| permanent light microscopy slides of eimeria nieschulzi oocysts. | | 1978 | 75264 |
| failure of nude (athymic) rats to become resistant to reinfection with the intestinal coccidian parasite eimeria nieschulzi or the nematode nippostrongylus brasiliensis. | the course of each of three successive infections with eimeria nieschulzi in nude (athymic) rats was the same as the primary infection in nu/+ animals, with the production of more oocysts. this indicates that resistance to reinfection with this parasite is mediated by t lymphocytes but that these cells do not control the duration of the life cycle, since oocyst production was not prolonged in the nu/nu rats. after the three infections with e. nieschulzi, the rats were exposed twice to the intest ... | 1979 | 551377 |
| suppressed rejection of trichinella spiralis in immunized rats concurrently infected with eimeria nieschulzi. | | 1978 | 627979 |
| intestinal disaccharidase and peroxidase deficiencies during eimeria nieschulzi infections in rats. | | 1978 | 690953 |
| intestinal transit time during infection with eimeria nieschulzi in rats. | | 1978 | 728258 |
| fine structure of the oocyst wall of eimeria nieschulzi. | oocysts of eimeria nieschulzi from the laboratory rat, rattus norvegicus, were studied by scanning and transmission electron microscopy. oocysts had a rough outer wall with apparent random depressions. the oocyst wall is composed of 2 layers: an osmiophilic outer layer consisting of a rough external and smooth internal surface, and a relatively thick, electron-lucent inner layer. the outer layer is composed of a dense, coarsely granular matrix. the inner layer consists of homogenous fine granula ... | 1978 | 739409 |
| [study of the oocyst of the coccidia eimeria nieschulzi by freeze-fracturing]. | ultrastructure of eimeria nieschulzi unsporulated oocyst is revealed by freeze etching. the two layers of the oocyst wall are observed; they are separated from the multilayered cytoplasmic pellicule by an intraoocystic fluid. nuclear envelope with pores, endoplasmic reticulum and mitochondrias surrounded by amylopectin granules are easy to identify. this technique makes possible the study of sporulation in eimeria, which cannot be done with the classical electron microscope methods. | 1975 | 807396 |
| external factors and self-regulating mechanisms which may influence the sporulation of oocysts of the rat coccidium, eimeria nieschulzi. | | 1977 | 858659 |
| the effects of heat and cobalt-60 gamma-radiation on excystation of the rat coccidium, eimeria nieschulzi dieben, 1924. | sporulated oocysts of eimeria nieschulzi dieben, a rat coccidium, were exposed for 1 hr to cobalt-60 gamma-radiation (15, 30, or 60k-rads), to heat (35, 40, or 45 c) , or to both concurrently (15, 30, or 60 k-rads at 35 c) to compared the excystation capabilities of treated vs nontreated parasites. intact treated oocysts appeared structurally unaltered when viewed with the light microscope. excystation of sporozoites occured in all treated groups when their sporocysts were exposed to a trypsin-s ... | 1977 | 864621 |
| the immunizing potential of sporulated oocysts of eimeria nieschulzi exposed to heat and co-60 gamma-radiation. | sporulated oocysts of eimeria nieschulzi dieben 1924, a rat coccidium, were exposed to radiation, heat, or both in an effort to attenuate the parasite. moderate levels of each treatment or combination thereof attenuated the parasite, reduced pathogenesis (as judged by oocyst discharge during primary infection), and produced immunity to challenge when the oocysts were subsequently inoculated into rats. thus, heat- and/or radiation-treated e. nieschulzi oocysts fed to rats could reduce pathogenesi ... | 1977 | 870663 |
| effect of select media supplements on motility and development of eimeria nieschulzi in vitro. | a technique was developed to examine the effects of exogenous substances on apicomplexan sporozoite motility in vitro. sporozoites of eimeria nieschulzi were placed in the top compartments of blind well chemotactic chambers and separated from potential chemoattractant/chemokinetic agents by 8.0-microns pore size millipore filters. after 3-hr incubations, the number of sporozoites migrating through the filters was assessed using a hemacytometer. results revealed that several substances, especiall ... | 1992 | 1372933 |
| development of a model ribosomal rna hybridization assay for the detection of sarcocystis and other coccidia. | two regions of the primary structure of the small subunit rrna of sarcocystis muris bradyzoites were compared with nucleotide sequences of s. gigantea, toxoplasma gondii, plasmodium berghei and mus musculus and used to design genus- and species-specific probes for the detection and identification of coccidia. total cellular rna of purified s. muris, s. cruzi, t. gondii and eimeria nieschulzi and coccidia-infected tissues of mouse, ox, sheep and pig, were assayed using twenty-base oligomers label ... | 1992 | 1423056 |
| effect of eimeria nieschulzi on nippostrongylus brasiliensis-induced ige. | | 1992 | 1557331 |
| identification of a 15-kilodalton surface glycoprotein on sporozoites of cryptosporidium parvum. | an immunoglobulin a monoclonal antibody (mab5c3) was developed against a 15-kda surface glycoprotein (gp15) of cryptosporidium parvum sporozoites. indirect immunofluorescence and colloidal gold immunoelectron microscopy revealed that the antibody reacted with both the sporozoite and merozoite surface plasma membranes. on western immunoblots, mab5c3 binding was found to be strongly inhibited when 200 mm n-acetylglucosamine was used as a competing sugar. n-acetylgalactosamine inhibited binding of ... | 1991 | 1705238 |
| novel ribonucleic acid species in eimeria nieschulzi are associated with rna-dependent rna polymerase activity. | sporulated oocysts of eimeria nieschulzi, e. tenella and e. acervulina were screened for the presence of putative viral nucleic acids. an rna-dependent rna polymerase activity in e. nieschulzi correlated with the presence of unknown nucleic acid species that were absent in the two other species of eimeria. the novel nucleic acid species also served as templates for rna-polymerase activity in in vitro synthesis of full-length labelled transcripts. these nucleic acid species were shown to be rnase ... | 1991 | 1792227 |
| fixing coccidian oocysts is not an adequate solution to the problem of preserving protozoan type material. | fresh (36 days old) sporulated oocysts of eimeria nieschulzi were divided into 7 groups. control oocysts were maintained at 23 c in 2% aqueous (w/v) k2cr2o7. the 6 experimental groups were mixed with either bouin's solution, 10% aqueous (v/v) buffered formalin, karnovsky's solution, glutaraldehyde, paraformaldehyde, or 70% aqueous (v/v) ethanol (etoh). after 115 days, oocysts from all 7 groups were examined under oil immersion to determine the effect of fixation on their structural integrity. th ... | 1991 | 1899451 |
| identification of sporozoite surface proteins and antigens of eimeria nieschulzi (apicomplexa). | sodium dodecyl sulfate polyacrylamide gel electrophoresis, immunoblotting, lectin binding, and 125i surface labeling of sporozoites were used to probe sporozoites of the rat coccidian, eimeria nieschulzi. analysis of silver stained gels revealed greater than 50 bands. surface iodination revealed about 14 well labeled, and about 10 weakly labeled but potential, surface proteins. the most heavily labeled surface proteins had molecular masses of 60, 53-54, 45, 28, 23-24, 17, 15, 14, 13, and 12 kd. ... | 1990 | 2319491 |
| anamnestic stimulus-specific myoelectric responses associated with intestinal immunity in the rat. | changes in small intestinal motility reflective of local anaphylaxis are hypothesized to be associated with the rapid, immune-mediated rejection of infective larvae of the nematode trichinella spiralis. this hypothesis was tested by comparing intestinal myoelectric activity in primarily and secondarily infected rats; the latter group represented immunized hosts, i.e., immunized by initial contact with the parasite. patterns of slow waves, action potentials, interdigestive migrating myoelectric c ... | 1986 | 2420191 |
| transfer of eimeria nieschulzi using extraintestinal tissue. | the issue of extraintestinal infection by eimeria nieschulzi in the rat was addressed by transferring various tissues from infected to uninfected rats by mouth. all 6 rats receiving liver, spleen, or small intestine from rats killed at 3 or 8 hr postinoculation (pi), and all 5 rats receiving spleen and small intestine from rats killed 8 days pi, showed infections. rats receiving tissues from rats killed at 8 days pi showed infections 24 hr later, indicating that fourth-generation merozoites were ... | 1989 | 2524557 |
| secretion of trials during gliding motility of eimeria nieschulzi (apicomplexa, coccidia) sporozoites visualized by a monoclonal antibody and immuno-gold-silver enhancement. | | 1989 | 2616569 |
| characterization of a surface antigen of eimeria nieschulzi (apicomplexa, eimeriidae) sporozoites. | a monoclonal antibody (mcab 3c3) reacting with a pellicular antigen of eimeria nieschulzi sporozoites has been selected among hybridomas produced against this organism by immunofluorescence assay. this antigen has been shown to be located on the zoite surface by immunofluorescence on living organisms. capping and shedding of antigen-monoclonal antibody immune complexes was observed upon incubation at 37 degrees c. on western immunoblotting, two polypeptides at 22 and 26 kda were recognized by mc ... | 1989 | 2657714 |
| isolation and partial characterization of rhoptries and micronemes from eimeria nieschulzi zoites (sporozoa, coccidia). | homogenization and subcellular fractionation of sporozoites of eimeria nieschulzi have allowed the recovery of highly enriched fractions of rhoptries and micronemes. the isolated organelles kept their in situ morphological characteristics. their apparent densities in sucrose solutions were approximately 1.18 g/cm3 for rhoptries and 1.14 g/cm3 for micronemes. when subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page), the microneme fraction showed two major polypeptide ... | 1989 | 2755931 |
| suppression of phytohemagglutinin induced splenocyte proliferation during concurrent infection with eimeria nieschulzi and nippostrongylus brasiliensis. | results suggest that infection with eimeria nieschulzi (protozoa) interferes with splenocyte proliferation induced by infection with nippostrongylus brasiliensis (nematoda). | 1989 | 2759211 |
| peroxidase and gamma-glutamyl transpeptidase activities during eimeria nieschulzi (apicomplexa) and/or nippostrongylus brasiliensis (nematoda) infections in the rat. | results suggest that malabsorption of amino acids which occurs during eimeria nieschulzi and nippostrongylus brasiliensis infections in rats is not due to impairment by intestinal inflammation of gamma-glutamyl transpeptidase activity. | 1986 | 2867928 |
| suppression of nippostrongylus brasiliensis (nematoda)-induced lysophospholipase activity and peripheral eosinophilia by eimeria nieschulzi (apicomplexa). | interspecific interactions between nippostrongylus brasiliensis and eimeria nieschulzi were studied by measuring fecal lysophospholipase (lyph) activity and relative numbers of peripheral eosinophils in rats singly or concurrently infected with one or both parasite species. three groups of 10 rats each were inoculated with 2 x 10(3) n. brasiliensis l3 larvae and/or 5 x 10(5) e. nieschulzi sporulated oocysts. groups 1 and 2 were infected with e. nieschulzi or n. brasiliensis, respectively. group ... | 1987 | 2957479 |
| a comparative study of the development of eimeria nieschulzi in vitro under aerobic and reducing conditions. | sporozoites of the rat coccidian, eimeria nieschulzi dieben, 1924 (apicomplexa: eimeriidae), were inoculated onto monolayers of normal rat kidney (nrk) fibroblasts and cultured either under aerobic (5% co2/95% air) or reducing (desiccator jars modified into candle jars) conditions in rpmi-1640 supplemented with 5% fetal bovine serum, sodium bicarbonate, and antibiotics. under aerobic conditions, first-generation meronts were observed at 2 days postinoculation (dpi) and, except for individual thi ... | 1988 | 3193326 |
| on the status of eimeria nieschulzi oocysts embedded in resin eleven years ago: a permanent method for preserving coccidian oocysts. | sporulated oocysts of eimeria nieschulzi that were fixed and mounted on glass slides in polymerized resin in 1976 are examined. size, shape, and integrity of oocysts and sporocysts are compared to similar observations we made in 1977 and reported in 1978 (journal of parasitology 64: 163-164). our conclusion is that the methods we reported on in 1978 provide one opportunity to produce permanent specimens of sporulated oocysts that could be made available for deposit in nationally accredited museu ... | 1988 | 3397838 |
| suppression of peripheral eosinophilia by the coccidium eimeria nieschulzi (apicomplexa: eimeriidae) in experimentally infected rats. | four groups of 60 rats each were used to examine interspecific interactions between eimeria nieschulzi and nippostrongylus brasiliensis. rats in group 1 served as uninoculated controls. group 2 rats were each injected subcutaneously with 2.0 x 10(3) l3 larvae of n. brasiliensis. group 3 rats were each inoculated per os with 2.5 x 10(5) sporulated oocysts of e. nieschulzi. rats in group 4 were first infected with 2.0 x 10(3) larvae of n. brasiliensis and, at 8 days postinoculation, with 2.5 x 10( ... | 1987 | 3585624 |
| small intestinal sucrase activity during experimental infections with nippostrongylus brasiliensis and/or eimeria nieschulzi in rats. | | 1986 | 3751235 |
| systemic release of mucosal mast cell protease during infection with the intestinal protozoal parasite, eimeria nieschulzi. studies in normal and nude rats. | the systemic secretion of rat mucosal mast cell protease (rmcpii), a major product of rat mucosal mast cells (mmc), was examined during primary infections with the protozoan parasite, eimeria nieschulzi in cfh/b, athymic (rnu/rnu) and euthymic (rnu/+) rats. release of rmcpii into the blood stream (2.9 micrograms/ml of serum) of normal rats occurred within 1 day after infection. this response developed 3-6 hours after inoculation with oocysts, was dose-dependent, and was found in both naive and i ... | 1985 | 3906517 |
| effects of restrictions in dietary protein and vitamin a on the responses of rats to infections by nippostrongylus brasiliensis (nematoda) and n. brasiliensis plus eimeria nieschulzi (coccidia). | | 1985 | 3934093 |
| glycine absorption from the small intestines of rats after secondary infections with eimeria nieschulzi. | a second (challenge) infection of eimeria nieschulzi in clinically immune rats did not produce weight gain depression but caused a decrease in the absorption of glycine from the ileum. the malabsorption due to challenge was equivalent to that caused by the primary infection which did cause weight loss. | 1986 | 3943599 |
| intestinal absorption studies with glycyl-proline, glycine and ethanol in rats infected with eimeria nieschulzi. | the absorption of glycyl-proline, glycine, and ethanol through the intestinal wall was studied in vitro by an everted sac technique in rats infected with the coccidial parasite eimeria nieschulzi which causes damage and atrophy to the intestinal villi. the absorption of the dipeptide and of the amino-acid was reduced through tissue from infected animals but the transport of ethanol was similar in both infected and uninfected rats. the replacement of sodium by potassium in the mucosal bathing flu ... | 1985 | 4010481 |
| endogenous development of eimeria nieschulzi: fourth generation merogony. | | 1985 | 4093823 |
| nucleic acid precursor incorporation by eimeria nieschulzi (protozoa: apicomplexa) and jejunal villus epithelium. | | 1974 | 4419595 |
| eimeria nieschulzi infections in inbred and outbread rats: infective dose, route of infection, and host resistance. | | 1973 | 4694950 |
| immunity to the protozoan parasite eimeria nieschulzi in inbred cd-f rats. | | 1973 | 4718863 |
| eimeria nieschulzi: glucose absorption in infected rats. | | 1973 | 4744842 |
| host and parasite interactions during single and concurrent infections with eimeria nieschulzi and e. separata in the rat. | | 1972 | 5061658 |
| the living, endogenous stages of the rat coccidium, eimeria nieschulzi. | | 1966 | 5338869 |
| fine structure of microgametocytes and macrogametes of eimeria nieschulzi. | | 1967 | 5604480 |
| fine structure of schizonts and merozoites of eimeria nieschulzi. | | 1968 | 5703439 |
| attempted transmission of the rat coccidium eimeria nieschulzi to mice. | | 1966 | 5969109 |
| fine structure of sporozoites of eimeria nieschulzi. | | 1967 | 6038031 |
| local and systemic effects on inflammation during eimeria nieschulzi infection. | rats infected with eimeria nieschulzi, a coccidium that inhabits intestinal epithelium, have a lower basal inflammatory state in their intestinal mucosa eight days postinoculation as reflected by a drop in mucosal peroxidase activity and a decrease in the number of granulocytes in the lamina propria. the reduction of systemic inflammation in infected rats was assessed from a reduction in the formation of granulation tissue around a sterile cotton string implanted under the abdominal skin of the ... | 1984 | 6088767 |
| coccidiosis: characterization of antibody responses to infection with eimeria nieschulzi. | the antibody responses of rats to infection with the intestinal intracellular protozoan parasite eimeria nieschulzi were examined by a sensitive radio-immunoassay with a soluble preparation of sporulated oocysts as antigen. specific antibodies of the igm, igg1, igg2a and igg2b isotypes were found in the blood circulation and iga antibodies were detected in the bile and in intestinal washings. the igm response was rapid, its peak was relatively brief and it was not recalled by the reinoculation o ... | 1984 | 6608083 |
| effects of low-level infections by coccidia and roundworms on the nutritional status of rats fed an adequate diet. | experiments were conducted on male, sprague-dawley rats of the same age fed a standard laboratory diet to determine the comparative effects of single and concurrent subclinical infections by coccidia (eimeria nieschulzi) and roundworms (nippostrongylus brasiliensis) on nutritional status and metabolism. all infections produced anorexia, but it was more prolonged and intense with the concurrent ones. feed:gain ratios were increased only by infections by nematodes alone, but average daily gains we ... | 1983 | 6674288 |
| the microwave oven: a novel means of decontaminating parasitological specimens and glassware. | this study was conducted to determine the effects of microwave radiation on developmental and infective stages of eimeria nieschulzi, strongyloides ratti, and taenia taeniaeformis. fecal samples and laboratory preparations containing these three parasites were subjected to microwave radiation for brief periods in a microwave oven, and then in vitro and/or in vivo assessments of viability were made for each organism and preparation. our results showed that microwave irradiation is extremely effec ... | 1983 | 6681839 |
| arrested development in eimeria nieschulzi (apicomplexa: eimeriina): results of single infections. | arrested development of eimeria nieschulzi in the rat was investigated by feeding tissues of infected rats to coccidia-free rats. intestinal tissue of 33 infected rats was fed on days 9, 10, 11, 14, 17, 21, and 28 pi to 43 uninfected recipients. eight recipients, which represent 24.2% of the donors, later showed infection. infections were retained for as long as 21 days after the initial infection. a prepatent period of 6 to 7 days in the recipients allowed the inference that the retained stage ... | 1984 | 6737171 |
| interspecific interactions between nippostrongylus brasiliensis and eimeria nieschulzi in the rat. | interspecific interactions between nippostrongylus brasiliensis and eimeria nieschulzi were studied by measuring parasite fecundity and patency in rats concurrently infected with both species. nine groups of five rats each were inoculated with either 4 x 10(3) n. brasiliensis larvae or 2.6 x 10(5) e. nieschulzi sporulated oocysts or both. groups 1 and 2 served as singly-infected controls. group 3 rats were infected simultaneously with both parasites. rats in groups 4 to 8 were infected first wit ... | 1983 | 6854476 |
| coccidiosis: t-lymphocyte-dependent effects of infection with eimeria nieschulzi in rats. | the intestinal pathology caused by infection with eimeria nieschulzi was investigated and comparisons were made between the effects in athymic nude (rnu/rnu) rats and their heterozygous (rnu/+) litter-mates. most of the changes noted, i.e. increase in gut weight, partial villous atrophy and increased numbers of mast, goblet and pyroninophilic cells were shown to be largely or wholely thymus dependent. the numbers of intraepithelial lymphocytes were decreased in both groups during the period of s ... | 1982 | 6983178 |
| intestinal absorption of beta-methyl-d-glucoside in rats infected with eimeria nieschulzi. | | 1982 | 7119995 |
| fine structure of zygotes and oocysts of eimeria nieschulzi. | mature macrogamonts were present in the small intestine of rats 5.5 to 7.5 days postinoculation with eimeria nieschulzi oocysts; oocysts were present at 6 to 7.5 days. types i and ii wall-forming bodies in macrogamonts began to undergo ultrastructural changes within zygotes to form the outer and inner layers of the oocyst wall. before and during oocyst wall formation a total of 5 membranes (m1-5) were formed at or near the surface of the zygote. the outer and inner oocyst wall layers formed betw ... | 1980 | 7194371 |
| glycylproline absorption in rats infected with eimeria nieschulzi. | | 1981 | 7215640 |
| fine structure of nuclear division and microgametogony of eimeria nieschulzi dieben, 1924. | nuclear division and microgametogony of eimeria nieschulzi were studied by transmission electron microscopy. all nuclear divisions occurred in close proximity to the gamont surface with four centrioles situated between the nuclear envelope and the gamont plasmalemma. during early nuclear divisions, each nucleus had a rod or hourglass-shaped nucleolus, indicating that the nucleolus probably pinched in two during nuclear division. nuclear divisions occurred by nearly centrally located intranuclear ... | 1981 | 7324546 |
| eimeria nieschulzi and trichinella spiralis: analysis of concurrent infection in the rat. | | 1980 | 7389855 |
| absorption of glycine and proline from the small intestine of rats infected with eimeria nieschulzi. | the absorption of glycine and proline through the jejunum and ileum of rats with an eimeria nieschulzi infection was impaired when the amino acids were presented to the mucosal surface as either a mixture or the dipeptide, glycyl-proline. | 1980 | 7398843 |
| aminopeptidase of eimeria nieschulzi physico-chemical properties and action of antimalarial drugs. | an aminopeptidase capable of degrading synthetic substrates and short peptides has been demonstrated in eimeria nieschulzi sporulated oocysts. physico-chemical properties and the classic activator and inhibitory effects have been determined. the inhibitory activity of classic antimalarial drugs has been demonstrated and the study of the inhibitory mechanism by chloroquine has been further studied. lastly, the comparison between the aminopeptidase of eimeria genus parasites and that of plasmodium ... | 1980 | 7458163 |
| cytokine and immunoglobulin subclass responses of rats to infection with eimeria nieschulzi. | siv rats infected with a high dose (50,000 oocysts) of eimeria nieschulzi displayed clinical symptoms of coccidiosis such as diarrhoea (days 6 and 7 post-primary infection) and weight loss (days 6-8 post-primary infection) and were completely immune to challenge with a similar dose. the ability of rats to produce tumour necrosis factor (tnf) in vivo was enhanced during the period of oocyst excretion in the primary infection but significant production of tnf did not occur after challenge infectio ... | 1995 | 7609990 |
| characterization of surface antigens of eimeria nieschulzi (sporozoa, eimeriidae) merozoites. | two surface antigens of eimeria nieschulzi merozoites were characterized by monoclonal antibodies (mcab) as follows: an antigen of 31 (reducing conditions), 25 and 31 kda (nonreducing conditions), by mcab a1f6; and an antigen of 52 and 30 kda (reducing conditions) or 42 kda (nonreducing conditions), by mcab h7. the localization of the antigens in fluorescence and electron micrographs is presented. despite being accessible to antibodies on live (nonpermeabilized) parasites, neither antigens were ... | 1995 | 7770429 |
| virus-like particles in eimeria nieschulzi are associated with multiple rna segments. | rna preparations from sporulated oocysts of eimeria nieschulzi were found to contain 2 double-stranded rna segments of 5.0 kb and 5.7 kb that were not present in other species of eimeria. treatment of crude lysates with rnase a revealed that in addition to these two segments, 3 other segments of 0.57 kb, 0.72 kb and 11.5 kb were protected from digestion, suggesting that they were enclosed within particles. virus-like particles with a diameter of approximately 39 nm were purified by caesium chlor ... | 1994 | 8008024 |
| the effect of bcg, zymosan and coxiella burnetti extract on eimeria infections. | infection of animals with species of eimeria induces a hyper-reactivity to endotoxin as manifest by a greatly increased capacity of infected animals to produce tnf in response to lps in vivo compared with uninfected animals. this finding indicates priming for hyperactivation of macrophages by eimeria infection and raises the possibility that non-specific triggering of macrophages by agents such as bacille calmette-guerin (bcg), zymosan or coxiella burnetti extract may be a simple means of contro ... | 1996 | 8872185 |
| diffusion of microinjected markers across the parasitophorous vacuole membrane in cells infected with eimeria nieschulzi (coccidia, apicomplexa). | cells infected with the intracellular parasite eimeria nieschulzi were microinjected with lucifer yellow (457 da), biocytin lucifer yellow (850 da) and dextranrhodamine (10,000 da). immediately after injection of a mixture of the markers into the host cell cytoplasm, a differential diffusion pattern was observed in trophozoites and schizonts. lucifer yellow and biotin lucifer yellow were seen to enter the parasitophorous vacuole, whereas the dextran was excluded. since these markers cannot perme ... | 1997 | 9211514 |
| a merozoite-specific 22-kda rhoptry protein of the coccidium eimeria nieschulzi (sporozoa, coccidia) is exocytosed in the parasitophorous vacuole upon host cell invasion. | a monoclonal antibody (mab d12a4) was used to follow the genesis and fate of rhoptries from early first-generation merogony through second-generation merozoites of the rat coccidium eimeria nieschulzi. the epitope recognized by mab d12a4 belongs to a 22-kda protein which can be localized first in developing meronts 25 h post-infection. early rhoptries appear as distinct granules in the cytoplasm of developing meronts and elongate into mature organelles before merozoite release. the 22-kda protei ... | 1998 | 9569094 |
| phylogenetic relationships among rodent eimeria species determined by plastid orf470 and nuclear 18s rdna sequences. | phylogenetic analyses for 10 rodent eimeria species from different host genera based on plastid orf470 and nuclear 18s rdna sequences were done to infer the evolutionary relationships of these rodent eimeria species and their correlation to morphology and host specificity. the phylogenies based on both data sets clearly grouped the 10 rodent eimeria species into two major lineages, which reflect more their morphological differences than host specificity. species in lineage a have spheroidal to s ... | 2001 | 11336753 |
| eosinopenia induced by anti-il-5 antibody, but not eimeria nieschulzi extract, increases susceptibility to experimental amebic abscess of the liver (eaal) in gerbils. | | 2002 | 12031642 |
| suppression of extraintestinal and intestinal nippostrongylus brasiliensis-induced eosinophilia by eimeria nieschulzi. | eosinophil responses in extraintestinal and intestinal tissues were examined in august and sprague-dawley rats infected with nippostrongylus brasiliensis or eimeria nieschulzi (or both), and in uninfected controls to test the hypothesis that e. nieschulzi suppresses the systemic n. brasiliensis-induced eosinophil response. caudal vein blood, femoral bone marrow, bronchoalveolar lavage fluid, peritoneal lavage fluid, and duodenal and jejunal samples were collected on day 8 postinfection (pi) with ... | 2006 | 17152935 |
| improved excystation protocol for eimeria nieschulzi (apikomplexa, coccidia). | large numbers of sporozoites are a crucial prerequisite for in vitro experiments with eimeria species. there are no protocols to obtain high amounts of vital purified sporozoites of eimeria nieschulzi; therefore, an improved excystation protocol is urgently needed. most excystation procedures for eimeria oocysts use a mechanical disruption method for the release of sporocysts, assuming that oocyst disruption of eimeria does not require enzymes (proteases). however, rodent eimeria oocysts are sus ... | 2008 | 18183423 |
| reporter gene expression in cell culture stages and oocysts of eimeria nieschulzi (coccidia, apicomplexa). | the rat parasite eimeria nieschulzi is a suitable model for transfection studies and was used as an additional model organism for the genus eimeria. we describe the transfection of this apicomplexan parasites and the cultivation of transformed stages in cell culture and in vivo. the beta-galactosidase or yellow fluorescent protein was expressed in all parasitic stages up to the second merozoite generation in vitro under control of the heterologous promoter region of eimeria tenella mic1 gene pre ... | 2009 | 18797926 |
| new insights into the excystation process and oocyst morphology of rodent eimeria species. | in this study, the mechanism of excystation of the rodent parasites eimeria nieschulzi, from rats, and eimeria falciformis, from mice, was investigated. in vitro, oocysts of both species are susceptible to the protease pepsin, and sporocysts and sporozoites can be excysted in a similar way. scanning electron microscopy (sem) revealed a collapse of the oocysts wall at both polar ends after pepsin treatment. this occurs without any visible damage of the outer wall. using fluorescence and transmiss ... | 2011 | 21498113 |
| improvement of ultrastructural preservation of eimeria oocysts by microwave-assisted chemical fixation or by high pressure freezing and freeze substitution. | fixation and preparation for electron microscopy of coccidian oocysts is a general problem. especially in sporulated oocysts, proper fixation and resin infiltration are hindered by the robust oocyst wall. conventional chemical fixation therefore leads to artefacts that obscure cellular details in the oocysts. in this study, sporulated oocysts of eimeria nieschulzi were subjected to different fixation and embedding protocols: conventional chemical fixation and embedding in spurr's resin, microwav ... | 2011 | 21764370 |
| chimeric fluorescent reporter as a tool for generation of transgenic eimeria (apicomplexa, coccidia) strains with stage specific reporter gene expression. | progress in transfection of eimeria sporozoites leads to transformed oocysts, however the output of mutants after passages in the host animals is low. further enrichment of transgenic oocysts was dependent on fluorescent activated cell sorting and could not be achieved by drug selection. in this study, we fused the toxoplasma gondii dhfr-tsm2m3 pyrimethamine resistance gene with the yellow fluorescent protein (yfp) encoding sequence to provide continuous pyrimethamine resistance and fluorescence ... | 2012 | 22449589 |
| two cowp-like cysteine rich proteins from eimeria nieschulzi (coccidia, apicomplexa) are expressed during sporulation and involved in the sporocyst wall formation. | the family of cysteine rich proteins of the oocyst wall (cowps) originally described in cryptosporidium can also be found in toxoplasma gondii (tgowps) localised to the oocyst wall as well. genome sequence analysis of eimeria suggests that these proteins may also exist in this genus and led us to the assumption that these proteins may also play a role in oocyst wall formation. | 2015 | 26209229 |
| development of eimeria nieschulzi (coccidia, apicomplexa) gamonts and oocysts in primary fetal rat cells. | the in vitro production of gametocytes and oocysts of the apicomplexan parasite genus eimeria is still a challenge in coccidiosis research. until today, an in vitro development of gametocytes or oocysts had only been shown in some eimeria species. for several mammalian eimeria species, partial developments could be achieved in different cell types, but a development up to gametocytes or oocysts is still lacking. this study compares several permanent cell lines with primary fetal cells of the bla ... | 2013 | 23862053 |
| insight into the ultrastructural organisation of sporulated oocysts of eimeria nieschulzi (coccidia, apicomplexa). | sporulated oocysts of eimeria contain four sporocysts with two sporozoites each and a sporocyst residuum. the developing sporozoites are protected by the sporocyst wall and the robust double-layered oocyst wall. because of problems with conventional fixatives, high-pressure freezing, followed by freeze substitution was used to achieve optimal ultrastructural preservation of oocysts, sporocysts and sporozoites. after embedding in epon®, ultrathin sections were examined by electron microscopy to s ... | 2012 | 22955498 |
| distribution and processing of eimeria nieschulzi owp13, a new protein of the cowp family. | eimeria species are veterinary important coccidian parasites and are transmitted between hosts via oocysts. the infectious sporozoites are protected by the oocyst and sporocyst wall. tyrosine-rich proteins are well-known components of the eimeria oocyst wall. recently, cysteine motif containing proteins (cowp family), as described in toxoplasma gondii and cryptosporidium spp., have also been characterized in eimeria. here, we identified a novel cowp related protein, enowp13, and tracked it via t ... | 2018 | 29315981 |
| identification and analysis of eimeria nieschulzi gametocyte genes reveal splicing events of gam genes and conserved motifs in the wall-forming proteins within the genus eimeria (coccidia, apicomplexa). | the genus eimeria (apicomplexa, coccidia) provides a wide range of different species with different hosts to study common and variable features within the genus and its species. a common characteristic of all known eimeria species is the oocyst, the infectious stage where its life cycle starts and ends. in our study, we utilized eimeria nieschulzi as a model organism. this rat-specific parasite has complex oocyst morphology and can be transfected and even cultivated in vitro up to the oocyst sta ... | 2017 | 29210668 |