| activity of glutaraldehyde at low concentrations against capsid proteins of poliovirus type 1 and echovirus type 25. | the activity of glutaraldehyde (gta) against capsid proteins of poliovirus type 1 and echovirus type 25 was studied to understand the mode of action of this reagent against enteroviruses. the viruses were treated with gta concentrations ranging from 0.005 to 0.10%. in the poliovirus particles, high-molecular-weight products were formed by 0.05% gta, whereas in the echovirus particles, they were formed at 0.005% gta. these products consist of complexes composed essentially of vp1 and vp3. there s ... | 1992 | 1336351 |
| impact on routine diagnosis of echovirus infections of intratypic differentiation and antigenic variation in echovirus type 25 studied by using monoclonal antibodies. | we studied the biological and antigenic properties of wild strains of echovirus type 25 isolated in france between 1982 and 1987 and compared them with the jv-4 prototype strains isolated in 1957. the wild strains differed from the prototype strain in their cellular tropism. the prototype strain grew readily in five cell lines (mrc5, ma 104, vero, bgm, and ht 29-18), while for wild strains mrc5 and ht 29-18 cells were the most sensitive and supported growth to high titres (between 4.5 and 7.4 50 ... | 1990 | 2229354 |
| comparative sensitivity of the echovirus type 25 jv-4 prototype strain and two recent isolates to glutaraldehyde at low concentrations. | the sensitivity of two recently isolated antigenic variants of echovirus type 25 (montpellier 76-1262 and thionville 86-222) to glutaraldehyde (gta) at low concentrations was compared with that of the jv-4 prototype strain. the purified viruses were treated under the same conditions with gta at concentrations ranging from 0.002 to 0.10%. the wild strains exhibited significantly lower sensitivity to gta than did the prototype strain; with 0.10% gta, a 2 log10 unit reduction was obtained in 5 min ... | 1994 | 8135510 |
| determination of enterovirus serotype inferred from sequence analysis of pcr products. | enterovirus infections are common in neonates. virus isolation is the only diagnostic method to confirm enterovirus serotype infections, however, is not always successful. | 2003 | 12637085 |
| echovirus 25 infections in scotland, 1961-64. | | 1965 | 14337826 |
| virucidal efficacy of glutaraldehyde against enteroviruses is related to the location of lysine residues in exposed structures of the vp1 capsid protein. | glutaraldehyde (gta) is a potent virucidal disinfectant whose exact mode of action against enteroviruses is not understood. earlier reports showed that gta reacts preferentially with the vp1 capsid protein of echovirus 25 and poliovirus 1 and that gta has affinity for exposed lysine residues on proteins. to investigate further the inactivation of enteroviruses by gta, seven strains were selected on the basis of differences in their overall number and the positions of lysine residues in the amino ... | 2004 | 15006797 |
| [serotype distribution of enteroviruses isolated from paediatric cases prediagnosed as aseptic meningitis between 2001-2004 period]. | enteroviruses have major clinical and public health importance and are one of the leading causes of aseptic meningitis. there are many diseases with similar clinical symptoms and cerebrospinal fluid (csf) findings of aseptic meningitis, thus virus isolation and identification is crucial for definitive diagnosis. virological diagnosis is nonetheless important to distinguish between induced meningitis and other treatable causes of disease with a similar clinical picture. a total of 249 samples obt ... | 2005 | 15900836 |
| enterovirus meningitis in brazil, 1998-2003. | acute viral infections of the central nervous system (cns) such as acute flaccid paralysis, meningitis, and encephalitis, are responsible for a high morbidity, particularly in children. non-polio enteroviruses (npev) are known to be responsible for over 80% of viral meningitis in which the etiologic agent is identified. in the present study, we show the frequency of enterovirus meningitis in brazil from december 1998 to december 2003. enterovirus were isolated from 162 (15.8%), of a total of 1,0 ... | 2006 | 16299728 |
| annual report of the australian national poliovirus reference laboratory, 2008. | the australian national poliovirus reference laboratory (nprl) is accredited by the world health organization (who) for the testing of stool specimens from cases of acute flaccid paralysis (afp), a major clinical presentation of poliovirus infection. the nprl, in collaboration with the australian paediatric surveillance unit, co-ordinates surveillance for cases of afp in children in australia, according to criteria recommended by the who. clinical specimens are referred from afp cases in childre ... | 2009 | 20043599 |
| [genetic characterization of echovirus 25 isolated from henan]. | this research firstly reported the molecular analysis of echo25 (entric cytopathic human orphanviruses type 25). to clarify molecular characteristics of the echo25 virus isolates in henan province and its relationship with the rest of world's isolates,the complete vp1 sequences of the 4 isolates in henan were successfully amplified by rt-pcr and were compared with other echo25 isolates available from genbank. compared with the prototype strain jv-4, the nucleotide sequence identity was 79.2%-80. ... | 2010 | 21344747 |
| human parechovirus-3 infection in children, south korea. | human parechoviruses (hpevs) have recently been recognized as important viral pathogens causing sepsis-like illness and meningitis in children, but the data on these infections in korea is limited. klassevirus is emerging as a novel etiologic agent of acute gastroenteritis, but its role in meningitis remains unclear. | 2013 | 23800693 |
| improved diagnosis on a daily basis of enterovirus meningitis using a one-step real-time rt-pcr assay. | the detection of the enterovirus genome in cerebrospinal fluid (csf) by pcr techniques has proved to be more sensitive than traditional cell culture for the diagnosis of enterovirus meningitis. however, pcr assays are time consuming and labor intensive, particularly if separate hybridization steps are used to confirm the specificity of positive findings. the aim of this study was to develop a one-step real-time rt-pcr assay with lightcycler (lc) technology that was sensitive, rapid, and easy to ... | 2004 | 15484276 |
| characterization of echovirus 25 (ecv 25) in the vp1/2a gene junction region. brief report. | genetic relationships among echovirus type 25 (ecv 25) isolates associated with aseptic meningitis in germany 1997/98 and a 40-year-old ecv 25 prototype strain were investigated using rt-pcr and direct sequencing the vp1/2a gene junction region. sequences were compared to each other and to non-polio enterovirus representatives (phylogenetic analysis). the analysis indicated that the sequences of recent isolates have drifted over time distinctly away from the prototype strain sequence. genetic dr ... | 1999 | 10603180 |
| an outbreak of skin rash by echovirus 25 in an infant home. | in september 1980 an outbreak of a skin eruption occurring in an infant home in milan involved 15 children (aged five to 22 months) out of a total of 25. all of them showed a pink maculopapular rash, three had slight fever and eight mild gastroenteric symptoms (diarrhoea). the exanthem lasted three to four days and was distributed over the trunk and the face and only affected the limbs partially. mild gastroenteric symptoms (abdominal pain or diarrhoea) were reported also by five subjects out of ... | 1983 | 6886446 |
| heterogeneity of capsid proteins of echovirus type 25 wild-type strain and prototype strain, studied by using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting. | sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting were used to compare the capsid proteins of 19 antigenic variants of echovirus type 25 wild-type strains isolated in france between 1976 and 1987 with those of the prototype jv-4 reference strain isolated in 1957. immunoblots were developed by using polyclonal sera from rabbits and mice immunized with the reference strain. immunoblotting patterns revealed reactivity only against viral protein vp1 for sera from both anim ... | 1991 | 1774296 |
| use of non-neutralizing monoclonal antibodies in an elisa for intratypic differentiation of 28 echovirus type 25 clinical isolates. | three non-neutralizing monoclonal antibodies were produced and selected against echovirus type 25 jv-4 prototype strain. they were used in an elisa to investigate the intratypic differentiation of 28 wild isolates. clinical isolates fell into seven different groups according to their reactivity patterns in elisa. two of the non-neutralizing monoclonal antibodies, 9e4 and 6d3, were highly specific, while the third, 6c9, may recognize an epitope common to other types of echoviruses. in contrast, m ... | 1992 | 1372613 |
| serologic comparison of hepatitis a antigen to echovirus 25 isolate cr69(076) recovered from a patient with viral hepatitis. | a serologic comparison was performed by immune electron microscopy between hepatitis a antigen (related to the ms-1 strain of hepatitis a virus) and a viral isolate, cr69(076), from the stool of a patient in costa rica with viral hepatitis. cr69(076) was not antigenically related to hepatitis a antigen but was related to echovirus 25. thus, it is unrelated to the ms-1 strain of hepatitis a virus. | 1975 | 186440 |
| analysis of an echovirus 18 outbreak in thuringia, germany: insights into the molecular epidemiology and evolution of several enterovirus species b members. | in october and november 2010, six children and one woman were presented with symptoms of aseptic meningitis in jena, thuringia, germany. enterovirus rna was detected in the cerebrospinal fluid of all patients by rt-pcr, and preliminary molecular typing revealed echovirus 18 (e-18) as causative agent. virus isolates were obtained from stool samples of three patients and several contact persons. again, most isolates were typed as e-18. in addition, coxsackievirus b5 (cv-b5) and echovirus 25 (e-25) ... | 2016 | 27369854 |
| clinical characteristics and molecular epidemiology of enterovirus infection in infants <3 months in a referral paediatric hospital of barcelona. | enterovirus (ev) infection is common in infants, but the information with regard to the molecular epidemiology and the associations between types and clinical variables is very scarce. this study includes 195 children <3 months old with fever, attended from march 2010 to december 2012 in an emergency department of a tertiary paediatric hospital in whom ev infection was confirmed by real-time pcr in blood and/or cerebrospinal fluid. clinical and epidemiological data was prospectively collected. i ... | 2015 | 26003661 |
| complete genome sequence of a new recombinant echovirus 25 strain isolated from a neonatal patient with hand, foot, and mouth disease complicated by encephalitis in beijing, china. | although human echovirus 25 (e-25), a type of the enterovirus b species, is implicated in aseptic meningitis, information on its gene structure, evolution, and virulence are limited. we report here the complete genome sequence of a novel recombinant e-25 strain (e25/2010/chn/bj) isolated from a neonate with hand, foot, and mouth disease complicated by encephalitis in beijing, china in 2010. the complete viral genome consists of 7429 nucleotides (nts), including a 6585-nt open reading frame. phyl ... | 2015 | 25749996 |
| detection and genotyping of enteroviruses in cerebrospinal fluid in patients in victoria, australia, 2007-2013. | genotyping by vp1 fragment polymerase chain reaction (pcr) and nucleic acid sequencing to detect enterovirus (ev) genotypes was performed directly on 729 ev pcr positive cerebrospinal fluid (csf) samples collected between 2007 and 2012 from victorian hospital inpatients. the overall genotype identification rate from csf-positive material was 43%. the four most common genotypes identified were echovirus 6 (24%), echovirus 30 (17%), echovirus 25 (10%), and coxsackievirus a9 (10%), together compris ... | 2014 | 24474149 |
| a novel enterovirus and parechovirus multiplex one-step real-time pcr-validation and clinical experience. | as the number of new enteroviruses and human parechoviruses seems ever growing, the necessity for updated diagnostics is relevant. we have updated an enterovirus assay and combined it with a previously published assay for human parechovirus resulting in a multiplex one-step rt-pcr assay. the multiplex assay was validated by analysing the sensitivity and specificity of the assay compared to the respective monoplex assays, and a good concordance was found. furthermore, the enterovirus assay was ab ... | 2013 | 23845901 |
| construction and characterization of an infectious cdna clone of echovirus 25. | echovirus 25 (e-25) is a member of the enterovirus family and a common pathogen that induces hand, foot, and mouth disease (hfmd), meningitis, skin rash, and respiratory illnesses. in this study, we constructed and characterized an infectious full-length e-25 cdna clone derived from the xm0297 strain, which was the first subgenotype d6 strain isolated in xiamen, china. the 5'-untranslated regions (5'-utr), p3 (3a-3b, 3d) and p3 (3c) regions of this e-25 (xm0297) strain were highly similar to ev- ... | 2015 | 26004198 |
| serological misdiagnosis of acute liver failure associated with echovirus 25 due to immunological similarities to hepatitis a virus and prozone effect. | we describe a case of acute liver failure caused by echovirus 25 (e25) in a previously healthy 2-year-old boy. initial serological studies were consistent with hepatitis a virus (hav), with prozone phenomenon. the similarity of e25 to hav may obscure accurate diagnosis in some cases of hepatitis. | 2015 | 25355762 |
| [meningitis caused by echovirus 25]. | | 1978 | 733077 |
| virus infection and hand papular dermatoses in young children. | four strains of enterovirus and 1 strain of adenovirus were isolated from stool and pharyngeal swabs by inoculation into primary human embryonic kidney cell culture in 25 cases of dorsum manus infantile papular dermatitis. two strains of coxsackie a-9 virus and 1 strain of echovirus-25 were identified. with the isolated virus as antigen, a matched pair study by sera antibody test was carried out in 43 children with papular dermatitis and 40 normal individuals as controls. in 26 cases of coxsacki ... | 1992 | 1333941 |