| purification and some properties of bacillus macerans cycloamylose (cyclodextrin) glucanotransferase. | bacillus macerans cycloamylose (cyclodextrin) glucanotransferase (ec 2.4.1.19) was purified by the technique of starch adsorption and deae-cellulose column chromatography, and then crystallized from an ammonium sulfate solution containing mm calcium chloride. the crystals of the enzyme were rod-shaped and showed a single band by disc-gel electrophorsis. the purified enzyme was dissociated into two subunits by sodium dodecyl sulfate-disc electrophoresis. the subunits had no enzyme activity. detai ... | 1978 | 25712 |
| purification and physiocochemical properties of an extra-cellular cycloamylose (cyclodextrin) glucanotransferase from bacillus macerans. | an extracellular cycloamylose (cyclodextrin) glucanotransferase (ec 2.4.1.19) from bacillus macerans was purified to homogeneity by adsorption on starch, ammonium sulfate fractionation, column chromatography on deae-cellulose, and gel filtration on sephadex g-100. the enzyme had a molecular weight of 67,000 and consisted of one polypeptide chain. the isoelectric point was ph 5.4. temperature and ph optima were 60 degrees and 5.4--5.8, respectively. the purified enzyme was quite stable at 50 degr ... | 1979 | 39676 |
| fine structure of mesosomal involvement during bacillus macerans sporulation. | the ultrastructure of endospore formation in bacillus macerans atcc 8244 is characterized by the examination of thin sections of cells grown synchronously in a defined medium. for the most part, sporulation in this organism proceeds as described in other bacillus species. however, unusually extensive mesosomal involvement occurs during the early stages of sporulation, through the completion of engulfment. a large mesosome is associated with spore septum formation and a portion of this mesosome i ... | 1975 | 234941 |
| [cyclodextrin glucanotransferase from klebsiella pneumoniae. 1. formation, purification and properties of the enzyme from klebsiella pneumoniae m 5 al (author's transl)]. | 1. the strain m 5 al of klebsiella pneumoniae grows excellently with starches. we were able to show that besides the pullulanase associated with the external membrane of the cells the bacterium produces an inducible, extracellular cyclodextrin glucanotransferase [1,4-alpha-d-glucan-4-alpha-(1,4-alpha-glucano)-transferase (cyclising) (ec 2.4.1.19)]. potato starch and cyclohexaamylose or cycloheptaamylose were found to be the best "inducing" carbon sources for the synthesis of the enzyme. when the ... | 1977 | 319771 |
| catabolism of protocatechuate by bacillus macerans. | an aerobic endospore-forming bacterium, tentatively identified as a strain (jj-lb) of bacillus macerans, was isolated by enrichment on 4-hydroxybenzoate (4hba), using as an inoculum soil taken from a 50 degrees c iadho hot spring. enzymatic analyses of cells grown on succinate and 4hba indicated that strain jj-1b degrades 4hba by way of the novel protocatechuate (pca) 2,3-dioxygenase pathway. purification of the pca 2,3-dioxygenase by affinity chromatography allowed the first observation of the ... | 1979 | 453834 |
| intracolonic tensions of oxygen and carbon dioxide in germfree, conventional, and gnotobiotic rats. | the relation of intracolonic gaseous tension to fecal microflora was investigated by mass spectrometric measurements of intracolonic o2 and co2 in unanesthetized germfree, conventional, and gnotobiotic rats; ip measurements were obtained in rats whose colons became perforated accidentally; fecal bacterial flora and cecal size were also determined. gnotobiotes were monoassociated with escherichia coli, bacteroides fragilis and staphylococcus epidermidis, and were diassociated with e. coli plus b. ... | 1976 | 766018 |
| nitrogen fixation associated with grasses in oregon. | nitrogen fixation associated with both natural grasslands and grain crops of oregon was studied using the acetylene-reduction assay. a number of the grasses collected has some acetylene-reducing activity. agrostis tenuis sibth. had substantially greater activity than any of the other species, with a mean rate estimated at 37 g n2 fixed per hectare per day. assuming 100 days of activity, about 3 kg of n2 would be fixed per hectare per year. this quantity of nitrogen may be important in the mainte ... | 1976 | 1260544 |
| acceptor specificity of cyclodextrin glycosyltransferase from bacillus stearothermophilus and synthesis of alpha-d-glucosyl o-beta-d-galactosyl-(1----4)-beta-d-glucoside. | bacillus stearothermophilus cgtase had a wider acceptor specificity than bacillus macerans cgtase did and produced large amounts of transfer products of various acceptors such as d-galactose, d-mannose, d-fructose, d- and l-arabinose, d- and l-fucose, l-rhamnose, d-glucosamine, and lactose, which were inefficient acceptors for b. macerans cgtase. the main component of the smallest transfer products of lactose was assumed to be alpha-d-glucosyl o-beta-d-galactosyl-(1----4)-beta-d-glucoside. | 1992 | 1368942 |
| a novel and efficient method for enzymatic synthesis of high purity maltose using moranoline (1-deoxynojirimycin). | a transglycosylation reaction with moranoline (1-deoxynojirimycin) was done with soluble starch as the glucosyl donor and bacillus macerans amylase as a cyclodextrin glycosyltransferase [ec 2.4.1.19]. the resultant transglycosylation products with moranoline, obtained by treating the reaction mixture with a strong cation exchange resin, were hydrolyzed by beta-amylase [ec 3.2.1.2] from sweet potatoes. the hydrolysate was treated with a strong cation exchange resin, and high purity maltose was ob ... | 1992 | 1368945 |
| enzymatic synthesis of 2-chloro-4-nitrophenyl 4,6-o-3-ketobutylidene beta-maltopentaoside, a substrate for alpha-amylase. | a transglycosylation reaction with 2-chloro-4-nitrophenyl beta-maltoside as an acceptor was done with 4,6-o-3-ketobutylidene maltopentaose and bacillus macerans cyclodextrin glucanotransferase in an aqueous solution containing 50% n-propanol, and there were two main transglycosylation products. they were identified as 2-chloro-4-nitrophenyl 4,6-o-3-ketobutylidene beta-maltopentaoside and 2-chloro-4-nitrophenyl 4,6-o-3-ketobutylidene beta-maltohexaoside, and their yields were 30% and 21% respecti ... | 1992 | 1369056 |
| rapid in situ hybridization technique using 16s rrna segments for detecting and differentiating the closely related gram-positive organisms bacillus polymyxa and bacillus macerans. | a rapid, sensitive, inexpensive in situ hybridization technique, using 30-mer 16s rrna probes, can specifically differentiate two closely related bacillus spp., b. polymyxa and b. macerans. the 16s rrna probes were labeled with a rhodamine derivative (texas red), and quantitative fluorescence measurements were made on individual bacterial cells. the microscopic fields analyzed were selected by phase-contrast microscopy, and the fluorescence imaging analyses were performed on 16 to 67 individual ... | 1992 | 1381173 |
| enzymatic synthesis of low molecular weight amyloses with modified terminal groups. | low molecular weight amyloses with modified terminal groups were synthesized by cyclomaltohexaose (alpha-cyclodextrin) transfer using (1----4)-alpha-d-glucan: 4-alpha-d-(1----4)- alpha-d-glucopyranosyltransferase (cyclising) (ec 2.4.1.19) from bacillus macerans. 4-nitrophenyl alpha-malto-oligosaccharides d.p. 2-7 served as acceptors, and cyclomaltohexaose served as the donor. the reaction was optimized to obtain a majority of species of definite chain lengths in a range of d.p. 10-20, depending ... | 1992 | 1386787 |
| biochemical activities of bacillus species isolated from flat sour evaporated milk. | forty strains of bacilli were isolated from flat sour evaporated milk and were characterized as bacillus stearothermophilus (5 strains), bacillus licheniformis (10 strains), bacillus coagulans (15 strains), bacillus macerans (5 strains), and bacillus subtilis (5 strains). the hydrolases of these strains were examined with the api zym system, and the ability of these strains to produce acid in milk incubated at different temperatures was also examined. esterase, esterase lipase, lipase, valine am ... | 1992 | 1430475 |
| cyclization characteristics of cyclodextrin glucanotransferase are conferred by the nh2-terminal region of the enzyme. | cyclodextrin glucanotransferase (cgtase; ec 2.4.1.19) is produced mainly by bacillus strains. cgtase from bacillus macerans ifo3490 produces alpha-cyclodextrin as the major hydrolysis product from starch, whereas thermostable cgtase from bacillus stearothermophilus no2 produces alpha- and beta-cyclodextrins. to analyze the cyclization characteristics of cgtase, we cloned different types of cgtase genes and constructed chimeric genes. cgtase genes from these two strains were cloned in bacillus su ... | 1992 | 1476442 |
| the metabolism of anthracene and 9,10-dimethylanthracene by bacteria isolated from waters. | the metabolism of two polycyclic aromatic hydrocarbons i.e. anthracene and 9,10-dimethylanthracene by micrococcus sp., pseudomonas sp. and bacillus macerans was examined. the above compounds were used as a sole carbon source for their growth. using the reversed-phase thin layer chromatography techniques a number of anthracene and 9,10-dimethylanthracene metabolites were isolated and their structures identified spectroscopically. these included anthracene and 9,10-dimethylanthracene cis-dihydrodi ... | 1991 | 1726622 |
| [screening of bacillus proteases with plasmin and activator types of action on fibrin]. | forty cultures of aerobic sporogenic bacteria were screened for the plasmin and activator action of bacillar proteases on fibrin. the microorganisms were grown in chemically defined and complex potato-peptone media. some active cultures were found to produce enzymes with both plasmin and activator types of action. the following organisms exerted the highest activity of the plasmin type; bacillus macerans pi, strain p3, b. licheniformis 125 and b. firmus. the most active enzymes with the activato ... | 1991 | 1832733 |
| investigation of the active site of bacillus macerans cyclodextrin glucanotransferase by use of modified maltooligosaccharides. | the active site of bacillus macerans cyclodextrin glucanotransferase (cgtase) was examined by use of derivatives of phenyl alpha-maltopentaoside and phenyl alpha-glucoside as the substrates and acceptors, respectively. the active site of this enzyme is considered to be composed of tandem subsites (s4, s3, s2, s1, s1', s2', etc.) geometrically complementary to several glucose residues, and the alpha-1,4-glycosidic linkage of a substrate is split between s1 and s1'. the features of subsites s3 and ... | 1991 | 1838375 |
| [monoclonal antibodies to cyclodextrin-glycosyltransferase from bacillus macerans]. | cyclodextrin glycosyltransferase (cgtase, ec 2.4.1.19) was isolated from the cell-free culture medium of bacillus macerans strain bkmb-506. the purified enzyme was used to immunize balb/c mice. polyclonal antibodies (pabs) and 15 hybridoma cells producing monoclonal antibodies (mabs) against cgtase were obtained. the properties of mabs were studied using elisa and immunoblotting. a method for detecting small amounts of cgtase was developed. the interactions of pabs and mabs with cgtase of bac. c ... | 1991 | 1839608 |
| structure of the beta-1,3-1,4-glucanase gene of bacillus macerans: homologies to other beta-glucanases. | the nucleotide sequence of an 852 base pair (bp) dna fragment containing the entire gene coding for thermostable beta-1,3-1,4-glucanase of bacillus macerans has been determined. the bglm gene comprises an open reading frame (orf) of 711 bp (237 codons) starting with atg at position 93 and extending to the translational stop codon taa at position 804. the deduced amino acid sequence of the mature protein shows 70% homology to published sequences of mesophilic beta-1,3-1,4-glucanases from b. subti ... | 1990 | 2274030 |
| protocatechuate 2,3-dioxygenase from bacillus macerans. | | 1990 | 2280722 |
| efficiency factors and atp/adp ratios in nitrogen-fixing bacillus polymyxa and bacillus azotofixans. | the efficiency factor, the number of moles of atp generated per mole of glucose fermented, was determined in anaerobic, non-carbon-limited n2-fixing cultures of bacillus polymyxa, bacillus macerans, bacillus azotofixans, and clostridium butyricum through identification and quantitation of the fermentation products by 13c nuclear magnetic resonance spectroscopy and measurement of acetate kinase activities. all three bacillus species had acetate kinase activities and produced acetate and ethanol a ... | 1990 | 2318806 |
| bacillus thiaminolyticus sp. nov., nom. rev. | the name "bacillus thiaminolyticus" kuno 1951 was not included on the approved lists of bacterial names and has lost standing in bacteriological nomenclature. the genetic homogeneity of "bacillus thiaminolyticus" was assessed by determining guanine-plus-cytosine contents by the buoyant density method and by measuring dna relatedness by using spectrophotometric reassociation procedures. of the 26 strains which i studied, 24 had guanine-plus-cytosine contents in the range from 52 to 54 mol%. the c ... | 1990 | 2397192 |
| ammonia assimilation pathways in nitrogen-fixing clostridium kluyverii and clostridium butyricum. | pathways of ammonia assimilation into glutamic acid were investigated in ammonia-grown and n2-fixing clostridium kluyverii and clostridium butyricum by measuring the specific activities of glutamate dehydrogenase, glutamine synthetase, and glutamate synthase. c. kluyverii had nadph-glutamate dehydrogenase with a km of 12.0 mm for nh4+. the glutamate dehydrogenase pathway played an important role in ammonia assimilation in ammonia-grown cells but was found to play a minor role relative to that of ... | 1989 | 2564848 |
| role of glutamate dehydrogenase in ammonia assimilation in nitrogen-fixing bacillus macerans. | pathways of ammonia assimilation into glutamic acid in bacillus macerans were investigated by measurements of the specific activities of glutamate dehydrogenase (gdh), glutamine synthetase, and glutamate synthase. in ammonia-rich medium, gdh was the predominant pathway of ammonia assimilation. in nitrogen-fixing cells in which the intracellular nh4+ concentration was 1.4 +/- 0.5 mm, the activity of gdh with a km of 2.2 mm for nh4+ was found to be severalfold higher than that of glutamate synthas ... | 1987 | 2888750 |
| glutamate biosynthesis in bacillus azotofixans. 15n nmr and enzymatic studies. | pathways of ammonia assimilation into glutamic acid in bacillus azotofixans, a recently characterized nitrogen-fixing species of bacillus, were investigated through observation by nmr spectroscopy of in vivo incorporation of 15n into glutamine and glutamic acid in the absence and presence of inhibitors of ammonia-assimilating enzymes, in combination with measurements of the specific activities of glutamate dehydrogenase, glutamine synthetase, glutamate synthase, and alanine dehydrogenase. in amm ... | 1988 | 2893794 |
| the branched malto-oligosaccharides resulting from the action of bacillus macerans cycloamylose glucanotransferase on 6-o-alpha-d-glucopyranosyl-cyclomaltohexaose plus d-glucose. | | 1988 | 2965971 |
| molecular cloning and nucleotide sequence of the cyclomaltodextrin glucanotransferase gene from the alkalophilic bacillus sp. strain no. 38-2. | the cyclomaltodextrin glucanotransferase (cgtase, ec 2.4.1.19) gene from the alkalophilic bacillus sp. strain no. 38-2 was cloned in escherichia coli using pbr322. a plasmid, pcs8, was isolated from a transformant producing cgtase and the cloned cgtase gene was found to be in a 5.3 kb dna fragment. the nucleotide sequence of a 2.5 kb segment encoding the cgtase was determined. this segment showed an open reading frame which would encode a polypeptide of 712 amino acids. the pcs8 cgtase had the s ... | 1988 | 2972812 |
| molecular cloning, dna nucleotide sequencing, and expression in bacillus subtilis cells of the bacillus macerans cyclodextrin glucanotransferase gene. | the gene for cyclodextrin glucanotransferase from bacillus macerans was cloned in an escherichia coli bacteriophage, lambda d69, and was recloned in a bacillus subtilis plasmid, pub110. starting from an atg initiation codon, a unique reading frame was shown to extend for 2,142 base pairs (714 amino acids). the nucleotide sequence revealed that the enzyme is composed of two identical subunits. | 1986 | 3011735 |
| high-resolution solid-state 13c nuclear magnetic resonance of bacterial spores: identification of the alpha-carbon signal of dipicolinic acid. | natural-abundance solid-state 13c nuclear magnetic resonance spectra were obtained for bacterial spores for the first time by using the technique of cross-polarization magic-angle-spinning nuclear magnetic resonance spectroscopy. a resonance at about 150 ppm, detectable in spore samples having a mn content of less than 0.05%, was consistent with an identification as the alpha-carbon signal of calcium dipicolinate; this signal was missing from a spore sample treated with acid to release dipicolin ... | 1988 | 3132103 |
| preparation of non-reducing-end substituted p-nitrophenyl alpha-maltopentaoside (fg5p) as a substrate for a coupled enzymatic assay for alpha-amylases. | p-nitrophenyl o-6-deoxy-6-[(2-pyridyl)amino]-alpha-d-glucopyranosyl-(1----4)-o-alpha-d - glucopyranosyl-(1----4)-o-alpha-d-glucopyranosyl-(1----4)-o-alpha-d- glucopyranosyl-(1----4)-alpha-d-glucopyranoside, fg5p, was prepared, taking advantage of the action of bacillus macerans cyclodextrin glucanotransferase on a mixture of o-6-deoxy-6-[(2-pyridyl)-amino]-alpha-d-glucopyranosyl-(1----4)-o-alpha- d- glucopyranosyl-(1----4)-o-alpha-d-glucopyranosyl-(1----4)-o-alpha-d- glucopyranosyl-(1----4)-o-al ... | 1985 | 3161874 |
| germination requirements of bacillus macerans spores. | 2-phenylacetamide is an effective germinant for spores of five strains of bacillus macerans, particularly in the presence of fructose. benzyl penicillin, the phenyl acetamide derivative of penicillin, and phenylacetic acid are also good germinants. l-asparagine is an excellent germinant for four strains. alpha-amino-butyric acid is moderately effective. pyridoxine, pyridoxal, adenine, and 2,6-diaminopurine are potent germinants for nca strain 7x1 only. d-glucose is a powerful germinant for strai ... | 1971 | 4251279 |
| modified two-phase system for partition of bacillus macerans spores. | an aqueous two-phase system made with polyethylene glycol (peg) 1000 and potassium phosphate gives much higher recovery of bacillus macerans spores in the upper phase (peg rich) than does a similar system utilizing peg 4000. the upper phase completely rejects vegetative cells, which collect at the interface. the system may be useful in purifying spores of other species. scanning electron micrographs of b. macerans spores cleaned in this system show no obvious attached sporangial fragments. the m ... | 1969 | 4907003 |
| purification and properties of the cyclodextrinase of bacillus macerans. | | 1968 | 4922856 |
| unusual growth and metabolism of the biotin-deficient bacillus macerans. | | 1969 | 5350537 |
| pattern of action of the amylase and the cyclodextrinase of bacillus macerans. | | 1968 | 5649518 |
| purification and properties of the amylase of bacillus macerans. | | 1968 | 5758537 |
| adenine and 2,6-diaminopurine as germinants for bacillus macerans spores. | | 1967 | 6066056 |
| cyclodextrin glycosyltransferases from klebsiella pneumoniae m 5 al and bacillus macerans: quantitative analysis by high-performance liquid chromatography of the (1 leads to 4)-alpha-d-glucopyranosyl transfer-products from some linear and cyclic substrates. | the analysis of the (1 leads to 4)-alpha-d-glucopyranosyl transfer-products from some linear and cyclic substrates by quantitative h.p.l.c. illuminated the mode of action of the cyclodextrin glycosyltransferases [1 leads to 4)-alpha-d-glucan:[(1 leads to 4)-alpha-d-glucopyranosyl]transferase (cyclising), ec 2.4.1.19) from klebsiella pneumoniae m 5 al and bacillus macerans. d-glucopyranosyl transfer, obligatory for maltose (poor substrate), was preferred for maltotriose (good substrate). the leng ... | 1983 | 6224557 |
| microculture model studies on the effect of various gas atmospheres on microbial growth at different temperatures. | a microculture technique, employing 96-well tissue culture plates in plastic bags, was used to test the effect of different gas atmospheres (vacuum, air, nitrogen, and carbon dioxide) on the growth of escherichia coli, bacillus macerans, salmonella typhimurium. candida albicans, lactobacillus plantarum, pseudomonas/acinetobacter/moraxella-group, brochothrix thermosphacta and yersinia enterocolitica at 2, 6, and 20 degrees c. in general, carbon dioxide was the most effective inhibitor. the inhibi ... | 1983 | 6413475 |
| glycan modification of a thermostable recombinant (1-3, 1-4)-beta-glucanase secreted from saccharomyces cerevisiae is determined by strain and culture conditions. | high level biosynthesis and secretion of the thermostable hybrid (1-3, 1-4)- beta-glucanase h(a16-m) has been achieved in saccharomyces cerevisiae by means of the yeast vacuolar endoprotease b promoter (prb1p) and the bacillus macerans (1-3, 1-4)-beta-glucanase signal peptide. the n-glycans present on the yeast-secreted h(a16-m), denoted h(a16-m)-y, were released by endoglycosidase h, and identified by proton nmr spectroscopy to be a homologous series of man8-13glcnac2, although only traces of m ... | 1995 | 7496153 |
| cyclodextrin glycosyltransferase may be the only starch-degrading enzyme in bacillus macerans. | cyclodextrin glycosyltransferase (cgtase) was released into the culture fluid by bacillus macerans predominantly in the late stationary phase of growth and during autolysis in the presence of either glucose or starch as a carbon source. in both cases significant soluble intracellular enzyme activity could be observed in the early stationary phase, and a low non-soluble intracellular cgtase activity could be demonstrated also in the exponential growth phase in the presence of starch. at the end o ... | 1995 | 7536419 |
| crystal structure of bacillus licheniformis 1,3-1,4-beta-d-glucan 4-glucanohydrolase at 1.8 a resolution. | the crystal structure of the 1,3-1,4-beta-d-glucan 4-glucanohydrolase from bacillus licheniformis is solved at a resolution of 1.8 a and refined to r = 16.5%. the protein has a similar beta-sandwich structure as the homologous enzyme from bacillus macerans and the hybrid h(a16-m). this demonstrates that the jellyroll fold of these proteins is remarkably rigid and only weakly influenced by crystal contacts. the crystal structure permits to extend mechanistic considerations derived for the b. lich ... | 1995 | 7589539 |
| brain abscess due to bacillus macerans following a penetrating periorbital injury. | we report a case of a brain abscess due to bacillus macerans and clostridium sp. following a penetrating periorbital injury by a wooden branch. intracranial penetration by and retention of a foreign body were not suspected initially, and neurological symptoms developed only 2.5 months later. previously reported cases of brain abscesses due to bacillus species are reviewed. | 1995 | 7665681 |
| influence of ca2+ on conformation and stability of three bacterial hybrid glucanases. | the three hybrid glucanases (1-12)amy x mac(13-214), (1-12)amy x des-tyr13mac(14-214); (1-16)amy x mac(17-214) are composed of short n-terminal segments of 12 or 16 amino acid residues derived from the bacillus amyloliquefaciens glucanase (amy) and of residues 13-214, 14-214 and 17-214, respectively, derived from the bacillus macerans enzyme (mac). the three proteins have similar conformational features as shown by the similar characteristics of their cd spectra in the far- and near-ultraviolet ... | 1995 | 7758469 |
| production and immobilization of a proteinase-reduced cyclodextrin glycosyltransferase preparation. | cyclodextrin glycosyltransferase (cgtase) was produced by a 3-day cultivation of bacillus macerans growing in a natural medium containing grated-potatoes. besides cgtase the culture supernatant contained a mixture of serine proteinases with a predominant subtilisin-like activity. by fractionated precipitation with ammonium sulphate the cgtase (molecular mass approximately 70 kda) was concentrated and largely separated from the proteinases (molecular mass approximately 28 kda). among the various ... | 1993 | 7763552 |
| crystal structure and site-directed mutagenesis of bacillus macerans endo-1,3-1,4-beta-glucanase. | in beta-glucans those beta-1,4 glycosidic bonds which are adjacent to beta-1,3 bonds are cleaved by endo-1,3-1,4-beta-glucanases (beta-glucanases). here, the relationship between structure and activity of the beta-glucanase of bacillus macerans is studied by x-ray crystallography and site-directed mutagenesis of active site residues. crystal structure analysis at 2.3-a resolution reveals a jelly-roll protein structure with a deep active site channel harboring the amino acid residues trp101, glu1 ... | 1995 | 7852389 |
| native-like in vivo folding of a circularly permuted jellyroll protein shown by crystal structure analysis. | a jellyroll beta-sandwich protein, the bacillus beta-glucanase h(a16-m), is used to probe the role of n-terminal peptide regions in protein folding in vivo. a gene encoding h(a16-m) is rearranged to place residues 1-58 of the protein behind a signal peptide and residues 59-214. the rearranged gene is expressed in escherichia coli. the resultant circularly permuted protein, cpa16m-59, is secreted into the periplasm, correctly processed, and folded into a stable and active enzyme. crystal structur ... | 1994 | 7937966 |
| microcalorimetric determination of the thermostability of three hybrid (1-3,1-4)-beta-glucanases. | thermodynamic parameters of the three hybrid (1-3,1-4)-beta-glucanases h(a12-m), h(a12-m) delta y13, and h(a16-m) composed of short n-terminal regions derived from the bacillus amyloliquefaciens enzyme and a c-terminal region of the homologous bacillus macerans enzyme were determined in 2 mm sodium cacodylate ph 6.0, 1.5m guanidine hydrochloride, containing 1 mm cacl2 or 1 mm edta. melting of h(a12-m) delta y13 and h(a16-m) in the presence of calcium ions is characterized by two subtransitions; ... | 1994 | 7946082 |
| identification of active site carboxylic residues in bacillus licheniformis 1,3-1,4-beta-d-glucan 4-glucanohydrolase by site-directed mutagenesis. | active site residues of 1,3-1,4-beta-d-glucan 4-glucanohydrolase (ec 3.2.1.73) from bacillus licheniformis have been identified by site-directed mutagenesis. previous work revealed that glu-134 was essential for enzymatic activity, and it was proposed as the catalytic nucleophile by affinity labeling of the highly homologous bacillus amyloliquefaciens enzyme. to search for the general acid catalyst, the asp and glu residues conserved among the bacillus isozymes have been mutated to asn and gln, ... | 1994 | 8182059 |
| purification and characterization of protocatechuate 2,3-dioxygenase from bacillus macerans: a new extradiol catecholic dioxygenase. | protocatechuate 2,3-dioxygenase (2,3-pcd) from bacillus macerans jj1b has been purified to homogeneity for the first time. the enzyme catalyzes proximal extradiol ring cleavage of protocatechuate (pca) with the attendant incorporation of both atoms of oxygen from o2. the holoenzyme has a mass of 143 +/- 7 kda as determined by ultracentrifugation and other techniques. it is composed of four apparently identical subunits with m(r)s of 35,500, each containing one iron atom. mössbauer spectroscopy o ... | 1993 | 8392511 |
| a polyphasic reassessment of the genus paenibacillus, reclassification of bacillus lautus (nakamura 1984) as paenibacillus lautus comb. nov. and of bacillus peoriae (montefusco et al. 1993) as paenibacillus peoriae comb. nov., and emended descriptions of p. lautus and of p. peoriae. | seventy-seven strains representing 10 species in the paenibacillus polymyxa 16s rrna group and 3 other species that exhibit phenetic relatedness to members of this group, bacillus lautus, "bacillus longisporus," and bacillus peoriae, were characterized genotypically and phenotypically by performing an amplified ribosomal dna restriction analysis, a randomly amplified polymorphic dna analysis, a fatty acid methyl ester analysis, sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole-c ... | 1996 | 8863428 |
| pharmacological and biochemical characterization of the mouse 5ht5a serotonin receptor heterologously produced in the yeast saccharomyces cerevisiae. | the cdna for the mouse 5ht5a receptor has been functionally expressed in the unicellular yeast saccharomyces cerevisiae. the nh2-terminal end of the receptor gene was fused to the bacillus macerans (1-3, 1-4)-beta-glucanase signal sequence to ensure proper membrane insertion and to the c-myc epitope to permit immunological detection of the heterologously expressed protein. in the resulting episomal yeast expression plasmid pcnnmm5ht5a the modified 5ht5a gene is under the transcriptional control ... | 1996 | 8865364 |
| bacterial contaminants in liquid packaging boards: assessment of potential for food spoilage. | liquid packaging boards and blanks were examined for microbial contaminants. a total of 218 strains were identified and representatives of the most frequent species were characterized for their potential for food spoilage. contaminants found were aerobic spore-forming bacteria, mostly bacillus megaterium, b. licheniformis, b. cereus group, b. pumilus, paenibacillus macerans, p. polymyxa, p. pabuli and b. flexus. production of amylolytic, proteolytic, lipolytic and phospholipolytic enzymes was co ... | 1996 | 8896355 |
| individual amino acids in the n-terminal loop region determine the thermostability and unfolding characteristics of bacterial glucanases. | thermostability and unfolding behavior of the wild-type (1,3-1,4)-beta-glucanases from bacillus macerans (mac) and bacillus amyloliquefaciens (amy) and of two hybrid enzymes h(a12-m) delta f14 and h(a12-m) delta y13f14a were studied by spectroscopic and microcalorimetric measurements. h(a12-m) delta f14 is constructed by the fusion of 12 n-terminal amino acids of amy with amino acids 13-214 of mac, and by deletion of f14. in h(a12-m) delta y13f14a, the n-terminal region of mac is exchanged again ... | 1996 | 8931144 |
| production of free amino acids by some earthworm-borne microorganisms. | production of free amino acids by some earthworm-borne microorganisms was investigated in three different synthetic media. among the fungi tried gliocladium roseum and heterocephallum aurantiacum; among bacteria screened bacillus macerans and b. mycoides; and among actinomycetes tested streptomyces rimosus, s. violans, s. antibiticus, s. corchorusii and s. atroolivaceus produced significant amount of free amino acids. no correlations could be observed between vegetative growth and free amino aci ... | 1995 | 8972137 |
| a novel synthesis method for cyclodextrins from maltose in water-organic solvent systems. | a novel enzymatic synthesis method of cyclodextrin (cd) from low-mol-wt maltose using cyclomaltodextrin glucanotransferase (cgtase) from bacillus macerans has been developed in various water-organic solvent systems. a beta-cd was synthesized in a two-phase system consisting of water and cyclohexane. however, no cds could be synthesized in an aqueous buffer solution. a maximal yield of beta-cd has been obtained at a cyclohexane content volume of 44%. this synthesis has been obtained only at low t ... | 1996 | 8984903 |
| [catheter associated infection by bacillus macerans in a patient with acute leukemia]. | | 1996 | 9053012 |
| cyclodextrins are not the major cyclic alpha-1,4-glucans produced by the initial action of cyclodextrin glucanotransferase on amylose. | the initial action of cyclodextrin glucanotransferase (cgtase, ec 2.4.1.19) from an alkalophilic bacillus sp. a2-5a on amylose was investigated. synthetic amylose was incubated with purified cgtase then terminated in the very early stage of the enzyme reaction. when the reaction mixture was treated with glucoamylase and the resulting glucoamylase-resistant glucans were analyzed with high performance anion exchange chromatography, cyclic alpha-1,4-glucans, with degree of polymerization ranging fr ... | 1997 | 9188466 |
| cloning and expression of an endo-1,3-1,4-beta-glucanase gene from bacillus macerans in lactobacillus reuteri. | strains of the gastrointestinal species lactobacillus reuteri were electrotransformed with plasmid constructs containing the endo-1,3-1,4-beta-glucanase gene (bglm) of bacillus macerans. the enzyme was expressed and secreted by the lactobacilli. a plasmid construct containing the bglm gene lacking its promoter was derived and was demonstrated to be useful as a promoter probe vector. | 1997 | 9251227 |
| crystal structures and properties of de novo circularly permuted 1,3-1,4-beta-glucanases. | the 1,3-1,4-beta-glucanases from bacillus macerans and bacillus licheniformis, as well as related hybrid enzymes, are stable proteins comprised of one compact jellyroll domain. their structures are studied in an effort to reveal the degree of redundancy to which the three-dimensional structure of protein domains is encoded by the amino acid sequence. for the hybrid 1,3-1,4-beta-glucanase h(a16-m), it could be shown recently that a circular permutation of the sequence giving rise to the variant c ... | 1998 | 9489923 |
| an assay method for glycogen debranching enzyme using new fluorogenic substrates and its application to detection of the enzyme in mouse brain. | an assay method for glycogen debranching enzyme involving fluorogenic dextrins as substrates was developed. two dextrins were prepared from 6-o-alpha-d-glucosyl-alpha-cyclodextrin and glucose by taking advantage of the action of bacillus macerans cyclodextrin glucanotransferase, and converted by pyridylamination to fluorogenic derivatives. structural analysis of the fluorogenic dextrins by fab-ms, partial acid hydrolysis, and glucoamylase digestion revealed that they were glcalpha1-4(glcalpha1-6 ... | 1998 | 9562628 |
| development of elisa and enzyme-linked immunofiltration assay (elifa) methods for monitoring cyclodextrin glycosyltransferase (cgtase) production and bacterial growth in bacillus macerans batch cultures. | immunochemical methods were developed for monitoring cyclodextrin (cd) glycosyltransferase (cgtase) production and growth of an industrial cd-producing bacillus macerans strain. extracellular concentrations of cgtase released into a non-transparent culture medium during a 44 h long fermentation were detected by an indirect antigen inhibition enzyme-linked immunosorbent assay (elisa). the elisa was sensitive (minimal detection level 6 ng ml-1) and highly reproducible (coefficients of variation < ... | 1998 | 9571798 |
| structure and function of the bacillus hybrid enzyme gluxyn-1: native-like jellyroll fold preserved after insertion of autonomous globular domain. | the 1,3-1,4-beta-glucanase from bacillus macerans (wtglu) and the 1, 4-beta-xylanase from bacillus subtilis (wtxyn) are both single-domain jellyroll proteins catalyzing similar enzymatic reactions. in the fusion protein gluxyn-1, the two proteins are joined by insertion of the entire xyn domain into a surface loop of cpmac-57, a circularly permuted variant of wtglu. gluxyn-1 was generated by protein engineering methods, produced in escherichia coli and shown to fold spontaneously and have both e ... | 1998 | 9618460 |
| genetic diversity of nifh gene sequences in paenibacillus azotofixans strains and soil samples analyzed by denaturing gradient gel electrophoresis of pcr-amplified gene fragments. | the diversity of dinitrogenase reductase gene (nifh) fragments in paenibacillus azotofixans strains was investigated by using molecular methods. the partial nifh gene sequences of eight p. azotofixans strains, as well as one strain each of the close relatives paenibacillus durum, paenibacillus polymyxa, and paenibacillus macerans, were amplified by pcr by using degenerate primers and were characterized by dna sequencing. we found that there are two nifh sequence clusters, designated clusters i a ... | 1998 | 9687429 |
| expression of the succinate dehydrogenase genes (sdhcab) from the facultatively anaerobic paenibacillus macerans during aerobic growth | paenibacillus (formerly bacillus) macerans is capable of succinate oxidation under oxic conditions and fumarate reduction under anoxic conditions. the reactions are catalyzed by different enzymes, succinate dehydrogenase (sdh) and fumarate reductase (frd). the genes encoding sdh (sdhcab) were analyzed. the gene products of sdha and sdhb were similar to the subunits of known sdh and frd enzymes. the hydrophobic subunit sdhc showed close sequence similarity to the class of sdh/frd enzymes containi ... | 1998 | 9732445 |
| conversion of cyclodextrin into high-amylose starch of low molecular mass by means of cyclodextrin glucanotransferase. | alpha-cyclodextrin (cd) was converted by bacillus macerans cyclodextrin glucanotransferase into highly insoluble high-amylose starch with a low average degree of polymerization (56-73), in yields as high as 78% over a wide range of temperatures (25-70 degreesc). ability to undergo this conversion was highly concentration-dependent. gamma-cd was also convertible in good yield; however, beta-cd was relatively resistant to conversion. degrees of polymerization and the percentages of amylose in the ... | 1998 | 9799720 |
| effects of mutations in the starch-binding domain of bacillus macerans cyclodextrin glycosyltransferase. | cyclodextrin glycosyltransferase (cgtase) is an industrially important enzyme that produces cyclodextrins (cd) from starch by intramolecular transglycosylation. cgtase consists of five globular domains labeled a through e. to better understand the role of domain e in cgtase catalysis, we have constructed several mutants of bacillus macerans cgtase. removing the entire e domain resulted in an inactive enzyme. adding six amino acids between domains d and e caused a decrease in activity and thermos ... | 1998 | 9828462 |
| organization of genes for tetrapyrrole biosynthesis in gram--positive bacteria. | clusters of genes encoding enzymes for tetrapyrrole biosynthesis were cloned from bacillus sphaericus, bacillus stearothermophilus, brevibacillus brevis and paenibacillus macerans. the sequences of all hemx genes found, and of a 6.3 kbp hem gene cluster from p. macerans, were determined. the structure of the hem gene clusters was compared to that of other gram-positive bacteria. the bacillus and brevibacillus species have a conserved organization of the genes hemaxcdbl, required for biosynthesis ... | 1999 | 10217486 |
| characterization of the bacillus macerans cyclodextrin glucanotransferase overexpressed in escherichia coli. | cyclodextrin glucanotransferase (cgtase, ec 2. 4. 1. 19) converts starch and related alpha-1,4-glucans to cyclodextrin (cd). our previous studies of the enzyme have suggested that e344 on the polypeptide is crucial to the enzyme activity. mutational analysis of cgtase was performed to confirm this idea. three mutant cgtases containing either e344d, e344k or e344l substitution were overexpressed in escherichia coli. however, only the wild-type and e344d cgtases became soluble when expressed at 20 ... | 1999 | 10420654 |
| comparative phylogeny of rrs and nifh genes in the bacillaceae. | the rrs (16s rdna) gene sequences of nitrogen-fixing endospore-forming bacilli isolated from the rhizosphere of wheat and maize were determined in order to infer their phylogenetic position in the bacillaceae. these rhizosphere strains form a monophyletic cluster with paenibacillus azotofixans, paenibacillus polymyxa and paenibacillus macerans. two of them (rsa19 and tod45) had previously been identified as bacillus circulans (group 2) by phenotypic characterization (api 50ch). evidence for nitr ... | 1999 | 10425751 |
| protein-carbohydrate interactions defining substrate specificity in bacillus 1,3-1,4-beta-d-glucan 4-glucanohydrolases as dissected by mutational analysis. | the carbohydrate-binding site of bacillus macerans 1,3-1, 4-beta-d-glucan 4-glucanohydrolase has been analyzed through a mutational analysis to probe the role of protein-carbohydrate interactions defining substrate specificity. amino acid residues involved in substrate binding were proposed on the basis of a modeled enzyme-substrate complex [hahn, m., keitel, t., and heinemann, u. (1995) eur. j. biochem. 232, 849-859]. the effects of the mutations at 15 selected residues on catalysis and binding ... | 1999 | 10587432 |
| genetic and biochemical diversity among isolates of paenibacillus alvei cultured from australian honeybee (apis mellifera) colonies. | twenty-five unique cfoi-generated whole-cell dna profiles were identified in a study of 30 paenibacillus alvei isolates cultured from honey and diseased larvae collected from honeybee (apis mellifera) colonies in geographically diverse areas in australia. the fingerprint patterns were highly variable and readily discernible from one another, which highlighted the potential of this method for tracing the movement of isolates in epidemiological studies. 16s rrna gene fragments (length, 1,416 bp) f ... | 2000 | 10698777 |
| cycloamylose (cyclodextrin) glucanotransferase degrades intact granules of potato raw starch. | cycloamylose (cyclodextrin) glucanotransferase (ec 2.4.1.19, cgtase) originated from bacillus macerans degraded intact granules of potato raw starch and converted them into cyclodextrins (cds). the degradation required sufficient stirring of starch-cgtase suspension. the morphology of the degraded starch granules was unique; that is, the inner part of the granules was observed by scanning electron microscope to be more susceptible to cgtase than the outer part. effects of ph, temperature, starch ... | 2000 | 10725182 |
| hydrolytic action of alpha-amylase on high-amylose starch of low molecular mass. | high-amylose starches of low average degree of polymerization (d-p 61-71), formed as fine granules by interaction of bacillus macerans cyclodextrin glucanotransferase with alpha-cyclodextrin (cd) at 2-70 degrees c, are highly insoluble in water and not gelatinizable under normal cooking conditions (100 degrees c). samples of cd-derived starches, both cooked and uncooked, were subjected to hydrolysis in vitro by human salivary alpha-amylase at 37 degrees c under conditions chosen to resemble thos ... | 2000 | 10814586 |
| [new cyclomaltodextrin-glucanotransferases, produced by bacillus macerans]. | for the first time, microorganisms producing cyclomaltodextrin glucan transferases (cgt, ec 2.4.1.19) were isolated from soil samples of various ecogeographical regions. these microorganisms were identified as bacillus macerans. the enzymes were purified by affinity chromatography on a alpha-cyclodextrin polymer and gel filtration on biogel p-450 and proved to be electrophoretically homogeneous. some of their physicochemical and biochemical properties are reported. | 2000 | 10994187 |
| molecular characterization of cycloinulooligosaccharide fructanotransferase from bacillus macerans. | cycloinulooligosaccharide fructanotransferase (cftase) converts inulin into cyclooligosaccharides of beta-(2-->1)-linked d-fructofuranose by catalyzing an intramolecular transfructosylation reaction. the cftase gene was cloned and characterized from bacillus macerans cfc1. the cftase gene encoded a polypeptide of 1,333 amino acids with a calculated mr of 149,563. western blot and zymography analyses revealed that the cftase with a molecular mass of 150 kda (cft150) was processed (between ser389 ... | 2001 | 11157277 |
| comparative study of the cyclization reactions of three bacterial cyclomaltodextrin glucanotransferases. | the actions of cyclomaltodextrin glucanotransferases (cgtase; ec 2.4.1.19) from alkalophilic bacillus sp. strain a2-5a (a2-5a cgtase), bacillus macerans (bmac cgtase), and bacillus stearothermophilus (bste cgtase) on amylose were investigated. all three enzymes produced large cyclic alpha-1,4-glucans (cycloamyloses) at the early stage of the reaction, but these were subsequently converted into smaller cycloamyloses. however, the rates of this conversion differed among the three enzymes. the prod ... | 2001 | 11282590 |
| paenibacillus macerans pseudobacteremia resulting from contaminated blood culture bottles in a neonatal intensive care unit. | paenibacillus species are gram-positive, rod-shaped, spore-forming aerobes that are abundant in nature and closely related to bacillus. between june 24 and june 30, 1999, 8 neonates in our neonatal intensive care unit had positive blood cultures for paenibacillus macerans. this cluster of positive blood cultures with an unusual pathogen suggested a pseudoepidemic. investigation revealed that the most likely etiology of the pseudobacteremia was environmental contamination of the rubber stoppers i ... | 2001 | 11287883 |
| [establishment of the phylogenetic relationship between the microbial producers of cyclodextrin glucanotransferases using their complete amino acid sequences]. | a phylogenetic tree was constructed on the basis of the amino acid sequences of the known cyclodextrin glucanotransferases (cdgts), including those deduced from the nucleotide sequences of bacillus sp. strain 6.6.3 and paenibacillus macerans ib-7 genes encoding alpha- and beta-cdgts. the tree clearly demonstrates the existence of distinct phylogenetic groups of cdgt-producing microorganisms and the divergence of the alpha-, beta-, and gamma-cdgt produced by microorganisms from the genera bacillu ... | 2000 | 11315672 |
| trapping of a covalent enzyme intermediate in the reaction of bacillus macerans cyclomaltodextrin glucanyltransferase with cyclomaltohexaose. | the mechanism of catalysis of bacillus macerans cyclomaltodextrin glucanyltransferase (cgtase, ec 2.4.1.19) was studied by trapping and isolating a covalent-enzyme intermediate. cgtase catalyzes an acceptor or coupling reaction between cyclomaltohexaose and a carbohydrate acceptor such as d-glucose. cgtase was incubated with 3h-labeled cyclomaltohexaose in the absence of any added acceptor. after 30 s of reaction, the enzyme was rapidly denatured and precipitated by the addition of 10% trifluoro ... | 2001 | 11675025 |
| characterization of polycationic amino acids fusion systems for ion-exchange purification of cyclodextrin glycosyltransferase from recombinant escherichia coli. | fusion proteins with charged polycationic amino acid tails were constructed for the purpose of simple ion-exchange purification with high purity. a number of positively charged lysine and arginine tails were fused to the c-terminus of cyclodextrin glycosyltransferase (cgtase) derived from bacillus macerans and expressed in escherichia coli. the ionic binding forces provided by the tails allowed the selective recovery of cgtase from recombinant e. coli cell extracts, while cgtase by itself could ... | 2002 | 11934300 |
| bacillus macerans cyclomaltodextrin glucanotransferase transglycosylation reactions with different molar ratios of d-glucose and cyclomaltohexaose. | it was found that bacillus macerans cyclomaltodextrin glucanotransferase (cgtase) reacts with cyclomaltohexaose (alpha-cyclodextrin, alpha-cd) to give a series of cyclomaltooligosaccharides (cyclomaltodextrins, cds), having seven to more than 20 d-glucose residues and maltooligosaccharides (maltodextrins, mds) from g5 to g12+. when d-glucose (glc) was added to the alpha-cd at very low molar ratios (1:100) of glc to alpha-cd, the predominant products (95%) were cds, some of which were macrocyclic ... | 2002 | 12433489 |
| an investigation into the microflora of heroin. | in 2000, an unusual increase of morbidity and mortality among illegal injecting drug users in the uk and ireland was reported and clostridium novyi was identified as the likely source of the serious infection, although infections due to c. botulinum and bacillus cereus were also reported. because heroin was a possibile source of infection, this study investigated the microflora of heroin samples seized in england during 2000 and 2002. two methods were developed for the examination of the microfl ... | 2002 | 12448685 |
| molecular characterization of the operon comprising the spoiv gene of bacillus megaterium dsm319 and generation of a deletion mutant. | according to sequence analysis, the spoiv-locus of bacillus megaterium dsm 319 is 1,185 bp long; it is the second gene of a sporulation operon, which altogether contains three open reading frames. the orf preceding spoiv encodes a putative polypeptide with 94 amino acids; the 3rd orf of the operon has 972 bp corresponding to 324 amino acids. the operon is flanked on both sides by palindromic sequences, probably representing rho-independent terminators. a primer extension analysis revealed that m ... | 1998 | 12501411 |
| crystallization and preliminary x-ray diffraction studies of alpha-cyclodextrin glucanotransferase isolated from bacillus macerans. | single crystals of alpha-cyclodextrin glucanotransferase isolated from bacillus macerans have been grown with polyethylene glycol 6000 as a precipitating agent by sitting-drop vapour diffusion at room temperature. the crystals were suitable for x-ray analysis and diffracted to at least 2.0 a (space group p2(1)2(1)2(1)), with unit-cell parameters a = 66.79 (2), b = 79.66 (1), c = 141.16 (1) a. assuming the asymmetric cell to be occupied by a monomer of 74 kda, the unit cell contains 42.6% solvent ... | 2003 | 12554949 |
| effect of fusaric acid and phytoanticipins on growth of rhizobacteria and fusarium oxysporum. | suppression of soilborne diseases by biocontrol agents involves complex interactions among biocontrol agents and the pathogen and between these microorganisms and the plant. in general, these interactions are not well characterized. in this work, we studied (i) the diversity among strains of fluorescent pseudomonas spp., bacillus spp., and paenibacillus sp. for their sensitivity to fusaric acid (fac) and phytoanticipins from different host plants, (ii) the diversity of pathogenic and nonpathogen ... | 2002 | 12556125 |
| pseudobacteraemia in a patient with neutropenic fever caused by a novel paenibacillus species: paenibacillus hongkongensis sp. nov. | to characterise a strain of gram negative aerobic straight or slightly curved rods (hku3) isolated from the blood culture of a 9 year old chinese boy with neutropenic fever and pseudobacteraemia. | 2003 | 12560460 |
| starch-binding domain shuffling in aspergillus niger glucoamylase. | aspergillus niger glucoamylase (ga) consists mainly of two forms, gai [from the n-terminus, catalytic domain + linker + starch-binding domain (sbd)] and gaii (catalytic domain + linker). these domains were shuffled to make rgai (sbd + linker + catalytic domain), rgaideltal (sbd + catalytic domain) and rgaii (linker + catalytic domain), with domains defined by function rather than by tertiary structure. in addition, paenibacillus macerans cyclomaltodextrin glucanotransferase sbd replaced the clos ... | 2003 | 12915730 |
| studies on the metabolic function of biotin. v. depression of beta-hydroxybutyrate dehydrogenating activity in biotin-deficient bacillus macerans. | | 1961 | 13727394 |
| kinetics of dry rupture of bacterial spores in the presence of salt. | sacks, l. e. (u.s. department of agriculture, albany, calif.), peter b. percell, richard s. thomas, and glen f. bailey. kinetics of dry rupture of bacterial spores in the presence of salt. j. bacteriol. 87:952-960. 1964.-the kinetics of breaking spores in the dry state by use of an excess of sodium chloride and a steel ball in a shaking device were investigated. under most conditions, disruption is a first-order process. the disruption-rate constant varies directly with the weight of the ball an ... | 1964 | 14137636 |
| formation and degradation of cyclic dextrins by intracellular enzymes of bacillus macerans. | the enzymes of bacillus macerans that participate in the formation and degradation of schardinger dextrins were shown to be intracellular. bacillus macerans amylase converts starch into cyclic dextrins. a newly discovered enzyme, cyclodextrinase, catalyzes the degradation of cyclic dextrins. | 1964 | 14202462 |
| internal membranous structure in bacillus macerans spores. | | 1965 | 14291602 |
| pyruvate exchange reactions in bacillus macerans. | | 1959 | 14399275 |
| 16s rdna targeted pcr for the detection of paenibacillus macerans. | to develop a pcr detection method, which could be used for the detection of paenibacillus macerans in environmental samples or to help the identification of strains suspected to belong to this species. | 2003 | 14633114 |
| identification and characterization of a novel inulin binding module (ibm) from the cftase of bacillus macerans cfc1. | a novel inulin-binding module (ibm), which was identified from the n-terminal region of the cycloinulinooligosaccharide fructanotransferase (cftase) in bacillus macerans cfc1, was characterized using the discrete entity of ibm produced by the recombinant escherichia coli strains. deletion analyses located the inulin binding activity in the n-terminal region between 241 and 389 amino acid residues, which was removed from the mature enzyme by processing when secreted from the b. macerans cfc1 cell ... | 2004 | 15109727 |
| enzymatic synthesis of two salicin analogues by reaction of salicyl alcohol with bacillus macerans cyclomaltodextrin glucanyltransferase and leuconostoc mesenteroides b-742cb dextransucrase. | beta-salicin is a naturally occurring glycoside found in the bark of poplar and willow trees. ancient man used it as an analgesic and antipyretic. it has a d-glucopyranose unit attached by a beta-linkage to the phenolic hydroxyl of salicyl alcohol. two new salicin analogues have been enzymatically synthesized by transglycosylation reactions: (a) by the reaction of bacillus macerans cyclomaltodextrin glucanyltransferase with cyclomaltohexaose and salicyl alcohol, followed by reactions with alpha ... | 2004 | 15178396 |
| rhizosheath of sinai desert plants is a potential repository for associative diazotrophs. | among 42 plant species representing the flora of north sinai, two possessed sand grain sheath encasing the roots. they are panicum turgidum forssk. and stipagrostis scoparia (trin.and rupr.) dewinter. rhizosheaths, compared to surrounding free sand, accommodated higher population density of microorganisms including associative diazotrophs. isolates secured belonged to the species of bacillus circulans, paenib. macerans (bacillus macerans), enterobacter agglomerans, agrobacterium radiobacter and ... | 2004 | 15462528 |
| coexpression of folding accessory proteins for production of active cyclodextrin glycosyltransferase of bacillus macerans in recombinant escherichia coli. | coexpression of folding accessory proteins, molecular chaperones, and human peptidyl-prolyl cis-trans isomerase (ppiase) increased production of active cyclodextrin glycosyltransferase (cgtase) of bacillus macerans, which is otherwise mainly expressed as inclusion body in recombinant escherichia coli. the best partner for soluble expression of cgtase was found to be human ppiase followed by coexpression of dnak-dnaj-grpe together with groel-groes. such a significant enhancement by human ppiase c ... | 2005 | 15866731 |
| characterization of cyclodextrin glycosyltransferase of the same gene expressed from bacillus macerans, bacillus subtilis, and escherichia coli. | the plasmid phg contains a cyclodextrin glycosyltransferase (cgtase) gene (cgt) derived from bacillus macerans. two transformants, bacillus subtilis (phg) and escherichia coli (phg), were found to produce cgtases with the same primary structure as the enzyme from b. macerans. however, the beta-cyclodextrin coupling activity of the cgtase from e. coli (phg) was 14-fold higher than that of the enzymes from the other strains. by contrast, no differences in alpha-cyclodextrin coupling activities wer ... | 2005 | 16076110 |
| construction of chimeric cyclodextrin glucanotransferases from bacillus circulans a11 and paenibacillus macerans iam1243 and analysis of their product specificity. | three dna fragments of 7919 base pairs containing genes for beta-cyclodextrin glucanotransferase (cgtase, ec 2.4.1.19), an iron iii dicitrate transport protein-like protein and a partial coding sequence for putative ferrichrome abc transporter from bacillus circulans a11 were cloned and sequenced (genbank accession af302787). the dna sequence contained a cgtase open reading frame of 2139 base pairs, which encoded a polypeptide of 713 amino acid residues. the signal peptide constituted the n-term ... | 2005 | 16084934 |