| microsatellite isolation, linkage group identification and determination of recombination frequency in the peach-potato aphid, myzus persicae (sulzer) (hemiptera: aphididae). | fifteen polymorphic microsatellite markers were used to establish linkage groups and relative rates of recombination in male and female myzus persicae (sulzer) (hemiptera: aphididae) (peach-potato aphid). we cloned nine markers from m. persicae and for these we report primer sequences and levels of allelic diversity and heterozygosity in four australian m. persicae populations. of the remaining six loci, four loci, also cloned from m. persicae, were obtained from g. malarky (natural history muse ... | 2001 | 11486508 |
| a possibly new rickettsia-like genus symbiont is found in chinese wheat pest aphid, sitobion miscanthi (hemiptera: aphididae). | in this study, we investigated rickettsia infection in chinese wheat pest aphid (sitobion miscanthi), moreover detected a possibly new rickettsia-like symbiont, provisionally named as smls(1) (s. miscanthi l type symbiont). the sequence of smls 16s rrna gene is 94% similar to that of its presumed closest relative, orientia tsutsugamushi. if levels of divergence indicate taxonomic distinctiveness, smls probably represents a new genus in the family rickettsiaceae. smls occurs in most populations o ... | 2010 | 21156179 |
| cellular tropism, population dynamics, host range and taxonomic status of an aphid secondary symbiont, smls (sitobion miscanthi l type symbiont). | smls (sitobion miscanthi l type symbiont) is a newly reported aphid secondary symbiont. phylogenetic evidence from molecular markers indicates that smls belongs to the rickettsiaceae and has a sibling relationship with orientia tsutsugamushi. a comparative analysis of coxa nucleotide sequences further supports recognition of smls as a new genus in the rickettsiaceae. in situ hybridization reveals that smls is housed in both sheath cells and secondary bacteriocytes and it is also detected in aphi ... | 2011 | 21789197 |
| the genetic diversity of smls (sitobion miscanthi l type symbiont) and its effect on the fitness, mitochondrial dna diversity and buchnera aphidicola dynamic of wheat aphid, sitobion miscanthi (hemiptera: aphididae). | smls (sitobion miscanthi l type symbiont) is a recently discovered aphid secondary symbiont. using evidence extracted from 16s rrna sequences, previous studies indicate that smls is the most widely distributed and most recently transferred secondary symbiont in chinese sitobion miscanthi populations. here, we further investigated genetic diversity among smls geographic strains with multiloci data. furthermore, the influence of smls on s. miscanthi was uncovered with ecological and evolutionary e ... | 2016 | 27122251 |
| diversity of bacterial symbionts in populations of sitobion miscanthi (hemiptera: aphididae) in china. | aphids are a group of insects frequently associated with bacterial symbionts. although chinese aphids harbor a high level of species diversity, the associations between chinese aphids and bacterial symbionts are less known. in this study, we uncovered the diversity of bacterial symbionts in a chinese widespread aphid, sitobion miscanthi (takahashi). in this study, we detected the aphid obligate symbiont buchnera aphidicola, and two secondary symbionts, hamiltonella defensa and regiella insectico ... | 2014 | 24874152 |
| high spatial genetic structure and genetic diversity in chinese populations of sitobion miscanthi (hemiptera: aphididae). | the wheat aphid, sitobion miscanthi takahashi, a serious wheat pest, was previously considered to be highly migratory and anholocyclic in china. we recorded 69 alleles and 346 multilocus genotypes among 708 aphid individuals from 12 populations in china using 5 microsatellite loci. this genotypic diversity indicates that at least some holocyclic lineages exist. bayesian clustering analysis revealed that there are two differentiated genetic groups of s. misanthi, one northern and one southern, in ... | 2016 | 26487744 |
| microevolution, low clonal diversity and genetic affinities of parthenogenetic sitobion aphids in new zealand | in sharp contrast to their southeast asian and european counterparts, sitobion miscanthi and s. near fragariae aphids in australia exhibit a complete absence of sexual reproduction. this demands an explanation within the context of the evolution and maintenance of sex and parthenogenesis. accordingly, we executed a genetic analysis of the two species in neighbouring new zealand. microsatellites and single-stranded conformation polymorphism/sequence analysis of the nuclear gene elongation factor ... | 1999 | 10583829 |
| microsatellite and chromosome evolution of parthenogenetic sitobion aphids in australia. | single-locus microsatellite variation correlated perfectly with chromosome number in sitobion miscanthi aphids. the microsatellites were highly heterozygous, with up to 10 alleles per locus in this species. despite this considerable allelic variation, only seven different s. miscanthi genotypes were discovered in 555 individuals collected from a wide range of locations, hosts and sampling periods. relatedness between genotypes suggests only two successful colonizations of australia. there was no ... | 1996 | 8889535 |