| is902, an insertion element of the chronic-enteritis-causing mycobacterium avium subsp. silvaticum. | an insertion sequence element of mycobacterium avium subsp. silvaticum was isolated and its complete nucleotide sequence determined. is902 is 1470 bp in size and is repeated 10-12 times per genome. an open reading frame of 1200 bp was identified, encoding a protein product of mr 43932. this protein is highly similar to the predicted proteins of is900 of mycobacterium paratuberculosis, is116 of streptomyces clavuligerus and is110 of streptomyces coelicolor. is902 lacks terminal inverted repeats a ... | 1992 | 1348267 |
| numerical taxonomy of mycobactin-dependent mycobacteria, emended description of mycobacterium avium, and description of mycobacterium avium subsp. avium subsp. nov., mycobacterium avium subsp. paratuberculosis subsp. nov., and mycobacterium avium subsp. silvaticum subsp. nov. | we performed a numerical taxonomy analysis of 38 mycobacterium paratuberculosis and related mycobacterial strains, including wood pigeon mycobacteria; this analysis was based on 22 tests, which were selected for their potential discriminative value from a total of 51 tests studied and produced four well-defined clusters. cluster 1 contained the m. paratuberculosis strains, including two strains isolated from crohn's disease patients; cluster 2 contained mycobacterium avium and mycobacterium intr ... | 1990 | 2397193 |
| solid-phase hybridization capture of low-abundance target dna sequences: application to the polymerase chain reaction detection of mycobacterium paratuberculosis and mycobacterium avium subsp. silvaticum. | polymerase chain reaction (pcr) has been widely applied to the detection of microorganisms. overall sensitivity of pcr tests may be substantially reduced due to a large excess of nontarget dna and inhibitory substances in the sample. we used a 5'-biotinylated 513-bp probe from the 3' region of the is 900 element specific for mycobacterium paratuberculosis (mptb) to capture target mptb dna from crude sample dna extracts. captured target dna was separated using streptavidin-coated magnetic particl ... | 1995 | 7793635 |
| further studies on the gs element. a novel mycobacterial insertion sequence (is1612), inserted into an acetylase gene (mpa) in mycobacterium avium subsp. silvaticum but not in mycobacterium avium subsp. paratuberculosis. | we have recently described the gs element, found in mycobacterium avium subsp. paratuberculosis (map), mycobacterium avium subsp. silvaticum (mas) and some isolates of mycobacterium avium subsp. avium serotype 2 (maas2), which contains a set of genes of low gc% content, putatively associated with the biosynthesis, modification and transference of fucose to cell wall glycopeptidolipids. here we describe a further gene of low gc% content (mpa), within the gs element in map. mpa is a putative acety ... | 2000 | 11118730 |
| comparative genomic hybridizations reveal genetic regions within the mycobacterium avium complex that are divergent from mycobacterium avium subsp. paratuberculosis isolates. | mycobacterium avium subsp. paratuberculosis is genetically similar to other members of the mycobacterium avium complex (mac), some of which are nonpathogenic and widespread in the environment. we have utilized an m. avium subsp. paratuberculosis whole-genome microarray representing over 95% of the predicted coding sequences to examine the genetic conservation among 10 m. avium subsp. paratuberculosis isolates, two isolates each of mycobacterium avium subsp. silvaticum and mycobacterium avium sub ... | 2005 | 15774884 |
| molecular identification of mycobacterium avium subsp. silvaticum by duplex high-resolution melt analysis and subspecies-specific real-time pcr. | accurate identification of mycobacterial species and subspecies is essential to evaluate their significance and to perform epidemiological studies. the subspecies of mycobacterium avium have different attributes but coincide in their zoonotic potential. our knowledge about m. avium subsp. silvaticum is limited, since its identification is uncertain. mycobacterium avium subsp. avium and m. avium subsp. silvaticum can be discriminated from each other based only on phenotypic characteristics, as th ... | 2015 | 25740770 |
| mycobacterium paratuberculosis and mycobacterium avium subsp. silvaticum dna cannot be detected by pcr in crohn's disease tissue. | the etiology of crohn's disease remains unknown. a putative mycobacterial cause of the disease is still controversial. | 1998 | 9823555 |
| polymerase chain reaction detection of mycobacterium paratuberculosis and mycobacterium avium subsp silvaticum in long term cultures from crohn's disease and control tissues. | thirty one cultures were established in mg3 medium from the intestinal tissues of 29 patients, including 18 with crohn's disease, five with ulcerative colitis, and six non-inflammatory bowel disease controls. all cultures grew either acid fast bacilli or uncharacterized spheroplasts. pellets from these cultures were coded and assayed blind for m paratuberculosis and m avium subsp silvaticum using is900- and is902-pcr (polymerase chain reaction) assays, respectively. is900 and is902 are multicopy ... | 1992 | 1427373 |