| de novo assessment and review of pan-american pit viper anticoagulant and procoagulant venom activities via kinetomic analyses. | snakebite with hemotoxic venom continues to be a major source of morbidity and mortality worldwide. our laboratory has characterized the coagulopathy that occurs in vitro in human plasma via specialized thrombelastographic methods to determine if venoms are predominantly anticoagulant or procoagulant in nature. further, the exposure of venoms to carbon monoxide (co) or o-phenylhydroxylamine (pha) modulate putative heme groups attached to key enzymes has also provided mechanistic insight into the ... | 2019 | 30736322 |
| snake venomics and toxicological profiling of the arboreal pitviper bothriechis supraciliaris from costa rica. | the genus bothriechis comprises a lineage of nine species of neotropical pitvipers distributed mainly in highlands across middle america, all adapted to arboreal habitats. bothriechis supraciliaris is a relatively recently described species that inhabits the pacific southwest of costa rica, whose venom had never been studied. a proteomic and toxicological profiling of its venom is here reported. proteins or peptides that belong to eleven families were found, with a predominance of bradykinin-pot ... | 2012 | 22333435 |
| characterization and cdna sequence of bothriechis schlegeliil-amino acid oxidase with antibacterial activity. | snake venoms are complex mixtures of proteins including l-amino acid oxidase (laao). a laao (named bslaao) with a mass of 56kda and a theoretical ip of 5.79, was purified from bothriechis schlegelii venom through size-exclusion, ion exchange and affinity chromatography. the entire protein sequence of 498 amino acids, was determined from cdna using reverse-transcribed mrna isolated from venom gland. the enzyme showed dose-dependent inhibition of bacterial growth. bslaao showed inhibitory effect a ... | 2014 | 24875315 |
| the effect of different snake venoms and anti-venoms on thrombin clotting time in human plasma. | crotaline snake species, or pit vipers, are distributed throughout asia and america. while much is known about the clinical effect of these snake venoms, there is a lack of evidence related to the various anti-venoms available and their effectiveness in reversing the effect of different venoms. | 2010 | 19922984 |
| purification, sequencing, and phylogenetic analyses of novel lys-49 phospholipases a(2) from the venoms of rattlesnakes and other pit vipers. | basic phospholipase a(2) homologs with lys49 substitution at the essential ca(2+)-binding site are present in the venom of pit vipers under many genera. however, they have not been found in rattlesnake venoms before. we have now screened for this protein in the venom of rattlesnakes and other less studied pit vipers. by gel filtration chromatography and rp-hplc, lys49-phospholipase-like proteins were purified from the venoms of two rattlers, crotalus atrox and crotalus m. molossus, and five nonr ... | 2001 | 11594738 |
| profiling the venom gland transcriptomes of costa rican snakes by 454 pyrosequencing. | a long term research goal of venomics, of applied importance for improving current antivenom therapy, but also for drug discovery, is to understand the pharmacological potential of venoms. individually or combined, proteomic and transcriptomic studies have demonstrated their feasibility to explore in depth the molecular diversity of venoms. in the absence of genome sequence, transcriptomes represent also valuable searchable databases for proteomic projects. | 2011 | 21605378 |
| snake venomics and antivenomics of the arboreal neotropical pitvipers bothriechis lateralis and bothriechis schlegelii. | we report the comparative proteomic characterization of the venoms of two related neotropical arboreal pitvipers from costa rica of the genus bothriechis, b. lateralis (side-striped palm pit viper) and b. schlegelii (eyelash pit viper). the crude venoms were fractionated by reverse-phase hplc, followed by analysis of each chromatographic fraction by sds-page, n-terminal sequencing, maldi-tof mass fingerprinting, and collision-induced dissociation tandem mass spectrometry of tryptic peptides. the ... | 2008 | 18444672 |
| inhibitory effect of fucoidan on the activities of crotaline snake venom myotoxic phospholipases a(2). | myotoxic phospholipases a(2) account for most of the muscle necrosis that results from envenenomation by crotaline snakes. in this study, we investigated the protective effect of fucoidan, a natural sulfated polysaccharide obtained from the brown seaweed fucus vesiculosus, against the cytotoxic and myotoxic activities of a group of phospholipase a(2) myotoxins from crotaline snake venoms: bothrops asper myotoxins i, ii, iii, and iv, cerrophidion godmani myotoxins i and ii, atropoides nummifer my ... | 2003 | 14599557 |
| isolation and characterization of a myotoxic phospholipase a2 from the venom of the arboreal snake bothriechis (bothrops) schlegelii from costa rica. | a new myotoxic phospholipase a2 was isolated from the venom of the arboreal snake bothriechis schlegelii (formerly bothrops schlegelii) from costa rica, by ion-exchange chromatography on cm-sephadex. b. schlegelii myotoxin i is a basic protein (pi > 9.3) with a subunit molecular weight of 15 kda, which migrates as a dimer in sodium dodecyl sulfate-polyacrylamide gel electrophoresis under nonreducing conditions. this myotoxin is recognized by antibodies generated against bothrops asper myotoxin i ... | 1997 | 9056257 |
| differential susceptibility of c2c12 myoblasts and myotubes to group ii phospholipase a2 myotoxins from crotalid snake venoms. | group ii phospholipase a(2) (pla(2)) myotoxins isolated from viperidae/crotalidae snake venoms induce a rapid cytolytic effect upon diverse cell types in vitro. previous studies suggested that this effect could be more pronounced on skeletal muscle myotubes than on other cell types, including undifferentiated myoblasts. this study utilized the murine skeletal muscle c2c12 cell line to investigate whether differentiated myotubes are more susceptible than myoblasts, and if this characteristic is s ... | 2016 | 15657942 |
| biochemical and biological characterization of ecuadorian pitviper venoms (genera bothriechis, bothriopsis, bothrops and lachesis). | the lethality and enzymatic activities of venoms from six ecuadorian pitvipers (bothriechis schlegelii, bothriopsis punctata, bothriopsis taeniata, bothrops asper, bothrops atrox and lachesis muta) are similar to those of other south and central american species, but with a few exceptions: bothriopsis taeniata venom had no coagulant activity, and bothriechis schlegelii venom had no haemorrhagic activity. | 1996 | 8817816 |
| [molecular cloning and characterization in silico of phospholipase a(2) transcript isolated from lachesis muta peruvian snake venom]. | isolate and characterize in silico gene phospholipase a(2) (pla(2)) isolated from lachesis muta venom of the peruvian amazon. | 2011 | 21308192 |
| clinical syndromes associated with viperidae family snake envenomation in southwestern colombia. | in southwestern colombia there is a notable variety of snakes that belong to the viperidae family (vipers). the particular clinical manifestation related to species is poorly reported. | 2020 | 32879965 |
| contributions of the snake venoms of bothrops asper, crotalus simus and lachesis stenophrys to the paraspecificity of the central american polyspecific antivenom (polival-icp). | polival-icp antivenom is produced from plasma of horses immunized toward the venoms of bothrops asper, crotalus simus and lachesis stenophrys. the antibody response induced by these venoms confers polival-icp the capacity to neutralize the venoms of the most important central american viperids, including not only homologous venoms (i.e., venoms used as immunogen), but many heterologous venoms (i.e., venoms not used as immunogen). in this work, the individual contributions of homologous venoms to ... | 2018 | 29407870 |
| characterization of a novel snake venom component: kazal-type inhibitor-like protein from the arboreal pitviper bothriechis schlegelii. | snake venoms are composed mainly of a mixture of proteins and peptides. notably, all snake venom toxins have been assigned to a small number of protein families. proteomic studies on snake venoms have recently identified the presence of kazal-type inhibitor-like proteins in the neotropical arboreal snakes bothriechis schlegelii and bothriechis supraciliaris. in the present study, a kazal-type component from b. schlegelii, named kazal-type inhibitor-like protein (ktil), has been completely sequen ... | 2016 | 26973135 |
| biochemical and biological characterization of bothriechis schlegelii snake venoms from colombia and costa rica. | snakebites inflicted by the arboreal viperid snake bothriechis schlegelii in humans are characterized by pain, edema, and ecchymosis at the site of the bite, rarely with blisters, local necrosis, or defibrination. herein, a comparative study of bothriechis schlegelii snake venoms from colombia (bsco) and costa rica (bscr) was carried out in order to compare their main activities and to verify the efficacy of bothrops antivenom produced in brazil to neutralize them. biochemical (sds-page and zymo ... | 2016 | 27439537 |