 infection in cotton rats has been conducted, and the pathogenesis of bav-3 infect ... | 2014 | 25119675 |
| a metagenomics and case-control study to identify viruses associated with bovine respiratory disease. | bovine respiratory disease (brd) is a common health problem for both dairy and beef cattle, resulting in significant economic loses. in order to identify viruses associated with brd, we used a metagenomics approach to enrich and sequence viral nucleic acids in the nasal swabs of 50 young dairy cattle with symptoms of brd. following deep sequencing, de novo assembly, and translated protein sequence similarity searches, numerous known and previously uncharacterized viruses were identified. bovine ... | 2015 | 25740998 |
| turkey hemorrhagic enteritis virus can be titrated but not propagated in chicken embryos. | this study aimed to investigate the feasibility of propagating and titrating hemorrhagic enteritis virus (hev) in chicken embryos. a total of 308 embryonated eggs were used. at 10 days of embryonic age, eggs were inoculated via allantoic sac or chorioallantoic membrane routes with non-heat-treated (live) hev or heat-treated (dead) hev or served as negative controls. allantoic fluid retrieved at 0, 1, 3, 5, and 7 days postinoculation (dpi) was tested for hev by quantitative pcr. inoculation with ... | 2019 | 31251523 |
| recombinant fiber-2 protein protects muscovy ducks against duck adenovirus 3 (dadv-3). | as a novel duck adenovirus 3 (dadv-3) infection caused significant economic losses to the poultry industry in china, there is an urgent need to develop a safe and effective vaccine. in the research, fiber-1 and fiber-2 proteins were expressed and purified, respectively. to evaluate the immunogenicity of the two recombinant proteins, we investigated the igy antibodies and virus-neutralizing antibodies in duck sera. the protective efficacy was evaluated by mortality, virus shedding and histopathol ... | 2019 | 30388631 |
| development of a taqman-based real-time pcr for detecting duck adenovirus 3. | recently, a novel duck adenovirus (designated as duck adenovirus 3, dadv-3) was discovered in muscovy ducks, china. here, we developed a taqman-based real-time pcr assay (qpcr) for the detection of dadv-3 infection. after the optimization of the qpcr conditions, the lower limit of detection for dadv-3 infection was 40.9 copies/μl. no cross-reactivity was observed with other duck-derived pathogens. intra- and inter-assay variability was less than 2.30%. dadv-3 was detected in embryos and newly ha ... | 2018 | 30114433 |