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assignment of kluyveromyces cellobiovorus nomen nudum to candida intermedia (ciferri & ashford) langeron et guerra.a yeast culture isolated in japan from soil and invalidly described as kluyveromyces cellobiovorus nom. nud. dbvpg 6286 (cbs 7153) was compared for its physiological and morphological properties and by ndna-ndna reassociation experiments with the type strains of several species of the genus kluyveromyces as well as of various candida species exhibiting similar phenotypic profiles. dbvpg 6286 was found to be conspecific with the type strain of candida intermedia (ciferri & ashford) langeron et gu ...19921575468
alkane oxidation by a particulate preparation from candida.the oxidation of decane by a cell-free particulate preparation from candida intermedia was studied. decane is oxidized to decanoate via decanol and decanaldehyde. oxidation of decane to decanol requires molecular oxygen. decanol is oxidized to decanaldehyde by a nicotinamide adenine dinucleotide-linked dehydrogenase differing greatly in specificity from ordinary yeast alcohol dehydrogenase. decanaldehyde is oxidized to decanoate by a nicotinamide adenine dinucleotide-linked dehydrogenase that ox ...19714326743
[teichoic acid in candida intermedia h-30 cells]. 19734722967
[enzymatic oxidation of hydrocarbons in candida intermedia cells]. 19715556043
intergeneric transfer of deoxyribonucleic acid killer plasmids, pgkl1 and pgkl2, from kluyveromyces lactis into saccharomyces cerevisiae by cell fusion.two novel linear deoxyribonucleic acid plasmids, pgkl1 and pgkl2, were isolated from the yeast kluyveromyces lactis. k. lactis strains harboring the pgk1 plasmids killed a certain group of yeasts, including saccharomyces cerevisiae, saccharomyces italicus, saccharomyces rouxii, k. lactis, kluyveromyces thermotolerans, kluyvermyces vanudenii, torulopsis glabrata, candida utilis, and candida intermedia. in this experiment, the pgkl1 and pgkl2 plasmids were intergenerically transferred from a k. la ...19817016841
[evaluation of a new method for antifungal drugs susceptibility testing to yeasts].we compared the etest with a broth microdilution method (fp panel), performed according to the national committee for modified clinical laboratory standards (nccls) document m27-p guidelines, for determining the mics of 81 clinical isolates of yeasts (7 candida albicans, 8 candida glabrata, 10 candida parapsilosis, 6 pichia anomala, 10 candida tropicalis, 4 candida guilliermondii, 4 candida krusei, 6 trichosporon cutaneum, 5 candida ciferrii, 3 candida famata, 4 candida norvegensis, 2 rhodotorul ...200010783576
characterisation of yeast flora isolated from an artisanal portuguese ewes' cheese.the evolution of the yeast flora was studied for an artisanal semi-hard ewes' cheese made from raw milk. mean log10 yeast counts per gram of cheese body ranged from 2.7 to 6.4, with the higher counts observed after a ripening period of 30 days. the yeast population decreased thereafter and, at the end of curing process, reached values similar to those of the beginning. a total of 344 yeasts strains were randomly isolated from the curd and cheese body during the 60 days long ripening period. este ...200011014522
[thermal resistance of yeasts in orange juice at different concentrations of soluble solids].thermal decimal reduction time (d) of rhodotorula rubra (r. rubra) and candida intermedia (c. intermedia) at 63, 64, 65, 66 and 67 degrees c was studied, at soluble solids concentrations of 12, 36 and 52 degrees brix in orange juice. experiments were conducted according to stumbo method. d values for r. rubra were obtained in a range of 3.1 s for d 67 degrees c at 36 degrees brix as a minimum to 209 s at 64 degrees c and 52 degrees brix as a maximum. for c. intermedia, the minimum d value was 3. ...200111678048
yeast species associated with orange juice: evaluation of different identification methods.five different methods were used to identify yeast isolates from a variety of citrus juice sources. a total of 99 strains, including reference strains, were identified using a partial sequence of the 26s rrna gene, restriction pattern analysis of the internal transcribed spacer region (5.8s-its), classical methodology, the rapid yeast plus system, and api 20c aux. twenty-three different species were identified representing 11 different genera. distribution of the species was considerably differe ...200211916718
[chronic lymphatic leukemia resistant to radiotherapy and chemotherapy associated with pulmonary mycosis (by candida intermedia)]. 195413168060
microbial production of optically pure l-iditol by yeast strains.several yeast strains belonging to genus candida were found to selectively hydrogenate l-sorbose with enantiomeric specificity, yielding optically pure l-iditol in the culture broth. the most active strain, isolated from a commercial lemon, was identified as candida intermedia, which produced 50 g of l-iditol per liter from 150 g of l-sorbose per liter during a 5-day fermentation period (35% yield).198316346404
isolation and characterization of microorganisms associated with the traditional sorghum fermentation for production of sudanese kisra.sorghum flour obtained from sudan was mixed with water in a 1:2 (wt/vol) ratio and fermented at 30 degrees c for 24 h. the bacterial populations increased with fermentation time and reached a plateau at approximately 18 h. at the end of 24 h, sorghum batter ph had dropped from 5.95 to 3.95 and the batter had a lactic acid content of 0.80%. the microbial population during the 24 h of fermentation consisted of bacteria (pediococcus pentosaceus, lactobacillus confusus, lactobacillus brevis, lactoba ...199116348551
inhibition of listeria monocytogenes by food-borne yeasts.many bacteria are known to inhibit food pathogens, such as listeria monocytogenes, by secreting a variety of bactericidal and bacteriostatic substances. in sharp contrast, it is unknown whether yeast has an inhibitory potential for the growth of pathogenic bacteria in food. a total of 404 yeasts were screened for inhibitory activity against five listeria monocytogenes strains. three hundred and four of these yeasts were isolated from smear-ripened cheeses. most of the yeasts were identified by f ...200616391059
application of sscp-pcr fingerprinting to profile the yeast community in raw milk salers cheeses.bacteria and yeasts are important sensory factors of raw-milk cheeses as they contribute to the sensory richness and diversity of these products. the diversity and succession of yeast populations in three traditional registered designation of origin (r.d.o.) salers cheeses have been determined by using phenotypic diagnoses and single-strand conformation polymorphism (sscp) analysis. isolates were identified by phenotypic tests and the sequencing of the d1-d2 domains of the 26s rrna gene. ninety- ...200616464699
iron enriched yeast biomass--a promising mineral feed supplement.yeast biomass enriched with iron could represent a new and safer solution for prevention from anaemia development. such an iron source is less toxic and has better absorbability in organisms. the purpose of our research was the determination of the most suitable iron source in the cultivation medium for the yeast saccharomyces cerevisiae, regarding good growth and iron accumulation in cells. iron(iii) citrate, iron(iii) chloride, iron(iii) nitrate and fe-edta complex were used. the uptake of the ...200716935492
stability of microbial communities in goat milk during a lactation year: molecular approaches.the microbial communities in milks from one herd were evaluated during 1-year of lactation, using molecular methods to evaluate their stability and the effect of breeding conditions on their composition. the diversity of microbial communities was measured using two approaches: molecular identification by 16s and 18s rdna sequencing of isolates from counting media (two milks), and direct identification using 16s rdna from clone libraries (six milks). the stability of these communities was evaluat ...200717604934
[identification and susceptibility against fluconazole and albaconazole of 100 yeasts' strains isolated from vaginal discharge].vulvovaginal candidiasis is a condition that affects a great number of fertile women. it is considered the second cause of genital infection after vaginosis due to gam complex. candida albicans is the most frequent isolated species from vaginal discharge. however, sometimes more than one yeast species could be found in the same clinical sample that are more resistant to antifungal drugs. nowadays, it is necessary to identify properly up to species level the isolated microorganism and to determin ...200718095765
comparison of heterologous xylose transporters in recombinant saccharomyces cerevisiae.baker's yeast (saccharomyces cerevisiae) has been engineered for xylose utilization to enable production of fuel ethanol from lignocellulose raw material. one unresolved challenge is that s. cerevisiae lacks a dedicated transport system for pentose sugars, which means that xylose is transported by non-specific hxt transporters with comparatively low transport rate and affinity for xylose.201020236521
application of denaturing high-performance liquid chromatography (dhplc) for yeasts identification in red smear cheese surfaces.to evaluate and optimize the use of denaturing high-performance liquid chromatography (dhplc) for yeasts identification in red smear cheese surfaces.201020477955
assimilation of liquid hydrocarbon by microorganisms. i. mechanism of hydrocarbon uptake.the uptake mechanism of liquid hydrocarbons of low solubility in water was investigated, using microorganisms with different affinities for liquid hydrocarbon. microorganisms which could utilize hydrocarbon were much more adherent to hydrocarbon than those which could not. the adhesive force between candida intermedia ifo 0761 and hydrocarbon was higher than that of candida tropicalis atcc 20336, though both could utilize hydrocarbon; the total hydrocarbon uptake from the drop and accommodation ...1977322743
control of postharvest botrytis fruit rot of strawberry by volatile organic compounds of candida intermedia.a study was conducted to identify volatile organic compounds or volatiles produced by candida intermedia strain c410 using gas chromatography-mass spectrometry, and to determine efficacy of the volatiles of c. intermedia in suppression of conidial germination and mycelial growth of botrytis cinerea and control of botrytis fruit rot of strawberry. results showed that, among 49 volatiles (esters, alcohols, alkenes, alkanes, alkynes, organic acids, ketones, and aldehydes) identified from c. interme ...201121323467
the first case of bloodstream infection by candida intermedia in japan: the importance of molecular identification.a 65-year-old chinese man with diabetes mellitus was admitted to our hospital complaining of bloody sputum, fever, and dyspnea. despite antibiotic treatment, his condition deteriorated, necessitating mechanical ventilation. diffuse alveolar hemorrhage was suspected, and steroid therapy was initiated. although his condition improved and he was extubated, the fever recurred twice, and on both occasions blood cultures yielded yeasts. the yeasts were misidentified as cryptococcus humicola with a com ...201121302127
catheter-related fungemia caused by candida intermedia.candida intermedia is rarely reported as a human pathogen. we report two cases of catheter-related fungemia caused by c. intermedia which were treated successfully with intravenous fluconazole and catheter removal. the isolates were identified by commercial biochemical methods, oligonucleotide array, and partial sequencing analysis of rrna genes.201019497773
assessment of the microbial diversity at the surface of livarot cheese using culture-dependent and independent approaches.the microbial diversity of the surface of a commercial red-smear cheese, livarot cheese, sold on the retail market was studied using culture-dependent and independent approaches. forty yeasts and 40 bacteria from the cheese surface were collected, dereplicated using single-strand conformation polymorphism (sscp) analysis and identified using rrna gene sequencing for the culture-dependent approach. the culture-independent approach involved cloning and sequencing of the 16s rrna gene and sscp anal ...200919481828
five novel candida species in insect-associated yeast clades isolated from neuroptera and other insects.ascomycete yeasts are found commonly in the guts of basidioma-feeding beetles but little is known about their occurrence in the gut of other insects. in this study we isolated 95 yeasts from the gut of adult insects in five neuropteran families (neuroptera: corydalidae, chrysopidae, ascalaphidae, mantispidae and hemerobiidae) and a roach (blattodea: blattidae). based on dna sequence comparisons and other taxonomic characteristics, they were identified as more than 15 species of saccharomycetes a ...200718333508
adsorption of ochratoxin a from grape juice by yeast cells immobilised in calcium alginate beads.grape juice can be easily contaminated with ochratoxin a (ota), one of the known mycotoxins with the greatest public health significance. among the different approaches to decontaminate juice from this mycotoxin, microbiological methods proved efficient, inexpensive and safe, particularly the use of yeast or yeast products. to ascertain whether immobilisation of the yeast biomass would lead to successful decontamination, alginate beads encapsulating candida intermedia yeast cells were used in ou ...201626485316
two glucose/xylose transporter genes from the yeast candida intermedia: first molecular characterization of a yeast xylose-h+ symporter.candida intermedia pycc 4715 was previously shown to grow well on xylose and to transport this sugar by two different transport systems: high-capacity and low-affinity facilitated diffusion and a high-affinity xylose-proton symporter, both of which accept glucose as a substrate. here we report the isolation of genes encoding both transporters, designated gxf1 (glucose/xylose facilitator 1) and gxs1 (glucose/xylose symporter 1) respectively. although gxf1 was isolated by functional complementatio ...200616402921
the oxidative stress response of the yeast candida intermedia to copper, zinc, and selenium exposure.the yeast candida intermedia, as a model organism, was used to examine the links between the metal ions exposure, reactive oxygen species generation and oxidative stress response. to estimate intracellular peroxide and superoxide levels, the fluorescence indicators dihydrorhodamine 123 and dihydroethidium were used, respectively. antioxidant defence systems were investigated by measuring the activity of catalase, glutathione peroxidase and superoxide dismutase and the content of reduced glutathi ...200515812857
evidence of fluconazole-resistant candida species in tortoises and sea turtles.the aim of this study was to evaluate the antifungal susceptibility of candida spp. recovered from tortoises (chelonoidis spp.) and sea turtles (chelonia mydas, caretta caretta, lepidochelys olivacea, eretmochelys imbricata). for this purpose, material from the oral cavity and cloaca of 77 animals (60 tortoises and 17 sea turtles) was collected. the collected specimens were seeded on 2% sabouraud dextrose agar with chloramphenicol, and the identification was carried out by morphological and bioc ...201526363919
high capacity xylose transport in candida intermedia pycc 4715.xylose-utilising yeasts were screened to identify strains with high xylose transport capacity. among the fastest-growing strains in xylose medium, candida intermedia pycc 4715 showed the highest xylose transport capacity. maximal specific growth rate was the same in glucose and xylose media (mu(max)=0.5 h-1, 30 degrees c). xylose transport showed biphasic kinetics when cells were grown in either xylose- or glucose-limited culture. the high-affinity xylose/proton symport system (km = 0.2 mm, vmax ...200312702245
complete genome sequences of the xylose-fermenting candida intermedia strains cbs 141442 and pycc 4715.sustainable biofuel production from lignocellulosic materials requires efficient and complete use of all abundant sugars in the biomass, including xylose. here, we report on the de novo genome assemblies of two strains of the xylose-fermenting yeast candida intermedia: cbs 141442 and pycc 4715.201728385851
analysis of coenzyme q systems, monosaccharide patterns of purified cell walls, and rapd-pcr patterns in the genus kluyveromyces.analysis of the coenzyme q system and the monosaccharide pattern of purified cell walls were used for species characterization in the genus kluyveromyces. all the type strains of the genus possess coenzyme q-6 and the mannose-glucose ('saccharomyces type') cell wall sugar pattern. with the help of random amplified polymorphic dna-polymerase chain reaction analysis 17 species were separated: k. aestuarii, k. africanus, k. bacillisporus, k. blattae, k. delphensis, k. dobzhanski, k. lactis (anamorp ...19968836443
phylogenetic relationships of species of the genus kluyveromyces van der walt (saccharomycetaceae) deduced from the partial sequences of 18s and 26s ribosomal rnas.in order to clarify the phylogenetic relationships of the species classified in the genus kluyveromyces (saccharomycetaceae), three partial base sequences of 18s and 26s rrnas of eighteen strains were determined. the regions determined of the strains corresponded to positions 1451 through 1618 (168 bases) of 18s rrna and to positions 1611 through 1835 (225 bases) and 493 through 622 (130 bases) of a strain (ifo 2376) of saccharomyces cerevisiae. the analyses of the partial base sequences suggest ...19968782399
[a study of the effect of the nature of the carbon source in nutrient medium on the composition of fatty acids of lipids of candida tropicalis and candida intermedia]. 19685700113
[localization of phosphomannan in candida intermedia h-30 cells]. 19734748903
rewiring yeast sugar transporter preference through modifying a conserved protein motif.utilization of exogenous sugars found in lignocellulosic biomass hydrolysates, such as xylose, must be improved before yeast can serve as an efficient biofuel and biochemical production platform. in particular, the first step in this process, the molecular transport of xylose into the cell, can serve as a significant flux bottleneck and is highly inhibited by other sugars. here we demonstrate that sugar transport preference and kinetics can be rewired through the programming of a sequence motif ...201424344268
snow in the city as a spore bank of potentially pathogenic fungi.this study evaluates the role of snow as a specific ecological niche and a vector in fungal spreading with particular emphasis on potential pathogens in seasonally and daily changing conditions. the experimental material was fungi isolated from the atmospheric air, snow cover, and fragments of ice and soil from underneath the snow cover. the total count of microfungi in the air before snowfall, i.e. in the autumn, reached 1756.1 cfu/m(3) on average. after the first snowfalls, it dropped to 85.2 ...201424176713
exploring the optimum conditions for maximizing the microbial growth of candida intermedia by response surface methodology.exploring optimum and cost-efficient medium composition for microbial growth of candida intermedia y-1981 yeast culture growing on whey was studied by applying a multistep response surface methodology. in the first step, plackett-burman (pb) design was utilized to determine the most significant fermentation medium factors on microbial growth. the medium temperature, sodium chloride and lactose concentrations were determined as the most important factors. subsequently, the optimum combinations of ...201424117150
sugar consumption and ethanol fermentation by transporter-overexpressed xylose-metabolizing saccharomyces cerevisiae harboring a xyloseisomerase pathway.four kinds of transporters, hxt1 and hxy7 from saccharomyces cerevisiae, and gxf1 and gxs1 from candida intermedia, were overexpressed in xylose-metabolizing s. cerevisiae harboring a xyloseisomerase-based pathway. overexpression of transporter enhanced sugar consumption and ethanol production, and gxf1 was efficient for ethanol fermentation from both glucose and xylose.201222591844
[the ultrastructure and redox enzyme activity of candida intermedia cells as related to carbon source and growth factors]. 19744463352
yeasts associated with the white shrimp penaeus schmitti, sediment, and water of sepetiba bay, rio de janeiro, brasil.samples were collected in a polluted site with mean fecal coliform counts of the water being 9.0 x 10(2)/100 ml and less polluted areas including commercial fishing areas with fecal coliforms typically below 2.0 x 10(1)/100 ml. yeast populations from the polluted site were 4.6 x 10(2) to 9.2 x 10(3)/g of shrimp intestine, 1.0 x 10(1) to 2.4 x 10(2)/g of sediment and 4.0 x 10(-1) to 4.6 x 10(1)/ml of water, and in the less polluted site 3.0 x 10(1) to 2.3 x 10(2)/g of intestines and 3.0 x 10(-1) ...19892750315
[in vitro investigations of candida strains for itraconazole].results of studies in vitro of the susceptibility of 102 strains of candida type (88--candida albicans, 7--candida intermedia, 3--candida parapsilosis, 1--candida stellatoidea, 3--torulopsis candida), to itraconazole have been reported. the strains were isolated from lesions of the skin and mucous membranes. the strains tested showed no resistance to this drug. itraconazole concentrations inhibiting completely the growth of the yeast-like fungi ranged from 0.02 to 35.0 micrograms/ml of a culture ...19892554367
assimilation of liquid hydrocarbon by microorganisms. ii. growth kinetics.the growth kinetics of a microorganism with high affinity for liquid hydrocarbon which has a low solubility in water was investigated for candida intermedia ifo 0761 in our previous work. the microorganism contained a hydrocarbon pool in and/or on the cell. the transfer of water-soluble substrates to the cell was not the rate-limiting step in the growth of c. intermedia accompanied by clump formation with liquid hydrocarbon. the operating conditions necessary for the oxygen supply for the growth ...1977870104
three novel species of the anamorphic yeast genus candida in the candida intermedia clade found in japan, thailand and taiwan.four strains of yeasts isolated in japan, thailand and taiwan were found to represent three novel species of the genus candida. the three species are located in a clade including candida tsuchiyae, candida thailandica and candida akabanensis in a tree based on the d1/d2 domain sequences of the large subunit rrna genes but clearly differentiated from these relative species. three novel species are proposed for these strains, i. e., candida berkhoutiae sp. nov., for strains st-49(t) (=bcc 7749(t)= ...201121606608
kinetic modelling reveals current limitations in the production of ethanol from xylose by recombinant saccharomyces cerevisiae.saccharomyces cerevisiae lacks the ability to ferment the pentose sugar xylose that is the second most abundant sugar in nature. therefore two different xylose catabolic pathways have been heterologously expressed in s. cerevisiae. whereas the xylose reductase (xr)-xylitol dehydrogenase (xdh) pathway leads to the production of the by-product xylitol, the xylose isomerase (xi) pathway results in significantly lower xylose consumption. in this study, kinetic models including the reactions ranging ...201121642010
the glucose/xylose facilitator gxf1 from candida intermedia expressed in a xylose-fermenting industrial strain of saccharomyces cerevisiae increases xylose uptake in sscf of wheat straw.ethanolic fermentation of lignocellulose raw materials requires industrial xylose-fermenting strains capable of complete and efficient d-xylose consumption. a central question in xylose fermentation by saccharomyces cerevisiae engineered for xylose fermentation is to improve the xylose uptake. in the current study, the glucose/xylose facilitator gxf1 from candida intermedia, was expressed in three different xylose-fermenting s. cerevisiae strains of industrial origin. the in vivo effect on aerob ...201122113025
expression of the gxf1 transporter from candida intermedia improves fermentation performance in recombinant xylose-utilizing saccharomyces cerevisiae.the glucose/xylose facilitator gxf1 from candida intermedia was expressed in the recombinant xylose-fermenting saccharomyces cerevisiae strain tmb 3057. the new strain, tmb 3411, displayed approximately two times lower k (m) for xylose transport compared to a control strain not expressing gxf1. in aerobic batch cultivation, the specific growth rate was significantly higher at low xylose concentration, 4 g/l, when gxf1 was expressed, whereas it remained unchanged at high xylose concentration, 40 ...200919002682
ethanol productions from d-xylose and cellobiose by kluyveromyces cellobiovorus.yeasts capable of fermenting both d-xylose and cellobiose to ethanol were screened. of 213 species of yeasts surveyed, kluyveromyces cellobiovorus sp. nov., a new species belonging to genus of kluyveromyces, was selected as the sole strain. this strain accumulated 32, 22, and 19 g/l of ethanol from 8% glucose, d-xylose, and cellobiose, respectively. it was also shown that this strain produced ethanol from the enzymatic bagasse hydrolysate containing hexoses and pentoses more efficiently than sac ...198518553700
the expression in saccharomyces cerevisiae of a glucose/xylose symporter from candida intermedia is affected by the presence of a glucose/xylose facilitator.two glucose/xylose transporter genes from candida intermedia were recently cloned and characterized: gxf1, which encodes a glucose/xylose facilitator; and gxs1, which encodes a glucose/xylose proton symporter. here we report the functional expression of these transporters in saccharomyces cerevisiae. while gxf1p seems to be fully functional in s. cerevisiae, the symporter gxs1p exhibits very low glucose/xylose transport activity, which could not be ascribed to insufficient production of the prot ...200818524919
yeast diversity in rice-cassava fermentations produced by the indigenous tapirapé people of brazil.the tapirapé people of the tapi'itãwa tribe of brazil produce several fermented foods and beverages, one of which is called 'cauim'. this beverage usually makes up the main staple food for adults and children. several substrates are used in its production, including cassava, rice, corn, maize and peanuts. a fermentation using rice and cassava was conducted, and samples were collected at 4-h intervals for microbial analysis. the yeast population was low at the beginning of the fermentation and re ...200717697080
uncommon opportunistic yeast bloodstream infections from qatar.eleven uncommon yeast species that are associated with high mortality rates irrespective of antifungal therapy were isolated from 17/187 (201 episodes) pediatric and elderly patients with fungemia from qatar. the samples were taken over a 6-year period (january 2004-december 2010). isolated species included kluyveromyces marxianus, lodderomyces elongisporus, lindnera fabianii, candida dubliniensis, meyerozyma guilliermondii, candida intermedia, pichia kudriavzevii, yarrowia lipolytica, clavispor ...201424934803
uptake of chromium(iii) and chromium(vi) compounds in the yeast cell structure.the study presented in this article investigated the influence of different cr(iii) and cr(vi) compounds in the cultivation medium on the uptake and localization of chromium in the cell structure of the yeast candida intermedia. the morphology of the yeast cell surface was observed by the scanning electron microscopy. results demonstrated that the growth inhibitory concentration of cr(iii) in the cultivation medium induced changes in the yeast cell shape and affected the budding pattern, while i ...200414977359
stress response of yeast candida intermedia to cr(vi).stress response of yeast candida intermedia zim 156 exposed to chromium(vi) was investigated. yeast cells were treated with cr(vi) in concentrations of 50, 100, 300 and 500 microm in the mid-exponential growth phase. monitoring of some bioprocess parameters during growth, specifically po(2), showed that cr(vi) addition, specifically in concentration of 100 and partially 50 micromol/l, increased metabolism intensity, which is connected to induced stress responses. furthermore, oxidation of 2',7'- ...200314708086
multiple forms of xylose reductase in candida intermedia: comparison of their functional properties using quantitative structure-activity relationships, steady-state kinetic analysis, and ph studies.the xylose-fermenting yeast candida intermedia produces two isoforms of xylose reductase: one is nadph-dependent (monospecific xylose reductase; msxr), and another is shown here to prefer nadh approximately 4-fold over nadph (dual specific xylose reductase; dsxr). to compare the functional properties of the isozymes, a steady-state kinetic analysis for the reaction d-xylose + nad(p)h + h(+) <--> xylitol + nad(p)(+) was carried out and specificity constants (k(cat)/k(aldehyde)) were measured for ...200314690376
yeasts from canastra cheese production process: isolation and evaluation of their potential for cheese whey fermentation.canastra cheese is a cheese with geographical indication recognized by the brazilian national institute of industrial protection under number ig201002. it is produced in seven municipalities in the state of minas gerais in a region called serra da canastra. in this work, samples of milk, "pingo" (natural starter), whey and canastra cheese were collected on a farm in medeiros-mg/brazil to evaluate the yeast microbiota and select yeasts for whey fermentation to produce ethanol and volatile aromati ...201728290329
d-xylose metabolism by candida intermedia: isolation and characterisation of two forms of aldose reductase with different coenzyme specificities.to study individual enzyme components responsible for the initial step of d-xylose utilisation by the yeast candida intermedia, a two-step protocol has been developed that enables clear-cut separation and isolation of two structurally similar but functionally different aldose reductases (alrs) in high yield. in the first step, the yeast cell extract is fractionated efficiently by biomimetic chromatography using the dye he-3b (reactive red 120) as pseudoaffinity ligand coupled to sepharose cl-4b. ...200010681056
anaerobic and sequential aerobic production of high-titer ethanol and single cell protein from naoh-pretreated corn stover by a genome shuffling-modified saccharomyces cerevisiae strain.in this study, a saccharomyces cerevisiae recombinant strain 14 was constructed through genome shuffling method by transferring the whole genomic dna of candida intermedia strain 23 into a thermo-tolerant s. cerevisiae strain. the recombinant strain 14 combined the good natures of both parent strains that efficiently produced ethanol from glucose and single cell protein from xylose with 54.6% crude protein and all essential amino acids except cysteine at 35°c. importantly, the recombinant strain ...201627416512
improving xylitol production at elevated temperature with engineered kluyveromyces marxianus through over-expressing transporters.three transporter genes including kluyveromyces marxianus aquaglyceroporin gene (kmfps1), candida intermedia glucose/xylose facilitator gene (cigxf1) or glucose/xylose symporter gene (cigxs1) were over-expressed in k. marxianus yzj017 to improve xylitol production at elevated temperatures. the xylitol production of yzj074 that harbored cigxf1 was improved to 147.62g/l in erlenmeyer flask at 42°c. in fermenter, 99.29 and 149.60g/l xylitol were produced from 99.55 and 151.91g/l xylose with product ...201525465792
construction of fast xylose-fermenting yeast based on industrial ethanol-producing diploid saccharomyces cerevisiae by rational design and adaptive evolution.it remains a challenge for recombinant s. cerevisiae to convert xylose in lignocellulosic biomass hydrolysates to ethanol. although industrial diploid strains are more robust compared to laboratory haploid strains, however, industrial diploid s. cerevisiae strains have been less pursued in previous studies. this work aims to construct fast xylose-fermenting yeast using an industrial ethanol-producing diploid s. cerevisiae strain as a host.201324354503
a molecular transporter engineering approach to improving xylose catabolism in saccharomyces cerevisiae.traditional metabolic pathway engineering rarely considers the influence of molecular transport. here, we describe the directed evolution of two heterologous transporters, candida intermedia gxs1 and scheffersomyces stipitis xut3. growth rate on xylose was improved up to 70% by mutant transporter expression. most mutants were found to exhibit vastly improved v(max) values and display an increase in high cell density sugar consumption rates. mixed glucose and xylose fermentations reveal that muta ...201222445945
biocontrol activity of four non- and low-fermenting yeast strains against aspergillus carbonarius and their ability to remove ochratoxin a from grape juice.aspergillus spp. infection of grape may lead to ochratoxin a (ota) contamination in processed beverages such as wine and grape juice. the aim of the current study was to evaluate the biocontrol potential of two non-fermenting (cyberlindnera jadinii 273 and candida friedrichii 778) and two low-fermenting (candida intermedia 235 and lachancea thermotolerans 751) yeast strains against the pathogenic fungus and ota-producer aspergillus carbonarius, and their ability to remove ota from grape juice. t ...201425113045
a mutated xylose reductase increases bioethanol production more than a glucose/xylose facilitator in simultaneous fermentation and co-fermentation of wheat straw.abstract: genetically engineered saccharomyces cerevisiae strains are able to ferment xylose present in lignocellulosic biomass. however, better xylose fermenting strains are required to reach complete xylose uptake in simultaneous saccharification and co-fermentation (sscf) of lignocellulosic hydrolyzates. in the current study, haploid saccharomyces cerevisiae strains expressing a heterologous xylose pathway including either the native xylose reductase (xr) from p. stipitis, a mutated variant o ...201121906329
identification and characterisation of xylanolytic yeasts isolated from decaying wood and sugarcane bagasse in brazil.in this study, yeasts associated with lignocellulosic materials in brazil, including decaying wood and sugarcane bagasse, were isolated, and their ability to produce xylanolytic enzymes was investigated. a total of 358 yeast isolates were obtained, with 198 strains isolated from decaying wood and 160 strains isolated from decaying sugarcane bagasse samples. seventy-five isolates possessed xylanase activity in solid medium and were identified as belonging to nine species: candida intermedia, c. t ...201424748334
phospholipase and proteinase activities of candida spp. isolates from vulvovaginitis in iran.this study aims to characterize phospholipase and proteinase activities of candida isolates from 82 vulvovaginal candidiasis (vvc) and to study the relationship of these activities with vulvovaginitis.201627544321
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