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development of a biological control method against postharvest diseases of citrus fruits.candida oleophila strain o was previously selected for its high and reliable antagonistic activity against botrytis cinerea and penicillium expansum, two important wound pathogens on post-harvest apples. the application of these antagonistic strains on wound pathogens of citrus was more recently undertaken. the efficacy of yeast (applied at several concentrations from 10(5) to 10(8) cfu/ml) was assessed against p. digitatum and p. italicum inoculated after one hours (at a concentration of 10(5), ...200516637158
yeasts isolated from a fungus-growing ant nest, including the description of trichosporon chiarellii sp. nov., an anamorphic basidiomycetous yeast.thirty-nine yeast strains were recovered from a field nest of a primitive and putative novel attine ant species in the genus myrmicocrypta (hymenoptera: formicidae: tribe attini). yeasts isolated from the fungus garden and waste deposit included candida dubliniensis, candida oleophila, cryptococcus haglerorum and hanseniaspora uvarum. in addition, one morphological type was isolated overwhelmingly. sequencing data of partial large-subunit (lsu) rdna and the internal transcribed spacer (its) regi ...201019671730
effectiveness of cymbopogon citratus l. essential oil to inhibit the growth of some filamentous fungi and yeasts.lemon grass (cymbopogon citratus l.) oil has been known as having therapeutic and antibacterial properties, and its antifungal activity is currently the subject of renewed interest. this study aimed to verify the effectivenesses of c. citratus essential oil to inhibit the growth/survival of some fungi (alternaria alternata, aspergillus niger, fusarium oxysporum, and penicillium roquefortii) and yeasts (candida albicans, candida oleophila, hansenula anomala, saccharomyces cerevisiae, schizosaccha ...200919298215
growth inhibition of pathogenic bacteria and some yeasts by selected essential oils and survival of l. monocytogenes and c. albicans in apple-carrot juice.food safety is a fundamental concern of both consumers and the food industry. the increasing incidence of foodborne diseases increases the demand of using antimicrobials in foods. spices and plants are rich in essential oils and show inhibition activity against microorganisms, which are composed of many compounds. in this research, effects of garlic, bay, black pepper, origanum, orange, thyme, tea tree, mint, clove, and cumin essential oils on listeria monocytogenes aufe 39237, escherichia coli ...200919278342
using candida oleophila as a biocontriol agens to prevent foodborne escherichia coli o157 ehec infections.escherichia coli o157:h7 (ehec o157) is a serious pathogen causing haemorrhagic colitis. in this study, inactivation kinetics of inoculated ehec o157 and response of ehec o157 in apple wounds by different concentrations of c. oleophila was investigated. the results presented in this study indicated that ehec o157 could survive and grow in wounded 'fuji' apples and extensively proliferate. water as a decontaminant was ineffective in reducing ehec o157 in wounded apples. c. oleophila could be a vi ...201223420769
population dynamics of postharvest decay antagonists growing epiphytically and within wounds on grapefruit.abstract coating formulations of shellac, sucrose ester, and cellulose were tested that support populations of bacterial and yeast antagonists of postharvest penicillium decay of grapefruit. surface populations of pseudomonas syringae, p. fluorescens, and p. putida were stable between 10(3) and 10(4) cfu/cm(2) on shellacked fruit over 4 months at 13 degrees c, but numbers of the yeast candida oleophila rose steadily from 2.35 x 10(3) to nearly 10(5). through the first 15 days of cold storage, su ...200018944423
induction of resistance to penicillium digitatum in grapefruit by the yeast biocontrol agent candida oleophila.abstract the yeast candida oleophila, the base of the commercial product aspire, is recommended for the control of postharvest decay in citrus and pome fruit. its modes of action include nutrient competition, site exclusion, and direct mycoparasitism. in the present study, we showed that application of candida oleophila to surface wounds or to intact 'marsh seedless' grapefruit elicited systemic resistance against penicillium digitatum, the main postharvest pathogen of citrus fruit. the inductio ...200218942952
[massive isolation of anamorphous ascomycete yeasts candida oleophila from plant phyllosphere].many years of research has confirmed a wide distribution of anamorphous ascomycete yeasts in the phyllosphere of diverse plants of the moscow oblast. based on the standard morphological and physiological criteria, on the results of restriction analysis of the 5.8s-its rdna region, and on the sequencing of the d1d2 region of 26s rdna, these yeasts were identified as candida oleophila montrocher. previous isolation of this species has been rare, possibly due to its incorrect identification. this s ...200718297883
evaluation of olive oil mill wastewater toxicity on the mitochondrial bioenergetics after treatment with candida oleophila.in a previous work the ability of candida oleophila to use phenolic compounds as sole carbon and energy source at high concentrations without an additional carbon source was reported. c. oleophila grown in bioreactor batch cultures in a diluted and sterilized olive oil mill wastewater (omw) caused a significant decrease in the total tannins content but no significant alteration was observed in phenolic acid and fatty acid content. both treated and untreated omws were tested to evaluate the capac ...200818262649
kurtzmaniella gen. nov. and description of the heterothallic, haplontic yeast species kurtzmaniella cleridarum sp. nov., the teleomorph of candida cleridarum.the teleomorph of candida cleridarum was discovered through the detection of conjugation between isolates of a large collection from the nitidulid beetles of the genus carpophilus found in the flowers of various cacti in arizona, usa. the previous oversight of the sexual cycle of this yeast is attributed to the inequality (ca. 5 : 1) of the two mating types. extensive conjugation between compatible mating types is observed after overnight incubation on 5 % malt agar, followed after 3-5 days by t ...200818218961
a box-behnken design for predicting the combined effects of relative humidity and temperature on antagonistic yeast population density at the surface of apples.the objective of this work was to develop models predicting the combined effects of relative humidity (rh, 75-98%), temperature (5-25 degrees c), and initial applied yeast concentration (10(4)-10(8) cfu/ml) on the apple-surface population densities of two biocontrol agents fused against postharvest diseases; the antagonistic yeasts pichia anomala strain k and candida oleophila strain o. experiments were carried out according to a box-behnken matrix. multiple regression analyses showed that both ...200818177962
degradation of a textile reactive azo dye by a combined chemical-biological process: fenton's reagent-yeast.this work presents the results of our studies on the decolorization of aqueous azo dye reactive black 5 (rb5) solution combining an advanced oxidation process (fenton's reagent) followed by an aerobic biological process (mediated by the yeast candida oleophila). under our conditions, initial experiments showed that fenton's process alone, as well as aerobic treatment by c. oleophila alone, exhibited the capacity to significantly decolorize azo dye solutions up to 200 mg/l, within about 1 and 24h ...200717261325
efficacy assessment of candida oleophila (strain o) and pichia anomala (strain k) against major postharvest diseases of citrus fruits in morocco.two yeasts, candida oleophila (strain o) and pichia anomala (strain k), were previously selected for their antagonistic activity against postharvest diseases on apples and pears. the objective of the study was to determine the efficacy of both antagonistic yeast's against wound postharvest pathogens of citrus fruits. the efficacy of both strains (applied at 10(5), 10(6) and 10(8) cfu/ml) was assessed against penicillium digitatum and p. italicum inoculated after one hour (at a concentration of 1 ...200415756846
in situ development and application of cdna-aflp to isolate genes of candida oleophila (strain o) potentially involved in antagonistic properties against botrytis cinerea.the yeast candida oleophila (strain o) presents a high level of protective activity against botrytis cinerea (gray mold) on postharvest apples. the cdna-aflp technique allows the comparison of mrna populations extracted from cells grown in different conditions. in order to isolate yeast genes potentially involved in biological control properties, that technique was applied on strain o cells growing on apple wounds. the biological control properties of 8 c. oleophila strains and strain o were ass ...200415756845
development of real-time pcr using minor groove binding probe to monitor the biological control agent candida oleophila (strain o).a real-time pcr assay using a 3'-minor groove binding (mgb) probe was developed for specific detection and monitoring of candida oleophila (strain o), a biocontrol agent against botrytis cinerea and penicillium expansum, on harvested apples. the application of the rapd technique on c. oleophila strains followed by reproducible sequence characterized amplified region (scar) amplifications allowed the identification of a semi-specific fragment of 244 bp, observed in the profiles of strain o and th ...200515567227
survey of yeasts for antagonistic activity against salmonella poona in cantaloupe juice and wounds in rinds coinfected with phytopathogenic molds.application of yeasts as biocontrol agents to prevent mold decay of fruits and vegetables has been described. we examined 10 yeasts for potential antagonistic activity against survival and growth of salmonella poona in cantaloupe juice and decay by cladosporium cladosporioides and geotrichum candidum in wounds on cantaloupe rind. cantaloupe juice was inoculated using five schemes: salmonella poona only (1.10 log cfu/ml), high (3.93 to 5.21 log cfu/ml) or low populations (1.79 to 3.26 log cfu/ml) ...200415508621
characterization of extracellular lytic enzymes produced by the yeast biocontrol agent candida oleophila.the yeast candida oleophila, the base of the commercial product aspire, is recommended for the control of postharvest decay of citrus and pome fruit. competition for nutrients and space is believed to be the major mode of action. involvement of fungal cell wall-degrading enzymes is also suggested to play a role in the mechanism of action of yeast antagonists. the present study showed that the yeast c. oleophila is capable of producing and secreting various cell wall-degrading enzymes, including ...200414716497
the effect of under- and overexpressed coexg1-encoded exoglucanase secreted by candida oleophila on the biocontrol of penicillium digitatum.the yeast, candida oleophila, is acknowledged for its biocontrol activity against postharvest moulds. however, the mechanism of this activity is not fully understood. one of the conjectured modes of action is associated with extracellular lytic enzymes, such as beta-exoglucanase. the relationship of beta-exoglucanase in the biocontrol activity of c. oleophila was investigated by generating c. oleophila coexg1-knockouts and double-coexg1 transformants. the knockout transformants secreted 0-13% of ...200312845603
non-conventional methods for the control of post-harvest pear diseases.pears are highly perishable products, especially during the post-harvest phase, when considerable losses can occur. among the fungal diseases, blue mold caused by penicillium expansum, grey mould caused by botrytis cinerea, mucor rot caused by mucor piriformis are common on pear fruits. other (weak) pathogens like phialophora malorum, alternaria spp., and cladosporium herbarum tend to infect wounds and senescent fruits. a post-harvest fungicide treatment can reduce decay but effectiveness decrea ...200312694440
citric acid production by candida strains under intracellular nitrogen limitation.a suitable strain and important factors influencing citric acid formation in yeasts were identified. candida oleophila atcc 20177 was chosen as the best citric acid producer from several candida strains. yields of 50 g/l citric acid were produced in shake flask and 80 g/l in fed-batch fermentations with 1.5 and 3 g/l nh(4)cl under non-optimized conditions. ammonium nitrogen was identified as the limiting substrate for citrate formation. citric acid excretion begins a few hours after exhaustion o ...200212382045
cloning and analysis of coexg1, a secreted 1,3-beta-glucanase of the yeast biocontrol agent candida oleophila.lytic enzymes may have a role in the biological control of fungi. the yeast biocontrol agent, candida oleophila, is an excellent subject to research this matter. in the present study, coexg1, which encodes for a secreted 1,3-beta-glucanase, is the first gene to be cloned from c. oleophila. it was isolated from a partial genomic library and analysed. its open reading frame and putative promoter were expressed in baker's yeast, saccharomyces cerevisiae. the reading frame, expressed under the induc ...200212237858
lipid composition of some yeast strains from livingston island, antarctica.yeast strains cryptococcus albidus, cryptococcus laurentii, rhodotorula minuta were isolated from a moss sample. candida oleophila and rhodotorula mucilaginosa were isolated from a soil sample taken from livingston island. antarctica. fatty acid, phospholipid, sterol and tocopherol composition was determined in separated lipid fraction after fermentation in a medium containing glucose, peptone and yeast extract. unsaturated fatty acids, mainly oleic (51-65%) and linoleic (9.5-16.8%), predominate ...200111899472
yeast strains from livingston island, antarctica.five yeast strains were isolated from soil and moss samples from the livingston island (antarctica) and identified according to morphological cultural and physiological characteristics. all strains had an optimum growth temperature of 15 degrees c: none grew above 25 degrees c. they assimilated d-glucose, d-galactose, sucrose, cellobiose, trehalose, 2-keto-d-gluconate, d-xylose, d-ribose and melezitose. four of them were nonfermentative, only one, which formed pseudomycelium fermented glucose, g ...200111899471
a transformation system for the biocontrol yeast, candida oleophila, based on hygromycin b resistance.lithium acetate transformation and electroporation were applied to the biocontrol yeast, candida oleophila. the hygromycin b resistance gene, flanked by the phosphoglycerate kinase promoter and terminator of candida tropicalis, served as the genetic selection marker. the transformation efficiency of electroporation was almost 400 times more efficient than that of the lithium acetate method. while incorporation of dna, flanked by a sequence endogenous to c. oleophila, transpired apparently by hom ...200111795849
transformation of candida oleophila and survival of a transformant on orange fruit under field conditions.histidine auxotrophs of wild-type strain i-182 of candida oleophila, produced using ethyl methanesulfonate, were transformed with plasmids containing the his3, his4 and his5 genes of saccharomyces cerevisiae. histidine auxotrophy was complemented by the his5 gene of s. cerevisiae. stability of the transformants under non-selective conditions and dna gel-blot analysis suggested that the transforming dna had integrated into the c. oleophila genome. there were no detectable physiological difference ...199910022949
a method for studying the population dynamics of candida oleophila on oranges in the grove, using a selective isolation medium and pcr technique.populations of the biological control agent candida oleophila on fruit in an orange grove were monitored by plating fruit washes and peel homogenates on a selective medium, followed by pcr analysis of dna from yeast colonies resembling c. oleophila. the c. oleophila transformant adgus-10 formed colonies on a selective medium that suppressed the development of 99% of the filamentous fungi and the indigenous yeasts, aureobasium (a yeast-like fungus) and rhodotorula. only 6-8 indigenous yeasts/cm2 ...19989880929
temperature adaptation in yeasts: the role of fatty acids.studies on the yeasts candida oleophila, candida utilis, lipomyces starkeyi, rhodosporidium toruloides and saccharomyces cerevisiae revealed the existence of three different temperature adaptation responses involving changes in fatty acid composition. these conclusions were drawn by determining the growth rates, total cellular fatty acid content, fatty acid composition, degree of unsaturation, and the mean chain length of fatty acids over a range of growth temperatures. within temperatures permi ...19902262787
sequential application of nahco3, cacl2 and candida oleophila (isolate 13l) affects significantly penicillum expansum growth and the infection degree in apples.the employment of biocontrol agents to restrain postharvest pathogens is an encouraging approach, although, efficacy and consistency are still below those of synthetic pesticides. up to date, the 'integrated control strategy' seems to be the most promising way to overcome this gap. here, we report the feasibility to control postharvest decay caused by penicillium expansum in apples by a 2 min, single or sequential, immersion in water with an antagonistic yeast (candida oleophila, isolate '13l'), ...201122702195
[the yeast community associated with the digestive tract of the german cockroach blattella germanica l].data on the yeasts colonizing the digestive tract ofa german cockroach have been first obtained. cockroach cultures are used in the commercial production of allergy vaccines to treat patients sensitized to cockroach allergens. the enteric microflora of the insects can bring nonshared antigens into the composition of the agents manufactured. an investigation established that out of 10 yeast species isolated from the digestive tract of the cockroaches fed sterile food, 6 species (candida glabrata, ...201121797061
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