| identification of enterococcus species isolated from human infections by a conventional test scheme. | streptococci (206 cultures) previously identified as enterococci were retrieved from storage and reidentified by using tests designed to identify species of the genus enterococcus. of these 188, 91% were correctly identified as enterococcus species. of the remaining strains, nine (4%) were unidentified and six (3%) and 3 (1.5%) were identified as leuconostoc sp. and lactococcus sp., respectively. two new enterococcus species were discovered: e. raffinosus and e. solitarius. dna-dna hybridization ... | 1989 | 2656745 |
| phosphorylcholine-containing antigens in bacteria from the mouth and respiratory tract. | phosphorylcholine (pc)-containing antigens were sought in 269 bacterial isolates from the mouth and respiratory tract by an enzyme immunoassay method. only 41 (15%) isolates were pc-positive and of these 29 (70%) were strains of haemophilus influenzae. other species that produced positive results included two of five isolates of gemella haemolysans, two of five isolates of micrococcus spp., and a single strain each of bacillus sp., corynebacterium jeikeium, lactococcus sp. and h. parainfluenzae. ... | 1996 | 8544209 |
| antimicrobial activity of food-related penicillium sp. against pathogenic bacteria in laboratory media and a cheese model system. | food-related penicillium species (n = 34) and geotrichum candidum (n = 11) grown on czapek dox and brie agar were tested for their ability to suppress growth of pathogenic bacteria. ten out of 13 p. camemberti showed antagonistic activity while the other species did not interact significantly with the bacterial growth. the order of inhibition was: gram-negative bacteria and bacillus cereus > listeria monocytogenes, lactococcus sp. > micrococcus sp. whereas lactobacillus sp., staphylococcus aureu ... | 1997 | 9246777 |
| widespread distribution of the bacteriocin-producing lactic acid cocci in miso-paste products. | a survey was conducted on the ecological distribution of bacteriocin-producing lactic acid bacteria in miso-pastes, a typical fermented food in japan. methods and results nine miso-pastes were sampled for isolation of bacteriocin-producers. almost all isolated enterococcal strains produced bacteriocins but no isolated tetragenococci did so. the bacteriocin-producing isolates were divided into nine groups by phenotypic tests. as the phenotypic characters were highly diverse, these strains could n ... | 2002 | 11966910 |
| production and purification of a bacteriocin peptide produced by lactococcus sp. strain gm005, isolated from miso-paste. | lactococcus sp. gm005 was isolated from miso-paste and was found to produce a bacteriocin with strong antibacterial activity. a culture of lactococcus sp. gm005, maintained at 30 degrees c and a constant ph of 6.0, exhibited bacteriocin activity eightfold higher than that of a culture grown under ph-uncontrolled conditions. gm005 bacteriocin was purified to homogeneity on sds-page by hydrophobic column chromatography and gel filtration. the estimated molecular weight of gm005 bacteriocin was app ... | 2003 | 12927718 |
| effect of bacteriocin produced by lactococcus sp. hy 449 on skin-inflammatory bacteria. | this study was carried out to evaluate the effect of bacteriocin produced by lactococcus sp.hy 449 against skin-inflammatory bacteria such as staphylococcus epidermidis atcc 12228, staphylococcus aureus atcc 65389, streptococcus pyogenes atcc 21059, and propionibacterium acnes atcc 6919. the spot-on-the-lawn method was used to determine the antimicrobial activity of bacteriocin against indicator strains on the human skin. the bacteriocin produced by lactococcus sp. hy 449 inhibited the growth of ... | 2006 | 16226831 |
| genetic diversity among lactococcus sp. and leuconostoc sp. strains using pcr-rflp of insertion sequences iss1-type, is904, and is982. | pcr-rflp analysis of commonly occurring insertion sequences iss1-type, is904 and is982 in lactococcus sp. and leuconostoc sp. was used for the genetic differentiation of 17 strains of lactic acid bacteria. iss1-type and is982 were found in all analysed strains while is904 was present exclusively in strains belonging to lactococcus sp. amplification of iss1-type is sequences resulted in formation of about 820 bp long amplicons, except of strains lactococcus lactis ssp. lactis e and leuconostoc la ... | 2005 | 16450833 |
| effect of bacteriocin produced by lactococcus sp. hy 449 on skin-inflammatory bacteria. | this study was carried out to evaluate the effect of bacteriocin produced by lactococcus sp. hy 449 against skin-inflammatory bacteria such as staphylococcus epidermidis atcc 12228, staphylococcus aureus atcc 65389, streptococcus pyogenes atcc 21059, and propionibacterium acnes atcc 6919. the spot-on-the-lawn method was used to determine the antimicrobial activity of bacteriocin against indicator strains on the human skin. the bacteriocin produced by lactococcus sp. hy 449 inhibited the growth o ... | 2006 | 16793484 |
| the effect of decreasing alkalinity on microbial community dynamics in a sulfate-reducing bioreactor as analyzed by pcr-sscp. | pcr-single-strand conformation polymorphism (sscp) and southern blotting techniques were adopted to investigate microbial community dynamics in a sulfate-reducing bioreactor caused by decreasing influent alkalinity. experimental results indicated that the sulfate-removal rate approached 87% in 25 d under the conditions of influent alkalinity of 4000 mg/l (as caco3) and sulfate-loading rate of 4.8 g/(l*d), which indicated that the bioreactor started up successfully. the analysis of microbial comm ... | 2006 | 16989283 |
| s-adenosylmethionine directly inhibits binding of 30s ribosomal subunits to the smk box translational riboswitch rna. | the s(mk) box is a conserved riboswitch motif found in the 5' untranslated region of metk genes [encoding s-adenosylmethionine (sam) synthetase] in lactic acid bacteria, including enterococcus, streptococcus, and lactococcus sp. previous studies showed that this rna element binds sam in vitro, and sam binding causes a structural rearrangement that sequesters the shine-dalgarno (sd) sequence by pairing with an anti-sd (asd) element. a model was proposed in which sam binding inhibits metk translat ... | 2007 | 17360376 |
| identification and characterization of lactocyclicin q, a novel cyclic bacteriocin produced by lactococcus sp. strain qu 12. | lactococcus sp. strain qu 12, which was isolated from cheese, produced a novel cyclic bacteriocin termed lactocyclicin q. by using cation-exchange chromatography, hydrophobic interaction chromatography, and reverse-phase high-performance liquid chromatography, lactocyclicin q was purified from culture supernatant, and its molecular mass was determined to be 6,062.8 da by mass spectrometry. lactocyclicin q has been characterized by its unique antimicrobial spectrum, high level of protease resista ... | 2009 | 19139222 |
| investigation of mineral water springs of miercurea ciuc (csíkszereda) region (romania) with cultivation-dependent microbiological methods. | water samples of ten mineral water springs at miercurea ciuc (csíkszereda) region (romania) were examined during 2005-2006 using cultivation-dependent microbiological methods. the results of standard hygienic bacteriological tests showed that the hargita spring had perfect and five other springs had microbiologically acceptable water quality (zsögöd-, nagy-borvíz-, taploca-, szentegyháza- and lobogó springs). the water of borsáros spring was exceptionable (high germ count, presence of enterococc ... | 2010 | 20587384 |
| changes in the bacterial communities of vacuum-packaged pork during chilled storage analyzed by pcr-dgge. | in this study, pcr-denaturing gradient gel electrophoresis (dgge) was used to investigate the bacterial communities of vacuum-packaged pork during chilled storage. eight kinds of lactic acid bacteria (lab) were identified from the strains isolated from mrs plates by pcr-dgge of the v3 region, and lactobacillus sakei was the representative isolate at the end of the monitoring. by means of the direct meat analysis of pcr-dgge, lab increased gradually and carnobacterium sp./car. divergens, lactobac ... | 2010 | 20800372 |
| microbial ecology of fish species ongrowing in greek sea farms and their watery environment. | the present study focuses on the bacteriological profile of both watery ecosystem and fishes from different north and central west greek fish-farms ongrowing euryhaline fish species. the natural microflora of the fish and the water of their ongrowing units in selected farms were studied for a period of 20 months. the analyzed samples were mainly sea bream (sparus aurata) 61.3% and sea bass (dicentrarchus labrax) 24%. in most of the watery ecosystems coming from the different sampling areas, tota ... | 2011 | 21421068 |
| identification and characterization of leucocyclicin q, a novel cyclic bacteriocin produced by leuconostoc mesenteroides tk41401. | the culture supernatant of leuconostoc mesenteroides tk41401, isolated from japanese pickles, possessed antimicrobial activity against broad range of a bacterial genera and particularly strong activity against bacillus coagulans, the major contaminant of pickles. an antimicrobial peptide was purified in three chromatographic steps, and its molecular mass was determined to be 6,115.59 da by electrospray ionization time-of-flight mass spectrometry (esi-tof ms). the primary structure of this peptid ... | 2011 | 21948835 |
| spatiotemporal development of the bacterial community in a tubular longitudinal microbial fuel cell. | the spatiotemporal development of a bacterial community in an exoelectrogenic biofilm was investigated in sucrose-fed longitudinal tubular microbial fuel cell reactors, consisting of two serially connected modules. the proportional changes in the microbial community composition were assessed by polymerase chain reaction-denaturing gradient gel electrophoresis (dgge) and dna sequencing in order to relate them to the performance and stability of the bioelectrochemical system. the reproducibility o ... | 2011 | 21400098 |
| microbial community structure and population dynamics of granules developed in expanded granular sludge bed (egsb) reactors for the anaerobic treatment of low-strength wastewater at low temperature. | the anaerobic biological treatment of sucrose-based, low-strength wastewater was investigated in expanded granular sludge bed (egsb) reactors at low temperatures over a 300-day trial period. during the trial, the operating temperature was lowered in a stepwise manner from 20 degrees c to 5 degrees c. as a result, the reactors exhibited sufficient performances until 10 degrees c operation. the cod removal rate was 3.1-3.8 kgcod m(-3) day(-1) at 10 degrees c. in particular, the cod removal rate in ... | 2010 | 20390923 |