| [thin layer chromatography and extractive technology of panax japonicum c.a. mey. var. major (burk.) c. y. wu et k. m. feng growing in qingba mountain area]. | chemical components of the root, stem and leaves of panax japonicum var. major were compared with those of radix ginseng and radix notoginseng by tlc. the results showed that the total saponin of the root panax japonicum var. major was closer to that of radix ginseng and the total saponin of its stem and leaves was similar to radix notoginseng. a better extractive technology was obtained after isolating and purifying the whole herb of panax japonicum var. major by ethyl alcohol at different conc ... | 1992 | 1482533 |
| reticuloendothelial system-activating polysaccharides from rhizomes of panax japonicus. i. tochibanan-a and -b. | from rhizomes of panax japonicus (araliaceae), two polysaccharides named tochibanan-a and -b, which show reticuloendothelial-potentiating activity in the carbon clearance test in mice, were isolated. the structure of tochibanan-a (molecular mass: 23,000) was elucidated as a linear beta-1,4-d-galactan. tochibanan-b (molecular mass: 40,000) consists of d-galactose (87.1%), l-arabinose, d-glucose and d-galacturonic acid and has a beta-d-(1----4)-linked galactopyranosyl backbone possessing gala-(1-- ... | 1989 | 2611927 |
| [studies on saponins from the leaves of panax japonicus var. bipinnatifidus(seem.)wu et feng]. | two new saponins, bipinnatifidusoside f1(xii) and f2(xiii), were isolated along with eleven known saponins from the dried leaves of panax japonicus var.bipinnatifidus(seem.)wu et feng collected in the range of qinling mountains in china. by spectrometric analysis(fab-ms,1h and 13cnmr,1h-1h cosy), their structures were elucidated as dammar-25(26)-ene-3 beta, 12 beta, 20(s),24 zeta-tetraol-(20-o-beta-d-glucopyranosyl)-3-o- beta-d-glucopyranosyl(1-2)-beta-d-glucopyranoside (bipinnatifidusodie f1) a ... | 1989 | 2618703 |
| [further study on dammarane saponins of leaves of panax japonicus var. major collected in quinling mountains china]. | in continuation of our studies on the leaves of panax japonicus var. major (burk.) wu et feng, two new dammarane saponins are isolated. by means of fab-ms and 13cnmr, their structures were elucidated as dammar-22(23)-ene-3 beta, 6 alpha, 12 beta, 20(s), 24 section-pentaol- (20-o-beta-d-glucopyranosyl)-6-o-alpha-l-rhamnopyranosyl (1-2)-beta-d- glucopyranoside (majorosids f5) and dammar-23(24)-ene-3 beta, 6 alpha, 12 beta, 20(s), 25-pentaol-(20-o-beta-d- glucopyranosyl)-6-o-alpha-l-rhamnopyranosyl ... | 1989 | 2618710 |
| [dammarane-type saponins in leaves of panax collected in sichuan]. | three saponins have been isolated from the leaves of panax japonicus ssp. elegantior var. major (burkill) wu et feng collected in sichuan, china. they were identified as ginsenoside-rd (0.5%), ginsenoside-rg2 (0.025%) and ginsenoside-rg1 (0.016%), respectively, based on the chemical and spectral analytical data obtained. | 1989 | 2625344 |
| studies of panax japonicus fibrinolysis. | the effects of a 70% methanol extract (pme) obtained from the rhizomes of panax japonicus c. a. meyer on experimental thrombosis and fibrinolysis were investigated in vivo and in vitro. pme showed a promotive effect on the activation of the fibrinolytic system as determined by the euglobulin lysis time (elt) assay but was inactive to the inhibitory effect against endotoxin-induced disseminated intravascular coagulation (dic) in rats. pme and its major components, chikusetsusaponin iii, iv, and v ... | 1989 | 2717685 |
| anti-ulcer action of panax japonicus rhizome. | using hcl/ethanol-induced ulcer in rats as a screening model, an anti-ulcer effect was observed for a methanol extract of panax japonicus rhizome, saponin fractions and chikusetsusaponin iii. results suggest that the gastric mucous membrane-protective effect of the methanol extract is likely to be due to the crude saponin fraction and chikusetsusaponin iii. | 1987 | 3586698 |
| saponin composition of rhizomes of panax japonicus collected in south kyushu, japan, and its significance in oriental traditional medicine. | | 1985 | 4092283 |
| inhibitors of cyclic amp phosphodiesterase in panax ginseng c. a. meyer and panax japonicus c. a. meyer. | | 1984 | 6088096 |
| isolation and characterization of ginsenoside-rg2, 20r-prosapogenin, 20s-prosapogenin and delta 20-prosapogenin. chemical studies on saponins of panax ginseng c. a. meyer, third report. | in the course of a chemical study on pure ginsenosides, a ginsenoside-rg2 was isolated as colorless needles, from the lateral root of panax ginseng c. a. meyer. for the characterization of rg2, a chikusetsusaponin-i (ginsenoide-rg2) was isolated as colorless needles from rhizome of panax japonicus c. a. meyer. the optical rotation value of both saponins were opposite to the published data. on the large scale isolation of ginsenosides, trace amount of ginsenoside-rg2 was isolated as crystals. thr ... | 1980 | 7191267 |
| the effect of chinese hepatoprotective medicines on experimental liver injury in mice. | the purpose of this study was to compare the hepatoprotective effects of seven chinese herbal compounds/mixtures on four known hepatotoxicants in mice. these compounds include fulvotomentosides oleanolic acid, total saponins of panax japonicus (jgs), total saponins of panax notoginseng (ngs), sweroside, oxymatrine, and dimethyl dicarboxylate biphenyl (ddb). all have previously been reported to exhibit hepatoprotective effects. acute liver injury was produced in male cf-1 mice by ccl4, acetaminop ... | 1994 | 7934088 |
| inhibitory effect of some triterpenoid saponins on glucose transport in tumor cells and its application to in vitro cytotoxic and antiviral activities. | the effects of some triterpenoid saponins on glucose transport in ehrlich ascites tumor (eat) cells were examined by measuring 2-deoxy-d-glucose (2-dg) uptake. the correlation of the effects with those on the growth of a human t-cell line (mt-4) and the replication of human immunodeficiency virus in mt-4 cells was also studied. chikusetsusaponin ia isolated from rhizomes of panax japonicus c. a. meyer (araliaceae) inhibited the 2-dg uptake (ic50 = 76.3 microm) in a competitive fashion with respe ... | 1994 | 8073091 |
| molecular authentication of panax species. | using conserved plant sequences as primers, the dna sequences in the ribosomal its1-5.8s-its2 region have been amplified and determined for six panax species, p. ginseng c. a. mey. (oriental ginseng), p. quinquefolius l. (american ginseng), p. notoginseng (burkill) f. h. chen (sanchi), p. japonicus c. a. mey. (japanese ginseng), p. trifolius l. and p. major ting, as well as two common adulterants of ginseng, mirabilis jalapa l. and phytolacca acinosa roxb. an authentication procedure based upon ... | 1999 | 10192964 |
| the plant kingdom as a source of anti-ulcer remedies. | phytogenic agents have traditionally been used by herbalists and indigenous healers for the prevention and treatment of peptic ulcer. this article reviews the anti-acid/anti-peptic, gastro-protective and/or anti-ulcer properties of the most commonly employed herbal medicines and their identified active constituents. botanical compounds with anti-ulcer activity include flavonoids (i.e. quercetin, naringin, silymarin, anthocyanosides, sophoradin derivatives) saponins (i.e. from panax japonicus and ... | 2000 | 11113992 |
| dammarane-type triterpene saponins from panax japonicus. | six new dammarane-type saponins (1-6), together with 11 known saponins (7-17), were isolated from ye-sanchi, the underground part of panax japonicus collected in the south of yunnan province, china. their structures were elucidated by chemical and spectroscopic means. | 2002 | 11908976 |
| dammarane-type saponins from panax japonicus and their neurite outgrowth activity in sk-n-sh cells. | four new dammarane-type saponins (1-4), named yesanchinosides g-j, together with nine ginsenosides (5-13) were isolated from ye-sanchi, the underground part of panax japonicus collected in the south of yunnan province, china. their structures were elucidated on the basis of spectroscopic and chemical methods. ginsenosides rb(1) and rb(3) and notoginsenosides r(4) (6) and fa (7) showed significant neurite outgrowth enhancing activities in human neuroblastoma sk-n-sh cells. | 2002 | 12350149 |
| simultaneous determination of triterpene saponins in ginseng drugs by high-performance liquid chromatography. | a hplc method for the simultaneous determination of 11 triterpene saponins with four-type aglycones (protopanaxadiol, protopanaxatriol, ocotillol and oleanolic acid types) in ginseng drugs was developed and validated. using a gradient of acetonitrile and 10 mm k-phosphate buffer (ph 5.80) as the mobile phase and uv detection at 196 nm, more than 18 ginsenosides with different aglycones were separated satisfactorily within 60 min. the detection limits (signal/noise> or =3) were 0.1 microg for gin ... | 2004 | 15305000 |
| [research of ginsenosides in kou zi qi using hplc-ms-ms]. | to analyze the ginsenosides in kou zi qi (rhizomes of panax japonicus c. a. mey. var. major (burkill) c. y. wu et k. m. feng), and to supply evidences for chemotaxanology of panax species and clinical uses of kou zi qi. | 2004 | 15338885 |
| identification of panax species in the herbal medicine preparations using gradient pcr method. | in order to identify the existence of panax species in herbal medicine preparations, the ginseng specific marker primer was selected and created based on the sequence of korean ginseng dna fragment, 359 bp. the gradient pcr was performed on 40 types of the herbal medicines including the 7 types of araliaceae that are in the same family with the panax ginseng using the created ginseng maker primer. as result, panax notoginseng (chinese), panax japonicus (japanese) and panax quinquefolius (america ... | 2005 | 15802808 |
| anti-obesity effects of chikusetsusaponins isolated from panax japonicus rhizomes. | the rhizomes of panax japonicus are used as a folk medicine for treatment of life-style related diseases such as arteriosclerosis, hyperlipidemia, hypertension and non-insulin-dependent diabetes mellitus as a substitute for ginseng roots in china and japan. obesity is closely associated with life-style-related diseases. this study was performed to clarify whether chikusetsusaponins prevent obesity induced in mice by a high-fat diet for 9 weeks. | 2005 | 15811191 |
| suppression of fas-mediated apoptosis of keratinocyte cells by chikusetsusaponins isolated from the roots of panax japonicus. | fas-mediated apoptotic cell death of a human keratinocyte cell line, hacat cells, and of a murine keratinocyte cell line, pam212 cells, was suppressed by pre-treatment with a methanol extract from the roots of panax japonicus c.a. meyer. activity-guided fractionation led to the isolation of chikusetsusaponins iv, iva, v and polysciasaponin p5 as the active ingredients. of these compounds, chikusetsusaponin iv, was most active when applied at a concentration of 12.5 microg/ml. the intracellular h ... | 2006 | 16534721 |
| production of oleanane saponins by callus tissue1 of panax japonicus. | | 1989 | 17262486 |
| antihepatotoxic actions of ginsenosides from panax ginseng roots1. | the antihepatotoxic effects of ginsenosides, saponins from panax ginseng, and their aglycones were investigated utilizing carbon tetrachloride (ccl (4))- and galactosamine (galn)-induced cytotoxicity in primary cultured rat hepatocytes. prominent protective actions were found with 20( s)-ginsenoside-rh (2), 20( r)-ginsenoside-rg (3) and prosapogenin of ginsenoside-ro, 20( r)- and 20( s)-ginsenoside-rs in ccl (4)-produced cytotoxicity, and 20( s)-ginsenoside-rh (1) and prosapogenin of 20( s)-gins ... | 1985 | 17340405 |
| [effects of total saponins of panax japonicus on human leukemic hl-60 cells]. | to study the effects of total saponins of panax japonicus (tspj) on human leukemic hl-60 cells. | 2007 | 17854562 |
| development of species specific aflp-derived scar marker for authentication of panax japonicus c. a. meyer. | panax japonicus is an important medicinal plant. the aim of this study was to develop species-specific molecular markers for p. japonicus. amplified fragment length polymorphism (aflp) was compared among p. japonicus, p. ginseng and p. quinquefolius. a clear species-specific aflp marker for p. japonicus was generated. after isolation and sequencing of the aflp fragment, a dna sequence (293 bp) was obtained and named jg14. oligonucleotide primer (23 mer) was designed for amplifying 191 bp of the ... | 2008 | 18175956 |
| ultra-performance lc/tof ms analysis of medicinal panax herbs for metabolomic research. | in this study, metabolite profiling of five medicinal panax herbs including panax ginseng (chinese ginseng), panax notoginseng (sanchi), panax japonicus (rhizoma panacis majoris), panax quinquefolium l. (american ginseng), and p. ginseng (korean ginseng) were performed using ultra-performance lc-quadrupole tof ms (uplc-qtofms) and multivariate statistical analysis technique. principal component analysis (pca) of the analytical data showed that the five panax herbs could be separated into five di ... | 2008 | 18338405 |
| rapid detection of panax ginseng by loop-mediated isothermal amplification and its application to authentication of ginseng. | we have developed a novel method called loop-mediated isothermal amplification (lamp) to detect panax ginseng, the botanical source of ginseng (ginseng radix), and to distinguish p. ginseng from panax japonicus. six allele-specific primers (two outer primers, two inner primers, and two loop primers) were designed based on the 18s ribosomal rna gene sequence of p. ginseng, and lamp was performed using those primers and total dna extracted from p. ginseng as template. amplifications were observed ... | 2008 | 18758082 |
| immunological adjuvant effect of japanese ginseng saponins (jgs) on specific antibody and cellular response to ovalbumin and its haemolytic activities. | in this study, the saponins (jgs) extracted from the rhizoma of japanese ginseng were evaluated for their haemolytic activities and their potential ability as adjuvants on the immune responses to ovalbumin (ova) in mice. the haemolytic activity of jgs was determined using 0.5% rabbit red blood cell, with its hd(50) value being 177.78+/-6.77microg/ml. icr mice were immunized subcutaneously with ova 100microg alone or with ova 100microg dissolved in saline containing alum (200microg), quila (10 an ... | 2008 | 18801399 |
| [study on extraction process of polysaccharide constituents from panax japonicus by enzyme method]. | to choose the optimum extraction process of polysaccharide constituents from panax japonicus. | 2008 | 18837311 |
| [studies on preparative technology and quantitative determination for extracts of total saponin in roof of panax japonicus]. | to explore the optimum extraction and purification condition of the total saponins in the root of panax japonicus (rpj), and establish its quality control methods. | 2008 | 19216152 |
| a system for screening agonists targeting beta2-adrenoceptor from chinese medicinal herbs. | in order to develop a model for screening the agonists of human beta(2)-adrenoceptor from chinese medicinal herbs extracts, we used a cell-based functional assay based on a common g protein-coupled receptor (gpcr) regulation mechanism and destabilized enhanced green fluorescent protein (d(2)egfp) reporter gene technique. the positive cell clone was confirmed by real-time polymerase chain reaction (pcr) and imaging analysis. to assess the value of this model, we screened over 2000 high performanc ... | 2009 | 19353741 |
| panajaponin, a new glycosphingolipid from panax japonicus. | panajaponin, a new glycosphingolipid compound (1), together with eight known compounds, 28-glu-oleanolic acid ester (2), chikusetsusaponin iva (3), chikusetsusaponin iv (4), ginsenoside ro (5), ginsenoside re (6), notoginsenoside r2 (7), ginsenoside rg2 (8) and adenosine (9), was isolated from panax japonicus, and the structures of all of the compounds were established on the basis of nmr and ms spectra. | 2010 | 20013478 |
| potent alpha-glucosidase inhibitors from the roots of panax japonicus c. a. meyer var. major. | bioassay-guided fractionation of the chcl(3) soluble portion of the roots of panax japonicus c. a. meyer var. major afforded an active fraction with inhibitory activity against baker's yeast alpha-glucosidase with an ic(50) value 1.02 mg/ml. furthermore, the active fraction isolated contained three previously unreported polyacetylenes, designated panaxjapynes a-c, together with 11 other compounds, including four polyacetylenes, five phenolic compounds, a sesquiterpenoid, and a sterol glucoside. ... | 2010 | 20493502 |
| saponins from panax japonicus protect against alcohol-induced hepatic injury in mice by up-regulating the expression of gpx3, sod1 and sod3. | aims: the purpose of this study was to investigate the possible mechanism(s) of saponins from panax japonicus (spj) on alcohol-induced hepatic damage in mice. methods: spj were identified by high performance liquid chromatography-evaporative light scattering detection-mass spectrometry (lc-elsd-ms). non-toxic concentrations of spj were assayed on alcohol-induced hepatic injury in male icr mice and human hepatic cells. the protective effects were evaluated by biochemical values, histopathological ... | 2010 | 20554696 |
| [effect of panax japonicus on anti-oxidation of myocardial mitochondria of rats with high-intensity and endurance training]. | | 2010 | 20684280 |
| [culture of hairy roots of panax japonicus and ginsenoside re synthesis]. | to establish the induction method and culture system of hairy roots of panax japonicus, and determine ginsenoside re contents. | 2010 | 21141482 |
| [observation of anti-oxidation effect of total panax japonicus saponins on cerebral ischemia/reperfusion injury in rats]. | | 2008 | 21141524 |
| [effect of anti-oxidation function of panax japonicus saponins on mice' hypoxia/reoxygenation injury]. | | 2008 | 21141592 |
| [effect of total panax japonicus saponins on the contents of the excitatory amino acid in the brain tissue of rat with cerebral ischemia]. | | 2008 | 21158156 |
| bioactive constituents from the roots of panax japonicus var. major and development of a lc-ms/ms method for distinguishing between natural and artifactual compounds. | two new saponins, panajaponol (1) and pseudoginsenoside rt1 butyl ester (2), together with 35 known compounds (3-37), were isolated from the roots of panax japonicus var. major. the structures of 1 and 2 were elucidated on the basis of spectroscopic analysis and chemical methods. furthermore, a lc-ms/ms method was developed for confirming 2, 3, and 8 as natural compounds containing a butyl ester group. this method should be useful for distinguishing between minor natural and artifactual compound ... | 2011 | 21417387 |
| [effect of panax japonicus on activities of atpase of myocardial mitochondria from rats after exhausted exercise]. | | 2011 | 21560352 |
| [effects of polysaccharides isolated from panax japonicus on immunosuppression mice]. | to investigate the immunomodulatory effects of polysaccharides from panax japonicus on immunosuppressed mice. | 2011 | 21818977 |
| cardioprotective effects of saponins from panax japonicus on acute myocardial ischemia against oxidative stress-triggered damage and cardiac cell death in rats. | aim: to study the cardioprotective effects of saponins from panax japonicus (spj) on acute myocardial ischemia injury rats induced by ligating of the left anterior descending branch (lad), on the basis of this investigation, the possible mechanism of spj was elucidated. materials and methods: spj was identified by high performance liquid chromatography-evaporative light scattering detection. male sprague-dawley rats (200-220g) were randomly divided into four groups: sham-operated, lad, lad+l-spj ... | 2011 | 22226974 |
| [culture in vitro and plant regeneration of panax japonicus]. | to study the condition of culture in vitro and plant regeneration of panax japonicus. | 2011 | 22500414 |
| malonyl-ginsenoside rb1 in cell suspension culture of panax japonicus var. repens. | | 2011 | 22218959 |
| optimization of polysaccharides from panax japonicus c.a. meyer by rsm and its anti-oxidant activity. | response surface method (rsm) was used to optimize the extraction conditions of polysaccharides from the rhizomes of panax japonicus c.a. meyer. a three-level, three-variable box-behnken design (bbd) was applied for experimental design and analyzed of the results to obtain the optimal processing parameters. rsm analysis indicated good correspondence between experimental and predicted values. the optimal condition for the yield of polysaccharide was extraction time 3.74 h, extraction temperature ... | 2011 | 22214823 |
| saponins composition of rhizomes, taproots, and lateral roots of satsuma-ninjin (panax japonicus). | a new dammarane-type triterpenoid saponin, chikusetsusaponin vii (1), and nineteen known triterpenoid saponins, ginsenoside rb1 (2), ginsenoside rb3 (3), ginsenoside rc (4), ginsenoside rd (5), ginsenoside re (6), ginsenoside rg1 (7), ginsenoside rg2 (8), ginsenoside rh1 (9), notoginsenoside r1 (10), notoginsenoside r2 (11), notoginsenoside fe (12), chikusetsusaponin iva (13), chikusetsusaponin iv (14), chikusetsusaponin v (15), chikusetsusaponin vi (16), chikusetsusaponin fk6 (17), gypenoside x ... | 2012 | 23291557 |