isolation and characterization of a myotoxic phospholipase a2 from the venom of the arboreal snake bothriechis (bothrops) schlegelii from costa rica.a new myotoxic phospholipase a2 was isolated from the venom of the arboreal snake bothriechis schlegelii (formerly bothrops schlegelii) from costa rica, by ion-exchange chromatography on cm-sephadex. b. schlegelii myotoxin i is a basic protein (pi > 9.3) with a subunit molecular weight of 15 kda, which migrates as a dimer in sodium dodecyl sulfate-polyacrylamide gel electrophoresis under nonreducing conditions. this myotoxin is recognized by antibodies generated against bothrops asper myotoxin i ...19979056257
purification, sequencing, and phylogenetic analyses of novel lys-49 phospholipases a(2) from the venoms of rattlesnakes and other pit vipers.basic phospholipase a(2) homologs with lys49 substitution at the essential ca(2+)-binding site are present in the venom of pit vipers under many genera. however, they have not been found in rattlesnake venoms before. we have now screened for this protein in the venom of rattlesnakes and other less studied pit vipers. by gel filtration chromatography and rp-hplc, lys49-phospholipase-like proteins were purified from the venoms of two rattlers, crotalus atrox and crotalus m. molossus, and five nonr ...200111594738
contributions of the snake venoms of bothrops asper, crotalus simus and lachesis stenophrys to the paraspecificity of the central american polyspecific antivenom (polival-icp).polival-icp antivenom is produced from plasma of horses immunized toward the venoms of bothrops asper, crotalus simus and lachesis stenophrys. the antibody response induced by these venoms confers polival-icp the capacity to neutralize the venoms of the most important central american viperids, including not only homologous venoms (i.e., venoms used as immunogen), but many heterologous venoms (i.e., venoms not used as immunogen). in this work, the individual contributions of homologous venoms to ...201829407870
biochemical and biological characterization of ecuadorian pitviper venoms (genera bothriechis, bothriopsis, bothrops and lachesis).the lethality and enzymatic activities of venoms from six ecuadorian pitvipers (bothriechis schlegelii, bothriopsis punctata, bothriopsis taeniata, bothrops asper, bothrops atrox and lachesis muta) are similar to those of other south and central american species, but with a few exceptions: bothriopsis taeniata venom had no coagulant activity, and bothriechis schlegelii venom had no haemorrhagic activity.19968817816
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