| nucleotide sequence and replication properties of the bacillus borstelensis cryptic plasmid pht926. | the nucleotide sequence of pht926, a cryptic plasmid found in bacillus borstelensis hp926, was determined. pht926 replicates by a rolling-circle mechanism and belongs to the pc194 plasmid family. the copy number of pht926 was fourfold higher than that of pub110 and very stably maintained in bacillus choshinensis. | 1995 | 7487045 |
| proposal for two new genera, brevibacillus gen. nov. and aneurinibacillus gen. nov. | 16s rrna gene sequences of the type strains of 11 species belonging to the bacillus brevis and bacillus aneurinolyticus groups were determined. on the basis of the results of gene sequence analyses, these species were separated into two clusters. the b. brevis cluster included 10 species, namely, bacillus brevis, bacillus agri, bacillus centrosporus, bacillus choshinensis, bacillus parabrevis, bacillus reuszeri, bacillus formosus, bacillus borstelensis, bacillus laterosporus, and bacillus thermo ... | 1996 | 8863420 |
| complete nucleotide sequence of a cryptic plasmid, pbaw301, from the ruminal anaerobe ruminococcus flavefaciens r13e2. | the complete nucleotide sequence of a cryptic plasmid designated pbaw301, from the gram-positive ruminal bacterium ruminococcus flavefaciens r13e2, has been determined. this plasmid is 1768 bp in size and has an overall g+c content of 43.5%. computer analysis of the sequence data revealed an open reading frame, orf1 (256 amino acids), which is similar to the rep protein of the bacillus borstelensis plasmid pht926. orf1 is preceded by shine-dalgarno and escherichia coli-10 and -35 like sequences. ... | 1996 | 8900066 |
| characterization of a thermostable d-stereospecific alanine amidase from brevibacillus borstelensis bcs-1. | a gene encoding a new thermostable d-stereospecific alanine amidase from the thermophile brevibacillus borstelensis bcs-1 was cloned and sequenced. the molecular mass of the purified enzyme was estimated to be 199 kda after gel filtration chromatography and about 30 kda on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, indicating that the enzyme could be composed of a hexamer with identical subunits. the purified enzyme exhibited strong amidase activity towards d-amino acid-containin ... | 2003 | 12571020 |
| characteristics of a new enantioselective thermostable dipeptidase from brevibacillus borstelensis bcs-1 and its application to synthesis of a d-amino-acid-containing dipeptide. | a new thermostable dipeptidase gene was cloned from the thermophile brevibacillus borstelensis bcs-1 by genetic complementation of the d-glu auxotroph escherichia coli wm335 on a plate containing d-ala-d-glu. nucleotide sequence analysis revealed that the gene included an open reading frame coding for a 307-amino-acid sequence with an m(r) of 35,000. the deduced amino acid sequence of the dipeptidase exhibited 52% similarity with the dipeptidase from listeria monocytogenes. the enzyme was purifi ... | 2004 | 15006780 |
| isolation, characterization, and identification of bacterial contaminants in semifinal gelatin extracts. | bacterial contamination of gelatin is of great concern. indeed, this animal colloid has many industrial applications, mainly in food and pharmaceutical products. in a previous study (e. de clerck and p. de vos, syst. appl. microbiol. 25:611-618), contamination of a gelatin production process with a variety of gram-positive and gram-negative bacteria was demonstrated. in this study, bacterial contamination of semifinal gelatin extracts from several production plants was examined. since these extr ... | 2004 | 15184171 |
| molecular characterisation of bacterial contamination in semi-final gelatine extracts, using denaturing gradient gel electrophoresis. | contamination of gelatine may affect the safety and/or quality of its applications. characterisation of bacterial isolates from semi-final gelatine batches revealed thermotolerant, aerobic, endosporeforming contaminants. in this paper, bacterial contamination in gelatine batches is analysed without previous isolation, by means of denaturing gradient gel electrophoresis (dgge) of pcr-amplified 16s rdna sequences. v9 and v6-v8 regions of the 16s rdna gene were found more suitable for this purpose ... | 2004 | 15490563 |
| biodegradation of polyethylene by the thermophilic bacterium brevibacillus borstelensis. | to select a polyethylene-degrading micro-organism and to study the factors affecting its biodegrading activity. | 2005 | 15836478 |
| flow-injection determination of l-histidine with an immobilized histidine oxidase from brevibacillus borstelensis kait-b-022 and chemiluminescence detection. | a chemiluminometric flow injection analytical system for the quantitation of l-histidine is described. histidine oxidase (ec 1.4.3.-) from brevibacillus borstelensis kait-b-022 was immobilized on tresylated poly(vinyl alcohol) beads and packed into a stainless-steel column. the hydrogen peroxide produced was detected chemiluminometrically by a flowthrough sensor containing immobilized peroxidase (ec 1.1 1.1.7). the maximum sample throughput was 10 h(-1). the calibration graph was linear from 0.0 ... | 2006 | 16429781 |
| brevibacillus ginsengisoli sp. nov., a denitrifying bacterium isolated from soil of a ginseng field. | a gram-positive, rod-shaped, spore-forming bacterium, gsoil 3088t, was isolated from soil from a ginseng field in pocheon province in south korea and characterized in order to determine its taxonomic position. on the basis of 16s rrna gene sequence similarity, strain gsoil 3088t was shown to belong to the family paenibacillaceae, being related to brevibacillus centrosporus (96.6%), brevibacillus borstelensis (96.3%), brevibacillus parabrevis (96.1%), brevibacillus formosus (96.1%), brevibacillus ... | 2006 | 17082408 |
| speciation of gram-positive bacteria in fresh and ambient-stored sub-tropical marine fish. | this study identified gram-positive bacteria in three sub-tropical marine fish species; pseudocaranx dentex (silver trevally), pagrus auratus (snapper) and mugil cephalus (sea mullet). it further elucidated the role played by fish habitat, fish body part and ambient storage on the composition of the gram-positive bacteria. a total of 266 isolates of gram-positive bacteria were identified by conventional biochemical methods, vitek, pcr using genus- and species-specific primers and/or 16s rrna gen ... | 2010 | 20110133 |
| new blends of ethylene-butyl acrylate copolymers with thermoplastic starch. characterization and bacterial biodegradation. | ethylene-butyl acrylate copolymer (eba) with 13% of butyl acrylate content was used to produce blends with 10, 30 and 60% of thermoplastic starch (tps) plasticized with glycerol. ethylene-acrylic acid copolymer (eaa) was used as compatibilizer at 20% content with respect to eba. the blends were characterized by x-ray diffraction, atr-fourier transform infrared spectroscopy (atr-ftir), scanning electron microscopy (sem), water-contact angle measurements (ca), differential scanning calorimetry (ds ... | 2016 | 27261731 |
| characterization of bacteriocin like inhibitory substance produced by a new strain brevibacillus borstelensis ag1 isolated from 'marcha'. | in the present study, a bacterium isolated from marcha- a herbal cake used as traditional starter culture to ferment local wine in north east india, was evaluated for bacteriocin like inhibitory substance production and was tested against six food borne/spoilage causing pathogens viz. listeria monocytogenes mtcc 839, bacillus subtilis mtcc 121, clostridium perfringens mtcc 450, staphylococcus aureus, lactobacillus plantarum and leuconostoc mesenteroides mtcc 107 by using bit/disc method followed ... | 2014 | 25477937 |
| [screening and enzyme production characteristics of thermophilic cellulase-producing strains]. | a total of 6 thermophilic cellulase-producing strainswere isolated from organic garden waste mixed chicken composting at thermophilic period. these isolates were identified as streptomyces thermoviolaceus, s. thermodiastaticus, s. thermocarboxydus, s. albidoflavus, s. thermovulgaris and brevibacillus borstelensis through 16s rrna gene sequence alignment and phylogenetic tree analysis. the cellulose-degrading microbial community has been investigated in few researches so far both at home and abro ... | 2016 | 27548981 |
| draft genome sequence of brevibacillus borstelensis cifa_chp40, a thermophilic strain having biotechnological importance. | brevibacillus borstelensis cifa_chp40 is a thermophilic, strictly aerobic gram positive motile bacteria isolated from the alkaline hot water spring located in the eastern ghats zone of india. it could grow in a wide range of temperature and degrade low-density polythene at 37°c. the strain cifa_chp40 produces essential enzymes like protease, lipase, esterase and amidase at 50°c. here, we report the draft genome sequence of b. borstelensis cifa_chp40 which will provide further insight into the me ... | 2016 | 26958091 |
| brevibacillus borstelensis and streptomyces albogriseolus have roles to play in degradation of herbicide, sulfosulfuron. | use of herbicides, in particular sulfosulfuron, at more than recommended doses has raised major concerns about the health hazards for animals and humans. in the present study, isolation of sulfosulfuron-degrading brevibacillus borstelensis and streptomyces albogriseolus from the field soils in the northwestern region of india was carried out where the use of sulfosulfuron is predominant, and further assessed for their potential to degrade sulfosulfuron individually and together in a consortium f ... | 2016 | 28330318 |
| profiling of brevibacillus borstelensis transcriptome exposed to high temperature shock. | to understand the molecular mechanisms underlying the ability of the bacteria to survive at high temperature, gene expression profile of brevibacillusborstelensis at 55°c during 5 and 10min heat shock period was carried out by high-throughput sequencing technology. a total of 2555 non-redundant transcripts were annotated. a total of 575 genes at 5min and 400 genes at 10min exhibited significant differential expression in response to temperature upshift from 50 to 55°c. genes up-regulated under h ... | 2016 | 26585522 |
| bioremediation of carbendazim, a benzimidazole fungicide using brevibacillus borstelensis and streptomyces albogriseolus together. | excessive use of pesticides in agriculture has resulted in contamination of water resources, air, soil and disruption of biogeochemical cycles. these compounds adversely affect humans and animal health, helpful soil microbes and crop production as well. biodegradation of pesticides by microbes exists in a number of habitats like soil, sediments, surface, ground water, and sludge, etc. in the present study, efforts were made to develop a microbial consortium comprising of streptomyces albogriseol ... | 2015 | 26420048 |
| efficient bio-deodorization of thioanisole by a novel bacterium brevibacillus borstelensis gigan1 immobilized onto different parking materials in twin biotrickling filter. | biological treatment of odorous gas is an alternative to conventional physicochemical processes. a newly-isolated and identified brevibacillus borstelensis gigan1 was seeded on active carbon (ac) and ceramic particle (cp) in a twin biotrickling filter (btf) to comparatively probe the removal performance of gaseous thioanisole, respectively. at empty bed residence time (ebrt) of 66 s, 100% of thioanisole (⩽ 3mg l(-1)) could be removed on ac; while 100% of thioanisole could only be achieved for ⩽ ... | 2015 | 25682227 |
| survey of the transcriptome of brevibacillus borstelensis exposed to low temperature shock. | molecular mechanisms underlying the ability of brevibacillus borstelensis to survive and adapt to various environmentally relevant stresses are poorly understood. to define organism's molecular response to low temperature, gene expression profile of b. borstelensis at 20 °c was carried out by high-throughput sequencing technology. a total of 4579 transcripts with a maximum transcript length of 9919 bp were annotated. gene expression profiling identified 712 genes that were significantly up- or d ... | 2014 | 25151307 |