| the phylogeny of the genus clostridium: proposal of five new genera and eleven new species combinations. | the 16s rrna gene sequences of 34 named and unnamed clostridial strains were determined by pcr direct sequencing and were compared with more than 80 previously determined clostridial sequences and the previously published sequences of representative species of other low- g + c-content gram-positive genera, thereby providing an almost complete picture of the genealogical interrelationships of the clostridia. the results of our phylogenetic analysis corroborate and extend previous findings in show ... | 1994 | 7981107 |
| phylogenetic analysis of fusobacterium alocis and fusobacterium sulci based on 16s rrna gene sequences: proposal of filifactor alocis (cato, moore and moore) comb. nov. and eubacterium sulci (cato, moore and moore) comb. nov. | genes encoding the 16s rrna of fusobacterium alocis atcc 35896t and fusobacterium sulci atcc 35585t were sequenced. these sequences did not have any affinity with the 16s rrna gene sequences of members of the genus fusobacterium. fusobacterium alocis atcc 35896t and fusobacterium sulci atcc 35585t belonged to clostridium cluster xi; the species most closely related to these strains were filifactor villosus nctc 11220t and eubacterium infirmum w1471, respectively. two new combinations are propose ... | 1999 | 10555315 |
| characterisation of eubacterium-like strains isolated from oral infections. | the genus eubacterium currently includes a heterogeneous group of gram-positive, non-spore-forming anaerobic bacilli, many of which are slow growing, fastidious and generally unreactive in biochemical tests. as a consequence, cultivation and identification of isolates are difficult and the taxonomy of the group remains indifferent. in this study, 105 isolates from odontogenic infections, infections associated with dental implants or saliva from healthy subjects and provisionally assigned to the ... | 2001 | 11699590 |
| update on the taxonomy and clinical aspects of the genus fusobacterium. | the genus fusobacterium currently includes 13 species. fusobacterium nucleatum, the most frequently encountered species in humans, is heterogeneous and currently includes 5 subspecies. a potentially new subspecies of f. nucleatum that is intrinsically quinolone-resistant and phylogenetically separate from the other 5 subspecies has been identified from dog and cat oral flora. two subspecies have been described for fusobacterium necrophorum, and a new species, fusobacterium equinum, which is rela ... | 2002 | 12173104 |
| molecular analysis of bacteria in periodontitis: evaluation of clone libraries, novel phylotypes and putative pathogens. | subgingival plaque samples were obtained from 26 subjects with advanced periodontal lesions. bacterial diversity was analysed by amplification of the 16s rrna genes with two different primer sets, and subsequent cloning and sequencing. a total of 578 sequences was analysed after the exclusion of chimeras. the authors found 148 phylotypes with the clone library 27f/519r (number of clones n=322; coverage, c=66 %) and 75 phylotypes with the clone library 515f/1525r (n=256; c=84 %). comparative sequ ... | 2003 | 12576581 |
| degradation of arginine and other amino acids by butyrate-producing asaccharolytic anaerobic gram-positive rods in periodontal pockets. | the use of 20 amino acids by butyrate-producing asaccharolytic anaerobic gram-positive rods (aagprs) in periodontal pockets, i.e. eubacterium minutum, filifactor alocis, e. infirmum, e. sulci and e. saphenum, was studied. e. minutum used only arginine and lysine, and produced substantial amounts of butyrate and ammonia as the main metabolic products from arginine, and acetate, butyrate and ammonia from lysine. fi. alocis used arginine alone and produced butyrate and ammonia. e. infirmum, e. sulc ... | 2003 | 12749914 |
| detection of filifactor alocis in endodontic infections associated with different forms of periradicular diseases. | recent molecular studies have expanded the list of suspected endodontic pathogens. the aim of this study was to evaluate the occurrence of filifactor alocis in primary endodontic infections associated with different forms of periradicular diseases. identification by nested polymerase chain reaction was performed in root canal samples from teeth associated with either asymptomatic periradicular lesions or acute apical periodontitis. samples were also taken by aspiration of purulent exudate associ ... | 2003 | 12823804 |
| polymerase chain reaction-based analysis of microorganisms associated with failed endodontic treatment. | in this study, we aimed to investigate the occurrence of several microbial species in cases of failed endodontic therapy by means of the polymerase chain reaction (pcr). study design root canal samples were taken from 22 root-filled teeth with persistent periradicular lesions selected for re-treatment. dna was extracted from the samples and analyzed for the presence of 19 microbial taxa by using the polymerase chain reaction. | 2004 | 14716262 |
| selected endodontic pathogens in the apical third of infected root canals: a molecular investigation. | bacteria located at the apical portion of the root canals are conceivably in a strategic position to induce damage to the periradicular tissues and resulting inflammatory diseases. this study sought to investigate the prevalence of 11 selected putative endodontic pathogens in the apical third of infected root canals associated with periradicular lesions. the apical root portion of 23 extracted teeth with carious pulpal exposures and attached periradicular lesions was sectioned, and the root cana ... | 2004 | 15329567 |
| simultaneous detection of dialister pneumosintes and filifactor alocis in endodontic infections by 16s rdna-directed multiplex pcr. | dialister pneumosintes and filifactor alocis have been recently considered as candidate endodontic pathogens. in this study, we devised a 16s rdna-directed multiplex pcr protocol for simultaneous detection of these two bacterial species in endodontic infections. samples were taken from infected root canals associated with asymptomatic periradicular lesions as well as from cases of acute periradicular abscesses. dna extracted from the samples was used as template for simultaneous detection of d. ... | 2004 | 15564862 |
| differences in prevalence of selected bacterial species in primary endodontic infections from two distinct geographic locations. | this study intended to compare the prevalence of 7 putative endodontic pathogens in samples of primary endodontic infections taken from patients of 2 distant geographic locations. | 2005 | 15829891 |
| identification of candidate periodontal pathogens and beneficial species by quantitative 16s clonal analysis. | most studies of the bacterial etiology of periodontitis have used either culture-based or targeted dna approaches, and so it is likely that pathogens remain undiscovered. the purpose of this study was to use culture-independent, quantitative analysis of biofilms associated with chronic periodontitis and periodontal health to identify pathogens and beneficial species. samples from subjects with periodontitis and controls were analyzed using ribosomal 16s cloning and sequencing. several genera, ma ... | 2005 | 16081935 |
| a new checkerboard panel for testing bacterial markers in periodontal disease. | various microbiological methods have been used for testing bacterial markers for periodontitis and periodontal disease progression. most studies have used only a limited number of well recognized bacterial species. the purpose of the present study was to evaluate the association of 13 more recently identified bacterial species in a new panel in comparison with 12 previously more recognized periodontotopathogens ('old panel') using the 'checkerboard' dna-dna hybridization method. | 2006 | 16390335 |
| molecular analysis of bacteria in asymptomatic and symptomatic endodontic infections. | the purpose of the present study was to use terminal restriction fragment length polymorphism analysis and the 16s rrna gene clone library to investigate the diversity of the microbiota associated with asymptomatic and symptomatic endodontic infections and to compare the bacterial community structure in these two clinical conditions. samples were taken from asymptomatic endodontic infections associated with chronic periradicular lesions and from symptomatic infections clinically diagnosed as acu ... | 2006 | 16476021 |
| molecular analysis of filifactor alocis, tannerella forsythia, and treponema denticola associated with primary endodontic infections and failed endodontic treatment. | the aim of this study was to investigate the presence of strict anaerobes such as filifactor alocis, tannerella forsythia, and treponema denticola in primary and secondary root-infected canals with periapical lesions by molecular analysis and the association of these species with specific endodontic signs and symptoms. microbial samples were taken from 100 root canals, 50 with necrotic pulp tissues (npt, primary infection), and 50 with failed endodontic treatment (fet, secondary infection). dna ... | 2006 | 16982268 |
| changes in periodontal health status are associated with bacterial community shifts as assessed by quantitative 16s cloning and sequencing. | the gingival sulcus contains a complex ecosystem that includes many uncultivated bacteria. understanding the dynamics of this ecosystem in transitions between health and disease is important in advancing our understanding of the bacterial etiology of periodontitis. the objective of this longitudinal study was to examine the stability of bacterial colonization in the gingival crevice and to explore the relationship between shifts in microbial composition and changes in periodontal health status u ... | 2006 | 17021095 |
| prevalence of selected bacterial named species and uncultivated phylotypes in endodontic abscesses from two geographic locations. | evidence is mounting that the prevalence of some putative endodontic pathogens can significantly vary according to the geographic location in which samples were taken. this study aimed to provide additional knowledge on this subject by comparing the frequencies of 10 selected candidate endodontic pathogens in samples of acute apical abscesses obtained from two distinct geographic locations; portland, or, and rio de janeiro, brazil. dna was extracted from aspirates and used as template in nested ... | 2006 | 17174667 |
| bacterial examination of endodontic infections by clonal analysis in concert with denaturing high-performance liquid chromatography. | the aim of this study was to examine the diversity of bacterial species in the infected root canals of teeth associated with endodontic abscesses by cloning and sequencing techniques in concert with denaturing high-performance liquid chromatography. | 2007 | 17949344 |
| microbial analysis of canals of root-filled teeth with periapical lesions using polymerase chain reaction. | the objective of the present study was to investigate the presence of nine bacterial species in root-filled teeth associated with periapical lesions using a polymerase chain reaction analysis and to correlate these species with clinical features of the cases. dna was extracted from 45 canal samples of root-filled teeth with periapical lesions. a pcr assay using species-specific primers of 16s rdna and the downstream intergenic spacer region was used for microbial detection. enterococcus faecalis ... | 2008 | 18436030 |
| bacteria in the apical root canal of teeth with primary apical periodontitis. | bacteria settled in the apical root canal are in a privileged position to inflict damage to the periradicular tissues. therefore, the species identified in this region can be of special relevance for the pathogenesis of apical periodontitis. this study investigated the occurrence and levels of several bacterial taxa in the apical root canal of teeth with apical periodontitis. | 2009 | 19426923 |
| comparisons of subgingival microbial profiles of refractory periodontitis, severe periodontitis, and periodontal health using the human oral microbe identification microarray. | this study compared the subgingival microbiota of subjects with refractory periodontitis (rp) to those in subjects with treatable periodontitis (grs = good responders) or periodontal health (ph) using the human oral microbe identification microarray (homim). | 2009 | 19722792 |
| filifactor alocis--involvement in periodontal biofilms. | bacteria in periodontal pockets develop complex sessile communities that attach to the tooth surface. these highly dynamic microfloral environments challenge both clinicians and researchers alike. the exploration of structural organisation and bacterial interactions within these biofilms is critically important for a thorough understanding of periodontal disease. in recent years, filifactor alocis, a fastidious, gram-positive, obligately anaerobic rod was repeatedly identified in periodontal les ... | 2010 | 20193074 |
| a microbiological study in relation to the presence of caries and calculus. | to examine whether oral microflora in individuals with caries differs from that of individuals with calculus in thai adolescents with poor oral hygiene. | 2010 | 20384532 |
| response of subgingival bacteria to smoking cessation. | it has been demonstrated that smoking cessation alters the subgingival microbial profile; however, the response of individual bacteria within this ecosystem has not been well studied. the aim of this investigation, therefore, was to longitudinally examine the effect of smoking cessation on the prevalence and levels of selected subgingival bacteria using molecular approaches for bacterial identification and enumeration. subgingival plaque was collected from 22 smokers at the baseline and 12 month ... | 2010 | 20410352 |
| antigenic activity of bacterial endodontic contents from primary root canal infection with periapical lesions against macrophage in the release of interleukin-1beta and tumor necrosis factor alpha. | periradicular tissue chronic stimulation by endotoxin may cause apical periodontitis. this study investigated the microbial profile and the levels of endotoxin found in primary root canal infection with apical periodontitis, determined their antigenicity against macrophages through the levels of interleukin (il)-1beta and tumor necrosis factor alpha (tnf-alpha), and evaluated their relationship with clinical and radiographic findings. | 2010 | 20728711 |
| filifactor alocis has virulence attributes that can enhance its persistence under oxidative stress conditions and mediate invasion of epithelial cells by porphyromonas gingivalis. | filifactor alocis, a gram-positive anaerobic rod, is one of the most abundant bacteria identified in the periodontal pockets of periodontitis patients. there is a gap in our understanding of its pathogenicity and ability to interact with other periodontal pathogens. to evaluate the virulence potential of f. alocis and its ability to interact with porphyromonas gingivalis w83, several clinical isolates of f. alocis were characterized. f. alocis showed nongingipain protease and sialidase activitie ... | 2011 | 21825062 |
| distinct and complex bacterial profiles in human periodontitis and health revealed by 16s pyrosequencing. | periodontitis has a polymicrobial etiology within the framework of a complex microbial ecosystem. with advances in sequencing technologies, comprehensive studies to elucidate bacterial community differences have recently become possible. we used 454 sequencing of 16s rrna genes to compare subgingival bacterial communities from 29 periodontally healthy controls and 29 subjects with chronic periodontitis. amplicons from both the v1-2 and v4 regions of the 16s gene were sequenced, yielding 1 393 57 ... | 2011 | 22170420 |
| Filifactor alocis interactions with gingival epithelial cells. | An association between the gram-positive anaerobe Filifactor alocis and periodontal disease has recently emerged; however, possible pathogenic mechanisms have not been investigated. In this study we examined the responses of primary cultures of gingival epithelial cells (GECs) to infection with F. alocis. Secretion of the pro-inflammatory cytokines interleukin-1ß, interleukin-6 and tumor necrosis factor-a from GECs was stimulated by F. alocis infection. F. alocis also induced apoptosis in GECs t ... | 2011 | 22053964 |