effect of chilling and freezing on survival of leptospira interrogans serovar pomona in naturally infected pig kidneys. 1979292921
plate assay for detection of leptospira interrogans serovar pomona hemolysin.a plate assay which utilizes the addition of a blood agar overlay for detection of hemolysin by colonies of leptospira interrogans serovar pomona is described.1979528687
hazards of disease transfer from marine mammals to land mammals: review and recent a 5-year study (1972-1977) of microbial agents isolated from both clinically normal and diseased marine mammals, it was shown that certain disease agents are widespread in a diversity of ocean populations and that some are also transmissible to a number of terrestrial mammal species. leptospira interrogans serovar pomona has been isolated repeatedly from 2 species of pinnipeds (zalophus californianus califonianus and callorhinus ursinus). some of the more important bacterial pathogens for lan ...1978738931
susceptibility of mice treated with cyclophosphamide to lethal infection with leptospira interrogans serovar pomona.mice not normally susceptible to infection with leptospira interrogans serovar pomona were rendered susceptible to lethal infections by treatment with a single dose of 300 mg of cyclophosphamide (cy) per kg administered optimally from 4 days before to 1 day after infection. cy-treated mice with either passively or actively acquired antibody were protected from death. blood levels of leptospires in infected untreated and in cy-treated mice remained similar until 2 days after infection, when untre ...1976965093
specific immunofluorescent staining of pathogenic treponemes with a monoclonal antibody.two hybrid cell lines which produced mouse monoclonal antibody to the dal-1 street strain of treponema pallidum subsp. pallidum were established. these monoclonal antibodies strongly reacted with t. pallidum subsp. pallidum (nichols strain, dal-1, and two other street strains, strains mn-1 and mn-3) and t. pallidum subsp. pertenue by indirect microimmunofluorescent antibody and enzyme-linked immunosorbent assay techniques, but they did not react with normal rabbit testicular tissue. these monocl ...19921374079
characterization of the periplasmic flagellum proteins of leptospira interrogans.the structure and composition of periplasmic flagella (pf) from leptospira interrogans serovar pomona type kennewicki were characterized. electron microscopic observations showed that leptospiral pf were complex structures composed of an 11.3-nm-diameter core surrounded by two sheath layers with 21.5- and 42-nm diameters. two-dimensional gel electrophoresis of isolated pf showed the presence of seven different proteins ranging in mass from 31.5 to 36 kda. rabbit polyclonal and mouse monoclonal a ...19921624463
characterization of outer membrane and secreted proteins of leptospira interrogans serovar pomona.outer membrane and secreted proteins were isolated from leptospira interrogans serovar pomona and characterized by sodium dodecyl sulfate polyacrylamide gel electrophoresis, immunoblot and radioimmunoprecipitation techniques. the l. interrogans outer membranes were extracted with triton x-114 and contained several proteins. the major cellular protein with a molecular mass of 31 kda was associated exclusively with the l. interrogans outer membrane. using a whole cell immunoprecipitation method, f ...19911895930
expression of two conserved leptospiral antigens in escherichia coli.the genes encoding two protein antigens of leptospira interrogans serovar pomona were cloned and expressed in escherichia coli. rabbit antisera raised against the cloned proteins, designated p12 and p20, were used to identify the antigens in western blots of disrupted leptospiral cells. the proteins p12 and p20 were conserved within the genus leptospira and were not detected in leptonema illini. although both proteins were present in leptospiral outer envelope preparations they did not elicit th ...19892644433
copurification of leptospira interrogans serovar pomona hemolysin and sphingomyelinase c.the hemolytic and sphingomyelinase c activities of supernatants of cultures of leptospira interrogans serovar pomona tended to copurify when isoelectric fractionation was carried out. both activities focused primarily at ph 8.1. considered in conjunction with other circumstantial evidence, the results led to the conclusion that sphingomyelinase c is responsible for hemolysis.19863019890
prevention of leptospira interrogans serovar pomona infection in domestic pigs by vaccination. 19883190600
leptospirosis in red foxes in ontario.the role of the red fox (vulpes vulpes) in the epizootiology of leptospirosis in southwestern ontario was investigated in 1973-1974. leptospira interrogans serovar pomona (kennewicki by dna analysis) was isolated from the kidneys of three of eight foxes tested. severe hemorrhagic nephritis and interstitial nephritis were common to these foxes and to five others out of nine foxes examined. autumnalis antibodies were detected at titers 10(-2) to 10(-5) in 12 of 100 fox sera. pomona antibodies occu ...19863503132
the isolation of leptospira interrogans serovar pomona and related serological findings associated with a mixed farming unit in the transvaal.this is the first known isolation in the republic of south africa (rsa) of the serovar pomona from the organs of porcine foetuses as well as from the renal lymph nodes of slaughter pigs showing chronic nephritis. in addition, the serovar pomona was isolated from the kidneys of 87.5% of the slaughter pigs examined. the success of these isolations was attributed in part to the refining of 2 existing isolation techniques which complement each other. using the microscopic agglutination test, serum s ...19873627726
radiometric method for the rapid detection of leptospira organisms.a rapid and sensitive radiometric method for detection of leptospira interrogans serovar pomona and leptospira interrogans serovar copenhageni is described. stuart's medium and middlebrook tb (12a) medium supplemented with bovine serum albumin, catalase, and casein hydrolysate and labeled with 14c-fatty acids were used. the radioactivity was measured in a bactec 460 (johnston laboratories). with this system, leptospira organisms were detected in human blood in 2 to 5 days, a notably shorter time ...19863700624
morphological changes in red blood cells of calves caused by leptospira interrogans serovar pomona.haemoglobinaemia is seen in certain hosts infected by certain serovars of leptospira interrogans, but is absent from other serovar-host associations. comparisons were made between calves infected with serovar pomona and those receiving a crude "toxin" prepared from the same organism. red blood cells from "toxin"-injected calves showed discocyte-echinocyte transformation and contained portions of cytoplasm segregated within vacuoles. these animals showed increased sequestration of rbcs within the ...19863760264
pathogenesis and red blood cell destruction in haemoglobinaemic leptospirosis.sequential morphological changes were seen in rbcs, spleen and liver from hamsters during the development of haemoglobinaemia following infection with leptospira interrogans serovar ballum. spleens from pre-haemoglobinaemic hamsters showed sequestration of rbcs and erythrophagocytosis but to a lesser degree than was seen in the haemoglobinaemic hamsters. erythrophagocytosis and rbc sequestration were also seen in the liver, particularly in the haemoglobinaemic animals. none of these changes was ...19863760265
effects of products of autolysis of tissue and urine on the viability, morphology, and antigenicity of leptospira interrogans serovar pomona.the effect of the products of autolysis on leptospira interrogans serovar pomona was investigated in bovine kidney tissues and urine inoculated with this organism. no viable leptospires were found at 24 h or subsequently after inoculation of kidney tissues or urine. leptospires and their soluble antigens were rapidly destroyed in tissues stored at 20 degrees c and could not be detected by the fluorescent-antibody test and the enzyme-linked immunosorbent assay. on the other hand, leptospires were ...19853882747
enzymatic radioimmunoassay for detecting leptospira interrogans serovar pomona in the urine of experimentally-infected enzymatic radioimmunoassay (eria) has been developed for detecting leptospira interrogans serovar pomona in porcine urine. four grower pigs were experimentally infected with serovar pomona. a total of 39 urine samples was collected, and eria was compared with dark ground microscopy (dgm) and culture for demonstrating leptospiruria. of 20 samples positive by at least one technique, leptospires were detected by eria in 14, by culture in 16 and by dgm in 13. eria, unlike the other 2 methods, was ...19853890354
monoclonal antibodies to leptospira interrogans serovar pomona.three monoclonal antibodies produced against leptospira interrogans serovar pomona have been studied for their diagnostic usefulness. all three monoclonals reacted strongly in the enzyme-linked immunosorbent assay and indirect fluorescent antibody test with serovar pomona and did not react with serovars grippotyphosa, canicola, icterohaemorrhagiae and hardjo.19853893661
serological interrelationship of leptospira serovar and genus-specific antigens by enzyme-linked immunosorbent assay.the serological interrelationship of a sonicated antigen (pom-s) and an alkali-extracted "fraction 4" antigen (pom-f4) from leptospira interrogans serovar pomona, and an ethanol-precipitated (pat-e) and a formolized, sonicated antigen (pat-f) from leptospira biflexa serovar patoc were investigated. the serological responses of rabbits immunized with these antigens were examined by the enzyme-linked immunosorbent assay (elisa), the microscopic agglutination test, and the 2-mercaptoethanol microsc ...19846084016
maturation of humoral immune response determines the susceptibility of guinea-pigs to guinea-pigs were susceptible to infection with leptospira interrogans serovar pomona, but rapidly became resistant as they matured. increased resistance with increasing weight of guinea-pigs was correlated with the development of ability to produce agglutinating antibodies to leptospires and with maturation of b-cell dependent (but not t-cell dependent) areas in lymphoid organs. these observations can explain the basis of species-resistance of adult guinea-pigs to infection with serovar pom ...19806970358
evaluation of a hardjo-pomona vaccine to prevent leptospiruria in cattle exposed to a natural challenge with leptospira interrogans serovar hardjo.the efficacy of a vaccine against leptospira interrogans serovar pomona and leptospira interrogans serovar hardjo was evaluated in a group of dairy heifers that were serologically negative at the time of vaccination and later subjected to natural challenge with l. interogans serovar hardjo. thirty-nine heifers were vaccinated twice, at a one-month interval, with a commercially prepared bivalent vaccine, while 43 unvaccinated heifers were used as controls. after vaccination, microscopic agglutina ...19827115235
evidence that leptospiral lipopolysaccharide is not an important protective mice, which could not produce antibodies against lipopolysaccharide (lps) from either escherichia coli or leptospira interrogans serovar pomona, produced levels of agglutinating antibodies against leptospires similar to those produced by immunologically normal cba mice. cba/n mice were thus resistant to acute leptospiral infection and cba/n immune serum passively protected immunosuppressed mice from infection. the results suggest that antibodies against lps are not important in protection ...19827143431
immunogenicity of boiled compared with formalized leptospiral vaccines in rabbits, hamsters and humans.leptospires (leptospira interrogans serovar pomona) grown in chemically defined medium were immunogenic when given intradermally in humans if the leptospires were killed with formalin but not if they were boiled. boiled leptospires were immunogenic for rabbits and hamsters and protected hamsters from challenge infection. on the other hand, boiled leptospires of the biflexa complex, serovar patoc, did retain some immunogenicity in humans, but the antisera did not protect hamsters against challeng ...19807188698
characterization of an antigenic oligosaccharide from leptospira interrogans serovar pomona and its role in antigenic oligosaccharide fraction derived from the lipopolysaccharide of leptospira interrogans serovar pomona was isolated by endo-glycosidase h digestion and column chromatography. the oligosaccharide contained rhamnose, ribose, glucose, and glucosamine and inhibited the binding of opsonic, protective monoclonal antibodies directed against the lipopolysaccharide. when conjugated to diphtheria toxoid, the oligosaccharide elicited the production of agglutinating, opsonic antibodies.19947960129
comparative pathogenicity study of leptospira interrogans serovar pomona strains.the comparative pathogenicity study of two leptospira interrogans serovar pomona strains isolated from pig herds of different epizootiological status is reported. using monoclonal antibodies (mabs), the isolates were identified as leptospira interrogans serovar pomona. the results obtained for the reference strain of l. pomona were identical with those of the two isolates, with the exception of monoclonal serum designated 61-7, which gave a 1:30 titre with the reference strain. in a pig herd com ...19938017234
cloning and analysis of the leub gene of leptospira interrogans serovar pomona.the leub gene of leptospira interrogans serovar pomona strain kenniwicki has been cloned on a 9.5 kb plasmid, pwvl1, by complementation of escherichia coli leub mutants. subcloning and tn5 mutagenesis showed that the region required for complementation was approximately 1.2 kb in length. enzyme assays showed that the product of the cloned gene was a beta-isopropylmalate dehydrogenase. defects in the leua, leuc and leud genes of e. coli were not complemented by pwvl1. the nucleotide sequence of t ...19938336106
leptospiral abortion and leptospiruria in horses from the same farm.leptospirosis was documented as the cause of abortion in a 5-year-old mare. leptospires were detected in tissue specimens from fetal kidneys and from placenta by histologic evaluation of silver-stained sections. antibodies against leptospira interrogans serovar pomona were detected in fetal serum at a titer of 1,600 by use of a microscopic agglutination test. the mare had serum titers of 6,400; 0; 400; 800; 3,200; and 6,400 to l interrogans serovars bratislava, canicola, grippotyphosa, hardjo, i ...19938496088
preliminary evaluation of antimicrobial agents for treatment of leptospira interrogans serovar pomona infection in hamsters and evaluate antimicrobial agents for treatment of models of acute and persistent leptospirosis caused by leptospira interrogans serovar pomona.19968720239
the immunoglobulin response of swine following experimental infection with leptospira interrogans serovar pomona.the antibody response of pigs following experimental infection with leptospira interrogans serovar pomona was examined using enzyme immunoassay (eia) and the microscopic agglutination test (mat). leptospires elicited the production of both igm and igg classes of antibody, with igg levels persisting for much longer than igm. a comparison of mat and eia indicated that the detection of specific igm by eia was potentially useful in distinguishing between past and recent infection in pigs. agglutinin ...19846741345
restriction fragment length polymorphisms in australian and new zealand isolates of leptospira interrogans serovar pomona. 19968894026
identification of a 35-kilodalton serovar-cross-reactive flagellar protein, flab, from leptospira interrogans by n-terminal sequencing, gene cloning, and sequence analysis.during the screening of antibodies to pathogenic leptospires, a murine monoclonal antibody (designated m138) was found to react with various serovars. an antigen of approximately 35 kda from leptospira interrogans serovar pomona, which reacted strongly with m138, was characterized by n-terminal amino acid sequencing and identified as a flagellin, a class b polypeptide subunit (flab) of the periplasmic flagella. the gene encoding the flab protein, flab, was amplified from the genomic dna of sever ...19979317049
the first isolation of leptospira interrogans serovar pomona from cattle in botswana.serological evidence for the occurrence of l. pomona associated with abortions in cattle was supported by the successful isolation of the organism from the urine of a cow. leptospirosis should be considered as a possible cause of abortion even in relatively dry regions.19836631909
a pomona serogroup-specific, agglutinating antigen in leptospira, identified by monoclonal antibodies.monoclonal antibodies were produced by hybridoma cell lines derived by fusion of mouse ns-1 myeloma cells with splenocytes from mice immunized with leptospira interrogans serovar pomona. one hybridoma (a3) produced an igg2a antibody which agglutinated all leptospires of the pomona serogroup but not leptospires representative of serovars of any other serogroup. the antibody precipitated in immunodiffusions with either alkali- or phenol-extracted lipopolysaccharide and also with the tm antigen of ...19836417619
retinal immunopathology in horses with uveitis.equine uveitis is a spontaneous disorder of horses that can serve as a model for the study of human uveitis. although the initial presentation is that of an anterior uveitis, retinal involvement has been noted in some cases. we report here the immunohistopathology of retinas from horses with uveitis.19989924920
the enumeration of leptospira interrogans serovar pomona by a bioluminescence atp assay.a rapid method for enumerating viable leptospira interrogans serovar pomona cells was investigated using a bacterial adenosine triphosphate (atp) assay. the atp was assayed by the luciferin-luciferase bioluminescence reaction. samples of serovar pomona grown in liquid polysorbate 80-bovine albumin (p80-ba) medium for 1-3 days were analysed for atp content, culture density (nephelometry), direct cell count and most probable number of viable cells (mpnvc) as determined by the dilution tube techniq ...19863957938
isolation of a spirochete from the soft tick, ornithodoros coriaceus: a possible agent of epizootic bovine abortion.a borrelia-like spirochete was detected in all three parasitic stages of ornithodoros coriaceus, the soft tick implicated in the epizoology of epizootic bovine abortion. after the spirochete had been isolated, its distinctness from other north american tick-borne borreliae as well as from spirochaeta aurantia, treponema pallidum, and leptospira interrogans serovar pomona was established on the basis of its morphology, protein components, and inability to infect mice. the spirochete is passed tra ...19853898367
serologic correlation of suspected leptospira interrogans serovar pomona-induced uveitis in a group of horses.after the observation of 2 horses with uveitis on a horse farm in the minnesota river valley, 100 horses from this geographic area were given ophthalmologic examinations and were evaluated serologically for leptospirosis. a statistically significant (p less than 0.001) association was observed between the finding of antibodies against leptospira interrogans serovar pomona and uveitis.19873693011
a dna fragment of leptospira interrogans encodes a protein which shares epitopes with equine cornea.horses infected with leptospira interrogans present several clinical disorders, one of them being recurrent uveitis. an antigenic relationship between this bacterium and equine cornea has been described in previous studies. with the aim to make progress on defining the molecular basis and pathogenesis of equine recurrent uveitis, here we describe the cloning of one dna fragment from a leptospira interrogans serovar pomona genomic lambda gt11 library. although there are references of transcriptio ...199910587299
[incidence of leptospiroses caused by leptospira interrogans serovar pomona in east germany]. 19863492825
development of an indirect enzyme-linked immunosorbent assay for the detection of leptospiral antibodies in dogs.serology plays an important role in the diagnosis of leptospirosis. few laboratories have the resources, expertise, or facilities to perform the microscopic agglutination test (mat). thus, there is a need for a rapid and simple serological test that could be used in any diagnostic laboratory. in this study, a genus-specific, heat-stable antigenic preparation from leptospira interrogans serovar pomona was used in an enzyme-linked immunosorbent assay (elisa) for the detection of leptospiral antibo ...200010680654
effect of maternal vaccination on the susceptibility of growing pigs to leptospiral infection.serum samples were collected from 30 piglets, derived from 17 litters, whose dams had been vaccinated against leptospirosis. microscopic agglutination test (mat) titres against leptospira interrogans serovar pomona varied greatly from pig to pig; there was less variation among littermates. titres declined between 4 and 10 weeks of age, with an uncorrected half-life of 15.5 days, consistent with igg being the main antibody class involved. twelve pigs, 4 derived from unvaccinated sows and 8 from s ...19873439018
antibody isotypes in sera of equine fetuses aborted due to leptospira interrogans serovar pomona-type kennewicki infection.leptospira-specific antibody isotypes in sera of late term equine fetuses aborted due to leptospira interrogans serovar pomona-type kennewicki infection were characterized and compared with those of their dams. igm was the dominant leptospira-specific isotype in both fetuses and mares. however, igga was the isotype in highest concentration in petal sera and strong leptospira-specific igga but no iggb and little or no igg(t) were detected. in contrast, although iggb was quantitatively the dominan ...200011137127
detection of leptospires in biological fluids using dna hybridisation.dna extracted from leptospira interrogans serovar pomona was labelled with phosphorus-32 by nick translation and used as a genomic probe to detect leptospiral dna. the sensitivity of detection in a 10-microliter spot on nylon membranes was 160 pg of leptospiral dna or 1.1 x 10(3) leptospires and assays with nylon membranes were somewhat more sensitive than assays with nitrocellulose membranes. the probe reacted with the pathogenic hardjo and tarassovi leptospiral serovars, but not with other gen ...19873439017
[leptospira interrogans serovar pomona: detection of antigenic differences among 3 regional isolates from cattle and a reference strain].leptospirosis is a zoonosis caused by leptospira interrogans. this disease is diagnosed by quantification of specific immunoglobulins in serum by the microagglutination test (mat). the aims of this research were: a) to compare the protein profiles of 3 clinical isolates of bovine leptospirosis with the reference strain used for the mat, and b) to identify the immunodomain antigens of the regional isolates through page and immunoblotting techniques of bovine sera from infected, vaccinated and mat ...200111494753
monoclonal antibodies reacting with serogroup and serovar specific epitopes on different lipopolysaccharide subunits of leptospira interrogans serovar pomona.monoclonal antibodies with two kinds of specificities, produced against leptospira interrogans serovar pomona, were studied by agglutination and immunoblotting. antibodies reacted either exclusively with serovar pomona or with all members of the pomona serogroup, but none of the antibodies reacted with representative serovars of other serogroups. both antibodies recognized epitopes on purified lipopolysaccharide (lps) from serovar pomona. in immunoblotting experiments the serogroup specific anti ...19892483523
antibody response to genus- and serovar-specific leptospiral antigens in leptospira-infected cows inoculated with leptospira interrogans serovar pomona or hardjo, the 2-mercaptoethanol-sensitive microscopic agglutination test (mat) antibody to the serovar appeared 3 to 8 days after inoculation and peaked at 10 to 20 days, whereas the 2-mercaptoethanol-resistant mat antibody was predominant at 35 to 80 days. a persistent antibody response, probably associated with serovar-specific leptospiral antigens, was detected in the cows inoculated with serovar pomona, using a sonicated or an al ...19852411175
a multiplex pcr for the detection of brucella spp. and leptospira spp. dna from aborted bovine fetuses.bovine brucellosis and leptospirosis are important causes of bovine abortion around the world. both diseases can be serologically diagnosed, but many factors may cause false positive and negative results. direct methods based on bacteriological isolation are usually employed, but they are difficult, time consuming and dangerous. monoplex polymerase chain reaction (pcr) have been successfully described for the detection of brucella spp. and leptospira spp. aiming at improvement in the direct diag ...200212034542
genetic diversity among australian and new zealand isolates of leptospira interrogans serovar pomona.restriction endonuclease analysis of 16 australian and 4 new zealand isolates of leptospira interrogans serovar pomona showed that they could be divided into 3 genetic groups. most of the isolates closely resembled the serovar kennewicki reference strain, and they all differed from the reference strain of serovar pomona. based on these findings, it is suggested that vaccine manufacturers re-evaluate their choice of serovar pomona vaccine strain.19921632725
cloning and molecular characterization of an immunogenic liga protein of leptospira interrogans.a clone expressing a novel immunoreactive leptospiral immunoglobulin-like protein a of 130 kda (liga) from leptospira interrogans serovar pomona type kennewicki was isolated by screening a genomic dna library with serum from a mare that had recently aborted due to leptospiral infection. liga is encoded by an open reading frame of 3,675 bp, and the deduced amino acid sequence consists of a series of 90-amino-acid tandem repeats. a search of the ncbi database found that homology of the liga repeat ...200212379666
assay for measuring relative potency of leptospiral bacterins containing serovar enzyme-linked immunosorbent assay (elisa) was developed for the quantitation of leptospiral antigen in bacterins containing leptospira interrogans serovar pomona type kennewicki. a monoclonal antibody (mab), 2d7, which is directed against a surface antigen on whole cells of l. interrogans serovar pomona type kennewicki, was used in the assay. the capture of antigen in bacterins by a polyclonal antiserum was followed by the addition of the 2d7 ascites fluid, an anti-mouse conjugate and substra ...19921305402
leptospira interrogans serovar pomona vaccines with different adjuvants in cattle. 1979388262
presence of vi antigen in a virulent strain of leptospira interrogans serovar pomona and relation of vi antigens of leptospiras to resistance to leptospiricidal activity mediated by antiserum plus antigen was found in a virulent strain of leptospira interrogans serovar pomona. the presence of vi antigen was shown by the agglutinin-absorption and precipitin-absorption tests. each strain of pomona and copenhageni which possessed vi antigen and was resistant to the leptospiricidal activity mediated by the antiserum plus complement lost vi antigen and became susceptible to the leptospiricidal activity when cultured in the presence of 8-azaguanine. the strains thus treated with 8-azaguanine ...19827180239
development of a polymerase chain reaction test to confirm mycobacterium avium subsp. paratuberculosis in culture.a polymerase chain reaction (pcr) assay for confirmation of mycobacterium avium subsp. paratuberculosis was developed using the primer set derived from ismav2. the pcr product was 494 base pairs (bp) and could be digested with clai, which produced 311- and 183-bp fragments. no amplification of 494-bp dna fragment was detected from dna of other mycobacterium spp., including mycobacterium avium complex, other bacteria, including escherichia coli, pseudomonas aeruginosa, actinobacillus pleuropneumo ...200415053361
leptospira interrogans serovar pomona associated with abortion in cattle: isolation methods and laboratory animal histopathology.leptospira interrogans serovar pomona was successfully isolated from cattle urine in the western transvaal after an abortion storm had occurred. direct inoculation of emjh medium proved the most successful method. the selective agent, 5-fluorouracil, was most effective in controlling contamination when used at the 0,4 mg/ml level. the strain isolated was pathogenic in hamsters, but specific lesions and the leptospirae were seen only where overwhelming infection occurred.19827122066
studies on equine recurrent uveitis. ii: the role of infection with leptospira interrogans serovar enzyme linked immunosorbent assay was developed for the detection of immunoglobulin class specific antibodies to leptospira interrogans serovar pomona in the serum and aqueous humor of horses. serum antibody was also assayed by microscopic agglutination tests. although higher levels of antibody were found in sera from horses with signs of uveitis, the association was not statistically significant. antibodies to pomona were detected in the aqueous of 12 eyes from the 101 horses sampled at a sl ...19853877614
surface colonies of leptospira interrogans.observations of colonial growth of leptospira interrogans serovar pomona, bj, on soft-agar (1% agar) plates revealed the presence of both surface and subsurface colonies. the organisms from both types of colonies appeared to be similar in motility, morphology, antigenic composition, and pathogenicity. passage in vivo tended to produce a higher incidence of surface colonies, whereas passage in vitro tended to produce a higher incidence of subsurface colonies, suggesting the importance of environm ...19817462407
pathogenesis of renal lesions in haemoglobinaemic and non-haemoglobinaemic leptospirosis.hamsters were infected with leptospira interrogans serovar ballum or leptospira interrogans serovar pomona and the kidney lesions were compared by light and electron microscopy. ballum and pomona both caused severe clinical signs and death within 6 days in some animals, although only ballum was associated with red blood cell destruction and haemoglobinaemic nephrosis. with ballum infections it is difficult to distinguish degenerate changes resulting from leptospiral "toxins" from those resulting ...19892794156
leptospirosis associated with serovars hardjo and pomona in red deer calves (cervus elaphus).four red deer calves (cervus elaphus) died with severe nephritis apparently associated with infection by leptospira interrogans serovar pomona. the sera of 12 in-contact red deer calves were examined for leptospiral agglutinins and nine showed titres to pomona consistent with recent infection. two also showed titres of 1:100 to serovar hardjo. the urine of five of these in-contact calves was examined periodically over a period of nine months. all were initially leptospiruric, four being infected ...198416031052
a serological study of bovine leptospirosis in the district of taranaki.a cross-sectional serological survey of dairy cattle in taranaki in 1979-80 indicated that 62% (551/891) of the animals had evidence of leptospira interrogans serovar hardjo infection as disclosed by the microscopic agglutination test. titres to leptospira interrogans serovar pomona were demonstrated in only 4% (23/591) of the animals examined. the high prevalence of hardjo infection is suggestive of an endemic infection whilst the low level to pomona is indicative of sporadic infection. in a de ...198416031079
vaccination of mice with lipopolysaccharide (lps) and lps-derived immuno-conjugates from leptospira interrogans.mice were vaccinated with lipopolysaccharide (lps) from leptospira interrogans serovar pomona or hardjo, or with the polysaccharide (ps) fraction of the lps, or with an immunoconjugate of ps and diphtheria toxoid (dt). maximum agglutinin titres were found 6-10 weeks after vaccination with lps or ps-dt conjugate; the latter elicited antibody titres at least 10 times higher than those produced in response to lps. animals failed to react significantly to ps. titres elicited by antigens of serovar p ...19902250290
observations on three outbreaks of leptospira interrogans serovar pomona infection in lambs.sickness and deaths associated with leptospirosis were reported in three lamb flocks in the manawatu region. the clinical, pathological and serological features of leptospiru interrogans serovar pomona infection are described. the mucous membranes of affected animals were pale and jaundiced. haemoglobinuria, haemoglobinaemia and centrilobular hepatocellular necrosis were also consistent findings. all clinically sick lambs had very high titres in the microscopic agglutination test for l. pomona. ...199416031763
renal dysfunction associated with infection of leptospira interrogans in a horse.renal failure associated with infection of leptospira interrogans was detected in a horse. fever, leukocytosis, pyuria, isosthenuria, and azotemia were suggestive of an inflammatory urinary tract disease. despite persistent pyuria, no bacteria were found during routine microscopic examinations or bacteriologic culturing of urine. a fluorescent antibody examination of the urine was positive for l interrogans. serologic testing during a 6-month period, supported an acute infection with l interroga ...19921429185
survival of leptospira interrogans serovar pomona in an acidic soil under simulated new zealand field conditions.leptospira interrogans serovar pomona was found to survive for at least 42 days in a typical new zealand soil under simulated winter field conditions. the soil was markedly acidic with a ph of 5.5 and survival times were not reduced even when its water content was only 23%. the values of both these parameters are considerably less than previously recorded for the survival of leptospires in soil. two methods were used to recover leptospires from the soil microflora. one was the culture of a membr ...197830123
serologic responses of dogs given a commercial vaccine against leptospira interrogans serovar pomona and leptospira kirschneri serovar evaluate serum titers obtained by use of the microscopic agglutination test (ie, mat titers) to leptospira interrogans serovar pomona and autumnalis and leptospira kirschneri serovar grippotyphosa in dogs given a commercial vaccine against serovars pomona and grippotyphosa.200516273911
experimental canine leptospirosis caused by leptospira interrogans serovars pomona and evaluate gross, histopathologic, and serum biochemical findings caused by leptospira interrogans serovars pomona and bratislava inoculated in dogs.200516273916
immunoprotection of recombinant leptospiral immunoglobulin-like protein a against leptospira interrogans serovar pomona infection.we previously reported the cloning and characterization of leptospiral immunoglobulin-like proteins liga and ligb of leptospira interrogans. liga and ligb are conserved at the amino-terminal region but are variable at the carboxyl-terminal region. here, we evaluate the potential of recombinant liga (rliga) as a vaccine candidate against infection by l. interrogans serovar pomona in a hamster model. rliga was truncated into conserved (rligacon) and variable (rligavar) regions and expressed in esc ...200616495547
correlation between dna restriction fragment length polymorphisms in leptospira interrogans serovar pomona type kennewicki and host animal source.isolates (n = 147) of leptospira interrogans serovar pomona type kennewicki from cattle, swine, horses, and wildlife were analyzed by dna restriction endonuclease analysis. restriction fragment length polymorphisms were identified in dna digested with hpaii, and the restriction fragment length polymorphisms were correlated with the host animal source of the isolates. these results will be useful in understanding the epidemiology of serovar pomona infections in livestock.19968789028
leptospirosis: an occupational hazard to veterinarians.leptospirosis occurred in two veterinarians in alberta, following their exposure to leptospires of domestic animal origin. the disease at onset resembled "flu" with fever, muscle and joint pain, and lassitude. it progressed through an extremely debilitating period with mild to severe hepatic and renal dysfunction, icterus and hemorrhage in one case, and cerebral meningitis in the other. both patients were hospitalized for 11 to 14 days, where they responded to supportive and specific antibiotic ...198617422627
leptospirosis outbreak in a piggery in southern alberta.fourteen abortions, stillbirths and neonatal deaths occurred over an interval of one month in crated sows in a farrow-to-finish swine operation. leptospira interrogans serovar pomona (kennewicki by dna analysis) was demonstrated by culture and fluorescent antibody test to be present in sows, piglets, boars, feeders, drinking water and skunks on the premises.antibody was found in all clinically affected sows, at serum dilutions ranging from 1/800 to 1/25,600, and in all breeding boars at titers f ...198617422651
association of leptospiral seroreactivity and breed with uveitis and blindness in horses: 372 cases (1986-1993).recurrent uveitis, a leading cause of blindness in horses, often develops as a sequela to systemic leptospirosis. over a 7-year period, 63 of 112 (56%) horses with uveitis were seropositive for leptospira interrogans serovar pomona, but only 23 of 260 (9%) horses without uveitis were seropositive. odds-ratio analysis revealed that seropositive horses were 13.2 times more likely to have uveitis than were seronegative horses. of the 63 seropositive horses with uveitis, 59% developed blindness, com ...19957591929
use of recombinant flagellin protein as a tracer antigen in a fluorescence polarization assay for diagnosis of leptospirosis.the objective of the present study was to investigate the usefulness of a recombinant flagellar protein, flab, of leptospira interrogans serovar pomona in the serodiagnosis of leptospirosis by the fluorescence polarization assay (fpa). the recombinant protein flab was purified to homogeneity by a combination of nickel-nitriloacetic acid agarose chromatography, electrophoresis, and electroelution. purified flab was labeled with fluorescein isothiocyanate (fitc). western blotting was performed by ...199910391870
leptospirosis in farmed deer in new zealand : a review.current knowledge of leptospirosis in farmed deer in new zealand is reviewed. over the past 25 years, leptospirosis has been reported to occur in individual cases as well as in herd outbreaks in farmed deer and in human cases linked to farmed deer. serological studies and evidence from bacterial culture suggest infection is widespread. mixing of young stock from several sources appears to be a significant risk factor for outbreaks. the culture of leptospira interrogans serovars hardjobovis, pomo ...200717534410
diagnosis and prevalence of leptospira infection in aborted and stillborn horses.a study was conducted to evaluate a recently available fluorescent antibody test (fat) conjugate for the detection of leptospires in tissues of aborted and stillborn horses, to determine the leptospira antibody titers and compare serologic test results with fat results, and to determine the prevalence of leptospira-induced abortions and stillbirths in the equine population of central kentucky. from july 1, 1988 through june 30, 1989, 15 (2.5%) of 594 submissions (fetuses, stillborn foals, and/or ...19911892931
leptospirosa pomona abortion storm in a cattle herd in saskatchewan.abortions occurred in 18% of 131 beef cows and heifers during two months, on a farm in southern saskatchewan. the losses began two weeks after acute febrile illness and agalactia in a dairy cow to which the beef herd had been exposed. a diagnosis of leptospira interrogans serovar pomona infection was made on the basis of serology in cows and the finding of leptospires in fetal tissues by fluorescent antibody test. tentative diagnosis of infectious bovine rhinotracheitis delayed treatment and pro ...198617422717
multiple-locus variable-number tandem repeat analysis (mlva) of leptospira interrogans serovar pomona from argentina reveals four new genotypes.outbreaks of leptospirosis occur regularly in argentina, but little is known about their epidemiological relationships. we have analyzed the genetic diversity of a collection of 16 strains of leptospira interrogans serovar pomona isolated from animals and humans in argentina during the past 45 years. genotyping was performed by multiple-locus variable-number tandem repeat analysis (mlva) using the loci vntr4, vntr7, vntr9, vntr10, vntr19, vntr23 and vntr31, as described by majed et al. [identifi ...200817531318
serological survey for leptospires of agricultural importance in common wombats (vombatus ursinus). 200415119734
characterization of is1501 mutants of leptospira interrogans serovar pomona.leptospira interrogans is a diverse species in which individual serovars have distinctive restriction fragment length polymorphisms that are useful in strain identification. many of these polymorphisms can be detected using hybridization probes derived from insertion sequences; an observation that suggests these is elements are active and can transpose in l. interrogans. two spontaneous mutants of l. interrogans serovar pomona strain rz11 were isolated by immune selection and characterized. chan ...200515979822
risk factors associated with the likelihood of leptospiral seropositivity in horses in the state of new determine and quantify risk factors associated with exposure of horses to the following serovars of leptospira interrogans: pomona, autumnalis, and bratislava.19979328661
[identification of leptospira interrogans serovar pomona in mice genetically selected for high and low antibody production, by means of the immunoperoxidase technique in renal tissue and bacterial isolation in fletcher medium].the present work had the objective of identifying the presence of leptospira interrogans serovar pomona in mice that had been genetically selected for high and low response to antibodies. all the animals were subjected to bacterial isolation, immunohistochemical analysis (immunoperoxidase) in renal tissue sections and hematoxylin-eosin staining. the immunoperoxidase technique was little more sensitive than culturing, but both were good parameters for agent identification. more severe renal lesio ...200718200418
prevalence and geographic origin of pigs with serological evidence of infection with leptospira interrogans serovar pomona slaughtered in abattoirs in victoria, australia.a set of 10,440 sera was collected from pigs slaughtered at victorian abattoirs. these sera were subjected to the microscopic agglutination test for antibodies to leptospira interrogans serovar pomona. identification of the herd of origin was possible for 6511 pigs, and these were derived from 167 herds in victoria (84% of sera), from 32 herds in new south wales (8% of sera) and 29 herds in south australia (8% of sera). the overall prevalence of titres of 512 and above was 3.7%. this was higher ...19989791870
three cases of canine leptospirosis in quebec.three dogs from different locations with acute renal failure were hospitalized in autumn 1996 and 1997. leptospira interrogans serovar pomona was detected by the microscopic agglutination test. all dogs recovered after antibiotic treatment. the importance of the development of vaccines adapted to emerging serovars in dogs should be addressed.199910086220
species- and genus-specific antigens in leptospira, revealed by monoclonal antibodies and enzyme immunoassay.mouse antisera to leptospira interrogans serovar pomona or l. biflexa serovar patoc agglutinated homologous leptospires but not leptospires of serovars from any heterologous serogroup tested. when tested by enzyme immunoassay (eia), pomona and patoc antisera reacted with all serovars tested from l. interrogans, l. biflexa or l. illini species. a non-agglutinating hybridoma-derived monoclonal antibody reacted by eia only with serovars of the pathogenic l. interrogans species but not with l. bifle ...19836359778
[development of a method to determine the minimum inhibitory concentration in reference strains from leptospiras].a method to determine the minimum inhibitory concentration for leptospiras was developed, since there is not a standard method to measure it at the international level. reference strains from the pathogenic complex l. interrogans and l. biflexa were used against penicillin, cyprofloxacine, chloramphenicol, rifampicine and tetracycline. the minimum inhibitory concentration was defined as the lowest concentration of antibiotic where it was observed the inhibition of the bacterial mobility by direc ...200517966469
expression in escherichia coli of flab, the gene coding for a periplasmic flagellin of leptospira interrogans serovar pomona.a periplasmic flagellin gene, flab, of leptospira interrogans serovar pomona (strain pomona) was expressed in escherichia coli for the production and antigenic characterisation of the protein. the flab structural gene, which was previously cloned into puc118, was derived by pcr from the recombinant plasmid and used to generate an expression construct with the trc promoter-driven pproex ht system. under the conditions employed, the flab was expressed as inclusion bodies formed within e. coli, yie ...199910535640
prevention of leptospira interrogans serovar pomona infection in cattle.a commercial hardjo-pomona vaccine which has previously been shown to be effective against hardjo infection was tested against pomona. following challenge all 11 six-month-old non-vaccinated calves seroconverted and pomona was isolated from blood or urine on at least one occasion from nine of them. pomona was isolated once only, on the third day after challenge, from the blood of one of 11 vaccinated calves.198216030839
monoclonal antibodies suitable for incorporation into a competitive enzyme-linked immunosorbent assay (elisa) for detection of specific antibodies to leptospira interrogans serovar pomona.monoclonal antibodies (mab) were produced by fusing sp2/0-ag14 myeloma cells with spleen cells from balb/c and nd4 mice that were immunized with killed leptospira interrogans serovar pomona whole cells. thirty hybridomas which produced antibodies (of the igg1, igg2a, igg2b, or igg3 isotype) that bound to epitopes on the serovar pomona whole cell antigen were identified by an indirect enzyme-linked immunosorbent assay (elisa). twenty-eight of these 30 mabs cross-reacted in the indirect elisa with ...200010665542
three case studies involving leptospira interrogans serovar pomona infection in mixed farming units.three case studies involving leptospira interrogans serovar pomona outbreaks within mixed farming systems in south africa are described. on 2 farms, pigs constituted the main enterprise with cattle and sheep of secondary importance. on each of these 2 farms, abortion due to l. pomona in sows was confirmed by culture, and antibody titres to pomona were detected in cattle, sheep, horses and dogs. on the 3rd farm, a piggery was of secondary importance to cattle farming. abortion and death in cows o ...199910855820
competitive enzyme-linked immunosorbent assay for detection of leptospira interrogans serovar pomona antibodies in bovine sera.a competitive enzyme-linked immunosorbent assay (elisa) using a specific monoclonal antibody (m898) was developed for detection of bovine antibodies to leptospira interrogans serovar pomona. this assay was evaluated using field sera (n = 190) with serovar pomona microscopic agglutination test (mat) titers of > or =100 as the positive population (group a); field sera (n = 1,445) which were negative in the mat (1:100 dilution) for serovar pomona (group b); and sera (from a specific-pathogen-free c ...200111139193
a serological survey of leptospirosis in cattle of rural communities in the province of kwazulu-natal, south africa.a serological survey of leptospirosis in cattle originating from rural communities of the province of kwazulu-natal (kzn) in south africa was carried out between march 2001 and december 2003. the survey was designed as a 2-stage survey, using the local diptank as the primary sampling point. in total, 2021 animals from 379 diptanks in 33 magisterial districts were sampled and tested with the microscopic agglutination test (mat). the apparent prevalence at district level was adjusted for clusterin ...200919653519
diagnosis and seroprevalence of leptospirosis in california sea lions from coastal california.the sensitivity and specificity of the microscopic agglutination test (mat) as a method for detection of exposure to leptospira spp. in california sea lions (zalophus californianus) were determined. sera came from individuals that demonstrated clinical signs of renal disease, had lesions suggestive of leptospirosis at necropsy, and had visible leptospires in silver stained kidney sections as positive controls. sera from unexposed captive individuals were used as negative controls. the test was 1 ...200211838231
haemolytic disease associated with leptospira interrogans serovar pomona in red deer calves (cervus elaphus).three red deer calves (cervus elaphus) died with a haemolytic disease associated with infection by leptospira interrogans serovar pomona. infection within the herd was more prevalent than disease. sera from 16 herd mates were tested by the microscopic agglutination test (mat) and 12 had leptospiral titres, the majority to serovar pomona. a few calves had titres to balcunica and hardjo. urine was obtained for culture from six of these calves and serovar pomona was isolated from five with titres t ...198616031298
serovar distribution of a dna sequence involved in the antigenic relationship between leptospira and equine cornea.horses infected with leptospira present several clinical disorders, one of them being recurrent uveitis. a common endpoint of equine recurrent uveitis is blindness. serovar pomona has often been incriminated, although others have also been reported. an antigenic relationship between this bacterium and equine cornea has been described in previous studies. a leptospiral dna fragment that encodes cross-reacting epitopes was previously cloned and expressed in escherichia coli.200211869455
antibodies to selected viral and bacterial pathogens in european wild boars from southcentral spain.serum samples from 78 european wild boars (sus scrofa) harvested during the 1999-2000 hunting season were tested for antibodies to brucella spp., classical swine fever virus, erysipelothrix rhusiopathiae, haemophilus parasuis, leptospira interrogans serovar pomona, mycoplasma hyopneumoniae, pseudorabies virus (prv), porcine parvovirus (ppv), porcine reproductive and respiratory syndrome virus, salmonella serogroups b, c, and d, streptococcus suis, and swine influenza virus (siv) serotypes h1n1 a ...200212238391
the epidemiology of leptospira interrogans serovar pomona in grower pig epidemiological study on four farms showed that leptospiral infection became apparent in piglets from 12 weeks of age. the intensity of leptospiruria was greatest in the first 3-4 weeks of infection and from then on declined and became intermittent. factors affecting the cultural and serological prevalence of infection of the piglets were found to be the standard of hygiene and variations in the titre of the dam. dams with high titres gave better protection to their young than did those with ...199516031793
serologic evaluation of new zealand sea lions for exposure to brucella and leptospira spp.a serologic survey of anti-brucella and antileptospiral antibodies was conducted on 147 adult, female new zealand sea lions (phocarctos hookeri). most sea lions (n=138) were sampled at sandy bay, enderby island, auckland islands (50°30's, 166°17'e), january 2000-march 2005. nine were sampled at otago, new zealand (46°0's, 170°40'e); four in april 2008 and five in march 2009. serum from one of the enderby island females was weakly positive for antibodies to brucella abortus using the competitive ...201020966283
[leptospirosis as a differential diagnosis of acute renal failure].leptospirosis is a worldwide infectious disease affecting both animals and humans. leptospira pomona is well known as a pathogen of a mild form of leptospirosis (maladie des jeune porchers).200212386795
leptospirosis in northern elephant seals (mirounga angustirostris) stranded along the california coast.leptospirosis was identified in six northern elephant seals (mirounga angustirostris) that were stranded in 1995 along the coast of california (usa). histologic lesions in all seals included tubulointerstitial nephritis with tubular degeneration and necrosis. infection was confirmed through identification of spirochetes using an immunohistochemical stain for leptospira sp. antigens. one affected seal had an elevated titer to leptospira interrogans serovar pomona. four of the six seals developed ...200516107678
analysis of a leptospira interrogans locus containing dna replication genes and a new is, is1502.a region of the leptospira interrogans serovar pomona genome encoding dna replication genes was characterized. this region, designated the ppa-ntrc locus, includes 19 open reading frames and a new insertion sequence, is1502. although this locus resembles replication origins from many eubacteria, it lacks several genes common to homologous loci. some replication-related genes were previously located near rrf, and may have been moved to that location by homologous recombination between short seque ...200212399032
[outbreak of human leptospirosis after a flood in reconquista, santa fe, 1998].since april of 1998 a high number of leptospirosis cases were detected, coming from the area of reconquista central hospital in santa fe province. since january of that year a notable increase in rainfall and river levels was observed causing inundation. as screening test, macroscopic agglutination (mat) using 10 serotypes of l. interrogans. among the 122 patients studied 71 were tr positive and 52 were also elisa positive, leptospirosis diagnosis being confirmed in 40 of them. five infecting se ...200212415894
[immunogenicity and protective capacity of leptospiral whole-cell monovalent serogroup ballum vaccines in hamsters].leptospira serogroup ballum is at present the first cause of human leptospirosis in cuba. killed whole-cell vaccines were formulated with two clinical isolates of leptospira interrogans serogroup ballum using aluminum hydroxide as adjuvant. agglutinins levels induced by each vaccine in hamsters were estimated by microscopic agglutination test and specific igg activities were quantified by a whole cell-based enzyme-linked immunosorbent assay. homologous and cross protective capacity against letha ...200516502635
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