| transcriptome and proteome of the highly neurotoxic venom of gloydius intermedius. | the venomics of gloydius intermedius were investigated using expressed sequence tags (ests) analyses, 2d gel-electrophoresis combined with maldi-tof/tof, and lc-ms/ms. a total of 1920 ests from the venom gland cdna library were sequenced; 74% of them belonged to toxin-families. the four most abundant families among the toxin transcripts were: serine protease (sp, 36.2%), bradykinin potentiating peptide (25.3%), l-amino acid oxidase (laao, 13.1%), and phospholipase a2 (pla2, 9.9%). moreover, the ... | 2015 | 26278179 |
| molecular evolution and structure-function relationships of crotoxin-like and asparagine-6-containing phospholipases a2 in pit viper venoms. | some myotoxic or neurotoxic pla2s (phospholipases a2) from pit viper venoms contain characteristic n6 substitutions. our survey of the venoms of more than ten pit viper genera revealed that n6-pla2s exist only in limited asian pit vipers of two genera, protobothrops and gloydius, and exist as either monomers or the basic subunits of heterodimers in some new world pit vipers. for the newly identified n6-pla2s, the neuromuscular blocking activities were assayed with the chick biventer cervicis neu ... | 2004 | 15032748 |
| serine protease isoforms in gloydius intermedius venom: full sequences, molecular phylogeny and evolutionary implications. | nine distinct venom serine proteases (vsps) of gloydius intermedius were studied by transcriptomic, sub-proteomic and phylogenetic analyses. their complete amino acid sequences were deduced after expression sequence tag (est) analyses followed by cdna cloning and sequencing. these vsps appear to be paralogs and contain the catalytic triads and 1-4 potential n-glycosylation sites. their relative expression levels evaluated by qpcr were grossly consistent with their est hit-numbers. the major vsps ... | 2017 | 28554670 |
| the complete mitochondrial genome of gloydius intermedius (squamata: viperidae: crotalinae) from china. | the mitochondrial genome sequence of gloydius intermedius is analyzed and presented for the first time. the genome was 17, 226 bp in length and contained 13 protein-coding genes, 2 ribosomal rna genes, 22 transfer rna genes and 2 control region. the overall base composition was a (32.4%), c (28.8%), t (25.9%), and g (12.9%). the base compositions clearly presented the a-c skew, which was most obvious in the protein-coding genes. the extended termination-associated sequence domain, the central co ... | 2016 | 26006286 |
| structures and functions of crotoxin-like heterodimers and acidic phospholipases a2 from gloydius intermedius venom: insights into the origin of neurotoxic-type rattlesnakes. | the cdnas encoding four major phospholipases a2 (pla2s) were sequenced while the expressed sequence tags of gloydius intermedius venom glands were constructed. these pla2s were designated as gintexin-a precursor, gintexin-b, gin-e6a and gin-e6b, respectively. the deduced amino acid sequences of the former two pla2s are 80% and 90% identical to those of crotoxin-a-precursor and crotoxin-b1, respectively. we also purified gintexin-a, gintexin-b, gin-e6a and gin-e6b like pla2 from the venom. the la ... | 2015 | 25252117 |